Stephen D Dertinger

Summary

Affiliation: Litron Laboratories
Country: USA

Publications

  1. ncbi request reprint Enumeration of micronucleated CD71-positive human reticulocytes with a single-laser flow cytometer
    Stephen D Dertinger
    Litron Laboratories, 1351 Mount Hope Avenue, Rochester, NY 14620, USA
    Mutat Res 515:3-14. 2002
  2. doi request reprint In vivo mutation assay based on the endogenous Pig-a locus
    Steven M Bryce
    Litron Laboratories, 200 Canal View Blvd, Rochester, New York 14620, USA
    Environ Mol Mutagen 49:256-64. 2008
  3. pmc Efficient monitoring of in vivo pig-a gene mutation and chromosomal damage: summary of 7 published studies and results from 11 new reference compounds
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York 14623, USA
    Toxicol Sci 130:328-48. 2012
  4. pmc Integration of mutation and chromosomal damage endpoints into 28-day repeat dose toxicology studies
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York 14623, USA
    Toxicol Sci 115:401-11. 2010
  5. doi request reprint Interlaboratory Pig-a gene mutation assay trial: Studies of 1,3-propane sultone with immunomagnetic enrichment of mutant erythrocytes
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York, USA
    Environ Mol Mutagen 52:748-55. 2011
  6. pmc Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: dose-response and time-course profiles measured by flow cytometry
    Stephen D Dertinger
    Litron Laboratories, Rochester, NY 14623, USA
    Mutat Res 634:119-25. 2007
  7. doi request reprint Flow cytometric scoring of micronucleated erythrocytes: an efficient platform for assessing in vivo cytogenetic damage
    Stephen D Dertinger
    Litron Laboratories, 200 Canal View Blvd, Rochester, NY 14623, USA
    Mutagenesis 26:139-45. 2011
  8. pmc When pigs fly: immunomagnetic separation facilitates rapid determination of Pig-a mutant frequency by flow cytometric analysis
    Stephen D Dertinger
    Litron Laboratories, Rochester, NY, USA
    Mutat Res 721:163-70. 2011
  9. doi request reprint International Pig-a gene mutation assay trial: evaluation of transferability across 14 laboratories
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York, USA
    Environ Mol Mutagen 52:690-8. 2011
  10. pmc Automated human blood micronucleated reticulocyte measurements for rapid assessment of chromosomal damage
    Stephen D Dertinger
    Litron Laboratories, 200 Canal View Boulevard, Rochester, NY, United States
    Mutat Res 626:111-9. 2007

Research Grants

  1. Measuring Cytogenetic Damage in Human Blood
    Stephen Dertinger; Fiscal Year: 2003
  2. Automation of the vitro Micronucleus Assay
    Stephen Dertinger; Fiscal Year: 2004
  3. In vivo mutation assay based on pig-a locus
    Stephen Dertinger; Fiscal Year: 2004
  4. Versatile Mutation Assay Platform
    Stephen Dertinger; Fiscal Year: 2007
  5. High Throughput Radiation Biodosimetry
    Stephen Dertinger; Fiscal Year: 2007
  6. Rapid Screen for Genotoxicants, Chemoprotectors, and Radioprotectors
    Stephen Dertinger; Fiscal Year: 2007

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Enumeration of micronucleated CD71-positive human reticulocytes with a single-laser flow cytometer
    Stephen D Dertinger
    Litron Laboratories, 1351 Mount Hope Avenue, Rochester, NY 14620, USA
    Mutat Res 515:3-14. 2002
    ..This high throughput system is potentially important for evaluating the value of the micronucleated reticulocyte endpoint as an index of chromosome breakage and/or chromosome segregational abnormalities in human populations...
  2. doi request reprint In vivo mutation assay based on the endogenous Pig-a locus
    Steven M Bryce
    Litron Laboratories, 200 Canal View Blvd, Rochester, New York 14620, USA
    Environ Mol Mutagen 49:256-64. 2008
    ..These data support the hypothesis that an efficient in vivo mutation assay can be developed around flow cytometric enumeration of erythrocytes and/or RETs that exhibit aberrant GPI-anchored protein expression...
  3. pmc Efficient monitoring of in vivo pig-a gene mutation and chromosomal damage: summary of 7 published studies and results from 11 new reference compounds
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York 14623, USA
    Toxicol Sci 130:328-48. 2012
    ....
  4. pmc Integration of mutation and chromosomal damage endpoints into 28-day repeat dose toxicology studies
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York 14623, USA
    Toxicol Sci 115:401-11. 2010
    ....
  5. doi request reprint Interlaboratory Pig-a gene mutation assay trial: Studies of 1,3-propane sultone with immunomagnetic enrichment of mutant erythrocytes
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York, USA
    Environ Mol Mutagen 52:748-55. 2011
    ....
  6. pmc Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: dose-response and time-course profiles measured by flow cytometry
    Stephen D Dertinger
    Litron Laboratories, Rochester, NY 14623, USA
    Mutat Res 634:119-25. 2007
    ....
  7. doi request reprint Flow cytometric scoring of micronucleated erythrocytes: an efficient platform for assessing in vivo cytogenetic damage
    Stephen D Dertinger
    Litron Laboratories, 200 Canal View Blvd, Rochester, NY 14623, USA
    Mutagenesis 26:139-45. 2011
    ..We conclude with recommendations for additional work that is needed to more fully realise the potential of flow cytometric in vivo MN scoring...
  8. pmc When pigs fly: immunomagnetic separation facilitates rapid determination of Pig-a mutant frequency by flow cytometric analysis
    Stephen D Dertinger
    Litron Laboratories, Rochester, NY, USA
    Mutat Res 721:163-70. 2011
    ..Beyond considerably enhancing the throughput capacity of the analytical platform, these blood-processing procedures were also shown to enhance the precision of the measurements...
  9. doi request reprint International Pig-a gene mutation assay trial: evaluation of transferability across 14 laboratories
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York, USA
    Environ Mol Mutagen 52:690-8. 2011
    ....
  10. pmc Automated human blood micronucleated reticulocyte measurements for rapid assessment of chromosomal damage
    Stephen D Dertinger
    Litron Laboratories, 200 Canal View Boulevard, Rochester, NY, United States
    Mutat Res 626:111-9. 2007
    ..Taken together, these data support the hypothesis that the MNCD71+ endpoint represents a valuable biomarker of cytogenetic damage that does not require cell culture or microscopy-based scoring...
  11. pmc Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: effect of age
    Stephen D Dertinger
    Litron Laboratories, Rochester, NY, USA
    Mutat Res 675:77-80. 2009
    ....
  12. ncbi request reprint Micronucleated CD71-positive reticulocytes: a blood-based endpoint of cytogenetic damage in humans
    Stephen D Dertinger
    Litron Laboratories, 1351 Mount Hope Avenue, Rochester, NY 14620, USA
    Mutat Res 542:77-87. 2003
    ....
  13. ncbi request reprint Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human blood
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York 14620, USA
    Environ Mol Mutagen 44:427-35. 2004
    ....
  14. pmc Pig-a mutation: kinetics in rat erythrocytes following exposure to five prototypical mutagens
    Souk Phonethepswath
    Litron Laboratories, Rochester, New York 14623, USA
    Toxicol Sci 114:59-70. 2010
    ..The kinetics and ease of scoring this blood-based endpoint suggest that integration into routine toxicology studies will be feasible...
  15. doi request reprint Flow cytometric analysis of Pig-a gene mutation and chromosomal damage induced by procarbazine hydrochloride in CD-1 mice
    Souk Phonethepswath
    Litron Laboratories, Rochester, New York
    Environ Mol Mutagen 54:294-8. 2013
    ..Environ. Mol. Mutagen. 54:294-298, 2013. © 2013 Wiley Periodicals, Inc...
  16. doi request reprint In vivo flow cytometric Pig-a and micronucleus assays: highly sensitive discrimination of the carcinogen/noncarcinogen pair benzo(a)pyrene and pyrene using acute and repeated-dose designs
    Dorothea K Torous
    Litron Laboratories, Rochester, NY 14623, USA
    Environ Mol Mutagen 53:420-8. 2012
    ....
  17. ncbi request reprint Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: I. Intra- and interlaboratory comparison with microscopic scoring
    Stephen D Dertinger
    Litron Laboratories, Rochester, New York 14623, USA
    Toxicol Sci 94:83-91. 2006
    ..Furthermore, data presented herein demonstrate a clear advantage of flow cytometry-based scoring over microscopy-significantly lower inter- and intralaboratory variation and higher statistical sensitivity...
  18. ncbi request reprint Comparative scoring of micronucleated reticulocytes in rat peripheral blood by flow cytometry and microscopy
    Dorothea K Torous
    Litron Laboratories, 1351 Mount Hope Avenue, Rochester, New York 14620, USA
    Toxicol Sci 74:309-14. 2003
    ..These data add further support to the premise that the peripheral blood compartment of rats can be used effectively to detect genotoxicant-induced micronuclei...
  19. pmc In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity
    Steven M Bryce
    Litron Laboratories, 200 Canal View Blvd, Rochester, NY, United States
    Mutat Res 630:78-91. 2007
    ..Collectively, these results indicate that a comprehensive assessment of genotoxicity and other test article-induced toxicities can be acquired simultaneously using a simple two-color flow cytometry-based technique...
  20. pmc Response kinetics of radiation-induced micronucleated reticulocytes in human bone marrow culture
    Hongliang Sun
    Department of Radiation Oncology, University of Rochester Medical Center, 601 Elmwood Ave, Box 647, Rochester, NY 14642 8647, United States
    Mutat Res 718:38-43. 2011
    ....
  21. pmc High content flow cytometric micronucleus scoring method is applicable to attachment cell lines
    Steven M Bryce
    Litron Laboratories, Rochester, New York 14623, USA
    Environ Mol Mutagen 51:260-6. 2010
    ..Collectively, these data suggest that flow cytometry is capable of providing reliable micronucleus counts, and that additional information is obtained that appears to discern genotoxic modes of action...
  22. doi request reprint Validating high-throughput micronucleus analysis of peripheral reticulocytes for radiation biodosimetry: benchmark against dicentric and CBMN assays in a mouse model
    Yuhchyau Chen
    Department of Radiation Oncology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Health Phys 98:218-27. 2010
    ..In mice, the dose range of detection is useful up to 1 Gy (C57Bl/6) and 2 Gy (C3H) but not reliable beyond these dose limits. The utilization of this automated analysis for human radiation biodosimetry is currently under investigation...
  23. pmc Gamma-radiation induces micronucleated reticulocytes in 3D bone marrow bioreactors in vitro
    Hongliang Sun
    Department of Radiation Oncology, University of Rochester Medical Center, Rochester, NY, United States
    Mutat Res 680:49-55. 2009
    ..5-2.0 Gy. The MN-RET response showed a downtrend beyond 2 Gy. Our data suggest that bone marrow culture in the 3D bioreactor may be a useful organ culture system for the investigation of radiation genotoxic effect in vitro...
  24. pmc Erythrocyte-based Pig-a gene mutation assay: demonstration of cross-species potential
    Souk Phonethepswath
    Litron Laboratories, 200 Canal View Boulevard, Rochester, NY, United States
    Mutat Res 657:122-6. 2008
    ....
  25. doi request reprint Flow cytometric 96-well microplate-based in vitro micronucleus assay with human TK6 cells: protocol optimization and transferability assessment
    Steven M Bryce
    Litron Laboratories, Rochester, New York 14623, USA
    Environ Mol Mutagen 54:180-94. 2013
    ..The 96-well format consumes considerably less compound than conventional in vitro MN test methods, and the high information content provided by flow cytometry helps guard against irrelevant positive results arising from overt toxicity...
  26. doi request reprint Miniaturized flow cytometry-based CHO-K1 micronucleus assay discriminates aneugenic and clastogenic modes of action
    Steven M Bryce
    Litron Laboratories, Rochester, New York 14623, USA
    Environ Mol Mutagen 52:280-6. 2011
    ..Taken together, these data indicate that flow cytometry represents an analytical platform that is capable of rapidly and objectively acquiring MN counts while simultaneously providing information on genotoxic MOA...
  27. pmc Miniaturized flow cytometric in vitro micronucleus assay represents an efficient tool for comprehensively characterizing genotoxicity dose-response relationships
    Steven M Bryce
    Litron Laboratories, Rochester, NY 14623, United States
    Mutat Res 703:191-9. 2010
    ....
  28. ncbi request reprint Interlaboratory validation of a CD71-based flow cytometric method (Microflow) for the scoring of micronucleated reticulocytes in mouse peripheral blood
    Dorothea K Torous
    Litron Laboratories, Rochester, New York 14620, USA
    Environ Mol Mutagen 45:44-55. 2005
    ..Collectively, data presented in this report suggest that the overall performance of mouse peripheral blood micronucleus tests is enhanced by the use of the flow cytometric scoring procedure...
  29. ncbi request reprint In vitro micronucleus scoring by flow cytometry: differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability
    Svetlana L Avlasevich
    Litron Laboratories, Rochester, New York, USA
    Environ Mol Mutagen 47:56-66. 2006
    ..FCM data were found to correspond closely with microscopy-based measurements. Collectively, these data suggest that this sequential EMA/SYTOX staining procedure provides reliable, high-throughput enumeration of in vitro MN...
  30. pmc Interlaboratory evaluation of a flow cytometric, high content in vitro micronucleus assay
    Steven M Bryce
    Litron Laboratories, Rochester, NY 14623, USA
    Mutat Res 650:181-95. 2008
    ..Finally, the data presented herein reinforce concerns about the manner in which cytotoxicity limits are described in guidance documents, since these recommendations tend to cite fixed cut-off values without reference to methodology...
  31. doi request reprint Cross-species genetic toxicity assessment accomplished by flow cytometric analysis of blood
    Jeffrey C Bemis
    Litron Laboratories, Rochester, New York, USA
    Curr Protoc Toxicol . 2008
    ..This methodology provides a reliable, robust assessment of chromosome damage that is used in basic science research as well as drug-safety screening programs at large pharmaceutical and chemical companies...
  32. pmc Sublethal radiation injury uncovers a functional transition during erythroid maturation
    Scott A Peslak
    Center for Pediatric Biomedical Research, Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USA
    Exp Hematol 39:434-45. 2011
    ..To better understand this phenomenon, we investigated the kinetics and apoptotic response of the erythron to sublethal radiation injury...
  33. doi request reprint Erythrocyte-Based Pig-a Gene Mutation Assay
    Jeffrey C Bemis
    Litron Laboratories, Rochester, NY, USA
    Methods Mol Biol 1044:51-77. 2013
    ..This unit describes a procedure for the collection, processing, and analysis of peripheral blood samples using materials supplied in MutaFlow(®) kits and a common benchtop flow cytometer. ..
  34. ncbi request reprint Quantitative analysis of Howell-Jolly bodies in children with sickle cell disease
    Virginia L Harrod
    Department of Hematology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Exp Hematol 35:179-83. 2007
    ..Analysis of these cell populations allows quantitative measurement of splenic filtrative function and possible chromosomal damage...
  35. ncbi request reprint In vivo erythrocyte micronucleus assay III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes, with discussion of non-hematopoietic target cells and a single dose-level limit test
    Makoto Hayashi
    Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga Setagaya ku, Tokyo 158 8501, Japan
    Mutat Res 627:10-30. 2007
    ..A limit test at a single dose level is currently accepted when toxicity is not dose-limiting...
  36. ncbi request reprint Flow cytometric analysis of micronuclei in peripheral blood reticulocytes III. An efficient method of monitoring chromosomal damage in the beagle dog
    Susan B Harper
    Department of Veterans Affairs, Washington, DC 20422, USA
    Toxicol Sci 100:406-14. 2007
    ..Responses in bone marrow and peripheral blood were highly correlated and similar to or greater than those reported in mice and rats at equitoxic doses...
  37. ncbi request reprint Flow cytometric analysis of micronuclei in peripheral blood reticulocytes: II. An efficient method of monitoring chromosomal damage in the rat
    James T Macgregor
    FDA National Center for Toxicological Research, Rockville, Maryland 21012, USA
    Toxicol Sci 94:92-107. 2006
    ..The data suggest that flow cytometric assessment of micronucleus induction can be integrated into routine toxicity testing, eliminating the need for a separate bioassay...
  38. ncbi request reprint Practical threshold for micronucleated reticulocyte induction observed for low doses of mitomycin C, Ara-C and colchicine
    Norihide Asano
    Toxicological Research Center, Nitto Denko Corporation, 1 1 2, Shimohozumi, Ibaraki Osaka 567 8680, Japan
    Mutagenesis 21:15-20. 2006
    ....
  39. pmc Sensitivity of the erythrocyte micronucleus assay: dependence on number of cells scored and inter-animal variability
    Grace E Kissling
    National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Mutat Res 634:235-40. 2007
    ....
  40. doi request reprint Flow cytometric analysis of micronuclei in peripheral blood reticulocytes IV: an index of chromosomal damage in the rhesus monkey (Macaca mulatta)
    Charlotte E Hotchkiss
    The Bionetics Corporation, Jefferson, Arkansas 72079, USA
    Toxicol Sci 102:352-8. 2008
    ..Microscopy-based scoring is challenging due to the low frequency of RETs and MN-RET in monkeys, but sufficient numbers of cells are easily scored with the flow cytometric procedure...
  41. pmc Elevated frequencies of micronucleated erythrocytes in infants exposed to zidovudine in utero and postpartum to prevent mother-to-child transmission of HIV
    Kristine L Witt
    National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    Environ Mol Mutagen 48:322-9. 2007
    ..These results demonstrate that transplacentalZDV exposure is genotoxic in humans. Long-term monitoring of HIV-uninfected ZDV-exposed infants is recommended to ensure their continued health...

Research Grants8

  1. Measuring Cytogenetic Damage in Human Blood
    Stephen Dertinger; Fiscal Year: 2003
    ..abstract_text> ..
  2. Automation of the vitro Micronucleus Assay
    Stephen Dertinger; Fiscal Year: 2004
    ..abstract_text> ..
  3. In vivo mutation assay based on pig-a locus
    Stephen Dertinger; Fiscal Year: 2004
    ....
  4. Versatile Mutation Assay Platform
    Stephen Dertinger; Fiscal Year: 2007
    ..This research project will optimize and validate a powerful in vitro and in vivo methods for detecting mutagenic agents, thereby enhancing the nation's ability to effectively reduce exposure to these toxic compounds. ..
  5. High Throughput Radiation Biodosimetry
    Stephen Dertinger; Fiscal Year: 2007
    ..Thus, there is a need for new methodologies with higher throughput. We propose to develop such a radiation biodosimetry assay based on the extremely high analysis rates that are possible with flow cytometry instrumentation. ..
  6. Rapid Screen for Genotoxicants, Chemoprotectors, and Radioprotectors
    Stephen Dertinger; Fiscal Year: 2007
    ..In addition, this assay can be used to investigate agents designed to ameliorate the effects of radiation exposure, e.g. biological response modifiers. ..