Robert H Kutner

Summary

Affiliation: Louisiana State University Health Sciences Center
Country: USA

Publications

  1. pmc A doxycycline-inducible urokinase receptor (uPAR) upregulates uPAR activities including resistance to anoikis in human prostate cancer cell lines
    Mohammad Hasanuzzaman
    LSU Department of Comparative Biomedical Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Mol Cancer 6:34. 2007
  2. pmc Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study
    Krzysztof Pluta
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    BMC Biotechnol 7:41. 2007
  3. pmc Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography
    Robert H Kutner
    Gene Therapy Program, Vector Core, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    BMC Biotechnol 9:10. 2009
  4. doi request reprint Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 10:469-83. 2008
  5. doi request reprint A comparative analysis of constitutive and cell-specific promoters in the adult mouse hippocampus using lentivirus vector-mediated gene transfer
    Hitoshi Kuroda
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    J Gene Med 10:1163-75. 2008
  6. doi request reprint Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors
    Robert H Kutner
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, Louisiana 70112, USA
    Nat Protoc 4:495-505. 2009
  7. pmc Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus
    Xian Yang Zhang
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Retrovirology 7:3. 2010
  8. doi request reprint Production of lentiviral vectors in protein-free media
    Hitoshi Kuroda
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
    Curr Protoc Cell Biol . 2011
  9. doi request reprint Generation of domestic transgenic cloned kittens using lentivirus vectors
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 11:167-76. 2009
  10. doi request reprint Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection
    Hitoshi Kuroda
    Louisiana State University Health Sciences Center, New Orleans, 70112, USA
    J Virol Methods 157:113-21. 2009

Collaborators

  • Jakob Reiser
  • William B Klimstra
  • Agnieszka Bialkowska
  • Hitoshi Kuroda
  • Martha C Gómez
  • Xian Yang Zhang
  • Michael P Marino
  • David M Ricks
  • Justine Lyons
  • Leslie A Lyons
  • Nicolas G Bazan
  • Betsy L Dresser
  • Cherie Dumas
  • Krzysztof Pluta
  • Mohammad Hasanuzzaman
  • Lili Bao
  • Mark T Ruhe
  • Charles Earle Pope
  • Monica Lopez
  • Mark Ruhe
  • C Earle Pope
  • Siamak Agha-Mohammadi
  • Inder Sehgal
  • William Diehl
  • Vijayendra Jaligam
  • Stephen P Kantrow

Detail Information

Publications11

  1. pmc A doxycycline-inducible urokinase receptor (uPAR) upregulates uPAR activities including resistance to anoikis in human prostate cancer cell lines
    Mohammad Hasanuzzaman
    LSU Department of Comparative Biomedical Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Mol Cancer 6:34. 2007
    ..Our objective in this study was to generate and test an inducible lentiviral system capable of expressing uPAR and DsRed fluorescent protein in human prostate cancer cell lines...
  2. pmc Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study
    Krzysztof Pluta
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    BMC Biotechnol 7:41. 2007
    ....
  3. pmc Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography
    Robert H Kutner
    Gene Therapy Program, Vector Core, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    BMC Biotechnol 9:10. 2009
    ..However, despite significant progress, the production and concentration of high-titer, high-quality LV vector stocks is still cumbersome and costly...
  4. doi request reprint Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 10:469-83. 2008
    ..Consequently, the number of embryos that cleaved, implanted, and developed to term-gestation and POU5F1 expression in derived blastocysts indirectly was affected...
  5. doi request reprint A comparative analysis of constitutive and cell-specific promoters in the adult mouse hippocampus using lentivirus vector-mediated gene transfer
    Hitoshi Kuroda
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    J Gene Med 10:1163-75. 2008
    ..A key advantage of such approaches is that specific brain regions implicated in a particular disease can be independently targeted...
  6. doi request reprint Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors
    Robert H Kutner
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, Louisiana 70112, USA
    Nat Protoc 4:495-505. 2009
    ..The vector production and titration protocol described here can be completed within 8 d...
  7. pmc Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus
    Xian Yang Zhang
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Retrovirology 7:3. 2010
    ....
  8. doi request reprint Production of lentiviral vectors in protein-free media
    Hitoshi Kuroda
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
    Curr Protoc Cell Biol . 2011
    ..The vector production and titration protocol described here can be completed within 8 days...
  9. doi request reprint Generation of domestic transgenic cloned kittens using lentivirus vectors
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 11:167-76. 2009
    ..In summary, we report the birth of transgenic cloned kittens produced by LV vector-mediated transduction of donor cells and confirm that cloned kittens express the EGFP reporter transgene in all body tissues...
  10. doi request reprint Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection
    Hitoshi Kuroda
    Louisiana State University Health Sciences Center, New Orleans, 70112, USA
    J Virol Methods 157:113-21. 2009
    ..As a consequence, large-scale lentiviral vector stocks can now be generated with fewer batch-to-batch variations and at reduced costs and with less labor compared to the standard protocols...
  11. ncbi request reprint Stable transgene expression in tumors and metastases after transduction with lentiviral vectors based on human immunodeficiency virus type 1
    Lili Bao
    Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    Hum Gene Ther 15:445-56. 2004
    ..These results demonstrate the usefulness of HIV-1-based lentiviral vectors for sustained and stable transgene expression in human lung and breast cancer cell lines in vitro and in vivo...