Jun O Liu

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
    Fan Pan
    Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    EMBO J 24:2104-13. 2005
  2. pmc Arginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins
    Chao Liu
    State Key Laboratory of Genetic Engineering Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China
    BMC Cell Biol 14:27. 2013
  3. pmc Calmodulin-dependent phosphatase, kinases, and transcriptional corepressors involved in T-cell activation
    Jun O Liu
    Department of Pharmacology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Immunol Rev 228:184-98. 2009
  4. ncbi request reprint Endogenous protein inhibitors of calcineurin
    Jun O Liu
    Department of Pharmacology and Molecular Sciences and Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 311:1103-9. 2003
  5. doi request reprint It takes two binding sites for calcineurin and NFAT to tango
    Jun O Liu
    Department of Pharmacology, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell 33:676-8. 2009
  6. ncbi request reprint The yins of T cell activation
    Jun O Liu
    Department of Pharmacology, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Sci STKE 2005:re1. 2005
  7. pmc Effect of nitroxoline on angiogenesis and growth of human bladder cancer
    Joong Sup Shim
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    J Natl Cancer Inst 102:1855-73. 2010
  8. doi request reprint Fumagillin and fumarranol interact with P. falciparum methionine aminopeptidase 2 and inhibit malaria parasite growth in vitro and in vivo
    Xiaochun Chen
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Chem Biol 16:193-202. 2009
  9. pmc Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization
    KangAe Lee
    Vascular Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 106:17910-5. 2009
  10. pmc Synergistic inhibition of endothelial cell proliferation, tube formation, and sprouting by cyclosporin A and itraconazole
    Benjamin A Nacev
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 6:e24793. 2011

Collaborators

Detail Information

Publications66

  1. pmc Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation
    Fan Pan
    Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    EMBO J 24:2104-13. 2005
    ..CaMKIV-mediated nuclear export of Cabin1 is likely to account for a significant part of the requirement of CaMKIV during human T-cell activation...
  2. pmc Arginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins
    Chao Liu
    State Key Laboratory of Genetic Engineering Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China
    BMC Cell Biol 14:27. 2013
    ..All of them can be conjugated to PE through a ubiquitin-like conjugation system, and be located to autophagosomes...
  3. pmc Calmodulin-dependent phosphatase, kinases, and transcriptional corepressors involved in T-cell activation
    Jun O Liu
    Department of Pharmacology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Immunol Rev 228:184-98. 2009
    ..The crosstalks among these and other signal transducers in T cells form an extensive nonlinear signaling network that dictates the final outcome of the TCR signaling pathway...
  4. ncbi request reprint Endogenous protein inhibitors of calcineurin
    Jun O Liu
    Department of Pharmacology and Molecular Sciences and Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 311:1103-9. 2003
    ..These endogenous calcineurin inhibitors are throwing new light on the function and regulation of calcineurin in a wide variety of cellular processes and cell types...
  5. doi request reprint It takes two binding sites for calcineurin and NFAT to tango
    Jun O Liu
    Department of Pharmacology, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell 33:676-8. 2009
    ..2009) identified the NFAT LxVP motif binding site as the same composite surface formed by the two calcineurin subunits that is recognized by the cyclophilin-CsA and FKBP-FK506 complexes...
  6. ncbi request reprint The yins of T cell activation
    Jun O Liu
    Department of Pharmacology, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Sci STKE 2005:re1. 2005
    ....
  7. pmc Effect of nitroxoline on angiogenesis and growth of human bladder cancer
    Joong Sup Shim
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    J Natl Cancer Inst 102:1855-73. 2010
    ..Type 2 methionine aminopeptidase (MetAP2) protein is likely a molecular target of angiogenesis inhibitors...
  8. doi request reprint Fumagillin and fumarranol interact with P. falciparum methionine aminopeptidase 2 and inhibit malaria parasite growth in vitro and in vivo
    Xiaochun Chen
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Chem Biol 16:193-202. 2009
    ..These findings suggest that PfMetAP2 is a viable target, and fumarranol is a promising lead compound for the development of novel antimalarial agents...
  9. pmc Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization
    KangAe Lee
    Vascular Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 106:17910-5. 2009
    ..These results provide proof of principle that small molecules can inhibit dimerization of HIF-1 and have potent inhibitory effects on tumor growth and vascularization...
  10. pmc Synergistic inhibition of endothelial cell proliferation, tube formation, and sprouting by cyclosporin A and itraconazole
    Benjamin A Nacev
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 6:e24793. 2011
    ..Thus, it is possible to combine existing clinical drugs to synergistically inhibit in vitro models of angiogenesis. This strategy may be useful in pursuing the next generation of antiangiogenesis therapy...
  11. pmc Pyridinylquinazolines selectively inhibit human methionine aminopeptidase-1 in cells
    Feiran Zhang
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    J Med Chem 56:3996-4016. 2013
    ..Subsequently, we demonstrated that 11j, an auxiliary metal-dependent inhibitor, effectively inhibited HsMetAP1 in primary cells. This is the first report that an HsMetAP1-selective inhibitor is effective against its target in cells...
  12. pmc Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression
    Xiaoyi Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 103:18148-53. 2006
    ..Together, these results suggest that MetAP1 plays an important role in G(2)/M phase of the cell cycle and that it may serve as a promising target for the discovery and development of new anticancer agents...
  13. ncbi request reprint Inhibition of angiogenesis by the antifungal drug itraconazole
    Curtis R Chong
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    ACS Chem Biol 2:263-70. 2007
    ..Together, these findings suggest that itraconazole has the potential to serve as an antiangiogenic drug and that lanosterol 14DM is a promising new target for discovering new angiogenesis inhibitors...
  14. pmc Inhibitors of Plasmodium falciparum methionine aminopeptidase 1b possess antimalarial activity
    Xiaochun Chen
    Department of Pharmacology and Molecular Sciences Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 103:14548-53. 2006
    ..These results suggest that PfMetAP1b is a promising target and XC11 is an important lead compound for the development of novel antimalarial drugs...
  15. pmc Digoxin and other cardiac glycosides inhibit HIF-1alpha synthesis and block tumor growth
    Huafeng Zhang
    Vascular Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 105:19579-86. 2008
    ....
  16. pmc Cholesterol trafficking is required for mTOR activation in endothelial cells
    Jing Xu
    Department of Pharmacology and Oncology, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:4764-9. 2010
    ..These observations suggest that mTOR is likely to be involved in sensing membrane sterol concentrations in endothelial cells, and the cholesterol trafficking pathway is a promising target for the discovery of inhibitors of angiogenesis...
  17. doi request reprint Characterization of clioquinol and analogues as novel inhibitors of methionine aminopeptidases from Mycobacterium tuberculosis
    Omonike Olaleye
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Tuberculosis (Edinb) 91:S61-5. 2011
    ..Together, these results revealed a new mode of action of clioquinol and its congeners and validated the therapeutic potential of this pharmacophore for TB chemotherapy...
  18. pmc Calcineurin promotes hypoxia-inducible factor 1alpha expression by dephosphorylating RACK1 and blocking RACK1 dimerization
    Ye V Liu
    Vascular Program, Institute for Cell Engineering, Department of Pharmacology and Molecular Sciences, Mass Spectronomy Proteomics Facility, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 282:37064-73. 2007
    ..These results indicate that intracellular calcium levels can regulate HIF-1alpha expression by modulating calcineurin activity and RACK1 dimerization...
  19. ncbi request reprint Substrate-dependent targeting of eukaryotic translation initiation factor 4A by pateamine A: negation of domain-linker regulation of activity
    Woon Kai Low
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Chem Biol 14:715-27. 2007
    ..Furthermore, binding of PatA is dependent on substrate (RNA and ATP) binding, and the increased activity upon PatA binding is caused by relief of a negative regulatory function of the eIF4A unique domain linker...
  20. pmc Clofazimine inhibits human Kv1.3 potassium channel by perturbing calcium oscillation in T lymphocytes
    Yunzhao R Ren
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 3:e4009. 2008
    ..Together, these results suggest that clofazimine is a promising immunomodulatory drug candidate for treating a variety of autoimmune disorders...
  21. pmc Inhibition of nonsense-mediated mRNA decay by the natural product pateamine A through eukaryotic initiation factor 4AIII
    Yongjun Dang
    Department of Pharmacology, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:23613-21. 2009
    ..Furthermore, we have characterized the mechanisms by which PatA and cycloheximide modulate NMD. Unlike CHX, PatA was found to inhibit NMD by a novel mechanism that is independent of the phosphorylation of Up-frameshift protein 1...
  22. pmc Malaria-infected mice live until at least day 30 after a new monomeric trioxane combined with mefloquine are administered together in a single low oral dose
    Lauren E Woodard
    Department of Chemistry, School of Arts and Sciences, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218 2685, USA
    J Med Chem 52:7458-62. 2009
    ....
  23. pmc RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alpha
    Stefanie Galban
    LCMB, NIA, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224, USA
    Mol Cell Biol 28:93-107. 2008
    ..Conversely, HIF-1alpha expression and translation in response to CoCl(2) were markedly elevated after HuR overexpression. We propose that HuR and PTB jointly upregulate HIF-1alpha translation in response to CoCl(2)...
  24. pmc XPB, a subunit of TFIIH, is a target of the natural product triptolide
    Denis V Titov
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Chem Biol 7:182-8. 2011
    ..These findings also suggest that triptolide can serve as a new molecular probe for studying transcription and, potentially, as a new type of anticancer agent through inhibition of the ATPase activity of XPB...
  25. pmc Substituted oxines inhibit endothelial cell proliferation and angiogenesis
    Shridhar Bhat
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Org Biomol Chem 10:2979-92. 2012
    ..The bioactivity of the second hit, micromolar HUVEC and MetAP2 inhibitor carbamate 22 was improved further with an SAR study culminating in carbamate 24 which is a nanomolar inhibitor of HUVEC and MetAP2...
  26. ncbi request reprint Fumarranol, a rearranged fumagillin analogue that inhibits angiogenesis in vivo
    Jun Lu
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Med Chem 49:5645-8. 2006
    ..It is also active in a mouse model of angiogenesis in vivo. Unlike TNP-470, fumarranol does not covalently bind to MetAP2. Fumarranol may serve as a lead for a new class of angiogenesis inhibitors...
  27. pmc Analogs of N'-hydroxy-N-(4H,5H-naphtho[1,2-d]thiazol-2-yl)methanimidamide inhibit Mycobacterium tuberculosis methionine aminopeptidases
    Shridhar Bhat
    Department of Pharmacology and Molecular Sciences, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Bioorg Med Chem 20:4507-13. 2012
    ..However, the SAR data generated thus far may prove valuable for further tuning of this class of inhibitors as effective anti-tuberculosis agents...
  28. pmc Itraconazole inhibits angiogenesis and tumor growth in non-small cell lung cancer
    Blake T Aftab
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland, USA
    Cancer Res 71:6764-72. 2011
    ..Based on these observations, we have initiated a randomized phase II study comparing the efficacy of standard cytotoxic therapy with or without daily oral itraconazole in patients with recurrent metastatic NSCLC...
  29. ncbi request reprint Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin
    Fan Pan
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nature 445:433-6. 2007
    ..Thus, Carabin is a negative feedback inhibitor of the calcineurin signalling pathway that also mediates crosstalk between calcineurin and Ras...
  30. ncbi request reprint Isolation and identification of eukaryotic initiation factor 4A as a molecular target for the marine natural product Pateamine A
    Woon Kai Low
    Department of Pharmacology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
    Methods Enzymol 431:303-24. 2007
    ..We have attempted to present the methodology as a general technique for the identification of protein targets for small molecules including natural products...
  31. pmc Tricyclic thiazoles are a new class of angiogenesis inhibitors
    Shridhar Bhat
    Department of Pharmacology and Molecular Sciences, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Bioorg Med Chem Lett 23:2733-7. 2013
    ..Thus, 37 and 43 can serve as leads to develop a novel class of antiangiogenic agents...
  32. pmc A calcineurin-independent mechanism of angiogenesis inhibition by a nonimmunosuppressive cyclosporin A analog
    Benjamin A Nacev
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    J Pharmacol Exp Ther 338:466-75. 2011
    ..These results suggest that the inhibition of cyclophilins may play a larger role in the antiangiogenic activity of CsA than previously believed, and that cyclophilins may be potential antiangiogenic drug targets...
  33. pmc Itraconazole side chain analogues: structure-activity relationship studies for inhibition of endothelial cell proliferation, vascular endothelial growth factor receptor 2 (VEGFR2) glycosylation, and hedgehog signaling
    Wei Shi
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, United States
    J Med Chem 54:7363-74. 2011
    ..These results also suggest that modification of the sec-butyl side chain can lead to enhancement of the biological activity of itraconazole...
  34. pmc Inhibition of eukaryotic translation elongation by the antitumor natural product Mycalamide B
    Yongjun Dang
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    RNA 17:1578-88. 2011
    ..Moreover, they display different polysome profiles in vivo. Together, these observations shed new light on the mechanism of inhibition of translation elongation by MycB...
  35. pmc Selective inhibition of HER2-positive breast cancer cells by the HIV protease inhibitor nelfinavir
    Joong Sup Shim
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    J Natl Cancer Inst 104:1576-90. 2012
    ..With few treatment options available, new drug targets specific for HER2-positive breast cancer are needed...
  36. pmc A novel two-stage, transdisciplinary study identifies digoxin as a possible drug for prostate cancer treatment
    Elizabeth A Platz
    Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Cancer Discov 1:68-77. 2011
    ..54, 95% CI 0.37-0.79, P-trend<0.001), had a lower prostate cancer risk. Digoxin was highly potent in inhibiting prostate cancer cell growth in vitro and its use was associated with a 25% lower prostate cancer risk...
  37. pmc The antifungal drug itraconazole inhibits vascular endothelial growth factor receptor 2 (VEGFR2) glycosylation, trafficking, and signaling in endothelial cells
    Benjamin A Nacev
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 286:44045-56. 2011
    ....
  38. pmc Eos mediates Foxp3-dependent gene silencing in CD4+ regulatory T cells
    Fan Pan
    Immunology and Hematopoiesis Division, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Science 325:1142-6. 2009
    ..Silencing of Eos in Tregs abrogates their ability to suppress immune responses and endows them with partial effector function, thus demonstrating the critical role that Eos plays in Treg programming...
  39. pmc Anthracycline chemotherapy inhibits HIF-1 transcriptional activity and tumor-induced mobilization of circulating angiogenic cells
    KangAe Lee
    Vascular Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 106:2353-8. 2009
    ..These results provide a molecular basis for the antiangiogenic effect of anthracycline therapy and have important implications for refining the use of these drugs to treat human cancer more effectively...
  40. ncbi request reprint Kinetic and mutational studies of the number of interacting divalent cations required by bacterial and human methionine aminopeptidases
    Xiaoyi V Hu
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Biochemistry 46:12833-43. 2007
    ..Together, these results suggest that human MetAP1 is distinct from other members of the MetAP superfamily in the number of metal ions employed and likely mechanism of catalysis...
  41. pmc Phase 2 study of pemetrexed and itraconazole as second-line therapy for metastatic nonsquamous non-small-cell lung cancer
    Charles M Rudin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, 1550 Orleans Street, Baltimore, MD 21231, USA
    J Thorac Oncol 8:619-23. 2013
    ..On the basis of these data, we performed an exploratory clinical study, assessing the efficacy of itraconazole with cytotoxic chemotherapy in the treatment of patients with advanced lung cancer...
  42. pmc Novel structurally related compounds reactivate latent HIV-1 in a bcl-2-transduced primary CD4+ T cell model without inducing global T cell activation
    Sifei Xing
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Antimicrob Chemother 67:398-403. 2012
    ..Eradication strategies involve reactivation of this latent reservoir; however, agents that reactivate latent HIV-1 through non-specific T cell activation are toxic...
  43. pmc Disulfiram is a DNA demethylating agent and inhibits prostate cancer cell growth
    Jianqing Lin
    Chemical Therapeutics Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Prostate 71:333-43. 2011
    ..Because DNMT catalysis features attack of cytosine bases by an enzyme thiol group, we tested whether disulfiram (DSF), a thiol-reactive compound with known clinical safety, demonstrated DNMT inhibitory activity...
  44. pmc Malaria-infected mice are cured by a single oral dose of new dimeric trioxane sulfones which are also selectively and powerfully cytotoxic to cancer cells
    Andrew S Rosenthal
    Department of Chemistry, School of Arts and Sciences, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218 2685, USA
    J Med Chem 52:1198-203. 2009
    ..Both dimers 4c and 4b were potently and selectively cytotoxic toward five cancer cell lines...
  45. ncbi request reprint Myocyte enhancer factor 2 mediates calcium-dependent transcription of the interleukin-2 gene in T lymphocytes: a calcium signaling module that is distinct from but collaborates with the nuclear factor of activated T cells (NFAT)
    Fan Pan
    Department of Pharmacology and Molecular Science and Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:14477-80. 2004
    ..These results suggest that MEF2 is required for the transcriptional activation of IL-2 and likely other cytokine genes in response to calcium signaling and may serve as a novel target for development of immunosuppressants...
  46. pmc Small-molecule screening using a human primary cell model of HIV latency identifies compounds that reverse latency without cellular activation
    Hung Chih Yang
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Clin Invest 119:3473-86. 2009
    ..Our study expands the number of classes of latency-reversing therapeutics and demonstrates the utility of this in vitro model for finding strategies to eradicate HIV-1 infection...
  47. pmc High-throughput screen identifies novel inhibitors of cancer biomarker α-methylacyl coenzyme A racemase (AMACR/P504S)
    Brice A P Wilson
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Mol Cancer Ther 10:825-38. 2011
    ..This report shows the first high-throughput screen for the discovery of novel AMACR inhibitors, characterizes the first nonsubstrate-based inhibitors, and validates that AMACR is a viable chemotherapeutic target in vitro...
  48. pmc Efficient drug screening and gene correction for treating liver disease using patient-specific stem cells
    Su Mi Choi
    Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Hepatology 57:2458-68. 2013
    ....
  49. pmc Chemical screen identifies FDA-approved drugs and target pathways that induce precocious pancreatic endocrine differentiation
    Meritxell Rovira
    Department of Surgery, and McKusick Nathans Institute for Genetic Medicine, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 108:19264-9. 2011
    ..Using pharmacological approaches, we have identified and characterized two unique pathways in β-cell differentiation in the zebrafish, including down-regulation of GTP production and retinoic acid biosynthesis...
  50. ncbi request reprint Identification of type 1 inosine monophosphate dehydrogenase as an antiangiogenic drug target
    Curtis R Chong
    Department of Pharmacology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Med Chem 49:2677-80. 2006
    ..Using RNA interference, we found that knockdown of one of the two known isoforms of inosine monophosphate dehydrogenase (IMPDH-1) is sufficient to cause endothelial cell cycle arrest...
  51. pmc Regulation of c-Src nonreceptor tyrosine kinase activity by bengamide A through inhibition of methionine aminopeptidases
    Xiaoyi Hu
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University, School of Medicine, 725 N Wolfe Street Baltimore, MD 21205, USA
    Chem Biol 14:764-74. 2007
    ..Thus, c-Src is a physiologically relevant substrate for MetAPs whose dysfunction is likely to account for the cell-cycle effects of MetAP inhibitors including bengamide A...
  52. pmc Identification of inhibitors of ABCG2 by a bioluminescence imaging-based high-throughput assay
    Yimao Zhang
    Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
    Cancer Res 69:5867-75. 2009
    ..The BLI-based assay is an efficient method to identify new inhibitors of ABCG2. As they were derived from a FDA-approved compound library, many of the inhibitors uncovered in this study are ready for clinical testing...
  53. pmc Identification and validation of protein targets of bioactive small molecules
    Denis V Titov
    Department of Pharmacology, Johns Hopkins University School of Medicine, MD, USA
    Bioorg Med Chem 20:1902-9. 2012
    ..A special emphasis is placed on target validation, including correlation analysis and genetic methods, as this area is often ignored despite its importance...
  54. pmc Methionine aminopeptidases from Mycobacterium tuberculosis as novel antimycobacterial targets
    Omonike Olaleye
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Chem Biol 17:86-97. 2010
    ..Moreover, knockdown of MtMetAP1a, but not MtMetAP1c, resulted in decreased viability of M. tuberculosis. These results suggest that MtMetAP1a is a promising target for developing antituberculosis agents...
  55. ncbi request reprint A clinical drug library screen identifies astemizole as an antimalarial agent
    Curtis R Chong
    Department of Pharmacology and Molecular Sciences The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Chem Biol 2:415-6. 2006
    ..The antihistamine astemizole and its principal human metabolite are promising new inhibitors of chloroquine-sensitive and multidrug-resistant parasites, and they show efficacy in two mouse models of malaria...
  56. pmc The antiherpetic drug acyclovir inhibits HIV replication and selects the V75I reverse transcriptase multidrug resistance mutation
    Moira A McMahon
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 283:31289-93. 2008
    ..Although acyclovir may prove a useful lead for development of new HIV treatments, the selection of resistant mutants raises a cautionary note to the use of acyclovir monotherapy in patients coinfected with HSV and HIV...
  57. ncbi request reprint Eukaryotic initiation factor 2alpha-independent pathway of stress granule induction by the natural product pateamine A
    Yongjun Dang
    Department of Pharmacology and Molecular Sciences, Solomon H Snyder Department of Neuroscience, and Department of Oncology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 281:32870-8. 2006
    ....
  58. ncbi request reprint Identification of pyridinylpyrimidines as inhibitors of human methionine aminopeptidases
    Xiaoyi Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Angew Chem Int Ed Engl 45:3772-5. 2006
  59. ncbi request reprint Regulator of Ras Depalmitoylation and Retrograde Trafficking: A New Hat for FKBP
    Jun O Liu
    Department of Pharmacology, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA Department of Oncology, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Mol Cell 41:131-3. 2011
    ..In this issue of Molecular Cell, Ahearn et al. (2011) identified FKBP12 as a novel regulator of Ras signaling through its modulation of depalmitoylation of H-Ras and its recycling from plasma membrane to the Golgi...
  60. ncbi request reprint Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2
    Aidong Han
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309 0215, USA
    Nature 422:730-4. 2003
    ..Our studies of the ternary Cabin1/MEF2/DNA complex show a general mechanism by which MEF2 recruits transcriptional co-repressor Cabin1 and class II HDACs to specific DNA sites...
  61. pmc Calpain-dependent cleavage of cain/cabin1 activates calcineurin to mediate calcium-triggered cell death
    Min Jung Kim
    Department of Life Science, Kwangju Institute of Science and Technology, 1 Oryong dong, Puk Gu, Kwangju 500 712, Korea
    Proc Natl Acad Sci U S A 99:9870-5. 2002
    ..Taken together, these results indicate that calpain cleaves the calcineurin-binding domain of cain/cabin1 to activate Cn and elicit calcium-triggered cell death...
  62. ncbi request reprint Nuclear export of NF90 to stabilize IL-2 mRNA is mediated by AKT-dependent phosphorylation at Ser647 in response to CD28 costimulation
    Yuan Pei
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, China
    J Immunol 180:222-9. 2008
    ..In vivo and in vitro data support a model in which CD28 costimulation activates AKT to phosphorylate NF90 at Ser647 and phosphorylation triggers NF90 to relocate to the cytoplasm and stabilize IL-2 mRNA...
  63. ncbi request reprint The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation
    Mike Boxem
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    FEBS Lett 576:245-50. 2004
    ..These observations suggest that MAP-2 is essential for germ cell development in C. elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner...
  64. ncbi request reprint Reading dynamic kinase activity in living cells for high-throughput screening
    Michael D Allen
    ACS Chem Biol 1:371-6. 2006
    ....
  65. pmc Crystal structure of calcineurin-cyclophilin-cyclosporin shows common but distinct recognition of immunophilin-drug complexes
    Qing Huai
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Proc Natl Acad Sci U S A 99:12037-42. 2002
    ..The comparison of CyPA-CsA-CN with FKBP-FK506-CN significantly contributes to understanding the molecular basis of regulation of CN activity by the immunophilin-immunosuppressant...
  66. ncbi request reprint Mechanism of recruitment of class II histone deacetylases by myocyte enhancer factor-2
    Aidong Han
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309 0215, USA
    J Mol Biol 345:91-102. 2005
    ..Such specificity provides a basis for selectively disrupting a particular MEF2/co-regulator complex by mutations or small molecules...

Research Grants35

  1. NF-kappaB Signaling Pathway Probed with Natural Products
    Jun Liu; Fiscal Year: 2007
    ....
  2. INHIBITION OF ANGIOGENESIS BY TNP 470 AND OVALICIN
    Jun Liu; Fiscal Year: 2007
    ..The newly identified inhibitors for MetAP1 will be employed to assess the physiological role of MetAP1 in the cell cycle progression in the G2/M phase. ..
  3. PHARMACOLOGY TRAINING GRANT
    Jun Liu; Fiscal Year: 2007
    ....
  4. Discovery and Investigation of Novel Angiogenesis Inhibitors Among Existing Drugs
    Jun O Liu; Fiscal Year: 2010
    ....
  5. SIGNAL TRANSDUCTION BY CALCINEURIN IN T LYMPHOCYTES
    Jun Liu; Fiscal Year: 2003
    ....
  6. INHIBITION OF ANGIOGENESIS BY TNP 470 AND OVALICIN
    Jun Liu; Fiscal Year: 2002
    ..These drug-RGD peptide conjugates may prove to be much more selective and less toxic than TNP-470 and related angiogenesis inhibitors. ..
  7. MECHANISM(S) OF INHIBITION OF ANGIOGENESIS BY FUMAGILLIN, OVALICIN & TNP 470
    Jun Liu; Fiscal Year: 2001
    ....
  8. Structure, Function and Inhibition of Human Methionine Aminopeptidases
    Jun O Liu; Fiscal Year: 2010
    ..The main objective of this application is to explore the type 1 and type 2 human methionine aminopeptidases as targets and their inhibitors as leads for the development of anti-angiogenic and anti-cancer agents. ..