Robert E Jensen

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Mmm2p, a mitochondrial outer membrane protein required for yeast mitochondrial shape and maintenance of mtDNA nucleoids
    Matthew J Youngman
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    J Cell Biol 164:677-88. 2004
  2. pmc Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape
    H Sesaki
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 147:699-706. 1999
  3. pmc Mmm1p, a mitochondrial outer membrane protein, is connected to mitochondrial DNA (mtDNA) nucleoids and required for mtDNA stability
    A E Hobbs
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 152:401-10. 2001
  4. ncbi Mitochondrial building blocks
    Robert E Jensen
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Trends Cell Biol 14:215-8. 2004
  5. ncbi Protein import into and across the mitochondrial inner membrane: role of the TIM23 and TIM22 translocons
    Robert E Jensen
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Biochim Biophys Acta 1592:25-34. 2002
  6. ncbi Yeast mitochondrial dynamics: fusion, division, segregation, and shape
    R E Jensen
    Department of Cell Biology and Anatomy, Biophysics 100, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Microsc Res Tech 51:573-83. 2000
  7. ncbi Control of mitochondrial shape
    Robert E Jensen
    Department of Cell Biology, Johns Hopkins Medical School, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Curr Opin Cell Biol 17:384-8. 2005
  8. ncbi Protein translocation: is Hsp70 pulling my chain?
    R E Jensen
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Curr Biol 9:R779-82. 1999
  9. ncbi Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion
    Hiromi Sesaki
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    J Biol Chem 279:28298-303. 2004
  10. ncbi Cells lacking Pcp1p/Ugo2p, a rhomboid-like protease required for Mgm1p processing, lose mtDNA and mitochondrial structure in a Dnm1p-dependent manner, but remain competent for mitochondrial fusion
    Hiromi Sesaki
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 308:276-83. 2003

Collaborators

  • Hiromi Sesaki
  • Cory D Dunn
  • Keith Gull
  • Eva Gluenz
  • Arthur E Johnson
  • Shawn M Burgess
  • Steven M Claypool
  • Terry K Smith
  • Carolyn Machamer
  • Nikolaus Pfanner
  • Toshiya Endo
  • Michael Yaffe
  • Rosemary Stuart
  • Peter Rehling
  • Kaye N Truscott
  • Kara L Cerveny
  • Nathan N Alder
  • Paul T Englund
  • Megan L Povelones
  • Matthew J Youngman
  • Jianyang Wang
  • Yasushi Tamura
  • Alyson E Aiken Hobbs
  • April M Clayton
  • Arnab Roy Chowdhury
  • Lesley A Kane
  • Koji Yamano
  • O Kerscher
  • Pablo M V Peixoto
  • Chris Meisinger
  • Alison J Davis
  • Valerie Everard-Gigot
  • Bernard Guiard
  • Sergey M Grigoriev
  • K R Ryan
  • Peter Kovermann
  • Calvin Tiengwe
  • K L Cerveny
  • A E Hobbs
  • J M McCaffery
  • Miho Iijima
  • Ouma Onguka
  • Jennifer L Guler
  • Rahul Bakshi
  • Gökhan Tolun
  • Valeria Pappas-Brown
  • Theresa A Shapiro
  • A J Davis
  • Gokben Yildirir
  • Beiyu Liu
  • Jack D Griffith
  • N B Sepuri
  • Jennifer E Van Eyk
  • M Srinivasan
  • Jennifer Sutherland
  • Ashley I Buhring
  • Yoh ichi Yatsukawa
  • Masatoshi Esaki
  • Nils Wiedemann
  • Teresa J Roy
  • Agnieszka Chacinska
  • Maria Luisa Campo
  • Seth L Studer
  • Diana Stojanovski
  • Sylvia Pfannschmidt
  • Thomas Becker
  • Montaña Flores
  • Michael Rissler
  • Fernando Graña
  • Dusanka Milenkovic
  • Zhongyan Zhang
  • J Holder
  • Susanne Brunner
  • Brigid M Dolan
  • R S Leung
  • Maithreyan Srinivasan
  • Kathleen W Kinnally
  • Richard Wagner
  • Hanne Muller
  • Naresh B Sepuri
  • S Garrett
  • M M Menold

Detail Information

Publications45

  1. pmc Mmm2p, a mitochondrial outer membrane protein required for yeast mitochondrial shape and maintenance of mtDNA nucleoids
    Matthew J Youngman
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    J Cell Biol 164:677-88. 2004
    ..We speculate that Mmm2p and Mmm1p are components of independent machinery, whose dynamic interactions are required to maintain mitochondrial shape and mtDNA structure...
  2. pmc Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape
    H Sesaki
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 147:699-706. 1999
    ..Our results thus suggest that mitochondrial shape is normally controlled by a balance between division and fusion which requires Dnm1p and Fzo1p, respectively...
  3. pmc Mmm1p, a mitochondrial outer membrane protein, is connected to mitochondrial DNA (mtDNA) nucleoids and required for mtDNA stability
    A E Hobbs
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 152:401-10. 2001
    ..We propose that Mmm1p is part of a connection between the mitochondrial outer and inner membranes, anchoring mitochondrial DNA nucleoids in the matrix...
  4. ncbi Mitochondrial building blocks
    Robert E Jensen
    Department of Cell Biology, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Trends Cell Biol 14:215-8. 2004
    ..Mootha et al. examined mitochondria from mouse brain, heart, kidney and liver cells, finding that a surprising fraction of the proteins are expressed in only a subset of tissues...
  5. ncbi Protein import into and across the mitochondrial inner membrane: role of the TIM23 and TIM22 translocons
    Robert E Jensen
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Biochim Biophys Acta 1592:25-34. 2002
    ..In addition, the core components of both the TIM23 and TIM22 translocons have been shown to form aqueous pores in the mitochondrial IM. In this review, we summarize what is known about import of proteins across the mitochondrial IM...
  6. ncbi Yeast mitochondrial dynamics: fusion, division, segregation, and shape
    R E Jensen
    Department of Cell Biology and Anatomy, Biophysics 100, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Microsc Res Tech 51:573-83. 2000
    ..Below we summarize our current understanding of the molecules known to be required for yeast mitochondrial dynamics...
  7. ncbi Control of mitochondrial shape
    Robert E Jensen
    Department of Cell Biology, Johns Hopkins Medical School, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Curr Opin Cell Biol 17:384-8. 2005
    ..Complicating matters, a recent study raises the possibility that one or more of these shape-forming proteins plays a direct role in the import and assembly of mitochondrial proteins synthesized in the cytosol...
  8. ncbi Protein translocation: is Hsp70 pulling my chain?
    R E Jensen
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Curr Biol 9:R779-82. 1999
    ..Recent experiments aimed at distinguishing between two models for Hsp70 function appear to reach opposite conclusions...
  9. ncbi Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion
    Hiromi Sesaki
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    J Biol Chem 279:28298-303. 2004
    ..Our data indicate that distinct regions of Ugo1p bind directly to Fzo1p and Mgm1p and thereby link these two GTPases during mitochondrial fusion...
  10. ncbi Cells lacking Pcp1p/Ugo2p, a rhomboid-like protease required for Mgm1p processing, lose mtDNA and mitochondrial structure in a Dnm1p-dependent manner, but remain competent for mitochondrial fusion
    Hiromi Sesaki
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 308:276-83. 2003
    ..However, the processing of Mgm1p is not strictly required for mitochondrial fusion, indicating that the 100 kDa form is sufficient to promote fusion...
  11. pmc Role for two conserved intermembrane space proteins, Ups1p and Ups2p, [corrected] in intra-mitochondrial phospholipid trafficking
    Yasushi Tamura
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 287:15205-18. 2012
    ..Our results suggest that Ups proteins and Mdm31p play important roles in phospholipid biosynthesis in mitochondria. Ups proteins may function in phospholipid trafficking between the outer and inner mitochondrial membranes...
  12. ncbi Yeast mitochondrial division and distribution require the cortical num1 protein
    Kara L Cerveny
    Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Dev Cell 12:363-75. 2007
    ..Thus, our studies have revealed an additional role for Dnm1p in mitochondrial transmission through its interaction with Num1p, thereby providing a link between mitochondrial division and inheritance...
  13. pmc Mgr3p and Mgr1p are adaptors for the mitochondrial i-AAA protease complex
    Cory D Dunn
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 19:5387-97. 2008
    ..We speculate that Mgr3p and Mgr1p function in an adaptor complex that targets substrates to the i-AAA protease for degradation...
  14. pmc Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA
    Cory D Dunn
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Genetics 165:35-45. 2003
    ..Our results suggest that increased amounts of Cct6p, Ssb1p, Icy1p, Tip41p, and Pbp1p help overcome the problems resulting from a defect in protein import...
  15. pmc Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane
    Hiromi Sesaki
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Biol Cell 14:2342-56. 2003
    ..Our results raise the possibility that Mgm1p regulates fusion of the mitochondrial outer membrane through its interactions with Fzo1p and Ugo1p...
  16. pmc Ups1p, a conserved intermembrane space protein, regulates mitochondrial shape and alternative topogenesis of Mgm1p
    Hiromi Sesaki
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biol 173:651-8. 2006
    ..Moreover, the human homologue of Ups1p, PRELI, can fully replace Ups1p in yeast cells. Together, our findings provide a conserved mechanism for the alternative topogenesis of Mgm1p and control of mitochondrial morphology...
  17. doi Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1
    Megan L Povelones
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Mol Microbiol 87:713-29. 2013
    ..At later times following TbLOK1 depletion, kDNA was lost and a more drastic alteration in mitochondrial structure was found. Our results demonstrate the close relationship between organelle structure and function in trypanosomes...
  18. pmc Phosphorylation of the F(1)F(o) ATP synthase beta subunit: functional and structural consequences assessed in a model system
    Lesley A Kane
    Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD, USA
    Circ Res 106:504-13. 2010
    ..The role of these phosphorylations is unknown...
  19. pmc The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria
    Kara L Cerveny
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Biol Cell 14:4126-39. 2003
    ..Furthermore, our results indicate that Net2p is required for proper assembly of the mitochondrial fission components to regulate organelle division...
  20. pmc A genomewide screen for petite-negative yeast strains yields a new subunit of the i-AAA protease complex
    Cory D Dunn
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 17:213-26. 2006
    ..Our results highlight the importance of the i-AAA complex and proteolysis at the inner membrane in cells lacking mitochondrial DNA...
  21. pmc SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import
    K R Ryan
    Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    Mol Biol Cell 5:529-38. 1994
    ..Our observations raise the possibility that Sms1p and Mas6p act together to translocate proteins across the inner membrane...
  22. pmc Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane
    O Kerscher
    Department of Cell Biology and Anatomy, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Mol Biol Cell 11:103-16. 2000
    ..We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane...
  23. pmc Division of mitochondria requires a novel DMN1-interacting protein, Net2p
    K L Cerveny
    Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Biol Cell 12:309-21. 2001
    ..Fluorescence and immunoelectron microscopy shows that Dnm1p and Net2p preferentially colocalize at constriction sites along mitochondrial tubules. Our results suggest that Net2p is a new component of the mitochondrial division machinery...
  24. pmc TbPIF8, a Trypanosoma brucei protein related to the yeast Pif1 helicase, is essential for cell viability and mitochondrial genome maintenance
    Jianyang Wang
    Departments of Biological Chemistry Cell Biology, Johns Hopkins Medical School, Baltimore, MD 21205, USA
    Mol Microbiol 83:471-85. 2012
    ..Although we do not yet know its exact function, we conclude that TbPIF8 is essential for cell viability and is important for maintenance of kDNA...
  25. pmc Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria
    A J Davis
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 150:1271-82. 2000
    ..The positive charges are not required for interaction with the Tim9p-Tim10p complex, but are essential for cross-linking of Tim23p to components of the inner membrane insertion machinery, including Tim54p, Tim22p, and Tim12p...
  26. pmc The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane
    O Kerscher
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 139:1663-75. 1997
    ....
  27. pmc MMM1 encodes a mitochondrial outer membrane protein essential for establishing and maintaining the structure of yeast mitochondria
    S M Burgess
    Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Cell Biol 126:1375-91. 1994
    ..We propose that Mmm1p maintains mitochondria in an elongated shape by attaching the mitochondrion to an external framework, such as the cytoskeleton...
  28. pmc UGO1 encodes an outer membrane protein required for mitochondrial fusion
    H Sesaki
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 152:1123-34. 2001
    ..Our results suggest that Ugo1p is a new outer membrane component of the mitochondrial fusion machinery...
  29. pmc Characterization of the mitochondrial inner membrane translocase complex: the Tim23p hydrophobic domain interacts with Tim17p but not with other Tim23p molecules
    K R Ryan
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell Biol 18:178-87. 1998
    ..In contrast, Tim23Cp cannot be coimmunoprecipitated with Tim23p, raising the possibility that the hydrophobic domain of Tim23p does not interact with other Tim23 molecules...
  30. doi Network news: the replication of kinetoplast DNA
    Robert E Jensen
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Annu Rev Microbiol 66:473-91. 2012
    ....
  31. pmc Depletion of mitochondrial acyl carrier protein in bloodstream-form Trypanosoma brucei causes a kinetoplast segregation defect
    April M Clayton
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Eukaryot Cell 10:286-92. 2011
    ..We further speculate that this compositional change affects TAC function, and thus kDNA segregation...
  32. pmc The killing of African trypanosomes by ethidium bromide
    Arnab Roy Chowdhury
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, Maryland, United States of America
    PLoS Pathog 6:e1001226. 2010
    ....
  33. pmc What happens when Trypanosoma brucei leaves Africa
    Robert E Jensen
    Department of Cell Biology, Johns Hopkins Medical School, Baltimore, MD 21205, USA
    Trends Parasitol 24:428-31. 2008
    ..They concluded that these parasites, which resemble yeast petite mutants, are T. brucei sub-species, which have evolved recently through changes in mitochondrial DNA...
  34. doi Fluorescence mapping of mitochondrial TIM23 complex reveals a water-facing, substrate-interacting helix surface
    Nathan N Alder
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, TX 77843 1114, USA
    Cell 134:439-50. 2008
    ..This approach has therefore provided an unprecedented view of a translocon channel structure in an intact, fully operational, membrane-embedded complex...
  35. ncbi Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import
    Koji Yamano
    Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Biol Chem 283:3799-807. 2008
    ..Therefore Tom20 and Tom22 are apparently involved in the same step or sequential steps along the same pathway of targeting signal recognition in import...
  36. pmc Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits
    Nathan N Alder
    Department of Molecular and Cellular Medicine, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    Mol Biol Cell 19:159-70. 2008
    ..These structural changes reveal that the macromolecular arrangement within the TIM23 complex is dynamic and varies with the physiological state of the mitochondrion...
  37. ncbi Control of mitochondrial protein import by pH
    Sergey M Grigoriev
    Division of Basic Sciences, New York University College of Dentistry, New York, NY 10010, USA
    FEBS Lett 553:163-6. 2003
    ..The pattern of the pH dependences of protein import and channel properties suggests TIM23 open probability can limit import of nuclear-encoded proteins into the matrix of yeast mitochondria...
  38. pmc Functional analysis of subunit e of the F1Fo-ATP synthase of the yeast Saccharomyces cerevisiae: importance of the N-terminal membrane anchor region
    Valerie Everard-Gigot
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    Eukaryot Cell 4:346-55. 2005
    ..Finally, we propose a model to explain how Su e supports the assembly of the ATP synthase dimers-oligomers in the mitochondrial membrane...
  39. ncbi Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel
    Peter Kovermann
    Biophysik, Universitat Osnabruck, FB Biologie Chemie, D 49034 Osnabrück, Germany
    Mol Cell 9:363-73. 2002
    ..Thus, a protein insertion complex can combine three essential functions, signal recognition, channel formation, and energy transduction, in one central component...
  40. ncbi Ahead of the curve: mitochondrial fusion and phospholipase D
    Robert E Jensen
    Nat Cell Biol 8:1215-7. 2006
  41. pmc The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import
    Alison J Davis
    Department of Molecular and Cellular Medicine, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    Mol Biol Cell 18:475-86. 2007
    ....
  42. pmc The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria
    Chris Meisinger
    Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, Freiburg, Germany
    EMBO J 26:2229-39. 2007
    ..We conclude that assembly of mitochondrial beta-barrel proteins represents a major function of the morphology proteins Mdm12/Mmm1...
  43. ncbi Awaking TIM22, a dynamic ligand-gated channel for protein insertion in the mitochondrial inner membrane
    Pablo M V Peixoto
    Department of Biochemistry and Molecular Biology, University of Extremadura, 10071 Caceres, Spain
    J Biol Chem 282:18694-701. 2007
    ..As insertion progressed and initial interaction with the signal peptide faded, the channel would close, sustaining its role as a shunt that places trapped proteins into the membrane...
  44. ncbi Regulation of mitochondrial fusion and division
    Kara L Cerveny
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Trends Cell Biol 17:563-9. 2007
    ..In this review, we discuss how mitochondrial dynamics are controlled and how these events are coordinated with cell growth, mitosis, apoptosis and human diseases...
  45. ncbi Barreling through the membrane
    Arthur E Johnson
    Nat Struct Mol Biol 11:113-4. 2004