Douglas Robinson

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Cortical mechanics and meiosis II completion in mammalian oocytes are mediated by myosin-II and Ezrin-Radixin-Moesin (ERM) proteins
    Stephanie M Larson
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Biol Cell 21:3182-92. 2010
  2. pmc Bringing the physical sciences into your cell biology research
    Douglas N Robinson
    Department of Cell Biology and Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 23:4167-70. 2012
  3. pmc Automated characterization of cell shape changes during amoeboid motility by skeletonization
    Yuan Xiong
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Syst Biol 4:33. 2010
  4. pmc Modeling cellular deformations using the level set formalism
    Liu Yang
    Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Syst Biol 2:68. 2008
  5. pmc Dynacortin facilitates polarization of chemotaxing cells
    Cathryn Kabacoff
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    BMC Biol 5:53. 2007
  6. ncbi request reprint Dictyostelium cytokinesis: from molecules to mechanics
    Douglas N Robinson
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    J Muscle Res Cell Motil 23:719-27. 2002
  7. pmc Dynacortin contributes to cortical viscoelasticity and helps define the shape changes of cytokinesis
    Kristine D Girard
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    EMBO J 23:1536-46. 2004
  8. pmc Mitosis-specific mechanosensing and contractile-protein redistribution control cell shape
    Janet C Effler
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, Maryland 21205, USA
    Curr Biol 16:1962-7. 2006
  9. pmc Interactions between myosin and actin crosslinkers control cytokinesis contractility dynamics and mechanics
    Elizabeth M Reichl
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Curr Biol 18:471-80. 2008
  10. ncbi request reprint Mechanics and regulation of cytokinesis
    Douglas N Robinson
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Curr Opin Cell Biol 16:182-8. 2004

Research Grants

Collaborators

  • S C Kuo
  • Cathryn Kabacoff
  • Janice P Evans
  • Pablo A Iglesias
  • RICHARD FIRTEL
  • Liu Yang
  • Janet C Effler
  • Elizabeth M Reichl
  • Kristine D Girard
  • Yixin Ren
  • Yee Seir Kee
  • Stephanie M Larson
  • Yuan Xiong
  • Edelyn Octtaviani
  • Michael Delannoy
  • Wendy Zhang
  • Lauren M Matthews
  • Peter N Devreotes
  • Hyo J Lee
  • Pei Hsuan Hung
  • Jonathan Franca-Koh
  • Tianzhi Luo
  • Ronald S Rock
  • Melanie Norstrom
  • Mary K Morphew
  • Srikanth Divi
  • Jason M Berk
  • Minhchau N Tran
  • Charles Chaney

Detail Information

Publications20

  1. pmc Cortical mechanics and meiosis II completion in mammalian oocytes are mediated by myosin-II and Ezrin-Radixin-Moesin (ERM) proteins
    Stephanie M Larson
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Biol Cell 21:3182-92. 2010
    ..Thus, myosin-II and ERM proteins modulate mechanical properties in oocytes, contributing to cell polarity and to completion of meiosis...
  2. pmc Bringing the physical sciences into your cell biology research
    Douglas N Robinson
    Department of Cell Biology and Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 23:4167-70. 2012
    ..This effort requires an interdisciplinary approach by investigators from both the biological and the physical sciences...
  3. pmc Automated characterization of cell shape changes during amoeboid motility by skeletonization
    Yuan Xiong
    Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Syst Biol 4:33. 2010
    ....
  4. pmc Modeling cellular deformations using the level set formalism
    Liu Yang
    Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Syst Biol 2:68. 2008
    ..Here we demonstrate that accurate cell shape changes can be recreated using level set methods (LSM), in which the cellular shape is defined implicitly, thereby eschewing the need for updating boundaries...
  5. pmc Dynacortin facilitates polarization of chemotaxing cells
    Cathryn Kabacoff
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    BMC Biol 5:53. 2007
    ..Dynacortin also becomes highly enriched in cortical protrusions, which are sites of new actin assembly...
  6. ncbi request reprint Dictyostelium cytokinesis: from molecules to mechanics
    Douglas N Robinson
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    J Muscle Res Cell Motil 23:719-27. 2002
    ..Genetic, biochemical, and mechanical measurements are providing a quantitative view of how real proteins control this essential life process...
  7. pmc Dynacortin contributes to cortical viscoelasticity and helps define the shape changes of cytokinesis
    Kristine D Girard
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    EMBO J 23:1536-46. 2004
    ..The combination of genetic, biochemical, and biophysical observations suggests that the cell's cortical mechanical properties control how the cortex is remodeled during cytokinesis...
  8. pmc Mitosis-specific mechanosensing and contractile-protein redistribution control cell shape
    Janet C Effler
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, Maryland 21205, USA
    Curr Biol 16:1962-7. 2006
    ..Our data provide evidence for a mechanosensory system that directs contractile proteins to regulate cell shape during mitosis...
  9. pmc Interactions between myosin and actin crosslinkers control cytokinesis contractility dynamics and mechanics
    Elizabeth M Reichl
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Curr Biol 18:471-80. 2008
    ..Discerning how this integrated network operates is essential for understanding cytokinesis contractility and shape control. Here, we analyzed the cytoskeletal network that drives furrow ingression in Dictyostelium...
  10. ncbi request reprint Mechanics and regulation of cytokinesis
    Douglas N Robinson
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Curr Opin Cell Biol 16:182-8. 2004
    ..A genuine quantitative understanding of cytokinesis must include a thorough analysis of the mechanical properties of the cortex and how signals modify these properties to dictate a well-controlled, error-free cytokinesis...
  11. pmc Dictyostelium myosin II mechanochemistry promotes active behavior of the cortex on long time scales
    Kristine D Girard
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 103:2103-8. 2006
    ..Consistent with its low duty ratio, myosin II does not directly drive active bead motility. Instead, myosin II and dynacortin antagonistically regulate other active processes in the living cortex...
  12. doi request reprint Motor proteins: myosin mechanosensors
    Yee Seir Kee
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, Maryland 21205, USA
    Curr Biol 18:R860-2. 2008
    ..Mechanosensation is emerging as a general principle of myosin motors. As demonstrated in a recent study, the single-headed myosin I molecule is an exquisite mechanosensor, able to sense strain over a very small force range...
  13. pmc Mechanosensing through cooperative interactions between myosin II and the actin crosslinker cortexillin I
    Yixin Ren
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Curr Biol 19:1421-8. 2009
    ..For a myosin motor to experience tension, the actin filament must also be anchored...
  14. ncbi request reprint Putting the brakes on cytokinesis with alpha-actinin
    Elizabeth M Reichl
    Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 13:460-2. 2007
    ..In this issue of Developmental Cell, Mukhina et al. (2007) show that myosin-II modulates the cortical lifetime of the actin crosslinker alpha-actinin, which in turn tunes actin filament dynamics, thereby controlling furrow ingression...
  15. ncbi request reprint A mechanosensory system controls cell shape changes during mitosis
    Janet C Effler
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Cell Cycle 6:30-5. 2007
    ..In this review, we summarize these findings and their implications for cytokinesis regulation and for understanding the cytoskeletal system architecture that governs shape control...
  16. pmc Enlazin, a natural fusion of two classes of canonical cytoskeletal proteins, contributes to cytokinesis dynamics
    Edelyn Octtaviani
    Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 17:5275-86. 2006
    ..Thus, enlazin has roles in cell mechanics and adhesion, and these roles seem to be differentially significant for cytokinesis, depending on the genetic background...
  17. ncbi request reprint The stress and strain of cytokinesis
    Elizabeth M Reichl
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Trends Cell Biol 15:200-6. 2005
    ..Finally, we present our current understanding of how cytokinesis mechanics work...
  18. pmc Balance of actively generated contractile and resistive forces controls cytokinesis dynamics
    Wendy Zhang
    Department of Physics, James Franck Institute, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 102:7186-91. 2005
    ..Finally, we propose that, in addition to myosin II, a Laplace pressure, resulting from material properties and the geometry of the dividing cell, generates force to help drive furrow ingression late in cytokinesis...
  19. pmc Quantitation of the distribution and flux of myosin-II during cytokinesis
    Douglas N Robinson
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    BMC Cell Biol 3:4. 2002
    ..To achieve this goal, the distribution and flux of the cell division machinery must be quantified. Here we report the first quantitative analysis of the distribution and flux of myosin-II, an essential element of the contractile ring...
  20. ncbi request reprint Dynacortin is a novel actin bundling protein that localizes to dynamic actin structures
    Douglas N Robinson
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305 5307, USA
    J Biol Chem 277:9088-95. 2002
    ..These results indicate that cells have evolved different actin-filament cross-linking proteins with complementary cellular distributions that collaborate to orchestrate complex cell shape changes...

Research Grants9

  1. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas Robinson; Fiscal Year: 2003
    ..Thus, this molecule is of considerable interest because of its role in cortical function and cell shape control and its unusual domain structure. ..
  2. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas Robinson; Fiscal Year: 2004
    ..Thus, this molecule is of considerable interest because of its role in cortical function and cell shape control and its unusual domain structure. ..
  3. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas Robinson; Fiscal Year: 2004
    ..Finally, new genes involved in cortical shape control will be identified by saturating the genetic selection experiment for suppressors of cortexillin I and by screening for enhancers of myosin-II null cells. ..
  4. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas Robinson; Fiscal Year: 2005
    ..Thus, this molecule is of considerable interest because of its role in cortical function and cell shape control and its unusual domain structure. ..
  5. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas Robinson; Fiscal Year: 2006
    ..Thus, this molecule is of considerable interest because of its role in cortical function and cell shape control and its unusual domain structure. ..
  6. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas Robinson; Fiscal Year: 2007
    ..Thus, this molecule is of considerable interest because of its role in cortical function and cell shape control and its unusual domain structure. ..
  7. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas Robinson; Fiscal Year: 2009
    ..Ultimately, with a rigorous understanding and a complete molecular handle on the process, it should be possible to develop better cancer therapies that are tailored to the properties of specific types of cancer cells. ..
  8. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas N Robinson; Fiscal Year: 2010
    ..Ultimately, with a rigorous understanding and a complete molecular handle on the process, it should be possible to develop better cancer therapies that are tailored to the properties of specific types of cancer cells. ..
  9. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas N Robinson; Fiscal Year: 2010
    ..Ultimately, with a rigorous understanding and a complete molecular handle on the process, it should be possible to develop better cancer therapies that are tailored to the properties of specific types of cancer cells. ..