L A Shimoda

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Hypoxia. 4. Hypoxia and ion channel function
    Larissa A Shimoda
    Div of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Cell Physiol 300:C951-67. 2011
  2. pmc Activation of hypoxia-inducible factor-1 in pulmonary arterial smooth muscle cells by endothelin-1
    Sarah Pisarcik
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 304:L549-61. 2013
  3. pmc 55th Bowditch Lecture: Effects of chronic hypoxia on the pulmonary circulation: role of HIF-1
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Appl Physiol 113:1343-52. 2012
  4. pmc Vascular remodeling in pulmonary hypertension
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University, Baltimore, MD 21224, USA
    J Mol Med (Berl) 91:297-309. 2013
  5. pmc Hypoxia-induced migration in pulmonary arterial smooth muscle cells requires calcium-dependent upregulation of aquaporin 1
    Kyle Leggett
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, JHAAC 4A 52, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 303:L343-53. 2012
  6. pmc Kinase-dependent activation of voltage-gated Ca2+ channels by ET-1 in pulmonary arterial myocytes during chronic hypoxia
    Trevor Luke
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 302:L1128-39. 2012
  7. ncbi request reprint Acute and chronic hypoxic pulmonary vasoconstriction: a central role for endothelin-1?
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, JHAAC 4A 52, Baltimore, MD 21224, USA
    Respir Physiol Neurobiol 132:93-106. 2002
  8. ncbi request reprint HIF-1 regulates hypoxic induction of NHE1 expression and alkalinization of intracellular pH in pulmonary arterial myocytes
    Larissa A Shimoda
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, JHAAC 4A 52, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 291:L941-9. 2006
  9. ncbi request reprint Inhibition of hypoxia-induced calcium responses in pulmonary arterial smooth muscle by acetazolamide is independent of carbonic anhydrase inhibition
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 292:L1002-12. 2007
  10. pmc Interactions between calcium and reactive oxygen species in pulmonary arterial smooth muscle responses to hypoxia
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21224, United States
    Respir Physiol Neurobiol 174:221-9. 2010

Collaborators

Detail Information

Publications42

  1. pmc Hypoxia. 4. Hypoxia and ion channel function
    Larissa A Shimoda
    Div of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Cell Physiol 300:C951-67. 2011
    ..In this review, we describe the effects of both acute and sustained hypoxia (continuous and intermittent) on mammalian ion channels in several tissues, the mode of action, and their contribution to diverse cellular processes...
  2. pmc Activation of hypoxia-inducible factor-1 in pulmonary arterial smooth muscle cells by endothelin-1
    Sarah Pisarcik
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 304:L549-61. 2013
    ....
  3. pmc 55th Bowditch Lecture: Effects of chronic hypoxia on the pulmonary circulation: role of HIF-1
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Appl Physiol 113:1343-52. 2012
    ..Current work is focused on the regulation of HIF in the chronically hypoxic lung and evaluation of the potential for pharmacological inhibitors of HIF to prevent, reverse, or slow the progression of pulmonary hypertension...
  4. pmc Vascular remodeling in pulmonary hypertension
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University, Baltimore, MD 21224, USA
    J Mol Med (Berl) 91:297-309. 2013
    ....
  5. pmc Hypoxia-induced migration in pulmonary arterial smooth muscle cells requires calcium-dependent upregulation of aquaporin 1
    Kyle Leggett
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, JHAAC 4A 52, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 303:L343-53. 2012
    ..Silencing AQP1 via siRNA also prevented hypoxia-induced migration of PASMCs. Our results suggest that hypoxia induces a PASMC-specific increase in [Ca(2+)](i) that results in increased AQP1 protein levels and cell migration...
  6. pmc Kinase-dependent activation of voltage-gated Ca2+ channels by ET-1 in pulmonary arterial myocytes during chronic hypoxia
    Trevor Luke
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 302:L1128-39. 2012
    ..These results suggest that following CH, the ET-1-induced Δ[Ca(2+)](i) in PASMCs occurs via Ca(2+) influx through VDCCs mediated primarily by PKC, TKs, and Rho kinase...
  7. ncbi request reprint Acute and chronic hypoxic pulmonary vasoconstriction: a central role for endothelin-1?
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, JHAAC 4A 52, Baltimore, MD 21224, USA
    Respir Physiol Neurobiol 132:93-106. 2002
    ....
  8. ncbi request reprint HIF-1 regulates hypoxic induction of NHE1 expression and alkalinization of intracellular pH in pulmonary arterial myocytes
    Larissa A Shimoda
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, JHAAC 4A 52, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 291:L941-9. 2006
    ....
  9. ncbi request reprint Inhibition of hypoxia-induced calcium responses in pulmonary arterial smooth muscle by acetazolamide is independent of carbonic anhydrase inhibition
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 292:L1002-12. 2007
    ..These results suggest that AZ attenuates HPV by selectively inhibiting hypoxia-induced Ca2+ responses via a mechanism independent of CA inhibition, changes in pH(i), or membrane potential...
  10. pmc Interactions between calcium and reactive oxygen species in pulmonary arterial smooth muscle responses to hypoxia
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21224, United States
    Respir Physiol Neurobiol 174:221-9. 2010
    ..This review will discuss the mechanisms regulating [Ca2+]i and ROS in PASMCs, and the interaction between ROS and Ca2+ signaling during exposure to acute hypoxia...
  11. ncbi request reprint Inhibition of inwardly rectifying K(+) channels by cGMP in pulmonary vascular endothelial cells
    Larissa A Shimoda
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 283:L297-304. 2002
    ..The depolarization could be a protective mechanism that prevents endothelial cell barrier dysfunction by reducing the driving force for Ca(2+) entry...
  12. ncbi request reprint Inhibition of voltage-gated K(+) currents by endothelin-1 in human pulmonary arterial myocytes
    L A Shimoda
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 281:L1115-22. 2001
    ..Our results are consistent with data we reported previously in the rat, suggesting similar mechanisms may be operative in both species...
  13. ncbi request reprint Altered pulmonary vasoreactivity in the chronically hypoxic lung
    L A Shimoda
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School of Medicine Baltimore, Maryland 21224, USA
    Physiol Res 49:549-60. 2000
    ....
  14. ncbi request reprint Mobilization of intracellular Ca(2+) by endothelin-1 in rat intrapulmonary arterial smooth muscle cells
    L A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 278:L157-64. 2000
    ..These results suggest that ET-1 initiates depolarization in PASMCs, leading to Ca(2+) influx through voltage-gated Ca(2+) channels and Ca(2+) release from ryanodine- and inositol 1,4,5-trisphosphate-sensitive stores...
  15. ncbi request reprint Effects of hypoxia in porcine pulmonary arterial myocytes: roles of K(V) channel and endothelin-1
    J S Sham
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 279:L262-72. 2000
    ..These effects cannot be fully explained by inhibition of K(V) channels and were greatly enhanced via synergistic interactions with the endothelium-derived factor endothelin-1...
  16. ncbi request reprint Partial HIF-1alpha deficiency impairs pulmonary arterial myocyte electrophysiological responses to hypoxia
    L A Shimoda
    Division of Pulmonary and Critical Care Medicine and Institute of Genetic Medicine, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 281:L202-8. 2001
    ..Thus partial deficiency of HIF-1alpha is sufficient to impair hypoxia-induced depolarization, reduction of K+ current density, and PASMC hypertrophy...
  17. ncbi request reprint Hypoxic constriction of porcine distal pulmonary arteries: endothelium and endothelin dependence
    Q Liu
    Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 280:L856-65. 2001
    ....
  18. ncbi request reprint Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle
    Jian Wang
    Div of Pulmonary and Critical Care Medicine, The Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Cir, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 286:L848-58. 2004
    ..Our results suggest that CCE through TRPC-encoded Ca2+ channels could contribute to Ca2+ signaling in myocytes from distal intrapulmonary arteries...
  19. ncbi request reprint Chronic hypoxia elevates intracellular pH and activates Na+/H+ exchange in pulmonary arterial smooth muscle cells
    Eon J Rios
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins Univ, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 289:L867-74. 2005
    ..Our findings indicate that exposure to CH caused increased pHi, NHE activity, and NHE1 expression, changes that may contribute to the development of pulmonary hypertension, in part, via pH-dependent induction of PASMC proliferation...
  20. ncbi request reprint L-type Ca(2+) channels, resting [Ca(2+)](i), and ET-1-induced responses in chronically hypoxic pulmonary myocytes
    L A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 279:L884-94. 2000
    ....
  21. ncbi request reprint Inhibition of hypoxic pulmonary vasoconstriction by antagonists of store-operated Ca2+ and nonselective cation channels
    Letitia Weigand
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 289:L5-L13. 2005
    ....
  22. ncbi request reprint Acute hypoxia increases intracellular [Ca2+] in pulmonary arterial smooth muscle by enhancing capacitative Ca2+ entry
    Jian Wang
    Div of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Physiol Lung Cell Mol Physiol 288:L1059-69. 2005
    ..Our results suggest that acute hypoxia enhances CCE through SOCCs in distal PASMCs, leading to depolarization, secondary activation of VOCCs, and increased [Ca2+]i. SOCCs and CCE may play important roles in HPV...
  23. ncbi request reprint Ca2+ signaling in hypoxic pulmonary vasoconstriction: effects of myosin light chain and Rho kinase antagonists
    Jian Wang
    Division of Pulmonary and Critical Care Medicine, The Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 293:L674-85. 2007
    ....
  24. ncbi request reprint Endothelin-1 mediates hypoxia-induced inhibition of voltage-gated K+ channel expression in pulmonary arterial myocytes
    E Miles Whitman
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 294:L309-18. 2008
    ..These results suggest that the inhibition of K(v) channel expression and rise in [Ca(2+)](i) during chronic hypoxia may be the result of HIF-1-dependent induction of ET-1...
  25. ncbi request reprint ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors
    Wei min Zhang
    Div of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 285:L680-90. 2003
    ....
  26. ncbi request reprint Physiological properties and functions of Ca(2+) sparks in rat intrapulmonary arterial smooth muscle cells
    Carmelle V Remillard
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 283:L433-44. 2002
    ..However, they play a different modulatory role on membrane potential and are under agonist-specific regulation independent of global [Ca(2+)]...
  27. ncbi request reprint Effect of cGMP on lung microvascular endothelial barrier dysfunction following hydrogen peroxide
    David B Pearse
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland 21224, USA
    Endothelium 10:309-17. 2003
    ..These results suggest that in BLMVECs, cGMP countered the adverse effects of H(2)O(2) on barrier function by preventing actin cytoskeletal rearrangement and attenuating the increase in [Ca(2+)](i)...
  28. ncbi request reprint Chronic hypoxia inhibits Kv channel gene expression in rat distal pulmonary artery
    Jian Wang
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Physiol Lung Cell Mol Physiol 288:L1049-58. 2005
    ....
  29. ncbi request reprint Hypoxia inducible factor 1 mediates hypoxia-induced TRPC expression and elevated intracellular Ca2+ in pulmonary arterial smooth muscle cells
    Jian Wang
    Division of Pulmonary and Critical Care Medicine, Institute for Cell Engineering, Baltimore, MD, USA
    Circ Res 98:1528-37. 2006
    ....
  30. ncbi request reprint Hypoxic constriction and reactive oxygen species in porcine distal pulmonary arteries
    J Q Liu
    Division of Pulmonary and Critical Care Medicine, The Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 285:L322-33. 2003
    ..We conclude that HPV required increased concentrations of ROS produced by and acting on pulmonary arterial smooth muscle rather than endothelium...
  31. ncbi request reprint Ca2+ channels and chronic hypoxia
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21224, USA
    Microcirculation 13:657-70. 2006
    ..We will also highlight future directions of investigation that might improve our understanding of the response of pulmonary vascular cells to chronic hypoxia...
  32. pmc Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia
    Wenju Lu
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 295:L104-13. 2008
    ..These results provide further support for the importance of SOCE in HPV and suggest that HPV is greater in distal than proximal PA because greater numbers and activation of SOCC in distal PASMC generate bigger increases in [Ca(2+)](i)...
  33. ncbi request reprint Mechanisms of endothelin-1-induced contraction in pulmonary arteries from chronically hypoxic rats
    Letitia Weigand
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins Univ, 5501 Hopkins Bayview Circle, JHAAC 4B 82B, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 290:L284-90. 2006
    ..We conclude that the component of ET-1-induced contraction that occurs without a change in [Ca2+]i in IPA from CH rats requires activation of Rho kinase and TKs, but not PKC...
  34. pmc Impaired physiological responses to chronic hypoxia in mice partially deficient for hypoxia-inducible factor 1alpha
    A Y Yu
    Institute of Genetic Medicine, Division of Pulmonary and Critical Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 3914, USA
    J Clin Invest 103:691-6. 1999
    ..These results indicate that partial HIF-1alpha deficiency has significant effects on multiple systemic responses to chronic hypoxia...
  35. ncbi request reprint Functional analysis of the role of hypoxia-inducible factor 1 in the pathogenesis of hypoxic pulmonary hypertension
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkinds University School of Medicine, Baltimore, Maryland 21224, USA
    Methods Enzymol 381:121-9. 2004
  36. ncbi request reprint Role of protein kinase G in barrier-protective effects of cGMP in human pulmonary artery endothelial cells
    Aigul Moldobaeva
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Hopkins Bayview Medical Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 290:L919-30. 2006
    ....
  37. doi request reprint Hypoxic regulation of ion channels and transporters in pulmonary vascular smooth muscle
    Larissa A Shimoda
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA
    Adv Exp Med Biol 661:221-35. 2010
    ....
  38. pmc Knockdown of stromal interaction molecule 1 attenuates store-operated Ca2+ entry and Ca2+ responses to acute hypoxia in pulmonary arterial smooth muscle
    Wenju Lu
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 297:L17-25. 2009
    ....
  39. ncbi request reprint Hypoxia decreases expression of soluble guanylate cyclase in cultured rat pulmonary artery smooth muscle cells
    Paul M Hassoun
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Respir Cell Mol Biol 30:908-13. 2004
    ....
  40. pmc Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia
    Huafeng Zhang
    Vascular Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 283:10892-903. 2008
    ....
  41. pmc Role of the TGF-beta/Alk5 signaling pathway in monocrotaline-induced pulmonary hypertension
    Ari L Zaiman
    Johns Hopkins School of Medicine, Division of Pulmonary and Critical Care Medicine, 1830 East Monument Street, 5th Floor, Baltimore, MD 21205, USA
    Am J Respir Crit Care Med 177:896-905. 2008
    ..However, the role of TGF-beta family signaling in PH and pulmonary vascular remodeling remains unclear...
  42. pmc Exon-based mapping of microarray probes: recovering differential gene expression signal in underpowered hypoxia experiment
    Dmitry N Grigoryev
    Johns Hopkins University, School of Medicine, Baltimore, MD, USA
    Mol Cell Probes 21:134-9. 2007
    ..This method will facilitate re-evaluation of existing underpowered Affymetrix gene expression profiles...