Philip R Hardwidge
Affiliation: Kansas State University
Wu M, El Qaidi S, Hardwidge P. SseL Deubiquitinates RPS3 to Inhibit Its Nuclear Translocation. Pathogens. 2018;7: pubmed publisher
..Thus, Salmonella also encodes at least one T3SS effector that alters RPS3 activities in the host nucleus. ..
El Qaidi S, Wu M, Zhu C, Hardwidge P. Salmonella, E. coli, and Citrobacter Type III Secretion System Effector Proteins that Alter Host Innate Immunity. Adv Exp Med Biol. 2018;: pubmed publisher
..These effectors are interesting for structural and mechanistic reasons, as well as for their potential utility in being engineered to treat human autoimmune disorders associated with perturbations in NF-κB signaling. ..
Wu M, Hardwidge P. Hsp90 Interacts with the Bacterial Effector NleH1. Pathogens. 2018;7: pubmed publisher
..We determined that heat shock protein 90 (Hsp90) interacts with both IKKβ and NleH1 and that inhibiting Hsp90 activity reduces RPS3 nuclear translocation. ..
Wang G, Feuerbacher L, Hardwidge P. Influence of Intestinal Microbiota Transplantation and NleH Expression on Citrobacter rodentium Colonization of Mice. Pathogens. 2018;7: pubmed publisher
..rodentium infection as well as the benefit or detriment of expressing NleH to C. rodentium intestinal colonization. ..
Hays M, Ericsson A, Yang Y, Hardwidge P. Vaccinating with conserved Escherichia coli antigens does not alter the mouse intestinal microbiome. BMC Res Notes. 2016;9:401 pubmed publisher
..We conclude that despite the conservation of MipA, Skp, and ETEC_2479 among Gram-negative bacteria, vaccination with these antigens fails to alter significantly the host intestinal microbiome. ..
Gao X, Pham T, Feuerbacher L, Chen K, Hays M, Singh G, et al
. Citrobacter rodentium NleB Protein Inhibits Tumor Necrosis Factor (TNF) Receptor-associated Factor 3 (TRAF3) Ubiquitination to Reduce Host Type I Interferon Production. J Biol Chem. 2016;291:18232-8 pubmed publisher
..Collectively, our data reveal important interplay between GAPDH and TRAF3 and suggest a mechanism by which the NleB effector inhibits type I IFN signaling. ..
Wang G, Geisbrecht B, Rueter C, Hardwidge P. Enterotoxigenic Escherichia coli Flagellin Inhibits TNF-Induced NF-?B Activation in Intestinal Epithelial Cells. Pathogens. 2017;6: pubmed publisher
..These data suggest a potentially novel mechanism by which ETEC may evade the host innate immune response by down-regulating NF-?B-dependent host responses. ..
El Qaidi S, Chen K, Halim A, Siukstaite L, Rueter C, Hurtado Guerrero R, et al
. NleB/SseK effectors from Citrobacter rodentium, Escherichia coli, and Salmonella enterica display distinct differences in host substrate specificity. J Biol Chem. 2017;292:11423-11430 pubmed publisher
..These results provide evidence that members of this highly conserved family of bacterial virulence effectors target different host protein substrates and exhibit distinct cellular modes of action to suppress host responses. ..