Cheryl A Kerfeld


Affiliation: Lawrence Berkeley National Laboratory
Country: USA


  1. Dominguez Martin M, Kerfeld C. Engineering the orange carotenoid protein for applications in synthetic biology. Curr Opin Struct Biol. 2019;57:110-117 pubmed publisher
    ..Here based on recent foundational results, we describe potential ways that the OCP can be engineered to serve a wide range of applications in synthetic biology. ..
  2. Dominguez Martin M, Polivka T, Sutter M, Ferlez B, Lechno Yossef S, Montgomery B, et al. Structural and spectroscopic characterization of HCP2. Biochim Biophys Acta Bioenerg. 2019;: pubmed publisher
  3. Greber B, Sutter M, Kerfeld C. The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly. Structure. 2019;: pubmed publisher
    ..We discuss the implications of these findings on shell assembly and function. ..
  4. Ferlez B, Sutter M, Kerfeld C. Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles. MBio. 2019;10: pubmed publisher
    ..In this review, we will focus on the diversity, function, and physiological importance of GRMs, with particular attention given to their associated and enigmatic redox proteins. ..
  5. Sommer M, Sutter M, Gupta S, Kirst H, Turmo A, Lechno Yossef S, et al. Heterohexamers Formed by CcmK3 and CcmK4 Increase the Complexity of Beta Carboxysome Shells. Plant Physiol. 2019;179:156-167 pubmed publisher
    ..Because β-carboxysomes are obligately expressed, heterohexamer formation and capping could provide a rapid and reversible means to alter metabolite flux across the shell in response to environmental/growth conditions. ..
  6. Gaudana S, Zarzycki J, Moparthi V, Kerfeld C. Bioinformatic analysis of the distribution of inorganic carbon transporters and prospective targets for bioengineering to increase Ci uptake by cyanobacteria. Photosynth Res. 2015;126:99-109 pubmed publisher
  7. Cai F, Dou Z, Bernstein S, Leverenz R, Williams E, Heinhorst S, et al. Advances in Understanding Carboxysome Assembly in Prochlorococcus and Synechococcus Implicate CsoS2 as a Critical Component. Life (Basel). 2015;5:1141-71 pubmed publisher
    ..Analogies between the pathway for β-carboxysome biogenesis and our model for α-carboxysome assembly are discussed. ..
  8. LASSILA J, Bernstein S, Kinney J, Axen S, Kerfeld C. Assembly of robust bacterial microcompartment shells using building blocks from an organelle of unknown function. J Mol Biol. 2014;426:2217-28 pubmed publisher
    ..Our results demonstrate the value of selecting from the diversity of BMC shell building blocks found in genomic sequence data for the construction of novel compartments. ..
  9. Hagen A, Sutter M, Sloan N, Kerfeld C. Programmed loading and rapid purification of engineered bacterial microcompartment shells. Nat Commun. 2018;9:2881 pubmed publisher

More Information


  1. Zarzycki J, Erbilgin O, Kerfeld C. Bioinformatic characterization of glycyl radical enzyme-associated bacterial microcompartments. Appl Environ Microbiol. 2015;81:8315-29 pubmed publisher
    ..Understanding this plasticity of function within a single BMC family, including characterization of differences in permeability and assembly, can inform approaches to BMC bioengineering and the design of therapeutics. ..
  2. Gupta S, Guttman M, Leverenz R, Zhumadilova K, Pawlowski E, Petzold C, et al. Local and global structural drivers for the photoactivation of the orange carotenoid protein. Proc Natl Acad Sci U S A. 2015;112:E5567-74 pubmed publisher
    ..Collectively, these data provide experimental evidence for an ensemble of local and global structural changes, upon activation of the OCP, that are essential for photoprotection. ..
  3. Gonzalez Esquer C, Shubitowski T, Kerfeld C. Streamlined Construction of the Cyanobacterial CO2-Fixing Organelle via Protein Domain Fusions for Use in Plant Synthetic Biology. Plant Cell. 2015;27:2637-44 pubmed publisher
  4. Leverenz R, Sutter M, Wilson A, Gupta S, Thurotte A, Bourcier de Carbon C, et al. PHOTOSYNTHESIS. A 12 Ã… carotenoid translocation in a photoswitch associated with cyanobacterial photoprotection. Science. 2015;348:1463-6 pubmed publisher
    ..Our results identify the origin of the photochromic changes in the OCP triggered by light and reveal the structural determinants required for interaction with the light-harvesting antenna during photoprotection. ..
  5. Kerfeld C, Erbilgin O. Bacterial microcompartments and the modular construction of microbial metabolism. Trends Microbiol. 2015;23:22-34 pubmed publisher
    ..Viewing BMCs as genetic, structural, functional, and evolutionary modules provides a framework for understanding both BMC-mediated metabolism and for adapting their architectures for applications in synthetic biology. ..
  6. Cai F, Bernstein S, Wilson S, Kerfeld C. Production and Characterization of Synthetic Carboxysome Shells with Incorporated Luminal Proteins. Plant Physiol. 2016;170:1868-77 pubmed publisher
    ..This system will be especially useful for developing synthetic carboxysomes for plant engineering. ..
  7. Kerfeld C, Melnicki M. Assembly, function and evolution of cyanobacterial carboxysomes. Curr Opin Plant Biol. 2016;31:66-75 pubmed publisher
  8. Montgomery B, Lechno Yossef S, Kerfeld C. Interrelated modules in cyanobacterial photosynthesis: the carbon-concentrating mechanism, photorespiration, and light perception. J Exp Bot. 2016;67:2931-40 pubmed publisher
    ..We probe this connection by investigating light inputs into the CCM and photorespiratory pathways in the chromatically acclimating cyanobacterium Fremyella diplosiphon. ..
  9. Gonzalez Esquer C, Smarda J, Rippka R, Axen S, Guglielmi G, Gugger M, et al. Cyanobacterial ultrastructure in light of genomic sequence data. Photosynth Res. 2016;129:147-57 pubmed publisher
    ..This compilation of images and genome-level domain occurrence will prove useful for a variety of analyses of cyanobacterial sequence data and provides a guidebook to morphological features. ..
  10. Melnicki M, Leverenz R, Sutter M, López Igual R, Wilson A, Pawlowski E, et al. Structure, Diversity, and Evolution of a New Family of Soluble Carotenoid-Binding Proteins in Cyanobacteria. Mol Plant. 2016;9:1379-1394 pubmed publisher
    ..Our results demonstrate that the HCPs are a new family of functionally diverse carotenoid-binding proteins found among ecophysiologically diverse cyanobacteria. ..
  11. Bao H, Melnicki M, Kerfeld C. Structure and functions of Orange Carotenoid Protein homologs in cyanobacteria. Curr Opin Plant Biol. 2017;37:1-9 pubmed publisher
    ..Distinct functions from the canonical OCP have been revealed for some of these paralogs by recent structural and functional studies. ..
  12. Sutter M, Greber B, Aussignargues C, Kerfeld C. Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell. Science. 2017;356:1293-1297 pubmed publisher
    ..Given the conservation among shell proteins of all bacterial microcompartments, these principles apply to functionally diverse organelles and can inform the design and engineering of shells with new functionalities. ..
  13. Plegaria J, Kerfeld C. Engineering nanoreactors using bacterial microcompartment architectures. Curr Opin Biotechnol. 2018;51:1-7 pubmed publisher
  14. Zarzycki J, Kerfeld C. The crystal structures of the tri-functional Chloroflexus aurantiacus and bi-functional Rhodobacter sphaeroides malyl-CoA lyases and comparison with CitE-like superfamily enzymes and malate synthases. BMC Struct Biol. 2013;13:28 pubmed publisher
    ..Further studies involving site-directed mutagenesis based on these structures may be required to solve this puzzling question. ..