Zhaohui Ye

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. doi request reprint Promise and challenges of human iPSC-based hematologic disease modeling and treatment
    Zhaohui Ye
    Division of Hematology in Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Research Building, Room 1032, Baltimore, MD 21205, USA
    Int J Hematol 95:601-9. 2012
  2. pmc Hematopoietic cells as sources for patient-specific iPSCs and disease modeling
    Zhaohui Ye
    Division of Hematology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Cycle 10:2840-4. 2011
  3. ncbi request reprint Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells
    Xiaobing Yu
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Department of Oncology, Baltimore, Maryland 21231, USA
    Mol Ther 7:827-38. 2003
  4. pmc Generation of glycosylphosphatidylinositol anchor protein-deficient blood cells from human induced pluripotent stem cells
    Xuan Yuan
    Division of Hematology, Department of Medicine, School of Medicine, and
    Stem Cells Transl Med 2:819-29. 2013
  5. pmc Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins
    Guibin Chen
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:345-55. 2008
  6. ncbi request reprint Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, Maryland 21205, USA
    Stem Cells 26:1998-2005. 2008
  7. ncbi request reprint FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cells
    Li Li
    Department of Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA
    Br J Haematol 137:64-75. 2007
  8. pmc Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders
    Zhaohui Ye
    Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Blood 114:5473-80. 2009
  9. ncbi request reprint Lentivirus-mediated gene transfer and expression in established human tumor antigen-specific cytotoxic T cells and primary unstimulated T cells
    Xianzheng Zhou
    Division of Immunology and Hematppoiesis, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Hum Gene Ther 14:1089-105. 2003
  10. pmc Notch signaling activation in human embryonic stem cells is required for embryonic, but not trophoblastic, lineage commitment
    Xiaobing Yu
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:461-71. 2008

Collaborators

Detail Information

Publications39

  1. doi request reprint Promise and challenges of human iPSC-based hematologic disease modeling and treatment
    Zhaohui Ye
    Division of Hematology in Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Research Building, Room 1032, Baltimore, MD 21205, USA
    Int J Hematol 95:601-9. 2012
    ..Here, we review the recent development in iPSC-based blood disease modeling, and discuss the unsolved issues and challenges in this new and promising field...
  2. pmc Hematopoietic cells as sources for patient-specific iPSCs and disease modeling
    Zhaohui Ye
    Division of Hematology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Cycle 10:2840-4. 2011
    ..Here we describe the recent advances in generating disease specific human iPSCs from these different types of hematopoietic cells and their potential applications in disease modeling and regenerative medicine...
  3. ncbi request reprint Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells
    Xiaobing Yu
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Department of Oncology, Baltimore, Maryland 21231, USA
    Mol Ther 7:827-38. 2003
    ..Thus, such dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell and will enable many gene transfer applications...
  4. pmc Generation of glycosylphosphatidylinositol anchor protein-deficient blood cells from human induced pluripotent stem cells
    Xuan Yuan
    Division of Hematology, Department of Medicine, School of Medicine, and
    Stem Cells Transl Med 2:819-29. 2013
    ..This conditional PIG-A knockout model should be a valuable tool for studying the importance of GPI-APs in hematopoiesis and human development. ..
  5. pmc Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins
    Guibin Chen
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:345-55. 2008
    ..These data reveal that GPI-AP-enhanced full activation of BMP signaling is required for human trophoblast formation...
  6. ncbi request reprint Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, Maryland 21205, USA
    Stem Cells 26:1998-2005. 2008
    ..Using this improved approach, we also generated hES-like cells from homozygous fibroblasts containing the sickle cell anemia mutation Hemoglobin Sickle. Disclosure of potential conflicts of interest is found at the end of this article...
  7. ncbi request reprint FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cells
    Li Li
    Department of Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA
    Br J Haematol 137:64-75. 2007
    ..CEP-701 may act as a potent therapeutic agent for AML stem cells harbouring FLT3/ITD mutations...
  8. pmc Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders
    Zhaohui Ye
    Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Blood 114:5473-80. 2009
    ..These iPS cells provide a renewable cell source and a prospective hematopoiesis model for investigating MPD pathogenesis...
  9. ncbi request reprint Lentivirus-mediated gene transfer and expression in established human tumor antigen-specific cytotoxic T cells and primary unstimulated T cells
    Xianzheng Zhou
    Division of Immunology and Hematppoiesis, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Hum Gene Ther 14:1089-105. 2003
    ..These results will have significant implications for the study of T-cell biology and for the improvement of clinical gene therapies of acquired immune deficiency syndrome (AIDS) and cancer...
  10. pmc Notch signaling activation in human embryonic stem cells is required for embryonic, but not trophoblastic, lineage commitment
    Xiaobing Yu
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:461-71. 2008
    ..These new insights into the roles of Notch in hESC-fate determination may help to efficiently direct hESC differentiation into therapeutically relevant cell types...
  11. ncbi request reprint Serial imaging of human embryonic stem-cell engraftment and teratoma formation in live mouse models
    Martin G Pomper
    Department of Radiology and Radiological Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA
    Cell Res 19:370-9. 2009
    ..Noninvasive imaging methods such as these may enable us to monitor the presence and distribution of transplanted human stem cells repetitively within live recipients over a long term through the expression of a reporter gene...
  12. pmc Efficient drug screening and gene correction for treating liver disease using patient-specific stem cells
    Su Mi Choi
    Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Hepatology 57:2458-68. 2013
    ....
  13. pmc Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression
    Sarah N Dowey
    Stem Cell Program, Institute for Cell Engineering, Division of Hematology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Protoc 7:2013-21. 2012
    ..We describe here a detailed, validated protocol for effective generation of integration-free human iPSCs from blood MNCs by plasmid vectors...
  14. pmc Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells
    Jizhong Zou
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 5:97-110. 2009
    ..Our study provides the first demonstration of HR-mediated gene targeting in hiPSCs and shows the power of ZFNs for inducing specific genetic modifications in hiPSCs, as well as hESCs...
  15. doi request reprint An improved method for generating and identifying human induced pluripotent stem cells
    Prashant Mali
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 636:191-205. 2010
    ..Finally, we elaborate a robust technique for monitoring and identification of potential iPS cells through live staining of reprogrammed cells. We also outline steps for characterization of the resulting iPS cell lines...
  16. ncbi request reprint Functional antigen-presenting leucocytes derived from human embryonic stem cells in vitro
    Xiangcan Zhan
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Lancet 364:163-71. 2004
    ..We aimed to develop methods to efficiently differentiate hES cells to haemopoietic cells, including immune-modulating leucocytes, a prerequisite of the tolerance induction strategies applying to hES cell-mediated transplantation...
  17. pmc Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent stem cells
    Su Mi Choi
    Stem Cell Biology Laboratory, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Blood 118:1801-5. 2011
    ..The ability to reprogram the widely banked patient B-cell lines will offer an unprecedented opportunity to generate human disease models and provide novel drug therapies...
  18. pmc In vivo functional efficacy of tumor-specific T cells expanded using HLA-Ig based artificial antigen presenting cells (aAPC)
    Malarvizhi Durai
    Department of Pathology, Johns Hopkins School of Medicine, Ross 664G, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Cancer Immunol Immunother 58:209-20. 2009
    ..Thus, our data demonstrate potential therapeutic in vivo activity of HLA-Ig based aAPC expanded CTL to control tumor growth...
  19. pmc Liver engraftment potential of hepatic cells derived from patient-specific induced pluripotent stem cells
    Su Mi Choi
    Stem Cell Biology Laboratory, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Cycle 10:2423-7. 2011
    ....
  20. ncbi request reprint Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer
    Betty Ying Zhou
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, MD 21205, USA
    Stem Cells 25:779-89. 2007
    ..The lentiviral vectors containing the tTS-suppressive, Dox-inducible system offer a universal, inducible, and reversible transgene expression system in essentially any mammalian cell types, including human embryonic stem cells...
  21. pmc Butyrate greatly enhances derivation of human induced pluripotent stem cells by promoting epigenetic remodeling and the expression of pluripotency-associated genes
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Stem Cells 28:713-20. 2010
    ..Moreover, butyrate stimulation provides an efficient method for reprogramming various human adult somatic cells, including cells from patients that are more refractory to reprogramming...
  22. pmc Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures
    Bin Kuan Chou
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cell Res 21:518-29. 2011
    ..This facile method of generating integration-free human iPSCs from blood MNCs will accelerate their use in both research and future clinical applications...
  23. doi request reprint Differential sensitivity to JAK inhibitory drugs by isogenic human erythroblasts and hematopoietic progenitors generated from patient-specific induced pluripotent stem cells
    Zhaohui Ye
    Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Stem Cells 32:269-78. 2014
    ..The iPSC-mediated disease modeling thus underlies the ineffectiveness of the current JAK inhibitors and provides a modeling system to develop better targeted therapies for the JAK2 mutated hematopoiesis...
  24. pmc The phenotype of a germline mutation in PIGA: the gene somatically mutated in paroxysmal nocturnal hemoglobinuria
    Jennifer J Johnston
    Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
    Am J Hum Genet 90:295-300. 2012
    ..We conclude that c.1234C>T in PIGA results in the lethal X-linked phenotype recognized in the reported family...
  25. ncbi request reprint Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells
    Gautam Dravid
    The Institute for Cell Engineering, Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Stem Cells 23:1489-501. 2005
    ..Thus, Wnt/beta-catenin activation does not suffice to maintain the undifferentiated and pluripotent state of hESCs. We propose a new model for the role of Wnt/beta-catenin signaling in undifferentiated hESCs...
  26. ncbi request reprint Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions
    Liuhong Cai
    Institute for Cell Engineering, Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Res 17:62-72. 2007
    ....
  27. ncbi request reprint How reproducible is bioluminescent imaging of tumor cell growth? Single time point versus the dynamic measurement approach
    Shingo Baba
    Division of Nuclear Medicine, The Russell H Morgan Department of Radiology and Radiological Science, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Mol Imaging 6:315-22. 2007
    ..This simplified single time point measurement appears appropriate to estimate the total tumor burden in this model, but the substantial variance at each measurement must be considered in experimental designs...
  28. pmc Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expression
    Linzhao Cheng
    Stem Cell Program in Institute for Cell Engineering and Division of Hematology, Johns Hopkins University, Baltimore, MD 21205, USA
    Cell Stem Cell 10:337-44. 2012
    ..Our data thus suggest that episome-mediated reprogramming is not inherently mutagenic during integration-free iPSC induction...
  29. doi request reprint Lentiviral gene transduction of mouse and human stem cells
    Zhaohui Ye
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD
    Methods Mol Biol 430:243-53. 2008
    ..These cells maintained stable transgene expression after engraftment in mice and in vivo differentiation. Human ESCs can also be transduced with a high efficiency, and transgene is expressed stably after hematopoietic differentiation...
  30. pmc The high-mobility group A1a/signal transducer and activator of transcription-3 axis: an achilles heel for hematopoietic malignancies?
    Joelle Hillion
    Departments of Medicine, Hematology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Cancer Res 68:10121-7. 2008
    ..Our results show that the HMGA1a-STAT3 axis is a potential Achilles heel that could be exploited therapeutically in hematopoietic and other malignancies overexpressing HMGA1a...
  31. ncbi request reprint Molecular imaging and stem cell research
    Yoon Young Jang
    Sidney Kimmel Comprehensive Cancer Center, JohnsHopkins University School of Medicine, Baltimore, MD 21231, USA
    Mol Imaging 10:111-22. 2011
    ..In this review, we discuss the application of current imaging modalities for research of hematopoietic stem cells and pluripotent stem cells and the challenges ahead...
  32. pmc Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors
    Xiaosong Huang
    1 Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 2 Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Mol Ther 22:451-63. 2014
    ..Our result may ultimately lead to an alternative approach to generate unlimited numbers of RBCs for personalized transfusion medicine. ..
  33. pmc Generation of endoderm-derived human induced pluripotent stem cells from primary hepatocytes
    Hua Liu
    Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Hepatology 51:1810-9. 2010
    ..In addition, these cells are able to directly differentiate into definitive endoderm, hepatic progenitors, and mature hepatocytes...
  34. ncbi request reprint Myocyte enhancer factor 2 mediates calcium-dependent transcription of the interleukin-2 gene in T lymphocytes: a calcium signaling module that is distinct from but collaborates with the nuclear factor of activated T cells (NFAT)
    Fan Pan
    Department of Pharmacology and Molecular Science and Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:14477-80. 2004
    ..These results suggest that MEF2 is required for the transcriptional activation of IL-2 and likely other cytokine genes in response to calcium signaling and may serve as a novel target for development of immunosuppressants...
  35. ncbi request reprint Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture
    Linzhao Cheng
    The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Stem Cells 21:131-42. 2003
    ..In addition, this system may help to identify cytokines and adhesion molecules that are required for the self-renewal of hES cells...
  36. pmc In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells
    Nathaniel S Hwang
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 105:20641-6. 2008
    ..In view of the limited available cell sources for tissue engineering applications, these embryonic-derived cells show significant potential in musculoskeletal tissue regeneration applications...
  37. pmc Roles of reactive oxygen species in the fate of stem cells
    Pooja Chaudhari
    Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Antioxid Redox Signal 20:1881-90. 2014
    ..Regulation of ROS has a vital role in maintaining "stemness" and differentiation of the stem cells, as well as in progression of stem-cell-associated diseases...
  38. ncbi request reprint Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells
    Yan Cui
    Division of Immunology and Hematopoiesis, Johns Hopkins Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA
    Blood 99:399-408. 2002
    ..This study demonstrated successful targeting of transgene expression to APCs/DCs after stable gene transduction of pluripotent HSCs...
  39. pmc Potential of human induced pluripotent stem cells derived from blood and other postnatal cell types
    Zhaohui Ye
    Division of Hematology and Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 N Broadway, Baltimore, MD 21205, USA
    Regen Med 5:521-30. 2010
    ..Advantages of blood as a source for reprogramming and applications in regenerative medicine will be discussed...