Heng Zhu

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling
    Crystal L Woodard
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 8:e72671. 2013
  2. pmc Functional protein microarray as molecular decathlete: a versatile player in clinical proteomics
    Heng Zhu
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, MD, USA
    Proteomics Clin Appl 6:548-62. 2012
  3. pmc TiGER: a database for tissue-specific gene expression and regulation
    Xiong Liu
    Wilmer Institute, Johns Hopkins University School of Medicine, Maumenee Building 844, 600 N, Wolfe Street, Baltimore, MD 21287, USA
    BMC Bioinformatics 9:271. 2008
  4. pmc PhosphoNetworks: a database for human phosphorylation networks
    Jianfei Hu
    Department of Ophthalmology, Johns Hopkins School of Medicine, Department of Pharmacology and Molecular Sciences, Center for High Throughput Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA, Department of Biology, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA and The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Bioinformatics 30:141-2. 2014
  5. doi request reprint Interactome mapping: using protein microarray technology to reconstruct diverse protein networks
    Ijeoma Uzoma
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Genomics Proteomics Bioinformatics 11:18-28. 2013
  6. doi request reprint Characterization of protein-DNA interactions using protein microarrays
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cold Spring Harb Protoc 2011:pdb.prot5614. 2011
  7. pmc Construction of human activity-based phosphorylation networks
    Robert H Newman
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Syst Biol 9:655. 2013
  8. doi request reprint Systematic characterization of protein-DNA interactions
    Zhi Xie
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Mol Life Sci 68:1657-68. 2011
  9. pmc Functional protein microarray technology
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Wiley Interdiscip Rev Syst Biol Med 3:255-68. 2011
  10. pmc Applications of functional protein microarrays in basic and clinical research
    Heng Zhu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Adv Genet 79:123-55. 2012

Collaborators

Detail Information

Publications17

  1. pmc Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling
    Crystal L Woodard
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 8:e72671. 2013
    ....
  2. pmc Functional protein microarray as molecular decathlete: a versatile player in clinical proteomics
    Heng Zhu
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, MD, USA
    Proteomics Clin Appl 6:548-62. 2012
    ..In this review, we will summarize some of the most innovative and exciting recent applications of protein microarrays in clinical proteomics, including biomarker identification, pathogen-host interactions, and cancer biology...
  3. pmc TiGER: a database for tissue-specific gene expression and regulation
    Xiong Liu
    Wilmer Institute, Johns Hopkins University School of Medicine, Maumenee Building 844, 600 N, Wolfe Street, Baltimore, MD 21287, USA
    BMC Bioinformatics 9:271. 2008
    ..Understanding how genes are expressed and regulated in different tissues is a fundamental and challenging question. However, most of currently available biological databases do not focus on tissue-specific gene regulation...
  4. pmc PhosphoNetworks: a database for human phosphorylation networks
    Jianfei Hu
    Department of Ophthalmology, Johns Hopkins School of Medicine, Department of Pharmacology and Molecular Sciences, Center for High Throughput Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA, Department of Biology, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA and The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Bioinformatics 30:141-2. 2014
    ..In addition, the database also includes several analytical tools for dissecting phosphorylation networks. PhosphoNetworks is expected to play a prominent role in proteomics and phosphorylation-related disease research...
  5. doi request reprint Interactome mapping: using protein microarray technology to reconstruct diverse protein networks
    Ijeoma Uzoma
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Genomics Proteomics Bioinformatics 11:18-28. 2013
    ..We will also discuss emerging applications and future directions of protein microarray technology in the global frontier...
  6. doi request reprint Characterization of protein-DNA interactions using protein microarrays
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cold Spring Harb Protoc 2011:pdb.prot5614. 2011
    ..This approach provides simultaneous identification of PDIs for thousands of proteins, and multiple carefully designed DNA probes can be tested in parallel, which enables a rapid mapping of PDIs on a proteome-wide scale...
  7. pmc Construction of human activity-based phosphorylation networks
    Robert H Newman
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Syst Biol 9:655. 2013
    ..Overall, these studies provide global insights into kinase-mediated signaling pathways and promise to advance our understanding of cellular signaling processes in humans...
  8. doi request reprint Systematic characterization of protein-DNA interactions
    Zhi Xie
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Mol Life Sci 68:1657-68. 2011
    ..The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks...
  9. pmc Functional protein microarray technology
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Wiley Interdiscip Rev Syst Biol Med 3:255-68. 2011
    ..We strongly believe that functional protein microarrays will soon become an indispensible and invaluable tool in proteomics research and systems biology...
  10. pmc Applications of functional protein microarrays in basic and clinical research
    Heng Zhu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Adv Genet 79:123-55. 2012
    ..As a powerful technology platform, it would not be surprising if protein microarrays will become one of the leading technologies in proteomic and diagnostic fields in the next decade...
  11. pmc Integrative analysis of tissue-specific methylation and alternative splicing identifies conserved transcription factor binding motifs
    Jun Wan
    Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, 21287 Baltimore, MD, USA, Department of Pharmacology and Molecular Science, Johns Hopkins University School of Medicine, 600 North Wolfe Street, 21287 Baltimore, MD, USA, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 600 North Wolfe Street, 21287 Baltimore, MD, USA, Department of Neuroscience, Johns Hopkins University School of Medicine, 600 North Wolfe Street, 21287 Baltimore, MD, USA, Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, 21287 Baltimore, MD, USA and Institut de la Vision, 17 rue Moreau, 75012 Paris, France
    Nucleic Acids Res 41:8503-14. 2013
    ..Our results suggest that DNA methylation-dependent alternative splicing is widespread and lay the foundation for further mechanistic studies of the role of DNA methylation in tissue-specific splicing regulation. ..
  12. pmc VirD: a virion display array for profiling functional membrane proteins
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Anal Chem 85:8046-54. 2013
    ..This method can be readily scaled or tailored for different modalities including a high-content, high-throughput platform for screening ligands and drugs of human membrane proteins. ..
  13. pmc Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis
    Mary Katherine Tarrant
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Chem Biol 8:262-9. 2012
    ..This study suggests how a promiscuous protein kinase can be regulated at multiple levels to achieve particular biological outputs...
  14. pmc Global tumor protein p53/p63 interactome: making a case for cisplatin chemoresistance
    Yiping Huang
    Department of Dermatology, Institute of Basic Biomedical Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Cycle 11:2367-79. 2012
    ..Overall, our studies provide an integrated proteomic platform in making a case for the role of the p53/p63 interactome in cisplatin chemoresistance...
  15. doi request reprint A functional protein microarray approach to characterizing posttranslational modifications on lysine residues
    Jun Seop Jeong
    Department of Pharmacology and Molecular Sciences, High Throughput Biology Center, Johns Hopkins School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 723:213-23. 2011
    ..In this chapter, we will provide detailed protocols for the investigation of ubiquitylation and acetylation. These protocols are generally applicable for different organisms...
  16. pmc Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers
    Sheng Ce Tao
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University, Baltimore, MD 21205, USA
    Glycobiology 18:761-9. 2008
    ..Thus, lectin microarrays are an effective tool for analyzing diverse cell processes including cell development and differentiation, cell-cell communication, pathogen-host recognition, and cell surface biomarker identification...
  17. pmc Protein array identification of substrates of the Epstein-Barr virus protein kinase BGLF4
    Jian Zhu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, BRB 333, 733 N Broadway Ave, Baltimore, MD 21205, USA
    J Virol 83:5219-31. 2009
    ..We propose that BGLF4 contributes to effective EBV lytic cycle progression, not only through phosphorylation of EBV lytic DNA replication and virion proteins, but also by interfering with the EBNA1 replication function...