C M Pickart

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi Mechanisms underlying ubiquitination
    C M Pickart
    School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
    Annu Rev Biochem 70:503-33. 2001
  2. ncbi Ubiquitin enters the new millennium
    C M Pickart
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell 8:499-504. 2001
  3. ncbi Ubiquitin in chains
    C M Pickart
    Dept of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Trends Biochem Sci 25:544-8. 2000
  4. ncbi In vitro assembly and recognition of Lys-63 polyubiquitin chains
    R M Hofmann
    Department of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 276:27936-43. 2001
  5. ncbi Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer
    A P VanDemark
    Department of Biophysics and Biophysical Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
    Cell 105:711-20. 2001
  6. ncbi A HECT domain E3 enzyme assembles novel polyubiquitin chains
    J You
    Department of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 276:19871-8. 2001
  7. ncbi Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair
    R M Hofmann
    Department of Biochemistry, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Cell 96:645-53. 1999
  8. ncbi Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes
    F G Whitby
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84132, USA
    J Biol Chem 273:34983-91. 1998
  9. ncbi Mechanism of ubiquitin conjugating enzyme E2-230K: catalysis involving a thiol relay?
    E S Berleth
    Department of Biochemistry, State University of New York at Buffalo 14214, USA
    Biochemistry 35:1664-71. 1996
  10. ncbi Core domain mutation (S86Y) selectively inactivates polyubiquitin chain synthesis catalyzed by E2-25K
    L D Mastrandrea
    Department of Biochemistry, State University of New York, Buffalo 14214, USA
    Biochemistry 37:9784-92. 1998

Collaborators

  • C Wolberger
  • R M Hofmann
  • J You
  • A P VanDemark
  • L D Mastrandrea
  • I Wefes
  • F G Whitby
  • E S Berleth
  • G Xia
  • M A Kalchman
  • C Tsui
  • D Finley
  • E G Niles
  • E M Kasperek
  • C P Hill
  • J G Hodgson
  • K C Graham
  • R D Gietz
  • Y P Goldberg
  • R K Graham
  • M R Hayden
  • H B Koide
  • P Kaiser
  • M Haldeman
  • J Tamburlin
  • S T Koury
  • R Schneider
  • W J Cook
  • L C Jeffrey
  • Z J Chen
  • Z Chen
  • M Carson

Detail Information

Publications16

  1. ncbi Mechanisms underlying ubiquitination
    C M Pickart
    School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
    Annu Rev Biochem 70:503-33. 2001
    ..The new findings shed light on many aspects of E3 structure, function, and mechanism, but also emphasize that key features of E3 catalysis remain to be elucidated...
  2. ncbi Ubiquitin enters the new millennium
    C M Pickart
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell 8:499-504. 2001
    ..New findings show that besides signaling proteolysis, ubiquitination can be a signal for trafficking, kinase activation, and other nonproteolytic fates...
  3. ncbi Ubiquitin in chains
    C M Pickart
    Dept of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Trends Biochem Sci 25:544-8. 2000
    ..Recent results have revealed important determinants of polyubiquitin-chain recognition by proteasomes, helping to explain the biological rationale behind this novel signaling mechanism...
  4. ncbi In vitro assembly and recognition of Lys-63 polyubiquitin chains
    R M Hofmann
    Department of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 276:27936-43. 2001
    ....
  5. ncbi Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer
    A P VanDemark
    Department of Biophysics and Biophysical Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
    Cell 105:711-20. 2001
    ..Selective mutations in the channels suggest a molecular model for specificity in the assembly of Lys63-linked polyubiquitin signals...
  6. ncbi A HECT domain E3 enzyme assembles novel polyubiquitin chains
    J You
    Department of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 276:19871-8. 2001
    ....
  7. ncbi Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair
    R M Hofmann
    Department of Biochemistry, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Cell 96:645-53. 1999
    ..These findings support a model in which an Mms2p/Ubc13p complex assembles novel polyubiquitin chains for signaling in DNA repair, and they suggest that UEV proteins may act to increase diversity and selectivity in ubiquitin conjugation...
  8. ncbi Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes
    F G Whitby
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84132, USA
    J Biol Chem 273:34983-91. 1998
    ..One such residue, Ala-72 (Arg in ubiquitin), is shown to perform a key role in selecting against reaction with the ubiquitin E1 enzyme, thereby acting to prevent the inappropriate diversion of NEDD8 into ubiquitin-specific pathways...
  9. ncbi Mechanism of ubiquitin conjugating enzyme E2-230K: catalysis involving a thiol relay?
    E S Berleth
    Department of Biochemistry, State University of New York at Buffalo 14214, USA
    Biochemistry 35:1664-71. 1996
    ..In this model, E2-230K functions as an E2-E3 hybrid...
  10. ncbi Core domain mutation (S86Y) selectively inactivates polyubiquitin chain synthesis catalyzed by E2-25K
    L D Mastrandrea
    Department of Biochemistry, State University of New York, Buffalo 14214, USA
    Biochemistry 37:9784-92. 1998
    ..These results suggest that unique residues proximal to E2 active sites may influence specific function by mediating intramolecular interactions...
  11. ncbi Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme
    M A Kalchman
    Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
    J Biol Chem 271:19385-94. 1996
    ..The huntingtin-E2-25K interaction is not obviously modulated by CAG length. We also demonstrate that huntingtin is ubiquitinated. These findings have implications for the regulated catabolism of the gene product for HD...
  12. pmc Induction of ubiquitin-conjugating enzymes during terminal erythroid differentiation
    I Wefes
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 92:4982-6. 1995
    ..These data implicate regulated components of the ubiquitin conjugation machinery in erythroid differentiation...
  13. ncbi Characterization of a cDNA clone encoding E2-20K, a murine ubiquitin-conjugating enzyme
    I Wefes
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Gene 163:321-2. 1995
    ..Here we report the sequence of a murine cDNA encoding E2-20K. Northern blot analysis identified polyadenylated transcripts of 3.5 and 6.5 kb which are present at comparable levels in many nonerythroid tissues...
  14. ncbi Several mammalian ubiquitin carrier proteins, but not E2(20K), are related to the 20-kDa yeast E2, RAD6
    E S Berleth
    Department of Biochemistry, SUNY, Buffalo 14214
    Biochem Biophys Res Commun 171:705-10. 1990
    ..RAD6 and E2-20K were also shown to multi-ubiquitinate histones by different mechanisms...
  15. ncbi Isolation of a cDNA encoding a mammalian multiubiquitinating enzyme (E225K) and overexpression of the functional enzyme in Escherichia coli
    Z J Chen
    Department of Biochemistry, State University of New York, Buffalo 14214
    J Biol Chem 266:15698-704. 1991
    ..The availability of a cloned cDNA will allow us to assess the physiological role of E2(25K)...
  16. ncbi Structure of a diubiquitin conjugate and a model for interaction with ubiquitin conjugating enzyme (E2)
    W J Cook
    Department of Pathology, University of Alabama, Birmingham 35294
    J Biol Chem 267:16467-71. 1992
    ..The structure of the diubiquitin conjugate suggests determinants for recognition of multiubiquitin chains. A model for the interaction of diubiquitin and a ubiquitin conjugating enzyme (E2) is proposed...