Klaus Ley

Summary

Affiliation: La Jolla Institute for Allergy and Immunology
Country: USA

Publications

  1. pmc Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling
    Prithu Sundd
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA
    Nat Methods 7:821-4. 2010
  2. pmc PSGL-1 engagement by E-selectin signals through Src kinase Fgr and ITAM adapters DAP12 and FcR gamma to induce slow leukocyte rolling
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Exp Med 205:2339-47. 2008
  3. pmc 'Slings' enable neutrophil rolling at high shear
    Prithu Sundd
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA
    Nature 488:399-403. 2012
  4. pmc Neutrophil arrest by LFA-1 activation
    Craig T Lefort
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA
    Front Immunol 3:157. 2012
  5. ncbi request reprint Getting to the site of inflammation: the leukocyte adhesion cascade updated
    Klaus Ley
    Robert M Berne Cardiovascular Research Center and Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Rev Immunol 7:678-89. 2007
  6. pmc Dances with leukocytes: how tetraspanin-enriched microdomains assemble to form endothelial adhesive platforms
    Klaus Ley
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Cell Biol 183:375-6. 2008
  7. pmc Monocyte and macrophage dynamics during atherogenesis
    Klaus Ley
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 31:1506-16. 2011
  8. pmc Preferential migration of effector CD8+ T cells into the interstitium of the normal lung
    Elena Galkina
    Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    J Clin Invest 115:3473-83. 2005
  9. pmc Spleen tyrosine kinase Syk is necessary for E-selectin-induced alpha(L)beta(2) integrin-mediated rolling on intercellular adhesion molecule-1
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Immunity 26:773-83. 2007
  10. ncbi request reprint Mac-1, but not LFA-1, uses intercellular adhesion molecule-1 to mediate slow leukocyte rolling in TNF-alpha-induced inflammation
    Jessica L Dunne
    Department of Biomedical Engineering and Cardiovascular Research Center, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Immunol 171:6105-11. 2003

Collaborators

Detail Information

Publications102 found, 100 shown here

  1. pmc Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling
    Prithu Sundd
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA
    Nat Methods 7:821-4. 2010
    ..Each rolling neutrophil formed three to four long tethers that extended up to 16 μm behind the rolling cell...
  2. pmc PSGL-1 engagement by E-selectin signals through Src kinase Fgr and ITAM adapters DAP12 and FcR gamma to induce slow leukocyte rolling
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Exp Med 205:2339-47. 2008
    ....
  3. pmc 'Slings' enable neutrophil rolling at high shear
    Prithu Sundd
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA
    Nature 488:399-403. 2012
    ..Each sling effectively lays out a cell-autonomous adhesive substrate in front of neutrophils rolling at high shear stress during inflammation...
  4. pmc Neutrophil arrest by LFA-1 activation
    Craig T Lefort
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA
    Front Immunol 3:157. 2012
    ..This review integrates these new results into existing models of LFA-1 activation...
  5. ncbi request reprint Getting to the site of inflammation: the leukocyte adhesion cascade updated
    Klaus Ley
    Robert M Berne Cardiovascular Research Center and Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Rev Immunol 7:678-89. 2007
    ..This Review focuses on new aspects of one of the central paradigms of inflammation and immunity--the leukocyte adhesion cascade...
  6. pmc Dances with leukocytes: how tetraspanin-enriched microdomains assemble to form endothelial adhesive platforms
    Klaus Ley
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Cell Biol 183:375-6. 2008
    ..Furthermore, convincing biochemical data suggest that these structures are distinct from lipid rafts...
  7. pmc Monocyte and macrophage dynamics during atherogenesis
    Klaus Ley
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 31:1506-16. 2011
    ..In this review, we discuss these and other processes associated with monocytic cell dynamics in the vascular wall and their role in the initiation and progression of atherosclerosis...
  8. pmc Preferential migration of effector CD8+ T cells into the interstitium of the normal lung
    Elena Galkina
    Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    J Clin Invest 115:3473-83. 2005
    ..These results document a novel mechanism of adhesion receptor- and pulmonary chemokine-dependent regulation of the migration of activated CD8+ T cells into an important nonlymphoid peripheral site (i.e., the normal/noninflamed lung)...
  9. pmc Spleen tyrosine kinase Syk is necessary for E-selectin-induced alpha(L)beta(2) integrin-mediated rolling on intercellular adhesion molecule-1
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Immunity 26:773-83. 2007
    ..This identifies a second neutrophil-activation pathway that is as important as activation through G protein-coupled receptors (GPCRs)...
  10. ncbi request reprint Mac-1, but not LFA-1, uses intercellular adhesion molecule-1 to mediate slow leukocyte rolling in TNF-alpha-induced inflammation
    Jessica L Dunne
    Department of Biomedical Engineering and Cardiovascular Research Center, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Immunol 171:6105-11. 2003
    ..However, blocking both Mac-1 and ICAM-1 had no greater effect than either blockade alone. We conclude that endothelial ICAM-1 is the main ligand responsible for slow leukocyte rolling mediated by Mac-1, but not LFA-1...
  11. pmc T-lineage cells require the thymus but not VDJ recombination to produce IL-17A and regulate granulopoiesis in vivo
    Emily Smith
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Immunol 183:5685-93. 2009
    ..Adoptively transferred Rag1(-/-) or WT thymocytes correct neutrophil counts in neutropenic nude mice. We conclude that the development of IL-17A-producing T-lineage cells requires an intact thymic epithelium, but not V(D)J recombination...
  12. pmc Blockade of interleukin-17A results in reduced atherosclerosis in apolipoprotein E-deficient mice
    Emily Smith
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, USA
    Circulation 121:1746-55. 2010
    ..Here, we tested the hypothesis that atherosclerosis-prone conditions induce the differentiation of IL-17A-producing T cells, which in turn promote atherosclerosis...
  13. pmc Distinct roles for talin-1 and kindlin-3 in LFA-1 extension and affinity regulation
    Craig T Lefort
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA
    Blood 119:4275-82. 2012
    ..We conclude that talin-1 and kindlin-3 serve distinct functions in LFA-1 activation...
  14. pmc Critical role of endothelial P-selectin glycoprotein ligand 1 in chronic murine ileitis
    Jesus Rivera-Nieves
    Digestive Health Center of Excellence, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Exp Med 203:907-17. 2006
    ..Thus, the multiple roles of PSGL-1 may explain why targeting this single adhesion molecule results in attenuation of chronic murine ileitis, a disease previously resistant to antiadhesion molecule strategies...
  15. pmc CD40 ligand promotes Mac-1 expression, leukocyte recruitment, and neointima formation after vascular injury
    Guohong Li
    Cardiovascular Division, Robert M Berne Cardiovascular Research Center, University of Virginia Health System, Charlottesville, VA, USA
    Am J Pathol 172:1141-52. 2008
    ....
  16. pmc IL-17A inhibits the expansion of IL-17A-producing T cells in mice through "short-loop" inhibition via IL-17 receptor
    Emily Smith
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Immunol 181:1357-64. 2008
    ..We conclude that IL-17A regulates blood neutrophil counts by inducing G-CSF production mainly in nonhemopoietic cells. IL-17A controls the expansion of IL-17A-producing Tn cell populations through IL-17R...
  17. pmc Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908 1394, USA
    J Clin Invest 116:3211-9. 2006
    ..The key findings were confirmed in a sepsis-induced model of ALI. These findings may translate into improved clinical treatments for ALI...
  18. ncbi request reprint Critical role of platelet P-selectin in the response to arterial injury in apolipoprotein-E-deficient mice
    David Manka
    Department of Biomedical Engineering, University of Virginia Health System, Charlottesville, VA 22908 0158, USA
    Arterioscler Thromb Vasc Biol 24:1124-9. 2004
    ..To define the mechanism, bone marrow transplantation (BMT) after lethal irradiation was used to generate apoE-/- chimeric mice deficient in platelet, but not endothelial, P-selectin...
  19. pmc Dynamic T cell-APC interactions sustain chronic inflammation in atherosclerosis
    Ekaterina K Koltsova
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology LIAI, La Jolla, California 92037, USA
    J Clin Invest 122:3114-26. 2012
    ....
  20. pmc Lymphocyte recruitment into the aortic wall before and during development of atherosclerosis is partially L-selectin dependent
    Elena Galkina
    Department of Biomedical Engineering, University of Virginia, Health Sciences Center, Charlottesville, VA 22908, USA
    J Exp Med 203:1273-82. 2006
    ..Atherosclerosis induces the recruitment of macrophages and dendritic cells that support antigen presentation...
  21. pmc Endothelial cell PECAM-1 promotes atherosclerotic lesions in areas of disturbed flow in ApoE-deficient mice
    Brian L Harry
    Departments of Biomedical Engineering, Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, USA
    Arterioscler Thromb Vasc Biol 28:2003-8. 2008
    ..The aim of this study was to determine the in vivo role of PECAM-1 in atherosclerosis...
  22. ncbi request reprint Phagocytosis of apoptotic neutrophils regulates granulopoiesis via IL-23 and IL-17
    Matthew A Stark
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Immunity 22:285-94. 2005
    ..Antibody blockade of the p40 subunit of IL-23 reduces neutrophil numbers in wild-type mice. These findings identify a major homeostatic mechanism for the regulation of neutrophil production in vivo...
  23. pmc Blocking p21-activated kinase reduces lipopolysaccharide-induced acute lung injury by preventing polymorphonuclear leukocyte infiltration
    Jörg Reutershan
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908 1394, USA
    Am J Respir Crit Care Med 175:1027-35. 2007
    ..p21-Activated kinase (PAK) is an effector of small GTPases and has been implicated in cell migration...
  24. pmc Neutrophil adhesion and activation under flow
    Alexander Zarbock
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Microcirculation 16:31-42. 2009
    ..This review provides an overview of signaling in rolling and adherent neutrophils...
  25. pmc Induction of vascular permeability: beta PIX and GIT1 scaffold the activation of extracellular signal-regulated kinase by PAK
    Rebecca Stockton
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Mol Biol Cell 18:2346-55. 2007
    ..We conclude that the PAK-PIX-GIT1 complex is critical for Erk-dependent myosin phosphorylation and vascular permeability...
  26. pmc The primary defect in experimental ileitis originates from a nonhematopoietic source
    Timothy S Olson
    Department of Molecular Physiology, University of Virginia Health System, Charlottesville, VA 22908, USA
    J Exp Med 203:541-52. 2006
    ..Decreased barrier function suggests that defects in the epithelium may represent the primary source of SAMP ileitis susceptibility...
  27. ncbi request reprint Leukocyte phosphoinositide-3 kinase {gamma} is required for chemokine-induced, sustained adhesion under flow in vivo
    David F Smith
    Department of Molecular Physiology and Biophysics, University of Virginia, Charlottesville, Virginia 22908, USA
    J Leukoc Biol 80:1491-9. 2006
    ..We conclude that the gamma isoform of PI-3K must be functional in leukocytes to allow efficient adhesion from rolling in response to chemokine stimulation...
  28. ncbi request reprint ICAM-1 and LFA-1 play critical roles in LPS-induced neutrophil recruitment into the alveolar space
    Abdul Basit
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, 22908, USA
    Am J Physiol Lung Cell Mol Physiol 291:L200-7. 2006
    ..We conclude that ICAM-1 and LFA-1 play critical roles in the recruitment of neutrophils into the alveolar space in aerosolized LPS-induced lung inflammation, and LFA-1 serves as a ligand of ICAM-1 in the lung...
  29. pmc Beta7 integrin deficiency suppresses B cell homing and attenuates chronic ileitis in SAMP1/YitFc mice
    Gezahegn Gorfu
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Immunol 185:5561-8. 2010
    ..Our findings suggest that β(7) integrins play an essential role in spontaneous chronic ileitis in vivo by promoting homing of disease-exacerbating B cells to MLNs and other intestinal tissues...
  30. pmc Critical role of endothelial CXCR2 in LPS-induced neutrophil migration into the lung
    Jörg Reutershan
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia 22908 1394, USA
    J Clin Invest 116:695-702. 2006
    ..Our data revealed what we believe to be a previously unrecognized role of endothelial and epithelial CXCR2 in LPS-induced PMN recruitment and lung injury...
  31. ncbi request reprint Antibody blockade of CCL25/CCR9 ameliorates early but not late chronic murine ileitis
    Jesus Rivera-Nieves
    Digestive Health Center of Excellence, Department of Internal Medicine, University of Virginia, Charlottesville, Virginia, USA
    Gastroenterology 131:1518-29. 2006
    ..Furthermore, although clinical trials to evaluate the efficacy of targeting the CCL25/CCR9 axis for the treatment of Crohn's disease are being conducted, preclinical data in animal models of IBD are lacking...
  32. pmc Arterial macrophages and regenerating endothelial cells express P-selectin in atherosclerosis-prone apolipoprotein E-deficient mice
    Guohong Li
    Department of Medicine, University of Virginia Health System, Charlottesville, 22908, USA
    Am J Pathol 167:1511-8. 2005
    ..These findings provide a potential new paradigm in macrophage-mediated vascular inflammation, atherosclerosis, and neointimal hyperplasia after arterial injury...
  33. ncbi request reprint Sphingosine-1 phosphate prevents monocyte/endothelial interactions in type 1 diabetic NOD mice through activation of the S1P1 receptor
    Angela M Whetzel
    Cardiovascular Research Center, University of Virginia, 415 Lane Rd, Charlottesville, VA 22908, USA
    Circ Res 99:731-9. 2006
    ..S1P may play an important role in the prevention of vascular complications of type 1 diabetes...
  34. ncbi request reprint CXCR6 promotes atherosclerosis by supporting T-cell homing, interferon-gamma production, and macrophage accumulation in the aortic wall
    Elena Galkina
    Department of Biomedical Engineering and Robert M Berne Cardiovascular Research Center, University of Virginia, Health Sciences Center, Charlottesville, VA 22908, USA
    Circulation 116:1801-11. 2007
    ....
  35. ncbi request reprint Critical role of macrophage 12/15-lipoxygenase for atherosclerosis in apolipoprotein E-deficient mice
    Yuqing Huo
    University of Virginia, Charlottesville, VA, USA
    Circulation 110:2024-31. 2004
    ..12/15-LO is expressed in a variety of cells, including vascular cells, adipocytes, macrophages, and cardiomyocytes. The purpose of this study was to determine which cellular source of 12/15-LO is important for atherosclerosis...
  36. pmc Rolling on E- or P-selectin induces the extended but not high-affinity conformation of LFA-1 in neutrophils
    Yoshihiro Kuwano
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, CA, USA
    Blood 116:617-24. 2010
    ..We conclude that human neutrophil rolling on E- or P-selectin induces the extended alpha(L)beta(2) integrin conformation through signaling triggered by PSGL-1 engagement...
  37. pmc P-selectin glycoprotein ligand-1 mediates L-selectin-dependent leukocyte rolling in venules
    Markus Sperandio
    University of Virginia Health System, Cardiovascular Research Center, MR5 Bldg, Rm 1013, P O Box 801 394, Charlottesville, VA 22908 1394, USA
    J Exp Med 197:1355-63. 2003
    ..L-selectin binding to PSGL-1 initiates tethering events that enable L-selectin-independent leukocyte-endothelial interactions. These findings provide a molecular mechanism for the inflammatory defects seen in L-selectin-deficient mice...
  38. ncbi request reprint Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin
    Michael L Smith
    Department of Biomedical Engineering, University of Virginia, Charlottesville, USA
    J Leukoc Biol 76:985-93. 2004
    ..The unexpected synergism between E- and P-selectins explains why neutrophil recruitment is enhanced when both selectins are expressed...
  39. ncbi request reprint T cell requirement for development of chronic ulcerative dermatitis in E- and P-selectin-deficient mice
    S Bradley Forlow
    Department of Biomedical Engineering, Cardiovascular Research Center, and Center for Comparative Medicine, University of Virginia, Charlottesville 22908, USA
    J Immunol 169:4797-804. 2002
    ..We conclude that a dysregulated immune response in E(-/-)P(-/-) mice causes disease development, but is not necessary for elevated neutrophil counts...
  40. pmc Neutrophilic granulocytes modulate invariant NKT cell function in mice and humans
    Gerhard Wingender
    Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Immunol 188:3000-8. 2012
    ..Our data reveal a novel regulatory axis whereby neutrophils reduce iNKT cell responses, which may be important in shaping the extent of inflammation...
  41. pmc Monocyte-endothelial cell interactions in the development of atherosclerosis
    Javier Mestas
    La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Trends Cardiovasc Med 18:228-32. 2008
    ..This review discusses the nature of these molecules and the mechanisms involved in the early steps of monocyte recruitment into atherosclerotic lesion sites within the vessel wall...
  42. pmc Mechanisms and consequences of neutrophil interaction with the endothelium
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia, USA
    Am J Pathol 172:1-7. 2008
    ..This Review is focused on the signaling events during the interaction of neutrophils with the endothelium...
  43. ncbi request reprint L-selectin, alpha 4 beta 1, and alpha 4 beta 7 integrins participate in CD4+ T cell recruitment to chronically inflamed small intestine
    Jesus Rivera-Nieves
    Digestive Health Center of Excellence, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    J Immunol 174:2343-52. 2005
    ..Thus, pathogenic CD4+ T cells not only use the physiologic alpha4beta7/MAdCAM-1 pathway, but alternatively engage alpha4beta1 and L-selectin to recirculate to the chronically inflamed small intestine...
  44. pmc CXCR2- and E-selectin-induced neutrophil arrest during inflammation in vivo
    Michael L Smith
    Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA
    J Exp Med 200:935-9. 2004
    ..These data show that E-selectin- and chemokine-mediated arrest mechanisms are overlapping in this model and identify CXCR2 as an important neutrophil arrest chemokine in vivo...
  45. ncbi request reprint Therapeutic anti-inflammatory effects of myeloid cell adenosine receptor A2a stimulation in lipopolysaccharide-induced lung injury
    Jörg Reutershan
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    J Immunol 179:1254-63. 2007
    ..These findings were confirmed in A2a(flox/flox) x LysM-cre mice. ATL202 was only effective when A2a was present on BM-derived cells. A2a agonists may be effective at curbing inflammatory lung tissue damage...
  46. ncbi request reprint Mycophenolic acid suppresses granulopoiesis by inhibition of interleukin-17 production
    Sibylle von Vietinghoff
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA
    Kidney Int 78:79-88. 2010
    ..Our study shows that mycophenolic acid suppresses neutrophil production by inhibiting IL-17 expression, suggesting that measurement of this interleukin might be useful in estimating the risk of neutropenia in clinical settings...
  47. pmc IL-17A controls IL-17F production and maintains blood neutrophil counts in mice
    Sibylle von Vietinghoff
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA
    J Immunol 183:865-73. 2009
    ..We conclude that IL-17A mediated suppression of IL-17F production and secretion requires IL-17RA and is relevant to maintain the normal set point of blood neutrophil counts in vivo...
  48. doi request reprint Chapter 11. Intravital microscopic investigation of leukocyte interactions with the blood vessel wall
    Klaus Ley
    La Jolla Institute for Allergy and Immunology, La Jolla, California, USA
    Methods Enzymol 445:255-79. 2008
    ..This chapter focuses on the application of these techniques to study leukocyte interactions with the vascular wall and molecular surfaces...
  49. pmc Increased cholesterol content in gammadelta (γδ) T lymphocytes differentially regulates their activation
    Hsin Yuan Cheng
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
    PLoS ONE 8:e63746. 2013
    ..Our data indicate that cholesterol metabolism is differentially regulated in γδ T cells. The high intracellular cholesterol content leads to enhanced TCR signaling and increases activation and proliferation of γδ T cells...
  50. pmc Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice
    Sibylle von Vietinghoff
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92122, USA
    J Immunol 185:670-8. 2010
    ..These neutrophil effects may limit the usefulness of global NF-kappaB inhibitors for the treatment of inflammatory diseases...
  51. pmc CXC chemokine ligand 4 induces a unique transcriptome in monocyte-derived macrophages
    Christian A Gleissner
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Immunol 184:4810-8. 2010
    ..We conclude that CXCL4 induces a unique macrophage transcriptome distinct from known macrophage types, defining a new macrophage differentiation that we propose to call M4...
  52. pmc CXCL4 downregulates the atheroprotective hemoglobin receptor CD163 in human macrophages
    Christian A Gleissner
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Circ Res 106:203-11. 2010
    ..CXCL4 is a platelet-derived chemokine that promotes macrophage differentiation from monocytes. Deletion of the PF4 gene that encodes CXCL4 reduces atherosclerotic lesions in ApoE(-/-) mice...
  53. ncbi request reprint IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice
    Emily Smith
    Robert M Berne Cardiovascular Research Center, Department of Biomedical Engineering, University of Virginia, Charlottesville 22908, USA
    J Immunol 179:8274-9. 2007
    ..Our results suggest a prominent role of IL-23 in the regulation of granulopoiesis and the prevalence of IL-17A-producing Tn cells...
  54. pmc Galphai2 is required for chemokine-induced neutrophil arrest
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908 1394, USA
    Blood 110:3773-9. 2007
    ..These data show that neutrophil Galpha(i2) is necessary for chemokine-induced arrest, which is relevant for neutrophil recruitment to sites of acute inflammation...
  55. ncbi request reprint Induction of dendritic cell-like phenotype in macrophages during foam cell formation
    Hyung Jun Cho
    Department of Public Health Sciences, University of Virginia, Charlottesville, Virginia 22908, USA
    Physiol Genomics 29:149-60. 2007
    ..Our findings suggest that LDL and oxLDL, but not mmLDL, induce a dendritic cell-like phenotype in macrophages, suggesting that these cells may be able to present antigens and support an immune response...
  56. ncbi request reprint Transient T cell accumulation in lymph nodes and sustained lymphopenia in mice treated with FTY720
    Margaret A Morris
    Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Eur J Immunol 35:3570-80. 2005
    ..Our data indicate that the commonly held belief that FTY720 blocks lymphocyte egress from lymph nodes cannot fully explain the lymphocyte dynamics observed with prolonged treatment...
  57. pmc Initiation of protein O glycosylation by the polypeptide GalNAcT-1 in vascular biology and humoral immunity
    Mari Tenno
    Howard Hughes Medical Institute, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Mol Cell Biol 27:8783-96. 2007
    ..These findings reveal that the initiation of protein O glycosylation by ppGalNAcT-1 provides a distinctive repertoire of advantageous functions that support vascular responses and humoral immunity...
  58. ncbi request reprint Severely reduced neutrophil adhesion and impaired host defense against fecal and commensal bacteria in CD18-/-P-selectin-/- double null mice
    Stephen Bradley Forlow
    Department of Biomedical Engineering, Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia, USA
    FASEB J 16:1488-96. 2002
    ..gt;90% in wild-type, CD62P-/-, and CD18-/- single mutants. Our data show that removing both P-selectin and CD18 integrins from mice leads to severe neutrophil recruitment defects and spontaneous pathology...
  59. ncbi request reprint Control of leukocyte rolling velocity in TNF-alpha-induced inflammation by LFA-1 and Mac-1
    Jessica L Dunne
    Department of Biomedical Engineering and Cardiovascular Research Center, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    Blood 99:336-41. 2002
    ..Thus, both LFA-1 and Mac-1 contribute to slowing down rolling leukocytes, although LFA-1 is more important than Mac-1 in efficiently inducing firm adhesion...
  60. ncbi request reprint Sequential recruitment of neutrophils into lung and bronchoalveolar lavage fluid in LPS-induced acute lung injury
    Jörg Reutershan
    Robert M Berne Cardiovascular Research Center, Department of Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA
    Am J Physiol Lung Cell Mol Physiol 289:L807-15. 2005
    ..These findings confirm LPS-induced migration of PMN into the lung. For the first time, distinct transmigration steps into the different lung compartments are characterized in vivo...
  61. ncbi request reprint Induction of LFA-1-dependent neutrophil rolling on ICAM-1 by engagement of E-selectin
    Betsy C Chesnutt
    Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA
    Microcirculation 13:99-109. 2006
    ..To study rolling of mouse neutrophils on E-selectin and ICAM-1 in an ex vivo flow chamber system...
  62. pmc Interleukin-17 signaling in inflammatory, Kupffer cells, and hepatic stellate cells exacerbates liver fibrosis in mice
    Fanli Meng
    Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, California, USA
    Gastroenterology 143:765-76.e1-3. 2012
    ..Interleukin (IL)-17 signaling has been implicated in lung and skin fibrosis. We examined the role of IL-17 signaling in the pathogenesis of liver fibrosis in mice...
  63. ncbi request reprint Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E
    Yuqing Huo
    Department of Biomedical Engineering and Cardiovascular Research Center, University of Virginia, Health Science Center, Charlottesville, Virginia, USA
    Nat Med 9:61-7. 2003
    ..This role of activated platelets in atherosclerosis is attributed to platelet P-selectin-mediated delivery of platelet-derived proinflammatory factors to monocytes/leukocytes and the vessel wall...
  64. pmc Upregulation of aldose reductase during foam cell formation as possible link among diabetes, hyperlipidemia, and atherosclerosis
    Christian A Gleissner
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle Drive, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 28:1137-43. 2008
    ..In diabetes, it is related to microvascular complications. We discovered AR expression in foam cells by gene chip screening and hypothesized that it may be relevant in atherosclerosis...
  65. ncbi request reprint Sphingosine kinase 2 is required for modulation of lymphocyte traffic by FTY720
    Yugesh Kharel
    Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 280:36865-72. 2005
    ..Our results emphasize the importance of SPHK2 expression in both lymphocytes and other tissues for immune modulation and drug metabolism...
  66. ncbi request reprint Viscosity-independent velocity of neutrophils rolling on p-selectin in vitro or in vivo
    Michael L Smith
    Department of Biomedical Engineering, University of Virginia, Health Science Center, Charlottesville, VA 22908, USA
    Microcirculation 9:523-36. 2002
    ..To determine whether selectin-mediated leukocyte-rolling velocity in inflamed venules in vivo is determined by wall shear rate (WSR) or by wall shear stress (WSS)...
  67. ncbi request reprint Platelet-neutrophil-interactions: linking hemostasis and inflammation
    Alexander Zarbock
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia 22908 1394, USA
    Blood Rev 21:99-111. 2007
    ..In addition, platelets secrete neutrophil and endothelial activators inducing production of inflammatory cytokines. Thus, platelets are important amplifiers of acute inflammation...
  68. ncbi request reprint Effects of native and modified low-density lipoproteins on monocyte recruitment in atherosclerosis
    Christian A Gleissner
    Department of Biomedical Engineering, Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA
    Hypertension 50:276-83. 2007
  69. ncbi request reprint Rosiglitazone reduces the accelerated neointima formation after arterial injury in a mouse injury model of type 2 diabetes
    J William Phillips
    Department of Medicine, University of Virginia Health System, Charlottesville, VA, USA
    Circulation 108:1994-9. 2003
    ..We additionally hypothesized that an insulin-sensitizing agent (rosiglitazone) would prevent the development of type 2 diabetes and reduce neointima formation after carotid wire injury measured at 28 days...
  70. pmc Homeostatic regulation of blood neutrophil counts
    Sibylle von Vietinghoff
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Immunol 181:5183-8. 2008
    ..One example is benign ethnic neutropenia, which is found in some African Americans. Reduced and elevated neutrophil counts, even within the normal range, are associated with excess all-cause mortality...
  71. ncbi request reprint Leukocyte recruitment and vascular injury in diabetic nephropathy
    Elena Galkina
    Department of Biomedical Engineering and Robert M Berne Cardiovascular Research Center, University of Virginia, PO Box 801394, Charlottesville, VA 22908, USA
    J Am Soc Nephrol 17:368-77. 2006
    ..This review focuses on the molecular mechanisms of leukocyte recruitment into the diabetic kidney and the involvement of immigrated immune cells in the damage to renal tissues...
  72. ncbi request reprint Integration of inflammatory signals by rolling neutrophils
    Klaus Ley
    Cardiovascular Research Center, University of Virginia Health System, Cardiovascular Research Center, Charlottesville, VA 22908 1394, USA
    Immunol Rev 186:8-18. 2002
    ..This review provides a conceptual framework for neutrophil recruitment from the flowing blood...
  73. pmc Opening the flood-gates: how neutrophil-endothelial interactions regulate permeability
    Matthew R Distasi
    La Jolla Institute of Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA
    Trends Immunol 30:547-56. 2009
    ..Neutrophils also generate reactive oxygen species that induce vascular leakage. This review focuses on the mechanisms of neutrophil-mediated leakage...
  74. pmc Biomechanics of leukocyte rolling
    Prithu Sundd
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Biorheology 48:1-35. 2011
    ..Thus, improvement beyond the current understanding requires in silico models that can predict both cellular and microvillus deformation and experiments that allow measurement of forces acting on individual microvilli and tethers...
  75. pmc Transcriptional regulation of mouse L-selectin
    Xitong Dang
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    Biochim Biophys Acta 1789:146-52. 2009
    ..Silencing the expression of Sp1 by siRNA significantly decreased sell promoter activity in EL4 cells. We conclude that sell transcription is regulated by Mzf1, Klf2, Sp1, Ets1, and Irf1...
  76. ncbi request reprint Tracking leukocytes in vivo with shape and size constrained active contours
    Nilanjan Ray
    Department of Electrical and Computer Engineering, The University of Virginia, Charlottesville, VA 22904, USA
    IEEE Trans Med Imaging 21:1222-35. 2002
    ..For screening and drug validation, the tracker shows promise as an automated data collection tool...
  77. ncbi request reprint Automatic tracking of rolling leukocytes in vivo
    Scott T Acton
    Department of Electrical and Computer Engineering, University of Virginia, Charlottesville, Virginia 22904, USA
    Microvasc Res 63:139-48. 2002
    ..The automated tracking system allows one, for the first time, to reliably track rolling leukocytes in vivo, thus eliminating possible investigator bias and increasing throughput...
  78. ncbi request reprint L-selectin is required for fMLP- but not C5a-induced margination of neutrophils in pulmonary circulation
    Timothy S Olson
    Department of Molecular Physiology and Medical Scientist Training Program, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    Am J Physiol Regul Integr Comp Physiol 282:R1245-52. 2002
    ..We conclude that chemoattractant-induced PMN margination in the pulmonary circulation can occur by two separate mechanisms, one of which requires L-selectin...
  79. ncbi request reprint Oxidative modification of leukocyte adhesion
    Klaus Ley
    Cardiovascular Research Center and Departments of Biomedical Engineering, Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Immunity 22:5-7. 2005
    ..2005). Their data demonstrate the importance of oxidative modification of (unknown) adhesion molecules in regulating inflammation and lymphocyte homing...
  80. ncbi request reprint Hold on to your endothelium: postarrest steps of the leukocyte adhesion cascade
    Klaus Ley
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia 22908, USA
    Immunity 25:185-7. 2006
  81. pmc Sulfated sugars for rolling lymphocytes
    Klaus Ley
    Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA
    J Exp Med 198:1285-8. 2003
  82. pmc Event-tracking model of adhesion identifies load-bearing bonds in rolling leukocytes
    Maria K Pospieszalska
    Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA
    Microcirculation 16:115-30. 2009
    ..The aims of this study were to develop an efficient, high temporal resolution model for direct simulation of leukocyte rolling and conduct a study of load-bearing bonds using the model...
  83. ncbi request reprint Local-pooled-error test for identifying differentially expressed genes with a small number of replicated microarrays
    Nitin Jain
    Hospital West Complex, Rm 3181, P O Box 800717, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Bioinformatics 19:1945-51. 2003
    ....
  84. ncbi request reprint Cells on the run: shear-regulated integrin activation in leukocyte rolling and arrest on endothelial cells
    Ronen Alon
    Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel
    Curr Opin Cell Biol 20:525-32. 2008
    ..Different leukocyte subsets appear to use different modalities of integrin activation during rolling and arrest at distinct endothelial sites...
  85. ncbi request reprint Analysis of leukocyte rolling in vivo and in vitro
    Markus Sperandio
    Children s Hospital, Division of Neonatology, University of Heidelberg, Germany
    Methods Enzymol 416:346-71. 2006
    ....
  86. pmc Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation
    David Frommhold
    Children s Hospital, University of Heidelberg, 69120 Heidelberg, Germany
    J Exp Med 205:1435-46. 2008
    ..Collectively, we provide substantial evidence that sialylation by ST3Gal-IV significantly contributes to CXCR2-mediated leukocyte adhesion during inflammation in vivo...
  87. ncbi request reprint Targeted disruption of cd73/ecto-5'-nucleotidase alters thromboregulation and augments vascular inflammatory response
    Patrycja Koszalka
    Department of Cardiovascular Physiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany
    Circ Res 95:814-21. 2004
    ....
  88. ncbi request reprint Deposition of platelet RANTES triggering monocyte recruitment requires P-selectin and is involved in neointima formation after arterial injury
    Andreas Schober
    Department of Molecular Cardiovascular Research and Cardiology, Rheinisch Westfälische Technische Hochschule Aachen, Germany
    Circulation 106:1523-9. 2002
    ....
  89. ncbi request reprint CXCL4 in atherosclerosis: possible roles in monocyte arrest and macrophage foam cell formation
    Christian A Gleissner
    Thromb Haemost 98:917-8. 2007
  90. ncbi request reprint Signals for lymphocyte egress
    Klaus Ley
    Nat Immunol 6:1215-6. 2005
  91. pmc Acute cardiovascular protective effects of corticosteroids are mediated by non-transcriptional activation of endothelial nitric oxide synthase
    Ali Hafezi-Moghadam
    The Center for Blood Research, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 8:473-9. 2002
    ..The rapid activation of eNOS by the non-nuclear actions of GR, therefore, represents an important cardiovascular protective effect of acute high-dose corticosteroid therapy...
  92. ncbi request reprint Double jeopardy: how soluble P-selectin activates leukocytes in peripheral arterial occlusive disease
    Elena Galkina
    Circ Res 98:12-4. 2006
  93. ncbi request reprint Macrophage differentiation to foam cells
    Pavel Shashkin
    University of Virginia Health System, Cardiovascular Research Center, MR5 Bldg Rm 1013, P O Box 801 394, Charlottesville, VA 22908, USA
    Curr Pharm Des 11:3061-72. 2005
    ..Selective inhibitors of lipoxygenases and leukotriene receptors could be useful in the treatment of atherosclerosis by preventing or reducing foam cell formation...
  94. ncbi request reprint Vascular protection by estrogen in ischemia-reperfusion injury requires endothelial nitric oxide synthase
    Alyson J Prorock
    Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville 22908, USA
    Am J Physiol Heart Circ Physiol 284:H133-40. 2003
    ....
  95. ncbi request reprint Leukocyte influx in atherosclerosis
    Elena Galkina
    Department of Biomedical Engineering and Robert M Berne Cardiovascular Research Center, University of Virginia, Health Sciences Center, Charlottesville, VA 22908, USA
    Curr Drug Targets 8:1239-48. 2007
    ..In this review we shall summarize what is known about leukocyte recruitment into the aortic wall during atherosclerosis with a focus on mouse model systems...
  96. ncbi request reprint Weird and weirder: how circulating chemokines coax neutrophils to the lung
    Klaus Ley
    Am J Physiol Lung Cell Mol Physiol 286:L463-4. 2004
  97. ncbi request reprint LFA-1 is required for retention of effector CD8 T cells in mouse lungs
    Jayant Thatte
    Department of Biomedical Engineering, Beirne B Carter Center for Immunology Research, University of Virginia Health Sciences Center, Charlottesville, USA
    Blood 101:4916-22. 2003
    ..These results demonstrate a crucial role for LFA-1 in retention of activated CD8 T cells in normal mouse lungs and in the progression of lethal injury in HA-Tg mice...
  98. ncbi request reprint The role of selectins in inflammation and disease
    Klaus Ley
    Cardiovascular Research Center, MR5 Building, Room 1013, University of Virginia, PO Box 801394, Charlottesville, VA 22908 1394, USA
    Trends Mol Med 9:263-8. 2003
    ..Further improvements in the efficiency, availability, specificity and pharmacokinetics of selectin inhibitors, and specialized application routes and schedules, hold promise for therapeutic indications...
  99. ncbi request reprint Arrest chemokines
    Klaus Ley
    Cardiovascular Research Center and Department of Biomedical Engineering University of Virginia Health System, Charlottesville, VA 22903, USA
    Microcirculation 10:289-95. 2003
    ..Remarkably, no arrest chemokine for neutrophils under inflammatory conditions has been found so far...
  100. pmc Vav GEFs are required for beta2 integrin-dependent functions of neutrophils
    M Angelica Martinez Gakidis
    Department of Cell Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
    J Cell Biol 166:273-82. 2004
    ..Thus, Vav proteins play an essential role coupling beta2 to Rho GTPases and regulating multiple integrin-induced events important in leukocyte adhesion and phagocytosis...
  101. ncbi request reprint Selectins in T-cell recruitment to non-lymphoid tissues and sites of inflammation
    Klaus Ley
    Cardiovascular Research Center and Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Rev Immunol 4:325-35. 2004

Research Grants27

  1. MONOCYTE ADHESION TO ATHEROMA IN GENE TARGETED MICE
    Klaus Ley; Fiscal Year: 2009
    ..The proposed work may enable the design of interventions into the chemokine network to stabilize atherosclerotic plaques by reducing their content of inflammatory cells. ..
  2. MONOCYTE ADHESION TO ATHEROMA IN GENE TARGETED MICE
    Klaus Ley; Fiscal Year: 2007
    ..The proposed work may enable the design of interventions into the chemokine network to stabilize atherosclerotic plaques by reducing their content of inflammatory cells. ..
  3. MONOCYTE ADHESION TO ATHEROMA IN GENE TARGETED MICE
    Klaus Ley; Fiscal Year: 2006
    ..The proposed work may enable the design of interventions into the chemokine network to stabilize atherosclerotic plaques by reducing their content of inflammatory cells. ..
  4. Monocyte Adhesion to Atheroma in Gene Targeted
    Klaus Ley; Fiscal Year: 2005
    ..These studies are designed to (1) provide insight into the molecular mechanisms of monocyte and platelet recruitment to atherosclerotic lesions and (2) test their relevance in an established model of arterial injury in apoE-/- mice. ..
  5. BIOMECHANICS OF LEUKOCYTE ADHESION MOLECULES
    Klaus Ley; Fiscal Year: 2002
    ..Milestones for the following years are listed in the timeline. ..
  6. MONOCYTE ADHESION TO ATHEROMA IN GENE TARGETED MICE
    Klaus Ley; Fiscal Year: 2002
    ..These studies are designed to (1) provide insight into the molecular mechanisms of monocyte and platelet recruitment to atherosclerotic lesions and (2) test their relevance in an established model of arterial injury in apoE-/- mice. ..
  7. BIOMECHANICS OF LEUKOCYTE ADHESION MOLECULES
    Klaus Ley; Fiscal Year: 2001
    ..Milestones for the following years are listed in the timeline. ..
  8. SELECTIN MEDIATED LEUKOCYTE ADHESION IN VIVO
    Klaus Ley; Fiscal Year: 2001
    ..The knowledge gained will improve the understanding of the inflammatory process and provide significant information pertinent to the development of potential anti-inflammatory therapies for future clinical use in patients. ..
  9. MONOCYTE ADHESION TO ATHEROMA IN GENE TARGETED MICE
    Klaus Ley; Fiscal Year: 1999
    ..Studies such as this can provide a rational basis for the development of potential therapies targeting adhesion molecules. ..
  10. MONOCYTE ADHESION TO ATHEROMA IN GENE TARGETED MICE
    Klaus Ley; Fiscal Year: 2007
    ..The proposed work may enable the design of interventions into the chemokine network to stabilize atherosclerotic plaques by reducing their content of inflammatory cells. ..
  11. BIOMECHANICS OF LEUKOCYTE ADHESION MOLECULES
    Klaus Ley; Fiscal Year: 1999
    ..Milestones for the following years are listed in the timeline. ..
  12. BIOMECHANICS OF LEUKOCYTE ADHESION MOLECULES
    Klaus Ley; Fiscal Year: 2000
    ..Milestones for the following years are listed in the timeline. ..
  13. Monocyte Adhesion to Atheroma in Gene Targeted
    Klaus Ley; Fiscal Year: 2004
    ..These studies are designed to (1) provide insight into the molecular mechanisms of monocyte and platelet recruitment to atherosclerotic lesions and (2) test their relevance in an established model of arterial injury in apoE-/- mice. ..
  14. Biomechanics of Leukocytes and Molecular Imaging Agents
    Klaus Ley; Fiscal Year: 2006
    ..Since ultrasound is the most economical of the modern imaging modalities, cost savings in health care can also be expected from the proposed research. ..
  15. BIOMECHANICS OF LEUKOCYTE ADHESION MOLECULES
    Klaus Ley; Fiscal Year: 2003
    ..Milestones for the following years are listed in the timeline. ..
  16. Monocyte Adhesion to Atheroma in Gene Targeted
    Klaus Ley; Fiscal Year: 2003
    ..These studies are designed to (1) provide insight into the molecular mechanisms of monocyte and platelet recruitment to atherosclerotic lesions and (2) test their relevance in an established model of arterial injury in apoE-/- mice. ..
  17. SELECTIN MEDIATED LEUKOCYTE ADHESION IN VIVO
    Klaus Ley; Fiscal Year: 2002
    ..The knowledge gained will improve the understanding of the inflammatory process and provide significant information pertinent to the development of potential anti-inflammatory therapies for future clinical use in patients. ..
  18. SELECTIN MEDIATED LEUKOCYTE ADHESION IN VIVO
    Klaus Ley; Fiscal Year: 1999
    ..The knowledge gained will improve the understanding of the inflammatory process and provide significant information pertinent to the development of potential anti-inflammatory therapies for future clinical use in patients. ..
  19. SELECTIN MEDIATED LEUKOCYTE ADHESION IN VIVO
    Klaus Ley; Fiscal Year: 2000
    ..The knowledge gained will improve the understanding of the inflammatory process and provide significant information pertinent to the development of potential anti-inflammatory therapies for future clinical use in patients. ..
  20. MONOCYTE ADHESION TO ATHEROMA IN GENE TARGETED MICE
    Klaus Ley; Fiscal Year: 2000
    ..Studies such as this can provide a rational basis for the development of potential therapies targeting adhesion molecules. ..