A A Zachary

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint 14th International HLA and Immunogenetics Workshop: report on understanding antibodies in transplantation
    A A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
    Tissue Antigens 69:160-73. 2007
  2. doi request reprint HLA antibody detection and characterization by solid phase immunoassays: methods and pitfalls
    Andrea A Zachary
    Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 882:289-308. 2012
  3. doi request reprint Rituximab prevents an anamnestic response in patients with cryptic sensitization to HLA
    Andrea A Zachary
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Transplantation 95:701-4. 2013
  4. ncbi request reprint Specific and durable elimination of antibody to donor HLA antigens in renal-transplant patients
    Andrea A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
    Transplantation 76:1519-25. 2003
  5. doi request reprint Naturally occurring interference in Luminex assays for HLA-specific antibodies: characteristics and resolution
    Andrea A Zachary
    Departments of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Hum Immunol 70:496-501. 2009
  6. doi request reprint Immunogenetics and immunology in transplantation
    Andrea A Zachary
    Immunogenetics Laboratory, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Immunol Res 47:232-9. 2010
  7. doi request reprint Antibody monitoring: a solid approach to predicting clinical outcome
    Andrea A Zachary
    Immunogenetics Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Transplantation 86:768. 2008
  8. doi request reprint Detecting and monitoring human leukocyte antigen-specific antibodies
    Andrea A Zachary
    Immunogenetics Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Hum Immunol 69:591-604. 2008
  9. ncbi request reprint HLA-specific B cells: I. A method for their detection, quantification, and isolation using HLA tetramers
    Andrea A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Transplantation 83:982-8. 2007
  10. ncbi request reprint Factors associated with and predictive of persistence of donor-specific antibody after treatment with plasmapheresis and intravenous immunoglobulin
    Andrea A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
    Hum Immunol 66:364-70. 2005

Detail Information

Publications51

  1. ncbi request reprint 14th International HLA and Immunogenetics Workshop: report on understanding antibodies in transplantation
    A A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
    Tissue Antigens 69:160-73. 2007
    ..This report is an integration of the presentations of those scientists...
  2. doi request reprint HLA antibody detection and characterization by solid phase immunoassays: methods and pitfalls
    Andrea A Zachary
    Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 882:289-308. 2012
    ..Knowledge of the characteristics of each product and thorough and accurate analysis of the results are essential to the utility of these assays...
  3. doi request reprint Rituximab prevents an anamnestic response in patients with cryptic sensitization to HLA
    Andrea A Zachary
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Transplantation 95:701-4. 2013
    ..Quantifying HLA-specific B cells provides a way to identify these patients and provide treatment to prevent an anamnestic response...
  4. ncbi request reprint Specific and durable elimination of antibody to donor HLA antigens in renal-transplant patients
    Andrea A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
    Transplantation 76:1519-25. 2003
    ..Attempts to deal with this problem have used plasmapheresis to remove antibodies or high-dose pooled immunoglobulin (IVIg) to down-regulate DSA. However, elimination of antibodies by these methods has been limited in duration or scope...
  5. doi request reprint Naturally occurring interference in Luminex assays for HLA-specific antibodies: characteristics and resolution
    Andrea A Zachary
    Departments of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Hum Immunol 70:496-501. 2009
    ..Because interference is frequently observed in sera from highly sensitized patients, it is important to recognize and eliminate interference in Luminex antibody assays for accurate and meaningful test interpretation...
  6. doi request reprint Immunogenetics and immunology in transplantation
    Andrea A Zachary
    Immunogenetics Laboratory, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Immunol Res 47:232-9. 2010
    ..Ongoing studies are investigating the mechanisms underlying the down-regulation of donor HLA-specific antibodies...
  7. doi request reprint Antibody monitoring: a solid approach to predicting clinical outcome
    Andrea A Zachary
    Immunogenetics Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Transplantation 86:768. 2008
  8. doi request reprint Detecting and monitoring human leukocyte antigen-specific antibodies
    Andrea A Zachary
    Immunogenetics Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Hum Immunol 69:591-604. 2008
    ..Here we review the various tests available and provide guidelines for the development of monitoring protocols...
  9. ncbi request reprint HLA-specific B cells: I. A method for their detection, quantification, and isolation using HLA tetramers
    Andrea A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Transplantation 83:982-8. 2007
    ....
  10. ncbi request reprint Factors associated with and predictive of persistence of donor-specific antibody after treatment with plasmapheresis and intravenous immunoglobulin
    Andrea A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
    Hum Immunol 66:364-70. 2005
    ..We demonstrate how the types of data generated here can be used to predict elimination or persistence of DSA...
  11. ncbi request reprint HLA-specific B cells: II. Application to transplantation
    Andrea A Zachary
    Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
    Transplantation 83:989-94. 2007
    ..These differences may reflect differences in immune competency and/or exposure to alloantigens. We have investigated the frequencies and phenotypes of HLA-specific B cells to address those possibilities...
  12. doi request reprint Using real data for a virtual crossmatch
    Andrea A Zachary
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Hum Immunol 70:574-9. 2009
    ..We also present data demonstrating an additional use of the virtual crossmatch in posttransplant monitoring...
  13. ncbi request reprint Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: I. Evaluation of the GTI QuikID assay and analysis of antibody patterns
    A A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Hum Immunol 62:228-35. 2001
    ..Interestingly, the frequencies of different antibodies detected by ELISA differed from those detected by CYT with ELISA identifying more sera containing antibodies to both A and B locus antigens...
  14. ncbi request reprint Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: II. Clinical relevance
    A A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Hum Immunol 62:236-46. 2001
    ..Finally, we present case studies to illustrate the value of ELISA in predicting humoral rejection episodes and in monitoring the efficacy of rejection therapies...
  15. doi request reprint Desensitization: achieving immune detente
    A A Zachary
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Tissue Antigens 77:3-8. 2011
    ..Further, in addition to increasing the rate of transplantation among sensitized patients, desensitization is providing insight into immunoregulatory processes and may provide information useful in diseases involving immune dysfunction...
  16. doi request reprint Barriers to successful transplantation of the sensitized patient
    Andrea A Zachary
    Immunogenetics Laboratory, Johns Hopkins University, School of Medicine, 2041 E Monument Street, Baltimore, MD 21205, USA
    Expert Rev Clin Immunol 6:449-60. 2010
    ..Here, we review the characteristics of antibodies believed to be relevant to transplant success and the various approaches to dealing with them...
  17. ncbi request reprint Antigen, allele, and haplotype frequencies report of the ASHI minority antigens workshops: part 1, African-Americans
    A A Zachary
    Immunogenetics Laboratory, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Hum Immunol 62:1127-36. 2001
    ..0013), lower than expected frequency of inheritance (44.5% vs 54.5%) of the DRB1*1503; DQB1*0602 haplotype, lower than anticipated linkage disequilibrium values for DR; DQ haplotypes, and a skewed geographic distribution of DR antigens...
  18. doi request reprint Eculizumab, bortezomib and kidney paired donation facilitate transplantation of a highly sensitized patient without vascular access
    B E Lonze
    Division of Transplant Surgery, Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Transplant 10:2154-60. 2010
    ....
  19. ncbi request reprint Subclinical acute antibody-mediated rejection in positive crossmatch renal allografts
    M Haas
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA
    Am J Transplant 7:576-85. 2007
    ..5 +/- 2.5 versus 1.0 +/- 2.0, p = 0.01) than that in 24 recipients of HLA-incompatible grafts with no AMR over a similar interval (360 +/- 117 days), suggesting that subclinical AMR may contribute to development of CAN...
  20. ncbi request reprint Effect of Bw4 and Bw6 epitope mismatches on antibody production, acute and chronic rejection, and graft survival in renal allografts
    M S Leffell
    Depaertment of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Transplantation 72:433-7. 2001
    ..CONCLUSIONS: Mismatching for Bw4 or Bw6 does not confer any independent, increased risk for humoral sensitization or renal allograft failure...
  21. doi request reprint Proinflammatory events are associated with significant increases in breadth and strength of HLA-specific antibody
    J E Locke
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Transplant 9:2136-9. 2009
    ..25, (95% CI 4.03-6.85), p < 0.001] versus 2.5-fold [IRR 2.54, (95% CI 1.64-3.95), p < 0.001] increase in HSA. Therefore, sensitized patients known to have an infection or undergoing surgery should be monitored for expansion of HSA...
  22. ncbi request reprint CD20-positive infiltrates in renal allograft biopsies with acute cellular rejection are not associated with worse graft survival
    S M Bagnasco
    Department of Pathology, Johns Hopkins University, Baltimore, MD, USA
    Am J Transplant 7:1968-73. 2007
    ..Our data do not support association of B cell-rich infiltrates in allograft biopsies and worse outcome in acute rejection type I or II, but do not exclude the possible contribution of B cells to allograft rejection...
  23. doi request reprint The use of antibody to complement protein C5 for salvage treatment of severe antibody-mediated rejection
    J E Locke
    Deparmtent of Surgery, John Hopkins Medical Institutions, John Hopkins University, Baltimore, MD, USA
    Am J Transplant 9:231-5. 2009
    ..We show a marked decrease in C5b-C9 (MAC) complex deposition in the kidney after the administration of eculizumab...
  24. doi request reprint Subclinical rejection in stable positive crossmatch kidney transplant patients: incidence and correlations
    E S Kraus
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Transplant 9:1826-34. 2009
    ..Surveillance biopsies during the first year post-transplantation for these high-risk patients uncover clinically occult processes and phenotypes, which without intervention diminish allograft survival and function...
  25. ncbi request reprint The utility of splenectomy as rescue treatment for severe acute antibody mediated rejection
    J E Locke
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Transplant 7:842-6. 2007
    ..Five patients underwent immediate splenectomy followed by PP/IVIg and had return of allograft function within 48 h of the procedure. Emergent splenectomy followed by PP/IVIg may be an effective treatment for reversing severe AMR...
  26. pmc Detection of HLA class I-specific antibodies by the QuikScreen enzyme-linked immunosorbent assay
    D P Lucas
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Clin Diagn Lab Immunol 4:252-7. 1997
    ..As an adjunct to serum screening by cytotoxicity testing, the GTI QuikScreen test can produce a substantial savings of time and effort that reduces the cost to the laboratory and to the patient...
  27. ncbi request reprint C4d and C3d staining in biopsies of ABO- and HLA-incompatible renal allografts: correlation with histologic findings
    M Haas
    Department of Pathology, John Hopkins Medical Institutions, Baltimore, Maryland, USA
    Am J Transplant 6:1829-40. 2006
    ....
  28. ncbi request reprint Cohort study of the prognostic significance of acute transplant glomerulitis in acutely rejecting renal allografts
    N C Messias
    Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD 21287, USA
    Transplantation 72:655-60. 2001
    ..97; P=0.97). CONCLUSION: In this study, G was significantly more common in highly sensitized patients and was strongly associated with vascular rejection biopsies but was not an independent predictor of graft survival...
  29. ncbi request reprint Preemptive therapy with plasmapheresis/intravenous immunoglobulin allows successful live donor renal transplantation in patients with a positive cross-match
    C J Sonnenday
    Department of Surgery, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Transplant Proc 34:1614-6. 2002
  30. doi request reprint Successful three-way kidney paired donation with cross-country live donor allograft transport
    R A Montgomery
    Department of Surgery, Johns Hopkins University, Baltimore, MD, USA
    Am J Transplant 8:2163-8. 2008
    ..1 mg/dl. This case provides a blueprint for solving some of the complexities that are inherent in the implementation of a national KPD program in a large country like the United States...
  31. doi request reprint Eculizumab prevents recurrent antiphospholipid antibody syndrome and enables successful renal transplantation
    B E Lonze
    Division of Transplant Surgery, Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD
    Am J Transplant 14:459-65. 2014
    ..While the appropriate duration of treatment remains to be determined, this case series suggests that complement inhibitors such as eculizumab may prove to be effective in preventing the recurrence of APS after renal transplantation. ..
  32. ncbi request reprint Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodes
    W M Baldwin
    Department of Pathology, The Johns Hopkins University, Baltimore, Maryland 21205 2196, USA
    Transplantation 68:894-900. 1999
    ..In animal models, tissue ischemia has been shown to activate complement...
  33. ncbi request reprint An unusual case of fatal pulmonary allograft rejection
    P J Mogayzel
    Eudowood Division of Pediatric Respiratory Sciences, The Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, MD 21287 2533, USA
    Pediatr Transplant 5:138-41. 2001
    ..Despite aggressive therapy the patient died within 7 months of transplant. The timely recognition and treatment of unusual forms of allograft rejection is vital in the management of pulmonary transplant patients...
  34. pmc Paternal origin of FGFR2 mutations in sporadic cases of Crouzon syndrome and Pfeiffer syndrome
    R L Glaser
    Department of Pediatrics, Center for Craniofacial Development and Disorders, McKusick Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Hum Genet 66:768-77. 2000
    ..Our results suggest that older men either have accumulated or are more susceptible to a variety of germline mutations...
  35. ncbi request reprint Beyond C4d: other complement-related diagnostic approaches to antibody-mediated rejection
    William M Baldwin
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Transplant 4:311-8. 2004
    ..Understanding the mechanisms by which complement integrates the interactions of leukocytes, platelets and parenchymal cells offers opportunities to further refine the diagnosis of rejection...
  36. ncbi request reprint HLA alleles and haplotypes among the Lakota Sioux: report of the ASHI minority workshops, part III
    Mary S Leffell
    Department of Medicine, Johns Hopkins University School of Public Health, Baltimore, MD 21205, USA
    Hum Immunol 65:78-89. 2004
    ..The two most common extended haplotypes among the Sioux, determined by maximum likelihood estimation and genotyping were: A*31012, B*3501, Cw*0404, DRB1*0407; and A*24021, B*3501, Cw*0404, DRB1*0404...
  37. ncbi request reprint Changing renal allocation: is there a long-term price for short-term gain?
    Andrea A Zachary
    Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Transplantation 74:1505-6. 2002
  38. ncbi request reprint HLA antigens, alleles and haplotypes among the Yup'ik Alaska natives: report of the ASHI Minority Workshops, Part II
    Mary S Leffell
    Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    Hum Immunol 63:614-25. 2002
    ..DRB1*0401 and *1101, which occur in high frequency among these Eskimo populations, but not in other Native Americans, were also prevalent among the Yup'ik, with respective frequencies of 0.232 and 0.107...
  39. ncbi request reprint Successful renal transplantation across simultaneous ABO incompatible and positive crossmatch barriers
    Daniel S Warren
    The Department of Surgery, The Johns Hopkins University School of Medicine, Balitmore, Maryland, USA
    Am J Transplant 4:561-8. 2004
    ..The ability to cross these two barriers simultaneously is clinically important as sensitized patients have often exhausted their blood type compatible living donors during previous transplants...
  40. ncbi request reprint Consensus opinion from the antibody working group on the diagnosis, reporting, and risk assessment for antibody-mediated rejection and desensitization protocols
    Robert A Montgomery
    Department of Surgery, Division of Transplantation, Johns Hopkins University School of Medicine, Ross 765 720 Rutland Avenue, Baltimore, MD 21205, USA
    Transplantation 78:181-5. 2004
    ..The Antibody Working Group was assembled in an attempt to provide guidelines for the standardization of nomenclature, diagnostic criteria, reporting, antibody profiling, and risk assessment...
  41. ncbi request reprint Domino paired kidney donation: a strategy to make best use of live non-directed donation
    Robert A Montgomery
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Lancet 368:419-21. 2006
  42. pmc Antibody-mediated rejection in human cardiac allografts: evaluation of immunoglobulins and complement activation products C4d and C3d as markers
    E R Rodriguez
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Transplant 5:2778-85. 2005
    ..Concomitant use of C4d and C3d is very useful to diagnose AMR, when correlated with clinical parameters of graft function. AMR in heart transplant patients can occur many months or years after transplant...
  43. ncbi request reprint Improved definition of human leukocyte antigen frequencies among minorities and applicability to estimates of transplant compatibility
    Mary S Leffell
    Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Transplantation 83:964-72. 2007
    ..Because molecular typing has been increasingly used since 1996, analysis of recent donor phenotypes should provide more accurate HLA frequencies...
  44. ncbi request reprint Clinical results from transplanting incompatible live kidney donor/recipient pairs using kidney paired donation
    Robert A Montgomery
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    JAMA 294:1655-63. 2005
    ..Ethical, administrative, and logistical barriers initially proved formidable and prevented the implementation of KPD programs in the United States...
  45. ncbi request reprint A computer match program for paired and unconventional kidney exchanges
    Inessa Kaplan
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Transplant 5:2306-8. 2005
    ..With our database of 60 patients and 83 donors, we have been able to identify 122 two-way and 1230 three-way exchanges with an average run time of 30 s...
  46. ncbi request reprint The problem of transplanting the sensitized patient: whose problem is it?
    Annette M Jackson
    Immunogenetics Laboratory, Johns Hopkins University School of Medicine, 2041 E Monument Street, Baltimore, MD 21205 2222, USA
    Front Biosci 13:1396-412. 2008
    ..Thus, sensitization to HLA antigens is a problem for the patient, the transplant physician, and the histocompatibility laboratory...
  47. pmc Donor ethnicity influences outcomes following deceased-donor kidney transplantation in black recipients
    Jayme E Locke
    Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    J Am Soc Nephrol 19:2011-9. 2008
    ..41; 95% confidence interval 0.2 to 0.87; P = 0.02) among black recipients. These findings suggest that kidneys obtained from black donors after cardiac death may afford the best long-term survival for black recipients...
  48. ncbi request reprint Transplanting patients with a positive donor-specific crossmatch: a single center's perspective
    Robert A Montgomery
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Pediatr Transplant 8:535-42. 2004
    ..In this article we have summarized our evolving experience with the assessment, treatment, transplantation, and monitoring of patients who undergo preconditioning for a (+) XM with a live donor...
  49. ncbi request reprint Antithymocyte globulin is associated with complement deposition in cardiac transplant biopsies
    William M Baldwin
    Department of Pathology, The Johns Hopkins University, Baltimore, MD 21205 2196, USA
    Hum Immunol 65:1273-80. 2004
    ..Thus prophylactic treatment with ATGAM is associated with the deposition of horse IgG and activation of complement in the transplant...
  50. ncbi request reprint HLA-DR4 in families with autism
    Li Ching Lee
    Center for Autism and Developmental Disabilities Epidemiology, Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    Pediatr Neurol 35:303-7. 2006
    ..Such immune interactions may involve HLA and related genes in both genetic and epigenetic mechanisms during pregnancy...
  51. ncbi request reprint TNF-alpha, TGF-beta, IL-10, IL-6, and INF-gamma alleles among African Americans and Cuban Americans. Report of the ASHI Minority Workshops: Part IV
    Nancy L Delaney
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Hum Immunol 65:1413-9. 2004
    ..004). Because levels of expression can affect inflammation and immune regulation, differences in cytokine allele frequencies between racial or ethnic groups may contribute to different incidences of autoimmunity and allograft rejection...