Jay Groves

Summary

Affiliation: Lawrence Berkeley National Laboratory
Country: USA

Publications

  1. ncbi request reprint Electrical manipulation of supported lipid membranes by embedded electrodes
    Bryan L Jackson
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Langmuir 24:6189-93. 2008
  2. pmc A nanocube plasmonic sensor for molecular binding on membrane surfaces
    William J Galush
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Nano Lett 9:2077-82. 2009
  3. pmc Supported membranes embedded with fixed arrays of gold nanoparticles
    Theobald Lohmüller
    Howard Hughes Medical Institute and Department of Chemistry, University of California, Berkeley, Berkeley, California 94720, United States
    Nano Lett 11:4912-8. 2011
  4. pmc Patterned two-photon photoactivation illuminates spatial reorganization in live cells
    Adam W Smith
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California 94720, United States
    J Phys Chem A 115:3867-75. 2011
  5. pmc Myosin IIA modulates T cell receptor transport and CasL phosphorylation during early immunological synapse formation
    Yan Yu
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California, United States of America
    PLoS ONE 7:e30704. 2012
  6. pmc Altered actin centripetal retrograde flow in physically restricted immunological synapses
    Cheng Han Yu
    Research Centre of Excellence in Mechanobiology, National University of Singapore, Singapore, Singapore
    PLoS ONE 5:e11878. 2010
  7. pmc Single molecule tracking on supported membranes with arrays of optical nanoantennas
    T Lohmüller
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California 94720, USA
    Nano Lett 12:1717-21. 2012
  8. pmc Monitoring lipid anchor organization in cell membranes by PIE-FCCS
    Sara B Triffo
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 134:10833-42. 2012
  9. doi request reprint DNA-mediated assembly of protein heterodimers on membrane surfaces
    Michael P Coyle
    Department of Chemistry and Howard Hughes Medical Institute, University of California, Berkeley, California 94720, United States
    J Am Chem Soc 135:5012-6. 2013
  10. pmc Engineering supported membranes for cell biology
    Cheng Han Yu
    Research Centre of Excellence in Mechanobiology, National University of Singapore, Singapore
    Med Biol Eng Comput 48:955-63. 2010

Collaborators

Detail Information

Publications69

  1. ncbi request reprint Electrical manipulation of supported lipid membranes by embedded electrodes
    Bryan L Jackson
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Langmuir 24:6189-93. 2008
    ..Application of lower magnitude direct current fields resulted in electrophoretic redistribution of the membrane probes and electro-osmotic reorganization of membrane associated proteins...
  2. pmc A nanocube plasmonic sensor for molecular binding on membrane surfaces
    William J Galush
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Nano Lett 9:2077-82. 2009
    ..In contrast to other plasmonic sensing techniques, this method features simple, solution-based device fabrication and readout. Static and dynamic protein/membrane binding are monitored and quantified...
  3. pmc Supported membranes embedded with fixed arrays of gold nanoparticles
    Theobald Lohmüller
    Howard Hughes Medical Institute and Department of Chemistry, University of California, Berkeley, Berkeley, California 94720, United States
    Nano Lett 11:4912-8. 2011
    ..Finally, the functionality of this system for live cell experiments is tested using the ephrin-A1-EphA2 juxtacrine signaling interaction in human breast epithelial cells...
  4. pmc Patterned two-photon photoactivation illuminates spatial reorganization in live cells
    Adam W Smith
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California 94720, United States
    J Phys Chem A 115:3867-75. 2011
    ..The combination of patterned photoactivation and SVD analysis offers a unique way to measure spatial redistribution dynamics within live cells...
  5. pmc Myosin IIA modulates T cell receptor transport and CasL phosphorylation during early immunological synapse formation
    Yan Yu
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California, United States of America
    PLoS ONE 7:e30704. 2012
    ....
  6. pmc Altered actin centripetal retrograde flow in physically restricted immunological synapses
    Cheng Han Yu
    Research Centre of Excellence in Mechanobiology, National University of Singapore, Singapore, Singapore
    PLoS ONE 5:e11878. 2010
    ..Actin flow regains its velocity immediately after passing through confined TCR clusters. These observations are consistent with a dynamic and dissipative coupling between TCR clusters and viscoelastic actin network...
  7. pmc Single molecule tracking on supported membranes with arrays of optical nanoantennas
    T Lohmüller
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California 94720, USA
    Nano Lett 12:1717-21. 2012
    ..The nanoantenna array thus enables enhanced observation of a fluid membrane system without static entrapment of the molecules...
  8. pmc Monitoring lipid anchor organization in cell membranes by PIE-FCCS
    Sara B Triffo
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 134:10833-42. 2012
    ..Importantly, these observations do not support a simple interpretation of lipid anchor-mediated organization driven by partitioning based on binary lipid phase separation...
  9. doi request reprint DNA-mediated assembly of protein heterodimers on membrane surfaces
    Michael P Coyle
    Department of Chemistry and Howard Hughes Medical Institute, University of California, Berkeley, California 94720, United States
    J Am Chem Soc 135:5012-6. 2013
    ..We further demonstrate that proteins linked in this manner as either monomers or dimers can form functional interfaces with living cells. ..
  10. pmc Engineering supported membranes for cell biology
    Cheng Han Yu
    Research Centre of Excellence in Mechanobiology, National University of Singapore, Singapore
    Med Biol Eng Comput 48:955-63. 2010
    ..The integration of biological components into synthetic devices provides a unique approach to investigate molecular mechanisms in cell biology...
  11. pmc Cell membrane array fabrication and assay technology
    Victoria Yamazaki
    Synamem Corporation, 863 Mitten Road, Suite 101, Burlingame, CA 94010, USA
    BMC Biotechnol 5:18. 2005
    ..By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processes has been considerably more difficult...
  12. ncbi request reprint Bending mechanics and molecular organization in biological membranes
    Jay T Groves
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Annu Rev Phys Chem 58:697-717. 2007
    ..These results have emerged largely from new experimental methodologies, which are discussed in parallel...
  13. pmc Molecular mechanisms in signal transduction at the membrane
    Jay T Groves
    Departments of Chemistry, University of California, Berkeley, California, USA
    Nat Struct Mol Biol 17:659-65. 2010
    ....
  14. ncbi request reprint Chemistry. Unveiling the membrane domains
    Jay T Groves
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 313:1901-2. 2006
  15. ncbi request reprint Spatial mutation of the T cell immunological synapse
    Jay T Groves
    Department of Chemistry, University of California Berkeley, Berkeley, CA 94720, USA
    Curr Opin Chem Biol 10:544-50. 2006
    ..These methodologies complement conventional genetic and pharmacological approaches, both of which are chemical in nature, by perturbing the system through exclusively physical mechanisms...
  16. doi request reprint Fluorescence imaging of membrane dynamics
    Jay T Groves
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Annu Rev Biomed Eng 10:311-38. 2008
    ..For each, we discuss the physics underpinning the approach, its practical implementation, and recent examples highlighting its achievements in exploring the membrane environment...
  17. ncbi request reprint Supported planar bilayers in studies on immune cell adhesion and communication
    Jay T Groves
    Department of Chemistry and Physical Biosciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    J Immunol Methods 278:19-32. 2003
    ..Supported planar bilayers are simple to form by liposome fusion and recent advances in micro- and nanotechnology greatly extend the power of supported bilayers to address key questions in immunology and cell biology...
  18. ncbi request reprint Learning the chemical language of cell-surface interactions
    Jay T Groves
    Department of Chemistry, University of California, Berkeley, USA
    Sci STKE 2005:pe45. 2005
    ..A number of promising advances in the development of synthetic interfaces with cells suggest that the move to predictive design is under way...
  19. ncbi request reprint Molecular organization and signal transduction at intermembrane junctions
    Jay T Groves
    Department of Chemistry, University of California Berkeley, USA
    Angew Chem Int Ed Engl 44:3524-38. 2005
    ..This convergence is fueled be recent, exquisite observations of protein pattern formation events within living immunological synapses along with parallel advances in membrane reconstitution, manipulation, and imaging technologies...
  20. pmc Restriction of receptor movement alters cellular response: physical force sensing by EphA2
    Khalid Salaita
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 327:1380-5. 2010
    ..These observations reveal a mechanism for spatio-mechanical regulation of EphA2 signaling pathways...
  21. pmc TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation
    Björn F Lillemeier
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
    Nat Immunol 11:90-6. 2010
    ..This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction...
  22. ncbi request reprint Interrogating the T cell synapse with patterned surfaces and photoactivated proteins
    Andrew L DeMond
    Biophysics Graduate Group, Berkeley, CA 94720, USA
    Curr Opin Immunol 19:722-7. 2007
    ..Recent results have demonstrated the effectiveness of these techniques as tools to investigate the mechanisms of immune synapse assembly and synaptic signaling...
  23. ncbi request reprint Effect of support corrugation on silica xerogel--supported phase-separated lipid bilayers
    Emel I Goksu
    Department of Chemical Engineering and Materials Science, University of California, Davis, California 95616, USA
    Langmuir 25:3713-7. 2009
    ..Overall, our results suggest the presence of a relatively defect-free continuous two-phase lipid bilayer that penetrates approximately midway into the first layer of approximately 50 nm silica xerogel beads...
  24. ncbi request reprint Surface binding affinity measurements from order transitions of lipid membrane-coated colloidal particles
    Esther M Winter
    Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
    Anal Chem 78:174-80. 2006
    ..This is used to determine binding affinity based only on observations of the colloidal distribution...
  25. ncbi request reprint Detection of molecular interactions at membrane surfaces through colloid phase transitions
    Michael M Baksh
    Department of Chemistry, University of California, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nature 427:139-41. 2004
    ..We expect that this approach, used here to probe with high sensitivity protein binding events at membrane surfaces, can be applied to study a broad range of cell membrane processes...
  26. pmc T-cell triggering thresholds are modulated by the number of antigen within individual T-cell receptor clusters
    Boryana N Manz
    Howard Hughes Medical Institute, Department of Chemistry, and Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:9089-94. 2011
    ..This threshold is unchanged by inclusion of coagonist pMHC, but costimulation of CD28 by CD80 can modulate the threshold lower...
  27. pmc Noncovalent cell surface engineering: incorporation of bioactive synthetic glycopolymers into cellular membranes
    David Rabuka
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 130:5947-53. 2008
    ..This system provides a platform to study cell-surface phenomena with a degree of chemical control that cannot be achieved using conventional biological tools...
  28. doi request reprint Kinetic control of histidine-tagged protein surface density on supported lipid bilayers
    Jeffrey A Nye
    Department of Chemical Engineering and Department of Chemistry, University of California Berkeley, CA 94720, USA
    Langmuir 24:4145-9. 2008
    ..We propose guidelines for the assembly of SLB surfaces functionalized with specific protein densities and demonstrate their utility in the formation of hybrid immunological synapses...
  29. pmc Neuronal synapse interaction reconstituted between live cells and supported lipid bilayers
    Sophie Pautot
    Materials Sciences and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nat Chem Biol 1:283-9. 2005
    ..The results suggest that Nlg-Nrx interaction rapidly establishes a weak, but specific, adhesion between dynamic pre- and postsynaptic processes, which may ultimately require additional molecules for synapse stabilization...
  30. ncbi request reprint Lipid lateral mobility and membrane phase structure modulation by protein binding
    Martin B Forstner
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 128:15221-7. 2006
    ..02 area fraction. Spectral shifts of the methylene symmetric and asymmetric stretching modes in the lipid acyl chain confirm that protein binding alters the fraction of lipid in the gel phase...
  31. ncbi request reprint Scanning probe lithography on fluid lipid membranes
    Bryan L Jackson
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 126:13878-9. 2004
    ..In the present study, bilayers of unique composition fill 1 x 1 mum corrals and were positioned 100 nm apart...
  32. ncbi request reprint Coupled membrane fluctuations and protein mobility in supported intermembrane junctions
    Raghuveer Parthasarathy
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Phys Chem B 110:8513-6. 2006
    ....
  33. pmc Analysis of shape, fluctuations, and dynamics in intermembrane junctions
    Lawrence C L Lin
    Department of Physics, and Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, USA
    Biophys J 91:3600-6. 2006
    ..01-0.06 dyn/cm). The measurements suggest bilayer-bilayer adhesion energetics as the dominant source of surface tension in the experimental systems...
  34. ncbi request reprint Formation and spatio-temporal evolution of periodic structures in lipid bilayers
    Sharon Rozovsky
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 127:36-7. 2005
    ..Such long-range ordering principles may exist in more complicated membrane systems...
  35. ncbi request reprint Electrostatically driven spatial patterns in lipid membrane composition
    Raghuveer Parthasarathy
    Department of Chemistry, University of California, Berkeley, 94720, USA
    Phys Rev Lett 95:048101. 2005
    ..Surprisingly, the electrostatic potential of the proteins is a minor factor in the lipid reorganization; the protein size and its modulation of the junction topography play the dominant role in driving the electrostatic patterns...
  36. ncbi request reprint Hydrodynamic damping of membrane thermal fluctuations near surfaces imaged by fluorescence interference microscopy
    Yoshihisa Kaizuka
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Phys Rev Lett 96:118101. 2006
    ..These observations provide experimental confirmation of recent theoretical models...
  37. pmc A fluid membrane-based soluble ligand-display system for live-cell assays
    Jwa Min Nam
    Department of Chemistry, University of California and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Chembiochem 7:436-40. 2006
  38. ncbi request reprint Phase segregation on different length scales in a model cell membrane system
    Jian Liu
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Phys Chem B 109:19960-9. 2005
    ..Experiments that could further assess the importance of dipolar interactions for lateral organization of the components on multiple length scales in membranes are suggested...
  39. pmc Quantitative fluorescence microscopy using supported lipid bilayer standards
    William J Galush
    Department of Chemistry, University of California, Berkeley, California, USA
    Biophys J 95:2512-9. 2008
    ..To illustrate the advantages of this approach, we quantify cell- and bilayer-side protein density patterns in a hybrid immunological synapse between a T-cell and a supported bilayer...
  40. ncbi request reprint Micropatterned supported membranes as tools for quantitative studies of the immunological synapse
    Kaspar Mossman
    Biophysics Graduate Group, University of California, Berkeley, California, USA
    Chem Soc Rev 36:46-54. 2007
    ..In this tutorial review, we introduce the IS; discuss SLB techniques, including micropatterning; and discuss various methods used to perturb and quantify the IS...
  41. pmc Engineering of a synthetic electron conduit in living cells
    Heather M Jensen
    Department of Chemistry and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 107:19213-8. 2010
    ....
  42. pmc Protein patterns at lipid bilayer junctions
    Raghuveer Parthasarathy
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 101:12798-803. 2004
    ..These studies demonstrate that membrane mechanical forces alone, i.e., in the absence of specific biochemical interactions, can drive microm-scale organization of membrane proteins...
  43. pmc Membrane-dependent signal integration by the Ras activator Son of sevenless
    Jodi Gureasko
    Department of Molecular and Cell Biology, and Howard Hughes Medical Institute, QB3 Institute, 176 Stanley Hall, University of California, Berkeley, California 94720, USA
    Nat Struct Mol Biol 15:452-61. 2008
    ....
  44. pmc Cluster size regulates protein sorting in the immunological synapse
    Niña C Hartman
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 106:12729-34. 2009
    ..We discuss a sorting mechanism, based on frictional coupling to the actin cytoskeleton, that is consistent with these observations and is, in principle, extendable to all cell surface proteins in the synapse...
  45. pmc T cell receptor microcluster transport through molecular mazes reveals mechanism of translocation
    Andrew L DeMond
    Biophysics Graduate Group, University of California, Berkeley, California 94720, USA
    Biophys J 94:3286-92. 2008
    ..These data are consistent with a frictional model of TCR coupling to cytoskeletal flow, which allows slip. Implications of this model regarding spatial sorting of cell-surface molecules are discussed...
  46. ncbi request reprint Membrane array technology for drug discovery
    Jay T Groves
    University of California, Department of Chemistry, Berkeley, CA 94720, USA
    Curr Opin Drug Discov Devel 5:606-12. 2002
    ..Recent developments in membrane array manufacturing are positioning this technology to make the transition into industrial drug discovery programs...
  47. ncbi request reprint Correlation of a dynamic model for immunological synapse formation with effector functions: two pathways to synapse formation
    Sung Joo E Lee
    Biophysics Graduate Group, Dept of Chemistry, University of California, Berkeley, CA, USA
    Trends Immunol 23:492-9. 2002
    ....
  48. ncbi request reprint The synapse assembly model
    Sung Joo E Lee
    Biophysics Graduate Group, Dept of Chemistry, University of California, Berkeley, CA, USA
    Trends Immunol 23:500-2. 2002
    ..This model uses partial differential equations to describe the binding interactions of receptors and ligands, with the constraint that they are embedded in apposed deformable membranes linked to a cytoskeletal complex...
  49. pmc Structure and dynamics of supported intermembrane junctions
    Yoshihisa Kaizuka
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Biophys J 86:905-12. 2004
    ..The results provide experimental verification of a theoretical model for two-dimensional mobility in membranes, which includes frictional coupling across an interstitial water layer...
  50. pmc Neuronal activation by GPI-linked neuroligin-1 displayed in synthetic lipid bilayer membranes
    Michael M Baksh
    Department of Chemistry, University of California Berkeley, 94720, USA
    Langmuir 21:10693-8. 2005
    ..In addition, the synthetic membrane environment affords control over both lipid and protein composition. This technology is easily implemented and can be applied to a wide variety of cellular studies...
  51. ncbi request reprint Altered TCR signaling from geometrically repatterned immunological synapses
    Kaspar D Mossman
    Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA
    Science 310:1191-3. 2005
    ..These results are consistent with a model of the synapse in which spatial translocation of TCRs represents a direct mechanism of signal regulation...
  52. ncbi request reprint Curvature-modulated phase separation in lipid bilayer membranes
    Raghuveer Parthasarathy
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Langmuir 22:5095-9. 2006
    ..Curvature-mediated patterning may also be useful in controlling complex fluids other than biomembranes...
  53. ncbi request reprint Nonequilibrium patterns of cholesterol-rich chemical heterogenieties within single fluid supported phospholipid bilayer membranes
    Annapoorna R Sapuri-Butti
    Department of Applied Science, University of California, Davis, California 95616, USA
    Langmuir 22:5374-84. 2006
    ....
  54. ncbi request reprint Micropattern formation in supported lipid membranes
    Jay T Groves
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Acc Chem Res 35:149-57. 2002
    ..Collectively, these preparative and analytical patterned membrane techniques offer a broad experimental platform for the study and utilization of lipid membranes...
  55. ncbi request reprint Control of antigen presentation with a photoreleasable agonist peptide
    Andrew L DeMond
    Biophysics Graduate Group and Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 128:15354-5. 2006
    ..Irradiation with UV light was sufficient to restore agonist activity in situ...
  56. ncbi request reprint Hybrid protein-lipid patterns from aluminum templates
    Bryan L Jackson
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Langmuir 23:2052-7. 2007
    ..Inorganic surfaces were characterized by atomic force microscopy and X-ray photoelectron spectroscopy while supported bilayers and protein patterns were characterized by epifluorescence microscopy...
  57. ncbi request reprint Molecular orientation of membrane-anchored mucin glycoprotein mimics
    Raghuveer Parthasarathy
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Phys Chem B 111:12133-5. 2007
    ..Rigidity and core glycosylation are therefore insufficient to ensure molecular projection outward from a membrane surface...
  58. ncbi request reprint Synthetic analogues of glycosylphosphatidylinositol-anchored proteins and their behavior in supported lipid bilayers
    Margot G Paulick
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    J Am Chem Soc 129:11543-50. 2007
    ..The ability to generate chemically defined analogues of GPI-anchored proteins is an important step toward elucidating the molecular functions of this interesting post-translational modification...
  59. pmc A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor
    Margot G Paulick
    Departments of Chemistry and Molecular and Cell Biology, and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:20332-7. 2007
    ..Thus, this cellular system provides a platform for dissecting the contributions of various GPI anchor components to their biological function...
  60. ncbi request reprint Discrete arrays of liquid-crystal-supported proteolipid monolayers as phantom cell surfaces
    Amber R Wise
    University of California Berkeley, Chemistry Department, Berkeley, CA 94720, USA
    Chemphyschem 9:1688-92. 2008
    ..Additionally, experiments with live immunological T cells forming active synapses at the interface exhibit the specific nature of the surface...
  61. pmc Molecular topography imaging by intermembrane fluorescence resonance energy transfer
    Amy P Wong
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 99:14147-52. 2002
    ..In the case of polyethylene glycol lipid-containing membrane junctions, imaging by intermembrane FRET reveals spontaneously forming patterns that are not visible in conventional fluorescence images...
  62. pmc Spatial organization and signal transduction at intercellular junctions
    Boryana N Manz
    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Nat Rev Mol Cell Biol 11:342-52. 2010
    ..The resulting spatial mutations yield insights into the interweaving of the spatial, mechanical and chemical aspects of intercellular signalling...
  63. ncbi request reprint Synthesis of lipidated green fluorescent protein and its incorporation in supported lipid bilayers
    Michael J Grogan
    Departments of Chemistry and Molecular and Cell Biology, Physical Biosciences and Materials Sciences Divisions, University of California Berkeley, Berkeley, CA 94720, USA
    J Am Chem Soc 127:14383-7. 2005
    ..Circumventing the steps of purification from native cell membranes, this methodology facilitates the reconstitution of membrane-associated proteins...
  64. pmc Hierarchical assembly of model cell surfaces: synthesis of mucin mimetic polymers and their display on supported bilayers
    David Rabuka
    Department of Chemistry, Howard Hughes Medical Institute, University of California, California, USA
    J Am Chem Soc 129:5462-71. 2007
    ..This system provides a unique framework with which to study the behavior of mucin-like macromolecules in a controlled, cell surface-mimetic environment...
  65. ncbi request reprint Kinetic pathways of phase ordering in lipid raft model systems
    Jian Liu
    Department of Chemistry, Department of Chemical Engineering, Biophysics Graduate Group, University of California, Berkeley, California 94720, USA
    J Phys Chem B 110:8416-21. 2006
    ..Our results suggest experiments with synthetic vesicles where the stripelike patterns could be observed...
  66. ncbi request reprint Lipid mobility and molecular binding in fluid lipid membranes
    Victoria Yamazaki
    Synamem Corporation, Burlingame, California 94010, USA
    J Am Chem Soc 127:2826-7. 2005
    ..Experiments using membrane microarrays are performed that illustrate the use of lipid mobility measurements as an effectively label-free strategy of detecting binding on membrane surfaces...
  67. ncbi request reprint Detection of proteins using a colorimetric bio-barcode assay
    Jwa Min Nam
    Department of Chemistry, Seoul National University, San 56 1, Sillim dong, Gwanak Gu, Seoul 151 747, South Korea
    Nat Protoc 2:1438-44. 2007
    ..Actual target detection and quantification steps with premade probes take approximately 3 h (whole protocol including probe preparations takes approximately 3 days)...

Research Grants5

  1. Quantitative Studies of the Immunological Synapse
    Jay Groves; Fiscal Year: 2002
    ..These studies will provide a deep and quantitative understanding of synaptic pattern formation in the immune system and may lead to novel biomimetic and therapeutic approaches. ..
  2. Quantitative Studies of the Immunological Synapse
    Jay Groves; Fiscal Year: 2003
    ..These studies will provide a deep and quantitative understanding of synaptic pattern formation in the immune system and may lead to novel biomimetic and therapeutic approaches. ..
  3. Quantitative Studies of the Immunological Synapse
    Jay Groves; Fiscal Year: 2004
    ..These studies will provide a deep and quantitative understanding of synaptic pattern formation in the immune system and may lead to novel biomimetic and therapeutic approaches. ..
  4. Quantitative Studies of the Immunological Synapse
    Jay Groves; Fiscal Year: 2005
    ..These studies will provide a deep and quantitative understanding of synaptic pattern formation in the immune system and may lead to novel biomimetic and therapeutic approaches. ..
  5. Quantitative Studies of the Immunological Synapse
    Jay Groves; Fiscal Year: 2005
    ..These studies will provide a deep and quantitative understanding of synaptic pattern formation in the immune system and may lead to novel biomimetic and therapeutic approaches. ..