Research Topics
Genomes and Genes
| James G HermanSummaryAffiliation: Johns Hopkins University Country: USA Publications
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Publications
Defining a chromatin pattern that characterizes DNA-hypermethylated genes in colon cancer cellsKelly M McGarvey
Divisions of Cancer Biology, The Johns Hopkins Kimmel Cancer Center, Baltimore, Maryland 21231, USA
Cancer Res 68:5753-9. 2008..Our data have great relevance for the increasing interest in reexpression of DNA-hypermethylated genes for the treatment of cancer...
Methylation-specific polymerase chain reactionOliver Galm
Medizinische Klinik IV, Universitaetsklinikum Aachen, Aachen, Germany
Methods Mol Med 113:279-91. 2005..It is a rapid and cost-effective method that does not require radioactive reagents and can be used for the analysis of a large number of clinical samples...- Combined DNA methyltransferase and histone deacetylase inhibition in the treatment of myeloid neoplasmsSteven D Gore
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21287, USA
Cancer Res 66:6361-9. 2006..The promising percentage of major hematologic responses justifies the testing of such combinations in prospective randomized trials... - Hypermethylation pathways to colorectal cancer. Implications for prevention and detectionJames G Herman
Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins School of Medicine, Bunting Blaustein Cancer Research Building, 1650 Orleans, Room 543, Baltimore, MD 21231, USA
Gastroenterol Clin North Am 31:945-58. 2002..These changes compliment and lead to genetic changes that are well established as central to colorectal cancer progression. They may also prove useful in molecular detection approaches... - Gene silencing in cancer in association with promoter hypermethylationJames G Herman
Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins Medical Institutions, Baltimore, USA
N Engl J Med 349:2042-54. 2003 - Prognostic importance of promoter hypermethylation of multiple genes in esophageal adenocarcinomaMalcolm V Brock
Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
Clin Cancer Res 9:2912-9. 2003..The association of clinicopathological and biomolecular risk factors to survival and recurrence was performed using the Log-rank test and Cox proportional hazards model for multivariate analysis... - Circulating methylated DNAJames G Herman
Department of Oncology, Evelyn Grollman Glick Scholar, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
Ann N Y Acad Sci 1022:33-9. 2004..These factors are presented and their impact on the detection of methylated DNA circulating in the bloodstream is demonstrated... - Hypermethylation of the GATA genes in lung cancerMingzhou Guo
Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, MD 21287, USA
Clin Cancer Res 10:7917-24. 2004..In lung cancer, DNA hypermethylation is known to be a common event... - Aberrant silencing of cancer-related genes by CpG hypermethylation occurs independently of their spatial organization in the nucleusHariharan P Easwaran
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
Cancer Res 70:8015-24. 2010..These findings have important implications for understanding relationships between nuclear organization and gene expression patterns in cancer... - Epigenetic silencing of CDX2 is a feature of squamous esophageal cancerMingzhou Guo
Department of Oncology, Cancer Biology Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231 1000, USA
Int J Cancer 121:1219-26. 2007..Our results suggest that the inactivation of CDX2 in esophageal cancer associated with DNA methylation may be an important determinant of the squamous or non-adenomatous phenotype... - Promoter methylation of HIN-1 in the progression to esophageal squamous cancerMingzhou Guo
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
Epigenetics 3:336-41. 2008..These studies demonstrate HIN-1 silencing is associated with dense promoter region hypermethylation in esophageal cancer and suggest that methylation of HIN-1 is an early event in dysplastic transformation... - Accumulation of promoter methylation suggests epigenetic progression in squamous cell carcinoma of the esophagusMingzhou Guo
Cancer Biology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
Clin Cancer Res 12:4515-22. 2006..CONCLUSIONS: These findings suggest that epigenetic progression parallels the histologic changes observed in the progression of squamous carcinoma of the esophagus... - PcG proteins, DNA methylation, and gene repression by chromatin loopingVijay K Tiwari
Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University Medical Institutions, Baltimore, Maryland, USA
PLoS Biol 6:2911-27. 2008..The loops associate with a poised, low transcription state in EC cells and, with the addition of DNA methylation, completely repressed transcription in adult cancer cells... 
DNA methylation markers and early recurrence in stage I lung cancerMalcolm V Brock
Johns Hopkins Hospital, Baltimore, USA
N Engl J Med 358:1118-28. 2008..Despite optimal and early surgical treatment of non-small-cell lung cancer (NSCLC), many patients die of recurrent NSCLC. We investigated the association between gene methylation and recurrence of the tumor...- Epigenetic alteration of Wnt pathway antagonists in progressive glandular neoplasia of the lungJulien D F Licchesi
Cancer Biology Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
Carcinogenesis 29:895-904. 2008.... - Progressive chromatin repression and promoter methylation of CTNNA1 associated with advanced myeloid malignanciesYing Ye
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Cancer Res 69:8482-90. 2009.... - Epigenetic inactivation of the canonical Wnt antagonist SRY-box containing gene 17 in colorectal cancerWei Zhang
Division of Cancer Biology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
Cancer Res 68:2764-72. 2008.... - Hypermethylation of the GATA gene family in esophageal cancerMingzhou Guo
Cancer Biology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231 1000, USA
Int J Cancer 119:2078-83. 2006..Our studies demonstrate frequent silencing of GATA-4 and GATA-5, but not GATA-6, in human esophageal neoplasia associated with gene promoter hypermethylation... - DNA promoter hypermethylation of p16 and APC predicts neoplastic progression in Barrett's esophagusJean S Wang
Department of Medicine Gastroenterology, Johns Hopkins University, Baltimore, Maryland 21287, USA
Am J Gastroenterol 104:2153-60. 2009..We determined whether DNA promoter hypermethylation of genes frequently methylated in esophageal adenocarcinoma (p16 and APC) could be used as predictors of progression in BE... - Hypermethylation of ASC/TMS1 is a sputum marker for late-stage lung cancerEmi Ota Machida
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
Cancer Res 66:6210-8. 2006..Thus, hypermethylation of ASC/TMS1 is a marker for late-stage lung cancer and, in sputum, could predict prognosis in patients resected for early-stage disease... - Epigenetic regulation of protein phosphatase 2A (PP2A), lymphotactin (XCL1) and estrogen receptor alpha (ER) expression in human breast cancer cellsJudith C Keen
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
Cancer Biol Ther 3:1304-12. 2004..These gene products may be novel targets to be further explored in the development of new therapeutics for ER negative breast cancer... - hTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start siteRebekah L Zinn
Sidney Kimmel Comprehensive Cancer Center and Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
Cancer Res 67:194-201. 2007.... - Epigenetic differences in cytogenetically normal versus abnormal acute myeloid leukemiaElizabeth A Griffiths
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA
Epigenetics 5:590-600. 2010..Methylation of tumor suppression genes (TSGs) is common in myeloid malignancies. However, application of this as a molecular marker for risk stratification in patients with AML is limited... - An inactivating mutation in HDAC2 leads to dysregulation of apoptosis mediated by APAF1Christin L Hanigan
Sidney Kimmel Comprehensive Cancer Center and Graduate Program in Cellular and Molecular Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
Gastroenterology 135:1654-1664.e2. 2008..Histone deacetylases (HDACs) are important regulators of chromatin involved in silencing tumor suppressor genes. We examined mutation of HDAC2 and examined consequences of HDAC2 loss... - Methylation of TFPI2 in stool DNA: a potential novel biomarker for the detection of colorectal cancerSabine C Glöckner
Departments of Surgery, Oncology, and Pathology, Johns Hopkins University, Baltimore, Maryland 21287, USA
Cancer Res 69:4691-9. 2009..Detection of TFPI2 methylation in stool DNA may act as a useful adjunct to the noninvasive strategies for screening of CRCs in the future... - Acute myeloid leukemia is characterized by Wnt pathway inhibitor promoter hypermethylationElizabeth A Griffiths
Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
Leuk Lymphoma 51:1711-9. 2010.... - Alcohol consumption and the risk of nasopharyngeal carcinoma: a systematic reviewLiwei Chen
Center for Human Nutrition, Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland, USA
Nutr Cancer 61:1-15. 2009..Considered in total, the quantitative summaries of the case-control evidence suggest that heavy alcohol consumption is associated with an increased risk of NPC... - Can we improve the cytologic examination of malignant pleural effusions using molecular analysis?Malcolm V Brock
Division of Thoracic Surgery, Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
Ann Thorac Surg 80:1241-7. 2005..Deoxyribonucleic acid (DNA) methylation is a robust strategy for detecting cancer early in tissue. We hypothesized that DNA methylation would be more sensitive in diagnosing patients with malignant pleural effusions than cytology... - Promoter hypermethylation in sentinel lymph nodes as a marker for breast cancer recurrenceHetty E Carraway
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital, Baltimore, MD 21231, USA
Breast Cancer Res Treat 114:315-25. 2009..This study evaluates the methylation frequency and pattern in HNSN to determine if detection of hypermethylation of one or more genes is associated with an increased recurrence risk in node negative breast cancer... - Inhibition of SIRT1 reactivates silenced cancer genes without loss of promoter DNA hypermethylationKevin Pruitt
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, United States of America
PLoS Genet 2:e40. 2006.... - GATA-4 and GATA-5 transcription factor genes and potential downstream antitumor target genes are epigenetically silenced in colorectal and gastric cancerYoshimitsu Akiyama
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
Mol Cell Biol 23:8429-39. 2003..Selection for silencing of both upstream transcription factors and their target genes in GI cancers could indicate that epigenetic silencing of the involved genes provides a summated contribution to tumor progression... - Biomarkers for detection and prognosis of breast cancer identified by a functional hypermethylome screenJana Jeschke
Department of Surgery, Johns Hopkins University, Baltimore, MD, USA
Epigenetics 7:701-9. 2012..92) and validation cohort (HR = 2.87). Our study demonstrates an efficient method to utilize functional methylation changes in BC for the development of effective biomarkers for detection and prognosis prediction of BC... - Combination epigenetic therapy has efficacy in patients with refractory advanced non-small cell lung cancerRosalyn A Juergens
Department of Oncology, Johns Hopkins University, Baltimore, Maryland 21231, USA
Cancer Discov 1:598-607. 2011.... - Prognostic value of hMLH1 methylation and microsatellite instability in pancreatic endocrine neoplasmsMichael G House
Department of Surgery, The Johns Hopkins Medical Institutions, 600 N. Wolfe Street, Baltimore, MD 21287, USA
Surgery 134:902-8; discussion 909. 2003..017) at 5 years. CONCLUSION: As in hereditary nonpolyposis colorectal cancer in which MSI is associated with improved survival, methylation of hMLH1 leads to MSI in PENs and affords a favorable prognosis... - MS-qFRET: a quantum dot-based method for analysis of DNA methylationVasudev J Bailey
Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Genome Res 19:1455-61. 2009..The direct application of MS-qFRET on clinical samples offers great promise for its translational use in early cancer diagnosis, prognostic assessment of tumor behavior, as well as monitoring response to therapeutic agents... - CHFR promoter hypermethylation in colon cancer correlates with the microsatellite instability phenotypeJohann C Brandes
Cancer Biology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
Carcinogenesis 26:1152-6. 2005.... - Hypermethylation of a small CpGuanine-rich region correlates with loss of activator protein-2alpha expression during progression of breast cancerDonna B Douglas
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
Cancer Res 64:1611-20. 2004.... - DNA methylation analysis on a droplet-in-oil PCR arrayYi Zhang
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
Lab Chip 9:1059-64. 2009..These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner... - Dependence of histone modifications and gene expression on DNA hypermethylation in cancerJill A Fahrner
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, Maryland 21231-1000, USA
Cancer Res 62:7213-8. 2002..Our findings suggest a new paradigm-DNA methylation may directly, or indirectly by inhibiting transcription, maintain key repressive elements of the histone code at a hypermethylated gene promoter in cancer... - Epigenetic regulation of WNT signaling pathway genes in inflammatory bowel disease (IBD) associated neoplasiaMashaal Dhir
Department of Surgery, Johns Hopkins University, Baltimore, MD 21231, USA
J Gastrointest Surg 12:1745-53. 2008..This study examined the role of epigenetic silencing of WNT signaling pathway genes in the pathogenesis of IBD-associated neoplasia... - Evaluation of GATA-4 and GATA-5 methylation profiles in human pancreatic cancers indicate promoter methylation patterns distinct from other human tumor typesBaojin Fu
Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA
Cancer Biol Ther 6:1546-52. 2007..While hypermethylation of GATA-5 seems to be a universal feature among human tumors, infrequent methylation of GATA-4, and its corresponding overexpression, appears unique to pancreatic cancer from other tumor types reported thus far... - Inactivation of the tissue inhibitor of metalloproteinases-2 gene by promoter hypermethylation in lymphoid malignanciesOliver Galm
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, 1650 Orleans St, Baltimore, MD 21231, USA
Oncogene 24:4799-805. 2005..We conclude that promoter hypermethylation of TIMP-2 is a novel epigenetic event in the pathogenesis of lymphoid malignancies and may contribute to a more aggressive NHL phenotype... - Single-tube analysis of DNA methylation with silica superparamagnetic beadsVasudev J Bailey
Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, USA
Clin Chem 56:1022-5. 2010..This method includes many disconnected steps with associated losses of material, potentially reducing the analytical sensitivity required for analysis of challenging clinical samples... - Carotenoids and the risk of developing lung cancer: a systematic reviewLisa Gallicchio
The Prevention and Research Center, Mercy Medical Center, Baltimore, MD, USA
Am J Clin Nutr 88:372-83. 2008..Carotenoids are thought to have anti-cancer properties, but findings from population-based research have been inconsistent... - Promoter hypermethylation of resected bronchial margins: a field defect of changes?Mingzhou Guo
Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
Clin Cancer Res 10:5131-6. 2004..We hypothesized that DNA promoter hypermethylation changes at bronchial margins could be detected in patients with no histological evidence of malignancy and that they would reflect epigenetic events in the primary tumor... - Hypermethylation-associated Inactivation of the Cellular Retinol-Binding-Protein 1 Gene in Human CancerManel Esteller
The Johns Hopkins Oncology Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
Cancer Res 62:5902-5. 2002..Epigenetic disruption of CRBP1 is a common event in human cancer that may have important implications for cancer prevention and treatment using retinoids... - Tumor suppressor gene hypermethylation as a predictor of gastric stromal tumor behaviorMichael G House
Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, MD 21231 1000, USA
J Gastrointest Surg 7:1004-14; discussion 1014. 2003..This study supports the methylation status of E-cadherin as a prognostic marker for early GIST recurrence and survival... - Cancer as a manifestation of aberrant chromatin structureMalcolm V Brock
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21205 2196, USA
Cancer J 13:3-8. 2007..A more complete understanding of these key, regulatory events is vital in approaching a more rational drug therapy to various malignancies... - Histone modifications and silencing prior to DNA methylation of a tumor suppressor geneKurtis E Bachman
The Howard Hughes Medical Institute, The Sidney Kimmel Comprehensive Cancer Center, and Program in Cellular and Molecular Medicine, The Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, MD 21231, USA
Cancer Cell 3:89-95. 2003..These results have important implications for understanding the biochemical events underlying the silencing of tumor suppressor genes and the resultant growth suppression... - Surgical resection of limited disease small cell lung cancer in the new era of platinum chemotherapy: Its time has comeMalcolm V Brock
Division of Thoracic Surgery, Department of Surgery, The Johns Hopkinds Medical Institutions, Baltimore, MD, USA
J Thorac Cardiovasc Surg 129:64-72. 2005..Although resection is not the standard of care in treating small cell lung cancer, new platinum drugs and modern staging have allowed the role of surgery to be reevaluated... - Delayed diagnosis and elevated mortality in an urban population with HIV and lung cancer: implications for patient careMalcolm V Brock
Johns Hopkins Hospital, and Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21287, USA
J Acquir Immune Defic Syndr 43:47-55. 2006..Lung cancer is more common in HIV-infected patients than in the general population. We examined how effectively lung cancer was being diagnosed in our HIV-infected patients... - Methylation-specific PCRJulien D F Licchesi
Cancer Biology Program, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins, Baltimore, MD, USA
Methods Mol Biol 507:305-23. 2009.... - Genomic and epigenomic integration identifies a prognostic signature in colon cancerJoo Mi Yi
Department of Oncology, Johns Hopkins University, Baltimore, Maryland 20892 8110, USA
Clin Cancer Res 17:1535-45. 2011..The importance of genetic and epigenetic alterations maybe in their aggregate role in altering core pathways in tumorigenesis... - Epigenetic silencing of multiple genes in primary CNS lymphomaLinda C Chu
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
Int J Cancer 119:2487-91. 2006..Our study provides insight into the epigenetic alterations in PCNSL and provides potential biomarkers of disease... - Hypermethylation of the DNA repair gene O(6)-methylguanine DNA methyltransferase and survival of patients with diffuse large B-cell lymphomaManel Esteller
Division of Cancer Biology, The Johns Hopkins Oncology Center, Baltimore, MD 21231, USA
J Natl Cancer Inst 94:26-32. 2002..CONCLUSION: MGMT promoter hypermethylation appears to be a useful marker for predicting survival in patients with B-DLCL treated with multidrug regimens including cyclophosphamide... - A phase I dose-finding study of 5-azacytidine in combination with sodium phenylbutyrate in patients with refractory solid tumorsJianqing Lin
Chemical Therapeutics Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University, Baltimore, Maryland 21231, USA
Clin Cancer Res 15:6241-9. 2009..The pharmacokinetics, pharmacodynamics, and antineoplastic effects were also studied... - Heterozygous disruption of Hic1 predisposes mice to a gender-dependent spectrum of malignant tumorsWen Yong Chen
Division of Tumor Biology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Medical Institutions, 1650 Orleans Street, Baltimore, Maryland 21231, USA
Nat Genet 33:197-202. 2003..We conclude that HIC1 is a candidate tumor-suppressor gene for which loss of function in both mouse and human cancers is associated only with epigenetic modifications... - Comparing the DNA hypermethylome with gene mutations in human colorectal cancerKornel E Schuebel
Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
PLoS Genet 3:1709-23. 2007.... - Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancerHiromu Suzuki
Division of Tumor Biology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Medical Institutions, 1650 Orleans Street, Baltimore, Maryland 21231, USA
Nat Genet 36:417-22. 2004.... - Promoter hypermethylation of hallmark cancer genes in atypical adenomatous hyperplasia of the lungJulien D F Licchesi
Cancer Biology Program at the Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
Clin Cancer Res 14:2570-8. 2008.... - Promoter methylation profiles of tumor suppressor genes in intrahepatic and extrahepatic cholangiocarcinomaBin Yang
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD, USA
Mod Pathol 18:412-20. 2005..Taking advantage of the unique concurrent methylation profile of multiple genes in cholangiocarcinoma may facilitate the distinction of cholangiocarcinoma from benign biliary epithelium in clinical settings... - Progression of gene hypermethylation in gallstone disease leading to gallbladder cancerMichael G House
Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231 1000, USA
Ann Surg Oncol 10:882-9. 2003..Aberrant methylation of tumor-suppressor genes is associated with a loss of gene function that can afford selective growth advantages to sporadic neoplastic cells arising during gallbladder inflammation... - SOCS-1, a negative regulator of cytokine signaling, is frequently silenced by methylation in multiple myelomaOliver Galm
Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
Blood 101:2784-8. 2003..Silencing of the SOCS-1 gene may impair negative regulation of the Jak/STAT pathway and therefore result in greater responsiveness to cytokines, thus supporting survival and expansion of MM cells... - Short double-stranded RNA induces transcriptional gene silencing in human cancer cells in the absence of DNA methylationAngela H Ting
Graduate Program in Cellular and Molecular Medicine, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland 21231, USA
Nat Genet 37:906-10. 2005..Moreover, we could accomplish such silencing in a cancer cell line genetically modified to lack virtually any capacity to methylate DNA... - A genomic screen for genes upregulated by demethylation and histone deacetylase inhibition in human colorectal cancerHiromu Suzuki
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, Maryland 21231, USA
Nat Genet 31:141-9. 2002.... - Enzymatic regional methylation assay: a novel method to quantify regional CpG methylation densityOliver Galm
Oncology Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
Genome Res 12:153-7. 2002..We demonstrate a use of this technique in determining the methylation density of the promoter region of the tumor suppressor gene p15INK4B and changes that occur after treatment with demethylating agents... - Lung cancer in never smokers: molecular profiles and therapeutic implicationsCharles M Rudin
Johns Hopkins University School of Medicine, David H Koch Cancer Research Building, Room 544, 1550 Orleans Street, Baltimore, MD 21231, USA
Clin Cancer Res 15:5646-61. 2009..Focused analysis of molecular carcinogenesis in lung cancers in never smokers is needed, and may provide additional biologic insight with therapeutic implications for lung cancers in both ever smokers and never smokers... - Epigenetics in lung cancer: focus on progression and early lesionsJames G Herman
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University, Baltimore, MD 21231, USA
Chest 125:119S-22S. 2004 - Inactivation of LKB1/STK11 is a common event in adenocarcinomas of the lungMontserrat Sanchez-Cespedes
Department of Otolaryngology Head and Neck Surgery, Head and Neck Cancer Research Division, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2196, USA
Cancer Res 62:3659-62. 2002..Thus, our findings demonstrate for the first time that LKB1/STK11 inactivation is a very common event and may be integrally involved in the development of sporadic lung adenocarcinoma... - Molecular progression of promoter methylation in intraductal papillary mucinous neoplasms (IPMN) of the pancreasMichael G House
Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, MD, USA
Carcinogenesis 24:193-8. 2003..Detection of methylation within selected genes may afford an accurate diagnostic molecular marker and predictor of neoplastic behavior... - Early epigenetic changes and DNA damage do not predict clinical response in an overlapping schedule of 5-azacytidine and entinostat in patients with myeloid malignanciesTamer E Fandy
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, MD, USA
Blood 114:2764-73. 2009..This trial is registered with http://www.clinicaltrials.gov under NCT00101179... - Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumoursManel Esteller
Tumor Biology, The Johns Hopkins Oncology Center, Baltimore, MD 21231, USA
J Pathol 196:1-7. 2002..and apoptosis (DAPK, APAF-1, ...). The aberrant CpG island methylation can also be used as a biomarker of malignant cells and as a predictor of their behaviour, and may constitute a good target for future therapies... - A systematic profile of DNA methylation in human cancer cell linesMaria F Paz
Cancer Epigenetics Laboratory, Molecular Pathology Program, Spanish National Cancer Center, Carlos III Institute of Health, 28029 Madrid, Spain
Cancer Res 63:1114-21. 2003..Our data provide a basis for a better use of human cancer cell lines in basic and translational research with respect to their DNA methylation environment... - Germ-line variants in methyl-group metabolism genes and susceptibility to DNA methylation in normal tissues and human primary tumorsMaria F Paz
Cancer Epigenetics Laboratory, Molecular Pathology Program, Spanish National Cancer Research Center CNIO, 28029 Madrid, Spain
Cancer Res 62:4519-24. 2002..029). The existence of these associations may provide another example of the interplay between genetic and epigenetic factors in the cancer cell... - The fundamental role of epigenetics in hematopoietic malignanciesOliver Galm
Medizinische Klinik IV, Universitaetsklinikum Aachen, 52074 Aachen, Germany
Blood Rev 20:1-13. 2006..The ongoing exploration of basic events involved in altered gene transcription patterns and continued clinical investigative studies are helping to develop novel strategies for the diagnosis, prevention, and treatment of human cancer... - Effects of dietary folate and alcohol intake on promoter methylation in sporadic colorectal cancer: the Netherlands cohort study on diet and cancerManon van Engeland
The Research Institute GROW, Department of Pathology, University Maastricht, 6200 MD Maastricht, The Netherlands
Cancer Res 63:3133-7. 2003..085). Despite the size limitations of this study, these data suggest that folate and alcohol intake may be associated with changes in promoter hypermethylation in CRC... - Semiautomatic detection of DNA methylation at CpG islandsKyeong-Man Hong
National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Biotechniques 38:354, 356, 358. 2005 - K-ras mutations and RASSF1A promoter methylation in colorectal cancerManon van Engeland
Department of Pathology, University Maastricht, P O Box 616 6200 MD Maastricht, The Netherlands
Oncogene 21:3792-5. 2002..These data show that the majority of the studied CRCs with K-ras mutations lack RASSF1A promoter methylation, an event which occurs predominantly in K-ras wild-type CRCs (P=0.023, Chi-square test)... - Methylation-specific PCR unraveledSarah Derks
Research Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands
Cell Oncol 26:291-9. 2004..This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications... - Standardized approach for microsatellite instability detection in gastric carcinomasEva Musulen
Servei de Patologia, UDIAT CD, Corporacio Sanitaria Parc Tauli, Sabadell, Spain
Hum Pathol 35:335-42. 2004..We have developed a simple, sensitive, and specific approach to assess the presence of MSI in gastric cancer that may have clinical applications... - Hypermethylation in histologically distinct classes of breast cancerYoung Kyung Bae
Department of Pathology, Yeungnam University College of Medicine, Daegu, South Korea
Clin Cancer Res 10:5998-6005. 2004..Furthermore, the relatively unimodal distribution of methylation frequency for all three histologic types does not support there being a distinct CpG island methylator phenotype for breast cancer... - The clinical application of targeting cancer through histone acetylation and hypomethylationJill Gilbert
Division of Oncology and Hematology, Stanley S Scott Cancer Center, Louisiana State University, New Orleans, Louisiana 70112, USA
Clin Cancer Res 10:4589-96. 2004..Reversal of these epigenetic processes and up-regulation of genes important to prevent or reverse the malignant phenotype has therefore become a new therapeutic target in cancer treatment... - Methylated tumor-specific DNA as a plasma biomarker in patients with gliomaKyle D Weaver
Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Cancer Invest 24:35-40. 2006..Patients with systemic malignancies have substantial quantities of tumor-specific DNA in their plasma which may serve as a potential biomarker for tumor burden. This approach has not been studied in gliomas... - Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohortSteven A Belinsky
Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87108, USA
Cancer Res 66:3338-44. 2006.... - Epigenetic and genetic alterations of APC and CDH1 genes in lobular breast cancer: relationships with abnormal E-cadherin and catenin expression and microsatellite instabilityDavid Sarrio
Laboratory of Breast and Gynecological Cancer, Molecular Pathology Program, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain
Int J Cancer 106:208-15. 2003..The same tumors also show genetic and epigenetic alterations of APC gene. However, altered CDH1 and APC genes do not promote beta-catenin accumulation in this tumor type... - Convergence of mutation and epigenetic alterations identifies common genes in cancer that predict for poor prognosisTimothy A Chan
Cancer Biology Program, The Johns Hopkins Kimmel Cancer Center, Baltimore, Maryland, United States of America
PLoS Med 5:e114. 2008..We hypothesize that key tumor suppressor genes in cancer may be subject to mutation or hypermethylation... - Identification of epigenetically silenced genes in tumor endothelial cellsDebby M E I Hellebrekers
Department of Pathology, Research Institute for Growth and Development, Maastricht University and University Hospital, Maastricht, The Netherlands
Cancer Res 67:4138-48. 2007.... - A stem cell-like chromatin pattern may predispose tumor suppressor genes to DNA hypermethylation and heritable silencingJoyce E Ohm
Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center
Nat Genet 39:237-42. 2007.... - Aberrant hypermethylation of tumor suppressor genes in pancreatic endocrine neoplasmsMichael G House
Departments of Surgery
Ann Surg 238:423-31; discussion 431-2. 2003.... - Clinical implications of aberrant DNA methylation patterns in acute myelogenous leukemiaOliver Galm
Medizinische Klinik IV, Universitatsklinikum Aachen, Rheinisch Westfälische Technische Hochschule Aachen, Aachen, Germany
Ann Hematol 84:39-46. 2005..The growing knowledge of the role of epigenetics in the aberrant silencing of cancer-related genes provides a rationale and molecular basis for targeted therapeutic approaches with demethylating agents in AML... - DNA methylation mapping by tag-modified bisulfite genomic sequencingWeiguo Han
Laboratory of Human Toxicology and Molecular Epidemiology, Wadsworth Center, NYS Department of Health, Albany, NY 12201, USA
Anal Biochem 355:50-61. 2006..7-fold difference in GSTP1 mRNA expression (p<0.01). The tBGS method simplifies detailed methylation scanning of kilobase-scale genomic DNA, facilitating more ambitious genomic methylation mapping studies... - Inhibition of DNA methylation and histone deacetylation prevents murine lung cancerSteven A Belinsky
Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive SE, Albuquerque, New Mexico 87108, USA
Cancer Res 63:7089-93. 2003..Thus, reduction in DNMT and histone deacetylase activities that likely block epigenetically mediated gene silencing might provide a novel clinical strategy to help prevent the leading cause of cancer death in the United States... - Specific inhibition of DNMT1 by antisense oligonucleotides induces re-expression of estrogen receptor-alpha (ER) in ER-negative human breast cancer cell linesLan Yan
Sidney Kimmel Comprehensive Cancer at Johns Hopkins University Medical School, Baltimore, Maryland USA
Cancer Biol Ther 2:552-6. 2003..Our results suggest that specific inhibition of DNMT1 expression alone is sufficient to re-express ERa in human breast cancer cell lines... - Cancer epigenetics and methylationManel Esteller
Science 297:1807-8; discussion 1807-8. 2002 - Alterations of the p16/Rb/cyclin-D1 pathway in vulvar carcinoma, vulvar intraepithelial neoplasia, and lichen sclerosusEnrique Lerma
Department of Pathology, Hospital de la Santa Creu i Sant Pau, Autonomous University of Barcelona, Barcelona, Spain
Hum Pathol 33:1120-5. 2002....
Research Grants
- CHFR Methylation for Predicting Taxane Sensitivity in Lung and Esophageal CancerJames Herman; Fiscal Year: 2007..We will further develop a test for CHFR methylation and determine whether CHFR methylation can be used to predict which patients will respond to taxane based chemotherapies. ..