Michael A Model
Affiliation: Kent State University
- Intensity calibration of a laser scanning confocal microscope based on concentrated dyesMichael A Model
Department of Biological Sciences, Kent State University, Kent, Ohio, USA
Anal Quant Cytol Histol 28:253-61. 2006..To find water-soluble fluorescent dyes with absorption in various regions of the spectrum and investigate their utility as standards for laser scanning confocal microscopy...
- Measurement of the absorption of concentrated dyes and their use for quantitative imaging of surface topographyM A Model
Department of Biological Sciences, Cunningham Hall, Kent State University, Kent, OH 44242, USA
J Microsc 231:156-67. 2008..At the same time, the interference pattern in the reflected image allows measurement of the refractive index of the dye...
- 3D deconvolution of spherically aberrated images using commercial softwareM A Model
Department of Biological Sciences, Kent State University, Kent, OH 44242, U S A
J Microsc 241:94-100. 2011....
- Measurement of wheat germ agglutinin binding with a fluorescence microscopeMichael A Model
Department of Biological Sciences, Kent State University, Kent, Ohio 44242, USA
Cytometry A 75:874-81. 2009..For probes whose fluorescent properties are unaffected by binding, quantification of staining in true molecular units should be possible...
- Thickness profiling of formaldehyde-fixed cells by transmission-through-dye microscopyMariana Pelts
Department of Biological Sciences, Kent State University, Kent, OH, USA
Biotechniques 50:389-96. 2011..Quantification of cell thickness and volume on fixed cells is also possible during the early stages of fixation; this can be useful, for example, for measuring volume kinetics following rapid introduction of a stimulus...
- Effect of modeled reduced gravity conditions on bacterial morphology and physiologyRaja Vukanti
Department of Biological Sciences, Kent State University, PO Box 5190, Kent, OH 44242, USA
BMC Microbiol 12:4. 2012..The parameters selected have not been studied in E. coli and S. aureus under MRG conditions and provide critical information about bacterial viability and potential for population growth...
- Measurement of bacterial volume by transmission-through-dye imagingSuzanne L Lababidi
Department of Biological Sciences, Kent State University, Kent, OH 44242, USA
J Microbiol Methods 87:375-7. 2011..The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip...
- Intensity calibration and shading correction for fluorescence microscopesMichael A Model
Kent State University, Kent, Ohio, USA
Curr Protoc Cytom . 2006..The technique has a number of practical features that make it inexpensive, reproducible, and straightforward. Descriptions are given for both wide-field and confocal scanning fluorescence microscopy...
- Measurement of the thickness and volume of adherent cells using transmission-through-dye microscopyJennifer L Gregg
School of Biomedical Sciences, Kent State University, Kent, OH 44242, USA
Pflugers Arch 460:1097-104. 2010..To show possible applications of this method, we investigated the kinetics of the cell volume response to a hypotonic buffer and to the apoptotic agents staurosporine and ionomycin...
- Difference between mean and threshold as a measure of intensity in confocal microscopy imagesMichael A Model
Anal Quant Cytol Histol 28:341-3. 2006