Research Topics
Genomes and Genes | Feng QianSummaryAffiliation: Johns Hopkins University Country: USA Publications
Research Grants
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Detail Information
Publications
Th1/Th2 CD4+ T cell responses against NY-ESO-1 in HLA-DPB1*0401/0402 patients with epithelial ovarian cancerFeng Qian
Department of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
Cancer Immun 4:12. 2004..Our study supports the relevance of cancer vaccine trials with the NY-ESO-1 epitope 157-170 in HLA-DP4+ EOC patients with NY-ESO-1-expressing tumors and strategies to improve Th1-dominated tumor-reactive CD4+ T cell bias...
Prestin modulates mechanics and electromechanical force of the plasma membraneRui Zhang
Applied Physics Graduate Program, Rice University, Houston, Texas, USA
Biophys J 93:L07-9. 2007..These results suggest that prestin-associated charge transfer is necessary for maximal EMF generation by the membrane...- Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structureShengqiang Yu
Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 104:18688-93. 2007..Our study establishes a critical but restricted role of cleavage for PC1 function and suggests a differential function of the two types of PC1 molecules in vivo... - Cellular and molecular function of mucolipins (TRPML) and polycystin 2 (TRPP2)Feng Qian
Division of Nephrology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
Pflugers Arch 451:277-85. 2005..Here we review the function of TRPML1 and TRPP2 as representative members of these families, focusing on the genetics, physiology, and biochemistry... - Polycystin-1 interacts with inositol 1,4,5-trisphosphate receptor to modulate intracellular Ca2+ signaling with implications for polycystic kidney diseaseYun Li
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 284:36431-41. 2009..These data demonstrate that PC1 inhibits Ca(2+) release, perhaps opposing the effect of PC2, which facilitates Ca(2+) release through the IP(3)R... - Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 geneWen Wei
Division of Nephrology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
J Biol Chem 282:21729-37. 2007..We provide evidence that the cleavage occurs via a cis-autoproteolytic mechanism involving an ester intermediate as shown for Ntn hydrolases and EMR2... - A regulatory role of polycystin-1 on cystic fibrosis transmembrane conductance regulator plasma membrane expressionMasahiro Ikeda
Department of Physiology, Johns Hopkins University School of Medicine 725 N olfe St, Baltimore, MD 21205, USA
Cell Physiol Biochem 18:9-20. 2006..Moreover, these findings suggest that the malfunction of PC-1 enhances plasma membrane expression of CFTR, thus causing abnormal Cl(-)secretion into the cyst lumen... - Cleavage of polycystin-1 requires the receptor for egg jelly domain and is disrupted by human autosomal-dominant polycystic kidney disease 1-associated mutationsFeng Qian
Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 99:16981-6. 2002..We conclude that the cleavage of polycystin-1 is likely essential for its biologic activity... - A functional floxed allele of Pkd1 that can be conditionally inactivated in vivoKlaus B Piontek
Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
J Am Soc Nephrol 15:3035-43. 2004..This new line of mice will be invaluable in the study of Pkd1 biology and serve as a powerful new tool that can be used to study the pathogenesis of autosomal dominant polycystic kidney disease... - Polycystin 2 interacts with type I inositol 1,4,5-trisphosphate receptor to modulate intracellular Ca2+ signalingYun Li
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 280:41298-306. 2005..Therefore, mutations in either PC1 or PC2 could result in the misregulation of intracellular Ca2+ signaling, which in turn could contribute to the pathology of autosomal dominant polycystic kidney disease... - Evaluating the clinical utility of a molecular genetic test for polycystic kidney diseaseMiguel A Garcia-Gonzalez
Johns Hopkins University School of Medicine, Department of Medicine, Division of Nephrology, Baltimore, MD 21205, USA, and Laboratorio de Investigación en Nefroloxía, Complexo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain
Mol Genet Metab 92:160-7. 2007..We conclude that a significant fraction of ADPKD mutations are caused by amino acid substitutions that need to be interpreted carefully when utilized in clinical decision-making... - The nanomechanics of polycystin-1 extracellular regionFeng Qian
Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 280:40723-30. 2005..These force-driven reactions may be important for cell elasticity and the regulation of cell signaling events mediated by PC1... - PKD1 induces p21(waf1) and regulation of the cell cycle via direct activation of the JAK-STAT signaling pathway in a process requiring PKD2Anil Kumar Bhunia
Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Cell 109:157-68. 2002..These results suggest that one function of the polycystin-1/2 complex is to regulate the JAK/STAT pathway and explain how mutations of either gene can result in dysregulated growth... - Studies of plasma membrane mechanics and plasma membrane-cytoskeleton interactions using optical tweezers and fluorescence imagingSergey A Ermilov
Department of Bioengineering, Rice University, P O Box 1892, MS 142, Houston, TX 77251 1892, USA
J Biomech 40:476-80. 2007.... - Addition of CpG ODN to recombinant Pseudomonas aeruginosa ExoProtein A conjugates of AMA1 and Pfs25 greatly increases the number of respondersFeng Qian
Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
Vaccine 26:2521-7. 2008..The results obtained in this study indicate the potential use of a combination strategy to increase the number of responders to malarial antigens in humans... - Influence of CD4+CD25+ regulatory T cells on low/high-avidity CD4+ T cells following peptide vaccinationHiroyoshi Nishikawa
Ludwig Institute for Cancer Research, New York Branch, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
J Immunol 176:6340-6. 2006..We propose that vaccination with NY-ESO-1(157-170) peptide recruits low-avidity T cells with low sensitivity to Tregs and fails to modulate the suppressive effect of Tregs on high-avidity NY-ESO-1-specific T cell precursors... - Distinct functions for different scl isoforms in zebrafish primitive and definitive hematopoiesisFeng Qian
Laboratory of Molecular and Developmental Immunology, Institute of Molecular and Cell Biology, Singapore, Singapore
PLoS Biol 5:e132. 2007..Our findings suggest that hematopoietic cells at different levels of hierarchy are likely governed by a gradient of the Scl protein established through temporal and spatial patterns of expression of the different isoforms... - Effects of prestin on membrane mechanics and electromechanicsBahman Anvari
Department of Bioengineering, University of California, Riverside, CA 92521, USA
Conf Proc IEEE Eng Med Biol Soc 2007:5384-6. 2007..We propose that prestin and membrane work in synergy to produce the electrical and mechanical changes that are required during OHC electromotility... - [Preparation and characterization of monoclonal antibody specific to PfCP-2.9 chimeric protein of Plasmodium falciparum]Rui Wang
Department of Etiologic Biology, The Second Military Medical University, Shanghai, China
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 24:247-50. 2006..3 mg/ml. CONCLUSION: A monoclonal antibody against PfCP-2.9 malaria vaccine candidate has been obtained, which recognizes a conformational epitope of the protein and natural protein... - Conjugating recombinant proteins to Pseudomonas aeruginosa ExoProtein A: a strategy for enhancing immunogenicity of malaria vaccine candidatesFeng Qian
Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Rockville, MD 20852, USA
Vaccine 25:3923-33. 2007..These conjugates now need to be tested in humans to determine if mice are predictive of the response in humans... - A splice form of polycystin-2, lacking exon 7, does not interact with polycystin-1Karl Hackmann
Institut fur Humangenetik, Universitatsklinikum Munster, Germany
Hum Mol Genet 14:3249-62. 2005..The predominant expression in brain indicates a function in this organ. The inability to interact with polycystin-1 expands further the PKD1-independent functions of polycystin-2 forms... - Expression of PKD1 and PKD2 transcripts and proteins in human embryo and during normal kidney developmentVeronique Chauvet
INSERM U423 and the Département deGénétique et Unité INSERM U393, Hopital Necker Enfants Malades, Paris, France
Am J Pathol 160:973-83. 2002..These data suggest that polycystins could interact with different partners, at least during kidney development... - [Enhancement by an in vivo-activated promoter of immunogenicity of recombinant attenuated Salmonella typhimurium expressing hepatitis C virus core antigen]Ping Zhao
Department of Microbiology, Second Military Medical University, Shanghai 200433, China
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:266-70. 2003.... - [Determination of free thiols in the chimeric protein PfCP-2.9 of Plasmodium falciparum]Feng Qian
Department of Etiological Biology, Second Military Medical University, Shanghai 200433
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 21:99-101. 2003..9 and indoacetic acid-alkylated PfCP-2.9. RESULTS: Both experiments showed that there were no any free thiols present in the PfCP-2.9. CONCLUSION: The disulfide bonds between cysteine residues of PfCP-2.9 were formed completely... - NY-ESO-1 and LAGE-1 cancer-testis antigens are potential targets for immunotherapy in epithelial ovarian cancerKunle Odunsi
Division of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
Cancer Res 63:6076-83. 2003..These findings indicate that NY-ESO-1 and LAGE-1 are attractive targets for antigen-specific immunotherapy in EOC... - pPIC9-Fc: a vector system for the production of single-chain Fv-Fc fusions in Pichia pastoris as detection reagents in vitroJianglan Liu
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China
J Biochem (Tokyo) 134:911-7. 2003.... - [Relationship between efficacy of interferon-alpha and phenotypes of peripheral blood dendritic cells in patients with chronic hepatitis B]Chuan-wu Zhu
Department of Infectious Diseases, Fifth People's Hospital of Suzhou, Suzhou 215007, China
Zhonghua Gan Zang Bing Za Zhi 12:174-5. 2004 - Fusion of two malaria vaccine candidate antigens enhances product yield, immunogenicity, and antibody-mediated inhibition of parasite growth in vitroWeiqing Pan
Department of Etiologic Biology, Second Military Medical University, Shanghai, China
J Immunol 172:6167-74. 2004..9. The combination of the extremely high yield of the protein and enhancement of its immune response provides a basis to develop an effective and affordable malaria vaccine... - Identification of differentially expressed genes in clinically distinct groups of serous ovarian carcinomas using cDNA microarrayYvonne Collins
Department of Gynecologic Oncology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14261, USA
Int J Mol Med 14:43-53. 2004..Our study demonstrates differential gene expression in clinically distinct groups of SEOC using cDNA microarray. These genes may potentially be useful as biomarkers and/or targets for therapeutic intervention... - [Views on sequence variations of AMA-1 in Plasmodium falciparum FCC1/HN isolate]Feng Qian
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22:256. 2004 - In vivo antitumor activity by 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone in a solid human carcinoma xenograft modelChun-Lin Ye
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China
Cancer Chemother Pharmacol 56:70-4. 2005..60+/-0.80% of the total in mice injected with 150 mg/kg DMC). To our knowledge, this is the first time that chalcone compounds have been applied to a human tumor xenograft model... - TRP genes: candidates for nonselective cation channels and store-operated channels in insulin-secreting cellsFeng Qian
Department of Neurobiology, Physiology, and Pharmacology, The University of Chicago, Chicago, Illinois, USA
Diabetes 51:S183-9. 2002..TRPC-like channels may provide a pathway for depolarization or Ca(2+) entry in beta-cells and may be interesting targets for manipulating beta-cell function...
Research Grants
- The Proteolytic Cleavage of Polycystin-1: How and WhyFeng Qian; Fiscal Year: 2007..The information from our studies will likely open new avenues in the research of ADPKD and establish the foundation for developing causative and effective therapies of the disease. ..
- The Proteolytic Cleavage of Polycystin-1: How and WhyFeng Qian; Fiscal Year: 2010..This study aims to understand Polycystin-1, the reasons it becomes defective, and the ways to prevent it. ..
- MOLECULAR GENETICS OF HUMAN ARPKDGregory Germino; Fiscal Year: 2004..In parallel, we will produce antibodies to the PKHD1 gene product and characterize the protein's pattern of expression in Aim number 4. Finally, we will create a murine model of PKHD1 using gene-targeting methods. ..
- MOLECULAR GENETICS OF HUMAN ARPKDGregory Germino; Fiscal Year: 2007..abstract_text> ..
- NOVEL APPROACH TO THE MOLECULAR GENETICS OF PKD1Gregory Germino; Fiscal Year: 2003..The latter will help define the pathophysiology of this disorder, allow the development and testing of therapeutic compounds and allow manipulation of the environmental and genetic background to assess the relative effects of each. ..
- MOLECULAR GENETICS OF HUMAN ARPKDGregory Germino; Fiscal Year: 1999..The ultimate selection of a method will depend on the size of the gene and the rate of progress in the development of newer, more efficient technologies. ..
- The Proteolytic Cleavage of Polycystin-1: How and WhyFeng Qian; Fiscal Year: 2010..This study aims to understand Polycystin-1, the reasons it becomes defective, and the ways to prevent it. ..