Thomas Hartung

Summary

Affiliation: Johns Hopkins Bloomberg School of Public Health
Country: USA

Publications

  1. ncbi request reprint Comparative analysis of the revised Directive 2010/63/EU for the protection of laboratory animals with its predecessor 86/609/EEC - a t4 report
    Thomas Hartung
    Johns Hopkins University, CAAT, USA
    ALTEX 27:285-303. 2010
  2. pmc Systematic review of membrane components of gram-positive bacteria responsible as pyrogens for inducing human monocyte/macrophage cytokine release
    Christoph Rockel
    Biochemical Pharmacology, University of Konstanz Konstanz, Germany
    Front Pharmacol 3:56. 2012
  3. pmc Look back in anger - what clinical studies tell us about preclinical work
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, MD 21205, USA
    ALTEX 30:275-91. 2013
  4. pmc Inflammatory findings on species extrapolations: humans are definitely no 70-kg mice
    Marcel Leist
    Doerenkamp Zbinden Chair for in vitro Toxicology and Biomedicine, University of Konstanz, Constance, Germany
    Arch Toxicol 87:563-7. 2013
  5. pmc Mechanistic validation
    Thomas Hartung
    Center for Alternatives to Animal Testing CAAT, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    ALTEX 30:119-30. 2013
  6. pmc Integrated testing strategies for safety assessments
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, MD 21205, USA
    ALTEX 30:3-18. 2013
  7. ncbi request reprint Alternative approaches for medical countermeasures to biological and chemical terrorism and warfare
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, MD 21205, USA
    ALTEX 29:251-60. 2012
  8. ncbi request reprint Systems toxicology
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, Center for Alternatives to Animal Testing CAAT, Baltimore, MD 21205, USA
    ALTEX 29:119-28. 2012
  9. doi request reprint From alternative methods to a new toxicology
    Thomas Hartung
    Dept Environmental Health Sciences, Johns Hopkins University, Baltimore, MD 21205, USA
    Eur J Pharm Biopharm 77:338-49. 2011
  10. doi request reprint Are in vitro tests suitable for regulatory use?
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, Department of Environmental Health Sciences, Center for Alternatives to Animal Testing, Doerenkamp Zbinden Chair for Evidence based Toxicology, Baltimore, Maryland 21205, USA
    Toxicol Sci 111:233-7. 2009

Collaborators

Detail Information

Publications62

  1. ncbi request reprint Comparative analysis of the revised Directive 2010/63/EU for the protection of laboratory animals with its predecessor 86/609/EEC - a t4 report
    Thomas Hartung
    Johns Hopkins University, CAAT, USA
    ALTEX 27:285-303. 2010
    ..It also represents a formal implementation of the 3Rs principle (Replacement, Reduction and Refinement of animal tests) put forward by Russel and Burch 1959...
  2. pmc Systematic review of membrane components of gram-positive bacteria responsible as pyrogens for inducing human monocyte/macrophage cytokine release
    Christoph Rockel
    Biochemical Pharmacology, University of Konstanz Konstanz, Germany
    Front Pharmacol 3:56. 2012
    ..However, this systematic review cannot exclude the possibility that other stimulatory compounds complement or substitute for LTA in being the counterpart to LPS in some Gram-positive bacteria...
  3. pmc Look back in anger - what clinical studies tell us about preclinical work
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, MD 21205, USA
    ALTEX 30:275-91. 2013
    ..Alarming studies from industry show miserable reproducibility of landmark studies. This paper discusses factors contributing to the lack of reproducibility and relevance of pre-clinical research...
  4. pmc Inflammatory findings on species extrapolations: humans are definitely no 70-kg mice
    Marcel Leist
    Doerenkamp Zbinden Chair for in vitro Toxicology and Biomedicine, University of Konstanz, Constance, Germany
    Arch Toxicol 87:563-7. 2013
    ..The low predictivity of animal experiments in research areas allowing direct comparisons of mouse versus human data puts strong doubt on the usefulness of animal data as key technology to predict human safety...
  5. pmc Mechanistic validation
    Thomas Hartung
    Center for Alternatives to Animal Testing CAAT, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    ALTEX 30:119-30. 2013
    ....
  6. pmc Integrated testing strategies for safety assessments
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, MD 21205, USA
    ALTEX 30:3-18. 2013
    ..This paper describes the state of the art of ITS and makes suggestions as to the definition, systematic combination, and quality assurance of ITS...
  7. ncbi request reprint Alternative approaches for medical countermeasures to biological and chemical terrorism and warfare
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, MD 21205, USA
    ALTEX 29:251-60. 2012
    ..The prospects of such approaches, their impact on the field of alternative approaches, and necessary complementary activities are discussed. The need to adapt quality assurance measures and experiences from validation is stressed...
  8. ncbi request reprint Systems toxicology
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, Center for Alternatives to Animal Testing CAAT, Baltimore, MD 21205, USA
    ALTEX 29:119-28. 2012
    ....
  9. doi request reprint From alternative methods to a new toxicology
    Thomas Hartung
    Dept Environmental Health Sciences, Johns Hopkins University, Baltimore, MD 21205, USA
    Eur J Pharm Biopharm 77:338-49. 2011
    ..Similarly, a portal for high-quality reviews of toxicological approaches and tools for the quantitative meta-analyses of data promise to serve as door opener for a new regulatory toxicology...
  10. doi request reprint Are in vitro tests suitable for regulatory use?
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, Department of Environmental Health Sciences, Center for Alternatives to Animal Testing, Doerenkamp Zbinden Chair for Evidence based Toxicology, Baltimore, Maryland 21205, USA
    Toxicol Sci 111:233-7. 2009
    ..Here, we would like following Hegel a summary of thesis and antithesis, but also try to outline the synthesis...
  11. ncbi request reprint Per aspirin ad astra
    Thomas Hartung
    CAAT, Johns Hopkins University, Baltimore, USA
    Altern Lab Anim 37:45-7. 2009
    ..The perspective lies in the Toxicology for the 21st Century initiatives, which aim to create a new approach from the scratch, by an evidence-based toxicology and a global "Human Toxicology Programme"...
  12. doi request reprint Lessons learned from alternative methods and their validation for a new toxicology in the 21st century
    Thomas Hartung
    Environmental Health Sciences, Center for Alternatives to Animal Testing, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Toxicol Environ Health B Crit Rev 13:277-90. 2010
    ..However, more importantly, if brought together they can result in a Human Toxicology Project and a real revolution in regulatory toxicology...
  13. ncbi request reprint First alternative method validated by a retrospective weight-of-evidence approach to replace the Draize eye test for the identification of non-irritant substances for a defined applicability domain
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, Center for Alternatives to Animal Testing CAAT, Baltimore, MD 21205, USA
    ALTEX 27:43-51. 2010
    ..Here, several of the people critically involved in this processes have summarized the important aspects of this history...
  14. pmc A toxicology for the 21st century--mapping the road ahead
    Thomas Hartung
    Center for Alternatives to Animal Testing, Department for Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21231, USA
    Toxicol Sci 109:18-23. 2009
    ..This will require intense conceptual steering to have all pieces of the puzzle come together...
  15. ncbi request reprint Evidence-based toxicology - the toolbox of validation for the 21st century?
    Thomas Hartung
    Johns Hopkins University, Dept Environmental Health Sciences, Center for Alternatives to Animal Testing, Baltimore, MD 21205, USA
    ALTEX 27:253-63. 2010
    ..e. the collection and evaluation of existing evidence from various sources, represents a step in this direction. Here, we will explore new toxicological approaches via evidence-based toxicology (EBT)...
  16. ncbi request reprint Food for Thought ... on mapping the human toxome
    Thomas Hartung
    CAAT, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD, USA
    ALTEX 28:83-93. 2011
    ..The concept is explained, and opportunities and obstacles are discussed, aiming to promote an initiative which will form the core of a Human Toxicology Project to implement Toxicology for the 21st Century...
  17. ncbi request reprint An expert consortium review of the EC-commissioned report "alternative (Non-Animal) methods for cosmetics testing: current status and future prospects - 2010"
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, Center for Alternatives to Animal Testing, Baltimore, MD 21205, USA
    ALTEX 28:183-209. 2011
    ..Additional opportunities lie in more global collaborations and the inclusion of other industry sectors...
  18. doi request reprint Cytokine induction by Gram-positive bacteria
    Christian Draing
    Biochemical Pharmacology, University of Konstanz, 78457 Konstanz, Germany
    Immunobiology 213:285-96. 2008
    ..The purification and characterization of the respective components of the bacterial cell wall has begun...
  19. pmc Heterozygous toll-like receptor 2 polymorphism does not affect lipoteichoic acid-induced chemokine and inflammatory responses
    Sonja von Aulock
    Biochemical Pharmacology, University of Konstanz, 78457 Konstanz, Germany
    Infect Immun 72:1828-31. 2004
    ..Blood from heterozygous polymorphic and wild-type donors reacted uniformly to LTA and Staphylococcus aureus. Thus, one functional allele for Toll-like receptor 2 suffices for full cytokine response...
  20. ncbi request reprint Polypropylene glycol is a selective binding inhibitor for LTA and other structurally related TLR2 agonists
    Christian Draing
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    Eur J Immunol 38:797-808. 2008
    ..In summary, PPG 1200 is a novel inhibitor of cytokine induction by TLR2 agonists, which interferes directly with the ligands...
  21. ncbi request reprint Development, validation and applications of the monocyte activation test for pyrogens based on human whole blood
    Stefanie Schindler
    Biochemical Pharmacology, University of Konstanz, Germany
    ALTEX 26:265-77. 2009
    ..This alternative method promises to replace the rabbit pyrogen test fully and to overcome several limitations of the LAL assay...
  22. doi request reprint High sensitivity pyrogen testing in water and dialysis solutions
    Mardas Daneshian
    Biochemical Pharmacology, University of Konstanz, Germany
    J Immunol Methods 336:64-70. 2008
    ..Spike recovery checks exclude interference. The test has been validated in an international study for pyrogen detection in injectable solutions...
  23. ncbi request reprint In vitro pyrogen test for toxic or immunomodulatory drugs
    Mardas Daneshian
    Biochemical Pharmacology, University of Konstanz, POB M668 78457 Konstanz, Germany
    J Immunol Methods 313:169-75. 2006
    ..This represents a promising new approach for the detection of pyrogenic contamination in drugs or in drugs containing interfering additives and should lead to improved safety levels...
  24. ncbi request reprint Heterozygous toll-like receptor 4 polymorphism does not influence lipopolysaccharide-induced cytokine release in human whole blood
    Sonja von Aulock
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    J Infect Dis 188:938-43. 2003
    ..These data demonstrate that the heterozygous Asp299Gly polymorphism does not exhibit a functional defect in cytokine release after the stimulation of blood monocytes...
  25. ncbi request reprint Endotoxin evaluation of eleven lipopolysaccharides by whole blood assay does not always correlate with Limulus amebocyte lysate assay
    Oliver Dehus
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    J Endotoxin Res 12:171-80. 2006
    ..coli. This results in an overestimation of pyrogenic burden by a factor of 500 in the sample when compared with the biological activity of highly purified P. aeruginosa LPS in human whole blood...
  26. ncbi request reprint International validation of pyrogen tests based on cryopreserved human primary blood cells
    Stefanie Schindler
    Biochemical Pharmacology, University of Konstanz, Universitätsstr 10, D 78457 Konstanz, Germany
    J Immunol Methods 316:42-51. 2006
    ..The test reached >90% sensitivity and specificity. In contrast to the LAL, the test was capable of detecting non-endotoxin pyrogens derived from Gram-positive bacteria or fungi...
  27. doi request reprint Presentation of lipoteichoic acid potentiates its inflammatory activity
    Susanne Deininger
    Biochemical Pharmacology, University of Konstanz, Universitaetsstr 10, 78464 Konstanz, Germany
    Immunobiology 213:519-29. 2008
    ..In practical terms, contamination of medical devices with components of Gram-positive bacteria may pose an underestimated inflammatory risk...
  28. pmc Use of synthetic derivatives to determine the minimal active structure of cytokine-inducing lipoteichoic acid
    Susanne Deininger
    Biochemical Pharmacology, University of Konstanz, POB M668, 78457 Konstanz, Germany
    Clin Vaccine Immunol 14:1629-33. 2007
    ..Lengthening of the chain with d-alanine-substituted backbone units results in increased cytokine-inducing potency and a more sensitive response...
  29. ncbi request reprint Granulocyte colony-stimulating factor (filgrastim) treatment primes for increased ex vivo inducible prostanoid release
    Sonja von Aulock
    Biochemical Pharmacology, University of Konstanz, Germany
    J Pharmacol Exp Ther 308:754-9. 2004
    ..These data show that G-CSF primes for increased PGE(2) and TxB(2) release. Cyclooxygenase inhibition counteracts neither the hematopoietic nor the anti-inflammatory activity of G-CSF but reduces side effects...
  30. pmc Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis
    Markus Mueller
    University of Konstanz, Biochemical Pharmacology, 78457 Konstanz, Germany
    J Clin Microbiol 44:3778-80. 2006
    ....
  31. doi request reprint Different Staphylococcus aureus whole bacteria mutated in putative pro-inflammatory membrane components have similar cytokine inducing activity
    Christoph Rockel
    Biochemical Pharmacology, University of Konstanz, Universitätsstr 10, 78464 Konstanz, Germany
    Immunobiology 216:316-21. 2011
    ....
  32. doi request reprint Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid
    Oliver Dehus
    Biochemical Pharmacology, University of Konstanz, Konstanz 78467, Germany
    Immunobiology 216:24-31. 2011
    ..68). The observed shift in LTA expression, probably accompanying the switch from the saprophytic to the virulent entity, indicates an important adaptation to the different structural requirements inside the host cells...
  33. ncbi request reprint Endogenous cortisol determines the circadian rhythm of lipopolysaccharide-- but not lipoteichoic acid--inducible cytokine release
    Corinna Hermann
    Biochemical Pharmacology, University of Konstanz, D 78457 Konstanz, Germany
    Eur J Immunol 36:371-9. 2006
    ..Furthermore, 10-50-fold higher HC concentrations as compared to LPS were required to block LTA-induced cytokine release. LTA, in contrast to LPS, failed to activate Jun kinase, a reported target for HC action...
  34. ncbi request reprint Cyclic AMP increases endogenous granulocyte colony-stimulating factor formation in monocytes and THP-1 macrophages despite attenuated TNF-alpha formation
    Lars Hareng
    Biochemical Pharmacology, Universitat Konstanz, Konstanz, Germany
    Eur J Immunol 33:2287-96. 2003
    ..This might have bearing for drug side effects, putative G-CSF mimetics and our understanding of G-CSF immunobiology...
  35. ncbi request reprint G-CSF: boosting endogenous production--a new strategy?
    Sonja von Aulock
    University of Konstanz, Biochemical Pharmacology, 78457 Konstanz, Germany
    Curr Opin Investig Drugs 5:1148-52. 2004
    ..New research into the production and regulation of G-CSF in health and disease may now enable tailored strategies to induce or boost G-CSF formation. Similarly, new forms of application may increase its effectiveness...
  36. ncbi request reprint Lipoteichoic acid from Staphylococcus aureus is a potent stimulus for neutrophil recruitment
    Sonja von Aulock
    Biochemical Pharmacology, University of Konstanz, 78457 Konstanz, Germany
    Immunobiology 208:413-22. 2003
    ..aureus of the same strain. LTA does not appear to activate neutrophils directly, but is a strong stimulus for the recruitment of phagocytes to the site of infection...
  37. ncbi request reprint Protecting more than animals
    Alan M Goldberg
    Johns Hopkins University, Center for Alternatives to Animal Testing, USA
    Sci Am 294:84-91. 2006
  38. ncbi request reprint Cryopreservation of human whole blood for pyrogenicity testing
    Stefanie Schindler
    Biochemical Pharmacology, University of Konstanz, D 78457 Konstanz, Germany
    J Immunol Methods 294:89-100. 2004
    ..Further possible applications include high-throughput screening for immunomodulators or toxins as well as preservation of patient samples for later analysis of cell functions...
  39. pmc Mapping the human toxome by systems toxicology
    Mounir Bouhifd
    Bloomberg School of Public Health, Johns Hopkins University, CAAT, Baltimore, MD, USA
    Basic Clin Pharmacol Toxicol 115:24-31. 2014
    ..The NIH Transformative Research Grant project Mapping the Human Toxome by Systems Toxicology aims at developing the tools for pathway mapping, annotation and validation as well as the respective knowledge base to share this information. ..
  40. ncbi request reprint Determination of the eicosanoid response to inflammatory stimuli in whole blood and its pharmacological modulation ex vivo
    Sonja von Aulock
    Biochemical Pharmacology, University of Konstanz, POB M655, 78457, Konstanz, Germany
    J Immunol Methods 277:53-63. 2003
    ..Furthermore, the assay could be used to characterize the immune status of patient groups or to monitor the course of disease...
  41. ncbi request reprint A new method to measure air-borne pyrogens based on human whole blood cytokine response
    Ilona Kindinger
    Biochemical Pharmacology, University of Konstanz, POB M655, 78457 Konstanz, Germany
    J Immunol Methods 298:143-53. 2005
    ..The method offers a test to measure the integral inflammatory capacity of air-borne microbial contaminations relevant to humans. It could thus be employed to assess air quality in different living and work environments...
  42. ncbi request reprint MDP and other muropeptides--direct and synergistic effects on the immune system
    Stephanie Traub
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    J Endotoxin Res 12:69-85. 2006
    ..This review discusses the structure and occurrence of muropeptides and gives a broad overview of their inflammatory and adjuvant activity and the possible involvement of receptors in these responses...
  43. ncbi request reprint Immunotoxicology: challenges in the 21st century and in vitro opportunities
    Thomas Hartung
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, USA and University of Konstanz, CAAT Europe, Germany
    ALTEX 30:411-26. 2013
    ....
  44. pmc Comparison of quantitative and semiquantitative enzyme-linked immunosorbent assays for immunoglobulin G against Chlamydophila pneumoniae to a microimmunofluorescence test for use with patients with respiratory tract infections
    Corinna Hermann
    Biochemical Pharmacology, University of Konstanz, 78457 Constance, Germany
    J Clin Microbiol 42:2476-9. 2004
    ..These results show that ELISAs that are fast and objective deliver seroprevalence results, sensitivities, and specificities that are very similar to those of the MIF test...
  45. ncbi request reprint Good Cell Culture Practice. ECVAM Good Cell Culture Practice Task Force Report 1
    Thomas Hartung
    Biochemical Pharmacology, University of Konstanz, 78457 Konstanz, Germany
    Altern Lab Anim 30:407-14. 2002
  46. ncbi request reprint In vitro pyrogen test--A new test method for solid medical devices
    Francesca Mazzotti
    Biochemical Pharmacology, University of Konstanz, Germany
    J Biomed Mater Res A 80:276-82. 2007
    ..This new application of the already internationally validated method promises to replace further rabbit pyrogen tests. It generates extremely sensitive results with an extended range of detectable pyrogenic contaminants...
  47. doi request reprint Assessment of pyrogenic contaminations with validated human whole-blood assay
    Mardas Daneshian
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    Nat Protoc 4:1709-21. 2009
    ..In comparison with the LAL, it also detects nonlipopolysaccharide pyrogens. In comparison with other monocyte activation tests it requires no cell preparation steps or cell culture facilities. The procedure takes 21-35 h to complete...
  48. pmc t⁴ workshop report. Nanotoxicology: "the end of the beginning" - signs on the roadmap to a strategy for assuring the safe application and use of nanomaterials
    Ellen K Silbergeld
    Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA
    ALTEX 28:236-41. 2011
    ..Finally, the group recommended that a reliable, open, curated database be developed that interfaces with existing databases to enable sharing of information...
  49. ncbi request reprint Green toxicology
    Alexandra Maertens
    Johns Hopkins University, Bloomberg School of Public Health, CAAT, Baltimore, MD, USA
    ALTEX 31:243-9. 2014
    ..Starting with the formation of a steering group and a series of workshops, the Green Toxicology concept is currently spreading internationally and is being refined via an iterative process. ..
  50. pmc Critical evaluation of urine-based PCR assay for diagnosis of Lyme borreliosis
    Carolin Rauter
    Biochemical Pharmacology, Faculty of Biology, University of Konstanz, Konstanz, Germany
    Clin Diagn Lab Immunol 12:910-7. 2005
    ..All 12 samples were negative by an alternative PCR targeting flagellin. The results of our study support doubts that urine is a suitable material for diagnosis of Lyme borreliosis...
  51. pmc Prevalence of Borrelia burgdorferi sensu lato genospecies in Ixodes ricinus ticks in Europe: a metaanalysis
    Carolin Rauter
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    Appl Environ Microbiol 71:7203-16. 2005
    ..afzelii and B. garinii are the most common Borrelia species, but the distribution of genospecies seems to vary in different regions in Europe. The most frequent coinfection by Borrelia species was found for B. garinii and B. valaisiana...
  52. pmc Comparison of the interleukin-1β-inducing potency of allergenic spores from higher fungi (basidiomycetes) in a cryopreserved human whole blood system
    Félix E Rivera-Mariani
    Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA
    Int Arch Allergy Immunol 163:154-62. 2014
    ..2013 S. Karger AG, Basel. ..
  53. ncbi request reprint Febrile temperatures attenuate IL-1 beta release by inhibiting proteolytic processing of the proform and influence Th1/Th2 balance by favoring Th2 cytokines
    Eva Maria Boneberg
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    J Immunol 171:664-8. 2003
    ..These results indicate that fever limits the production of the fever-inducing IL-1beta and also influences the adaptive immune response, favoring Th2 cytokine production...
  54. ncbi request reprint Re-evaluation of animal numbers and costs for in vivo tests to accomplish REACH legislation requirements for chemicals - a report by the transatlantic think tank for toxicology (t(4))
    Costanza Rovida
    CAAT Center for Alternatives to Animals Testing, Johns Hopkins University, Bloomberg School of Public Health, Doerenkamp Zbinden Chair for Evidence based Toxicology, Baltimore, MD 21205, USA
    ALTEX 26:187-208. 2009
    ..The overall result suggests a demand of 54 million vertebrate animals and testing costs of 9.5 billion euro. This clearly challenges the feasibility of the program without a major investment into high-throughput methodologies...
  55. ncbi request reprint Consensus report on the future of animal-free systemic toxicity testing
    Marcel Leist
    Center for Alternatives to Animal Testing, CAAT Europe, University of Konstanz, Konstanz, Germany
    ALTEX 31:341-56. 2014
    ..Finally, quantitative information on all factors and key events will be fed into systems biology models that allow a probabilistic risk assessment with flexible adaptation to exposure scenarios and individual risk factors. ..
  56. pmc Review: toxicometabolomics
    Mounir Bouhifd
    Johns Hopkins Bloomberg School of Public Health, Environmental Health Sciences, Chair for Evidence based Toxicology, Center for Alternatives to Animal Testing, 615 N Wolfe St, Baltimore, MD, 21205, USA
    J Appl Toxicol 33:1365-83. 2013
    ..In addition, this review lays out the prospects for further metabolomics application also in a regulatory context...
  57. pmc Borrelia burgdorferi-induced tolerance as a model of persistence via immunosuppression
    Isabel Diterich
    Biochemical Pharmacology, Faculty of Biology, University of Konstanz, Konstanz, Germany
    Infect Immun 71:3979-87. 2003
    ..In summary, we characterized tolerance induced by B. burgdorferi, describing a model of desensitization which might mirror the immunosuppression recently attributed to the persistence of Borrelia in immunocompetent hosts...
  58. pmc t4 workshop report: Pathways of Toxicity
    Andre Kleensang
    Johns Hopkins University, Bloomberg School of Public Health, Center for Alternatives to Animal Testing, Baltimore, MD, USA
    ALTEX 31:53-61. 2014
    ..Third, the workshop explored ways in which systems biology approaches could inform pathway annotation, and which resources are needed and available that can provide relevant PoT information to the diverse user communities. ..
  59. ncbi request reprint How to leverage an endogenous immune defense mechanism: the example of granulocyte colony-stimulating factor
    Thomas Hartung
    Biochemical Pharmacology, University of Konstanz, Konstanz, Germany
    Crit Care Med 31:S65-75. 2003
    ..In parallel, there was a clear tendency toward lowering the rate of postoperative septic complications under the administration of Filgrastim...
  60. ncbi request reprint Induction and regulation of endogenous granulocyte colony-stimulating factor formation
    Lars Hareng
    Biochemical Pharmacology, University of Konstanz, PO Box 655, D 78457 Konstanz, Germany
    Biol Chem 383:1501-17. 2002
    ..Lastly, the modulation of G-CSF formation by various pharmacological agents alerts to putative side effects of these drug treatments...
  61. ncbi request reprint A model of human whole blood lymphokine release for in vitro and ex vivo use
    Corinna Hermann
    Biochemical Pharmacology, University of Konstanz, 78457, Constance, Germany
    J Immunol Methods 275:69-79. 2003
    ..The model appears to be suitable for the evaluation of immunomodulatory agents in vitro as well as ex vivo...
  62. ncbi request reprint Evidence-based toxicology for the 21st century: opportunities and challenges
    Martin L Stephens
    Johns Hopkins University, Baltimore, MD, USA
    ALTEX 30:74-103. 2013
    ..Priority activities include conducting pilot studies to demonstrate the value of evidence-based approaches to toxicology, as well as conducting educational outreach on these approaches...