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Genomes and GenesSpecies | A A ZacharySummaryAffiliation: Johns Hopkins University Country: USA Publications
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Publications
Rituximab prevents an anamnestic response in patients with cryptic sensitization to HLAAndrea A Zachary
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Transplantation 95:701-4. 2013..Quantifying HLA-specific B cells provides a way to identify these patients and provide treatment to prevent an anamnestic response...- HLA antibody detection and characterization by solid phase immunoassays: methods and pitfallsAndrea A Zachary
Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Methods Mol Biol 882:289-308. 2012..Knowledge of the characteristics of each product and thorough and accurate analysis of the results are essential to the utility of these assays... 
Specific and durable elimination of antibody to donor HLA antigens in renal-transplant patientsAndrea A Zachary
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
Transplantation 76:1519-25. 2003..Attempts to deal with this problem have used plasmapheresis to remove antibodies or high-dose pooled immunoglobulin (IVIg) to down-regulate DSA. However, elimination of antibodies by these methods has been limited in duration or scope...- Factors associated with and predictive of persistence of donor-specific antibody after treatment with plasmapheresis and intravenous immunoglobulinAndrea A Zachary
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
Hum Immunol 66:364-70. 2005..We demonstrate how the types of data generated here can be used to predict elimination or persistence of DSA... - 14th International HLA and Immunogenetics Workshop: report on understanding antibodies in transplantationA A Zachary
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2222, USA
Tissue Antigens 69:160-73. 2007..This report is an integration of the presentations of those scientists... - HLA-specific B cells: II. Application to transplantationAndrea A Zachary
Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
Transplantation 83:989-94. 2007..These differences may reflect differences in immune competency and/or exposure to alloantigens. We have investigated the frequencies and phenotypes of HLA-specific B cells to address those possibilities... - Detecting and monitoring human leukocyte antigen-specific antibodiesAndrea A Zachary
Immunogenetics Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Hum Immunol 69:591-604. 2008..Here we review the various tests available and provide guidelines for the development of monitoring protocols... - Naturally occurring interference in Luminex assays for HLA-specific antibodies: characteristics and resolutionAndrea A Zachary
Departments of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Hum Immunol 70:496-501. 2009..Because interference is frequently observed in sera from highly sensitized patients, it is important to recognize and eliminate interference in Luminex antibody assays for accurate and meaningful test interpretation... - Using real data for a virtual crossmatchAndrea A Zachary
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Hum Immunol 70:574-9. 2009..We also present data demonstrating an additional use of the virtual crossmatch in posttransplant monitoring... - Immunogenetics and immunology in transplantationAndrea A Zachary
Immunogenetics Laboratory, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Immunol Res 47:232-9. 2010..Ongoing studies are investigating the mechanisms underlying the down-regulation of donor HLA-specific antibodies... - Antigen, allele, and haplotype frequencies report of the ASHI minority antigens workshops: part 1, African-AmericansA A Zachary
Immunogenetics Laboratory, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Hum Immunol 62:1127-36. 2001..0013), lower than expected frequency of inheritance (44.5% vs 54.5%) of the DRB1*1503; DQB1*0602 haplotype, lower than anticipated linkage disequilibrium values for DR; DQ haplotypes, and a skewed geographic distribution of DR antigens... - Desensitization: achieving immune detenteA A Zachary
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Tissue Antigens 77:3-8. 2011..Further, in addition to increasing the rate of transplantation among sensitized patients, desensitization is providing insight into immunoregulatory processes and may provide information useful in diseases involving immune dysfunction... - Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: I. Evaluation of the GTI QuikID assay and analysis of antibody patternsA A Zachary
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Hum Immunol 62:228-35. 2001..Interestingly, the frequencies of different antibodies detected by ELISA differed from those detected by CYT with ELISA identifying more sera containing antibodies to both A and B locus antigens... - Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: II. Clinical relevanceA A Zachary
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Hum Immunol 62:236-46. 2001..Finally, we present case studies to illustrate the value of ELISA in predicting humoral rejection episodes and in monitoring the efficacy of rejection therapies... - Antibody monitoring: a solid approach to predicting clinical outcomeAndrea A Zachary
Immunogenetics Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Transplantation 86:768. 2008 - Barriers to successful transplantation of the sensitized patientAndrea A Zachary
Immunogenetics Laboratory, Johns Hopkins University, School of Medicine, 2041 E Monument Street, Baltimore, MD 21205, USA
Expert Rev Clin Immunol 6:449-60. 2010..Here, we review the characteristics of antibodies believed to be relevant to transplant success and the various approaches to dealing with them... - HLA-specific B cells: I. A method for their detection, quantification, and isolation using HLA tetramersAndrea A Zachary
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Transplantation 83:982-8. 2007.... - Eculizumab, bortezomib and kidney paired donation facilitate transplantation of a highly sensitized patient without vascular accessB E Lonze
Division of Transplant Surgery, Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Am J Transplant 10:2154-60. 2010.... - Subclinical acute antibody-mediated rejection in positive crossmatch renal allograftsM Haas
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA
Am J Transplant 7:576-85. 2007..5 +/- 2.5 versus 1.0 +/- 2.0, p = 0.01) than that in 24 recipients of HLA-incompatible grafts with no AMR over a similar interval (360 +/- 117 days), suggesting that subclinical AMR may contribute to development of CAN... - Effect of Bw4 and Bw6 epitope mismatches on antibody production, acute and chronic rejection, and graft survival in renal allograftsM S Leffell
Depaertment of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Transplantation 72:433-7. 2001..CONCLUSIONS: Mismatching for Bw4 or Bw6 does not confer any independent, increased risk for humoral sensitization or renal allograft failure... - Proinflammatory events are associated with significant increases in breadth and strength of HLA-specific antibodyJ E Locke
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Am J Transplant 9:2136-9. 2009..25, (95% CI 4.03-6.85), p < 0.001] versus 2.5-fold [IRR 2.54, (95% CI 1.64-3.95), p < 0.001] increase in HSA. Therefore, sensitized patients known to have an infection or undergoing surgery should be monitored for expansion of HSA... - CD20-positive infiltrates in renal allograft biopsies with acute cellular rejection are not associated with worse graft survivalS M Bagnasco
Department of Pathology, Johns Hopkins University, Baltimore, MD, USA
Am J Transplant 7:1968-73. 2007..Our data do not support association of B cell-rich infiltrates in allograft biopsies and worse outcome in acute rejection type I or II, but do not exclude the possible contribution of B cells to allograft rejection... 
Detection of HLA class I-specific antibodies by the QuikScreen enzyme-linked immunosorbent assayD P Lucas
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Clin Diagn Lab Immunol 4:252-7. 1997..As an adjunct to serum screening by cytotoxicity testing, the GTI QuikScreen test can produce a substantial savings of time and effort that reduces the cost to the laboratory and to the patient...- The use of antibody to complement protein C5 for salvage treatment of severe antibody-mediated rejectionJ E Locke
Deparmtent of Surgery, John Hopkins Medical Institutions, John Hopkins University, Baltimore, MD, USA
Am J Transplant 9:231-5. 2009..We show a marked decrease in C5b-C9 (MAC) complex deposition in the kidney after the administration of eculizumab... - The utility of splenectomy as rescue treatment for severe acute antibody mediated rejectionJ E Locke
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Am J Transplant 7:842-6. 2007..Five patients underwent immediate splenectomy followed by PP/IVIg and had return of allograft function within 48 h of the procedure. Emergent splenectomy followed by PP/IVIg may be an effective treatment for reversing severe AMR... - Subclinical rejection in stable positive crossmatch kidney transplant patients: incidence and correlationsE S Kraus
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Am J Transplant 9:1826-34. 2009..Surveillance biopsies during the first year post-transplantation for these high-risk patients uncover clinically occult processes and phenotypes, which without intervention diminish allograft survival and function... - C4d and C3d staining in biopsies of ABO- and HLA-incompatible renal allografts: correlation with histologic findingsM Haas
Department of Pathology, John Hopkins Medical Institutions, Baltimore, Maryland, USA
Am J Transplant 6:1829-40. 2006.... - Cohort study of the prognostic significance of acute transplant glomerulitis in acutely rejecting renal allograftsN C Messias
Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD 21287, USA
Transplantation 72:655-60. 2001..97; P=0.97). CONCLUSION: In this study, G was significantly more common in highly sensitized patients and was strongly associated with vascular rejection biopsies but was not an independent predictor of graft survival... - Preemptive therapy with plasmapheresis/intravenous immunoglobulin allows successful live donor renal transplantation in patients with a positive cross-matchC J Sonnenday
Department of Surgery, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
Transplant Proc 34:1614-6. 2002 - Successful three-way kidney paired donation with cross-country live donor allograft transportR A Montgomery
Department of Surgery, Johns Hopkins University, Baltimore, MD, USA
Am J Transplant 8:2163-8. 2008..1 mg/dl. This case provides a blueprint for solving some of the complexities that are inherent in the implementation of a national KPD program in a large country like the United States... - Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodesW M Baldwin
Department of Pathology, The Johns Hopkins University, Baltimore, Maryland 21205 2196, USA
Transplantation 68:894-900. 1999..In animal models, tissue ischemia has been shown to activate complement... - An unusual case of fatal pulmonary allograft rejectionP J Mogayzel
Eudowood Division of Pediatric Respiratory Sciences, The Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, MD 21287 2533, USA
Pediatr Transplant 5:138-41. 2001..Despite aggressive therapy the patient died within 7 months of transplant. The timely recognition and treatment of unusual forms of allograft rejection is vital in the management of pulmonary transplant patients... - Paternal origin of FGFR2 mutations in sporadic cases of Crouzon syndrome and Pfeiffer syndromeR L Glaser
Department of Pediatrics, Center for Craniofacial Development and Disorders, McKusick Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Am J Hum Genet 66:768-77. 2000..Our results suggest that older men either have accumulated or are more susceptible to a variety of germline mutations... - Changing renal allocation: is there a long-term price for short-term gain?Andrea A Zachary
Johns Hopkins University School of Medicine, Baltimore, MD, USA
Transplantation 74:1505-6. 2002 - HLA antigens, alleles and haplotypes among the Yup'ik Alaska natives: report of the ASHI Minority Workshops, Part IIMary S Leffell
Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
Hum Immunol 63:614-25. 2002..DRB1*0401 and *1101, which occur in high frequency among these Eskimo populations, but not in other Native Americans, were also prevalent among the Yup'ik, with respective frequencies of 0.232 and 0.107... - HLA alleles and haplotypes among the Lakota Sioux: report of the ASHI minority workshops, part IIIMary S Leffell
Department of Medicine, Johns Hopkins University School of Public Health, Baltimore, MD 21205, USA
Hum Immunol 65:78-89. 2004..The two most common extended haplotypes among the Sioux, determined by maximum likelihood estimation and genotyping were: A*31012, B*3501, Cw*0404, DRB1*0407; and A*24021, B*3501, Cw*0404, DRB1*0404... - Donor ethnicity influences outcomes following deceased-donor kidney transplantation in black recipientsJayme E Locke
Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
J Am Soc Nephrol 19:2011-9. 2008..41; 95% confidence interval 0.2 to 0.87; P = 0.02) among black recipients. These findings suggest that kidneys obtained from black donors after cardiac death may afford the best long-term survival for black recipients... - A computer match program for paired and unconventional kidney exchangesInessa Kaplan
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Am J Transplant 5:2306-8. 2005..With our database of 60 patients and 83 donors, we have been able to identify 122 two-way and 1230 three-way exchanges with an average run time of 30 s... - Clinical results from transplanting incompatible live kidney donor/recipient pairs using kidney paired donationRobert A Montgomery
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
JAMA 294:1655-63. 2005..Ethical, administrative, and logistical barriers initially proved formidable and prevented the implementation of KPD programs in the United States... - Antibody-mediated rejection in human cardiac allografts: evaluation of immunoglobulins and complement activation products C4d and C3d as markersE R Rodriguez
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Am J Transplant 5:2778-85. 2005..Concomitant use of C4d and C3d is very useful to diagnose AMR, when correlated with clinical parameters of graft function. AMR in heart transplant patients can occur many months or years after transplant... - Domino paired kidney donation: a strategy to make best use of live non-directed donationRobert A Montgomery
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Lancet 368:419-21. 2006 - Antithymocyte globulin is associated with complement deposition in cardiac transplant biopsiesWilliam M Baldwin
Department of Pathology, The Johns Hopkins University, Baltimore, MD 21205 2196, USA
Hum Immunol 65:1273-80. 2004..Thus prophylactic treatment with ATGAM is associated with the deposition of horse IgG and activation of complement in the transplant... - HLA-DR4 in families with autismLi Ching Lee
Center for Autism and Developmental Disabilities Epidemiology, Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
Pediatr Neurol 35:303-7. 2006..Such immune interactions may involve HLA and related genes in both genetic and epigenetic mechanisms during pregnancy... - Consensus opinion from the antibody working group on the diagnosis, reporting, and risk assessment for antibody-mediated rejection and desensitization protocolsRobert A Montgomery
Department of Surgery, Division of Transplantation, Johns Hopkins University School of Medicine, Ross 765/720 Rutland Avenue, Baltimore, MD 21205, USA
Transplantation 78:181-5. 2004..The Antibody Working Group was assembled in an attempt to provide guidelines for the standardization of nomenclature, diagnostic criteria, reporting, antibody profiling, and risk assessment... - Successful renal transplantation across simultaneous ABO incompatible and positive crossmatch barriersDaniel S Warren
The Department of Surgery, The Johns Hopkins University School of Medicine, Balitmore, Maryland, USA
Am J Transplant 4:561-8. 2004..The ability to cross these two barriers simultaneously is clinically important as sensitized patients have often exhausted their blood type compatible living donors during previous transplants... - Improved definition of human leukocyte antigen frequencies among minorities and applicability to estimates of transplant compatibilityMary S Leffell
Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Transplantation 83:964-72. 2007..Because molecular typing has been increasingly used since 1996, analysis of recent donor phenotypes should provide more accurate HLA frequencies... - Beyond C4d: other complement-related diagnostic approaches to antibody-mediated rejectionWilliam M Baldwin
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Am J Transplant 4:311-8. 2004..Understanding the mechanisms by which complement integrates the interactions of leukocytes, platelets and parenchymal cells offers opportunities to further refine the diagnosis of rejection... - The problem of transplanting the sensitized patient: whose problem is it?Annette M Jackson
Immunogenetics Laboratory, Johns Hopkins University School of Medicine, 2041 E Monument Street, Baltimore, MD 21205 2222, USA
Front Biosci 13:1396-412. 2008..Thus, sensitization to HLA antigens is a problem for the patient, the transplant physician, and the histocompatibility laboratory... - Transplanting patients with a positive donor-specific crossmatch: a single center's perspectiveRobert A Montgomery
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Pediatr Transplant 8:535-42. 2004..In this article we have summarized our evolving experience with the assessment, treatment, transplantation, and monitoring of patients who undergo preconditioning for a (+) XM with a live donor... - TNF-alpha, TGF-beta, IL-10, IL-6, and INF-gamma alleles among African Americans and Cuban Americans. Report of the ASHI Minority Workshops: Part IVNancy L Delaney
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Hum Immunol 65:1413-9. 2004..004). Because levels of expression can affect inflammation and immune regulation, differences in cytokine allele frequencies between racial or ethnic groups may contribute to different incidences of autoimmunity and allograft rejection...