Gyanu Lamichhane

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc The Mycobacterium tuberculosis protein LdtMt2 is a nonclassical transpeptidase required for virulence and resistance to amoxicillin
    Radhika Gupta
    Department of Medicine, Johns Hopkins University, Baltimore, Maryland, USA
    Nat Med 16:466-9. 2010
  2. pmc Protective efficacy of BCG overexpressing an L,D-transpeptidase against M. tuberculosis infection
    Scott T Nolan
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 5:e13773. 2010
  3. ncbi request reprint Definition and annotation of (myco)bacterial non-coding RNA
    Gyanu Lamichhane
    Johns Hopkins University School of Medicine, Center for TB Research, 1550 Orleans St, Baltimore, MD 21231, USA
    Tuberculosis (Edinb) 93:26-9. 2013
  4. pmc Essential metabolites of Mycobacterium tuberculosis and their mimics
    Gyanu Lamichhane
    Johns Hopkins School of Medicine, Department of Medicine, Baltimore, Maryland, USA
    MBio 2:e00301-10. 2011
  5. pmc Role of the dosR-dosS two-component regulatory system in Mycobacterium tuberculosis virulence in three animal models
    Paul J Converse
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Infect Immun 77:1230-7. 2009
  6. ncbi request reprint Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice
    Sanjay K Jain
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    J Infect Dis 195:1634-42. 2007
  7. doi request reprint Murine model to study the invasion and survival of Mycobacterium tuberculosis in the central nervous system
    Nicholas A Be
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Infect Dis 198:1520-8. 2008
  8. ncbi request reprint Deletion of a Mycobacterium tuberculosis proteasomal ATPase homologue gene produces a slow-growing strain that persists in host tissues
    Gyanu Lamichhane
    Center for Tuberculosis Research, Department of Medicine, Johns Hopkins School of Medicine, Johns Hopkins University, Baltimore, MD 21231 1001, USA
    J Infect Dis 194:1233-40. 2006
  9. pmc The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models
    Paul J Converse
    Department of Medicine, Center for Tuberculosis Research, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 5:e10289. 2010
  10. pmc Methionine aminopeptidases from Mycobacterium tuberculosis as novel antimycobacterial targets
    Omonike Olaleye
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Chem Biol 17:86-97. 2010

Collaborators

Detail Information

Publications20

  1. pmc The Mycobacterium tuberculosis protein LdtMt2 is a nonclassical transpeptidase required for virulence and resistance to amoxicillin
    Radhika Gupta
    Department of Medicine, Johns Hopkins University, Baltimore, Maryland, USA
    Nat Med 16:466-9. 2010
    ..tuberculosis. Our study suggests that a combination of L,D-transpeptidase and beta-lactamase inhibitors could effectively target persisting bacilli during the chronic phase of tuberculosis...
  2. pmc Protective efficacy of BCG overexpressing an L,D-transpeptidase against M. tuberculosis infection
    Scott T Nolan
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 5:e13773. 2010
    ..New vaccines are desperately needed to decrease the worldwide burden of TB...
  3. ncbi request reprint Definition and annotation of (myco)bacterial non-coding RNA
    Gyanu Lamichhane
    Johns Hopkins University School of Medicine, Center for TB Research, 1550 Orleans St, Baltimore, MD 21231, USA
    Tuberculosis (Edinb) 93:26-9. 2013
    ..We are providing recommendations for this systematic annotation that we hope will be adopted by the mycobacterial research community. These may also serve as templates for annotation of non-coding RNAs in other bacteria...
  4. pmc Essential metabolites of Mycobacterium tuberculosis and their mimics
    Gyanu Lamichhane
    Johns Hopkins School of Medicine, Department of Medicine, Baltimore, Maryland, USA
    MBio 2:e00301-10. 2011
    ..tuberculosis, inhibited the growth of this organism at micromolar concentrations...
  5. pmc Role of the dosR-dosS two-component regulatory system in Mycobacterium tuberculosis virulence in three animal models
    Paul J Converse
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Infect Immun 77:1230-7. 2009
    ..Our analyses reveal that the dosR and dosS genes are required for full virulence and that there may be differences in the patterns of attenuation of this mutant between the animal models studied...
  6. ncbi request reprint Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice
    Sanjay K Jain
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    J Infect Dis 195:1634-42. 2007
    ..However, unlike mice, M. tuberculosis-infected guinea pigs form caseating granulomas, which may simulate human disease more closely...
  7. doi request reprint Murine model to study the invasion and survival of Mycobacterium tuberculosis in the central nervous system
    Nicholas A Be
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Infect Dis 198:1520-8. 2008
    ..It develops after hematogenous dissemination and subsequent invasion of the CNS by Mycobacterium tuberculosis. The microbial determinants involved in CNS disease are poorly characterized...
  8. ncbi request reprint Deletion of a Mycobacterium tuberculosis proteasomal ATPase homologue gene produces a slow-growing strain that persists in host tissues
    Gyanu Lamichhane
    Center for Tuberculosis Research, Department of Medicine, Johns Hopkins School of Medicine, Johns Hopkins University, Baltimore, MD 21231 1001, USA
    J Infect Dis 194:1233-40. 2006
    ..Thus, loss of MT2175 slows both in vitro and in vivo growth rates and compromises the lethality of M. tuberculosis in mice but has a minimal impact on the organism's ability to persist in host tissues...
  9. pmc The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models
    Paul J Converse
    Department of Medicine, Center for Tuberculosis Research, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 5:e10289. 2010
    ..tuberculosis mutants might be reduced due to multiple in vitro passages, and that virulence might be augmented by passage of these strains through mice before quantitative virulence testing in the mouse or guinea pig aerosol models...
  10. pmc Methionine aminopeptidases from Mycobacterium tuberculosis as novel antimycobacterial targets
    Omonike Olaleye
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Chem Biol 17:86-97. 2010
    ..Moreover, knockdown of MtMetAP1a, but not MtMetAP1c, resulted in decreased viability of M. tuberculosis. These results suggest that MtMetAP1a is a promising target for developing antituberculosis agents...
  11. ncbi request reprint Nonclassical transpeptidases of Mycobacterium tuberculosis alter cell size, morphology, the cytosolic matrix, protein localization, virulence, and resistance to β-lactams
    Maia K Schoonmaker
    Taskforce To Study Resistance Emergence and Antibiotic Discovery Technology, Johns Hopkins University, Baltimore, Maryland, USA
    J Bacteriol 196:1394-402. 2014
    ..tuberculosis to amoxicillin-clavulanate and vancomycin...
  12. ncbi request reprint Cyclic AMP intoxication of macrophages by a Mycobacterium tuberculosis adenylate cyclase
    Nisheeth Agarwal
    Department of Medicine, Johns Hopkins School of Medicine, CRB2, Room 1 08, 1550 Orleans Street, Baltimore, Maryland 21231 1044, USA
    Nature 460:98-102. 2009
    ..Direct intoxication of host cells by bacterial-derived cAMP may enable M. tuberculosis to modify both its intracellular and tissue environments to facilitate its long-term survival...
  13. pmc Targeting the cell wall of Mycobacterium tuberculosis: structure and mechanism of L,D-transpeptidase 2
    Sabri B Erdemli
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Structure 20:2103-15. 2012
    ..Together, this information provides vital insights to facilitate development of drugs targeting this validated yet unexploited enzyme...
  14. pmc A screen for non-coding RNA in Mycobacterium tuberculosis reveals a cAMP-responsive RNA that is expressed during infection
    Shaaretha Pelly
    Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Gene 500:85-92. 2012
    ..We report that ncrMT1302 is expressed in Mtb residing in the lungs of mice during an active infection...
  15. pmc Designer arrays for defined mutant analysis to detect genes essential for survival of Mycobacterium tuberculosis in mouse lungs
    Gyanu Lamichhane
    Center for Tuberculosis Research, Department of Medicine, Division of Infectious Diseases, Johns Hopkins School of Medicine, Baltimore, MD 21231 1001, USA
    Infect Immun 73:2533-40. 2005
    ..Of the 31 genes reported here, 17 have not been previously described to be involved in in vivo growth and survival of M. tuberculosis...
  16. pmc Bacterial thymidine kinase as a non-invasive imaging reporter for Mycobacterium tuberculosis in live animals
    Stephanie L Davis
    Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    PLoS ONE 4:e6297. 2009
    ..Our goal was to use this reporter and develop non-invasive methods to detect and localize Mycobacterium tuberculosis...
  17. pmc Dormancy phenotype displayed by extracellular Mycobacterium tuberculosis within artificial granulomas in mice
    Petros C Karakousis
    Dept of Medicine, Center for Tuberculosis Research, Johns Hopkins University School of Medicine, 1503 E Jefferson St, Rm 112, Baltimore, MD 21231, USA
    J Exp Med 200:647-57. 2004
    ..Interestingly, the dormancy phenotype of extracellular M. tuberculosis within host granulomas appears to be immune mediated and interferon-gamma dependent...
  18. ncbi request reprint Mycobacterium tuberculosis invasion and traversal across an in vitro human blood-brain barrier as a pathogenic mechanism for central nervous system tuberculosis
    Sanjay K Jain
    Division of Pediatric Infectious Diseases and Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    J Infect Dis 193:1287-95. 2006
    ..However, the mechanism(s) involved in this process are poorly understood...
  19. pmc A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis
    Gyanu Lamichhane
    Center for Tuberculosis Research, Department of Medicine, The Johns Hopkins University School of Medicine, 424 North Bond Street, Baltimore, MD 21231 1001, USA
    Proc Natl Acad Sci U S A 100:7213-8. 2003
    ..At subsaturation levels of mutagenesis with a random transposon such as Himar1, this approach permits a statistical prediction of both the proportion and identities of essential genes of sequenced genomes...