Research Topics
| Stephen D DertingerSummaryAffiliation: Litron Laboratories Country: USA Publications
Research Grants
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Detail Information
Publications
Enumeration of micronucleated CD71-positive human reticulocytes with a single-laser flow cytometerStephen D Dertinger
Litron Laboratories, 1351 Mount Hope Avenue, Rochester, NY 14620, USA
Mutat Res 515:3-14. 2002..This high throughput system is potentially important for evaluating the value of the micronucleated reticulocyte endpoint as an index of chromosome breakage and/or chromosome segregational abnormalities in human populations...
In vivo mutation assay based on the endogenous Pig-a locusSteven M Bryce
Litron Laboratories, 200 Canal View Blvd, Rochester, New York 14620, USA
Environ Mol Mutagen 49:256-64. 2008..These data support the hypothesis that an efficient in vivo mutation assay can be developed around flow cytometric enumeration of erythrocytes and/or RETs that exhibit aberrant GPI-anchored protein expression...
Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: dose-response and time-course profiles measured by flow cytometryStephen D Dertinger
Litron Laboratories, Rochester, NY 14623, USA
Mutat Res 634:119-25. 2007....- Integration of mutation and chromosomal damage endpoints into 28-day repeat dose toxicology studiesStephen D Dertinger
Litron Laboratories, Rochester, New York 14623, USA
Toxicol Sci 115:401-11. 2010.... - Flow cytometric scoring of micronucleated erythrocytes: an efficient platform for assessing in vivo cytogenetic damageStephen D Dertinger
Litron Laboratories, 200 Canal View Blvd, Rochester, NY 14623, USA
Mutagenesis 26:139-45. 2011..We conclude with recommendations for additional work that is needed to more fully realise the potential of flow cytometric in vivo MN scoring... - Automated human blood micronucleated reticulocyte measurements for rapid assessment of chromosomal damageStephen D Dertinger
Litron Laboratories, 200 Canal View Boulevard, Rochester, NY, United States
Mutat Res 626:111-9. 2007..Taken together, these data support the hypothesis that the MNCD71+ endpoint represents a valuable biomarker of cytogenetic damage that does not require cell culture or microscopy-based scoring... - When pigs fly: immunomagnetic separation facilitates rapid determination of Pig-a mutant frequency by flow cytometric analysisStephen D Dertinger
Litron Laboratories, Rochester, NY, USA
Mutat Res 721:163-70. 2011..Beyond considerably enhancing the throughput capacity of the analytical platform, these blood-processing procedures were also shown to enhance the precision of the measurements... - International Pig-a gene mutation assay trial: evaluation of transferability across 14 laboratoriesStephen D Dertinger
Litron Laboratories, Rochester, New York, USA
Environ Mol Mutagen 52:690-8. 2011.... - Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: effect of ageStephen D Dertinger
Litron Laboratories, Rochester, NY, USA
Mutat Res 675:77-80. 2009.... - Interlaboratory Pig-a gene mutation assay trial: Studies of 1,3-propane sultone with immunomagnetic enrichment of mutant erythrocytesStephen D Dertinger
Litron Laboratories, Rochester, New York, USA
Environ Mol Mutagen 52:748-55. 2011.... - Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human bloodStephen D Dertinger
Litron Laboratories, Rochester, New York 14620, USA
Environ Mol Mutagen 44:427-35. 2004.... - Micronucleated CD71-positive reticulocytes: a blood-based endpoint of cytogenetic damage in humansStephen D Dertinger
Litron Laboratories, 1351 Mount Hope Avenue, Rochester, NY 14620, USA
Mutat Res 542:77-87. 2003.... - Pig-a mutation: kinetics in rat erythrocytes following exposure to five prototypical mutagensSouk Phonethepswath
Litron Laboratories, Rochester, New York 14623, USA
Toxicol Sci 114:59-70. 2010..The kinetics and ease of scoring this blood-based endpoint suggest that integration into routine toxicology studies will be feasible... - In vivo flow cytometric Pig-a and micronucleus assays: highly sensitive discrimination of the carcinogen/noncarcinogen pair benzo(a)pyrene and pyrene using acute and repeated-dose designsDorothea K Torous
Litron Laboratories, Rochester, NY 14623, USA
Environ Mol Mutagen 53:420-8. 2012.... - Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: I. Intra- and interlaboratory comparison with microscopic scoringStephen D Dertinger
Litron Laboratories, Rochester, New York 14623, USA
Toxicol Sci 94:83-91. 2006..Furthermore, data presented herein demonstrate a clear advantage of flow cytometry-based scoring over microscopy-significantly lower inter- and intralaboratory variation and higher statistical sensitivity... - Comparative scoring of micronucleated reticulocytes in rat peripheral blood by flow cytometry and microscopyDorothea K Torous
Litron Laboratories, 1351 Mount Hope Avenue, Rochester, New York 14620, USA
Toxicol Sci 74:309-14. 2003..These data add further support to the premise that the peripheral blood compartment of rats can be used effectively to detect genotoxicant-induced micronuclei... - Validating high-throughput micronucleus analysis of peripheral reticulocytes for radiation biodosimetry: benchmark against dicentric and CBMN assays in a mouse modelYuhchyau Chen
Department of Radiation Oncology, University of Rochester Medical Center, Rochester, NY 14642, USA
Health Phys 98:218-27. 2010..In mice, the dose range of detection is useful up to 1 Gy (C57Bl/6) and 2 Gy (C3H) but not reliable beyond these dose limits. The utilization of this automated analysis for human radiation biodosimetry is currently under investigation... - Response kinetics of radiation-induced micronucleated reticulocytes in human bone marrow cultureHongliang Sun
Department of Radiation Oncology, University of Rochester Medical Center, 601 Elmwood Ave, Box 647, Rochester, NY 14642 8647, United States
Mutat Res 718:38-43. 2011.... - High content flow cytometric micronucleus scoring method is applicable to attachment cell linesSteven M Bryce
Litron Laboratories, Rochester, New York 14623, USA
Environ Mol Mutagen 51:260-6. 2010..Collectively, these data suggest that flow cytometry is capable of providing reliable micronucleus counts, and that additional information is obtained that appears to discern genotoxic modes of action... - In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicitySteven M Bryce
Litron Laboratories, 200 Canal View Blvd, Rochester, NY, United States
Mutat Res 630:78-91. 2007..Collectively, these results indicate that a comprehensive assessment of genotoxicity and other test article-induced toxicities can be acquired simultaneously using a simple two-color flow cytometry-based technique... - Efficient Monitoring of In Vivo Pig-a Gene Mutation and Chromosomal Damage: Summary of 7 Published Studies and Results From 11 New Reference CompoundsStephen D Dertinger
Litron Laboratories, Rochester, New York 14623
Toxicol Sci 130:328-48. 2012.... - Erythrocyte-based Pig-a gene mutation assay: demonstration of cross-species potentialSouk Phonethepswath
Litron Laboratories, 200 Canal View Boulevard, Rochester, NY, United States
Mutat Res 657:122-6. 2008.... - Gamma-radiation induces micronucleated reticulocytes in 3D bone marrow bioreactors in vitroHongliang Sun
Department of Radiation Oncology, University of Rochester Medical Center, Rochester, NY, United States
Mutat Res 680:49-55. 2009..5-2.0 Gy. The MN-RET response showed a downtrend beyond 2 Gy. Our data suggest that bone marrow culture in the 3D bioreactor may be a useful organ culture system for the investigation of radiation genotoxic effect in vitro... - Miniaturized flow cytometry-based CHO-K1 micronucleus assay discriminates aneugenic and clastogenic modes of actionSteven M Bryce
Litron Laboratories, Rochester, New York 14623, USA
Environ Mol Mutagen 52:280-6. 2011..Taken together, these data indicate that flow cytometry represents an analytical platform that is capable of rapidly and objectively acquiring MN counts while simultaneously providing information on genotoxic MOA... - Miniaturized flow cytometric in vitro micronucleus assay represents an efficient tool for comprehensively characterizing genotoxicity dose-response relationshipsSteven M Bryce
Litron Laboratories, Rochester, NY 14623, United States
Mutat Res 703:191-9. 2010.... - Interlaboratory validation of a CD71-based flow cytometric method (Microflow) for the scoring of micronucleated reticulocytes in mouse peripheral bloodDorothea K Torous
Litron Laboratories, Rochester, New York 14620, USA
Environ Mol Mutagen 45:44-55. 2005..Collectively, data presented in this report suggest that the overall performance of mouse peripheral blood micronucleus tests is enhanced by the use of the flow cytometric scoring procedure... - In vitro micronucleus scoring by flow cytometry: differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliabilitySvetlana L Avlasevich
Litron Laboratories, Rochester, New York, USA
Environ Mol Mutagen 47:56-66. 2006..FCM data were found to correspond closely with microscopy-based measurements. Collectively, these data suggest that this sequential EMA/SYTOX staining procedure provides reliable, high-throughput enumeration of in vitro MN... - Cross-species genetic toxicity assessment accomplished by flow cytometric analysis of bloodJeffrey C Bemis
Litron Laboratories, Rochester, New York, USA
Curr Protoc Toxicol . 2008..This methodology provides a reliable, robust assessment of chromosome damage that is used in basic science research as well as drug-safety screening programs at large pharmaceutical and chemical companies... - Interlaboratory evaluation of a flow cytometric, high content in vitro micronucleus assaySteven M Bryce
Litron Laboratories, Rochester, NY 14623, USA
Mutat Res 650:181-95. 2008..Finally, the data presented herein reinforce concerns about the manner in which cytotoxicity limits are described in guidance documents, since these recommendations tend to cite fixed cut-off values without reference to methodology... - Sublethal radiation injury uncovers a functional transition during erythroid maturationScott A Peslak
Center for Pediatric Biomedical Research, Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USA
Exp Hematol 39:434-45. 2011..To better understand this phenomenon, we investigated the kinetics and apoptotic response of the erythron to sublethal radiation injury... - Practical threshold for micronucleated reticulocyte induction observed for low doses of mitomycin C, Ara-C and colchicineNorihide Asano
Toxicological Research Center, Nitto Denko Corporation, 1 1 2, Shimohozumi, Ibaraki Osaka 567 8680, Japan
Mutagenesis 21:15-20. 2006.... - Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: II. An efficient method of monitoring chromosomal damage in the ratJames T Macgregor
FDA National Center for Toxicological Research, Rockville, Maryland 21012, USA
Toxicol Sci 94:92-107. 2006..The data suggest that flow cytometric assessment of micronucleus induction can be integrated into routine toxicity testing, eliminating the need for a separate bioassay... - In vivo erythrocyte micronucleus assay III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes, with discussion of non-hematopoietic target cells and a single dose-level limit testMakoto Hayashi
Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga Setagaya ku, Tokyo 158 8501, Japan
Mutat Res 627:10-30. 2007..A limit test at a single dose level is currently accepted when toxicity is not dose-limiting... - Quantitative analysis of Howell-Jolly bodies in children with sickle cell diseaseVirginia L Harrod
Department of Hematology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Exp Hematol 35:179-83. 2007..Analysis of these cell populations allows quantitative measurement of splenic filtrative function and possible chromosomal damage... - Flow cytometric analysis of micronuclei in peripheral blood reticulocytes IV: an index of chromosomal damage in the rhesus monkey (Macaca mulatta)Charlotte E Hotchkiss
The Bionetics Corporation, Jefferson, Arkansas 72079, USA
Toxicol Sci 102:352-8. 2008..Microscopy-based scoring is challenging due to the low frequency of RETs and MN-RET in monkeys, but sufficient numbers of cells are easily scored with the flow cytometric procedure... - Flow cytometric analysis of micronuclei in peripheral blood reticulocytes III. An efficient method of monitoring chromosomal damage in the beagle dogSusan B Harper
Department of Veterans Affairs, Washington, DC 20422, USA
Toxicol Sci 100:406-14. 2007..Responses in bone marrow and peripheral blood were highly correlated and similar to or greater than those reported in mice and rats at equitoxic doses... - Elevated frequencies of micronucleated erythrocytes in infants exposed to zidovudine in utero and postpartum to prevent mother-to-child transmission of HIVKristine L Witt
National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
Environ Mol Mutagen 48:322-9. 2007..These results demonstrate that transplacentalZDV exposure is genotoxic in humans. Long-term monitoring of HIV-uninfected ZDV-exposed infants is recommended to ensure their continued health... - Sensitivity of the erythrocyte micronucleus assay: dependence on number of cells scored and inter-animal variabilityGrace E Kissling
National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
Mutat Res 634:235-40. 2007....
Research Grants
- Measuring Cytogenetic Damage in Human BloodStephen Dertinger; Fiscal Year: 2003..abstract_text> ..
- Automation of the vitro Micronucleus AssayStephen Dertinger; Fiscal Year: 2004..abstract_text> ..
- In vivo mutation assay based on pig-a locusStephen Dertinger; Fiscal Year: 2004....
- Versatile Mutation Assay PlatformStephen Dertinger; Fiscal Year: 2007..This research project will optimize and validate a powerful in vitro and in vivo methods for detecting mutagenic agents, thereby enhancing the nation's ability to effectively reduce exposure to these toxic compounds. ..
- High Throughput Radiation BiodosimetryStephen Dertinger; Fiscal Year: 2007..Thus, there is a need for new methodologies with higher throughput. We propose to develop such a radiation biodosimetry assay based on the extremely high analysis rates that are possible with flow cytometry instrumentation. ..
- Rapid Screen for Genotoxicants, Chemoprotectors, and RadioprotectorsStephen Dertinger; Fiscal Year: 2007..In addition, this assay can be used to investigate agents designed to ameliorate the effects of radiation exposure, e.g. biological response modifiers. ..