D MacGlashan

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Synthetic IgE peptide vaccine for immunotherapy of allergy
    Chang Yi Wang
    United Biomedical Inc, 25 Davids Drive, Hauppauge, NY 11788, USA
    Vaccine 21:1580-90. 2003
  2. pmc Suppression of the basophil response to allergen during treatment with omalizumab is dependent on 2 competing factors
    Donald W Macglashan
    Division of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University, Baltimore, MD, USA
    J Allergy Clin Immunol 130:1130-1135.e5. 2012
  3. pmc IgE-dependent signaling as a therapeutic target for allergies
    Donald W Macglashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    Trends Pharmacol Sci 33:502-9. 2012
  4. pmc Development of a microarray-based method to detect exposure of human basophils to IL-3
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, United States
    J Immunol Methods 385:51-9. 2012
  5. pmc Subthreshold desensitization of human basophils re-capitulates the loss of Syk and FcεRI expression characterized by other methods of desensitization
    D MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    Clin Exp Allergy 42:1060-70. 2012
  6. doi request reprint Inducing an anergic state in mast cells and basophils without secretion
    Donald MacGlashan
    Asthma and Allergy Center, Johns Hopkins University, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 121:1500-6, 1506.e1-4. 2008
  7. ncbi request reprint Loss of syk kinase during IgE-mediated stimulation of human basophils
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 114:1317-24. 2004
  8. ncbi request reprint Induced loss of Syk in human basophils by non-IgE-dependent stimuli
    Donald W Macglashan
    Asthma and Allergy Center, Johns Hopkins University, Baltimore, MD 21224, USA
    J Immunol 180:4208-17. 2008
  9. ncbi request reprint Endocytosis, recycling, and degradation of unoccupied FcepsilonRI in human basophils
    Donald W Macglashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Leukoc Biol 82:1003-10. 2007
  10. ncbi request reprint Relationship between spleen tyrosine kinase and phosphatidylinositol 5' phosphatase expression and secretion from human basophils in the general population
    Donald W Macglashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 119:626-33. 2007

Research Grants

  1. MECHANISMS OF HUMAN BASOPHIL & MAST CELL DESENSITIZATION
    Donald MacGlashan; Fiscal Year: 1992
  2. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2003
  3. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2007
  4. MECHANISMS OF HUMAN BASOPHIL & MAST CELL DESENSITIZATION
    Donald MacGlashan; Fiscal Year: 1993
  5. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2009

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Synthetic IgE peptide vaccine for immunotherapy of allergy
    Chang Yi Wang
    United Biomedical Inc, 25 Davids Drive, Hauppauge, NY 11788, USA
    Vaccine 21:1580-90. 2003
    ..Immunized dogs experienced significant reductions in total serum IgE...
  2. pmc Suppression of the basophil response to allergen during treatment with omalizumab is dependent on 2 competing factors
    Donald W Macglashan
    Division of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University, Baltimore, MD, USA
    J Allergy Clin Immunol 130:1130-1135.e5. 2012
    ..However, there were differences that provided additional insight into the nature of the cellular responses in allergic subjects...
  3. pmc IgE-dependent signaling as a therapeutic target for allergies
    Donald W Macglashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    Trends Pharmacol Sci 33:502-9. 2012
    ..This overview will focus on events following activation of the high-affinity receptor because FcɛRI generates the classical immediate hypersensitivity reaction...
  4. pmc Development of a microarray-based method to detect exposure of human basophils to IL-3
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, United States
    J Immunol Methods 385:51-9. 2012
    ..Finally, these studies provided an estimate for the normal in vivo exposure of circulating basophils to IL-3 or IL-3-like functionality. These studies identified an IL-3 signature to probe changes in basophils occurring in vivo...
  5. pmc Subthreshold desensitization of human basophils re-capitulates the loss of Syk and FcεRI expression characterized by other methods of desensitization
    D MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    Clin Exp Allergy 42:1060-70. 2012
    ..However, most signalling studies of human basophils have identified changes in signalling by using other methods of inducing cellular desensitization...
  6. doi request reprint Inducing an anergic state in mast cells and basophils without secretion
    Donald MacGlashan
    Asthma and Allergy Center, Johns Hopkins University, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 121:1500-6, 1506.e1-4. 2008
    ..IgE-mediated secretion from mast cells or basophils depends on the activity of both spleen tyrosine kinase (syk) and phosphatidyl inositol 3' kinase (PI3K), but several specific downregulatory pathways (eg, loss of syk expression) do not...
  7. ncbi request reprint Loss of syk kinase during IgE-mediated stimulation of human basophils
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 114:1317-24. 2004
    ..Recent studies have focused on downregulatory steps that appear to modify the presence of the activated state of various signaling molecules. We now examine downregulation regulated by mechanisms related to proteasome processing...
  8. ncbi request reprint Induced loss of Syk in human basophils by non-IgE-dependent stimuli
    Donald W Macglashan
    Asthma and Allergy Center, Johns Hopkins University, Baltimore, MD 21224, USA
    J Immunol 180:4208-17. 2008
    ..These studies demonstrate that it is possible to induce the loss of Syk expression in human basophils by a non-IgE-dependent mechanism and even by a mechanism that does directly involve Syk in the reaction complex...
  9. ncbi request reprint Endocytosis, recycling, and degradation of unoccupied FcepsilonRI in human basophils
    Donald W Macglashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Leukoc Biol 82:1003-10. 2007
    ..These studies establish that the unoccupied receptor is lost by a process of endocytosis, partially recycled to the cell surface, and ultimately degraded by a lysosomal mechanism...
  10. ncbi request reprint Relationship between spleen tyrosine kinase and phosphatidylinositol 5' phosphatase expression and secretion from human basophils in the general population
    Donald W Macglashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 119:626-33. 2007
    ..Previous studies have suggested that expression levels of spleen tyrosine kinase (syk) or phosphatidylinositol 5' phosphatase (SHIP) may explain certain extreme human basophil phenotypes...
  11. ncbi request reprint IgE and Fc{epsilon}RI regulation
    Donald MacGlashan
    The Johns Hopkins Asthma and Allergy Center, 5501 Bayview Circle, Baltimore, MD 21224, USA
    Ann N Y Acad Sci 1050:73-88. 2005
    ..Of particular interest for understanding the variation in expression in atopy among patients, the role of this receptor on non-mast cell/basophils will be important to elucidate...
  12. ncbi request reprint Nonspecific desensitization, functional memory, and the characteristics of SHIP phosphorylation following IgE-mediated stimulation of human basophils
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Center, Baltimore, MD 21224, USA
    J Immunol 177:1040-51. 2006
    ....
  13. ncbi request reprint Two regions of down-regulation in the IgE-mediated signaling pathway in human basophils
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Immunol 170:4914-25. 2003
    ....
  14. pmc Polymerization of actin does not regulate desensitization in human basophils
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Leukoc Biol 85:627-37. 2009
    ..These studies demonstrate that desensitization is not the result of two signaling pathways once considered relevant to down-regulation of IgE-mediated signaling...
  15. doi request reprint Self-termination/anergic mechanisms in human basophils and mast cells
    Donald W Macglashan
    Asthma and Allergy Center, Johns Hopkins University, Baltimore, MD 21224, USA
    Int Arch Allergy Immunol 150:109-21. 2009
    ..But many negative feedback loops become active only after the cell is stimulated. By and large, it will be these processes that will be discussed in this review...
  16. pmc Inhibition of IgE-mediated secretion from human basophils with a highly selective Bruton's tyrosine kinase, Btk, inhibitor
    Donald MacGlashan
    Johns Hopkins University, Asthma and Allergy Center, Baltimore, MD 21224, USA
    Int Immunopharmacol 11:475-9. 2011
    ..These results suggest that btk is specifically required for IgE-mediated activation of human basophils...
  17. ncbi request reprint IgE-regulated loss, not IgE-regulated synthesis, controls expression of FcepsilonRI in human basophils
    D MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, Maryland 21224, USA
    J Leukoc Biol 70:207-18. 2001
    ..Taken together, these results suggest that IgE regulates cell surface expression of FcepsilonRI only by regulating the rate that receptor is lost from the cell surface...
  18. pmc Expression of CD203c and CD63 in human basophils: relationship to differential regulation of piecemeal and anaphylactic degranulation processes
    D MacGlashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Clin Exp Allergy 40:1365-77. 2010
    ..The markers CD63 and CD203c have been used in recent years to assess basophil activation but there have been many studies that demonstrate that expression of these markers can be dissociated from histamine release...
  19. doi request reprint IgE receptor and signal transduction in mast cells and basophils
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, United States
    Curr Opin Immunol 20:717-23. 2008
    ..Understanding more about the biology of the receptor and the signaling that it initiates remains important to the development of new therapeutic approaches to inhibit this reaction...
  20. ncbi request reprint IgE and FcepsilonRI regulation
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD, USA
    Clin Rev Allergy Immunol 29:49-60. 2005
    ..The control of FcepsilonRI expression, control of cell function by FcepsilonRI, and expression of FcepsilonRI on other cell types are important new areas of understanding currently being explored...
  21. ncbi request reprint Pharmacology of IgE-mediated desensitization of human basophils: effects of protein kinase C and src-family kinase inhibitors
    D MacGlashan
    Johns Hopkins Asthma and Allergy Center, 21224, USA, Baltimore, MD, USA
    Biochem Pharmacol 60:1717-27. 2000
    ..These data suggest that PKC has little role in down-regulating the IgE-mediated basophil response. However, like the activation signaling cascade, the desensitization process is dependent on the activation of src family kinases...
  22. ncbi request reprint Characteristics of the free cytosolic calcium timelag following IgE-mediated stimulation of human basophils: significance for the nonreleasing basophil phenotype
    D MacGlashan
    Johns Hopkins University, Asthma and Allergy Center, Baltimore, Maryland 21224, USA
    J Leukoc Biol 69:224-32. 2001
    ..However, only one characteristic of the [Ca++]i response phenotype of nonreleasing basophils--the [Ca++]i timelag but not the absence of a sustained [Ca++]i elevation--could be mimicked by inhibition of lyn kinase with PP1...
  23. ncbi request reprint Functional consequences of FcepsilonRIalpha up-regulation by IgE in human basophils
    D MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, Maryland 21224, USA
    J Leukoc Biol 68:479-86. 2000
    ..These results indicate that functional up-regulation does occur but that its magnitude may be modulated because not all components of the signaling pathway are up-regulated in a balanced manner...
  24. ncbi request reprint Desensitization of IgE-mediated IL-4 release from human basophils
    D MacGlashan
    Johns Hopkins Allergy and Asthma Center, Baltimore, Maryland 21224, USA
    J Leukoc Biol 63:59-67. 1998
    ..e., stimulation in the presence of Ca(e), these results also indicate that down-regulatory processes differ depending on the presence or absence of Ca(e)...
  25. ncbi request reprint IgE-mediated desensitization in human basophils and mast cells
    D MacGlashan
    Johns Hopkins University, Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Front Biosci 3:d746-56. 1998
    ..A variety of processes are discussed and potential mechanisms based on most recent studies using cell lines are explored...
  26. ncbi request reprint Transient transfection of human peripheral blood basophils
    Natalia Vilariño
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Immunol Methods 296:11-8. 2005
    ....
  27. ncbi request reprint Basophils in airway disease
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Curr Allergy Asthma Rep 2:126-32. 2002
    ..Coupled with new insights into cytokine secretion from these cells, a more accurate picture of the dynamics of this specialized form of inflammation is available for refining our hypotheses regarding its regulation...
  28. doi request reprint Early signal protein expression profiles in basophils: a population study
    Susan Ishmael
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Leukoc Biol 86:313-25. 2009
    ....
  29. ncbi request reprint Omalizumab-induced reductions in mast cell Fce psilon RI expression and function
    Lisa A Beck
    Division of Clinical Immunology, Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 114:527-30. 2004
    ..It is assumed that tissue mast cells are similarly altered in their Fc epsilon RI density and function...
  30. pmc Signaling cascades: escape from kinetic proofreading
    D MacGlashan
    Johns Hopkins University Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA dmacglas mail jhmi edu
    Proc Natl Acad Sci U S A 98:6989-90. 2001
  31. ncbi request reprint Histamine: A mediator of inflammation
    Donald MacGlashan
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 112:S53-9. 2003
    ..It has not yet been determined whether this action represents a class effect...
  32. ncbi request reprint Anti-IgE antibody therapy
    Donald MacGlashan
    Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Clin Allergy Immunol 16:519-32. 2002
    ..Some concerns still revolve around the improvement roles IgE may play in parasitic diseases, the cost of therapy, and that very long-term treatment effects have yet to be studied...
  33. pmc Syk expression in peripheral blood leukocytes, CD34+ progenitors, and CD34-derived basophils
    Susan S Ishmael
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Leukoc Biol 87:291-300. 2010
    ....
  34. ncbi request reprint Actin cytoskeleton-dependent down-regulation of early IgE-mediated signaling in human basophils
    Natalia Vilariño
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD, USA
    J Leukoc Biol 75:928-37. 2004
    ..Moreover, the fact that latrunculin A did not result in sustained Erk phosphorylation supports the presence of a second down-regulatory step between syk and Erk that cannot be overcome by a sustained early signal...
  35. ncbi request reprint Leukocyte immunoglobulin-like receptors: novel innate receptors for human basophil activation and inhibition
    David E Sloane
    Department of Medicine, Harvard Medical School, Boston, MA, USA
    Blood 104:2832-9. 2004
    ..That LIR3 is profoundly counter-regulatory for both adaptive and innate receptors suggests a broad role in containment of the inflammatory response...
  36. pmc A pH-stabilizing role of voltage-gated proton channels in IgE-mediated activation of human basophils
    Boris Musset
    Department of Molecular Biophysics and Physiology, Rush University Medical Center, Chicago, IL 60612, USA
    Proc Natl Acad Sci U S A 105:11020-5. 2008
    ..Evidently, proton channels are active in basophils during IgE-mediated responses and prevent excessive acidification, which may account for their role in histamine release...
  37. pmc Distinct characteristics of signal transduction events by histamine-releasing factor/translationally controlled tumor protein (HRF/TCTP)-induced priming and activation of human basophils
    Becky M Vonakis
    The Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    Blood 111:1789-96. 2008
    ..This finding is different from IL-3, which also primes basophils for histamine release, but does show phosphorylation of these events. We conclude that priming induced by HRF/TCTP is distinct from that induced by IL-3...
  38. ncbi request reprint Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement
    Hidenori Yokoi
    Department of Medicine, Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, and the Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 121:499-505.e1. 2008
    ..On eosinophils, Siglec-8 engagement induces apoptosis, but its function on mast cells is unknown...
  39. ncbi request reprint Expression of functional cysteinyl leukotriene receptors by human basophils
    Gail M Gauvreau
    Department of Medicine, McMaster University, Hamilton, MD, USA
    J Allergy Clin Immunol 116:80-7. 2005
    ..Receptors for cysLTs are expressed on a variety of peripheral blood leukocytes, but of interest, they are also expressed on cells that synthesize LTC 4 , such as eosinophils and mast cells...
  40. ncbi request reprint Prostaglandin-induced activation of nociceptive neurons via direct interaction with transient receptor potential A1 (TRPA1)
    Thomas E Taylor-Clark
    Johns Hopkins Medical Institutions, Johns Hopkins Asthma and Allergy Center, 3A 44, Baltimore, MD 21224, USA
    Mol Pharmacol 73:274-81. 2008
    ..Taken together, these data suggest a novel mechanism through which reactive prostanoids may activate nociceptive neurons independent of prostaglandin receptors...
  41. ncbi request reprint Diagnostic tests based on human basophils: potentials, pitfalls and perspectives
    Jörg Kleine-Tebbe
    Allergy and Asthma Center Westend, Berlin, Germany
    Int Arch Allergy Immunol 141:79-90. 2006
    ..Diagnostic studies using CD63 or CD203c in hymenoptera, food and drug allergy are critically discussed. Basophil-based tests are indicated for allergy testing in selected cases but should only be performed by experienced laboratories...
  42. pmc Role of chloride channels in bradykinin-induced guinea pig airway vagal C-fibre activation
    Min Goo Lee
    Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Physiol 566:205-12. 2005
    ..We concluded that in vagal afferent C-fibres, BK evokes membrane depolarization and action potential discharge through the additive effects of TRPV1 and Cl- channel activation...
  43. ncbi request reprint Nasal sensory nerve populations responding to histamine and capsaicin
    Thomas E Taylor-Clark
    Department of Medicine, Division of Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 116:1282-8. 2005
    ..Nasal challenge with the C-fiber stimulant capsaicin causes a different set of symptoms than those evoked by histamine, suggesting that these 2 stimuli may activate separate subpopulations of nasal sensory nerves...
  44. ncbi request reprint Acute IL-3 priming up-regulates the stimulus-induced Raf-1-Mek-Erk cascade independently of IL-3-induced activation of Erk
    Natalia Vilariño
    Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA
    J Immunol 175:3006-14. 2005
    ..This type of preactivation can be mimicked by incubation with the Ser-Thr kinase inhibitors, Ro-81-3220, or bisindoylmaleimide II...

Research Grants24

  1. MECHANISMS OF HUMAN BASOPHIL & MAST CELL DESENSITIZATION
    Donald MacGlashan; Fiscal Year: 1992
    ..Phosphorylation of 5'- phosphomonoesterase will be directly examined as will its activity in desensitized basophils and mast cells...
  2. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2003
    ..Since cytokine release appears dependent on mRNA accumulation, initial studies will focus on the mechanisms of mRNA accumulation. ..
  3. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2007
    ..A population of subjects will be examined for this relationship. ..
  4. MECHANISMS OF HUMAN BASOPHIL & MAST CELL DESENSITIZATION
    Donald MacGlashan; Fiscal Year: 1993
    ..Since cytokine release appears dependent on mRNA accumulation, initial studies will focus on the mechanisms of mRNA accumulation...
  5. REGULATION OF SECRETION FROM HUMAN BASOPHILS
    Donald MacGlashan; Fiscal Year: 2009
    ..A population of subjects will be examined for this relationship. ..