Nicholas C Zachos

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi Molecular physiology of intestinal Na+/H+ exchange
    Nicholas C Zachos
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Annu Rev Physiol 67:411-43. 2005
  2. ncbi NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Am J Physiol Cell Physiol 301:C126-36. 2011
  3. ncbi Elevated intracellular calcium stimulates NHE3 activity by an IKEPP (NHERF4) dependent mechanism
    Nicholas C Zachos
    Department of Medicine and Physiology, Hopkins Center for Epithelial Disorders, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2195, USA
    Cell Physiol Biochem 22:693-704. 2008
  4. ncbi Calmodulin kinase II constitutively binds, phosphorylates, and inhibits brush border Na+/H+ exchanger 3 (NHE3) by a NHERF2 protein-dependent process
    Mirza Zizak
    Division of Gastroenterology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 287:13442-56. 2012
  5. ncbi Elevated calcium acutely regulates dynamic interactions of NHERF2 and NHE3 proteins in opossum kidney (OK) cell microvilli
    Xinjun Zhu
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 286:34486-96. 2011
  6. ncbi NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity
    Rafiquel Sarker
    Gastroenterology and Hepatology Division, Department of Medicine, Johns Hopkins Univ School of Medicine, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 300:C771-82. 2011
  7. ncbi Phospholipase C-gamma binds directly to the Na+/H+ exchanger 3 and is required for calcium regulation of exchange activity
    Nicholas C Zachos
    Division of Gastroenterology and Hepatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:19437-44. 2009
  8. ncbi Clcn5 knockout mice exhibit novel immunomodulatory effects and are more susceptible to dextran sulfate sodium-induced colitis
    Philip Alex
    Division of Gastroenterology, Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    J Immunol 184:3988-96. 2010
  9. ncbi NHE3 mobility in brush borders increases upon NHERF2-dependent stimulation by lyophosphatidic acid
    Boyoung Cha
    Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 212052, USA
    J Cell Sci 123:2434-43. 2010
  10. ncbi Regulation of intestinal electroneutral sodium absorption and the brush border Na+/H+ exchanger by intracellular calcium
    Nicholas C Zachos
    Department of Medicine, Division of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Ann N Y Acad Sci 1165:240-8. 2009

Collaborators

Detail Information

Publications20

  1. ncbi Molecular physiology of intestinal Na+/H+ exchange
    Nicholas C Zachos
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Annu Rev Physiol 67:411-43. 2005
    ....
  2. ncbi NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Am J Physiol Cell Physiol 301:C126-36. 2011
    ....
  3. ncbi Elevated intracellular calcium stimulates NHE3 activity by an IKEPP (NHERF4) dependent mechanism
    Nicholas C Zachos
    Department of Medicine and Physiology, Hopkins Center for Epithelial Disorders, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2195, USA
    Cell Physiol Biochem 22:693-704. 2008
    ..This demonstrates that regulation of NHE3 depends on the nature of the NHERF family member associating with NHE3 and the accompanying NHE3 complexes...
  4. ncbi Calmodulin kinase II constitutively binds, phosphorylates, and inhibits brush border Na+/H+ exchanger 3 (NHE3) by a NHERF2 protein-dependent process
    Mirza Zizak
    Division of Gastroenterology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 287:13442-56. 2012
    ..CaMKII binding to and phosphorylation of the NHE3 C terminus are parts of the physiologic regulation of NHE3 that occurs in fibroblasts as well as in the BB of an intestinal Na(+)-absorptive cell...
  5. ncbi Elevated calcium acutely regulates dynamic interactions of NHERF2 and NHE3 proteins in opossum kidney (OK) cell microvilli
    Xinjun Zhu
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 286:34486-96. 2011
    ..3) The change in NHE3-NHERF2 association is accompanied by an increased BB mobile fraction of NHE3, which contributes to inhibition of NHE3 transport activity via increased endocytosis...
  6. ncbi NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity
    Rafiquel Sarker
    Gastroenterology and Hepatology Division, Department of Medicine, Johns Hopkins Univ School of Medicine, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 300:C771-82. 2011
    ..cAMP-dependent inhibition of NHE3 activity requires either NHERF1 or NHERF2. Stimulation of NHE3 activity by EGF is NHERF1 dependent...
  7. ncbi Phospholipase C-gamma binds directly to the Na+/H+ exchanger 3 and is required for calcium regulation of exchange activity
    Nicholas C Zachos
    Division of Gastroenterology and Hepatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:19437-44. 2009
    ..These results mirror previous studies with the transient receptor potential channel and suggest that PLC-gamma may play a common role in regulating the cell-surface expression of ion transporters...
  8. ncbi Clcn5 knockout mice exhibit novel immunomodulatory effects and are more susceptible to dextran sulfate sodium-induced colitis
    Philip Alex
    Division of Gastroenterology, Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    J Immunol 184:3988-96. 2010
    ....
  9. ncbi NHE3 mobility in brush borders increases upon NHERF2-dependent stimulation by lyophosphatidic acid
    Boyoung Cha
    Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 212052, USA
    J Cell Sci 123:2434-43. 2010
    ....
  10. ncbi Regulation of intestinal electroneutral sodium absorption and the brush border Na+/H+ exchanger by intracellular calcium
    Nicholas C Zachos
    Department of Medicine, Division of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Ann N Y Acad Sci 1165:240-8. 2009
    ..We will also present new data on using advanced imaging showing rapid BB NHE3 endocytosis in response to elevated [Ca(2+)](i)...
  11. ncbi NHE3 activity is dependent on direct phosphoinositide binding at the N terminus of its intracellular cytosolic region
    Sachin Mohan
    Department of Physiology and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 285:34566-78. 2010
    ....
  12. ncbi Epac1 mediates protein kinase A-independent mechanism of forskolin-activated intestinal chloride secretion
    Kazi Mirajul Hoque
    Department of Medicine, GI Division, Johns Hopkins University, Baltimore, MD 21205, USA
    J Gen Physiol 135:43-58. 2010
    ..These results led us to conclude that the Epac-Rap-PLC-[Ca2+]i signaling pathway is involved in cAMP-stimulated Cl- secretion, which is carried by a novel, previously undescribed Cl- channel...
  13. ncbi NHERF3 (PDZK1) contributes to basal and calcium inhibition of NHE3 activity in Caco-2BBe cells
    Nicholas C Zachos
    Department of Medicine, Hopkins Center for Epithelial Disorders, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    J Biol Chem 284:23708-18. 2009
    ..These results demonstrate that NHERF3 reconstitutes calcium inhibition of NHE3 activity by anchoring NHE3 basally and releasing it with elevated Ca(2+)...
  14. ncbi NHE3 regulatory complexes
    Mark Donowitz
    Johns Hopkins University School of Medicine, 720 Rutland Avenue Baltimore, MD 21205, USA
    J Exp Biol 212:1638-46. 2009
    ..This is a review of the domain structure of NHE3 and of the scaffolding function and role in the regulation of NHE3 of the NHE3 C-terminal domain...
  15. ncbi Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis
    Philip Alex
    Div of Gastroenterology, Department of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Inflamm Bowel Dis 15:341-52. 2009
    ....
  16. ncbi Downregulation of sodium transporters and NHERF proteins in IBD patients and mouse colitis models: potential contributors to IBD-associated diarrhea
    Sean Sullivan
    GI Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Inflamm Bowel Dis 15:261-74. 2009
    ..This study was designed to test the hypothesis that intestinal Na(+)-related transporters/channels and their regulatory proteins may be downregulated as a potential contributor to IBD-associated diarrhea...
  17. ncbi Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum versus
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 282:25141-51. 2007
    ....
  18. ncbi NHERF family and NHE3 regulation
    Mark Donowitz
    John Hopkins University School of Medicine, Departments of Medicine and Physiology, Baltimore, MD 21205, USA
    J Physiol 567:3-11. 2005
    ..In this case, NHERF2 directly binds cGKII in the brush border to form an NHE3 complex, with cGKII also associating with the BB via its myristoylation...
  19. ncbi Proteome of murine jejunal brush border membrane vesicles
    Mark Donowitz
    J Proteome Res 6:4068-79. 2007
    ..This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine...
  20. ncbi Regulation of oocyte microvilli development in the baboon fetal ovary by estrogen
    Nicholas C Zachos
    Department of Physiological Sciences, Eastern Virginia Medical School, P.O. Box 1980, Norfolk, VA 23501-1980, USA
    Endocrinology 145:959-66. 2004
    ....