LYNNE C CASSIMERIS

Summary

Affiliation: Lehigh University
Country: USA

Publications

  1. pmc Specific in vivo labeling of tyrosinated α-tubulin and measurement of microtubule dynamics using a GFP tagged, cytoplasmically expressed recombinant antibody
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, United States of America
    PLoS ONE 8:e59812. 2013
  2. doi request reprint Fueled by microtubules: does tubulin dimer/polymer partitioning regulate intracellular metabolism?
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Cytoskeleton (Hoboken) 69:133-43. 2012
  3. pmc Gene expression profiles in mouse embryo fibroblasts lacking stathmin, a microtubule regulatory protein, reveal changes in the expression of genes contributing to cell motility
    Danielle N Ringhoff
    Chemistry Department, Lehigh University, Bethlehem, PA 18015, USA
    BMC Genomics 10:343. 2009
  4. doi request reprint TOGp regulates microtubule assembly and density during mitosis and contributes to chromosome directional instability
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, USA
    Cell Motil Cytoskeleton 66:535-45. 2009
  5. doi request reprint Microtubule assembly: lattice GTP to the rescue
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, PA 18017, USA
    Curr Biol 19:R174-6. 2009
  6. ncbi request reprint Tubulin delivery: polymerization chaperones for microtubule assembly?
    Lynne Cassimeris
    Department of Biological Sciences, 111 Research Drive, Lehigh University, Bethlehem, PA 18015, USA
    Dev Cell 13:455-6. 2007
  7. ncbi request reprint Mitosis: riding the protofilament curl
    Lynne Cassimeris
    Department of Biological Sciences, 111 Research Drive, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Curr Biol 16:R214-6. 2006
  8. pmc TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Mol Biol Cell 15:1580-90. 2004
  9. ncbi request reprint Accessory protein regulation of microtubule dynamics throughout the cell cycle
    L Cassimeris
    Department of Biological Sciences 111 Research Drive Lehigh University Bethlehem PA 18015 USA
    Curr Opin Cell Biol 11:134-41. 1999
  10. ncbi request reprint XMAP215 is a long thin molecule that does not increase microtubule stiffness
    L Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA
    J Cell Sci 114:3025-33. 2001

Research Grants

Collaborators

Detail Information

Publications25

  1. pmc Specific in vivo labeling of tyrosinated α-tubulin and measurement of microtubule dynamics using a GFP tagged, cytoplasmically expressed recombinant antibody
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, United States of America
    PLoS ONE 8:e59812. 2013
    ..Thus, a recombinant antibody with weak affinity for its substrate can be used as a specific intracellular biosensor that can differentiate between unmodified and post-translationally modified forms of a protein...
  2. doi request reprint Fueled by microtubules: does tubulin dimer/polymer partitioning regulate intracellular metabolism?
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Cytoskeleton (Hoboken) 69:133-43. 2012
    ....
  3. pmc Gene expression profiles in mouse embryo fibroblasts lacking stathmin, a microtubule regulatory protein, reveal changes in the expression of genes contributing to cell motility
    Danielle N Ringhoff
    Chemistry Department, Lehigh University, Bethlehem, PA 18015, USA
    BMC Genomics 10:343. 2009
    ....
  4. doi request reprint TOGp regulates microtubule assembly and density during mitosis and contributes to chromosome directional instability
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, USA
    Cell Motil Cytoskeleton 66:535-45. 2009
    ..We hypothesize that TOGp is required for chromosome motility as a downstream consequence of reduced microtubule dynamics and/or density. Cell Motil. Cytoskeleton 2009. (c) 2009 Wiley-Liss, Inc...
  5. doi request reprint Microtubule assembly: lattice GTP to the rescue
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, PA 18017, USA
    Curr Biol 19:R174-6. 2009
    ..These GTP-tubulin islands may act as stop signs or speed bumps, switching a shortening microtubule back into a growing state...
  6. ncbi request reprint Tubulin delivery: polymerization chaperones for microtubule assembly?
    Lynne Cassimeris
    Department of Biological Sciences, 111 Research Drive, Lehigh University, Bethlehem, PA 18015, USA
    Dev Cell 13:455-6. 2007
    ....
  7. ncbi request reprint Mitosis: riding the protofilament curl
    Lynne Cassimeris
    Department of Biological Sciences, 111 Research Drive, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Curr Biol 16:R214-6. 2006
    ..New studies measure the force produced by depolymerization and show that protein ring complexes can couple depolymerization to movement. These results have implications for anaphase chromosome motility and mitotic evolution...
  8. pmc TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly
    Lynne Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Mol Biol Cell 15:1580-90. 2004
    ..The major functions of TOGp during mitosis are to focus MT minus ends at spindle poles, maintain centrosome integrity, and contribute to spindle bipolarity...
  9. ncbi request reprint Accessory protein regulation of microtubule dynamics throughout the cell cycle
    L Cassimeris
    Department of Biological Sciences 111 Research Drive Lehigh University Bethlehem PA 18015 USA
    Curr Opin Cell Biol 11:134-41. 1999
    ..In general, although some microtubule-stabilizing proteins are inactivated at entry into mitosis, a critical balance between microtubule stabilizers and destabilizers is necessary for assembly of the mitotic spindle...
  10. ncbi request reprint XMAP215 is a long thin molecule that does not increase microtubule stiffness
    L Cassimeris
    Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA
    J Cell Sci 114:3025-33. 2001
    ..Finally, sequence analysis shows that the N-terminal half of XMAP215 contains four repeats, each composed of multiple HEAT repeats...
  11. ncbi request reprint The oncoprotein 18/stathmin family of microtubule destabilizers
    Lynne Cassimeris
    Department of Biological Sciences, 111 Research Drive, Lehigh University, Bethlehem, PA18015, USA
    Curr Opin Cell Biol 14:18-24. 2002
    ..A spatial gradient of inactive Op18/stathmin associated with chromatin or microtubules could contribute significantly to mitotic spindle assembly...
  12. ncbi request reprint Regulated assembly of the mitotic spindle: a perspective from two ends
    Lynne Cassimeris
    Dept of Biological Sciences, 111 Research Dr, Lehigh University, Bethlehem, PA 18015, USA
    Curr Issues Mol Biol 5:99-112. 2003
    ..In this review, we discuss current models of how centrosomes and chromosomes organize the spindle for their equal distribution to each daughter cell...
  13. pmc Stathmin regulates centrosomal nucleation of microtubules and tubulin dimer/polymer partitioning
    Danielle N Ringhoff
    Department of Chemistry and Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA
    Mol Biol Cell 20:3451-8. 2009
    ..These data support a model in which stathmin functions in interphase to control the partitioning of tubulins between dimer and polymer pools by setting the number of microtubules per cell...
  14. pmc Mechanochemical model of microtubule structure and self-assembly kinetics
    Vincent VanBuren
    Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA
    Biophys J 89:2911-26. 2005
    ..Finally, simulations of the binding and structural effects of XMAP215 produced the experimentally observed growth and shortening rates, and tip structure...
  15. ncbi request reprint The contributions of microtubule stability and dynamic instability to adenovirus nuclear localization efficiency
    James C Warren
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Cell Motil Cytoskeleton 64:675-89. 2007
    ..Because Rac1 mediates persistent growth of microtubules to the lamellar regions of cells, we propose that Ad-induced activation of Rac1 enhances the ability of microtubules to "search and capture" incoming virus particles...
  16. pmc Estimates of lateral and longitudinal bond energies within the microtubule lattice
    Vincent VanBuren
    Department of Biological Sciences, Lehigh University, 111 Research Drive, Bethlehem, PA 18015, USA
    Proc Natl Acad Sci U S A 99:6035-40. 2002
    ..Our model serves as a platform to examine GTP-cap dynamics and allows predictions of how MT-associated proteins and other effectors alter the energetics of MT assembly...
  17. pmc Organization and dynamics of growing microtubule plus ends during early mitosis
    Michelle Piehl
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Mol Biol Cell 14:916-25. 2003
    ..Both dynein and microtubules would then be in a position to participate in nuclear envelope breakdown, as described in recent studies...
  18. pmc Positional analyses of BRCA1-dependent expression in Saccharomyces cerevisiae
    Robert V Skibbens
    Lehigh University, Department of Biological Sciences, Bethlehem, Pennsylvania 18015, USA
    Cell Cycle 7:3928-34. 2008
    ..Cluster-functional analysis reveals that these repressed factors are required for mitotic stability and provide a novel molecular explanation for the conditional lethality observed between BRCA1 and chromosome segregation genes...
  19. pmc Centrosome maturation: measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1
    Michelle Piehl
    Department of Biological Sciences, Lehigh University, 111 Research Drive, Bethlehem, PA 18015, USA
    Proc Natl Acad Sci U S A 101:1584-8. 2004
    ....
  20. pmc Infection with replication-deficient adenovirus induces changes in the dynamic instability of host cell microtubules
    James C Warren
    Department of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA
    Mol Biol Cell 17:3557-68. 2006
    ..These results demonstrate that adenovirus infection induces a significant stabilizing effect on host cell microtubule dynamics, which involve, but are not limited to, the activation of the RhoGTPases RhoA and Rac1...
  21. ncbi request reprint Cytoskeleton: microtubules born on the run
    Bret E Becker
    Department of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA
    Curr Biol 15:R551-4. 2005
    ..Recent work in the fission yeast describes a novel mechanism for microtubule self-organization in the absence of centrosomes; this mechanism may function in a variety of cell types found in diverse organisms...
  22. ncbi request reprint Cell division: eg'ing on microtubule flux
    Lynne Cassimeris
    Department of Biological Sciences, 111 Research Drive, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Curr Biol 14:R1000-2. 2004
    ..Mitotic microtubules are moved toward spindle poles in a process known as flux. Several proteins responsible for flux have recently been identified, significantly advancing our understanding of chromosome movements in mitosis...
  23. ncbi request reprint Stathmin/oncoprotein 18, a microtubule regulatory protein, is required for survival of both normal and cancer cell lines lacking the tumor suppressor, p53
    Bruce K Carney
    Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA
    Cancer Biol Ther 9:699-709. 2010
    ..Our results demonstrate that stathmin is required for cell survival in cells lacking p53, suggesting that stathmin depletion could be used therapeutically to induce apoptosis in tumors without functional p53...
  24. pmc Human EFO1p exhibits acetyltransferase activity and is a unique combination of linker histone and Ctf7p/Eco1p chromatid cohesion establishment domains
    Aaron M Bellows
    Department of Biological Sciences, Lehigh University, 111 Research Drive, Bethlehem, PA 18015, USA
    Nucleic Acids Res 31:6334-43. 2003
    ..These findings document the first characterization of a novel human acetyltransferase, hEFO1p, that is comprised of both linker histone and Ctf7p/Eco1p domains...
  25. pmc Cellular entry and nuclear targeting by a highly anionic molecular umbrella
    Mohamed Mehiri
    Departments of Chemistry and Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Bioconjug Chem 19:1510-3. 2008
    ..The fact that 1 is concentrated at the nucleus raises the possibility that molecular umbrellas of this type could be used for the nuclear targeting of drugs...

Research Grants8

  1. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2002
    ..Changes in microtubule turnover will be measured by tubulin fluorescence photoactivation and redistribution. ..
  2. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..