Research Topics
Genomes and Genes | M LaneSummaryAffiliation: Johns Hopkins University Country: USA Publications
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Publications
Adipose development: from stem cell to adipocyteTamara C Otto
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Crit Rev Biochem Mol Biol 40:229-42. 2005..C/EBPalpha, peroxisome proliferator-activated receptor gamma, and adipocyte determination, and differentiation-dependent factor 1 coordinate the expression of genes that create and maintain the adipocyte phenotype...
Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: role of the BMP-4 geneRobert R Bowers
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 103:13022-7. 2006..Together, these studies provide compelling evidence for the participation of BMP-4 in adipocyte lineage determination...- The role of hypothalamic malonyl-CoA in energy homeostasisMichael J Wolfgang
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 281:37265-9. 2006..Together these findings support a role for malonyl-CoA as an intermediary in the control of energy homeostasis... - Regulation of the O-linked beta-N-acetylglucosamine transferase by insulin signalingStephen A Whelan
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
J Biol Chem 283:21411-7. 2008..We conclude that insulin stimulates the tyrosine phosphorylation and activity of OGT... 
Adipogenesis: from stem cell to adipocyteQi Qun Tang
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Annu Rev Biochem 81:715-36. 2012..quot;Activated" C/EBPβ then triggers transcription of peroxisome proliferator-activated receptor-γ (PPARγ) and C/EBPα, which in turn coordinately activate genes whose expression produces the adipocyte phenotype...- From multipotent stem cell to adipocyteM Daniel Lane
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Birth Defects Res A Clin Mol Teratol 73:476-7. 2005 - Effect of glucose and fructose on food intake via malonyl-CoA signaling in the brainM Daniel Lane
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 512 Wood Basic Science Building, 725 N Wolfe Street, Baltimore, MD 21205, United States
Biochem Biophys Res Commun 382:1-5. 2009..The fact that fructose metabolism by the brain increases food intake and obesity risk raises health concerns in view of the large and increasing per capita consumption of high fructose sweeteners, especially by youth... - The biotin connection: Severo Ochoa, Harland Wood, and Feodor LynenM Daniel Lane
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 279:39187-94. 2004 - Regulation of food intake and energy expenditure by hypothalamic malonyl-CoAM D Lane
Department of Biological Chemistry, Johns Hopkins University Medical School, Baltimore, MD 21205, USA
Int J Obes (Lond) 32:S49-54. 2008..CPT1cKO mice exhibit decreased rates of fatty acid oxidation, consistent with their increased susceptibility to diet-induced obesity. We suggest that CPT1c may be a downstream target of malonyl-CoA that regulates energy homeostasis... - Role of malonyl-CoA in the hypothalamic control of food intake and energy expenditureM D Lane
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Biochem Soc Trans 33:1063-7. 2005..Thus malonyl-CoA appears to link the energy-responsive fatty acid synthesis in the hypothalamus to feeding behaviour and peripheral energy expenditure... - Perturbations in O-linked beta-N-acetylglucosamine protein modification cause severe defects in mitotic progression and cytokinesisChad Slawson
Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2185, USA
J Biol Chem 280:32944-56. 2005..These data suggest that dynamic O-GlcNAc processing is a pivotal regulatory component of the cell cycle, controlling cell cycle progression by regulating mitotic phosphorylation, cyclin expression, and cell division... - Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytesJiang Wen Zhang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 101:43-7. 2004..These findings show that both MCE and adipogenesis are dependent on C/EBPbeta... - Elevated nucleocytoplasmic glycosylation by O-GlcNAc results in insulin resistance associated with defects in Akt activation in 3T3-L1 adipocytesKeith Vosseller
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 99:5313-8. 2002..These results suggest that elevation of O-GlcNAc levels attenuate insulin signaling and contribute to the mechanism by which increased flux through the HSP leads to insulin resistance in adipocytes... - Hypothalamic malonyl-coenzyme A and the control of energy balanceMichael J Wolfgang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 512 WBSB, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
Mol Endocrinol 22:2012-20. 2008.... - A role for hypothalamic malonyl-CoA in the control of food intakeZhiyuan Hu
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 280:39681-3. 2005..c.v.-administered C75, a fatty acid synthase inhibitor that increases hypothalamic [malonyl-CoA]. Taken together these findings implicate malonyl-CoA in the hypothalamic regulation of feeding behavior... - BMP-4 treatment of C3H10T1/2 stem cells blocks expression of MMP-3 and MMP-13Tamara C Otto
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 353:1097-104. 2007..The findings indicate that BMP-4-induced down-regulation of MMP-3 and MMP-13 is associated with commitment, but is insufficient to induce adipogenesis... - Role of cdk2 in the sequential phosphorylation/activation of C/EBPbeta during adipocyte differentiationXi Li
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 104:11597-602. 2007..Thus, MAPK and cdk2/cyclinA act sequentially to maintain Thr(188) of C/EBPbeta in the primed phosphorylated state during MCE and thereby progression of terminal differentiation... - Effect of phosphorylation and S-S bond-induced dimerization on DNA binding and transcriptional activation by C/EBPbetaJae Woo Kim
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 104:1800-4. 2007..These findings indicate that dual phosphorylation of C/EBPbeta/LAP caused a conformational change that facilitates S-S bond formation and dimerization, rendering the basic region accessible to the C/EBP regulatory element... - Wnt signaling and adipocyte lineage commitmentRobert R Bowers
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, Maryland, USA
Cell Cycle 7:1191-6. 2008..These and other findings indicate that activation of Wnt signaling is an early event in adipogenesis... - Upstream stimulatory factors regulate the C/EBP alpha gene during differentiation of 3T3-L1 preadipocytesJae Woo Kim
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 354:517-21. 2007..The expression of AP-4 is reciprocally regulated with USF-1 during adipocyte differentiation. These findings suggest that USF-1 and 2 play roles in C/EBPalpha expression, whereas the AP-4 represses it... - Differential effects of central fructose and glucose on hypothalamic malonyl-CoA and food intakeSeung Hun Cha
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 105:16871-5. 2008..These findings explain the paradoxical fructose effect on food intake and lend credence to the malonyl-CoA hypothesis... - Temporal profiling of the adipocyte proteome during differentiation using a five-plex SILAC based strategyHenrik Molina
McKusick Nathans Institute for Genetic Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
J Proteome Res 8:48-58. 2009..This study using a 5-plex SILAC to investigate dynamics illustrates the power of this approach to identify differentially expressed proteins in a temporal fashion... - Role of CREB in transcriptional regulation of CCAAT/enhancer-binding protein beta gene during adipogenesisJiang Wen Zhang
Department of Biological Chemistry and Biochemistry, Cellular and Molecular Biology Program, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 279:4471-8. 2004..The low level of expression of C/EBPbeta and differentiation in CREB(-/-) MEFs appears to be due to up-regulation of other CREB protein family members, i.e. ATF1 and CREM... - Long-term effects of a fatty acid synthase inhibitor on obese mice: food intake, hypothalamic neuropeptides, and UCP3Seung Hun Cha
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 317:301-8. 2004.... - Commitment of C3H10T1/2 pluripotent stem cells to the adipocyte lineageQi-Qun Tang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 101:9607-11. 2004..We interpret the findings as evidence that BMP4 is capable of triggering commitment of pluripotent C3H10T1/2 stem cells to the adipocyte lineage... - Lytic replication-associated protein (RAP) encoded by Kaposi sarcoma-associated herpesvirus causes p21CIP-1-mediated G1 cell cycle arrest through CCAAT/enhancer-binding protein-alphaFrederick Y Wu
Molecular Virology Laboratories, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
Proc Natl Acad Sci U S A 99:10683-8. 2002..Therefore, C/EBPalpha is essential for the p21-mediated inhibition of G(1) to S-phase progression by RAP in KSHV-infected host cells... - Inhibition of hypothalamic fatty acid synthase triggers rapid activation of fatty acid oxidation in skeletal muscleSeung Hun Cha
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 102:14557-62. 2005..These findings clarify the mechanism by which the hypothalamic malonyl-CoA signal is communicated to metabolic systems in skeletal muscle that regulate fatty acid oxidation and energy expenditure... - Effect of the anorectic fatty acid synthase inhibitor C75 on neuronal activity in the hypothalamus and brainstemSu Gao
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 100:5628-33. 2003.... - Regulation of hypothalamic malonyl-CoA by central glucose and leptinMichael J Wolfgang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 104:19285-90. 2007..These studies show that hypothalamic malonyl-CoA responds to the level of circulating glucose and leptin, both of which affect energy homeostasis... - A role for bone morphogenetic protein-4 in adipocyte developmentRobert R Bowers
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Cell Cycle 6:385-9. 2007..A role for the BMP-4 signaling pathway in adipogenesis is discussed... - Convergence of peroxisome proliferator-activated receptor gamma and Foxo1 signaling pathwaysPaul Dowell
Biological Chemistry and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 278:45485-91. 2003..A more general convergence of nuclear hormone receptor and forkhead factor pathways may be important for multiple biological processes and this convergence may be evolutionarily conserved... - CCAAT/enhancer-binding protein beta is required for mitotic clonal expansion during adipogenesisQi Qun Tang
Department of Biological Chemistry and Pediatrics Division of Endocrinology, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 100:850-5. 2003..These findings demonstrate that expression of CEBPbeta is a prerequisite for MCE in the adipocyte-differentiation program... - Hypothalamic malonyl-CoA and CPT1c in the treatment of obesityMichael J Wolfgang
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
FEBS J 278:552-8. 2011..Thus, the brain can directly sense and respond to changes in nutrient availability and composition to affect body weight and adiposity... - Central lactate metabolism suppresses food intake via the hypothalamic AMP kinase/malonyl-CoA signaling pathwaySeung Hun Cha
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 386:212-6. 2009..All downstream effects of hypothalamic lactate are blocked by icv administered oxamate, a potent inhibitor of lactate dehydrogenase, thus verifying the central action of lactate... - Mitotic clonal expansion: a synchronous process required for adipogenesisQi Qun Tang
Department of Biological Chemistry, Division of Endocrinology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 100:44-9. 2003..These results show that MCE is a prerequisite for differentiation of 3T3-L1 preadipocytes into adipocytes... - Effect of a fatty acid synthase inhibitor on food intake and expression of hypothalamic neuropeptidesTeruhiko Shimokawa
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 99:66-71. 2002..In both lean and obese mice, C75 markedly increased expression of melanin-concentrating hormone and its receptor in the hypothalamus... - Hypothalamic malonyl-CoA triggers mitochondrial biogenesis and oxidative gene expression in skeletal muscle: Role of PGC-1alphaSeung Hun Cha
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 103:15410-5. 2006..PGC-1alpha also increased the expression of ERRalpha, PPARalpha, and enzymes that support mitochondrial fatty acid oxidation, ATP synthesis, and thermogenesis, apparently mediated by an increased expression of UCP3... - Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesisQi Qun Tang
Departments of Pediatrics Division of Endocrinology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 102:9766-71. 2005..The delayed transactivation of the C/EBPalpha and PPARgamma genes by C/EBPbeta appears necessary to allow mitotic clonal expansion, which would otherwise be prevented, because C/EBPalpha and PPARgamma are antimitotic... - The brain-specific carnitine palmitoyltransferase-1c regulates energy homeostasisMichael J Wolfgang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 103:7282-7. 2006..CPT1c KO mice also exhibit decreased rates of fatty acid oxidation, which may contribute to their increased susceptibility to diet-induced obesity. These findings indicate that CPT1c is necessary for the regulation of energy homeostasis... - C/EBPalpha reverses the anti-adipogenic effects of the HIV protease inhibitor nelfinavirPaul Dowell
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 327:571-4. 2005..These results suggest that nelfinavir may mediate an anti-adipogenic effect by antagonizing expression of C/EBPalpha... - Control of energy homeostasis: role of enzymes and intermediates of fatty acid metabolism in the central nervous systemMichael J Wolfgang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Annu Rev Nutr 26:23-44. 2006..This review focuses on the regulatory roles of these enzymes and intermediates in the regulation of food intake and energy balance... - Monitoring energy balance: metabolites of fatty acid synthesis as hypothalamic sensorsPaul Dowell
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Annu Rev Biochem 74:515-34. 2005..In the hypothalamus, intermediates in this pathway appear to serve as energy sensors that signal higher brain centers to produce appropriate responses, e.g., altered food intake and energy expenditure... - Regulation of insulin receptor substrate 1 (IRS-1)/AKT kinase-mediated insulin signaling by O-Linked beta-N-acetylglucosamine in 3T3-L1 adipocytesStephen A Whelan
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
J Biol Chem 285:5204-11. 2010..We conclude that one of the steps at which O-GlcNAc contributes to insulin resistance is by inhibiting phosphorylation at the Y(608)XXM PI3K p85 binding motif in IRS-1 and possibly at PDK1 as well... - Hypothalamic malonyl-CoA as a mediator of feeding behaviorZhiyuan Hu
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 100:12624-9. 2003..Together these findings suggest that level of hypothalamic malonyl-CoA, which depends on the relative activities of acetyl-CoA carboxylase and FAS, is an indicator of energy status and mediates feeding behavior... - Differential effects of a centrally acting fatty acid synthase inhibitor in lean and obese miceMonica V Kumar
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 99:1921-5. 2002.... - Cell cycle arrest by Kaposi's sarcoma-associated herpesvirus replication-associated protein is mediated at both the transcriptional and posttranslational levels by binding to CCAAT/enhancer-binding protein alpha and p21(CIP-1)Frederick Y Wu
Molecular Virology Laboratories, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231-1000, USA
J Virol 77:8893-914. 2003..Finally, we found that the KSHV lytic cycle could not be triggered by either synchronizing KSHV latently infected PEL cells in G(1) phase or inducing p21 in a C/EBPalpha-independent process... - Effect of centrally administered C75, a fatty acid synthase inhibitor, on ghrelin secretion and its downstream effectsZhiyuan Hu
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 102:3972-7. 2005..We propose a model in which ghrelin secretion plays an intermediary role between malonyl-CoA, the substrate of fatty acid synthase, and the neural circuitry regulating energy homeostasis... - Brain-specific carnitine palmitoyl-transferase-1c: role in CNS fatty acid metabolism, food intake, and body weightMichael J Wolfgang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Neurochem 105:1550-9. 2008..Heterozygous mice display an intermediate phenotype. These findings provide further evidence that CPT1c plays a role in maintaining energy homeostasis, but not through altered fatty acid oxidation... - Localization and effect of ectopic expression of CPT1c in CNS feeding centersYun Dai
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St 512 WBSB, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 359:469-74. 2007.... - Brain fatty acid synthase activates PPARalpha to maintain energy homeostasisManu V Chakravarthy
Department of Internal Medicine, Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Clin Invest 117:2539-52. 2007..Thus, 2 diametrically opposed proteins, FAS (induced by feeding) and PPARalpha (induced by starvation), unexpectedly form an integrative sensory module in the central nervous system to orchestrate energy balance... - Effect of serum on the down-regulation of CHOP-10 during differentiation of 3T3-L1 preadipocytesHaiyan Huang
Key Laboratory of Molecular Medicine, Ministry of Education, Fu Dan University, Shanghai 200032, People s Republic of China
Biochem Biophys Res Commun 338:1185-8. 2005..It appears that a factor(s) present in FBS is required to affect the down-regulation of CHOP-10 necessary for successful terminal differentiation... - Nuclear factor 1 regulates adipose tissue-specific expression in the mouse GLUT4 geneShinji Miura
Division of Clinical Nutrition, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan
Biochem Biophys Res Commun 325:812-8. 2004..We propose a model that explains the requirement for both the ASE and the NF1-binding site for expression of GLUT4 in WAT... - Regulatory sequence elements of mouse GLUT4 gene expression in adipose tissuesShinji Miura
Division of Clinical Nutrition, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan
Biochem Biophys Res Commun 312:277-84. 2003..High-fat diet feeding down-regulated GLUT4 minigene mRNA in -701 transgenic mice, but not in -551 transgenic mice, indicating that HFRE is located within 150 bp between bases -701 and -551 of the GLUT4 gene and is distinct from ASE...
Research Grants
- HORMONAL CONTROL OF ADIPOSE GENE EXPRESSIONM Lane; Fiscal Year: 2007..The 32kDa CUP/AP-2alpha- interacting protein, p32, previously identified in our lab, will be characterized and its role determined. ..
- HORMONAL CONTROL OF ADIPOSE GENE EXPRESSIONM Lane; Fiscal Year: 2002..g., the Myc/Zif and Sp1 sites) that regulate the C/EBPalpha gene. how C/EBPalpha (and adipocyte genes it controls) is hormonally regulated in the differentiated adipocyte notably in insulin and cAMP. ..
- REGULATION OF LIPOGENESISM Lane; Fiscal Year: 2001..for derepression and repression of the SCD1 and SCD2 genes. ..
- HORMONAL CONTROL OF ADIPOSE GENE EXPRESSIONM Lane; Fiscal Year: 1993..We have obtained compelling evidence that C/EBPalpha serves an essential role in the coordinate activation of a group of adipose-specific genes during adipose conversion...
- INSULIN AND ADIPOSE CONVERSIONM Lane; Fiscal Year: 1991..e. whether by altering gene transcription or by altering the rate of specific mRNA turnover. The molecular mechanisms by which this regulation is exerted will be investigated...
- FACTORS AND GENES RESPONSIBLE FOR ADIPOCYTE COMMITMENTM Lane; Fiscal Year: 2006..abstract_text> ..
- Antiadipogenic Influence of HIV Protease InhibitorsM Lane; Fiscal Year: 2005..The long term objective of the proposed research is to facilitate the design of anti-HIV therapeutics that lack lipoatrophic potential. ..
- REGULATION OF LIPOGENESIS AND VLDL SYNTHESIS/ASSEMBLYM Lane; Fiscal Year: 1993..Positive feed-forward" control will also be investigated. * the molecular basis for tissue-specific expression and regulation of the SCD1 and SCD2 genes...