Feng Qian

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Th1/Th2 CD4+ T cell responses against NY-ESO-1 in HLA-DPB1*0401/0402 patients with epithelial ovarian cancer
    Feng Qian
    Department of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Cancer Immun 4:12. 2004
  2. pmc Prestin modulates mechanics and electromechanical force of the plasma membrane
    Rui Zhang
    Applied Physics Graduate Program, Rice University, Houston, Texas, USA
    Biophys J 93:L07-9. 2007
  3. pmc Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure
    Shengqiang Yu
    Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:18688-93. 2007
  4. ncbi request reprint Cellular and molecular function of mucolipins (TRPML) and polycystin 2 (TRPP2)
    Feng Qian
    Division of Nephrology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
    Pflugers Arch 451:277-85. 2005
  5. pmc Polycystin-1 interacts with inositol 1,4,5-trisphosphate receptor to modulate intracellular Ca2+ signaling with implications for polycystic kidney disease
    Yun Li
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:36431-41. 2009
  6. ncbi request reprint Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene
    Wen Wei
    Division of Nephrology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 282:21729-37. 2007
  7. ncbi request reprint A regulatory role of polycystin-1 on cystic fibrosis transmembrane conductance regulator plasma membrane expression
    Masahiro Ikeda
    Department of Physiology, Johns Hopkins University School of Medicine 725 N olfe St, Baltimore, MD 21205, USA
    Cell Physiol Biochem 18:9-20. 2006
  8. pmc Cleavage of polycystin-1 requires the receptor for egg jelly domain and is disrupted by human autosomal-dominant polycystic kidney disease 1-associated mutations
    Feng Qian
    Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:16981-6. 2002
  9. ncbi request reprint A functional floxed allele of Pkd1 that can be conditionally inactivated in vivo
    Klaus B Piontek
    Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    J Am Soc Nephrol 15:3035-43. 2004
  10. pmc Polycystin signaling is required for directed endothelial cell migration and lymphatic development
    Patricia Outeda
    Division of Nephrology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Cell Rep 7:634-44. 2014

Research Grants

  1. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2007
  2. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2010
  3. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2004
  4. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2003
  5. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2004
  6. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2005
  7. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2004
  8. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2005
  9. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2006
  10. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2006

Collaborators

Detail Information

Publications35

  1. ncbi request reprint Th1/Th2 CD4+ T cell responses against NY-ESO-1 in HLA-DPB1*0401/0402 patients with epithelial ovarian cancer
    Feng Qian
    Department of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Cancer Immun 4:12. 2004
    ..Our study supports the relevance of cancer vaccine trials with the NY-ESO-1 epitope 157-170 in HLA-DP4+ EOC patients with NY-ESO-1-expressing tumors and strategies to improve Th1-dominated tumor-reactive CD4+ T cell bias...
  2. pmc Prestin modulates mechanics and electromechanical force of the plasma membrane
    Rui Zhang
    Applied Physics Graduate Program, Rice University, Houston, Texas, USA
    Biophys J 93:L07-9. 2007
    ..These results suggest that prestin-associated charge transfer is necessary for maximal EMF generation by the membrane...
  3. pmc Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure
    Shengqiang Yu
    Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:18688-93. 2007
    ..Our study establishes a critical but restricted role of cleavage for PC1 function and suggests a differential function of the two types of PC1 molecules in vivo...
  4. ncbi request reprint Cellular and molecular function of mucolipins (TRPML) and polycystin 2 (TRPP2)
    Feng Qian
    Division of Nephrology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
    Pflugers Arch 451:277-85. 2005
    ..Here we review the function of TRPML1 and TRPP2 as representative members of these families, focusing on the genetics, physiology, and biochemistry...
  5. pmc Polycystin-1 interacts with inositol 1,4,5-trisphosphate receptor to modulate intracellular Ca2+ signaling with implications for polycystic kidney disease
    Yun Li
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:36431-41. 2009
    ..These data demonstrate that PC1 inhibits Ca(2+) release, perhaps opposing the effect of PC2, which facilitates Ca(2+) release through the IP(3)R...
  6. ncbi request reprint Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene
    Wen Wei
    Division of Nephrology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 282:21729-37. 2007
    ..We provide evidence that the cleavage occurs via a cis-autoproteolytic mechanism involving an ester intermediate as shown for Ntn hydrolases and EMR2...
  7. ncbi request reprint A regulatory role of polycystin-1 on cystic fibrosis transmembrane conductance regulator plasma membrane expression
    Masahiro Ikeda
    Department of Physiology, Johns Hopkins University School of Medicine 725 N olfe St, Baltimore, MD 21205, USA
    Cell Physiol Biochem 18:9-20. 2006
    ..Moreover, these findings suggest that the malfunction of PC-1 enhances plasma membrane expression of CFTR, thus causing abnormal Cl(-)secretion into the cyst lumen...
  8. pmc Cleavage of polycystin-1 requires the receptor for egg jelly domain and is disrupted by human autosomal-dominant polycystic kidney disease 1-associated mutations
    Feng Qian
    Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:16981-6. 2002
    ..We conclude that the cleavage of polycystin-1 is likely essential for its biologic activity...
  9. ncbi request reprint A functional floxed allele of Pkd1 that can be conditionally inactivated in vivo
    Klaus B Piontek
    Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    J Am Soc Nephrol 15:3035-43. 2004
    ..This new line of mice will be invaluable in the study of Pkd1 biology and serve as a powerful new tool that can be used to study the pathogenesis of autosomal dominant polycystic kidney disease...
  10. pmc Polycystin signaling is required for directed endothelial cell migration and lymphatic development
    Patricia Outeda
    Division of Nephrology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Cell Rep 7:634-44. 2014
    ..Our studies reveal a role for polycystin signaling in lymphatic development. ..
  11. ncbi request reprint Polycystin 2 interacts with type I inositol 1,4,5-trisphosphate receptor to modulate intracellular Ca2+ signaling
    Yun Li
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:41298-306. 2005
    ..Therefore, mutations in either PC1 or PC2 could result in the misregulation of intracellular Ca2+ signaling, which in turn could contribute to the pathology of autosomal dominant polycystic kidney disease...
  12. pmc G-protein signaling modulator 1 deficiency accelerates cystic disease in an orthologous mouse model of autosomal dominant polycystic kidney disease
    Michelle Kwon
    Division of Nephrology, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Proc Natl Acad Sci U S A 109:21462-7. 2012
    ....
  13. pmc A Pkd1-Fbn1 genetic interaction implicates TGF-β signaling in the pathogenesis of vascular complications in autosomal dominant polycystic kidney disease
    Dongyan Liu
    Division of Nephrology, University of Maryland School of Medicine, Baltimore, Maryland
    J Am Soc Nephrol 25:81-91. 2014
    ..In addition, we demonstrate that loss of PKD1 alone is sufficient to induce a heightened responsiveness to TGF-β. Our data link the interaction of two important diseases to a fundamental signaling pathway. ..
  14. pmc The nanomechanics of polycystin-1 extracellular region
    Feng Qian
    Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:40723-30. 2005
    ..These force-driven reactions may be important for cell elasticity and the regulation of cell signaling events mediated by PC1...
  15. pmc Evaluating the clinical utility of a molecular genetic test for polycystic kidney disease
    Miguel A Garcia-Gonzalez
    Johns Hopkins University School of Medicine, Department of Medicine, Division of Nephrology, Baltimore, MD 21205, USA, and Laboratorio de Investigación en Nefroloxía, Complexo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain
    Mol Genet Metab 92:160-7. 2007
    ..We conclude that a significant fraction of ADPKD mutations are caused by amino acid substitutions that need to be interpreted carefully when utilized in clinical decision-making...
  16. ncbi request reprint PKD1 induces p21(waf1) and regulation of the cell cycle via direct activation of the JAK-STAT signaling pathway in a process requiring PKD2
    Anil Kumar Bhunia
    Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 109:157-68. 2002
    ..These results suggest that one function of the polycystin-1/2 complex is to regulate the JAK/STAT pathway and explain how mutations of either gene can result in dysregulated growth...
  17. ncbi request reprint [Views on sequence variations of AMA-1 in Plasmodium falciparum FCC1/HN isolate]
    Feng Qian
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22:256. 2004
  18. pmc Distinct functions for different scl isoforms in zebrafish primitive and definitive hematopoiesis
    Feng Qian
    Laboratory of Molecular and Developmental Immunology, Institute of Molecular and Cell Biology, Singapore, Singapore
    PLoS Biol 5:e132. 2007
    ..Our findings suggest that hematopoietic cells at different levels of hierarchy are likely governed by a gradient of the Scl protein established through temporal and spatial patterns of expression of the different isoforms...
  19. ncbi request reprint pPIC9-Fc: a vector system for the production of single-chain Fv-Fc fusions in Pichia pastoris as detection reagents in vitro
    Jianglan Liu
    State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China
    J Biochem 134:911-7. 2003
    ....
  20. ncbi request reprint NY-ESO-1 and LAGE-1 cancer-testis antigens are potential targets for immunotherapy in epithelial ovarian cancer
    Kunle Odunsi
    Division of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    Cancer Res 63:6076-83. 2003
    ..These findings indicate that NY-ESO-1 and LAGE-1 are attractive targets for antigen-specific immunotherapy in EOC...
  21. ncbi request reprint Effects of prestin on membrane mechanics and electromechanics
    Bahman Anvari
    Department of Bioengineering, University of California, Riverside, CA 92521, USA
    Conf Proc IEEE Eng Med Biol Soc 2007:5384-6. 2007
    ..We propose that prestin and membrane work in synergy to produce the electrical and mechanical changes that are required during OHC electromotility...
  22. ncbi request reprint [Determination of free thiols in the chimeric protein PfCP-2.9 of Plasmodium falciparum]
    Feng Qian
    Department of Etiological Biology, Second Military Medical University, Shanghai 200433
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 21:99-101. 2003
    ..To determine the free thiols in the chimeric protein PfCP-2.9 of Plasmodium falciparum expressed by Pichia pastoris...
  23. pmc Addition of CpG ODN to recombinant Pseudomonas aeruginosa ExoProtein A conjugates of AMA1 and Pfs25 greatly increases the number of responders
    Feng Qian
    Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    Vaccine 26:2521-7. 2008
    ..The results obtained in this study indicate the potential use of a combination strategy to increase the number of responders to malarial antigens in humans...
  24. ncbi request reprint [Enhancement by an in vivo-activated promoter of immunogenicity of recombinant attenuated Salmonella typhimurium expressing hepatitis C virus core antigen]
    Ping Zhao
    Department of Microbiology, Second Military Medical University, Shanghai 200433, China
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:266-70. 2003
    ....
  25. pmc Expression of PKD1 and PKD2 transcripts and proteins in human embryo and during normal kidney development
    Veronique Chauvet
    INSERM U423 and the Département deGénétique et Unité INSERM U393, Hopital Necker Enfants Malades, Paris, France
    Am J Pathol 160:973-83. 2002
    ..These data suggest that polycystins could interact with different partners, at least during kidney development...
  26. pmc Conjugating recombinant proteins to Pseudomonas aeruginosa ExoProtein A: a strategy for enhancing immunogenicity of malaria vaccine candidates
    Feng Qian
    Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Rockville, MD 20852, USA
    Vaccine 25:3923-33. 2007
    ..These conjugates now need to be tested in humans to determine if mice are predictive of the response in humans...
  27. ncbi request reprint In vivo antitumor activity by 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone in a solid human carcinoma xenograft model
    Chun Lin Ye
    State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Cancer Chemother Pharmacol 56:70-4. 2005
    ..60+/-0.80% of the total in mice injected with 150 mg/kg DMC). To our knowledge, this is the first time that chalcone compounds have been applied to a human tumor xenograft model...
  28. ncbi request reprint A splice form of polycystin-2, lacking exon 7, does not interact with polycystin-1
    Karl Hackmann
    Institut fur Humangenetik, Universitatsklinikum Munster, Germany
    Hum Mol Genet 14:3249-62. 2005
    ..The predominant expression in brain indicates a function in this organ. The inability to interact with polycystin-1 expands further the PKD1-independent functions of polycystin-2 forms...
  29. ncbi request reprint Identification of differentially expressed genes in clinically distinct groups of serous ovarian carcinomas using cDNA microarray
    Yvonne Collins
    Department of Gynecologic Oncology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14261, USA
    Int J Mol Med 14:43-53. 2004
    ..Our study demonstrates differential gene expression in clinically distinct groups of SEOC using cDNA microarray. These genes may potentially be useful as biomarkers and/or targets for therapeutic intervention...
  30. ncbi request reprint Fusion of two malaria vaccine candidate antigens enhances product yield, immunogenicity, and antibody-mediated inhibition of parasite growth in vitro
    Weiqing Pan
    Department of Etiologic Biology, Second Military Medical University, Shanghai, China
    J Immunol 172:6167-74. 2004
    ..9. The combination of the extremely high yield of the protein and enhancement of its immune response provides a basis to develop an effective and affordable malaria vaccine...
  31. ncbi request reprint Studies of plasma membrane mechanics and plasma membrane-cytoskeleton interactions using optical tweezers and fluorescence imaging
    Sergey A Ermilov
    Department of Bioengineering, Rice University, P O Box 1892, MS 142, Houston, TX 77251 1892, USA
    J Biomech 40:476-80. 2007
    ....
  32. ncbi request reprint Influence of CD4+CD25+ regulatory T cells on low/high-avidity CD4+ T cells following peptide vaccination
    Hiroyoshi Nishikawa
    Ludwig Institute for Cancer Research, New York Branch, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    J Immunol 176:6340-6. 2006
    ..We propose that vaccination with NY-ESO-1(157-170) peptide recruits low-avidity T cells with low sensitivity to Tregs and fails to modulate the suppressive effect of Tregs on high-avidity NY-ESO-1-specific T cell precursors...
  33. ncbi request reprint [Relationship between efficacy of interferon-alpha and phenotypes of peripheral blood dendritic cells in patients with chronic hepatitis B]
    Chuan wu Zhu
    Department of Infectious Diseases, Fifth People s Hospital of Suzhou, Suzhou 215007, China
    Zhonghua Gan Zang Bing Za Zhi 12:174-5. 2004
  34. ncbi request reprint [Preparation and characterization of monoclonal antibody specific to PfCP-2.9 chimeric protein of Plasmodium falciparum]
    Rui Wang
    Department of Etiologic Biology, The Second Military Medical University, Shanghai, China
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 24:247-50. 2006
    ..To prepare and characterize monoclonal antibody against a malaria vaccine candidate, PfCP-2.9 chimeric protein of Plasmodium falciparum...
  35. ncbi request reprint TRP genes: candidates for nonselective cation channels and store-operated channels in insulin-secreting cells
    Feng Qian
    Department of Neurobiology, Physiology, and Pharmacology, The University of Chicago, Chicago, Illinois, USA
    Diabetes 51:S183-9. 2002
    ..TRPC-like channels may provide a pathway for depolarization or Ca(2+) entry in beta-cells and may be interesting targets for manipulating beta-cell function...

Research Grants35

  1. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2007
    ..The information from our studies will likely open new avenues in the research of ADPKD and establish the foundation for developing causative and effective therapies of the disease. ..
  2. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2010
    ..This study aims to understand Polycystin-1, the reasons it becomes defective, and the ways to prevent it. ..
  3. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2004
    ..The information from our studies will likely open new avenues in the research of ADPKD and establish the foundation for developing causative and effective therapies of the disease. ..
  4. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2003
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  5. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2004
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  6. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2005
    ..The information from our studies will likely open new avenues in the research of ADPKD and establish the foundation for developing causative and effective therapies of the disease. ..
  7. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2004
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  8. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2005
    ..abstract_text> ..
  9. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2006
    ..The information from our studies will likely open new avenues in the research of ADPKD and establish the foundation for developing causative and effective therapies of the disease. ..
  10. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2006
    ..abstract_text> ..
  11. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2007
    ..abstract_text> ..
  12. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2009
    ..This study aims to understand Polycystin-1, the reasons it becomes defective, and the ways to prevent it. ..
  13. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2003
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  14. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2003
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  15. NOVEL APPROACH TO THE MOLECULAR GENETICS OF PKD1
    Gregory Germino; Fiscal Year: 2003
    ..The latter will help define the pathophysiology of this disorder, allow the development and testing of therapeutic compounds and allow manipulation of the environmental and genetic background to assess the relative effects of each. ..
  16. NOVEL APPROACH TO THE MOLECULAR GENETICS OF PKD1
    Gregory Germino; Fiscal Year: 1999
    ..The latter will help define the pathophysiology of this disorder, allow the development and testing of therapeutic compounds and allow manipulation of the environmental and genetic background to assess the relative effects of each. ..
  17. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 1999
    ..The ultimate selection of a method will depend on the size of the gene and the rate of progress in the development of newer, more efficient technologies. ..
  18. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2000
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  19. NOVEL APPROACH TO THE MOLECULAR GENETICS OF PKD1
    Gregory Germino; Fiscal Year: 2000
    ..The latter will help define the pathophysiology of this disorder, allow the development and testing of therapeutic compounds and allow manipulation of the environmental and genetic background to assess the relative effects of each. ..
  20. NOVEL APPROACH TO THE MOLECULAR GENETICS OF PKD1
    Gregory Germino; Fiscal Year: 2001
    ..The latter will help define the pathophysiology of this disorder, allow the development and testing of therapeutic compounds and allow manipulation of the environmental and genetic background to assess the relative effects of each. ..
  21. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2001
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  22. NOVEL APPROACH TO THE MOLECULAR GENETICS OF PKD1
    Gregory Germino; Fiscal Year: 2002
    ..The latter will help define the pathophysiology of this disorder, allow the development and testing of therapeutic compounds and allow manipulation of the environmental and genetic background to assess the relative effects of each. ..
  23. MOLECULAR GENETICS OF HUMAN ARPKD
    Gregory Germino; Fiscal Year: 2002
    ..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
  24. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2003
    ..The information from our studies will likely open new avenues in the research of ADPKD and establish the foundation for developing causative and effective therapies of the disease. ..
  25. The Proteolytic Cleavage of Polycystin-1: How and Why
    Feng Qian; Fiscal Year: 2010
    ..This study aims to understand Polycystin-1, the reasons it becomes defective, and the ways to prevent it. ..