L Takemoto

Summary

Affiliation: Kansas State University
Country: USA

Publications

  1. pmc Morphological characterization of the Alpha A- and Alpha B-crystallin double knockout mouse lens
    Daniel L Boyle
    Kansas State University, Division of Biology, Ackert Hall, Manhattan, KS, USA
    BMC Ophthalmol 3:3. 2003
  2. pmc Protein-protein interactions and lens transparency
    Larry Takemoto
    Division of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506, USA
    Exp Eye Res 87:496-501. 2008
  3. pmc Age-dependent association of gamma-crystallins with aged alpha-crystallins from old bovine lens
    Larry Takemoto
    Division of Biology, Kansas State University, Manhattan, KS, USA
    Mol Vis 14:970-4. 2008
  4. ncbi request reprint Decreased association of aged alpha crystallins with gamma crystallins
    Larry J Takemoto
    Department of Biology, Ackert Hall, Kansas State University, Manhattan, 66506, USA
    Exp Eye Res 83:793-7. 2006
  5. ncbi request reprint Deamidation of Asn-143 of gamma S crystallin from protein aggregates of the human lens
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas, USA
    Curr Eye Res 22:148-53. 2001
  6. ncbi request reprint Mechanism of asparagine deamidation during human senile cataractogenesis
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506, USA
    Exp Eye Res 72:559-63. 2001
  7. ncbi request reprint Increased deamidation of asparagine during human senile cataractogenesis
    L Takemoto
    Division of Biology, Kansas State University, Manhattan, KS
    Mol Vis 6:164-8. 2000
  8. ncbi request reprint Specific glutamine and asparagine residues of gamma-S crystallin are resistant to in vivo deamidation
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 275:26109-12. 2000
  9. ncbi request reprint Increased cleavage of the c-terminal serine from alpha-A crystallin present in the high molecular weight aggregate fraction from human and bovine lenses
    L Takemoto
    Kansas State University, Division of Biology, Manhattan 66506, USA
    Curr Eye Res 19:450-5. 1999
  10. ncbi request reprint Deamidation of alpha-A crystallin from nuclei of cataractous and normal human lenses
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506, USA
    Mol Vis 5:2. 1999

Research Grants

  1. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 2001
  2. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 2006
  3. LENS MEMBRANE IN RELATION TO CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 1980
  4. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 1993
  5. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 1991
  6. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry J Takemoto; Fiscal Year: 2010

Collaborators

Detail Information

Publications20

  1. pmc Morphological characterization of the Alpha A- and Alpha B-crystallin double knockout mouse lens
    Daniel L Boyle
    Kansas State University, Division of Biology, Ackert Hall, Manhattan, KS, USA
    BMC Ophthalmol 3:3. 2003
    ..The purpose of this study was to characterize gross lenticular morphology in normal mice and mice with the targeted disruption of alpha A- and alpha B-crystallin genes (alpha A/BKO)...
  2. pmc Protein-protein interactions and lens transparency
    Larry Takemoto
    Division of Biology, Kansas State University, Ackert Hall, Manhattan, KS 66506, USA
    Exp Eye Res 87:496-501. 2008
    ..Based upon this premise, model systems will be described that will test the validity of the theory...
  3. pmc Age-dependent association of gamma-crystallins with aged alpha-crystallins from old bovine lens
    Larry Takemoto
    Division of Biology, Kansas State University, Manhattan, KS, USA
    Mol Vis 14:970-4. 2008
    ..Previous theoretical and experimental studies have predicted that the loss of weak protein interactions between alpha- and gamma-crystallins could result in a decrease in the transparent properties of the aging lens...
  4. ncbi request reprint Decreased association of aged alpha crystallins with gamma crystallins
    Larry J Takemoto
    Department of Biology, Ackert Hall, Kansas State University, Manhattan, 66506, USA
    Exp Eye Res 83:793-7. 2006
    ....
  5. ncbi request reprint Deamidation of Asn-143 of gamma S crystallin from protein aggregates of the human lens
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas, USA
    Curr Eye Res 22:148-53. 2001
    ..Determine if gamma S crystallin is preferentially deamidated in the high molecular weight aggregate fraction of old human lenses...
  6. ncbi request reprint Mechanism of asparagine deamidation during human senile cataractogenesis
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506, USA
    Exp Eye Res 72:559-63. 2001
    ....
  7. ncbi request reprint Increased deamidation of asparagine during human senile cataractogenesis
    L Takemoto
    Division of Biology, Kansas State University, Manhattan, KS
    Mol Vis 6:164-8. 2000
    ..To identify and quantitate deamidation of a specific asparagine residue of gammaS-crystallin that preferentially undergoes deamidation during the process of human senile cataractogenesis...
  8. ncbi request reprint Specific glutamine and asparagine residues of gamma-S crystallin are resistant to in vivo deamidation
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 275:26109-12. 2000
    ....
  9. ncbi request reprint Increased cleavage of the c-terminal serine from alpha-A crystallin present in the high molecular weight aggregate fraction from human and bovine lenses
    L Takemoto
    Kansas State University, Division of Biology, Manhattan 66506, USA
    Curr Eye Res 19:450-5. 1999
    ....
  10. ncbi request reprint Deamidation of alpha-A crystallin from nuclei of cataractous and normal human lenses
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506, USA
    Mol Vis 5:2. 1999
    ..To quantitate the extent of deamidation of asparagine-101, glutamine-50, and glutamine-6 of alpha-A crystallin in the nucleus from human cataractous and normal lenses...
  11. ncbi request reprint Quantitation of asparagine-101 deamidation from alpha-A crystallin during aging of the human lens
    L J Takemoto
    Division of Biology, Kansas State University, Manhattan 66506, USA
    Curr Eye Res 17:247-50. 1998
    ..To quantitate deamidation of asparagine-101 from the alpha-A crystallin protein of human lenses of different ages...
  12. ncbi request reprint Deamidation of specific glutamine residues from alpha-A crystallin during aging of the human lens
    L Takemoto
    Division of Biology, Ackert Hall, Kansas State University, Manhattan USA
    Biochemistry 37:13681-5. 1998
    ..Together, these results are consistent with the hypothesis that resistance to age-dependent, nonenzymatic deamidation may be an important prerequisite for the stability of proteins in vivo...
  13. ncbi request reprint Finger-like projections of plasma membrane in the most senescent fiber cells of human lenses
    D L Boyle
    Division of Biology, Kansas State University, Manhattan 66506, USA
    Curr Eye Res 17:1118-23. 1998
    ..To corroborate the findings of finger-like membrane projections in monkey and baboon lenses, in human lens fiber cells...
  14. ncbi request reprint Differential phosphorylation of alpha-A crystallin in human lens of different age
    L J Takemoto
    Division of Biology, Kansas State University, Manhattan 66506, USA
    Exp Eye Res 62:499-504. 1996
    ....
  15. ncbi request reprint A possible role for alpha-crystallins in lens epithelial cell differentiation
    D L Boyle
    Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    Mol Vis 6:63-71. 2000
    ..To test the hypothesis that crystallin proteins may actively participate in the differentiation of lens epithelial cells into fiber cells...
  16. ncbi request reprint Role of albumin as a fatty acid carrier for biosynthesis of lens lipids
    J Sabah
    Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506, USA
    Exp Eye Res 80:31-6. 2005
    ..Together, the results strongly suggest that serum albumin in the aqueous humor and/or vitreous humor facilitates the translocation of long chain fatty acids into the lens, where they are used for the biosynthesis of lens lipids...
  17. ncbi request reprint Inhibition of gap junction activity through the release of the C1B domain of protein kinase Cgamma (PKCgamma) from 14-3-3: identification of PKCgamma-binding sites
    Thu Annelise Nguyen
    Department of Biochemistry and Division of Biology, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 279:52714-25. 2004
    ..This demonstrates that synthetic peptides can be used to exogenously regulate gap junctions...
  18. ncbi request reprint Differentially expressed genes in the lens of mimecan-null mice
    Elena S Tasheva
    Kansas State University, Division of Biology, Manhattan, KS 66506 4901, USA
    Mol Vis 10:403-16. 2004
    ..Here, we sought to identify gene expression changes in the lens that are associated with the absence of mimecan...
  19. ncbi request reprint Probing alpha-crystallin structure using chemical cross-linkers and mass spectrometry
    James J Peterson
    Kansas State University, Department of Biology, Manhattan 66506, USA
    Mol Vis 10:857-66. 2004
    ..We utilized mass spectrometry for 3 dimensional analysis (MS3D) to study the quaternary structural characteristics of this important lens protein and molecular chaperone...
  20. ncbi request reprint Transcytotic passage of albumin through lens epithelial cells
    Judith R Sabah
    Division of Biology, Kansas State University, Manhattan, Kansas, USA
    Invest Ophthalmol Vis Sci 48:1237-44. 2007
    ..To characterize the transcytotic passage of albumin through lens epithelial cells...

Research Grants29

  1. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 2001
    ....
  2. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 2006
    ....
  3. LENS MEMBRANE IN RELATION TO CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 1980
    ..Finally, both these approaches will be used to assess possible changes in membrane protein composition aad organization during human cataractogenesis...
  4. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 1993
    ....
  5. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry Takemoto; Fiscal Year: 1991
    ..transparent regions of the same cataractous lens. In this way, this study will correlate possible and observed changes in membrane function with the molecular mechanisms responsible for these changes...
  6. LENS MEMBRANE IN RELATION TO HUMAN CATARACTOGENESIS
    Larry J Takemoto; Fiscal Year: 2010
    ..Using these novel methods, we will be able to ascertain the existence of these interactions and their possible alterations during lens opacification. ..