Research Topics
| Jay GrovesSummaryAffiliation: Lawrence Berkeley National Laboratory Country: USA Publications
Research Grants
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Detail Information
Publications
Electrical manipulation of supported lipid membranes by embedded electrodesBryan L Jackson
Department of Chemistry, University of California, Berkeley, California 94720, USA
Langmuir 24:6189-93. 2008..Application of lower magnitude direct current fields resulted in electrophoretic redistribution of the membrane probes and electro-osmotic reorganization of membrane associated proteins...
Engineering supported membranes for cell biologyCheng Han Yu
Research Centre of Excellence in Mechanobiology, National University of Singapore, Singapore
Med Biol Eng Comput 48:955-63. 2010..The integration of biological components into synthetic devices provides a unique approach to investigate molecular mechanisms in cell biology...- Cell membrane array fabrication and assay technologyVictoria Yamazaki
Synamem Corporation, 863 Mitten Road, Suite 101, Burlingame, CA 94010, USA
BMC Biotechnol 5:18. 2005..By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processes has been considerably more difficult... 
Learning the chemical language of cell-surface interactionsJay T Groves
Department of Chemistry, University of California, Berkeley, USA
Sci STKE 2005:pe45. 2005..A number of promising advances in the development of synthetic interfaces with cells suggest that the move to predictive design is under way...- Spatial mutation of the T cell immunological synapseJay T Groves
Department of Chemistry, University of California Berkeley, Berkeley, CA 94720, USA
Curr Opin Chem Biol 10:544-50. 2006..These methodologies complement conventional genetic and pharmacological approaches, both of which are chemical in nature, by perturbing the system through exclusively physical mechanisms... - Fluorescence imaging of membrane dynamicsJay T Groves
Department of Chemistry, University of California, Berkeley, CA 94720, USA
Annu Rev Biomed Eng 10:311-38. 2008..For each, we discuss the physics underpinning the approach, its practical implementation, and recent examples highlighting its achievements in exploring the membrane environment... - Supported planar bilayers in studies on immune cell adhesion and communicationJay T Groves
Department of Chemistry and Physical Biosciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
J Immunol Methods 278:19-32. 2003..Supported planar bilayers are simple to form by liposome fusion and recent advances in micro- and nanotechnology greatly extend the power of supported bilayers to address key questions in immunology and cell biology... - Molecular mechanisms in signal transduction at the membraneJay T Groves
Departments of Chemistry, University of California, Berkeley, California, USA
Nat Struct Mol Biol 17:659-65. 2010.... - Chemistry. Unveiling the membrane domainsJay T Groves
Department of Chemistry, University of California, Berkeley, CA 94720, USA
Science 313:1901-2. 2006 - Bending mechanics and molecular organization in biological membranesJay T Groves
Department of Chemistry, University of California, Berkeley, CA 94720, USA
Annu Rev Phys Chem 58:697-717. 2007..These results have emerged largely from new experimental methodologies, which are discussed in parallel... - Molecular organization and signal transduction at intermembrane junctionsJay T Groves
Department of Chemistry, University of California Berkeley, USA
Angew Chem Int Ed Engl 44:3524-38. 2005..This convergence is fueled be recent, exquisite observations of protein pattern formation events within living immunological synapses along with parallel advances in membrane reconstitution, manipulation, and imaging technologies... - Restriction of receptor movement alters cellular response: physical force sensing by EphA2Khalid Salaita
Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, CA 94720, USA
Science 327:1380-5. 2010..These observations reveal a mechanism for spatio-mechanical regulation of EphA2 signaling pathways... - TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activationBjörn F Lillemeier
Howard Hughes Medical Institute, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
Nat Immunol 11:90-6. 2010..This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction... - Effect of support corrugation on silica xerogel--supported phase-separated lipid bilayersEmel I Goksu
Department of Chemical Engineering and Materials Science, University of California, Davis, California 95616, USA
Langmuir 25:3713-7. 2009..Overall, our results suggest the presence of a relatively defect-free continuous two-phase lipid bilayer that penetrates approximately midway into the first layer of approximately 50 nm silica xerogel beads... - Interrogating the T cell synapse with patterned surfaces and photoactivated proteinsAndrew L DeMond
Biophysics Graduate Group, Berkeley, CA 94720, USA
Curr Opin Immunol 19:722-7. 2007..Recent results have demonstrated the effectiveness of these techniques as tools to investigate the mechanisms of immune synapse assembly and synaptic signaling... - Surface binding affinity measurements from order transitions of lipid membrane-coated colloidal particlesEsther M Winter
Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
Anal Chem 78:174-80. 2006..This is used to determine binding affinity based only on observations of the colloidal distribution... - Detection of molecular interactions at membrane surfaces through colloid phase transitionsMichael M Baksh
Department of Chemistry, University of California, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
Nature 427:139-41. 2004..We expect that this approach, used here to probe with high sensitivity protein binding events at membrane surfaces, can be applied to study a broad range of cell membrane processes... - T-cell triggering thresholds are modulated by the number of antigen within individual T-cell receptor clustersBoryana N Manz
Howard Hughes Medical Institute, Department of Chemistry, and Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 108:9089-94. 2011..This threshold is unchanged by inclusion of coagonist pMHC, but costimulation of CD28 by CD80 can modulate the threshold lower... - Noncovalent cell surface engineering: incorporation of bioactive synthetic glycopolymers into cellular membranesDavid Rabuka
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Am Chem Soc 130:5947-53. 2008..This system provides a platform to study cell-surface phenomena with a degree of chemical control that cannot be achieved using conventional biological tools... - Neuronal synapse interaction reconstituted between live cells and supported lipid bilayersSophie Pautot
Materials Sciences and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
Nat Chem Biol 1:283-9. 2005..The results suggest that Nlg-Nrx interaction rapidly establishes a weak, but specific, adhesion between dynamic pre- and postsynaptic processes, which may ultimately require additional molecules for synapse stabilization... - Lipid lateral mobility and membrane phase structure modulation by protein bindingMartin B Forstner
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Am Chem Soc 128:15221-7. 2006..02 area fraction. Spectral shifts of the methylene symmetric and asymmetric stretching modes in the lipid acyl chain confirm that protein binding alters the fraction of lipid in the gel phase... - Kinetic control of histidine-tagged protein surface density on supported lipid bilayersJeffrey A Nye
Department of Chemical Engineering and Department of Chemistry, University of California Berkeley, CA 94720, USA
Langmuir 24:4145-9. 2008..We propose guidelines for the assembly of SLB surfaces functionalized with specific protein densities and demonstrate their utility in the formation of hybrid immunological synapses... - Analysis of shape, fluctuations, and dynamics in intermembrane junctionsLawrence C-L Lin
Department of Physics, and Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, USA
Biophys J 91:3600-6. 2006..01-0.06 dyn/cm). The measurements suggest bilayer-bilayer adhesion energetics as the dominant source of surface tension in the experimental systems... - Phase segregation on different length scales in a model cell membrane systemJian Liu
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Phys Chem B 109:19960-9. 2005..Experiments that could further assess the importance of dipolar interactions for lateral organization of the components on multiple length scales in membranes are suggested... - Electrostatically driven spatial patterns in lipid membrane compositionRaghuveer Parthasarathy
Department of Chemistry, University of California, Berkeley, 94720, USA
Phys Rev Lett 95:048101. 2005..Surprisingly, the electrostatic potential of the proteins is a minor factor in the lipid reorganization; the protein size and its modulation of the junction topography play the dominant role in driving the electrostatic patterns... - Quantitative fluorescence microscopy using supported lipid bilayer standardsWilliam J Galush
Department of Chemistry, University of California, Berkeley, California, USA
Biophys J 95:2512-9. 2008..To illustrate the advantages of this approach, we quantify cell- and bilayer-side protein density patterns in a hybrid immunological synapse between a T-cell and a supported bilayer... - A nanocube plasmonic sensor for molecular binding on membrane surfacesWilliam J Galush
Department of Chemistry, University of California, Berkeley, California 94720, USA
Nano Lett 9:2077-82. 2009..In contrast to other plasmonic sensing techniques, this method features simple, solution-based device fabrication and readout. Static and dynamic protein/membrane binding are monitored and quantified... - Formation and spatio-temporal evolution of periodic structures in lipid bilayersSharon Rozovsky
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Am Chem Soc 127:36-7. 2005..Such long-range ordering principles may exist in more complicated membrane systems... - A fluid membrane-based soluble ligand-display system for live-cell assaysJwa-Min Nam
Department of Chemistry, University of California and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
Chembiochem 7:436-40. 2006 - Hydrodynamic damping of membrane thermal fluctuations near surfaces imaged by fluorescence interference microscopyYoshihisa Kaizuka
Department of Chemistry, University of California, Berkeley, California 94720, USA
Phys Rev Lett 96:118101. 2006..These observations provide experimental confirmation of recent theoretical models... - Scanning probe lithography on fluid lipid membranesBryan L Jackson
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Am Chem Soc 126:13878-9. 2004..In the present study, bilayers of unique composition fill 1 x 1 mum corrals and were positioned 100 nm apart... - Coupled membrane fluctuations and protein mobility in supported intermembrane junctionsRaghuveer Parthasarathy
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Phys Chem B 110:8513-6. 2006.... - Micropatterned supported membranes as tools for quantitative studies of the immunological synapseKaspar Mossman
Biophysics Graduate Group, University of California, Berkeley, California, USA
Chem Soc Rev 36:46-54. 2007..In this tutorial review, we introduce the IS; discuss SLB techniques, including micropatterning; and discuss various methods used to perturb and quantify the IS... - Synthetic analogues of glycosylphosphatidylinositol-anchored proteins and their behavior in supported lipid bilayersMargot G Paulick
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Am Chem Soc 129:11543-50. 2007..The ability to generate chemically defined analogues of GPI-anchored proteins is an important step toward elucidating the molecular functions of this interesting post-translational modification... - Molecular orientation of membrane-anchored mucin glycoprotein mimicsRaghuveer Parthasarathy
Department of Chemistry, University of California, Berkeley, California 94720, USA
J Phys Chem B 111:12133-5. 2007..Rigidity and core glycosylation are therefore insufficient to ensure molecular projection outward from a membrane surface... - T cell receptor microcluster transport through molecular mazes reveals mechanism of translocationAndrew L DeMond
Biophysics Graduate Group, University of California, Berkeley, California 94720, USA
Biophys J 94:3286-92. 2008..These data are consistent with a frictional model of TCR coupling to cytoskeletal flow, which allows slip. Implications of this model regarding spatial sorting of cell-surface molecules are discussed... - Membrane-dependent signal integration by the Ras activator Son of sevenlessJodi Gureasko
Department of Molecular and Cell Biology, and Howard Hughes Medical Institute, QB3 Institute, 176 Stanley Hall, University of California, Berkeley, California 94720, USA
Nat Struct Mol Biol 15:452-61. 2008.... - Engineering of a synthetic electron conduit in living cellsHeather M Jensen
Department of Chemistry and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 107:19213-8. 2010.... - Cluster size regulates protein sorting in the immunological synapseNiña C Hartman
Department of Chemistry, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 106:12729-34. 2009..We discuss a sorting mechanism, based on frictional coupling to the actin cytoskeleton, that is consistent with these observations and is, in principle, extendable to all cell surface proteins in the synapse... - Membrane array technology for drug discoveryJay T Groves
University of California, Department of Chemistry, Berkeley, CA 94720, USA
Curr Opin Drug Discov Devel 5:606-12. 2002..Recent developments in membrane array manufacturing are positioning this technology to make the transition into industrial drug discovery programs... - Micropattern formation in supported lipid membranesJay T Groves
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Acc Chem Res 35:149-57. 2002..Collectively, these preparative and analytical patterned membrane techniques offer a broad experimental platform for the study and utilization of lipid membranes... - Neuronal activation by GPI-linked neuroligin-1 displayed in synthetic lipid bilayer membranesMichael M Baksh
Department of Chemistry, University of California Berkeley, 94720, USA
Langmuir 21:10693-8. 2005..In addition, the synthetic membrane environment affords control over both lipid and protein composition. This technology is easily implemented and can be applied to a wide variety of cellular studies... - Altered TCR signaling from geometrically repatterned immunological synapsesKaspar D Mossman
Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA
Science 310:1191-3. 2005..These results are consistent with a model of the synapse in which spatial translocation of TCRs represents a direct mechanism of signal regulation... - Curvature-modulated phase separation in lipid bilayer membranesRaghuveer Parthasarathy
Department of Chemistry, University of California, Berkeley, California 94720, USA
Langmuir 22:5095-9. 2006..Curvature-mediated patterning may also be useful in controlling complex fluids other than biomembranes... - Nonequilibrium patterns of cholesterol-rich chemical heterogenieties within single fluid supported phospholipid bilayer membranesAnnapoorna R Sapuri-Butti
Department of Applied Science, University of California, Davis, California 95616, USA
Langmuir 22:5374-84. 2006.... - Structure and dynamics of supported intermembrane junctionsYoshihisa Kaizuka
Department of Chemistry, University of California, Berkeley, California 94720, USA
Biophys J 86:905-12. 2004..The results provide experimental verification of a theoretical model for two-dimensional mobility in membranes, which includes frictional coupling across an interstitial water layer... - Control of antigen presentation with a photoreleasable agonist peptideAndrew L DeMond
Biophysics Graduate Group and Department of Chemistry, University of California, Berkeley, California 94720, USA
J Am Chem Soc 128:15354-5. 2006..Irradiation with UV light was sufficient to restore agonist activity in situ... - Hybrid protein-lipid patterns from aluminum templatesBryan L Jackson
Department of Chemistry, University of California, Berkeley, California 94720, USA
Langmuir 23:2052-7. 2007..Inorganic surfaces were characterized by atomic force microscopy and X-ray photoelectron spectroscopy while supported bilayers and protein patterns were characterized by epifluorescence microscopy... - The synapse assembly modelSung-Joo E Lee
Biophysics Graduate Group, Dept of Chemistry, University of California, Berkeley, CA, USA
Trends Immunol 23:500-2. 2002..This model uses partial differential equations to describe the binding interactions of receptors and ligands, with the constraint that they are embedded in apposed deformable membranes linked to a cytoskeletal complex... - Correlation of a dynamic model for immunological synapse formation with effector functions: two pathways to synapse formationSung-Joo E Lee
Biophysics Graduate Group, Dept of Chemistry, University of California, Berkeley, CA, USA
Trends Immunol 23:492-9. 2002.... - Protein patterns at lipid bilayer junctionsRaghuveer Parthasarathy
Department of Chemistry, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 101:12798-803. 2004..These studies demonstrate that membrane mechanical forces alone, i.e., in the absence of specific biochemical interactions, can drive microm-scale organization of membrane proteins... - Molecular topography imaging by intermembrane fluorescence resonance energy transferAmy P Wong
Department of Chemistry, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 99:14147-52. 2002..In the case of polyethylene glycol lipid-containing membrane junctions, imaging by intermembrane FRET reveals spontaneously forming patterns that are not visible in conventional fluorescence images... - Spatial organization and signal transduction at intercellular junctionsBoryana N Manz
Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, CA 94720, USA
Nat Rev Mol Cell Biol 11:342-52. 2010..The resulting spatial mutations yield insights into the interweaving of the spatial, mechanical and chemical aspects of intercellular signalling... - Kinetic pathways of phase ordering in lipid raft model systemsJian Liu
Department of Chemistry, Department of Chemical Engineering, Biophysics Graduate Group, University of California, Berkeley, California 94720, USA
J Phys Chem B 110:8416-21. 2006..Our results suggest experiments with synthetic vesicles where the stripelike patterns could be observed... - Synthesis of lipidated green fluorescent protein and its incorporation in supported lipid bilayersMichael J Grogan
Departments of Chemistry and Molecular and Cell Biology, Physical Biosciences and Materials Sciences Divisions, University of California-Berkeley, Berkeley, CA 94720, USA
J Am Chem Soc 127:14383-7. 2005..Circumventing the steps of purification from native cell membranes, this methodology facilitates the reconstitution of membrane-associated proteins... - Discrete arrays of liquid-crystal-supported proteolipid monolayers as phantom cell surfacesAmber R Wise
University of California Berkeley, Chemistry Department, Berkeley, CA 94720, USA
Chemphyschem 9:1688-92. 2008..Additionally, experiments with live immunological T cells forming active synapses at the interface exhibit the specific nature of the surface... - A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchorMargot G Paulick
Departments of Chemistry and Molecular and Cell Biology, and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 104:20332-7. 2007..Thus, this cellular system provides a platform for dissecting the contributions of various GPI anchor components to their biological function... - Hierarchical assembly of model cell surfaces: synthesis of mucin mimetic polymers and their display on supported bilayersDavid Rabuka
Department of Chemistry, Howard Hughes Medical Institute, University of California, California, USA
J Am Chem Soc 129:5462-71. 2007..This system provides a unique framework with which to study the behavior of mucin-like macromolecules in a controlled, cell surface-mimetic environment... - Lipid mobility and molecular binding in fluid lipid membranesVictoria Yamazaki
Synamem Corporation, Burlingame, California 94010, USA
J Am Chem Soc 127:2826-7. 2005..Experiments using membrane microarrays are performed that illustrate the use of lipid mobility measurements as an effectively label-free strategy of detecting binding on membrane surfaces... - Detection of proteins using a colorimetric bio-barcode assayJwa Min Nam
Department of Chemistry, Seoul National University, San 56 1, Sillim dong, Gwanak Gu, Seoul 151 747, South Korea
Nat Protoc 2:1438-44. 2007..Actual target detection and quantification steps with premade probes take approximately 3 h (whole protocol including probe preparations takes approximately 3 days)...
Research Grants
- Quantitative Studies of the Immunological SynapseJay Groves; Fiscal Year: 2005..These studies will provide a deep and quantitative understanding of synaptic pattern formation in the immune system and may lead to novel biomimetic and therapeutic approaches. ..