Ie Ming Shih

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Identification of molecular pathway aberrations in uterine serous carcinoma by genome-wide analyses
    Elisabetta Kuhn
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    J Natl Cancer Inst 104:1503-13. 2012
  2. pmc Genome-wide identification of significant aberrations in cancer genome
    Xiguo Yuan
    School of Computer Science and Technology, Xidian University, Xi an, P R China
    BMC Genomics 13:342. 2012
  3. pmc The diagnostic and biological implications of laminin expression in serous tubal intraepithelial carcinoma
    Elisabetta Kuhn
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 36:1826-34. 2012
  4. pmc Knowledge-guided multi-scale independent component analysis for biomarker identification
    Li Chen
    Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State University, Arlington, VA, USA
    BMC Bioinformatics 9:416. 2008
  5. ncbi request reprint Molecular basis of gestational trophoblastic diseases
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institution, Baltimore, Maryland 21287 6917, USA
    Curr Mol Med 2:1-12. 2002
  6. pmc Application of human leukocyte antigen-G expression in the diagnosis of human cancer
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Hum Immunol 68:272-6. 2007
  7. ncbi request reprint Trophogram, an immunohistochemistry-based algorithmic approach, in the differential diagnosis of trophoblastic tumors and tumorlike lesions
    Ie Ming Shih
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Ann Diagn Pathol 11:228-34. 2007
  8. pmc Pathogenesis of ovarian cancer: clues from selected overexpressed genes
    Ie Ming Shih
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21212, USA
    Future Oncol 5:1641-57. 2009
  9. ncbi request reprint Molecular pathogenesis of ovarian borderline tumors: new insights and old challenges
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Clin Cancer Res 11:7273-9. 2005
  10. ncbi request reprint p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations
    I Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 28:1177-83. 2004

Research Grants

  1. The Roles of HBXAP Gene in Ovarian Cancer
    Ie Ming Shih; Fiscal Year: 2010
  2. Molecular Diagnostics for Malignant Effusion
    Ie Ming Shih; Fiscal Year: 2010
  3. Molecular Diagnostics for Malignant Effusion
    Ie Ming Shih; Fiscal Year: 2006
  4. Development of Digital SNP Analysis in Cancer Detection
    Ie Ming Shih; Fiscal Year: 2005
  5. Molecular Diagnostics for Malignant Effusion
    Ie Ming Shih; Fiscal Year: 2011

Collaborators

Detail Information

Publications135 found, 100 shown here

  1. pmc Identification of molecular pathway aberrations in uterine serous carcinoma by genome-wide analyses
    Elisabetta Kuhn
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    J Natl Cancer Inst 104:1503-13. 2012
    ..However, the molecular pathogenesis of uterine serous carcinoma is largely unknown. We analyzed the genomes of uterine serous carcinoma samples to better understand the molecular genetic characteristics of this cancer...
  2. pmc Genome-wide identification of significant aberrations in cancer genome
    Xiguo Yuan
    School of Computer Science and Technology, Xidian University, Xi an, P R China
    BMC Genomics 13:342. 2012
    ....
  3. pmc The diagnostic and biological implications of laminin expression in serous tubal intraepithelial carcinoma
    Elisabetta Kuhn
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 36:1826-34. 2012
    ....
  4. pmc Knowledge-guided multi-scale independent component analysis for biomarker identification
    Li Chen
    Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State University, Arlington, VA, USA
    BMC Bioinformatics 9:416. 2008
    ..In this paper, we develop a novel strategy, namely knowledge-guided multi-scale independent component analysis (ICA), to first infer regulatory signals and then identify biologically relevant biomarkers from microarray data...
  5. ncbi request reprint Molecular basis of gestational trophoblastic diseases
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institution, Baltimore, Maryland 21287 6917, USA
    Curr Mol Med 2:1-12. 2002
    ..This review will focus on these advances with special emphasis on the pathogenesis of each specific form of GTD. In addition, the morphology and clinical behavior of each of these entities will be briefly discussed...
  6. pmc Application of human leukocyte antigen-G expression in the diagnosis of human cancer
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Hum Immunol 68:272-6. 2007
    ..Specifically, the promises and challenges for applying HLA-G expression to detect cancer in body fluids, to diagnose different types of human cancer and to predict clinical outcome in cancer patients will be briefly reviewed...
  7. ncbi request reprint Trophogram, an immunohistochemistry-based algorithmic approach, in the differential diagnosis of trophoblastic tumors and tumorlike lesions
    Ie Ming Shih
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Ann Diagn Pathol 11:228-34. 2007
    ..The trophogram may prove to be useful in assisting the differential diagnosis of various trophoblastic diseases in diagnostic pathology...
  8. pmc Pathogenesis of ovarian cancer: clues from selected overexpressed genes
    Ie Ming Shih
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21212, USA
    Future Oncol 5:1641-57. 2009
    ..Since the study of ovarian cancer-associated genes is complicated by several factors unique to ovarian cancer, we will also present our views on the limitations and challenges of current ovarian cancer research...
  9. ncbi request reprint Molecular pathogenesis of ovarian borderline tumors: new insights and old challenges
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Clin Cancer Res 11:7273-9. 2005
    ..This review summarizes recent molecular studies of ovarian borderline tumors with special emphasis on the role of SBT in tumor progression and its relationship to ovarian serous carcinoma...
  10. ncbi request reprint p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations
    I Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 28:1177-83. 2004
    ..Recognizing these distinctive expression patterns helps to further elucidate the biology of trophoblast and can also provide a useful tool for the differential diagnosis of trophoblastic lesions...
  11. pmc Distinct DNA methylation profiles in ovarian serous neoplasms and their implications in ovarian carcinogenesis
    Ie Ming Shih
    Division of Gynecologic Pathology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Am J Obstet Gynecol 203:584.e1-22. 2010
    ..The purpose of this study was to analyze DNA methylation profiles among different types of ovarian serous neoplasm, which is a task that has not been performed...
  12. doi request reprint Trophoblastic vasculogenic mimicry in gestational choriocarcinoma
    Ie Ming Shih
    Departments of Pathology, Oncology and Gynecology Obstetrics, Johns Hopkins Medical Institutions, 1550 Orleans Street, Baltimore, MD 21231, USA
    Mod Pathol 24:646-52. 2011
    ..These findings provide cogent evidence that choriocarcinoma represents one of a few human tumor types that utilizes vasculogenic mimicry by tumor cell in supporting tumor development...
  13. pmc Amplification of the ch19p13.2 NACC1 locus in ovarian high-grade serous carcinoma
    Ie Ming Shih
    Departments of Pathology, Oncology and Gynecology Obstetrics, Johns Hopkins Medical Institutions, 1550 Orleans Street, Baltimore, MD 21231, USA
    Mod Pathol 24:638-45. 2011
    ..005). In summary, our data suggest that amplification at the ch19p13.2 NACC1 locus, leading to NAC1 overexpression, is one of the molecular genetic alterations associated with early tumor recurrence in ovarian cancer...
  14. pmc Somatic mutations of PPP2R1A in ovarian and uterine carcinomas
    Ie Ming Shih
    Department of Gynecology and Obstetrics, Johns Hopkins Medical Institutions, Baltimore, MD21231, USA
    Am J Pathol 178:1442-7. 2011
    ....
  15. ncbi request reprint The Role of E-cadherin in the Motility and Invasion of Implantation Site Intermediate Trophoblast
    Ie Ming Shih
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, USA
    Placenta 23:706-15. 2002
    ....
  16. doi request reprint Mutation of PPP2R1A: a new clue in unveiling the pathogenesis of uterine serous carcinoma
    Ie Ming Shih
    Department of Pathology, Oncology, Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    J Pathol 224:1-4. 2011
    ....
  17. pmc Power of the eternal youth: Nanog expression in the gestational choriocarcinoma
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Pathol 173:911-4. 2008
  18. ncbi request reprint Characterization of active mitogen-activated protein kinase in ovarian serous carcinomas
    Chih Yi Hsu
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Clin Cancer Res 10:6432-6. 2004
    ..This study evaluates the expression of active MAPK in ovarian serous carcinomas, with response to treatment and survival...
  19. pmc Frequent mutations of chromatin remodeling gene ARID1A in ovarian clear cell carcinoma
    Sian Jones
    Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA
    Science 330:228-31. 2010
    ..In a total of 42 OCCCs, 7% had mutations in PPP2R1A and 57% had mutations in ARID1A. These results suggest that aberrant chromatin remodeling contributes to the pathogenesis of OCCC...
  20. pmc Mutation and loss of expression of ARID1A in uterine low-grade endometrioid carcinoma
    Bin Guan
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Surg Pathol 35:625-32. 2011
    ....
  21. ncbi request reprint Amplicon profiles in ovarian serous carcinomas
    Kentaro Nakayama
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA
    Int J Cancer 120:2613-7. 2007
    ..Taken together, our results provide the first comprehensive analysis of DNA copy number changes in highly pure ovarian serous carcinoma. These findings may have important biological and clinical implications...
  22. pmc Functional analysis of in-frame indel ARID1A mutations reveals new regulatory mechanisms of its tumor suppressor functions
    Bin Guan
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Neoplasia 14:986-93. 2012
    ....
  23. pmc DNA copy numbers profiles in affinity-purified ovarian clear cell carcinoma
    Kuan Ting Kuo
    Division of Gynecological Pathology, Departments of Pathology, Gynecology Obstetrics, and Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Clin Cancer Res 16:1997-2008. 2010
    ..The purpose of this study is to comprehensively define the genomic changes in CCC based on DNA copy number alterations...
  24. ncbi request reprint HLA-G is a potential tumor marker in malignant ascites
    Gad Singer
    Department of Pathology, Johns Hopkins University Medical Institutions, Baltimore Maryland 21231, USA
    Clin Cancer Res 9:4460-4. 2003
    ..HLA-G is a potential tumor-associated marker and that one of its isoforms, HLA-G5, produces a secretory protein. This study is to assess the clinical utility of secreted HLA-G levels in differential diagnosis of malignant ascites...
  25. pmc A BTB/POZ protein, NAC-1, is related to tumor recurrence and is essential for tumor growth and survival
    Kentaro Nakayama
    Department of Pathology, Johns Hopkins Medical Institutions, 1550 Orleans Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 103:18739-44. 2006
    ....
  26. ncbi request reprint Expression of Rsf-1, a chromatin-remodeling gene, in ovarian and breast carcinoma
    Tsui Lien Mao
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Hum Pathol 37:1169-75. 2006
    ..Because Rsf-1 overexpression occurs in only a small number of breast carcinomas, it is unlikely that Rsf-1 is a critical gene in the development of breast carcinoma...
  27. pmc Amplification of a chromatin remodeling gene, Rsf-1/HBXAP, in ovarian carcinoma
    Ie Ming Shih
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 102:14004-9. 2005
    ..Furthermore, Rsf-1 gene knockdown inhibited cell growth in OVCAR3 cells, which harbor Rsf-1 amplification. Taken together, these findings indicate an important role of Rsf-1 amplification in ovarian cancer...
  28. ncbi request reprint Assessment of plasma DNA levels, allelic imbalance, and CA 125 as diagnostic tests for cancer
    Hsueh Wei Chang
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    J Natl Cancer Inst 94:1697-703. 2002
    ..AI can be detected in genomic tumor DNA released into the blood after necrosis or apoptosis. We evaluated plasma DNA concentration, allelic status in plasma DNA, and serum CA 125 level as screening tests for ovarian and other cancers...
  29. doi request reprint TP53 mutations in serous tubal intraepithelial carcinoma and concurrent pelvic high-grade serous carcinoma--evidence supporting the clonal relationship of the two lesions
    Elisabetta Kuhn
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    J Pathol 226:421-6. 2012
    ..Specifically, strong diffuse staining correlates with a missense mutation, whereas complete absence of staining correlates with null mutations...
  30. ncbi request reprint Notch3 gene amplification in ovarian cancer
    Joon T Park
    Department of Gynecology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 66:6312-8. 2006
    ..These results indicate that Notch3 is required for proliferation and survival of Notch3-amplified tumors and inactivation of Notch3 can be a potential therapeutic approach for ovarian carcinomas...
  31. ncbi request reprint Inactivation of the mitogen-activated protein kinase pathway as a potential target-based therapy in ovarian serous tumors with KRAS or BRAF mutations
    Gudrun Pohl
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 65:1994-2000. 2005
    ....
  32. doi request reprint Rsf-1, a chromatin remodelling protein, interacts with cyclin E1 and promotes tumour development
    Jim Jinn Chyuan Sheu
    Department of Pathology, Gynecology and Obstetrics and Oncology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    J Pathol 229:559-68. 2013
    ..The above findings suggest that Rsf-1 interacts and collaborates with cyclin E1 in neoplastic transformation and TP53 mutations are a prerequisite for tumour-promoting functions of the RSF/cyclin E1 complex...
  33. pmc Serous tubal intraepithelial carcinoma upregulates markers associated with high-grade serous carcinomas including Rsf-1 (HBXAP), cyclin E and fatty acid synthase
    Ann Smith Sehdev
    Department of Pathology, Legacy Health Systems, Portland, OR, USA
    Mod Pathol 23:844-55. 2010
    ..In contrast, mucin-4 immunoreactivity either did not change or was reduced in most STICs. These results suggest that overexpression of Rsf-1, cyclin E and FASN occurs early in tumor progression...
  34. pmc Frequent activating mutations of PIK3CA in ovarian clear cell carcinoma
    Kuan Ting Kuo
    Departments of Pathology, Howard Hughes Medical Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Am J Pathol 174:1597-601. 2009
    ..We therefore suggest that the use of PIK3CA-targeting drugs may offer a more effective therapeutic approach compared with current chemotherapeutic agents for patients with advanced-stage and recurrent CCC...
  35. pmc Trophoblastic neoplasms express fatty acid synthase, which may be a therapeutic target via its inhibitor C93
    Stefanie M Ueda
    Kelly Gynecologic Oncology Service, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Am J Pathol 175:2618-24. 2009
    ..FASN inhibitors such as C93 warrant further investigation as targeted therapeutic agents for metastatic and chemoresistant gestational trophoblastic neoplasia...
  36. pmc Loss of ARID1A expression correlates with stages of tumor progression in uterine endometrioid carcinoma
    Tsui Lien Mao
    Department of Pathology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD Seirei Mikatahara General Hospital, Hamamatsu, Japan
    Am J Surg Pathol 37:1342-8. 2013
    ..These findings suggest that loss of ARID1A expression, presumably due to mutation, plays an important role in tumor progression of uterine endometrioid carcinoma. ..
  37. ncbi request reprint NAC-1 controls cell growth and survival by repressing transcription of Gadd45GIP1, a candidate tumor suppressor
    Kentaro Nakayama
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Cancer Res 67:8058-64. 2007
    ..In summary, NAC-1 contributes to tumor growth and survival by at least inhibiting Gadd45GIP1 expression, which has a tumor suppressor effect in cancer cells...
  38. pmc Notch3 overexpression is related to the recurrence of ovarian cancer and confers resistance to carboplatin
    Joon Tae Park
    Departments of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Am J Pathol 177:1087-94. 2010
    ..Taken together, the above findings suggest that Notch3 pathway activation reprograms tumor cells to assume an array of embryonic stem cell markers and participates in development of chemoresistance in HGSC...
  39. ncbi request reprint Identifying tumor origin using a gene expression-based classification map
    Phillip Buckhaults
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 63:4144-9. 2003
    ..This expression map analysis may provide a reliable and practical approach to determine tumor type in cases of metastatic carcinoma of clinically unknown origin...
  40. ncbi request reprint Detection of allelic imbalance in ascitic supernatant by digital single nucleotide polymorphism analysis
    Hsueh Wei Chang
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Clin Cancer Res 8:2580-5. 2002
    ..In this study we determined the presence of allelic imbalance (AI) in tumor-released DNA in ascitic supernatant by directly counting the alleles using a newly developed technology, digital single nucleotide polymorphism (SNP) analysis...
  41. pmc ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers
    Bin Guan
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 71:6718-27. 2011
    ..Our results provide functional evidence in support of the hypothesis that ARID1A is a bona fide tumor suppressor that collaborates with p53 to regulate CDKN1A and SMAD3 transcription and tumor growth in gynecologic cancers...
  42. pmc Functional analysis of 11q13.5 amplicon identifies Rsf-1 (HBXAP) as a gene involved in paclitaxel resistance in ovarian cancer
    Jung Hye Choi
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 69:1407-15. 2009
    ..In conclusion, our results suggest that Rsf-1 is the major gene within the 11q13.5 amplicon that contributes to paclitaxel resistance, and the formation of the Rsf-1/hSNF2H complex is required for inducing this phenotype...
  43. pmc Rsf-1, a chromatin remodeling protein, induces DNA damage and promotes genomic instability
    Jim Jinn Chyuan Sheu
    Department of Pathology and Oncology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    J Biol Chem 285:38260-9. 2010
    ..The above findings suggest that increased Rsf-1 expression and thus excessive RSF activity, which occurs in tumors harboring Rsf-1 amplification, can induce chromosomal instability likely through DDR...
  44. doi request reprint Utility of p16 expression for distinction of uterine serous carcinomas from endometrial endometrioid and endocervical adenocarcinomas: immunohistochemical analysis of 201 cases
    Anna Yemelyanova
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Surg Pathol 33:1504-14. 2009
    ....
  45. ncbi request reprint Expression of L-selectin ligands in human endometrium during the implantation window after controlled ovarian stimulation for oocyte donation
    Tsung Hsuan Lai
    Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Fertil Steril 85:761-3. 2006
    ..Controlled ovarian stimulation, using the antagonist protocol in this study, is associated with a reduction of L-selectin ligand expression during the implantation window which may adversely affect the endometrial environment...
  46. ncbi request reprint Increased plasma DNA integrity in cancer patients
    Brant G Wang
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 63:3966-8. 2003
    ..59. Our findings suggest that increased DNA integrity in plasma DNA is associated with cancer, and measurement of DNA integrity may provide a simple and inexpensive measure for cancer detection...
  47. ncbi request reprint Mutations in BRAF and KRAS characterize the development of low-grade ovarian serous carcinoma
    Gad Singer
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    J Natl Cancer Inst 95:484-6. 2003
    ..The apparent restriction of these BRAF and KRAS mutations to low-grade serous ovarian carcinoma and its precursors suggests that low-grade and high-grade ovarian serous carcinomas develop through independent pathways...
  48. pmc Loss of ARID1A expression is an early molecular event in tumor progression from ovarian endometriotic cyst to clear cell and endometrioid carcinoma
    Ayse Ayhan
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Int J Gynecol Cancer 22:1310-5. 2012
    ....
  49. ncbi request reprint Expression of extracellular matrix proteins in ovarian serous tumors
    Ritu Salani
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Int J Gynecol Pathol 26:141-6. 2007
    ..007). In summary, ovarian carcinomas exhibit up-regulated expression of several extracellular matrix proteins, and syndecan 1 represents a novel tumor-associated marker in ovarian serous carcinomas...
  50. pmc Defining NOTCH3 target genes in ovarian cancer
    Xu Chen
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 72:2294-303. 2012
    ..Our findings define direct target genes of NOTCH3 and highlight the role of DLGAP5 in mediating the function of NOTCH3...
  51. pmc The origin and pathogenesis of epithelial ovarian cancer: a proposed unifying theory
    Robert J Kurman
    Departments of Pathology, Gynecology and Obstetrics and Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Surg Pathol 34:433-43. 2010
    ..Appreciation of these new concepts will allow for a more rationale approach to screening, treatment, and prevention that potentially can have a significant impact on reducing the mortality of this devastating disease...
  52. doi request reprint Shortened telomeres in serous tubal intraepithelial carcinoma: an early event in ovarian high-grade serous carcinogenesis
    Elisabetta Kuhn
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 34:829-36. 2010
    ....
  53. ncbi request reprint Identification and characterization of membralin, a novel tumor-associated gene, in ovarian carcinoma
    Yu Chi Chen
    Department of Pathology and Oncology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Biochim Biophys Acta 1730:96-102. 2005
    ..001). In conclusion, these findings represent the first characterization of human membralin and suggest that membralin is a novel tumor-associated marker in ovarian serous carcinomas...
  54. doi request reprint Ovarian Brenner tumour: a morphologic and immunohistochemical analysis suggesting an origin from fallopian tube epithelium
    Elisabetta Kuhn
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD, USA Electronic address
    Eur J Cancer 49:3839-49. 2013
    ..Brenner tumours (BTs), like other epithelial ovarian tumours, are thought to develop from the ovarian surface epithelium...
  55. ncbi request reprint Diffuse mesothelin expression correlates with prolonged patient survival in ovarian serous carcinoma
    M Jim Yen
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Clin Cancer Res 12:827-31. 2006
    ..In this study, we correlate mesothelin immunoreactivity to clinicopathologic features in ovarian serous carcinoma...
  56. ncbi request reprint Ubiquitin-proteasome system stress sensitizes ovarian cancer to proteasome inhibitor-induced apoptosis
    Martina Bazzaro
    Department of Pathology, The Johns Hopkins School of Medicine, Cancer Research Building 2, 1550 Orleans Street, Baltimore, MD 21231, USA
    Cancer Res 66:3754-63. 2006
    ..In sum, elevated proliferation and metabolic rate resulting from malignant transformation of the epithelium stresses the UPS and renders ovarian carcinoma more sensitive to apoptosis in response to proteasomal inhibition...
  57. ncbi request reprint Immunohistochemistry of choriocarcinoma: an aid in differential diagnosis and in elucidating pathogenesis
    Tsui Lien Mao
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 31:1726-32. 2007
    ....
  58. ncbi request reprint Annexin XI is associated with cisplatin resistance and related to tumor recurrence in ovarian cancer patients
    Jin Song
    Department of Pathology, Johns Hopkins Medical Institutions, 1550 Orleans Street, Baltimore, MD 21231, USA
    Clin Cancer Res 13:6842-9. 2007
    ..In this study, a protein identified to be associated with cisplatin resistance in ovarian cancer cells was investigated in ovarian cancer tissues to address its clinical significance...
  59. ncbi request reprint Mutations of BRAF and KRAS precede the development of ovarian serous borderline tumors
    Chung Liang Ho
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 64:6915-8. 2004
    ..Our findings provide cogent evidence that mutations of BRAF and KRAS occur in the epithelium of cystadenomas adjacent to SBTs and strongly suggest that they are very early events in tumorigenesis, preceding the development of SBT...
  60. ncbi request reprint HLA-G immunoreactivity is specific for intermediate trophoblast in gestational trophoblastic disease and can serve as a useful marker in differential diagnosis
    Gad Singer
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Am J Surg Pathol 26:914-20. 2002
    ..In conclusion, HLA-G immunoreactivity appears to be specific for IT in gestational trophoblastic disease and can serve as a useful marker in the differential diagnosis of these lesions...
  61. ncbi request reprint Nuclear size distinguishes low- from high-grade ovarian serous carcinoma and predicts outcome
    Chih Yi Hsu
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Hum Pathol 36:1049-54. 2005
    ..In addition, MNA is an independent prognostic factor for high-grade serous carcinoma...
  62. ncbi request reprint Molecular genetic analysis of ovarian serous cystadenomas
    Eric J Cheng
    Department of Pathology, The Johns Hopkins University, Baltimore, MD 21231, USA
    Lab Invest 84:778-84. 2004
    ..Accordingly, it appears that serous cystadenomas develop as a hyperplastic expansion from epithelial inclusions with a clonal/neoplastic transformation occurring in a subset of them...
  63. pmc Validation of an algorithm for the diagnosis of serous tubal intraepithelial carcinoma
    Russell Vang
    Departments of Pathology, Division of Gynecologic Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Int J Gynecol Pathol 31:243-53. 2012
    ..Accordingly, we recommend its use in research studies. Before applying it to routine clinical practice, the algorithm should be evaluated by general surgical pathologists in a community setting...
  64. pmc Endocervical-type mucinous borderline tumors are related to endometrioid tumors based on mutation and loss of expression of ARID1A
    Chen Hsuan Wu
    Departments of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Int J Gynecol Pathol 31:297-303. 2012
    ..Accordingly, we suggest designating these tumors "atypical proliferative (borderline) papillary müllerian tumors" as this designation more accurately reflects their clinicopathologic, immunohistochemical, and molecular genetic features...
  65. doi request reprint HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions
    Tsui Lien Mao
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 32:236-42. 2008
    ..In conclusion, as compared with other trophoblastic markers, HSD3B1 is highly specific and sensitive, being expressed in all types of trophoblastic lesions but not in a variety of nontrophoblastic tumors of the uterus, lung, and breast...
  66. ncbi request reprint Sequence mutations and amplification of PIK3CA and AKT2 genes in purified ovarian serous neoplasms
    Kentaro Nakayama
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Biol Ther 5:779-85. 2006
    ....
  67. pmc Low-grade serous carcinoma of the ovary displaying a macropapillary pattern of invasion
    Anna Yemelyanova
    Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD 21231, USA
    Am J Surg Pathol 32:1800-6. 2008
    ..The recognition of this pattern is important, especially in cases when a tumor is composed entirely of macropapillae...
  68. ncbi request reprint Apolipoprotein E is required for cell proliferation and survival in ovarian cancer
    Yu Chi Chen
    Department of Pathology, Johns Hopkins University Medical Institutions, Baltimore, MD 21231, USA
    Cancer Res 65:331-7. 2005
    ..004). This study suggests a new role of apoE in cancer as apoE expression is important for the proliferation and survival in apoE-expressing ovarian cancer cells...
  69. ncbi request reprint Homozygous deletion of MKK4 in ovarian serous carcinoma
    Kentaro Nakayama
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Biol Ther 5:630-4. 2006
    ..These findings suggest that homozygous deletion or reduced expression of MKK4 may contribute to the development of ovarian serous carcinoma...
  70. pmc Low-grade serous carcinomas of the ovary contain very few point mutations
    Sian Jones
    Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA
    J Pathol 226:413-20. 2012
    ..Our mutational analysis demonstrates that point mutations are much less common in low-grade serous tumours of the ovary than in other adult tumours, a finding with interesting scientific and clinical implications...
  71. pmc Proteomic analysis of temporally stimulated ovarian cancer cells for biomarker discovery
    Mark A Marzinke
    Department of Pathology, Johns Hopkins University, Baltimore, MD 21231, USA
    Mol Cell Proteomics 12:356-68. 2013
    ..LAMP-1 is a promising biomarker for studies of the progression of EGF-stimulated ovarian cancers and might be useful in predicting treatment responses involving tyrosine kinase inhibitors or EGF receptor monoclonal antibodies...
  72. pmc NAC1 is an actin-binding protein that is essential for effective cytokinesis in cancer cells
    Kai Lee Yap
    Department of Pathology, Pathobiology Graduate Program, Oncology Center, Johns Hopkins University, Baltimore, Maryland 21231, USA
    Cancer Res 72:4085-96. 2012
    ..Taken together, our results indicate that the NAC1/actin/profilin-1 complex is crucial for cancer cell cytokinesis, with a variety of important biologic and clinical implications...
  73. pmc Activation of mitogen-activated protein kinase is required for migration and invasion of placental site trophoblastic tumor
    Martin Köbel
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Am J Pathol 167:879-85. 2005
    ..In contrast, neither compound had any effect on normal extravillous trophoblastic cells or JEG-3 cells. In conclusion, our findings demonstrate a functional role of MAPK activation in the motility and invasion of PSTT...
  74. pmc Defining the cut point between low-grade and high-grade ovarian serous carcinomas: a clinicopathologic and molecular genetic analysis
    Ayse Ayhan
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Am J Surg Pathol 33:1220-4. 2009
    ....
  75. ncbi request reprint The development of high-grade serous carcinoma from atypical proliferative (borderline) serous tumors and low-grade micropapillary serous carcinoma: a morphologic and molecular genetic analysis
    Reiko Dehari
    Department of Pathology, Gynecology and Obstetrics, Johns Hopkins Medical Institutions, Baltimore, MD, USA
    Am J Surg Pathol 31:1007-12. 2007
    ..The above results suggest that the majority of high-grade and low-grade carcinomas develop independently but in rare cases, a high-grade serous carcinoma may arise from an atypical proliferative serous (borderline) tumor...
  76. doi request reprint Subdividing ovarian and peritoneal serous carcinoma into moderately differentiated and poorly differentiated does not have biologic validity based on molecular genetic and in vitro drug resistance data
    Russell Vang
    Department of Pathology, Division of Gynecologic Pathology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21231, USA
    Am J Surg Pathol 32:1667-74. 2008
    ..Accordingly, they can be simply classified as high-grade serous carcinoma...
  77. pmc Papillary tubal hyperplasia: the putative precursor of ovarian atypical proliferative (borderline) serous tumors, noninvasive implants, and endosalpingiosis
    Robert J Kurman
    Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD, USA
    Am J Surg Pathol 35:1605-14. 2011
    ..If this hypothesis is confirmed, it can be concluded that low-grade and high-grade ovarian tumors develop from tubal epithelium and involve the ovary secondarily...
  78. doi request reprint Are all pelvic (nonuterine) serous carcinomas of tubal origin?
    Christopher G Przybycin
    Departments of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Am J Surg Pathol 34:1407-16. 2010
    ....
  79. doi request reprint Telomere length in different histologic types of ovarian carcinoma with emphasis on clear cell carcinoma
    Elisabetta Kuhn
    Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MA 21231, USA
    Mod Pathol 24:1139-45. 2011
    ....
  80. ncbi request reprint Measurement of cyclin E genomic copy number and strand length in cell-free DNA distinguish malignant versus benign effusions
    Ritu Salani
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Clin Cancer Res 13:5805-9. 2007
    ..We hypothesized that analysis of both DNA copy number and strand length of cell-free DNA from an amplified chromosomal region could improve the diagnosis of malignant diseases in body fluids...
  81. pmc Identification of PBX1 target genes in cancer cells by global mapping of PBX1 binding sites
    Michelle M Thiaville
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, United States of America
    PLoS ONE 7:e36054. 2012
    ..These results demonstrate that MEOX1 is a critical target gene and cofactor of PBX1 in ovarian cancers...
  82. pmc Analysis of DNA copy number alterations in ovarian serous tumors identifies new molecular genetic changes in low-grade and high-grade carcinomas
    Kuan Ting Kuo
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Cancer Res 69:4036-42. 2009
    ..Our study provides a genome-wide homozygous deletion profile in HG serous carcinomas, which can serve as a molecular foundation to study tumor suppressors in ovarian cancer...
  83. ncbi request reprint Differential expression of L-selectin ligand in the endometrium during the menstrual cycle
    Tsung Hsuan Lai
    Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Fertil Steril 83:1297-302. 2005
    ..To evaluate the expression of L-selectin ligand in the endometrium during the menstrual cycle...
  84. pmc Comparison of candidate serologic markers for type I and type II ovarian cancer
    Dan Lu
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Gynecol Oncol 122:560-6. 2011
    ..To examine the value of individual and combinations of ovarian cancer associated blood biomarkers for the discrimination between plasma of patients with type I or II ovarian cancer and disease-free volunteers...
  85. ncbi request reprint Ovarian cancer specific kallikrein profile in effusions
    Ie Ming Shih
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Gynecol Oncol 105:501-7. 2007
    ..However, a comprehensive and parallel analysis of different secreted kallikreins in ovarian cancer has not been performed. This study was undertaken to profile the secreted kallikreins in cancer effusion supernatants...
  86. pmc Early detection and treatment of ovarian cancer: shifting from early stage to minimal volume of disease based on a new model of carcinogenesis
    Robert J Kurman
    Department of Pathology, Bloomberg School of Public Health, The Johns Hopkins Medical Institutions, Baltimore, MD, USA
    Am J Obstet Gynecol 198:351-6. 2008
    ..A more rational approach to early detection of ovarian cancer should focus on low volume rather than low stage of disease...
  87. pmc Myosin II co-chaperone general cell UNC-45 overexpression is associated with ovarian cancer, rapid proliferation, and motility
    Martina Bazzaro
    Department of Pathology, The Johns Hopkins School of Medicine, Baltimore, Maryland, USA
    Am J Pathol 171:1640-9. 2007
    ..In sum, these findings implicate elevated GC UNC-45 protein expression in ovarian carcinoma proliferation and metastasis...
  88. pmc MicroRNA expression and identification of putative miRNA targets in ovarian cancer
    Neetu Dahiya
    Laboratory of Cellular and Molecular Biology, Hopkins Medical Institutions, Baltimore, Maryland, United States of America
    PLoS ONE 3:e2436. 2008
    ..Emerging evidence suggests the potential involvement of altered regulation of miRNA in the pathogenesis of cancers, and these genes are thought to function as both tumor suppressors and oncogenes...
  89. pmc Clinical and biological significance of HLA-G expression in ovarian cancer
    Jim Jinn Chyuan Sheu
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA
    Semin Cancer Biol 17:436-43. 2007
    ....
  90. pmc Pathogenesis of ovarian cancer: lessons from morphology and molecular biology and their clinical implications
    Robert J Kurman
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Int J Gynecol Pathol 27:151-60. 2008
    ..The model does not replace the histopathologic classification but, by drawing attention to the molecular genetic events that play a role in tumor progression, sheds light on new approaches to early detection and treatment...
  91. pmc Oncoproteomic analysis reveals co-upregulation of RELA and STAT5 in carboplatin resistant ovarian carcinoma
    Natini Jinawath
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, United States of America
    PLoS ONE 5:e11198. 2010
    ..Elucidating the pathogenesis underlying drug resistance is fundamental to the development of new therapeutics, leading to improved clinical outcomes in these patients...
  92. pmc Jagged-1 and Notch3 juxtacrine loop regulates ovarian tumor growth and adhesion
    Jung Hye Choi
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Cancer Res 68:5716-23. 2008
    ....
  93. pmc Rsf-1 (HBXAP) expression is associated with advanced stage and lymph node metastasis in ovarian clear cell carcinoma
    Daichi Maeda
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Int J Gynecol Pathol 30:30-5. 2011
    ..Our data suggest that Rsf-1 participates in tumor progression in CCC, and indicates that the contribution of Rsf-1 to disease aggressiveness deserves further study...
  94. ncbi request reprint Identification of BCOX1, a novel gene overexpressed in breast cancer
    Jin Song
    Department of Pathology, Johns Hopkins Medical Institutions, 417 N Caroline Str, Baltimore, MD 21231, USA
    Biochim Biophys Acta 1760:62-9. 2006
    ..This study warrants further investigations to explore the molecular functions of BCOX1, and to determine its potential diagnostic and therapeutic applications for breast cancer...
  95. pmc Jagged1 expression regulated by Notch3 and Wnt/β-catenin signaling pathways in ovarian cancer
    Xu Chen
    Department of Gynecology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Oncotarget 1:210-8. 2010
    ..In addition, Wnt/β-catenin pathway activation also up-regulated Jagged1. Both mechanisms may sustain Notch3 signaling in ovarian cancer cells and contribute to the pathogenesis of ovarian carcinoma...
  96. pmc Suppression of annexin A11 in ovarian cancer: implications in chemoresistance
    Jin Song
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Neoplasia 11:605-14, 1 p following 614. 2009
    ..Collectively, annexin A11 is directly involved in cell proliferation and cisplatin resistance of ovarian cancer. Manipulation of annexin A11 and its associated genes may represent a novel therapeutic strategy in human ovarian cancers...
  97. pmc Expression of Fatty Acid Synthase Depends on NAC1 and Is Associated with Recurrent Ovarian Serous Carcinomas
    Stefanie M Ueda
    Department of Gynecology and Obstetrics, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    J Oncol 2010:285191. 2010
    ..The dependence of drug resistant tumor cells on FASN suggests a potential application of FASN-based therapeutics for recurrent ovarian cancer patients...
  98. pmc Digital karyotyping identifies thymidylate synthase amplification as a mechanism of resistance to 5-fluorouracil in metastatic colorectal cancer patients
    Tian Li Wang
    The Howard Hughes Medical Institute, Sidney Kimmel Comprehensive Cancer Center and Department of Surgery, The Johns Hopkins University Medical Institutions, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 101:3089-94. 2004
    ..01). These data suggest that genetic amplification of TYMS is a major mechanism of 5-FU resistance in vivo and have important implications for the management of colorectal cancer patients with recurrent disease...
  99. pmc Identification of Pbx1, a potential oncogene, as a Notch3 target gene in ovarian cancer
    Joon T Park
    Department of Pathology, Pathobiology Graduate Program, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 68:8852-60. 2008
    ..Taken together, the above findings indicate that Pbx1 is a direct Notch3-regulated gene that mediates the survival signal of Notch3 in ovarian cancer...
  100. pmc Ovarian tumorigenesis: a proposed model based on morphological and molecular genetic analysis
    Ie Ming Shih
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Pathol 164:1511-8. 2004
    ..This model of carcinogenesis reconciles the relationship of borderline tumors to invasive carcinoma and provides a morphological and molecular framework for studies aimed at elucidating the pathogenesis of ovarian cancer...
  101. pmc Ovarian low-grade and high-grade serous carcinoma: pathogenesis, clinicopathologic and molecular biologic features, and diagnostic problems
    Russell Vang
    Department of Pathology Division of Gynecologic Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Adv Anat Pathol 16:267-82. 2009
    ..This review provides an update on the pathogenesis and clinicopathologic features of these 2 types of serous carcinomas and addresses some of the diagnostic problems that are encountered in routine practice...

Research Grants11

  1. The Roles of HBXAP Gene in Ovarian Cancer
    Ie Ming Shih; Fiscal Year: 2010
    ..Revealing the molecular context in deciphering the functions of a tumor-promoting gene is essential to understand the pathogenesis of cancer development and may have translational implications for new cancer therapy. ..
  2. Molecular Diagnostics for Malignant Effusion
    Ie Ming Shih; Fiscal Year: 2010
    ..It is expected that the results from this study will elucidate the roles of NAC-1 in tumor progression and will provide the molecular foundation for future development of NAC-1 targeted therapy in recurrent cancers. ..
  3. Molecular Diagnostics for Malignant Effusion
    Ie Ming Shih; Fiscal Year: 2006
    ..The results from this proposal will likely introduce new paradigms to molecular cytology and provide oncologists a new perspective in managing their patients. ..
  4. Development of Digital SNP Analysis in Cancer Detection
    Ie Ming Shih; Fiscal Year: 2005
    ..Thus, the encouraging results from this study will ensure the detection of AI in plasma a promising cancer research direction for further exploration. ..
  5. Molecular Diagnostics for Malignant Effusion
    Ie Ming Shih; Fiscal Year: 2011
    ..It is expected that the results from this study will elucidate the roles of NAC-1 in tumor progression and will provide the molecular foundation for future development of NAC-1 targeted therapy in recurrent cancers. ..