Research Topics
Genomes and GenesSpecies | D J MontellSummaryAffiliation: Johns Hopkins University Country: USA Publications
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Publications
Modeling migration and metastasis in DrosophilaAnna C C Jang
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
J Mammary Gland Biol Neoplasia 12:103-14. 2007..Together these studies suggest that Drosophila has much to offer as a model for varied aspects of tumor metastasis...- A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imagingMohit Prasad
Department of Biological Chemistry, Center for Cell Dynamics, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
Nat Protoc 2:2467-73. 2007..The entire protocol takes approximately 24 h while the preparation of egg chambers for live imaging requires only 15-20 min... - Cellular and molecular mechanisms of border cell migration analyzed using time-lapse live-cell imagingMohit Prasad
Department of Biological Chemistry, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
Dev Cell 12:997-1005. 2007..These observations provide new insight into the cellular dynamics and molecular mechanisms of cell migration in vivo... 
Group choreography: mechanisms orchestrating the collective movement of border cellsDenise J Montell
Department of Biological Chemistry, Center for Cell Dynamics, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Nat Rev Mol Cell Biol 13:631-45. 2012..Here we provide an overview of the molecular choreography of border cells and its more general implications...- The genetics of cell migration in Drosophila melanogaster and Caenorhabditis elegans developmentD J Montell
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
Development 126:3035-46. 1999..New types of genetic screens promise to fill in some of these gaps in the near future... - A kinase gets caspases into shapeDenise J Montell
Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA
Cell 126:450-2. 2006..However, DmIKK epsilon-mediated degradation of DIAP1 does not regulate apoptosis as might be predicted but instead regulates actin dynamics, cell morphology, and the differentiation of sensory organ precursor cells... - Command and control: regulatory pathways controlling invasive behavior of the border cellsD J Montell
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, 21205 2185, Baltimore, MD, USA
Mech Dev 105:19-25. 2001..This may contribute to the metastatic effects of steroid hormones on certain cancers and the inhibition of metastasis by steroid hormone antagonists such as tamoxifen... 
Metastasis movies, macrophages, molecules and more. Conference on Mechanisms of Invasion and MetastasisDenise Montell
Department of Biological Chemistry, School of Medicine, Johns Hopkins University, 725 North Wolfe Street, 408 WBSB, Baltimore, Maryland 21205, USA
EMBO Rep 4:458-62. 2003- Morphogenetic cell movements: diversity from modular mechanical propertiesDenise J Montell
Department of Biological Chemistry, Center for Cell Dynamics, Rangos Building, Suite 450, 855 North Wolfe Street, Baltimore, MD 21205, USA
Science 322:1502-5. 2008..A small number of properties used in combination could, in principle, generate a diverse array of cell shapes and arrangements and thus orchestrate the varied morphogenetic events observed during metazoan organ development... - Border-cell migration: the race is onDenise J Montell
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
Nat Rev Mol Cell Biol 4:13-24. 2003..Three distinct signals, which are also upregulated in cancer, control border-cell migration, so identifying further genes that are involved in border-cell migration could provide new insights into tumour invasion... - Jing: a downstream target of slbo required for developmental control of border cell migrationY Liu
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
Development 128:321-30. 2001..We propose that the need to coordinate cell differentiation with nutritional status may be the link between mammalian adipocytes and Drosophila border cells that led to the conservation of C/EBP and AEBP2... - Paracrine signaling through the JAK/STAT pathway activates invasive behavior of ovarian epithelial cells in DrosophilaD L Silver
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Cell 107:831-41. 2001..Ectopic expression of either UPD or JAK is sufficient to induce extra epithelial cells to migrate. Thus, a localized signal activates the JAK/STAT pathway in neighboring epithelial cells, causing them to become invasive... - Regulation of invasive cell behavior by taiman, a Drosophila protein related to AIB1, a steroid receptor coactivator amplified in breast cancerJ Bai
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Cell 103:1047-58. 2000..The finding of this type of coactivator required for cell motility suggests a novel role for steroid hormones, in stimulating invasive cell behavior, independent of effects on proliferation... - Two distinct roles for Ras in a developmentally regulated cell migrationT Lee
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Development 122:409-18. 1996..Raf activity was dispensable to border cell migration while reduced Ral activity inhibited initiation. We therefore suggest that Ras plays a critical role in the dynamic regulation of border cell migration via a Raf-independent pathway... - Regulated Breathless receptor tyrosine kinase activity required to pattern cell migration and branching in the Drosophila tracheal systemT Lee
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205 2185, USA
Genes Dev 10:2912-21. 1996.... - Multiple Ras signals pattern the Drosophila ovarian follicle cellsT Lee
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205 2185, USA
Dev Biol 185:25-33. 1997..These results suggest that, in vivo as in vitro, Ras can have diverse effects on different cells, but, in addition, Ras activity can have different effects on the same cells at different stages in their development... - Developmental regulation of cell migration. Insight from a genetic approach in DrosophilaD J Montell
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205 2185, USA
Cell Biochem Biophys 31:219-29. 1999..Finally, genetic studies have demonstrated that the timing of cell migration is under transcriptional control... - Identification of mutations that cause cell migration defects in mosaic clonesY Liu
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
Development 126:1869-78. 1999..This screen identified many new loci required for border cell migration and our results suggest that this is a useful approach for elucidating the mechanisms involved in cell motility... - Requirement for the vasa RNA helicase in gurken mRNA localizationR Tinker
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205 2185, USA
Dev Biol 199:1-10. 1998.... - Cell type-specific roles for Cdc42, Rac, and RhoL in Drosophila oogenesisA M Murphy
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205 2185, USA
J Cell Biol 133:617-30. 1996..All three activities were necessary for normal transfer of nurse cell cytoplasm to the oocyte. These results suggest that Rho protein activities have cell type-specific effects on morphogenesis... - The breathless FGF receptor homolog, a downstream target of Drosophila C/EBP in the developmental control of cell migrationA M Murphy
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
Development 121:2255-63. 1995..Purified Drosophila C/EBP bound eight sites in the btl 5' flanking region by DNAse I footprinting. Taken together these results suggest that btl is a key, direct target for C/EBP in the regulation of border cell migration... - A Drosophila derailed homolog, doughnut, expressed in invaginating cells during embryogenesisS Savant-Bhonsale
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Gene 231:155-61. 1999..These results suggest that DNT associates with a catalytically active kinase, but may not be capable of autophosphorylation... - Requirement for Par-6 and Bazooka in Drosophila border cell migrationElaine M Pinheiro
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Development 131:5243-51. 2004..Taken together, these results indicate that cells need not lose apical/basal polarity in order to invade neighboring tissues and in some cases even require such polarity for proper motility... - Moving right along: regulation of cell migration during Drosophila developmentD J Montell
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205
Trends Genet 10:59-62. 1994..Signaling through receptor tyrosine kinases may be a general mechanism for the regulation of cell movement in development and in metastasis... - torso-like encodes the localized determinant of Drosophila terminal pattern formationS Savant-Bhonsale
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Genes Dev 7:2548-55. 1993..Ectopic expression of tsl produced embryos with a phenotype similar to that resulting from constitutively active Tor alleles. These results suggest that localized TSL controls the localized activation of TOR... - A role for extra macrochaetae downstream of Notch in follicle cell differentiationJennifer C Adam
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Development 131:5971-80. 2004..We propose that EMC functions downstream of Notch and upstream of EYA to regulate main body cell fate specification and differentiation... - Multiple EGFR ligands participate in guiding migrating border cellsJocelyn A McDonald
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Dev Biol 296:94-103. 2006..In addition, only Keren and Spitz were expressed at the appropriate stage in the oocyte, the target of border cell migration. Therefore, a complex combination of EGFR and PVR ligands together guide border cells to the oocyte... - Requirement for JAK/STAT signaling throughout border cell migration in DrosophilaDebra L Silver
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 212052, USA
Development 132:3483-92. 2005..Together, our findings suggest that the requirement for STAT in border cells extends beyond the initial specification and delamination of cells from the epithelium... - A role for Drosophila IAP1-mediated caspase inhibition in Rac-dependent cell migrationErika R Geisbrecht
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
Cell 118:111-25. 2004..These results indicate an apoptosis-independent role for DIAP1-mediated Dronc inhibition in Rac-mediated cell motility... - Feedback inhibition of Jak/STAT signaling by apontic is required to limit an invasive cell populationMichelle Starz-Gaiano
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 855 North Wolfe Street, Baltimore, MD 21205, USA
Dev Cell 14:726-38. 2008..These findings are supported by a mathematical model, which accurately simulates wild-type and mutant phenotypes... - Myosin VI is required for E-cadherin-mediated border cell migrationErika R Geisbrecht
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Nat Cell Biol 4:616-20. 2002..Multiple biochemical mechanisms may exist, therefore, for cadherins to associate with diverse unconventional myosins that are required for normal stereocilium formation or maintenance... - Analysis of cell migration using Drosophila as a model systemJocelyn A McDonald
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Methods Mol Biol 294:175-202. 2005..In addition, we provide protocols for staining embryos and manipulating gene function in each of the migratory populations. Finally, we offer some advice concerning the analysis and interpretation of mutant phenotypes... - Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovaryXuejiao Wang
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
Dev Cell 10:483-95. 2006..Functional analysis revealed a role for the Notch-activating protease Kuzbanian in border cell migration and identified Tie as a guidance receptor for the border cells... - Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivoXiaobo Wang
Department of Biological Chemistry, Center for Cell Dynamics, Johns Hopkins School of Medicine, 855 North Wolfe Street, Baltimore, MD 21205 USA
Nat Cell Biol 12:591-7. 2010..Communication between cells of the cluster required Jun amino-terminal kinase (JNK) but not guidance receptor signalling. These studies further show that photoactivatable proteins are effective tools in vivo... - A new trick for Cyclin-Cdk: activation of STATDebra L Silver
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 Wolfe Street, Baltimore, MD 21205, USA
Dev Cell 4:148-9. 2003..Cdk4 overexpression can bypass requirements for JAK but not STAT. These results demonstrate a new function for Cdk4 and a new mode of STAT activation... - The social lives of migrating cells in DrosophilaDenise J Montell
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205-2185, USA
Curr Opin Genet Dev 16:374-83. 2006..And in border cells, DIAP1 and Dronc, two proteins known mainly for their ability to regulate cell death, were found to control cell migration... - Genes that drive invasion and migration in DrosophilaMichelle Starz-Gaiano
Department of Biological Chemistry, Johns Hopkins Medical Institute, Wood Basic Science Building 413, 725 N Wolfe Street, Baltimore, MD 21205, USA
Curr Opin Genet Dev 14:86-91. 2004..Comparison of genes that regulate these processes to those that promote tumorigenesis and metastasis in mammals demonstrates that studies in fruit flies are uncovering new genes highly relevant to cancer biology... - PVF1, a PDGF/VEGF homolog, is sufficient to guide border cells and interacts genetically with TaimanJocelyn A McDonald
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Development 130:3469-78. 2003..In addition, we find that taiman and Pvf1 regulate the dynamic localization of E-cadherin in the border cells, possibly accounting for the interaction between these two pathways... - Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motilityDebra L Silver
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Cancer Res 64:3550-8. 2004..These results suggest that Janus-activated kinase/STAT signaling may contribute to ovarian cancer cell invasiveness... - Anchors away! Fos fosters anchor-cell invasionDenise J Montell
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
Cell 121:816-7. 2005..They demonstrate that the transcription factor Fos is required for cell invasion and identify three of its downstream target genes (encoding a matrix metalloproteinase, hemicentin, and a fat-like protocadherin)... - Ovarian cancer metastasis: integrating insights from disparate model organismsHonami Naora
Department of Molecular Therapeutics, University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Nat Rev Cancer 5:355-66. 2005..Genetic analysis of border-cell migration in the Drosophila melanogaster ovary provides clues that will improve our understanding of ovarian cancer metastasis at the molecular level, and also might lead to potential therapeutic targets...
Research Grants
- Adhesion dynamics in Drosophila border cell migrationDenise Montell; Fiscal Year: 2007..Finally we propose to study a putative downstream target of Rho in border cells, rhophilin by characterizing the mutant phenotype, epistasis analysis with Rho and by identifying and characterizing interacting proteins. ..
- REGULATION OF CELL MIGRATION IN DEVELOPMENTDenise Montell; Fiscal Year: 2007..We will also investigate the biochemical mechansims that tether UPD to the cell membrane. ..
- Cytoskeleton Dynamics in Developmental Cell MigrationDenise J Montell; Fiscal Year: 2010..Since cell motility contributes to wound healing and tumor metastasis, these studies could lead to the discovery of new drug targets that could promote healing or inhibit the spread of cancer cells. ..
- REGULATION OF CELL MIGRATION IN DEVELOPMENTDenise J Montell; Fiscal Year: 2010..Since cell motility contributes to wound healing and tumor metastasis, these studies could lead to the discovery of new drug targets that could promote healing or inhibit the spread of cancer cells. ..
- REGULATION OF CELL MIGRATION IN DEVELOPMENTDenise Montell; Fiscal Year: 1999..We also propose to test the hypotheses that Dts3 associates with the cytoskeleton and is regulated by tyrosine phosphorylation. ..
- REGULATION OF CELL MIGRATION IN DEVELOPMENTDenise Montell; Fiscal Year: 2003....
- REGULATION OF CELL MIGRATION IN DEVELOPMENTDenise Montell; Fiscal Year: 2009..Since cell motility contributes to wound healing and tumor metastasis, these studies could lead to the discovery of new drug targets that could promote healing or inhibit the spread of cancer cells. ..