Nicholas C Zachos

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Molecular physiology of intestinal Na+/H+ exchange
    Nicholas C Zachos
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Annu Rev Physiol 67:411-43. 2005
  2. doi request reprint PLC-γ directly binds activated c-Src, which is necessary for carbachol-mediated inhibition of NHE3 activity in Caco-2/BBe cells
    Nicholas C Zachos
    Department of Medicine Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Physiol Cell Physiol 305:C266-75. 2013
  3. pmc NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Am J Physiol Cell Physiol 301:C126-36. 2011
  4. pmc Elevated intracellular calcium stimulates NHE3 activity by an IKEPP (NHERF4) dependent mechanism
    Nicholas C Zachos
    Department of Medicine and Physiology, Hopkins Center for Epithelial Disorders, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2195, USA
    Cell Physiol Biochem 22:693-704. 2008
  5. pmc Calmodulin kinase II constitutively binds, phosphorylates, and inhibits brush border Na+/H+ exchanger 3 (NHE3) by a NHERF2 protein-dependent process
    Mirza Zizak
    Division of Gastroenterology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 287:13442-56. 2012
  6. pmc Elevated calcium acutely regulates dynamic interactions of NHERF2 and NHE3 proteins in opossum kidney (OK) cell microvilli
    Xinjun Zhu
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 286:34486-96. 2011
  7. pmc NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity
    Rafiquel Sarker
    Gastroenterology and Hepatology Division, Department of Medicine, Johns Hopkins Univ School of Medicine, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 300:C771-82. 2011
  8. pmc NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells
    Jianbo Yang
    Department of Medicine, Division of Gastroenterology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 288:16960-74. 2013
  9. pmc Phospholipase C-gamma binds directly to the Na+/H+ exchanger 3 and is required for calcium regulation of exchange activity
    Nicholas C Zachos
    Division of Gastroenterology and Hepatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:19437-44. 2009
  10. doi request reprint Clcn5 knockout mice exhibit novel immunomodulatory effects and are more susceptible to dextran sulfate sodium-induced colitis
    Philip Alex
    Division of Gastroenterology, Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    J Immunol 184:3988-96. 2010

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Molecular physiology of intestinal Na+/H+ exchange
    Nicholas C Zachos
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Annu Rev Physiol 67:411-43. 2005
    ....
  2. doi request reprint PLC-γ directly binds activated c-Src, which is necessary for carbachol-mediated inhibition of NHE3 activity in Caco-2/BBe cells
    Nicholas C Zachos
    Department of Medicine Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Physiol Cell Physiol 305:C266-75. 2013
    ..Under elevated [Ca(2+)]i conditions, PLC-γ scaffolds c-Src into NHE3-containing multiprotein complexes before dissociation of PLC-γ from NHE3 and subsequent endocytosis of NHE3. ..
  3. pmc NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Am J Physiol Cell Physiol 301:C126-36. 2011
    ....
  4. pmc Elevated intracellular calcium stimulates NHE3 activity by an IKEPP (NHERF4) dependent mechanism
    Nicholas C Zachos
    Department of Medicine and Physiology, Hopkins Center for Epithelial Disorders, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2195, USA
    Cell Physiol Biochem 22:693-704. 2008
    ..This demonstrates that regulation of NHE3 depends on the nature of the NHERF family member associating with NHE3 and the accompanying NHE3 complexes...
  5. pmc Calmodulin kinase II constitutively binds, phosphorylates, and inhibits brush border Na+/H+ exchanger 3 (NHE3) by a NHERF2 protein-dependent process
    Mirza Zizak
    Division of Gastroenterology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 287:13442-56. 2012
    ..CaMKII binding to and phosphorylation of the NHE3 C terminus are parts of the physiologic regulation of NHE3 that occurs in fibroblasts as well as in the BB of an intestinal Na(+)-absorptive cell...
  6. pmc Elevated calcium acutely regulates dynamic interactions of NHERF2 and NHE3 proteins in opossum kidney (OK) cell microvilli
    Xinjun Zhu
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 286:34486-96. 2011
    ..3) The change in NHE3-NHERF2 association is accompanied by an increased BB mobile fraction of NHE3, which contributes to inhibition of NHE3 transport activity via increased endocytosis...
  7. pmc NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity
    Rafiquel Sarker
    Gastroenterology and Hepatology Division, Department of Medicine, Johns Hopkins Univ School of Medicine, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 300:C771-82. 2011
    ..cAMP-dependent inhibition of NHE3 activity requires either NHERF1 or NHERF2. Stimulation of NHE3 activity by EGF is NHERF1 dependent...
  8. pmc NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells
    Jianbo Yang
    Department of Medicine, Division of Gastroenterology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 288:16960-74. 2013
    ..Thus, multiple functions of NHERF2 require involvement of an additional domain in this protein...
  9. pmc Phospholipase C-gamma binds directly to the Na+/H+ exchanger 3 and is required for calcium regulation of exchange activity
    Nicholas C Zachos
    Division of Gastroenterology and Hepatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:19437-44. 2009
    ..These results mirror previous studies with the transient receptor potential channel and suggest that PLC-gamma may play a common role in regulating the cell-surface expression of ion transporters...
  10. doi request reprint Clcn5 knockout mice exhibit novel immunomodulatory effects and are more susceptible to dextran sulfate sodium-induced colitis
    Philip Alex
    Division of Gastroenterology, Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    J Immunol 184:3988-96. 2010
    ....
  11. pmc NHE3 mobility in brush borders increases upon NHERF2-dependent stimulation by lyophosphatidic acid
    Boyoung Cha
    Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 212052, USA
    J Cell Sci 123:2434-43. 2010
    ....
  12. pmc Regulation of intestinal electroneutral sodium absorption and the brush border Na+/H+ exchanger by intracellular calcium
    Nicholas C Zachos
    Department of Medicine, Division of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Ann N Y Acad Sci 1165:240-8. 2009
    ..We will also present new data on using advanced imaging showing rapid BB NHE3 endocytosis in response to elevated [Ca(2+)](i)...
  13. pmc NHERF3 (PDZK1) contributes to basal and calcium inhibition of NHE3 activity in Caco-2BBe cells
    Nicholas C Zachos
    Department of Medicine, Hopkins Center for Epithelial Disorders, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    J Biol Chem 284:23708-18. 2009
    ..These results demonstrate that NHERF3 reconstitutes calcium inhibition of NHE3 activity by anchoring NHE3 basally and releasing it with elevated Ca(2+)...
  14. pmc NHE3 activity is dependent on direct phosphoinositide binding at the N terminus of its intracellular cytosolic region
    Sachin Mohan
    Department of Physiology and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 285:34566-78. 2010
    ....
  15. pmc NHE3 regulatory complexes
    Mark Donowitz
    Johns Hopkins University School of Medicine, 720 Rutland Avenue Baltimore, MD 21205, USA
    J Exp Biol 212:1638-46. 2009
    ..This is a review of the domain structure of NHE3 and of the scaffolding function and role in the regulation of NHE3 of the NHE3 C-terminal domain...
  16. pmc Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis
    Philip Alex
    Div of Gastroenterology, Department of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Inflamm Bowel Dis 15:341-52. 2009
    ....
  17. pmc Epac1 mediates protein kinase A-independent mechanism of forskolin-activated intestinal chloride secretion
    Kazi Mirajul Hoque
    Department of Medicine, GI Division, Johns Hopkins University, Baltimore, MD 21205, USA
    J Gen Physiol 135:43-58. 2010
    ..These results led us to conclude that the Epac-Rap-PLC-[Ca2+]i signaling pathway is involved in cAMP-stimulated Cl- secretion, which is carried by a novel, previously undescribed Cl- channel...
  18. pmc Translating molecular physiology of intestinal transport into pharmacologic treatment of diarrhea: stimulation of Na+ absorption
    Varsha Singh
    Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Clin Gastroenterol Hepatol 12:27-31. 2014
    ..Mechanisms of Cl(-) secretion and approaches to anti-Cl(-) secretory therapies of diarrhea are discussed in a companion review. ..
  19. ncbi request reprint Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum versus
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 282:25141-51. 2007
    ....
  20. pmc Downregulation of sodium transporters and NHERF proteins in IBD patients and mouse colitis models: potential contributors to IBD-associated diarrhea
    Sean Sullivan
    GI Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Inflamm Bowel Dis 15:261-74. 2009
    ..This study was designed to test the hypothesis that intestinal Na(+)-related transporters/channels and their regulatory proteins may be downregulated as a potential contributor to IBD-associated diarrhea...
  21. pmc NHERF family and NHE3 regulation
    Mark Donowitz
    John Hopkins University School of Medicine, Departments of Medicine and Physiology, Baltimore, MD 21205, USA
    J Physiol 567:3-11. 2005
    ..In this case, NHERF2 directly binds cGKII in the brush border to form an NHE3 complex, with cGKII also associating with the BB via its myristoylation...
  22. pmc Carbachol-mediated endocytosis of NHE3 involves a clathrin-independent mechanism requiring lipid rafts and Cdc42
    Nicholas C Zachos
    Department of Medicine Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Physiol Biochem 33:869-81. 2014
    ....
  23. ncbi request reprint Proteome of murine jejunal brush border membrane vesicles
    Mark Donowitz
    J Proteome Res 6:4068-79. 2007
    ..This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine...
  24. ncbi request reprint Regulation of oocyte microvilli development in the baboon fetal ovary by estrogen
    Nicholas C Zachos
    Department of Physiological Sciences, Eastern Virginia Medical School, P O Box 1980, Norfolk, VA 23501 1980, USA
    Endocrinology 145:959-66. 2004
    ....