Peter J S Smith

Summary

Affiliation: Marine Biological Laboratory
Country: USA

Publications

  1. doi request reprint Windows to cell function and dysfunction: signatures written in the boundary layers
    Peter J S Smith
    BioCurrents Research Center, Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    Bioessays 32:514-23. 2010
  2. ncbi request reprint Long-term culture of fully differentiated adult insect neurons
    P J Smith
    National Vibrating Probe Facility, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    J Neurosci Methods 69:113-22. 1996
  3. ncbi request reprint Self-referencing, non-invasive, ion selective electrode for single cell detection of trans-plasma membrane calcium flux
    P J Smith
    BioCurrents Research Center and the Laboratory for Reproductive Medicine, Woods Hole, Massachusetts 02543, USA
    Microsc Res Tech 46:398-417. 1999
  4. ncbi request reprint Noninvasive measurement of hydrogen and potassium ion flux from single cells and epithelial structures
    P J Smith
    BioCurrents Research Center, Woods Hole, Massachusetts 02543, USA
    Am J Physiol Cell Physiol 280:C1-11. 2001
  5. pmc The level of menadione redox-cycling in pancreatic β-cells is proportional to the glucose concentration: role of NADH and consequences for insulin secretion
    Emma Heart
    Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, MA 02543, United States
    Toxicol Appl Pharmacol 258:216-25. 2012
  6. doi request reprint Construction, theory, and practical considerations for using self-referencing of Ca(2+)-selective microelectrodes for monitoring extracellular Ca(2+) gradients
    Mark A Messerli
    BioCurrents Research Center, Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA
    Methods Cell Biol 99:91-111. 2010
  7. ncbi request reprint Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophile
    Mark A Messerli
    The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    J Exp Biol 208:2569-79. 2005
  8. doi request reprint Characterization of optimized Na+ and Cl- liquid membranes for use with extracellular, self-referencing microelectrodes
    Mark A Messerli
    BioCurrents Research Center, Program in Molecular Physiology, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    Anal Bioanal Chem 390:1355-9. 2008
  9. ncbi request reprint Rhythm of the beta-cell oscillator is not governed by a single regulator: multiple systems contribute to oscillatory behavior
    Emma Heart
    BioCurrents Research Center, Molecular Physiology Program, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Am J Physiol Endocrinol Metab 292:E1295-300. 2007
  10. ncbi request reprint O2 availability modulates transmembrane Ca2+ flux via second-messenger pathways in anoxia-tolerant hepatocytes
    S C Land
    BioCurrents Research Center, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    J Appl Physiol 82:776-83. 1997

Research Grants

  1. Ultra-micro oxygen sensor development
    Peter Smith; Fiscal Year: 2003
  2. Role of islet structure and function
    Peter Smith; Fiscal Year: 2004

Collaborators

Detail Information

Publications17

  1. doi request reprint Windows to cell function and dysfunction: signatures written in the boundary layers
    Peter J S Smith
    BioCurrents Research Center, Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    Bioessays 32:514-23. 2010
    ..We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task in hand...
  2. ncbi request reprint Long-term culture of fully differentiated adult insect neurons
    P J Smith
    National Vibrating Probe Facility, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    J Neurosci Methods 69:113-22. 1996
    ....
  3. ncbi request reprint Self-referencing, non-invasive, ion selective electrode for single cell detection of trans-plasma membrane calcium flux
    P J Smith
    BioCurrents Research Center and the Laboratory for Reproductive Medicine, Woods Hole, Massachusetts 02543, USA
    Microsc Res Tech 46:398-417. 1999
    ..Applications of the calcium selective probe are given, drawing on examples from the plant sciences, developmental biology, muscle physiology, and the neurosciences...
  4. ncbi request reprint Noninvasive measurement of hydrogen and potassium ion flux from single cells and epithelial structures
    P J Smith
    BioCurrents Research Center, Woods Hole, Massachusetts 02543, USA
    Am J Physiol Cell Physiol 280:C1-11. 2001
    ..The strengths of the self-referencing technique are reviewed with regard to biological applications, and the expansion of self-referencing probes to include electrochemical and enzyme-based sensors is discussed...
  5. pmc The level of menadione redox-cycling in pancreatic β-cells is proportional to the glucose concentration: role of NADH and consequences for insulin secretion
    Emma Heart
    Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, MA 02543, United States
    Toxicol Appl Pharmacol 258:216-25. 2012
    ..These data may explain the apparent dichotomy between the stimulatory and inhibitory effects of H(2)O(2) and menadione on insulin secretion...
  6. doi request reprint Construction, theory, and practical considerations for using self-referencing of Ca(2+)-selective microelectrodes for monitoring extracellular Ca(2+) gradients
    Mark A Messerli
    BioCurrents Research Center, Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA
    Methods Cell Biol 99:91-111. 2010
    ..We also discuss potential complications when using them to measure Ca(2+) gradients near the boundary layers of single cells and tissues...
  7. ncbi request reprint Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophile
    Mark A Messerli
    The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    J Exp Biol 208:2569-79. 2005
    ..Our results indicate that the small increase in the rate of ATP consumption can account for maintenance of the transmembrane H+ gradient without the imposition of cell surface H+ barriers...
  8. doi request reprint Characterization of optimized Na+ and Cl- liquid membranes for use with extracellular, self-referencing microelectrodes
    Mark A Messerli
    BioCurrents Research Center, Program in Molecular Physiology, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    Anal Bioanal Chem 390:1355-9. 2008
    ..Self-referencing with these ISMs will enable near-real-time ion flux measurements for Na+ and Cl-...
  9. ncbi request reprint Rhythm of the beta-cell oscillator is not governed by a single regulator: multiple systems contribute to oscillatory behavior
    Emma Heart
    BioCurrents Research Center, Molecular Physiology Program, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Am J Physiol Endocrinol Metab 292:E1295-300. 2007
    ..This supports the hypothesis that a high-fat environment might contribute to loss of regular oscillatory pattern in diabetic subjects, acting, at least in part, at the level of the single beta-cell...
  10. ncbi request reprint O2 availability modulates transmembrane Ca2+ flux via second-messenger pathways in anoxia-tolerant hepatocytes
    S C Land
    BioCurrents Research Center, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    J Appl Physiol 82:776-83. 1997
    ..These observations suggest modulatory control by oxygen over transmembrane Ca2+ efflux involving second-messenger systems in the hypoxic transition...
  11. pmc Ion trapping with fast-response ion-selective microelectrodes enhances detection of extracellular ion channel gradients
    Mark A Messerli
    BioCurrents Research Center, Cell Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Biophys J 96:1597-605. 2009
    ..This method provides a noninvasive means for functional mapping of channel location and density as well as for characterizing the properties of ion channels in the plasma membrane...
  12. pmc Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion
    Emma Heart
    BioCurrents Research Center, Marine Biological Laboratory, 7 MBL St, Lillie 219, Woods Hole, MA 02543, USA
    Am J Physiol Endocrinol Metab 296:E1354-62. 2009
    ..These data suggest that although ME1 overexpression augments anaplerosis and GSIS in INS-1 832/13 cells, it is not likely involved in MSSIS and GSIS in pancreatic islets...
  13. ncbi request reprint Chloride fluxes in lily pollen tubes: a critical reevaluation
    Mark A Messerli
    BioCurrents Research Center, Program in Molecular Physiology, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    Plant J 40:799-812. 2004
    ..These results lead to the conclusion that Cl- fluxes are not a significant component of pollen tube growth and Cl- itself is not required for growth...
  14. ncbi request reprint From genes to genomes: beyond biodiversity in Spain's Rio Tinto
    Linda A Amaral Zettler
    The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts, USA
    Biol Bull 204:205-9. 2003
    ..We predict that special properties of these ion transporters allow protists to survive in the Rio Tinto...
  15. ncbi request reprint Determination of single-cell oxygen consumption with impedance feedback for control of sample-probe separation
    Damon M Osbourn
    Program in Molecular Physiology, Marine Biological Laboratory, BioCurrents Research Center, Woods Hole, Massachusetts 02543, USA
    Anal Chem 77:6999-7004. 2005
    ..Furthermore, it is shown that the sample-probe distance can be measured for approach to single cells on the order of 10-15-microm diameter and 5-microm height, with 100-nm resolution...
  16. ncbi request reprint Apoptosis recruits two-pore domain potassium channels used for homeostatic volume regulation
    James R Trimarchi
    Laboratory for Reproductive Medicine, Lillie Bldg, Marine Biological Laboratory, 7 MBL St, Woods Hole, MA 02543, USA
    Am J Physiol Cell Physiol 282:C588-94. 2002
    ..We propose that K(2P) channels are the pathway by which K+ effluxes during AVD and RVD and that apoptosis co-opts mechanisms more routinely employed for homeostatic cell volume regulation...
  17. ncbi request reprint Development and application of a self-referencing glucose microsensor for the measurement of glucose consumption by pancreatic beta-cells
    S K Jung
    Marine Biological Laboratory, BioCurrents Research Center, Woods Hole, Massachusetts 02543, USA
    Anal Chem 73:3759-67. 2001
    ..Further, in obtaining glucose flux measurements from small clusters of cells, these are the first recordings of the real-time dynamic of glucose movements in a biological microenvironment...

Research Grants4

  1. Ultra-micro oxygen sensor development
    Peter Smith; Fiscal Year: 2003
    ..abstract_text> ..
  2. Role of islet structure and function
    Peter Smith; Fiscal Year: 2004
    ....