RONALD SCHNAAR

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides
    Ronald L Schnaar
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Anal Biochem 302:276-84. 2002
  2. ncbi request reprint A "glyconutrient sham"
    Ronald L Schnaar
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Glycobiology 18:652-7; discussion 658-63. 2008
  3. pmc Brain gangliosides in axon-myelin stability and axon regeneration
    Ronald L Schnaar
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    FEBS Lett 584:1741-7. 2010
  4. pmc Myelin-associated glycoprotein and its axonal receptors
    Ronald L Schnaar
    Department of Pharmacology, The Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    J Neurosci Res 87:3267-76. 2009
  5. ncbi request reprint Glycolipid-mediated cell-cell recognition in inflammation and nerve regeneration
    Ronald L Schnaar
    Departments of Pharmacology and Neuroscience, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Arch Biochem Biophys 426:163-72. 2004
  6. pmc Gangliosides are functional nerve cell ligands for myelin-associated glycoprotein (MAG), an inhibitor of nerve regeneration
    Alka A Vyas
    Departments of Pharmacology and Neuroscience, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:8412-7. 2002
  7. doi request reprint Structural requirements of anti-GD1a antibodies determine their target specificity
    Pablo H H Lopez
    Department of Neurology, Johns Hopkins Hospital, Baltimore, MD 21287, USA
    Brain 131:1926-39. 2008
  8. pmc Axonal protective effects of the myelin-associated glycoprotein
    Thien Nguyen
    Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    J Neurosci 29:630-7. 2009
  9. pmc Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow
    Susan N Thomas
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 283:15647-55. 2008
  10. pmc E-selectin receptors on human leukocytes
    Leonardo Nimrichter
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Blood 112:3744-52. 2008

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides
    Ronald L Schnaar
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Anal Biochem 302:276-84. 2002
    ..These mAbs may provide useful tools for delineation of the expression and function of the major brain gangliosides and for probing the pathology of anti-ganglioside autoimmune diseases...
  2. ncbi request reprint A "glyconutrient sham"
    Ronald L Schnaar
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Glycobiology 18:652-7; discussion 658-63. 2008
    ..Here we address the relationship between glyconutrients and glycobiology, and how glyconutrient claims may impact the public and our discipline...
  3. pmc Brain gangliosides in axon-myelin stability and axon regeneration
    Ronald L Schnaar
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    FEBS Lett 584:1741-7. 2010
    ..Knowledge of the molecular interactions of brain gangliosides may improve understanding of axon-myelin stability and provide opportunities to enhance recovery after nerve injury...
  4. pmc Myelin-associated glycoprotein and its axonal receptors
    Ronald L Schnaar
    Department of Pharmacology, The Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    J Neurosci Res 87:3267-76. 2009
    ..Controversies remain about which receptor(s) mediates which of MAG's biological effects. Here we review the findings and challenges in associating MAG's biological effects with specific receptors...
  5. ncbi request reprint Glycolipid-mediated cell-cell recognition in inflammation and nerve regeneration
    Ronald L Schnaar
    Departments of Pharmacology and Neuroscience, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Arch Biochem Biophys 426:163-72. 2004
    ....
  6. pmc Gangliosides are functional nerve cell ligands for myelin-associated glycoprotein (MAG), an inhibitor of nerve regeneration
    Alka A Vyas
    Departments of Pharmacology and Neuroscience, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:8412-7. 2002
    ..These data implicate the nerve cell surface gangliosides GD1a and GT1b as functional MAG ligands and suggest that the first step in MAG inhibition is multivalent ganglioside clustering...
  7. doi request reprint Structural requirements of anti-GD1a antibodies determine their target specificity
    Pablo H H Lopez
    Department of Neurology, Johns Hopkins Hospital, Baltimore, MD 21287, USA
    Brain 131:1926-39. 2008
    ....
  8. pmc Axonal protective effects of the myelin-associated glycoprotein
    Thien Nguyen
    Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    J Neurosci 29:630-7. 2009
    ..Exploiting this pathway may lead to therapeutic strategies for neurological diseases characterized by axonal loss...
  9. pmc Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow
    Susan N Thomas
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 283:15647-55. 2008
    ..The novel finding that CEA is an E- and L-selectin ligand may explain the enhanced metastatic potential associated with tumor cell CEA overexpression and the supportive role of selectins in metastasis...
  10. pmc E-selectin receptors on human leukocytes
    Leonardo Nimrichter
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Blood 112:3744-52. 2008
    ....
  11. pmc Gangliosides and Nogo receptors independently mediate myelin-associated glycoprotein inhibition of neurite outgrowth in different nerve cells
    Niraj R Mehta
    Department of Pharmacology, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 282:27875-86. 2007
    ..Our data indicate that MAG inhibits axon outgrowth via two independent receptors, gangliosides and NgRs...
  12. pmc Myelin-associated glycoprotein and complementary axonal ligands, gangliosides, mediate axon stability in the CNS and PNS: neuropathology and behavioral deficits in single- and double-null mice
    Baohan Pan
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Exp Neurol 195:208-17. 2005
    ..Similar neuropathological and behavioral deficits in Galgt1-, Mag-, and double-null mice support the hypothesis that MAG binding to gangliosides contributes to long-term axon-myelin stability...
  13. pmc Potent glycan inhibitors of myelin-associated glycoprotein enhance axon outgrowth in vitro
    Alka A Vyas
    Department of Pharmacology, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:16305-10. 2005
    ..The ability to reverse MAG inhibition with monovalent glycosides encourages further exploration of glycans and glycan mimetics as blockers of MAG-mediated axon outgrowth inhibition...
  14. pmc Podocalyxin-like protein is an E-/L-selectin ligand on colon carcinoma cells: comparative biochemical properties of selectin ligands in host and tumor cells
    Susan N Thomas
    Dept of Chemical and Biomolecular Engineering, The Johns Hopkins Univ, 3400 N Charles St, Baltimore, MD 21218, USA
    Am J Physiol Cell Physiol 296:C505-13. 2009
    ..The novel finding that PCLP is an E-/L-selectin ligand on carcinoma cells offers a unifying perspective on the apparent enhanced metastatic potential associated with tumor cell PCLP overexpression and the role of selectins in metastasis...
  15. ncbi request reprint An anti-ganglioside antibody-secreting hybridoma induces neuropathy in mice
    Kazim A Sheikh
    Department of Neurology, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    Ann Neurol 56:228-39. 2004
    ..Our findings suggest that in addition to circulating antibodies, factors such as antibody accessibility and nerve fiber resistance to antibody-mediated injury play a role in the development of neuropathy...
  16. pmc Eosinophil-selective binding and proapoptotic effect in vitro of a synthetic Siglec-8 ligand, polymeric 6'-sulfated sialyl Lewis x
    Sherry A Hudson
    Division of Allergy and Clinical Immunology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Pharmacol Exp Ther 330:608-12. 2009
    ..These data demonstrate that a soluble, multivalent glycan selectively binds to human eosinophils and induces their apoptosis in vitro and provide proof-of-concept that such a reagent could be used to selectively target eosinophils...
  17. pmc Gangliosides in cell recognition and membrane protein regulation
    Pablo H H Lopez
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Curr Opin Struct Biol 19:549-57. 2009
    ..In these ways, gangliosides act as regulatory elements in the immune system, in the nervous system, in metabolic regulation, and in cancer progression...
  18. pmc Sialidase enhances recovery from spinal cord contusion injury
    Andrea Mountney
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:11561-6. 2010
    ..These findings validate sialoglycans as therapeutic targets and sialidase as a candidate therapy for spinal cord injury...
  19. pmc Passive transfer of IgG anti-GM1 antibodies impairs peripheral nerve repair
    Pablo H Lopez
    Department of Neurology, The Johns Hopkins University, Baltimore, Maryland 21287, USA
    J Neurosci 30:9533-41. 2010
    ..These data indicate that circulating immune effectors such as human autoantibodies, which are exogenous to the nervous system, can modulate axon regeneration/nerve repair in autoimmune neurological disorders such as GBS...
  20. ncbi request reprint Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells
    William D Hanley
    Department of The Johns Hopkins University, Baltimore, Maryland, USA
    FASEB J 20:337-9. 2006
    ..The novel finding that CD44v are selectin ligands offers a unifying perspective on the apparent enhanced metastatic potential associated with tumor cell CD44v overexpression and the critical role of selectins in metastasis...
  21. ncbi request reprint Oligomers of glycamino acid
    Yoshitomo Suhara
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725North Wolfe Street, Baltimore, MD 21205, USA
    Bioorg Med Chem 10:1999-2013. 2002
    ....
  22. pmc Determination of glycolipid-protein interaction specificity
    Pablo H H Lopez
    Department of Neurology, John Hopkins University School of Medicine, Baltimore, Maryland, USA
    Methods Enzymol 417:205-20. 2006
    ..Three particularly useful methods to probe specific glycolipid-mediated recognition events are microwell adsorption (ELISA), thin layer chromatography overlay, and surface plasmon resonance (SPR) spectroscopy...
  23. ncbi request reprint Myelin-associated glycoprotein (Siglec-4) expression is progressively and selectively decreased in the brains of mice lacking complex gangliosides
    Ji Sun
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Glycobiology 14:851-7. 2004
    ..We conclude that the maintenance of MAG protein levels depends on the presence of complex gangliosides, perhaps due to enhanced stability when MAG on myelin binds to its complementary ligands, GD1a and GT1b, on the apposing axon surface...
  24. pmc Sialidase enhances spinal axon outgrowth in vivo
    Lynda J S Yang
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 103:11057-62. 2006
    ..Molecular therapies targeting sialoglycoconjugates and CSPGs may aid functional recovery after brachial plexus avulsion or other nervous system injuries and diseases...
  25. ncbi request reprint Glycan array screening reveals a candidate ligand for Siglec-8
    Bruce S Bochner
    Division of Allergy and Clinical Immunology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Biol Chem 280:4307-12. 2005
    ..We conclude that Siglec-8 binds preferentially to the sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl...
  26. pmc Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers
    Sheng Ce Tao
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University, Baltimore, MD 21205, USA
    Glycobiology 18:761-9. 2008
    ..Thus, lectin microarrays are an effective tool for analyzing diverse cell processes including cell development and differentiation, cell-cell communication, pathogen-host recognition, and cell surface biomarker identification...
  27. ncbi request reprint UDP-glucuronate decarboxylase, a key enzyme in proteoglycan synthesis: cloning, characterization, and localization
    John L Moriarity
    Department of Neurological Surgery, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 277:16968-75. 2002
    ..Subcellular studies and histochemistry localized UGD protein to the perinuclear Golgi where xylosylation of proteoglycan core proteins is known to occur...
  28. ncbi request reprint Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids
    Yumi Tagawa
    Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Neurochem Res 27:847-55. 2002
    ..These data support the hypothesis that gangliosides bearing very long chain fatty acids are differentially displayed on membranes, which may lead to altered antigenicity...
  29. ncbi request reprint Intact cell adhesion to glycan microarrays
    Leonardo Nimrichter
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Glycobiology 14:197-203. 2004
    ..The described method is a robust approach to quantify selective cell adhesion using a wide variety of glycans and may contribute to the repertoire of tools for the study of glycomics...
  30. ncbi request reprint Systematic synthesis and MAG-binding activity of novel sulfated GM1b analogues as mimics of Chol-1 (alpha-series) gangliosides: highly active ligands for neural siglecs
    Hiromi Ito
    Department of Applied Bio organic Chemistry, Gifu University, Gifu 501 1193, Japan
    Carbohydr Res 338:1621-39. 2003
    ..Among the synthetic gangliosides, GSC-338 (II3III6-disulfate of iso-GM1b) was surprisingly found to be the most potent MAG binding structure tested to date...
  31. ncbi request reprint Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation
    Marija Heffer-Lauc
    Department of Biology, University of Osijek School of Medicine, J Huttlera 4, 31000 Osijek, Croatia
    Biochim Biophys Acta 1686:200-8. 2005
    ..These data raise technical challenges for using nonionic detergents in certain histological protocols and for isolation of lipid rafts from brain tissue...
  32. pmc Synthesis of reference standards to enable single cell metabolomic studies of tetramethylrhodamine-labeled ganglioside GM1
    E Andreas Larsson
    Carlsberg Laboratory, Valby Copenhagen DK 2500, Denmark
    Carbohydr Res 342:482-9. 2007
    ..All eight compounds could be separated within 4 min in capillary electrophoresis where they could be detected at the zeptomole (ca. 1000 molecule) level using LIF...
  33. pmc Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections
    Marija Heffer-Lauc
    Department of Medical Biology, University of Osijek School of Medicine, J Huttlera 4, 31000, Osijek, Croatia
    J Histochem Cytochem 55:805-12. 2007
    ..This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials...
  34. ncbi request reprint Metabolic cytometry. Glycosphingolipid metabolism in single cells
    Colin D Whitmore
    Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA
    Anal Chem 79:5139-42. 2007
    ..The average cell took up roughly 2 amol (10(6) copies) of the labeled substrate. This method allows determination of cell-to-cell diversity and regulation of glycosphingolipid metabolism...

Research Grants16

  1. Targeting Endogenous Inhibitors to Enhance Spinal Axon Regeneration After Injury
    Ronald L Schnaar; Fiscal Year: 2010
    ..Destroying or blocking these molecules may permit axons to regenerate, greatly enhancing functional recovery. ..
  2. Targeting Endogenous Inhibitors to Enhance Spinal Axon Regeneration After Injury
    RONALD SCHNAAR; Fiscal Year: 2009
    ..Destroying or blocking these molecules may permit axons to regenerate, greatly enhancing functional recovery. ..
  3. COMPLEX CARBOHYDRATES IN NEURONAL CELL FUNCTION
    RONALD SCHNAAR; Fiscal Year: 2007
    ..We will use cell surface ganglioside engineering in situ to identify ganglioside-associated proteins that may serve as proximal molecules in ganglioside-mediated signaling. ..
  4. Enhancing axon regeneration by multi-inhibitor blocking
    RONALD SCHNAAR; Fiscal Year: 2005
    ..The resulting data may provide insight on the relative contributions of the different ARI's in an in vivo model, and may support evaluation of new approaches to enhance axon regeneration after CNS injury. ..
  5. COMPLEX CARBOHYDRATES IN NEURONAL CELL FUNCTION
    RONALD SCHNAAR; Fiscal Year: 2004
    ....
  6. Targeting Endogenous Inhibitors to Enhance Spinal Axon Regeneration After Injury
    RONALD SCHNAAR; Fiscal Year: 2009
    ..Destroying or blocking these molecules may permit axons to regenerate, greatly enhancing functional recovery. ..