DENIS contact WIRTZ

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Resolving the role of actoymyosin contractility in cell microrheology
    Christopher M Hale
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 4:e7054. 2009
  2. pmc Alpha-actinin and filamin cooperatively enhance the stiffness of actin filament networks
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 4:e4411. 2009
  3. pmc A distinctive role for focal adhesion proteins in three-dimensional cell motility
    Stephanie I Fraley
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Nat Cell Biol 12:598-604. 2010
  4. pmc Asymmetric enrichment of PIE-1 in the Caenorhabditis elegans zygote mediated by binary counterdiffusion
    Brian R Daniels
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Cell Biol 184:473-9. 2009
  5. pmc The differential formation of the LINC-mediated perinuclear actin cap in pluripotent and somatic cells
    Shyam B Khatau
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 7:e36689. 2012
  6. pmc Actin cap associated focal adhesions and their distinct role in cellular mechanosensing
    Dong Hwee Kim
    Johns Hopkins Physical Sciences in Oncology Center, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Sci Rep 2:555. 2012
  7. pmc The distinct roles of the nucleus and nucleus-cytoskeleton connections in three-dimensional cell migration
    Shyam B Khatau
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Sci Rep 2:488. 2012
  8. pmc The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction
    Allison B Chambliss
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Sci Rep 3:1087. 2013
  9. pmc A physical sciences network characterization of non-tumorigenic and metastatic cells
    David B Agus
    Center for Applied Molecular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Sci Rep 3:1449. 2013
  10. pmc Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins
    Terrence M Dobrowsky
    1 Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA 2
    Sci Rep 3:3014. 2013

Collaborators

  • Peter C Searson
  • Robert F Siliciano
  • P A Coulombe
  • Alfredo Celedon
  • Yunfeng Feng
  • Linzhao Cheng
  • Donald E Mosier
  • Konstantinos Konstantopoulos
  • Osigwe Esue
  • Sam Walcott
  • Jerry S H Lee
  • Henry Higgs
  • Ismo Virtanen
  • Sarah E Hitchcock-DeGregori
  • Michael A Chernousov
  • G Danuser
  • David Kovar
  • R S Fischer
  • Yiider Tseng
  • Sean X Sun
  • Shyam B Khatau
  • Thomas P Kole
  • Brian R Daniels
  • Terrence M Dobrowsky
  • Porntula Panorchan
  • Christopher M Hale
  • Gregory D Longmore
  • Didier Hodzic
  • Saumendra Bajpai
  • Dong Hwee Kim
  • Wei Chiang Chen
  • Allison B Chambliss
  • Pei Hsun Wu
  • Justin Hanes
  • Bridget Wildt
  • Samuel K Lai
  • P J Stewart-Hutchinson
  • Anjil Giri
  • Stephanie I Fraley
  • Erdinc Atilgan
  • Junghae Suh
  • Melissa S Thompson
  • Soichiro Yamada
  • David B Agus
  • Sharon Gerecht
  • Benjamin W Schafer
  • Steven C Almo
  • Ranjini Krishnamurthy
  • Edward M Perkins
  • Ying Ying Wang
  • Colin L Stewart
  • Xiaoming Zhou
  • Stephanie L Gupton
  • Ingjye Jiang
  • Joseph L Katz
  • Michael G Klein
  • William D Hanley
  • Steven R Heidemann
  • Michelle Dawson
  • Hoi Yeung Li
  • William Hanley
  • Amit Rahman
  • Jingyuan Xu
  • Alan Goodman
  • Casey M Kraning-Rush
  • S Alireza Rabi
  • Jae Min Choi
  • Robert A Gatenby
  • Joseph E Aslan
  • JAN T LIPHARDT
  • Parag Mallick
  • Barbara L Hempstead
  • Jonathan D Licht
  • Owen J T McCarty
  • Mark C Lloyd
  • Carl Kesselman
  • Alexander Fuhrmann
  • Paolo Decuzzi
  • Guillaume Lambert
  • Biana Godin
  • Jack R Staunton
  • Yolanda Stypula
  • Robert H Austin
  • Michael L Shuler
  • Hariharan Subramanian
  • Courtney Hemphill
  • Liyu Liu
  • William M Grady
  • Brian Kwee
  • Rebecca Nedellec

Detail Information

Publications71

  1. pmc Resolving the role of actoymyosin contractility in cell microrheology
    Christopher M Hale
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 4:e7054. 2009
    ....
  2. pmc Alpha-actinin and filamin cooperatively enhance the stiffness of actin filament networks
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 4:e4411. 2009
    ..Here we hypothesize that alpha-actinin and filamin, two major F-actin crosslinking proteins that are both present in the lamella of adherent cells, display synergistic mechanical functions...
  3. pmc A distinctive role for focal adhesion proteins in three-dimensional cell motility
    Stephanie I Fraley
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Nat Cell Biol 12:598-604. 2010
    ..This study shows that membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3D matrix...
  4. pmc Asymmetric enrichment of PIE-1 in the Caenorhabditis elegans zygote mediated by binary counterdiffusion
    Brian R Daniels
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Cell Biol 184:473-9. 2009
    ....
  5. pmc The differential formation of the LINC-mediated perinuclear actin cap in pluripotent and somatic cells
    Shyam B Khatau
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 7:e36689. 2012
    ..While, the localization of lamin A/C at the nuclear envelope is required for perinuclear actin cap formation, it is not sufficient to control nuclear shape...
  6. pmc Actin cap associated focal adhesions and their distinct role in cellular mechanosensing
    Dong Hwee Kim
    Johns Hopkins Physical Sciences in Oncology Center, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Sci Rep 2:555. 2012
    ..These results establish the perinuclear actin cap and associated ACAFAs as major mediators of cellular mechanosensing and a critical element of the physical pathway that transduce mechanical cues all the way to the nucleus...
  7. pmc The distinct roles of the nucleus and nucleus-cytoskeleton connections in three-dimensional cell migration
    Shyam B Khatau
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Sci Rep 2:488. 2012
    ..A simple mechanical model explains why LINC complexes and the perinuclear actin cap are essential in 3D migration by providing mechanical support to the formation of pseudopodial protrusions...
  8. pmc The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction
    Allison B Chambliss
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Sci Rep 3:1087. 2013
    ..These results suggest an interconnected physical pathway for mechanotransduction, from the extracellular milieu to the nucleus...
  9. pmc A physical sciences network characterization of non-tumorigenic and metastatic cells
    David B Agus
    Center for Applied Molecular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Sci Rep 3:1449. 2013
    ....
  10. pmc Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins
    Terrence M Dobrowsky
    1 Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA 2
    Sci Rep 3:3014. 2013
    ..Env proteins can better mediate entry into cells after coreceptor switch; this effective entry capacity does not correlate with the bond strengths between viral Env and cellular receptors...
  11. pmc Organization of cellular receptors into a nanoscale junction during HIV-1 adhesion
    Terrence M Dobrowsky
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS Comput Biol 6:e1000855. 2010
    ..The formation of an organized viral junction under multiple physiopathologically relevant conditions may represent a novel intermediate step in productive infection...
  12. pmc The Arp2/3 complex mediates multigeneration dendritic protrusions for efficient 3-dimensional cancer cell migration
    Anjil Giri
    1D W, Johns Hopkins University, 3400 North Charles St, Croft Hall, Baltimore, MD 21218, USA
    FASEB J 27:4089-99. 2013
    ..The relative contribution of these proteins to 3D migration is significantly different from their role in 2D migration...
  13. pmc Simultaneously defining cell phenotypes, cell cycle, and chromatin modifications at single-cell resolution
    Allison B Chambliss
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    FASEB J 27:2667-76. 2013
    ....
  14. pmc Focal adhesion size uniquely predicts cell migration
    Dong Hwee Kim
    Johns Hopkins Physical Sciences Oncology Center and Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    FASEB J 27:1351-61. 2013
    ..This study suggests that the mean size of focal adhesions robustly and precisely predicts cell speed independently of focal adhesion surface density and molecular composition...
  15. pmc Functional interplay between the cell cycle and cell phenotypes
    Wei Chiang Chen
    Johns Hopkins Physical Sciences Oncology Center, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Integr Biol (Camb) 5:523-34. 2013
    ..This study introduces a high-throughput method to simultaneously measure the cell cycle and phenotypes at single-cell resolution, which reveals a complex functional interplay between the cell cycle and cell phenotypes...
  16. pmc The physics of cancer: the role of physical interactions and mechanical forces in metastasis
    Denis Wirtz
    Department of Materials Science and Engineering, Institute for NanoBioTechnology, Johns Hopkins Center of Cancer Nanotechnology Excellence, Johns Hopkins Physical Sciences in Oncology Center, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Nat Rev Cancer 11:512-22. 2011
    ..The emerging insight into these physical interactions may help to solve some long-standing questions in disease progression and may lead to new approaches to developing cancer diagnostics and therapies...
  17. doi request reprint Particle-tracking microrheology of living cells: principles and applications
    Denis Wirtz
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Annu Rev Biophys 38:301-26. 2009
    ..Particle-tracking microrheology can readily reveal the lost ability of diseased cells to resist shear forces...
  18. pmc Triggering cell detachment from patterned electrode arrays by programmed subcellular release
    Bridget Wildt
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    Nat Protoc 5:1273-80. 2010
    ..Fabrication of the electrode arrays may take 1-2 d. Preparation for experiments, including surface functionalization and cell plating, can be completed in 10 h. A series of cell release experiments on one device may last several hours...
  19. pmc Rho kinase regulates the intracellular micromechanical response of adherent cells to rho activation
    Thomas P Kole
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Mol Biol Cell 15:3475-84. 2004
    ..Our results reveal fundamental differences between intracellular elasticity and cellular tension and suggest a critical role for Rho kinase in the regulation of intracellular mechanics...
  20. pmc Micromechanical mapping of live cells by multiple-particle-tracking microrheology
    Yiider Tseng
    Department of Chemical Engineering, The Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    Biophys J 83:3162-76. 2002
    ..MPTM is a new type of functional microscopy that can test the local, rate-dependent mechanical and ultrastructural properties of living cells...
  21. doi request reprint Chapter 18: Sensing cytoskeletal mechanics by ballistic intracellular nanorheology (BIN) coupled with cell transfection
    Melissa S Thompson
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Methods Cell Biol 89:467-86. 2008
    ....
  22. ncbi request reprint Micromechanical coupling between cell surface receptors and RGD peptides
    Amit Rahman
    Department of Chemical Engineering, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    Biochem Biophys Res Commun 296:771-8. 2002
    ....
  23. pmc Cdc42 mediates nucleus movement and MTOC polarization in Swiss 3T3 fibroblasts under mechanical shear stress
    Jerry S H Lee
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Mol Biol Cell 16:871-80. 2005
    ....
  24. pmc Programmed subcellular release for studying the dynamics of cell detachment
    Bridget Wildt
    Department of Materials Science and Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    Nat Methods 6:211-3. 2009
    ..Here we report programmed subcellular release, a method for spatially and temporally controlled cellular detachment, and present quantitative results of the detachment dynamics of 3T3 fibroblasts at the subcellular level...
  25. pmc Microrheology and ROCK signaling of human endothelial cells embedded in a 3D matrix
    Porntula Panorchan
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biophys J 91:3499-507. 2006
    ..These results suggest that ROCK plays an essential role in the regulation of the intracellular mechanical response to VEGF of endothelial cells in a 3D matrix...
  26. ncbi request reprint Ballistic intracellular nanorheology reveals ROCK-hard cytoplasmic stiffening response to fluid flow
    Jerry S H Lee
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    J Cell Sci 119:1760-8. 2006
    ....
  27. pmc Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization, and migration
    Jerry S H Lee
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 93:2542-52. 2007
    ..These results also suggest that cell polarization during cell migration requires tight mechanical coupling between MTOC and nucleus, which is mediated by lamin A/C...
  28. pmc Dysfunctional connections between the nucleus and the actin and microtubule networks in laminopathic models
    Christopher M Hale
    Department of Chemical and Biomolecular Engineering, Washington University School of Medicine, St Louis, Missouri, USA
    Biophys J 95:5462-75. 2008
    ....
  29. pmc A perinuclear actin cap regulates nuclear shape
    Shyam B Khatau
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 106:19017-22. 2009
    ..These results highlight the interplay between cell shape, nuclear shape, and cell adhesion mediated by the perinuclear actin cap...
  30. doi request reprint The filamentous actin cross-linking/bundling activity of mammalian formins
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    J Mol Biol 384:324-34. 2008
    ....
  31. pmc Altering mucus rheology to "solidify" human mucus at the nanoscale
    Samuel K Lai
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, USA
    PLoS ONE 4:e4294. 2009
    ..These findings provide important insight into the nanoscale structural and barrier properties of mucus, and how the penetration of foreign particles across mucus might be inhibited...
  32. ncbi request reprint Effect of length, topology, and concentration on the microviscosity and microheterogeneity of DNA solutions
    Alan Goodman
    Department of Chemical Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    J Mol Biol 323:199-215. 2002
    ..These in vitro results suggest that the topology, local concentration, and length of DNA influence the microrheology and microheterogeneity of the DNA within the nucleus...
  33. pmc MEX-5 enrichment in the C. elegans early embryo mediated by differential diffusion
    Brian R Daniels
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Development 137:2579-85. 2010
    ..This work extends the scope of reaction/diffusion models to include not only germline morphogens, but also somatic determinants...
  34. pmc Pairwise assembly determines the intrinsic potential for self-organization and mechanical properties of keratin filaments
    Soichiro Yamada
    Department of Chemical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Mol Biol Cell 13:382-91. 2002
    ..Our findings underscore the fundamental importance of pairwise polymerization and have implications for the functional significance of keratin sequence diversity...
  35. ncbi request reprint The rapid onset of elasticity during the assembly of the bacterial cell-division protein FtsZ
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    Biochem Biophys Res Commun 333:508-16. 2005
    ..These studies suggest that FtsZ filaments have the toughness to provide strong mechanical support for the maintenance and circumferential constriction of the bacterial Z-ring...
  36. ncbi request reprint Probing cellular mechanical responses to stimuli using ballistic intracellular nanorheology
    Porntula Panorchan
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Methods Cell Biol 83:115-40. 2007
    ..For instance, BIN allows us to probe an ensemble of cells embedded deeply inside a three-dimensional extracellular matrix or as a monolayer of cells subjected to shear flows...
  37. ncbi request reprint A direct interaction between actin and vimentin filaments mediated by the tail domain of vimentin
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 281:30393-9. 2006
    ....
  38. ncbi request reprint Receptor-ligand binding: 'catch' bonds finally caught
    Konstantinos Konstantopoulos
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    Curr Biol 13:R611-3. 2003
    ..Recent research reveals that P-selectin binding to its ligand exhibits 'catch' to 'slip' bond transition that may help explain the shear threshold phenomenon...
  39. pmc Mechanics and dynamics of actin-driven thin membrane protrusions
    Erdinc Atilgan
    Department of Mechanical Engineering and the Whitaker Institute of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 90:65-76. 2006
    ..Filopodia also attract each other through distortions of the membrane. Spatially close filopodia will merge to form a larger one. Force-velocity relationships mimicking micromanipulation experiments testing our predictions are computed...
  40. pmc Probing single-cell micromechanics in vivo: the microrheology of C. elegans developing embryos
    Brian R Daniels
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biophys J 90:4712-9. 2006
    ..These results support the hypothesis that the asymmetric positioning of the mitotic spindle stems from an asymmetric distribution of elementary force generators as opposed to asymmetric viscosity of the cytoplasm...
  41. ncbi request reprint Enhanced viscoelasticity of human cystic fibrotic sputum correlates with increasing microheterogeneity in particle transport
    Michelle Dawson
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 278:50393-401. 2003
    ....
  42. ncbi request reprint Single molecule characterization of P-selectin/ligand binding
    William Hanley
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 278:10556-61. 2003
    ....
  43. ncbi request reprint The bimodal role of filamin in controlling the architecture and mechanics of F-actin networks
    Yiider Tseng
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    J Biol Chem 279:1819-26. 2004
    ..These results resolve the controversy by showing that F-actin/filamin networks can adopt diametrically opposed rheological behaviors depending on the concentration in cross-linking proteins...
  44. pmc A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo
    Hoi Yeung Li
    Howard Hughes Medical Institute, Dept of Embryology, Carnegie Institution of Washington, 115 W University Pkwy, Baltimore, MD 21210, USA
    J Cell Biol 160:635-44. 2003
    ..Successful nucleotide exchange dissociates the binary complex, permitting the release of RCC1 and RanGTP from the chromatin and the production of RanGTP on the chromatin surface...
  45. ncbi request reprint Functional synergy of actin filament cross-linking proteins
    Yiider Tseng
    Department of Chemical Engineering and Graduate Program in Molecular Biophysics, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 277:25609-16. 2002
    ..Our studies support a re-evaluation of the notion of functional redundancy among cytoskeletal regulatory proteins...
  46. pmc Efficient active transport of gene nanocarriers to the cell nucleus
    Junghae Suh
    Department of Biomedical Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 100:3878-82. 2003
    ....
  47. pmc Loss of alpha-catenin decreases the strength of single E-cadherin bonds between human cancer cells
    Saumendra Bajpai
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 284:18252-9. 2009
    ....
  48. ncbi request reprint Distinct kinetic and mechanical properties govern selectin-leukocyte interactions
    William D Hanley
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Cell Sci 117:2503-11. 2004
    ....
  49. ncbi request reprint Real-time intracellular transport of gene nanocarriers studied by multiple particle tracking
    Junghae Suh
    Department of Biomedical Engineering, The Johns Hopkins University, 720 Rutland Ave, Baltimore, Maryland 21205, USA
    Biotechnol Prog 20:598-602. 2004
    ..Multiple particle tracking characterizes gene carrier transport in complex biological environments and, therefore, may be a useful tool in quantifying rate-limiting steps in gene delivery within cells and other biological media...
  50. doi request reprint Chapter 15: Live-cell single-molecule force spectroscopy
    Terrence M Dobrowsky
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Methods Cell Biol 89:411-32. 2008
    ....
  51. pmc Micro- and macrorheology of mucus
    Samuel K Lai
    Department of Chemical and Biomolecular Engineering JH Primary Appointment, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    Adv Drug Deliv Rev 61:86-100. 2009
    ....
  52. pmc Morphology of the lamellipodium and organization of actin filaments at the leading edge of crawling cells
    Erdinc Atilgan
    Department of Mechanical Engineering and the Whitaker Institute of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 89:3589-602. 2005
    ..These features, combined with the mechanical properties of the cell membrane, explain why lamellipodium is a flat organelle...
  53. ncbi request reprint Single-molecule analysis of cadherin-mediated cell-cell adhesion
    Porntula Panorchan
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    J Cell Sci 119:66-74. 2006
    ..Together these results suggest that N-cadherin and E-cadherin molecules form homophilic bonds between juxtaposed cells that have significantly different kinetic and micromechanical properties...
  54. pmc GTPase activity, structure, and mechanical properties of filaments assembled from bacterial cytoskeleton protein MreB
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, University The Johns Hopkins University, Baltimore, MD 21218, USA
    J Bacteriol 188:968-76. 2006
    ..maritima MreB filaments, including high rigidity and propensity to form bundles, suggest a mechanism by which MreB helical structure may be involved in imposing a cylindrical architecture on rod-shaped bacterial cells...
  55. ncbi request reprint The mechanical properties of simple epithelial keratins 8 and 18: discriminating between interfacial and bulk elasticities
    Soichiro Yamada
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    J Struct Biol 143:45-55. 2003
    ..The potential for modulation of mechanical properties through self-organization may be a general property of keratin polymers and contribute to their organization and function in vivo...
  56. pmc {alpha}-Catenin mediates initial E-cadherin-dependent cell-cell recognition and subsequent bond strengthening
    Saumendra Bajpai
    Department of Chemical and Biomolecular Engineering, and Howard Hughes Medical Institute Graduate Training Program and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Proc Natl Acad Sci U S A 105:18331-6. 2008
    ....
  57. pmc Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin
    Stephanie L Gupton
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 168:619-31. 2005
    ..Inhibition of endogenous long TM isoforms alters protrusion persistence. Thus, cells can migrate with inhibited lamellipodia, and we suggest that TM is a major regulator of F-actin functional specialization in migrating cells...
  58. ncbi request reprint Mechanical shear can accelerate the gelation of actin filament networks
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Phys Rev Lett 95:048301. 2005
    ..This effect of shear-induced strengthening of polymerizing networks depends on the state of hydrolysis of the actin-bound adenosine triphosphate...
  59. ncbi request reprint Micro-organization and visco-elasticity of the interphase nucleus revealed by particle nanotracking
    Yiider Tseng
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, 3400 N Charles Street, MD 21218, USA
    J Cell Sci 117:2159-67. 2004
    ....
  60. ncbi request reprint How actin crosslinking and bundling proteins cooperate to generate an enhanced cell mechanical response
    Yiider Tseng
    Department of Chemical and Biomolecular Engineering and Program in Molecular Biophysics, The Johns Hopkins University, Baltimore, MD 21218, USA
    Biochem Biophys Res Commun 334:183-92. 2005
    ..Thus the combination of structural proteins with similar function can provide the cell with unique properties that are required for biologically optimal responses...
  61. pmc Intracellular mechanics of migrating fibroblasts
    Thomas P Kole
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Mol Biol Cell 16:328-38. 2005
    ..We conclude that a polarized mechanics of the cytoskeleton is essential for directed cell migration and is coordinated through microtubules...
  62. pmc Fibronectin fibrillogenesis regulates three-dimensional neovessel formation
    Xiaoming Zhou
    The Division of Molecular Medicine and Genetics, Department of Internal Medicine, The Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA
    Genes Dev 22:1231-43. 2008
    ..These results advance a new model wherein Fn polymerization serves as a structural scaffolding that displays adhesive ligands on a mechanically ideal substratum for promoting neovessel development...
  63. pmc Mechanics of enveloped virus entry into host cells
    Sean X Sun
    Biophys J 90:L10-2. 2006
    ..Resistance to engulfment is dominated by the elastic deformation of the cytoskeleton...
  64. pmc Structural requirements for the assembly of LINC complexes and their function in cellular mechanical stiffness
    P J Stewart-Hutchinson
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Exp Cell Res 314:1892-905. 2008
    ....
  65. ncbi request reprint Intracellular microrheology as a tool for the measurement of the local mechanical properties of live cells
    Thomas P Kole
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Methods Cell Biol 78:45-64. 2004
  66. ncbi request reprint Towards a regional approach to cell mechanics
    Steven R Heidemann
    Department of Physiology, Michigan State University, East Lansing, MI 48824 3320, USA
    Trends Cell Biol 14:160-6. 2004
    ..that such physical studies would be of more interest to most cell biologists if greater emphasis were placed on the well-established regional differences within a cell and the ability of the cell to quickly change its mechanical behaviors..
  67. ncbi request reprint The assembly of MreB, a prokaryotic homolog of actin
    Osigwe Esue
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 280:2628-35. 2005
    ..These findings suggest that despite high structural homology, MreB and actin display significantly different assembly properties...
  68. ncbi request reprint Structure of the actin crosslinking core of fimbrin
    Michael G Klein
    Department of Biochemistry, Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Structure 12:999-1013. 2004
    ..These results underscore the power of jointly considering structural and genetic suppressor data for obtaining unexpected and biologically relevant mechanistic information...
  69. ncbi request reprint Dendritic branching and homogenization of actin networks mediated by arp2/3 complex
    Yiider Tseng
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles Street, Baltimore, Maryland 21218, USA
    Phys Rev Lett 93:258104. 2004
    ..These results suggest that the Arp2/3 complex possesses a unique function of stabilizing membrane protrusions through the formation of homogeneous and stiff actin cytoskeleton at the leading edge of crawling cells...
  70. ncbi request reprint Nuclear envelope breakdown requires overcoming the mechanical integrity of the nuclear lamina
    Porntula Panorchan
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 279:43462-7. 2004
    ..These results demonstrate that both mechanical tearing of the lamina and biochemical modification of lamin B1 filaments are required for NE breakdown...
  71. ncbi request reprint Microheterogeneity and microrheology of wheat gliadin suspensions studied by multiple-particle tracking
    Jingyuan Xu
    Biomaterials Processing Research, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, Illinois 61604, USA
    Biomacromolecules 3:92-9. 2002
    ....

Research Grants6

  1. The nucleus-cytoskeleton connection in health and disease
    Denis Wirtz; Fiscal Year: 2009
    ..The proposed research drawing from bioengineering and cell biology may help establish a biophysical basis for the wide variety of disease phenotypes associated to human laminopathies. ..
  2. Magnetic quantum dots for active sensing of the nuclear envelope
    Denis Wirtz; Fiscal Year: 2007
    ..3d. We will investigate whether and how the actin and microtubule networks affect the mechanical properties of the NE. ..
  3. The nucleus-cytoskeleton connection in health and disease
    DENIS contact WIRTZ; Fiscal Year: 2010
    ..The proposed research drawing from bioengineering and cell biology may help establish a biophysical basis for the wide variety of disease phenotypes associated to human laminopathies. ..
  4. The nucleus-cytoskeleton connection in health and disease
    Denis Wirtz; Fiscal Year: 2009
    ..The proposed research drawing from bioengineering and cell biology may help establish a biophysical basis for the wide variety of disease phenotypes associated to human laminopathies. ..