Kenneth Witwer


Affiliation: Johns Hopkins University School of Medicine
Location: Baltimore, USA


  1. Witwer K. Circulating microRNA biomarker studies: pitfalls and potential solutions. Clin Chem. 2015;61:56-63 pubmed publisher
    ..Careful optimization and standardization of preanalytical and analytical methods is needed to ensure that future results, positive or negative, are reliable. ..
  2. Witwer K, Gama L, Li M, Bartizal C, Queen S, Varrone J, et al. Coordinated regulation of SIV replication and immune responses in the CNS. PLoS ONE. 2009;4:e8129 pubmed publisher
    ..These studies further suggest that interventions should target HIV-infected individuals with increased CCL2 levels or HIV RNA in the CNS. ..
  3. Witwer K, Sisk J, Gama L, Clements J. MicroRNA regulation of IFN-beta protein expression: rapid and sensitive modulation of the innate immune response. J Immunol. 2010;184:2369-76 pubmed publisher
    ..Thus, miRNAs may contribute significantly to the regulation of IFN-beta in innate immune responses. ..
  4. Witwer K, Sarbanes S, Liu J, Clements J. A plasma microRNA signature of acute lentiviral infection: biomarkers of central nervous system disease. AIDS. 2011;25:2057-67 pubmed publisher
  5. Co J, Witwer K, Gama L, Zink M, Clements J. Induction of innate immune responses by SIV in vivo and in vitro: differential expression and function of RIG-I and MDA5. J Infect Dis. 2011;204:1104-14 pubmed publisher
    ..We have shown for the first time to our knowledge the functional role of MDA5 in the innate immune response to SIV infection. ..
  6. Watson A, Witwer K. Do platform-specific factors explain microRNA profiling disparities?. Clin Chem. 2012;58:472-4; author reply 474-5 pubmed publisher
  7. Muth D, McAlexander M, Ostrenga L, Pate N, Izzi J, Adams R, et al. Potential role of cervicovaginal extracellular particles in diagnosis of endometriosis. BMC Vet Res. 2015;11:187 pubmed publisher
  8. Muth D, Powell B, Zhao Z, Witwer K. miRNAs in platelet-poor blood plasma and purified RNA are highly stable: a confirmatory study. BMC Res Notes. 2018;11:273 pubmed publisher
    ..Overall, the repeated observation of miRNA stability is again confirmed. ..
  9. Witwer K, Buchanan E, Myers S, McAlexander M. miRNAs and SAMHD1 regulation in vitro and in a model of HIV CNS disease. J Neuroinflammation. 2015;12:159 pubmed publisher
    ..However, data from an animal model of HIV neurologic disorders may not be consistent with robust miRNA-mediated regulation of SAMHD1 in vivo. ..

More Information


  1. van Dongen H, Masoumi N, Witwer K, Pegtel D. Extracellular Vesicles Exploit Viral Entry Routes for Cargo Delivery. Microbiol Mol Biol Rev. 2016;80:369-86 pubmed publisher
    ..We discuss the challenges ahead in delineating specific roles for EV-associated ligands and cellular receptors. ..
  2. Witwer K, Watson A, Blankson J, Clements J. Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients. Retrovirology. 2012;9:5 pubmed publisher
    ..Using samples from a well-characterized ES cohort, untreated viremic patients, and uninfected controls, we explored the PBMC miRNA profile and probed the relationships of miRNA expression, CD4+ T-cell counts, and viral load...
  3. Liu J, Sisk J, Gama L, Clements J, Witwer K. Tristetraprolin expression and microRNA-mediated regulation during simian immunodeficiency virus infection of the central nervous system. Mol Brain. 2013;6:40 pubmed publisher
  4. Liao Z, Muth D, Eitan E, Travers M, Learman L, Lehrmann E, et al. Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture. Sci Rep. 2017;7:2558 pubmed publisher