Andrew Rm Bradbury

Summary

Affiliation: Los Alamos National Laboratory
Country: USA

Publications

  1. ncbi request reprint Antibody Engineering--IBC's 17th annual international conference. Antibody engineering and immunotherapeutics for the 21st century
    Andrew Bradbury
    Los Alamos National Laboratory, Biosciences Division, MS M888, TA 43, HRL 1, Building 1, Los Alamos, NM 87545, USA
    IDrugs 10:169-72. 2007
  2. ncbi request reprint Predicting antigenic peptides suitable for the selection of phage antibodies
    Peter Pavlik
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Hum Antibodies 12:99-112. 2003
  3. pmc Eliminating helper phage from phage display
    L Chasteen
    B Division, Los Alamos National Laboratory MS M888, Los Alamos, NM 87545, USA
    Nucleic Acids Res 34:e145. 2006
  4. pmc Using phage and yeast display to select hundreds of monoclonal antibodies: application to antigen 85, a tuberculosis biomarker
    Fortunato Ferrara
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America
    PLoS ONE 7:e49535. 2012
  5. pmc Development of phage-based single chain Fv antibody reagents for detection of Yersinia pestis
    Antonietta M Lillo
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America
    PLoS ONE 6:e27756. 2011
  6. pmc Fluorescent labeling of antibody fragments using split GFP
    Fortunato Ferrara
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America
    PLoS ONE 6:e25727. 2011
  7. pmc A comprehensive analysis of filamentous phage display vectors for cytoplasmic proteins: an analysis with different fluorescent proteins
    Nileena Velappan
    B Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Nucleic Acids Res 38:e22. 2010
  8. pmc Antibody binding loop insertions as diversity elements
    Csaba Kiss
    HCDR3s as diversity elements, Los Alamos National Laboratory, Los Alamos, NM, USA
    Nucleic Acids Res 34:e132. 2006
  9. ncbi request reprint Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes
    Devin W Close
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
    BMC Microbiol 13:270. 2013
  10. pmc Filtering "genic" open reading frames from genomic DNA samples for advanced annotation
    Sara D'Angelo
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
    BMC Genomics 12:S5. 2011

Collaborators

  • Daniele Sblattero
  • Sandeep Kumar
  • Steven W Graves
  • John McCafferty
  • Pawel Listwan
  • Sara D'Angelo
  • James D Marks
  • Stefan Dubel
  • Norman A Doggett
  • Michael L Robinson
  • Peter Pavlik
  • Fortunato Ferrara
  • Cliff Han
  • Nileena Velappan
  • Csaba Kiss
  • Roberto Marzari
  • Leslie Chasteen
  • Devin W Close
  • Antonietta M Lillo
  • Robert W Siegel
  • Geoffrey S Waldo
  • Emanuele Pesavento
  • Joanne Ayriss
  • Roberto Di Niro
  • L Chasteen
  • Jorge Sepulveda
  • Hajnalka E Daligault
  • Armand E K Dichosa
  • Timothy C Sanchez
  • Cliff S Han
  • Ashlynn R Daughton
  • Srinivas Iyer
  • Krista G Reitenga
  • BASIL SWANSON
  • Leslie A Naranjo
  • Harshini Mukundan
  • Tiziano Gaiotto
  • Joanne E Ayriss
  • Yulin Shou
  • Hugh E Fisher
  • Michelle Longmire
  • Marco Stebel
  • Oscar Burrone
  • Paola Secco
  • Travis Woods
  • Fiorella Florian
  • Sergio Crovella
  • Marco Bestagno
  • Federica Ziller
  • MingHua Dai
  • Milan Ovecka
  • P Pavlik
  • Rhiannon Nolan
  • Rosa Valero
  • M Lisa Phipps
  • Hugh Fisher
  • J Ayriss
  • Jennifer S Martinez
  • Hulin Jin
  • Colin Collins
  • Oscar R Burrone
  • Vittorio Verzillo
  • Paolo Macor
  • Fabio Fischetti
  • Renato Gennaro
  • Francesco Tedesco

Detail Information

Publications24

  1. ncbi request reprint Antibody Engineering--IBC's 17th annual international conference. Antibody engineering and immunotherapeutics for the 21st century
    Andrew Bradbury
    Los Alamos National Laboratory, Biosciences Division, MS M888, TA 43, HRL 1, Building 1, Los Alamos, NM 87545, USA
    IDrugs 10:169-72. 2007
  2. ncbi request reprint Predicting antigenic peptides suitable for the selection of phage antibodies
    Peter Pavlik
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Hum Antibodies 12:99-112. 2003
    ..This is likely to have considerable utility in functional genomics and proteomics where it should be possible to select antibodies against gene products on the basis of deduced amino acid sequence in a high throughput fashion...
  3. pmc Eliminating helper phage from phage display
    L Chasteen
    B Division, Los Alamos National Laboratory MS M888, Los Alamos, NM 87545, USA
    Nucleic Acids Res 34:e145. 2006
    ....
  4. pmc Using phage and yeast display to select hundreds of monoclonal antibodies: application to antigen 85, a tuberculosis biomarker
    Fortunato Ferrara
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America
    PLoS ONE 7:e49535. 2012
    ..One of the bottlenecks in the direct detection of such bacterial targets is the availability of robust, sensitive, specific antibodies...
  5. pmc Development of phage-based single chain Fv antibody reagents for detection of Yersinia pestis
    Antonietta M Lillo
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America
    PLoS ONE 6:e27756. 2011
    ..Different forms of the selected antibodies were used to establish assays for recombinant F1 antigen and Y. pestis detection...
  6. pmc Fluorescent labeling of antibody fragments using split GFP
    Fortunato Ferrara
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America
    PLoS ONE 6:e25727. 2011
    ..We anticipate this system will be of widespread interest in antibody engineering and in vitro display systems...
  7. pmc A comprehensive analysis of filamentous phage display vectors for cytoplasmic proteins: an analysis with different fluorescent proteins
    Nileena Velappan
    B Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Nucleic Acids Res 38:e22. 2010
    ..This vector was able to display less robust forms of GFP, including ones with inserted epitopes, as well as fluorescent proteins of the Azami green series. It was also functional in mock selection experiments...
  8. pmc Antibody binding loop insertions as diversity elements
    Csaba Kiss
    HCDR3s as diversity elements, Los Alamos National Laboratory, Los Alamos, NM, USA
    Nucleic Acids Res 34:e132. 2006
    ....
  9. ncbi request reprint Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes
    Devin W Close
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
    BMC Microbiol 13:270. 2013
    ..Empirical methods that complement rapidly improving bioinformatic tools will improve characterization of microbiomes and facilitate better genome coverage for low abundance microbes...
  10. pmc Filtering "genic" open reading frames from genomic DNA samples for advanced annotation
    Sara D'Angelo
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
    BMC Genomics 12:S5. 2011
    ....
  11. pmc Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models
    Roberto Di Niro
    Department of Biology, University of Trieste, Trieste, Italy
    BMC Biotechnol 7:46. 2007
    ..In the case of Celiac Disease, the immune response is directed against the autoantigen tissue transglutaminase. However, despite numerous studies, the role of these antibodies in the pathogenesis of this disease has not been elucidated...
  12. ncbi request reprint scFvs and beyond
    Andrew Bradbury
    Los Alamos National Laboratory, Los Alamos, New Mexico, USA
    Drug Discov Today 8:737-9. 2003
  13. ncbi request reprint Antibodies in proteomics II: screening, high-throughput characterization and downstream applications
    Andrew Bradbury
    B Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Trends Biotechnol 21:312-7. 2003
    ..Such protocols need to be adapted for broader use, for example, with antibody fragments or other binders generated by display technologies, because it is unlikely that traditional approaches will provide the required throughput...
  14. ncbi request reprint Antibodies in proteomics I: generating antibodies
    Andrew Bradbury
    B Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Trends Biotechnol 21:275-81. 2003
    ..This review--the first of two--examines the strategies available for in vitro antibody selection. The second review discusses the adaptation of these methods to high throughput and the uses to which antibodies, once derived, can be put...
  15. ncbi request reprint Antibodies from phage antibody libraries
    Andrew R M Bradbury
    Bioscience Division, Los Alamos National Laboratory, TA 43, HRL 1, MS M888, NM 87545, USA
    J Immunol Methods 290:29-49. 2004
  16. pmc Beyond natural antibodies: the power of in vitro display technologies
    Andrew R M Bradbury
    Los Alamos National Laboratory, Los Alamos, New Mexico, USA
    Nat Biotechnol 29:245-54. 2011
    ..The amenability of in vitro display to high-throughput applications broadens the prospects for their wider use in basic and applied research...
  17. ncbi request reprint A 360-kb interchromosomal duplication of the human HYDIN locus
    Norman A Doggett
    DOE Joint Genome Institute and Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Genomics 88:762-71. 2006
    ..1. Both copies of the HYDIN gene are expressed in alternatively spliced transcripts. Elucidation of the role of HYDIN in human disease susceptibility will require careful discrimination among the paralogous copies...
  18. ncbi request reprint High-throughput screening of single-chain antibodies using multiplexed flow cytometry
    Joanne Ayriss
    Bioscience Division, Mail Stop M888, Los Alamos National Laboratory, P O Box 1663, Los Alamos, New Mexico 87545, USA
    J Proteome Res 6:1072-82. 2007
    ....
  19. ncbi request reprint Binders based on dimerised immunoglobulin VH domains
    Jorge Sepulveda
    International Centre for Genetic Engineering and Biotechnology, Molecular Immunology, Area Science Park, Padriciano 99, 34012 Trieste, Italy
    J Mol Biol 333:355-65. 2003
    ....
  20. doi request reprint Using phage display in neurobiology
    Andrew Bradbury
    Los Alamos National Laboratory, Los Alamos, New Mexico, USA
    Curr Protoc Neurosci . 2002
    ..In this unit some of the basic protocols involved in the manipulation of phage display libraries are described, including the rescue and amplification of such libraries, selection and screening from them and testing of derived clones...
  21. pmc Recombinatorial cloning using heterologous lox sites
    Robert W Siegel
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Genome Res 14:1119-29. 2004
    ..This approach will considerably facilitate the high-throughput downstream use of affinity reagents selected by display technologies, as well as being widely applicable to general recombinatorial cloning for genomic purposes...
  22. ncbi request reprint Selection of human antibodies from phage display libraries
    James D Marks
    Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, USA
    Methods Mol Biol 248:161-76. 2004
  23. ncbi request reprint Characterization of the anti-tissue transglutaminase antibody response in nonobese diabetic mice
    Daniele Sblattero
    Department of Biology, University of Trieste, Trieste, Italy
    J Immunol 174:5830-6. 2005
    ..Data from NOD mice on a gluten-free diet suggest that the anti-tTG response is not gluten-dependent. The intestinal Ab response to tTG is a feature of NOD mice, but the underlying mechanisms remain obscure...
  24. ncbi request reprint The cleavage site of C5 from man and animals as a common target for neutralizing human monoclonal antibodies: in vitro and in vivo studies
    Roberto Marzari
    Department of Biology, University of Trieste, Via Fleming 22, I 34127 Trieste, Italy
    Eur J Immunol 32:2773-82. 2002
    ..The scFv TS-A12/22 was tested in a rat model of antigen-induced arthritis and proved to be effective in preventing influx of polymorphonuclear cells into the knee joint and C9 deposition on synovial tissue...

Research Grants2

  1. Species-by-Species Dissection of Microbiomes using Phage Display and Flow Sorting
    Cliff Han; Fiscal Year: 2010
    ..By developing new tools to analyze human microbiomes, we will provide additional methods to study and characterize different bacteria and elucidate their role in human disease. ..
  2. Species-by-Species Dissection of Microbiomes using Phage Display and Flow Sorting
    Andrew Bradbury; Fiscal Year: 2009
    ..By developing new tools to analyze human microbiomes, we will provide additional methods to study and characterize different bacteria and elucidate their role in human disease. ..