Research Topics
Genomes and Genes | Hong Wei WangSummaryAffiliation: Lawrence Berkeley National Laboratory Country: USA Publications
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Publications
Architecture of the yeast Rrp44 exosome complex suggests routes of RNA recruitment for 3' end processingHong Wei Wang
Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 104:16844-9. 2007..The Rrp44-exosome (RE) architecture suggests an active site sequestration mechanism for strict control of 3' exoribonuclease activity in the RE complex...
Assembly of GMPCPP-bound tubulin into helical ribbons and tubes and effect of colchicineHong Wei Wang
Life Science Division, Lawrence Berkeley National Lab, Berkeley, California, USA
Cell Cycle 4:1157-60. 2005..Finally, we add our thoughts on the possible biological meaning of tubulin polymerization versatility...- Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complexStefan Westermann
Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
Mol Cell 17:277-90. 2005..These observations suggest a mechanism by which the kinetochore can recognize and stay attached to the plus ends of microtubules... - The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule endsStefan Westermann
Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 94720 3202, USA
Nature 440:565-9. 2006..The Dam1 ring complex is a molecular device that can translate the force generated by microtubule depolymerization into movement along the lattice to facilitate chromosome segregation... - Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanismsHong Wei Wang
Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, California 94720, USA
Nat Struct Mol Biol 14:721-6. 2007..One of Ipl1's roles might be to fine-tune the coupling of the microtubule interaction with the conformational change required for oligomerization, with phosphorylation resulting in ring breakdown... - Architecture and flexibility of the yeast Ndc80 kinetochore complexHong Wei Wang
Life Science Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
J Mol Biol 383:894-903. 2008..We hypothesize that the kink in Ndc80 is essential for correct kinetochore geometry and could be part of a tension-sensing mechanism at the kinetochore... - The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubuleVincent H Ramey
Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA
Mol Biol Cell 22:457-66. 2011..The present studies constitute a significant step forward by linking structural and biochemical observations toward a comprehensive understanding of the Dam1 complex... - Nucleotide-dependent bending flexibility of tubulin regulates microtubule assemblyHong Wei Wang
Howard Hughes Medical Institute, University of California Berkeley, Berkeley, California 94720 3200, USA
Nature 435:911-5. 2005..Closure into a microtubule does not require GTP hydrolysis... 
Cryo-EM studies of microtubule structural intermediates and kinetochore-microtubule interactionsEva Nogales
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3220, USA
Methods Cell Biol 95:129-56. 2010..We describe the experimental in vitro protocols to produce biologically relevant self-assembled structures of Dam 1 around microtubules and their structural characterization by cryo-EM...- Tubulin rings: which way do they curve?Eva Nogales
Howard Hughes Medical Institute, Molecular and Cell Biology Department and Life Science Division, Lawrence Berkeley National Laboratory, 355 Life Sciences Addition, University of California, 94720 3200, USA
Curr Opin Struct Biol 13:256-61. 2003..How these kinks relate to what happens when the stored energy of GTP hydrolysis is released, freeing GDP*tubulin into an unconstrained state, remains an open question... - Structural intermediates in microtubule assembly and disassembly: how and why?Eva Nogales
Howard Hughes Medical Institute, Molecular and Cell Biology Department, UC Berkeley and Lawrence Berkeley National Lab, 355 LSA UC Berkeley, Berkeley, CA 94720 3200, USA
Curr Opin Cell Biol 18:179-84. 2006..The discovery of these intermediates a decade ago has been followed recently by quantitative descriptions of their structure and their relationship to nucleotide state... - Structural mechanisms underlying nucleotide-dependent self-assembly of tubulin and its relativesEva Nogales
Howard Hughes Medical Institute, Molecular and Cell Biology Department, University of California Berkeley and Lawrence Berkeley National Laboratory, 355 LSA, University of California Berkeley, Berkeley, CA 94720 3200, USA
Curr Opin Struct Biol 16:221-9. 2006..In particular, a two-state mechanism has been proposed for the recycling of alphabeta-tubulin during the microtubule disassembly-assembly cycle; this mechanism may be unique to eukaryotic dimeric tubulin and the microtubule structure... - Ab initio reconstruction of helical samples with heterogeneity, disorder and coexisting symmetriesVincent H Ramey
Biophysics Graduate Program, UC Berkeley, Berkeley, CA, USA
J Struct Biol 167:97-105. 2009.... - Structural insights into RNA processing by the human RISC-loading complexHong Wei Wang
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
Nat Struct Mol Biol 16:1148-53. 2009..A model generated by docking the available atomic structures of Dicer and Argonaute homologs into the RLC reconstruction suggests a mechanism for siRNA transfer from Dicer to AGO2... - An iterative Fourier-Bessel algorithm for reconstruction of helical structures with severe Bessel overlapHong-Wei Wang
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
J Struct Biol 149:65-78. 2005..The algorithm has also been applied with very satisfactory results to the reconstruction of 13-protofilament microtubules, which is another helical structure that suffer Bessel overlap, suggesting its generality... - [Effect of N-terminal deletion on biological activity of vascular endothelial cell growth inhibitor]Min Zhang
Department of Microbiology, Second Military Medical University, Shanghai 200433, China
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:133-7. 2003..These results suggest that the first 43 amino acids from N-terminus of VEGI have no significant effect on biological activity, but the amino acids 44-51 at N-terminus are required for full biological activity... - [Enhancement by an in vivo-activated promoter of immunogenicity of recombinant attenuated Salmonella typhimurium expressing hepatitis C virus core antigen]Ping Zhao
Department of Microbiology, Second Military Medical University, Shanghai 200433, China
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:266-70. 2003.... - Ring-like pore structures of SecA: implication for bacterial protein-conducting channelsHong Wei Wang
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembranes, Tsinghua University, Beijing 100084, China
Proc Natl Acad Sci U S A 100:4221-6. 2003..They do not form in solution or in the presence of uncharged phosphatidylcholine. These ring-like phospholipid-induced pore-structures may form the core of bacterial protein-conducting channels through bacterial membranes... - Two-dimensional crystallization of a small heat shock protein HSP16.3 on lipid layerYong Chen
Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembrane, Tsinghua University, Beijing 100084, PR China
Biochem Biophys Res Commun 310:360-6. 2003..These results provide a basis for further investigation on the unique dimeric structure of HSP16.3 and its functions in vivo... - Polymorphism of structural forms of C-reactive proteinHong-Wei Wang
Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembranes, Tsinghua University, Beijing 100084, P.R. China
Int J Mol Med 9:665-71. 2002..The different structural forms may convert to each other under certain conditions, which gives some hints as to the structural basis of multiple functions of CRP...