Research Topics
| M L SoginSummaryAffiliation: Marine Biological Laboratory Country: USA Publications
Research Grants
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Detail Information
Publications
Effect of PCR amplicon size on assessments of clone library microbial diversity and community structureJulie A Huber
Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA 02543, USA
Environ Microbiol 11:1292-302. 2009....
Accuracy and quality of massively parallel DNA pyrosequencingSusan M Huse
Josephine Bay Paul Center, Marine Biological Laboratory at Woods Hole, MBL Street, Woods Hole, MA 02543, USA
Genome Biol 8:R143. 2007....
Microbial diversity in the deep sea and the underexplored "rare biosphere"Mitchell L Sogin
Josephine Bay Paul Center, Marine Biological Laboratory at Woods Hole, 7 MBL Street, Woods Hole, MA 02543, USA
Proc Natl Acad Sci U S A 103:12115-20. 2006..Members of the rare biosphere are highly divergent from each other and, at different times in earth's history, may have had a profound impact on shaping planetary processes...
History assignment: when was the mitochondrion founded?M L Sogin
Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
Curr Opin Genet Dev 7:792-9. 1997....
Evolution of the protists and protistan parasites from the perspective of molecular systematicsM L Sogin
Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA
Int J Parasitol 28:11-20. 1998..We must place greater emphasis upon improved phylogenetic inference techniques and investigations of genomic diversity in protists...
Ancestral relationships of the major eukaryotic lineagesM L Sogin
Center for Molecular Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
Microbiologia 12:17-28. 1996..The molecular phylogenies suggest that multiple endosymbiotic events introduced plastids into discrete eukaryotic lineages...
Evaluating hypotheses of basal animal phylogeny using complete sequences of large and small subunit rRNAM Medina
Marine Biological Laboratory, Woods Hole, MA 02453, USA
Proc Natl Acad Sci U S A 98:9707-12. 2001..LSU sequence data reveal phylogenetic structure in a data set with limited taxon sampling. Continued accumulation of LSU sequences should increase our understanding of animal phylogeny...
Microbiology: eukaryotic diversity in Spain's River of FireLinda A Amaral Zettler
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
Nature 417:137. 2002..The diversity of these eukaryotes is much greater than that of prokaryotes, whose metabolism is responsible for the extreme environment...
Microbial diversity of hydrothermal sediments in the Guaymas Basin: evidence for anaerobic methanotrophic communitiesAndreas Teske
Biology Department, Woods Hole Oceanographic Institution, Massachusetts 02543, USA
Appl Environ Microbiol 68:1994-2007. 2002..This study stresses a high diversity among communities capable of anaerobic oxidation of methane...
Abundance and diversity of microbial life in ocean crustCara M Santelli
MIT WHOI Joint Program in Oceanography and Ocean Engineering, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, USA
Nature 453:653-6. 2008....
Phylogenetic characterization of epibiotic bacteria in the accessory nidamental gland and egg capsules of the squid Loligo pealei (Cephalopoda:Loliginidae)E Barbieri
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA
Environ Microbiol 3:151-67. 2001..pealei. The molecular approach allowed the visualization of alpha-proteobacteria as major constituents of a bacterial symbiosis within the reproductive system of the Loliginidae...
Phylogeny of the genera Entamoeba and Endolimax as deduced from small-subunit ribosomal RNA sequencesJ D Silberman
Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
Mol Biol Evol 16:1740-51. 1999..Finally, we show that the positions of some (fast-evolving) eukaryotic lineages are uncertain in trees constructed with models that make corrections for among-site rate variation...
Giardia lamblia expresses a proteobacterial-like DnaK homologH G Morrison
The Josephine Bay Paul Center of Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543-1015, USA
Mol Biol Evol 18:530-41. 2001..These data neither confirm nor reject the possibility that this gene is a relic of secondary mitochondrial loss; they leave open the possibility that it was acquired in a separate endosymbiotic event...
Ironing out the wrinkles in the rare biosphere through improved OTU clusteringSusan M Huse
Josephine Bay Paul Center, Marine Biological Laboratory at Woods Hole, 7 MBL Street, Woods Hole, MA 02543, USA
Environ Microbiol 12:1889-98. 2010..This new clustering method can reduce the OTU richness in environmental samples by as much as 30-60% but does not reduce the fraction of OTUs in long-tailed rank abundance curves that defines the rare biosphere...
Methanogen diversity evidenced by molecular characterization of methyl coenzyme M reductase A (mcrA) genes in hydrothermal sediments of the Guaymas BasinAshita Dhillon
Marine Biological Laboratory, The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, Massachusetts, USA
Appl Environ Microbiol 71:4592-601. 2005..Phylogenetic affiliations of mcrA and 16S rRNA clones with thermophilic and nonthermophilic cultured isolates indicate a mixed mesophilic and thermophilic methanogen community in the surficial Guaymas sediments...
The evolution of the Vahlkampfiidae as deduced from 16S-like ribosomal RNA analysisG Hinkle
Center for Molecular Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543
J Eukaryot Microbiol 40:599-603. 1993..The Vahlkampfiidae lineage is monophyletic, branches early along the eukaryotic line of descent, and is not a close relative of the multicellular amoebae that also reversibly transform from amoebae to flagellates...
Evolutionary relationships among "jakobid" flagellates as indicated by alpha- and beta-tubulin phylogeniesV P Edgcomb
Josephine Bay-Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
Mol Biol Evol 18:514-22. 2001..Relationships with the putatively deep-branching amitochondriate diplomonads remain uncertain...
The sequence of the Hartmannella vermiformis small subunit rRNA coding regionJ H Gunderson
Center for Molecular Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543
J Eukaryot Microbiol 41:481-2. 1994..The coding region is 1,840 nucleotides long, and is typical of eukaryotic rRNA genes in both size and composition. Different clones contained different nucleotides at three positions...
The nucleariid amoebae: more protists at the animal-fungal boundaryT A Nerad
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
J Eukaryot Microbiol 48:293-7. 2001..These nucleariids are unrelated to the lineage containing the testate filose amoebae (Testaceafilosia). Our findings expand the morphological and phylogenetic diversity of protists at the animal-fungal divergence...
Inferring protein function from genomic sequence: Giardia lamblia expresses a phosphatidylinositol kinase-related kinase similar to yeast and mammalian TORHilary G Morrison
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 1015, USA
Comp Biochem Physiol B Biochem Mol Biol 133:477-91. 2002..Our investigation of the Giardia genome suggests that PIK-related kinases are of ancient origin and are highly conserved...
Microbial population structures in the deep marine biosphereJulie A Huber
Josephine Bay Paul Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA
Science 318:97-100. 2007....
Genomic minimalism in the early diverging intestinal parasite Giardia lambliaHilary G Morrison
Marine Biological Laboratory, Woods Hole, MA 02543 1015, USA
Science 317:1921-6. 2007..This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite...
Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophileMark A Messerli
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA
J Exp Biol 208:2569-79. 2005..Our results indicate that the small increase in the rate of ATP consumption can account for maintenance of the transmembrane H+ gradient without the imposition of cell surface H+ barriers...
Molecular characterization of sulfate-reducing bacteria in a New England salt marshMichele Bahr
Marine Biological Laboratory, Woods Hole, MA 02543, USA
Environ Microbiol 7:1175-85. 2005..The sequence data from this study will allow for the design of probes or primers that can quantitatively assess the diverse range of sulfate reducers present in the environment...
Benthic eukaryotic diversity in the Guaymas Basin hydrothermal vent environmentVirginia P Edgcomb
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA
Proc Natl Acad Sci U S A 99:7658-62. 2002..g., phototrophs) are simply deposited by sedimentary processes. In contrast, bacterial populations in these sediments are primarily characteristic of anoxic, reduced, hydrocarbon-rich sedimentary habitats...
Isolated communities of Epsilonproteobacteria in hydrothermal vent fluids of the Mariana Arc seamountsJulie A Huber
Marine Biological Laboratory, Josephine Bay Paul Center, Woods Hole, MA 02543, USA
FEMS Microbiol Ecol 73:538-49. 2010..This suggests that there may be barriers to exchange and dispersal for these vent endemic microorganisms at hydrothermal seamounts of the Mariana Arc...
Phylogenetic relationships between the Acantharea and the Polycystinea: a molecular perspective on Haeckel's RadiolariaL A Zettler
The Josephine Bay Paul Center in Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA
Proc Natl Acad Sci U S A 94:11411-6. 1997..We conclude that the Actinopoda does not represent a monophyletic evolutionary assemblage and recommend that this taxonomic designation be discarded...
The evolutionary origins of eukaryotic protein disulfide isomerase domains: new evidence from the Amitochondriate protist Giardia lambliaA G McArthur
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts 02543-1015, USA
Mol Biol Evol 18:1455-63. 2001..The PDI complement of G. lamblia and yeast suggests that a combination of PDIs may be used as a redox chain analogous to that known for bacterial Dsb proteins...
From genes to genomes: beyond biodiversity in Spain's Rio TintoLinda A Amaral Zettler
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts, USA
Biol Bull 204:205-9. 2003..We predict that special properties of these ion transporters allow protists to survive in the Rio Tinto...
The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systemsErica Lasek-Nesselquist
Josephine Bay Paul Center for Comparative Evolution and Molecular Biology, MBL, 7 MBL St, Woods Hole, MA 02543, USA
Int J Parasitol 40:1063-74. 2010..duodenalis as these assemblages are from each other. The discovery of a previously uncharacterised lineage of G. duodenalis suggests that this parasite has more genetic diversity and perhaps a larger host range than previously believed...
Molecular characterization of sulfate-reducing bacteria in the Guaymas BasinAshita Dhillon
Marine Biological Laboratory, The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, NASA Astrobiology Institute, 7 MBL Street, Woods Hole, MA 02543, USA
Appl Environ Microbiol 69:2765-72. 2003..In addition, the dsrAB sequencing approach revealed a novel group of sulfate-reducing prokaryotes that could not be identified by 16S rRNA sequencing...
Genomic markers of ancient anaerobic microbial pathways: sulfate reduction, methanogenesis, and methane oxidationAndreas Teske
Biology Department, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, USA
Biol Bull 204:186-91. 2003....
Exploring microbial diversity and taxonomy using SSU rRNA hypervariable tag sequencingSusan M Huse
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America
PLoS Genet 4:e1000255. 2008..This technique allows the cost-effective exploration of changes in microbial community structure, including the rare biosphere, over space and time and can be applied immediately to initiatives, such as the Human Microbiome Project...
Serial analysis of V6 ribosomal sequence tags (SARST-V6): a method for efficient, high-throughput analysis of microbial community compositionDavid T Kysela
The Josephine Bay Paul Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA
Environ Microbiol 7:356-64. 2005..These results demonstrate the utility of SARST-V6 in collecting taxonomically informative data for high-throughput analysis of microbial communities...
Bacterial group II introns in a deep-sea hydrothermal vent environmentMircea Podar
Biology Department, Woods Hole Oceanographic Institution, Massachusetts 02543, USA
Appl Environ Microbiol 68:6392-8. 2002..These types of mobile genetic elements may prove useful in improving our understanding of bacterial genome evolution and may serve as valuable markers in comparative studies of bacterial communities...
Acinetobacter, Aeromonas and Trichococcus populations dominate the microbial community within urban sewer infrastructureJ L Vandewalle
Great Lakes WATER Institute, University of Wisconsin Milwaukee, 600 E Greenfield Ave, Milwaukee, WI 53204, USA
Environ Microbiol 14:2538-52. 2012..This work provides insight into microbial community composition and dynamics within the defined environment of urban sewer infrastructure...
Cloning and analysis of the CYP1A promoter from the atlantic killifish (Fundulus heteroclitus)Wade H Powell
Marine Biological Laboratory, Woods Hole, MA, USA
Mar Environ Res 58:119-24. 2004..These studies may ultimately shed light on the importance of P4501A activity in xenobiotic toxicity...
Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequencesChristophe Noël
Institut Pasteur, Unité Mixte INSERM IPL U547, 1 Rue du Professeur Calmette BP 245, 59019 Lille Cedex, France
Mol Biochem Parasitol 126:119-23. 2003
Evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, NADH oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to Giardia lamblia and Entamoeba histolyticaJulie E J Nixon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
Eukaryot Cell 1:181-90. 2002..In further support of lateral transfer, the G. lamblia NADH oxidase and adh3 genes appeared to have an evolutionary history distinct from those of E. histolytica...
Molecular characterization of Giardia intestinalis haplotypes in marine animals: variation and zoonotic potentialErica Lasek-Nesselquist
Department of Ecology and Evolutionary Biology, Brown University, 80 Waterman Street, Providence, Rhode Island 02912, USA
Dis Aquat Organ 81:39-51. 2008..The presence of G. intestinalis in marine ecosystems raises concerns about how this disease might be transmitted among different host species...
Molecular phylogeny of parabasalids inferred from small subunit rRNA sequences, with emphasis on the Devescovinidae and Calonymphidae (Trichomonadea)Delphine Gerbod
Laboratory of Microbiology, RIKEN (The Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
Mol Phylogenet Evol 25:545-56. 2002....
Evolutionary history of "early-diverging" eukaryotes: the excavate taxon Carpediemonas is a close relative of GiardiaAlastair G B Simpson
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada
Mol Biol Evol 19:1782-91. 2002..The phylogenetic position of Carpediemonas suggests that it will be valuable in interpreting the evolutionary significance of many molecular and cellular peculiarities of diplomonads...
Giardia lamblia: identification and characterization of Rab and GDI proteins in a genome survey of the ER to Golgi endomembrane systemT Dianne Langford
Division of Infectious Diseases, Department of Pathology, University of California, San Diego, CA 92103-8416, USA
Exp Parasitol 101:13-24. 2002..GDI associated with these structures, and with small vesicles found throughout the cytoplasm, consistent with GDI's key role in Rab cycling between organelles within the cell...
A spliceosomal intron in Giardia lambliaJulie E J Nixon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115-6018, USA
Proc Natl Acad Sci U S A 99:3701-5. 2002..If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia...
Iron-dependent hydrogenases of Entamoeba histolytica and Giardia lamblia: activity of the recombinant entamoebic enzyme and evidence for lateral gene transferJulie E J Nixon
Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Ave, Boston, Massachusetts, USA
Biol Bull 204:1-9. 2003..These results are discussed in the context of competing ideas for the origins of genes encoding fermentation enzymes of these protists...
Evolution of eukaryotic transcription: insights from the genome of Giardia lambliaAaron A Best
Department of Microbiology, University of Illinois at Urbana-Champaign, B103 Chemical and Life Sciences Laboratory, Urbana, Illinois 61801, USA
Genome Res 14:1537-47. 2004..Consequently, Giardia is predicted to be unique in many aspects of transcription initiation with respect to paradigms derived from studies in crown eukaryotes...
Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosisChristophe Noël
Unité Inserm U547, Institut Pasteur, 1 rue du Professeur Calmette, BP 245, 59019 Lille Cedex, France
J Clin Microbiol 43:348-55. 2005..Our results also strongly suggest the existence of numerous zoonotic isolates with frequent animal-to-human and human-to-animal transmissions and of a large potential reservoir in animals for infections in humans...
Annexin-like alpha giardins: a new cytoskeletal gene family in Giardia lambliaMalin E-L Weiland
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
Int J Parasitol 35:617-26. 2005..These localisation experiments suggest alpha-giardins play a role in cell motility, attachment and membrane stability...
Microbial biogeography along an estuarine salinity gradient: combined influences of bacterial growth and residence timeByron C Crump
Horn Point Laboratory, University of Maryland Center for Environmental Science, Cambridge, Maryland 21613, USA
Appl Environ Microbiol 70:1494-505. 2004....
Monopylocystis visvesvarai n. gen., n. sp. and Sawyeria marylandensis n. gen., n. sp.: two new amitochondrial heterolobosean amoebae from anoxic environmentsCharles J O'Kelly
Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575, USA
Protist 154:281-90. 2003..In phylogenetic analyses of small-subunit rRNA gene sequences, Monopylocystis visvesvarai, Sawyeria marylandensis and Psalteriomonas lanterna converge to form a single clade of non-aerobic (anaerobic/microaerophilic) heteroloboseans...
Two new small-subunit ribosomal RNA gene lineages within the subclass gymnamoebiaMichael T Peglar
American Type Culture Collection, Manassas, Virginia 20110, USA
J Eukaryot Microbiol 50:224-32. 2003..Unique cell surface features validate these as genera distinct from Vannella and Platyamoeba. Genetic and ultrastructural data are used to discuss the phylogenetic interrelationships for the taxa studied...
Heterotrophic Archaea dominate sedimentary subsurface ecosystems off PeruJennifer F Biddle
Pennsylvania State Astrobiology Research Center, Pennsylvania State University, University Park, PA 16802, USA
Proc Natl Acad Sci U S A 103:3846-51. 2006..Our study provides clues about the metabolic functionality of two cosmopolitan groups of uncultured Archaea...
Giardia lamblia RNA polymerase II: amanitin-resistant transcriptionVishwas Seshadri
Department of Microbiology, University of Arizona College of Medicine, Tucson, Arizona 85724-5049, USA
J Biol Chem 278:27804-10. 2003..lamblia...
Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial matRuth E Ley
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Appl Environ Microbiol 72:3685-95. 2006..The sequences determined in this study have been submitted to the GenBank database and assigned accession numbers DQ 329539 to DQ 331020, and DQ 397339 to DQ 397511...
Pelobionts are degenerate protists: insights from molecules and morphologyVirginia P Edgcomb
Mol Biol Evol 19:978-82. 2002
Research Grants
- Molecular Evolution of Eukaryotes: a protistan emphasisMitchell Sogin; Fiscal Year: 2007....
