Kalina Hristova

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc A look at arginine in membranes
    Kalina Hristova
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Membr Biol 239:49-56. 2011
  2. doi request reprint Measuring the energetics of membrane protein dimerization in mammalian membranes
    Lirong Chen
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Am Chem Soc 132:3628-35. 2010
  3. ncbi request reprint Transmembrane helix heterodimerization in lipid bilayers: probing the energetics behind autosomal dominant growth disorders
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore MD 21218, USA
    J Mol Biol 358:1-7. 2006
  4. pmc Effect of the G375C and G346E achondroplasia mutations on FGFR3 activation
    Lijuan He
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 7:e34808. 2012
  5. pmc Energetics of ErbB1 transmembrane domain dimerization in lipid bilayers
    Lirong Chen
    Department of Materials Science and Engineering Johns Hopkins University, Baltimore, MD 21218, USA
    Biophys J 96:4622-30. 2009
  6. doi request reprint Interactions of membrane active peptides with planar supported bilayers: an impedance spectroscopy study
    Janice Lin
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 28:6088-96. 2012
  7. doi request reprint Electrically addressable, biologically relevant surface-supported bilayers
    Janice Lin
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 26:12054-9. 2010
  8. doi request reprint The electrical response of bilayers to the bee venom toxin melittin: evidence for transient bilayer permeabilization
    Gregory Wiedman
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biochim Biophys Acta 1828:1357-64. 2013
  9. pmc Increased expression of the integral membrane protein ErbB2 in Chinese hamster ovary cells expressing the anti-apoptotic gene Bcl-xL
    Shannon O'Connor
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Protein Expr Purif 67:41-7. 2009
  10. pmc Assembly of the m2 tetramer is strongly modulated by lipid chain length
    Sandra Schick
    Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 99:1810-7. 2010

Collaborators

  • Min You
  • Xue Han
  • Yang Li
  • Yechiel Shai
  • William A Horton
  • Stephen H White
  • Ingo Köper
  • Mikhail Merzlyakov
  • Lijuan He
  • Edwin Li
  • Janice Lin
  • Lirong Chen
  • Peter C Searson
  • Fenghao Chen
  • William C Wimley
  • Jesse Placone
  • Sarvenaz Sarabipour
  • Vesselin Nikolov
  • Nadia Shobnam
  • Gregory Wiedman
  • Juan Cruz
  • Katherine Herman
  • Erika Ohsfeldt
  • Patrick J Stahl
  • Nuala Del Piccolo
  • Christopher Serrano
  • Peter Searson
  • Michael J Betenbaugh
  • Lawrence Novicky
  • John Szymanski
  • Sandra Schick
  • Ricky Soong
  • Shannon O'Connor
  • William K Chang
  • Greg Wiedman
  • Mihaela Mihailescu
  • Nitish Niphadkar
  • Sandra Carolina Agudelo
  • S Michael Yu
  • Linda Kristoffersen
  • Aram J Krauson
  • Thuy My T Le
  • Szu Han Huang
  • Deniz Baycin-Hizal
  • Juan C Cruz
  • Jennifer Motylinski
  • Catherine Degnin
  • Andrew R Hoffmann
  • Melanie Laederich
  • Tihomir Hristov
  • Tomer Cohen
  • Brian S Majors
  • Deniz Baycin
  • Aleksandar Radisic
  • Rachel Casas
  • Ivan Gitsov

Detail Information

Publications52

  1. pmc A look at arginine in membranes
    Kalina Hristova
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Membr Biol 239:49-56. 2011
    ..This is an active area of research, and there is no doubt that a consensus view of arginine in membranes will soon emerge...
  2. doi request reprint Measuring the energetics of membrane protein dimerization in mammalian membranes
    Lirong Chen
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Am Chem Soc 132:3628-35. 2010
    ..The results suggest that molecular crowding in cellular membranes has a dramatic effect on the strength of membrane protein interactions...
  3. ncbi request reprint Transmembrane helix heterodimerization in lipid bilayers: probing the energetics behind autosomal dominant growth disorders
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore MD 21218, USA
    J Mol Biol 358:1-7. 2006
    ....
  4. pmc Effect of the G375C and G346E achondroplasia mutations on FGFR3 activation
    Lijuan He
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 7:e34808. 2012
    ..We find that this mutation does not increase FGFR3 phosphorylation and decreases FGFR3 cross-linking propensity, a finding which raises questions whether this mutation is indeed a genetic cause for human dwarfism...
  5. pmc Energetics of ErbB1 transmembrane domain dimerization in lipid bilayers
    Lirong Chen
    Department of Materials Science and Engineering Johns Hopkins University, Baltimore, MD 21218, USA
    Biophys J 96:4622-30. 2009
    ....
  6. doi request reprint Interactions of membrane active peptides with planar supported bilayers: an impedance spectroscopy study
    Janice Lin
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 28:6088-96. 2012
    ....
  7. doi request reprint Electrically addressable, biologically relevant surface-supported bilayers
    Janice Lin
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 26:12054-9. 2010
    ....
  8. doi request reprint The electrical response of bilayers to the bee venom toxin melittin: evidence for transient bilayer permeabilization
    Gregory Wiedman
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biochim Biophys Acta 1828:1357-64. 2013
    ..The results directly demonstrate that the response of the bilayer to melittin at these concentrations cannot be explained by an equilibrium transmembrane pore model...
  9. pmc Increased expression of the integral membrane protein ErbB2 in Chinese hamster ovary cells expressing the anti-apoptotic gene Bcl-xL
    Shannon O'Connor
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Protein Expr Purif 67:41-7. 2009
    ..This approach may also be useful for other membrane proteins in which large quantities are needed for biophysical and structural studies...
  10. pmc Assembly of the m2 tetramer is strongly modulated by lipid chain length
    Sandra Schick
    Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 99:1810-7. 2010
    ..Thus, lipid chain length and bilayer thickness not only modulate peptide interactions, but could also be a major determinant of the association of transmembrane helices into functional membrane protein oligomers...
  11. ncbi request reprint The achondroplasia mutation does not alter the dimerization energetics of the fibroblast growth factor receptor 3 transmembrane domain
    Min You
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 45:5551-6. 2006
    ....
  12. pmc Physical basis behind achondroplasia, the most common form of human dwarfism
    Lijuan He
    Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 285:30103-14. 2010
    ..Instead, FGFR3 activity in achondroplasia is increased due to increased probability for phosphorylation of the unliganded mutant dimers. This finding has implications for the design of targeted molecular treatments for achondroplasia...
  13. pmc Protein folding in membranes: insights from neutron diffraction studies of a membrane beta-sheet oligomer
    Xue Han
    The Johns Hopkins University, Department of Materials Science and Engineering, Baltimore, Maryland 21218, USA
    Biophys J 94:492-505. 2008
    ....
  14. pmc The extracellular domain of fibroblast growth factor receptor 3 inhibits ligand-independent dimerization
    Lirong Chen
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Sci Signal 3:ra86. 2010
    ..Together, these results highlight the fine balance in the domain interactions that regulate RTK dimerization and signaling...
  15. ncbi request reprint Effect of pathogenic cysteine mutations on FGFR3 transmembrane domain dimerization in detergents and lipid bilayers
    Min You
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 46:11039-46. 2007
    ..These results and previous findings from cellular studies lead us to propose a conformational flexibility mechanism of receptor stabilization as a basis for disregulated FGFR3 signaling in thanatophoric dysplasia and achondroplasia...
  16. pmc The physical basis of FGFR3 response to fgf1 and fgf2
    Fenghao Chen
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States
    Biochemistry 50:8576-82. 2011
    ..As a result, fgf1 and fgf2 elicit a similar FGFR3c response at low, but not at high, concentrations. The results demonstrate the versatility of FGFR3c response to fgf1 and fgf2 and highlight the complexity in fgf signaling...
  17. pmc FGFR3 heterodimerization in achondroplasia, the most common form of human dwarfism
    Lijuan He
    Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 286:13272-81. 2011
    ..These results further our knowledge of FGFR3-associated bone disorders...
  18. ncbi request reprint Surface-supported bilayers with transmembrane proteins: role of the polymer cushion revisited
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 22:10145-51. 2006
    ..While proteins were always mobile if the bilayers were prepared using the directed assembly method, in the presence and absence of a PEG cushion, other bilayer assembly protocols resulted in complete lack of protein mobility...
  19. doi request reprint Effect of a polymer cushion on the electrical properties and stability of surface-supported lipid bilayers
    Janice Lin
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 26:3544-8. 2010
    ..These studies reveal the optimal PEG concentration that yields electrically robust bilayers and demonstrate the utility of the platform for future studies of membrane protein channels and membrane active peptides...
  20. ncbi request reprint FGFR3 dimer stabilization due to a single amino acid pathogenic mutation
    Edwin Li
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Mol Biol 356:600-12. 2006
    ..We show that this seemingly modest value can lead to a large increase in dimer fraction and thus profoundly affect RTK-mediated signal transduction...
  21. pmc Hill coefficient analysis of transmembrane helix dimerization
    Ricky Soong
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Membr Biol 230:49-55. 2009
    ....
  22. pmc Multiple consequences of a single amino acid pathogenic RTK mutation: the A391E mutation in FGFR3
    Fenghao Chen
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    PLoS ONE 8:e56521. 2013
    ..The results presented here provide new knowledge about the physical basis behind growth disorders and highlight the fact that a single RTK mutation may affect multiple steps in RTK activation...
  23. pmc Consequences of replacing EGFR juxtamembrane domain with an unstructured sequence
    Lijuan He
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Sci Rep 2:854. 2012
    ....
  24. pmc Production of plasma membrane vesicles with chloride salts and their utility as a cell membrane mimetic for biophysical characterization of membrane protein interactions
    Nuala Del Piccolo
    Department of Materials Science and Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, United States
    Anal Chem 84:8650-5. 2012
    ..We characterize the size distribution of the produced vesicles. We also show that these vesicles can be used for the biophysical characterization of interactions between membrane proteins...
  25. pmc Forster resonance energy transfer measurements of transmembrane helix dimerization energetics
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Methods Enzymol 450:107-27. 2008
    ..Such measurements can shed light on the molecular mechanism behind pathologies arising due to single-amino acid mutations in membrane proteins...
  26. pmc Studies of receptor tyrosine kinase transmembrane domain interactions: the EmEx-FRET method
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Membr Biol 215:93-103. 2007
    ....
  27. doi request reprint Pathogenic activation of receptor tyrosine kinases in mammalian membranes
    Lijuan He
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Mol Biol 384:1130-42. 2008
    ..They also suggest the cellular response to stimuli can be understood and predicted based on quantitative knowledge of interaction strengths between proteins involved in signaling...
  28. pmc Characterization of antimicrobial peptide activity by electrochemical impedance spectroscopy
    William K Chang
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biochim Biophys Acta 1778:2430-6. 2008
    ..The method presented not only provides additional information about the mode of action of a particular AMP, but offers a means of characterizing AMP activity in reproducible, well-defined quantitative terms...
  29. doi request reprint Utility of surface-supported bilayers in studies of transmembrane helix dimerization
    Edwin Li
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Struct Biol 168:53-60. 2009
    ....
  30. pmc Glycophorin A transmembrane domain dimerization in plasma membrane vesicles derived from CHO, HEK 293T, and A431 cells
    Sarvenaz Sarabipour
    Department of Materials Sciences and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biochim Biophys Acta 1828:1829-33. 2013
    ..Based on these results, we propose that any of the six plasma membrane preparations investigated here can be used as a model system for studies of membrane protein interactions...
  31. ncbi request reprint Spectral Förster resonance energy transfer detection of protein interactions in surface-supported bilayers
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Langmuir 22:6986-92. 2006
    ..However, the spectral properties of dyes can be altered in an unexpected manner in an ordered bilayer structure on a surface, such that fluorescence detection in surface-supported bilayers is not always trivial...
  32. pmc The A391E mutation enhances FGFR3 activation in the absence of ligand
    Fenghao Chen
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biochim Biophys Acta 1808:2045-50. 2011
    ..We find that the mutation enhances the ligand-independent activation propensity of the receptor by -1.7°kcal/mol. This value is consistent with the observed strength of hydrogen bonds in membranes, and supports the above hypothesis...
  33. ncbi request reprint Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon
    Vesselin Nikolov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 23:13040-5. 2007
    ..These results demonstrate that artificial bilayers formed by LB deposition have many unique advantages for electrical measurements of membranes and their components...
  34. pmc Neutron diffraction studies of fluid bilayers with transmembrane proteins: structural consequences of the achondroplasia mutation
    Xue Han
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 91:3736-47. 2006
    ..The presented results further our knowledge about basic amino-acid insertion into bilayers, and may lead to new insights into the mechanism of pathogenesis in achondroplasia...
  35. pmc Receptor tyrosine kinase transmembrane domains: Function, dimer structure and dimerization energetics
    Edwin Li
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA
    Cell Adh Migr 4:249-54. 2010
    ..Here we review these structures, and we discuss recent studies of RTK TM domain dimerization energetics, possible synergies between domains, and the effects of pathogenic RTK TM mutations on structure and dimerization...
  36. ncbi request reprint Role of receptor tyrosine kinase transmembrane domains in cell signaling and human pathologies
    Edwin Li
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 45:6241-51. 2006
    ..We review the current knowledge of pathology induction mechanisms due to these mutations, focusing on the structural and thermodynamic basis of pathogenic dimer stabilization...
  37. pmc A membrane-translocating peptide penetrates into bilayers without significant bilayer perturbations
    Juan Cruz
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA
    Biophys J 104:2419-28. 2013
    ..Perhaps most revealing, TP2 has the ability to penetrate deep into the bilayer without causing significant bilayer perturbations, a property that may help explain its ability to translocate without bilayer permeabilization...
  38. pmc Direct assessment of the effect of the Gly380Arg achondroplasia mutation on FGFR3 dimerization using quantitative imaging FRET
    Jesse Placone
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 7:e46678. 2012
    ..The data are consistent with the idea that the ACH mutation causes a structural change which affects both the stability and the activity of FGFR3 dimers in the absence of ligand...
  39. pmc High-throughput selection of transmembrane sequences that enhance receptor tyrosine kinase activation
    Lijuan He
    Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Mol Biol 412:43-54. 2011
    ..This work furthers our knowledge about the requirements for membrane protein interactions and the requirements for RTK activation in cells...
  40. ncbi request reprint Directed assembly of surface-supported bilayers with transmembrane helices
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 22:1247-53. 2006
    ..The described directed-assembly approach can be used to develop versatile bilayer platforms for studying membrane proteins interactions in native bilayer environments...
  41. doi request reprint Quantitative measurements of protein interactions in a crowded cellular environment
    Edwin Li
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Anal Chem 80:5976-85. 2008
    ..We also introduce the "quantitative imaging Foster resonance energy transfer" method as a tool that can yield protein interaction strengths within these vesicles...
  42. pmc Specific inhibition of a pathogenic receptor tyrosine kinase by its transmembrane domain
    Lijuan He
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biochim Biophys Acta 1808:253-9. 2011
    ..The results suggest that TM domain peptides could be exploited in the future for the development of specific inhibitors of mutant RTKs...
  43. pmc Viewing the bilayer hydrocarbon core using neutron diffraction
    Xue Han
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    J Membr Biol 227:123-31. 2009
    ..This method should have broad utility in structural studies of the bilayer response to protein insertion and folding in membranes...
  44. pmc Physical-chemical principles underlying RTK activation, and their implications for human disease
    Lijuan He
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biochim Biophys Acta 1818:995-1005. 2012
    ..We also review our understanding of pathogenic RTK mutations, and the underlying physical-chemical causes for the pathologies. This article is part of a Special Issue entitled: Protein Folding in Membranes...
  45. ncbi request reprint Bias-dependent admittance in hybrid bilayer membranes
    Vesselin Nikolov
    Department of Materials Science and Engineering, and Institute of NanoBioTechnology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Langmuir 22:7156-8. 2006
    ..This hybrid bilayer exhibits an electrical response analogous to a solid-state diode: the admittance is very low (<10(-)(7) Omega(-)(1) cm(-)(2)) over a wide potential range but increases exponentially at negative potentials...
  46. ncbi request reprint Impedance spectroscopy of bilayer membranes on single crystal silicon
    Janice Lin
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218
    Biointerphases 3:FA33. 2008
    ..The authors further report on the bias dependence of the impedance, and show that on highly doped n-type silicon, the membrane impedance can be measured over a 2 V range...
  47. doi request reprint Increased expression of the integral membrane proteins EGFR and FGFR3 in anti-apoptotic Chinese hamster ovary cell lines
    Erika Ohsfeldt
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA
    Biotechnol Appl Biochem 59:155-62. 2012
    ..This approach may be applied to improve stable expression of other membrane proteins in the future using mammalian cell lines with Bcl-x(L) or perhaps other anti-apoptotic genes...
  48. pmc Forster resonance energy transfer in liposomes: measurements of transmembrane helix dimerization in the native bilayer environment
    Min You
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Anal Biochem 340:154-64. 2005
    ..e., on the protein-to-lipid ratio), thereby yielding thermodynamic parameters that are directly relevant to processes in biological membranes...
  49. ncbi request reprint An experiment-based algorithm for predicting the partitioning of unfolded peptides into phosphatidylcholine bilayer interfaces
    Kalina Hristova
    Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 44:12614-9. 2005
    ..We present a simple algorithm for computing the absolute partitioning free energies of unfolded peptides into the phosphatidylcholine bilayer interface...
  50. ncbi request reprint Surface supported bilayer platform for studies of lateral association of proteins in membranes (Mini Review)
    Mikhail Merzlyakov
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biointerphases 3:FA80. 2008
    ..To satisfy these requirements, a new assembly method, termed "directed assembly" was developed. This assembly method could have broad utility in basic studies of proteins in membranes and in biotechnological applications...
  51. pmc FGFR3 transmembrane domain interactions persist in the presence of its extracellular domain
    Sarvenaz Sarabipour
    Department of Materials Sciences and Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 105:165-71. 2013
    ..The effect of the mutation on dimer stability in both cases is the same (∼-1.5 kcal/mol), suggesting that the interactions observed in simple TM-peptide model systems are relevant in a biological context...
  52. pmc On-the-resin N-terminal modification of long synthetic peptides
    Patrick J Stahl
    Department of Materials Science and Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Anal Biochem 424:137-9. 2012
    ..In all cases, the yields far exceeded those from commercially available preactivated compounds...