Natasha E Zachara

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc The roles of O-linked β-N-acetylglucosamine in cardiovascular physiology and disease
    Natasha E Zachara
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Am J Physiol Heart Circ Physiol 302:H1905-18. 2012
  2. doi request reprint Detecting the "O-GlcNAc-ome"; detection, purification, and analysis of O-GlcNAc modified proteins
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Methods Mol Biol 534:251-79. 2009
  3. pmc The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA damage/repair and other cellular pathways
    Natasha E Zachara
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Amino Acids 40:793-808. 2011
  4. pmc Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Curr Protoc Protein Sci . 2011
  5. ncbi request reprint Perturbations in O-linked beta-N-acetylglucosamine protein modification cause severe defects in mitotic progression and cytokinesis
    Chad Slawson
    Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 280:32944-56. 2005
  6. ncbi request reprint O-GlcNAc a sensor of cellular state: the role of nucleocytoplasmic glycosylation in modulating cellular function in response to nutrition and stress
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Biochim Biophys Acta 1673:13-28. 2004
  7. doi request reprint Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University Medical School, Baltimore, Maryland, USA
    Curr Protoc Mol Biol . 2002
  8. pmc Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Curr Protoc Mol Biol . 2011
  9. pmc O-linked beta-N-acetylglucosamine (O-GlcNAc) regulates stress-induced heat shock protein expression in a GSK-3beta-dependent manner
    Zahra Kazemi
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 285:39096-107. 2010
  10. ncbi request reprint The emerging significance of O-GlcNAc in cellular regulation
    Natasha E Zachara
    The Department of Biological Chemistry, The Johns Hopkins University Medical School, Baltimore, Maryland 21205 2185, USA
    Chem Rev 102:431-8. 2002

Collaborators

  • Gerald Warren Hart
  • Akhilesh Pandey
  • Yuan Gao
  • Russell A Reeves
  • Albert Lee
  • Jennifer A Groves
  • Zahra Kazemi
  • Chad Slawson
  • Roger Henry
  • Gokben Yildirir
  • Cathrine McKen
  • Sarah Haserodt
  • Hana Chang
  • M Daniel Lane
  • Keith Vosseller
  • Win D Cheung

Detail Information

Publications15

  1. pmc The roles of O-linked β-N-acetylglucosamine in cardiovascular physiology and disease
    Natasha E Zachara
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Am J Physiol Heart Circ Physiol 302:H1905-18. 2012
    ..Here we summarize the current understanding of the roles of O-GlcNAc in the heart and vasculature...
  2. doi request reprint Detecting the "O-GlcNAc-ome"; detection, purification, and analysis of O-GlcNAc modified proteins
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Methods Mol Biol 534:251-79. 2009
    ..In this chapter we describe the most robust techniques for the detection and purification of O-GlcNAc modified proteins, and discuss some more specialized techniques for site-mapping and detection of O-GlcNAc during mass spectrometry...
  3. pmc The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA damage/repair and other cellular pathways
    Natasha E Zachara
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Amino Acids 40:793-808. 2011
    ..Supporting this hypothesis, we have shown that DNA-PK is O-GlcNAc modified in response to numerous forms of cellular stress...
  4. pmc Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Curr Protoc Protein Sci . 2011
    ..This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites...
  5. ncbi request reprint Perturbations in O-linked beta-N-acetylglucosamine protein modification cause severe defects in mitotic progression and cytokinesis
    Chad Slawson
    Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 280:32944-56. 2005
    ..These data suggest that dynamic O-GlcNAc processing is a pivotal regulatory component of the cell cycle, controlling cell cycle progression by regulating mitotic phosphorylation, cyclin expression, and cell division...
  6. ncbi request reprint O-GlcNAc a sensor of cellular state: the role of nucleocytoplasmic glycosylation in modulating cellular function in response to nutrition and stress
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Biochim Biophys Acta 1673:13-28. 2004
    ..Recently, O-GlcNAc has been implicated in the etiology of type II diabetes, the regulation of stress response pathways, and in the regulation of the proteasome...
  7. doi request reprint Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University Medical School, Baltimore, Maryland, USA
    Curr Protoc Mol Biol . 2002
    ..This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group...
  8. pmc Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Curr Protoc Mol Biol . 2011
    ..This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites...
  9. pmc O-linked beta-N-acetylglucosamine (O-GlcNAc) regulates stress-induced heat shock protein expression in a GSK-3beta-dependent manner
    Zahra Kazemi
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 285:39096-107. 2010
    ..Together, these data suggest that stress-induced GlcNAcylation increases HSP expression through inhibition of GSK-3β...
  10. ncbi request reprint The emerging significance of O-GlcNAc in cellular regulation
    Natasha E Zachara
    The Department of Biological Chemistry, The Johns Hopkins University Medical School, Baltimore, Maryland 21205 2185, USA
    Chem Rev 102:431-8. 2002
  11. pmc Dynamic O-GlcNAcylation and its roles in the cellular stress response and homeostasis
    Jennifer A Groves
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
    Cell Stress Chaperones 18:535-58. 2013
    ..Finally, we will highlight recent advancements in the tools and technologies used to study the O-GlcNAc modification...
  12. doi request reprint Detection and analysis of (O-linked beta-N-acetylglucosamine)-modified proteins
    Natasha E Zachara
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 464:227-54. 2009
    ..In this chapter we focus on methods for the detection of O-GlcNAc-modified proteins and discuss general techniques for the detection and subsequent analysis of other protein-carbohydrate conjugates...
  13. ncbi request reprint Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cells
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 279:30133-42. 2004
    ..O-GlcNAc regulates both the rates and extent of the stress-induced induction of heat shock proteins, providing a molecular basis for these findings...
  14. ncbi request reprint O-GlcNAc modification: a nutritional sensor that modulates proteasome function
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University Medical School, Baltimore, MD 21205 2185, USA
    Trends Cell Biol 14:218-21. 2004
    ..Moreover, increased glycosylation of the 19S (or PA700) regulatory subcomplex has been correlated with decreased proteasomal activity, suggesting a new model of proteasomal regulation...
  15. ncbi request reprint Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress
    Russell A Reeves
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Anal Biochem 457:8-18. 2014
    ....