Research Topics
Genomes and Genes
| Yuko KawakamiSummaryAffiliation: La Jolla Institute for Allergy and Immunology Country: USA Publications
Research Grants
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Detail Information
Publications
An improved mouse model of atopic dermatitis and suppression of skin lesions by an inhibitor of Tec family kinasesYuko Kawakami
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
Allergol Int 56:403-9. 2007..We attempted to develop an improved method to induce an animal model of atopic dermatitis and to use it to evaluate the efficacy of a Tec family kinase inhibitor...
Inhibition of NK cell activity by IL-17 allows vaccinia virus to induce severe skin lesions in a mouse model of eczema vaccinatumYuko Kawakami
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
J Exp Med 206:1219-25. 2009..Given low NK cell activities and increased IL-17 expression in atopic dermatitis patients, these results can explain the increased susceptibility of atopic dermatitis patients to eczema vaccinatum...- A Ras activation pathway dependent on Syk phosphorylation of protein kinase CYuko Kawakami
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Proc Natl Acad Sci U S A 100:9470-5. 2003..By recruiting the Grb-2/Sos complex to the plasma membrane, these conventional PKC isoforms contribute to the full activation of the Ras/extracellular signal-regulated kinase signaling pathway in FcepsilonRI-stimulated mast cells... - The Src family kinase Hck regulates mast cell activation by suppressing an inhibitory Src family kinase LynHong Hong
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, CA 92037, USA
Blood 110:2511-9. 2007..Thus, this study has revealed a hierarchical regulation among SFK members to fine-tune mast cell activation... - Positive and negative regulation of mast cell activation by Lyn via the FcepsilonRIWenbin Xiao
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
J Immunol 175:6885-92. 2005..Furthermore, Lyn-mediated negative regulation was shown to be exerted via the FcepsilonRI beta-ITAM... - Regulation of highly cytokinergic IgE-induced mast cell adhesion by Src, Syk, Tec, and protein kinase C family kinasesJiro Kitaura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
J Immunol 174:4495-504. 2005..Thus, binding of HC IgEs to Fc epsilon RI induces adhesion of mast cells to fibronectin by modulating cellular activation signals in a unique fashion... - Early divergence of Fc epsilon receptor I signals for receptor up-regulation and internalization from degranulation, cytokine production, and survivalJiro Kitaura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
J Immunol 173:4317-23. 2004..Collectively, signals emanated from IgE-bound FcepsilonRI for receptor up-regulation and internalization are shown to diverge at the receptor or receptor-proximal levels from those for other biological outcomes... - Phospholipase C-β3 regulates FcɛRI-mediated mast cell activation by recruiting the protein phosphatase SHP-1Wenbin Xiao
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
Immunity 34:893-904. 2011..Thus, we have defined a PLC-β3- and SHP-1-mediated signaling pathway for FcɛRI-mediated cytokine production... - Protein kinase C betaII regulates Akt phosphorylation on Ser-473 in a cell type- and stimulus-specific fashionYuko Kawakami
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
J Biol Chem 279:47720-5. 2004..Therefore, PKCbetaII appears to work as a cell type- and stimulus-specific PDK2... - Lyn- and PLC-beta3-dependent regulation of SHP-1 phosphorylation controls Stat5 activity and myelomonocytic leukemia-like diseaseWenbin Xiao
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
Blood 116:6003-13. 2010..Therefore, we identify a novel Lyn/PLC-β3-mediated regulatory mechanism of SHP-1 and Stat5 activities... - A minor catalytic activity of Src family kinases is sufficient for maximal activation of mast cells via the high-affinity IgE receptorMichael Poderycki
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
J Immunol 184:84-93. 2010..Furthermore, the data suggest that both positive and negative regulatory functions of Lyn are predominantly carried out by its catalytic activity and not an adaptor function... - Dysregulation of Src family kinases in mast cells from epilepsy-resistant ASK versus epilepsy-prone EL miceJiro Kitaura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
J Immunol 178:455-62. 2007.... - Regulation of myeloproliferation and M2 macrophage programming in mice by Lyn/Hck, SHIP, and Stat5Wenbin Xiao
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA
J Clin Invest 118:924-34. 2008..Thus, this study has defined a myeloproliferative transformation-sensitive signaling pathway, composed of Lyn/Hck, SHIP, autocrine/paracrine cytokines, and Stat5, that regulates HSC differentiation and M2 macrophage programming... - Pivotal advance: IgE accelerates in vitro development of mast cells and modifies their phenotypeJun ichi Kashiwakura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA
J Leukoc Biol 84:357-67. 2008..Therefore, our results suggest that monomeric IgEs, especially HC IgEs, not only promote mast cell development but also modulate the mast cell phenotype... - Polyclonal IgE induces mast cell survival and cytokine productionJun ichi Kashiwakura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, CA, USA
Allergol Int 58:411-9. 2009..However, no studies have been performed on the effects of polyclonal IgE molecules. Here, we tested whether polyclonal mouse and human IgE molecules affect survival and cytokine production in mast cells... - Short Stat5-interacting peptide derived from phospholipase C-β3 inhibits hematopoietic cell proliferation and myeloid differentiationHiroki Yasudo
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
PLoS ONE 6:e24995. 2011.... - Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5Wenbin Xiao
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
Cancer Cell 16:161-71. 2009..The same mechanism for malignant transformation seems to be operative in other human lymphoid and myeloid malignancies. Thus, PLC-beta3 is likely a tumor suppressor... - Costimulation of mast cells by 4-1BB, a member of the tumor necrosis factor receptor superfamily, with the high-affinity IgE receptorHajime Nishimoto
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Dr, San Diego, CA 92121, USA
Blood 106:4241-8. 2005..Therefore, FcepsilonRI-inducible 4-1BB plays a costimulatory function together with FcepsilonRI stimulation... - IgE regulation of mast cell survival and functionToshiaki Kawakami
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, California 92121, USA
Novartis Found Symp 271:100-7; discussion 108-14, 145-51. 2005..Consistent with receptor aggregation induced by monomeric IgE, other means of receptor aggregation, e.g. IgE+antigen and IgE+anti-IgE, can also induce survival and other events in unique ranges of stimulation intensity... - IgE- and IgE+Ag-mediated mast cell migration in an autocrine/paracrine fashionJiro Kitaura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 10 355 Science Center Dr, San Diego, CA 92121, USA
Blood 105:3222-9. 2005..In mouse experiments, naive mast cells are attracted to IgE, and IgE-sensitized mast cells are attracted to antigen. Therefore, IgE and antigen are implicated in mast cell accumulation at allergic tissue sites with local high IgE levels... - Mast cells in atopic dermatitisToshiaki Kawakami
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
Curr Opin Immunol 21:666-78. 2009..Numerous animal models have been used to study this epidemic disease. Here we review the recent progress to synthesize our current understanding of this disease and potential mechanisms for a mast cell's role in the disease... - Histamine-releasing factor has a proinflammatory role in mouse models of asthma and allergyJun chi Kashiwakura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA
J Clin Invest 122:218-28. 2012..These results indicate that HRF has a proinflammatory role in asthma and skin immediate hypersensitivity, leading us to suggest HRF as a potential therapeutic target... - Regulation of dendritic cell maturation and function by Bruton's tyrosine kinase via IL-10 and Stat3Yuko Kawakami
Divisions of Cell Biology and Molecular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Proc Natl Acad Sci U S A 103:153-8. 2006..These negative regulatory functions of Btk in dendritic cells appear to be mediated mainly through autocrine secretion of IL-10 and subsequent activation of Stat3... - JNK1 controls mast cell degranulation and IL-1{beta} production in inflammatory arthritisMonica Guma
Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology and Pathology, Division of Rheumatology, School of Medicine, University of California at San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 107:22122-7. 2010..Hence, JNK1 controls mast cell degranulation and FcγR-triggered IL-1β production, in addition to regulating cytokine and matrix metalloproteinase biosynthesis, and is an attractive therapeutic target in inflammatory arthritis... - Protective murine and human monoclonal antibodies against eczema vaccinatumYoshiaki Tomimori
Division of Cell Biology, La Jolla Institute for Allergy and Immunology LIAI, La Jolla, CA, USA
Antivir Ther 16:67-75. 2011..Eczema vaccinatum is the most common severe pathology associated with smallpox vaccination (vaccinia virus), occurring at high rates among individuals with a previous history of atopic dermatitis (atopic eczema)... - Techniques to study Fc epsilonRI signalingYuko Kawakami
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA, USA
Methods Mol Biol 315:175-89. 2006..As signal transduction is highly dependent on protein-protein interactions, we will describe experimental details of co-immunoprecipitation methods that are used to confirm such interactions... 
Murine bone marrow-derived mast cells express chemoattractant receptor-homologous molecule expressed on T-helper class 2 cells (CRTh2)Stefen A Boehme
Actimis Pharmaceuticals, Inc, 10835 Road to the Cure, San Diego, CA 92121, USA
Int Immunol 21:621-32. 2009..Both these processes were inhibited by the CRT(h)2 antagonist. These results raise the possibility that the functional consequences of the PGD(2)-CRT(h)2 interaction on mast cells may be relevant in allergic inflammation...- [Regulation of mast cell survival and activation by monomeric IgE]Toshiaki Kawakami
La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
Arerugi 53:557-61. 2004 - Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcepsilonRIJiro Kitaura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Proc Natl Acad Sci U S A 100:12911-6. 2003..In hybridoma-transplanted mice, mucosal mast cell numbers correlate with serum IgE levels. Therefore, survival effects of IgE could contribute to the pathogenesis of allergic disease... - Most Highly Cytokinergic IgEs Have Polyreactivity to AutoantigensJun ichi Kashiwakura
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA Division of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, Nihon University Graduate School of Medical Science, Itabashi ku, Tokyo, Japan
Allergy Asthma Immunol Res 4:332-40. 2012..In this study, we investigated whether IgEs recognize autoantigens and whether IgEs' binding of autoantigens correlates with difference s in HC versus PC properties... - A novel role for the transcription factor HIF-1α in the formation of mast cell extracellular trapsKatja Branitzki-Heinemann
Department of Pediatrics, University of California San Diego, La Jolla, CA 92093, USA
Biochem J 446:159-63. 2012.... - Protein kinase C beta (PKC beta): normal functions and diseasesToshiaki Kawakami
Division of Allergy, La Jolla Institute for Allergy and Immunology, Science Center Drive, San Diego, California 92121, USA
J Biochem 132:677-82. 2002..Their involvement in disorders due to pertubations in these signaling systems, i.e., immunodeficiencies and diabetes, is also reviewed. Finally, patterns of PKC action in these and other biologic systems are discussed... - Fer and Fps/Fes participate in a Lyn-dependent pathway from FcepsilonRI to platelet-endothelial cell adhesion molecule 1 to limit mast cell activationChristian M Udell
Department of Biochemistry, Queen s University, Kingston, Ontario, Canada
J Biol Chem 281:20949-57. 2006..Thus, Lyn and Fer/Fps kinases cooperate to phosphorylate Pecam-1 and activate Shp1/Shp2 phosphatases that function in part to limit mast cell activation... - Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cellsHidetoshi Kumagai
Division of Hematopoietic Factors, The Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, 6 1 Shirokanedai 4 chome, Minato ku, Tokyo 108 8639, Japan
Biochem Biophys Res Commun 307:719-29. 2003..These findings suggest that a new pair of ITIM/ITAM-bearing receptors, LMIR1 and 2, regulate mast cell-mediated inflammatory responses through yet to be defined ligand(s)... - PKC-beta controls I kappa B kinase lipid raft recruitment and activation in response to BCR signalingThomas T Su
The Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
Nat Immunol 3:780-6. 2002..Together, these data define an essential role for PKC-beta in BCR survival signaling and highlight PKC-beta as a key therapeutic target for B-lineage malignancies... - The Tyr-kinase inhibitor AG879, that blocks the ETK-PAK1 interaction, suppresses the RAS-induced PAK1 activation and malignant transformationHong He
Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville/Melbourne, Australia
Cancer Biol Ther 3:96-101. 2004.... - O-methylated catechins from tea leaves inhibit multiple protein kinases in mast cellsMari Maeda-Yamamoto
National Institute of Vegetable and Tea Science, National Agriculture Research Organization, and School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
J Immunol 172:4486-92. 2004..These observations for the first time have revealed the molecular mechanisms of antiallergic effects of tea-derived catechins... - Intracellular mechanisms and receptor types for endothelin-1-induced positive and negative inotropy in mouse ventricular myocardiumIyuki Namekata
Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Miyama 2 2 1 Funabashi, Chiba, 274 8510, Japan
Naunyn Schmiedebergs Arch Pharmacol 376:385-95. 2008.... - Dietary intake, neutrophil fatty acid profile, serum antioxidant vitamins and oxygen radical absorbance capacity in patients with ulcerative colitisYuko Kawakami
Department of Human Nutrition, Chugokugakuen University, Okayama 701 0197, Japan
J Nutr Sci Vitaminol (Tokyo) 53:153-9. 2007..A diet restricting the intake of linoleic acid and supplemented with eicosapentaenoic acid and antioxidative vitamins may be recommendable for the nutritional management of ulcerative colitis patients... - Clinical characteristics of patients with cryptogenic liver cirrhosis in Okinawa, JapanHiroshi Sakugawa
First Department of Internal Medicine, Faculty of Medicine, School of Medicine, University of the Ryukyus, Okinawa, Japan
Hepatogastroenterology 50:2005-8. 2003..The aims of the present study were to determine the prevalence of patients with cryptogenic liver cirrhosis, and to clarify the clinical characteristics of these patients, with special focus on obesity and type 2 diabetes mellitus... - Role of galectin-3 in mast cell functions: galectin-3-deficient mast cells exhibit impaired mediator release and defective JNK expressionHuan Yuan Chen
Department of Dermatology, School of Medicine, University of California Davis, Sacramento, CA 95817, USA
J Immunol 177:4991-7. 2006..All together, these results point to an important role of galectin-3 in mast cell biology... - Quantitative time-resolved phosphoproteomic analysis of mast cell signalingLulu Cao
Department of Chemistry, Brown University, Providence, RI 02912, USA
J Immunol 179:5864-76. 2007....
Research Grants
- Tec inhibitors- A new therapeutic for allergic diseasesYuko Kawakami; Fiscal Year: 2003..Since we have a screening method for searching for Tec inhibitors whose usefulness has been proven, we will also expand our search for better compounds. ..