Gerald Warren Hart

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint O-GlcNAc cycling: how a single sugar post-translational modification is changing the way we think about signaling networks
    Chad Slawson
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biochem 97:71-83. 2006
  2. ncbi request reprint Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Nature 446:1017-22. 2007
  3. pmc Increased expression of beta-N-acetylglucosaminidase in erythrocytes from individuals with pre-diabetes and diabetes
    Kyoungsook Park
    Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA
    Diabetes 59:1845-50. 2010
  4. doi request reprint Nutrient regulation of immunity: O-GlcNAcylation regulates stimulus-specific NF-κB-dependent transcription
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Sci Signal 6:pe26. 2013
  5. pmc Chemical approaches to study O-GlcNAcylation
    Partha S Banerjee
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore 21205 2185, USA
    Chem Soc Rev 42:4345-57. 2013
  6. pmc How sugar tunes your clock
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA Electronic address
    Cell Metab 17:155-6. 2013
  7. pmc Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4
    Yoshihiro Akimoto
    Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Clin Proteomics 8:15. 2011
  8. pmc dbOGAP - an integrated bioinformatics resource for protein O-GlcNAcylation
    Jinlian Wang
    Department of Oncology, Georgetown University Medical Center, NW, Washington, DC 20007, USA
    BMC Bioinformatics 12:91. 2011
  9. pmc Glycomics hits the big time
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205 2185, USA
    Cell 143:672-6. 2010
  10. ncbi request reprint Hyperglycemia and the O-GlcNAc transferase in rat aortic smooth muscle cells: elevated expression and altered patterns of O-GlcNAcylation
    Y Akimoto
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham Station, Alabama 35294, USA
    Arch Biochem Biophys 389:166-75. 2001

Research Grants

  1. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2009
  2. GLYCOSYLATION AND CANCER
    Gerald Warren Hart; Fiscal Year: 2010
  3. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2009
  4. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2003
  5. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2003
  6. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2007
  7. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2007
  8. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2002
  9. GLYCOCONJUGATES IN DEVELOPMENT AND IMMUNITY
    Gerald Hart; Fiscal Year: 2007
  10. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2004

Collaborators

  • Natasha E Zachara
  • J B Lowe
  • Jamey D Marth
  • Spencer Knapp
  • Wei Dong Gao
  • David R M Graham
  • Linda C Hsieh-Wilson
  • Yoshihiro Akimoto
  • Cheng Xin Gong
  • Eduardo Marban
  • Fei Liu
  • K Iqbal
  • Zihao Wang
  • Lance Wells
  • Stephen A Whelan
  • Chad Slawson
  • Wagner B Dias
  • Kyoungsook Park
  • Michael P Housley
  • Keith Vosseller
  • Jeffrey Shabanowitz
  • Donald F Hunt
  • Namrata D Udeshi
  • Chutikarn Butkinaree
  • Win D Cheung
  • Kaoru Sakabe
  • M Daniel Lane
  • Kazuo Kamemura
  • Partha S Banerjee
  • Jinlian Wang
  • Quira Zeidan
  • Ping Hu
  • Christopher D Saudek
  • Yuan Gao
  • Pere Puigserver
  • Joseph T Rodgers
  • Garland L Crawford
  • Ronald J Copeland
  • Steven P Jones
  • Genaro A Ramirez-Correa
  • Kaya Bork
  • Howard J Goldberg
  • Frank I Comer
  • Sai Prasad N Iyer
  • Jin Won Cho
  • Manabu Torii
  • Zhang Zhi Hu
  • Hongfang Liu
  • Antonio De Maio
  • Shino Shimoji
  • Philip D Compton
  • Meaghan O'Malley
  • Lakshmanan Thiruneelakantapillai
  • Frank Comer
  • Werner Reutter
  • Anne M Murphy
  • Aruni Bhatnagar
  • Wenhai Jin
  • Yasushi Teshima
  • Christoph Kannicht
  • Sabine Nöhring
  • Wenke Weidemann
  • Bradford G Hill
  • Timothy J Kelly
  • Sidney W Whiteheart
  • Xin Zhong
  • Marjan Gucek
  • Megan Barber
  • T Lakshmanan
  • Sungjin Park
  • Gladys A Ngoh
  • Cecilia Vecoli
  • John W Bullen
  • Rüdiger Horstkorte
  • Akhilesh Pandey
  • Catharine I Whiteside
  • I George Fantus
  • Lisa K Kreppel
  • Brian E Wadzinski
  • Jun ichi Miyazaki
  • Janet M Cronshaw
  • Michael J Matunis
  • Robert N Cole
  • Chen Chen
  • Bradley K Hayes
  • James A Mahoney
  • Antony Rosen

Detail Information

Publications64

  1. ncbi request reprint O-GlcNAc cycling: how a single sugar post-translational modification is changing the way we think about signaling networks
    Chad Slawson
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biochem 97:71-83. 2006
    ..This review will focus on recent work involving O-GlcNAc in sensing the environment and regulating signaling cascades...
  2. ncbi request reprint Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Nature 446:1017-22. 2007
    ..Glycosylation with O-GlcNAc modulates signalling, and influences protein expression, degradation and trafficking. Emerging data indicate that O-GlcNAc glycosylation has a role in the aetiology of diabetes and neurodegeneration...
  3. pmc Increased expression of beta-N-acetylglucosaminidase in erythrocytes from individuals with pre-diabetes and diabetes
    Kyoungsook Park
    Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA
    Diabetes 59:1845-50. 2010
    ..We investigated the expression of these two enzymes in erythrocytes of human subjects with diabetes or pre-diabetes...
  4. doi request reprint Nutrient regulation of immunity: O-GlcNAcylation regulates stimulus-specific NF-κB-dependent transcription
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Sci Signal 6:pe26. 2013
    ..This study not only illustrates how specific stimuli that act on the same transcription factor can elicit the expression of particular sets of genes, it also suggests a possible mechanism for autoimmunity in diabetes. ..
  5. pmc Chemical approaches to study O-GlcNAcylation
    Partha S Banerjee
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore 21205 2185, USA
    Chem Soc Rev 42:4345-57. 2013
    ..Availability of facile glycomic techniques are allowing for the exponential growth in the study of protein O-GlcNAcylation and are helping to elucidate key biological roles of this novel PTM...
  6. pmc How sugar tunes your clock
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA Electronic address
    Cell Metab 17:155-6. 2013
    ..Two papers in this issue of Cell Metabolism reveal that O-GlcNAcylation of clock proteins, which is dependent on nutrients, adjusts our circadian clock (Kaasik et al., 2013; Li et al., 2013)...
  7. pmc Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4
    Yoshihiro Akimoto
    Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Clin Proteomics 8:15. 2011
    ..abstract:..
  8. pmc dbOGAP - an integrated bioinformatics resource for protein O-GlcNAcylation
    Jinlian Wang
    Department of Oncology, Georgetown University Medical Center, NW, Washington, DC 20007, USA
    BMC Bioinformatics 12:91. 2011
    ..Furthermore, a bioinformatics resource for O-GlcNAcylation is lacking, and an O-GlcNAcylation site prediction tool is much needed...
  9. pmc Glycomics hits the big time
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205 2185, USA
    Cell 143:672-6. 2010
    ..Recent advances in glycomics reveal the scope and scale of their functional roles and their impact on human disease...
  10. ncbi request reprint Hyperglycemia and the O-GlcNAc transferase in rat aortic smooth muscle cells: elevated expression and altered patterns of O-GlcNAcylation
    Y Akimoto
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham Station, Alabama 35294, USA
    Arch Biochem Biophys 389:166-75. 2001
    ..These results suggest that the abnormal O-GlcNAc modification of intracellular proteins may be involved in glucose toxicity to vascular tissues...
  11. ncbi request reprint Glycobiology and cancer: meeting summary and future diections
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    Cancer Biol Ther 3:233-7. 2004
  12. ncbi request reprint Nucleocytoplasmic glycosylation, O-GlcNAc: identification and site mapping
    Natasha Elizabeth Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 284:175-94. 2004
    ....
  13. pmc Two-dimensional gel-based approaches for the assessment of N-Linked and O-GlcNAc glycosylation in human and simian immunodeficiency viruses
    David R M Graham
    Department of Medicine, Division of Cardiology, The JHU Bayview Proteomics Center, The Johns Hopkins University, Baltimore, MD 21224, USA
    Proteomics 8:4919-30. 2008
    ..This approach will permit correlation of virus glycosylation status with pathological severity and may serve as a rapid screen of viruses from physiological samples for further study by more advanced MS methodology...
  14. pmc Elevated nucleocytoplasmic glycosylation by O-GlcNAc results in insulin resistance associated with defects in Akt activation in 3T3-L1 adipocytes
    Keith Vosseller
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 99:5313-8. 2002
    ..These results suggest that elevation of O-GlcNAc levels attenuate insulin signaling and contribute to the mechanism by which increased flux through the HSP leads to insulin resistance in adipocytes...
  15. pmc The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA damage/repair and other cellular pathways
    Natasha E Zachara
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Amino Acids 40:793-808. 2011
    ..Supporting this hypothesis, we have shown that DNA-PK is O-GlcNAc modified in response to numerous forms of cellular stress...
  16. ncbi request reprint Perturbations in O-linked beta-N-acetylglucosamine protein modification cause severe defects in mitotic progression and cytokinesis
    Chad Slawson
    Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 280:32944-56. 2005
    ..These data suggest that dynamic O-GlcNAc processing is a pivotal regulatory component of the cell cycle, controlling cell cycle progression by regulating mitotic phosphorylation, cyclin expression, and cell division...
  17. doi request reprint Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University Medical School, Baltimore, Maryland, USA
    Curr Protoc Mol Biol . 2002
    ..This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group...
  18. pmc Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis
    Zihao Wang
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Sci Signal 3:ra2. 2010
    ....
  19. ncbi request reprint O-GlcNAc a sensor of cellular state: the role of nucleocytoplasmic glycosylation in modulating cellular function in response to nutrition and stress
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Biochim Biophys Acta 1673:13-28. 2004
    ..Recently, O-GlcNAc has been implicated in the etiology of type II diabetes, the regulation of stress response pathways, and in the regulation of the proteasome...
  20. pmc Enrichment and site mapping of O-linked N-acetylglucosamine by a combination of chemical/enzymatic tagging, photochemical cleavage, and electron transfer dissociation mass spectrometry
    Zihao Wang
    Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell Proteomics 9:153-60. 2010
    ..Using this strategy, eight O-GlcNAc sites were mapped from a tau-enriched sample from rat brain. Sites of GlcNAcylation were characterized on important neuronal proteins such as tau, synucleins, and methyl CpG-binding protein 2...
  21. ncbi request reprint Diverse regulation of protein function by O-GlcNAc: a nuclear and cytoplasmic carbohydrate post-translational modification
    Keith Vosseller
    Johns Hopkins University School of Medicine, Department of Biological Chemistry, Baltimore, MD 21218, USA
    Curr Opin Chem Biol 6:851-7. 2002
    ....
  22. ncbi request reprint Proteomic approaches to analyze the dynamic relationships between nucleocytoplasmic protein glycosylation and phosphorylation
    Stephen A Whelan
    Johns Hopkins University School of Medicine, Department of Biological Chemistry, 725 N Wolfe St, Baltimore, MD, USA
    Circ Res 93:1047-58. 2003
    ..We discuss how recent technological innovations will expand our present understanding of O-GlcNAc and what the implications are for diabetes and cardiovascular complications...
  23. ncbi request reprint Dynamic interplay between O-glycosylation and O-phosphorylation of nucleocytoplasmic proteins: alternative glycosylation/phosphorylation of THR-58, a known mutational hot spot of c-Myc in lymphomas, is regulated by mitogens
    Kazuo Kamemura
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 277:19229-35. 2002
    ..These data suggest that hierarchical phosphorylation of Ser-62 and Thr-58 and alternative glycosylation/phosphorylation of Thr-58 together regulate the myriad functions of c-Myc in cells...
  24. ncbi request reprint Dynamic nuclear and cytoplasmic glycosylation: enzymes of O-GlcNAc cycling
    Sai Prasad N Iyer
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Biochemistry 42:2493-9. 2003
  25. ncbi request reprint Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications
    Lance Wells
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA
    Mol Cell Proteomics 1:791-804. 2002
    ..In addition, our studies emphasize the importance of distinguishing between O-phosphate versus O-GlcNAc when mapping sites of serine and threonine post-translational modification using beta-elimination/Michael addition methods...
  26. ncbi request reprint Dynamic interplay between O-linked N-acetylglucosaminylation and glycogen synthase kinase-3-dependent phosphorylation
    Zihao Wang
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    Mol Cell Proteomics 6:1365-79. 2007
    ..Taken together, these data indicated the complex interplay between phosphorylation and O-GlcNAcylation that occurs within signaling networks...
  27. ncbi request reprint O-GlcNAc modification in diabetes and Alzheimer's disease
    Wagner B Dias
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185
    Mol Biosyst 3:766-72. 2007
    ..Alzheimer's disease and type II diabetes represent two metabolic disorders where dysfunctional protein GlcNAcylation/phosphorylation may be important for disease pathology...
  28. pmc O-GlcNAc cycling enzymes associate with the translational machinery and modify core ribosomal proteins
    Quira Zeidan
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    Mol Biol Cell 21:1922-36. 2010
    ..Our results not only establish that O-GlcNAcylation extensively modifies RPs, but also suggest that O-GlcNAc play important roles in regulating translation and ribosome biogenesis...
  29. pmc Cross-talk between GlcNAcylation and phosphorylation: site-specific phosphorylation dynamics in response to globally elevated O-GlcNAc
    Zihao Wang
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    Proc Natl Acad Sci U S A 105:13793-8. 2008
    ....
  30. pmc Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone code
    Kaoru Sakabe
    Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:19915-20. 2010
    ..Finally, we show that histone O-GlcNAcylation changes during mitosis and with heat shock. Taken together, these data show that O-GlcNAc cycles dynamically on histones and can be considered part of the histone code...
  31. ncbi request reprint Identification of O-GlcNAc sites on proteins
    Stephen A Whelan
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Enzymol 415:113-33. 2006
    ..These protocols include galactosyltransferase labeling, immunoblotting, using mass spectrometry based on beta-elimination followed by Michael addition with dithiothreitol, and chemoenzymatic labeling, enrichment, and detection...
  32. ncbi request reprint O-GlcNAc turns twenty: functional implications for post-translational modification of nuclear and cytosolic proteins with a sugar
    Lance Wells
    Johns Hopkins School of Medicine, Department of Biological Chemistry, 725 N Wolfe St, Baltimore, MD 21205, USA
    FEBS Lett 546:154-8. 2003
    ..Finally, we will discuss future directions and the working model of O-GlcNAc serving as a nutrient sensor...
  33. ncbi request reprint Fine-tuning ER-beta structure with PTMs
    Gerald W Hart
    Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Chem Biol 13:923-4. 2006
    ..NMR, CD, and molecular dynamics analyses of model peptides show that these alternative modifications induce different peptide conformations, providing a molecular basis for their differential regulation of protein function...
  34. ncbi request reprint Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase
    Lance Wells
    Department of Biological Chemistry and Medicine, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 277:1755-61. 2002
    ..These studies support the identification of this protein as an O-GlcNAcase and identify important interactions and modifications that may regulate the enzyme and O-GlcNAc cycling...
  35. ncbi request reprint The emerging significance of O-GlcNAc in cellular regulation
    Natasha E Zachara
    The Department of Biological Chemistry, The Johns Hopkins University Medical School, Baltimore, Maryland 21205 2185, USA
    Chem Rev 102:431-8. 2002
  36. ncbi request reprint Dynamic interplay between O-GlcNAc and O-phosphate: the sweet side of protein regulation
    Chad Slawson
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    Curr Opin Struct Biol 13:631-6. 2003
    ..For example, the increased levels of O-GlcNAc that occur in diabetes are associated with decreased insulin responsiveness in adipocytes...
  37. ncbi request reprint Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cells
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 279:30133-42. 2004
    ..O-GlcNAc regulates both the rates and extent of the stress-induced induction of heat shock proteins, providing a molecular basis for these findings...
  38. ncbi request reprint Dynamic interplay between O-glycosylation and O-phosphorylation of nucleocytoplasmic proteins: a new paradigm for metabolic control of signal transduction and transcription
    Kazuo Kamemura
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Prog Nucleic Acid Res Mol Biol 73:107-36. 2003
    ..Various aspects of subcellular localization, association with binding partners, activity, and/or turnover of these proteins appear to be regulated by dynamic glycosylation/ phosphorylation in response to cellular signals or stages...
  39. pmc O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress
    Chutikarn Butkinaree
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Biochim Biophys Acta 1800:96-106. 2010
    ..Their specificities are controlled by many transiently associated targeting subunits. As methods for detecting O-GlcNAc have improved our understanding of O-GlcNAc's functions has grown rapidly...
  40. pmc A PGC-1alpha-O-GlcNAc transferase complex regulates FoxO transcription factor activity in response to glucose
    Michael P Housley
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:5148-57. 2009
    ....
  41. pmc Regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification
    Wagner B Dias
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:21327-37. 2009
    ..This is the first example of an O-GlcNAc/phosphate cycle involving O-GlcNAc transferase/kinase cross-talk...
  42. pmc Regulation of insulin receptor substrate 1 (IRS-1)/AKT kinase-mediated insulin signaling by O-Linked beta-N-acetylglucosamine in 3T3-L1 adipocytes
    Stephen A Whelan
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 285:5204-11. 2010
    ..We conclude that one of the steps at which O-GlcNAc contributes to insulin resistance is by inhibiting phosphorylation at the Y(608)XXM PI3K p85 binding motif in IRS-1 and possibly at PDK1 as well...
  43. ncbi request reprint O-GlcNAc modification: a nutritional sensor that modulates proteasome function
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University Medical School, Baltimore, MD 21205 2185, USA
    Trends Cell Biol 14:218-21. 2004
    ..Moreover, increased glycosylation of the 19S (or PA700) regulatory subcomplex has been correlated with decreased proteasomal activity, suggesting a new model of proteasomal regulation...
  44. ncbi request reprint O-GlcNAc: a regulatory post-translational modification
    Lance Wells
    Department of Biological Chemistry, Johns Hopkins School of Medicine, 517 WBSB, 725 N Wolfe St, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 302:435-41. 2003
    ..In this review, we will focus on the enzymes that add/remove O-GlcNAc, the functional impact of O-GlcNAc modification, and the current working model for O-GlcNAc as a nutrient sensor...
  45. ncbi request reprint Site-specific interplay between O-GlcNAcylation and phosphorylation in cellular regulation
    Ping Hu
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205 2185, USA
    FEBS Lett 584:2526-38. 2010
    ..The extensive crosstalk between O-GlcNAcylation and phosphorylation represents a new paradigm for cellular signaling...
  46. ncbi request reprint The transcription factor PDX-1 is post-translationally modified by O-linked N-acetylglucosamine and this modification is correlated with its DNA binding activity and insulin secretion in min6 beta-cells
    Yuan Gao
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
    Arch Biochem Biophys 415:155-63. 2003
    ..These data suggest that O-GlcNAcylation may be involved in the regulation of PDX-1 DNA binding activity and in glucose-stimulated insulin secretion in beta-cells...
  47. ncbi request reprint Structural and functional diversity of glycoconjugates: a formidable challenge to the glycoanalyst
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 213:3-24. 2003
  48. ncbi request reprint O-GlcNAc transferase is in a functional complex with protein phosphatase 1 catalytic subunits
    Lance Wells
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:38466-70. 2004
    ....
  49. pmc O-GlcNAc regulates FoxO activation in response to glucose
    Michael P Housley
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 283:16283-92. 2008
    ..GlcNAcylation of FoxO provides a new mechanism for direct nutrient control of transcription to regulate metabolism and stress response through control of FoxO1 activity...
  50. pmc Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resistance and glucose toxicity
    Ronald J Copeland
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
    Am J Physiol Endocrinol Metab 295:E17-28. 2008
    ....
  51. pmc Regulation of the O-linked beta-N-acetylglucosamine transferase by insulin signaling
    Stephen A Whelan
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    J Biol Chem 283:21411-7. 2008
    ..We conclude that insulin stimulates the tyrosine phosphorylation and activity of OGT...
  52. pmc Characterization of beta-N-acetylglucosaminidase cleavage by caspase-3 during apoptosis
    Chutikarn Butkinaree
    Departments of Biological Chemistry and Neuroscience, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    J Biol Chem 283:23557-66. 2008
    ..These studies support the identification of O-GlcNAcase as a caspase-3 substrate with a novel caspase-3 cleavage site and provide insight about O-GlcNAcase regulation during apoptosis...
  53. pmc A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin
    Chad Slawson
    Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 19:4130-40. 2008
    ..Together, these data demonstrate that the O-GlcNAc cycling enzymes associate with kinases and phosphatases at M phase to regulate the posttranslational status of vimentin...
  54. pmc Site-specific GlcNAcylation of human erythrocyte proteins: potential biomarker(s) for diabetes
    Zihao Wang
    Department of Biological Chemistry, School of Medicine, The Johns Hopkins University, Baltimore, Maryland, USA
    Diabetes 58:309-17. 2009
    ..Here, we investigated the extent of GlcNAcylation on human erythrocyte proteins and compared site-specific GlcNAcylation on erythrocyte proteins from diabetic and normal individuals...
  55. pmc O-linked GlcNAc modification of cardiac myofilament proteins: a novel regulator of myocardial contractile function
    Genaro A Ramirez-Correa
    Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Circ Res 103:1354-8. 2008
    ..This study provides the first identification of O-GlcNAcylation sites in cardiac myofilament proteins and demonstrates their potential role in regulating myocardial contractile function...
  56. ncbi request reprint O-GlcNAc modification of nucleocytoplasmic proteins and diabetes
    Yoshihiro Akimoto
    Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, 181 8611, Japan
    Med Mol Morphol 38:84-91. 2005
    ..In this article, we review the current data regarding the relationship between O-GlcNAc modification and diabetes...
  57. ncbi request reprint Cell signaling, the essential role of O-GlcNAc!
    Natasha E Zachara
    Department of Biological Chemistry, Johns Hopkins Singapore, 31 Biopolis Way, 02 01 The Nanos, 138669 Singapore
    Biochim Biophys Acta 1761:599-617. 2006
    ..Recent research has highlighted key roles for O-GlcNAc serving as a nutrient sensor in regulating insulin signaling, the cell cycle, and calcium handling, as well as the cellular stress response...
  58. ncbi request reprint N-propanoylmannosamine interferes with O-GlcNAc modification of the tyrosine 3-monooxygenase and stimulates dopamine secretion
    Kaya Bork
    Charite Universitatsmedizin Berlin, Berlin, Germany
    J Neurosci Res 86:647-52. 2008
    ..We therefore propose a model in which the application of ManNProp leads to increased phosphorylation and activation of tyrosine 3-monooxygenase, which in turn leads to an increased synthesis of dopamine...
  59. ncbi request reprint Localization of the O-GlcNAc transferase and O-GlcNAc-modified proteins in rat cerebellar cortex
    Yoshihiro Akimoto
    Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181 8611, Tokyo, Japan
    Brain Res 966:194-205. 2003
    ..Thus, by modulating the phosphorylation or protein associations of key regulatory and cytoskeletal proteins, O-GlcNAc is likely important to many functions of the cerebellum...
  60. ncbi request reprint Posttranslational, reversible O-glycosylation is stimulated by high glucose and mediates plasminogen activator inhibitor-1 gene expression and Sp1 transcriptional activity in glomerular mesangial cells
    Howard J Goldberg
    Department of Medicine, Mount Sinai Hospital and University Health Network, Toronto, Ontario, Canada M5G 1X5
    Endocrinology 147:222-31. 2006
    ..These findings demonstrate for the first time that among the pathways served by the HBP, O-GlcNAcylation, is obligatory for HG-induced PAI-1 gene expression and Sp1 transcriptional activation in mesangial cells...
  61. ncbi request reprint Cardioprotection by N-acetylglucosamine linkage to cellular proteins
    Steven P Jones
    Institute of Molecular Cardiology, University of Louisville School of Medicine, Louisville, KY 40202, USA
    Circulation 117:1172-82. 2008
    ..Considering this, we hypothesized that augmentation of O-GlcNAc levels represents an endogenously recruitable mechanism of cardioprotection...
  62. pmc Murine platelets are not regulated by O-linked beta-N-acetylglucosamine
    Garland L Crawford
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, 741 S Limestone, BBSRB, Lexington, KY 40536, USA
    Arch Biochem Biophys 474:220-4. 2008
    ..These data suggest that while the modification occurs in platelets, their activity is not globally sensitive to O-GlcNAc levels...
  63. pmc O-GlcNAcylation regulates phosphorylation of tau: a mechanism involved in Alzheimer's disease
    Fei Liu
    Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA
    Proc Natl Acad Sci U S A 101:10804-9. 2004
    ....
  64. ncbi request reprint Elevation of the post-translational modification of proteins by O-linked N-acetylglucosamine leads to deterioration of the glucose-stimulated insulin secretion in the pancreas of diabetic Goto-Kakizaki rats
    Yoshihiro Akimoto
    Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Glycobiology 17:127-40. 2007
    ..These results indicate that elevation of the O-GlcNAcylation of proteins leads to deterioration of insulin secretion in the pancreas of diabetic GK rats, further providing evidence for the role of O-GlcNAc in the insulin secretion...

Research Grants32

  1. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2009
    ..These studies will result in novel avenues for therapeutics. ..
  2. GLYCOSYLATION AND CANCER
    Gerald Warren Hart; Fiscal Year: 2010
    ..This study will reveal the interplay between this sugar and phosphate in the regulation of signaling cascades important to cancer, and will open new avenues for drug development. ..
  3. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2009
    ..This study will reveal the interplay between this sugar and phosphate in the regulation of signaling cascades important to cancer, and will open new avenues for drug development. ..
  4. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2003
    ....
  5. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2003
    ..Furthermore, the saccharide appears to modulate the transcriptional activities and cellular associations of key transcription factors involved in transformation to the oncogenic phenotype. ..
  6. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2007
    ..These studies will result in novel avenues for therapeutics. ..
  7. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2007
    ..These studies will result in novel avenues for therapeutics. ..
  8. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2002
    ....
  9. GLYCOCONJUGATES IN DEVELOPMENT AND IMMUNITY
    Gerald Hart; Fiscal Year: 2007
    ..These studies are fundamentally important to the regulation of transcription and signal transduction processes important to virtually all aspects of development and disease. ..
  10. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2004
    ..abstract_text> ..
  11. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2001
    ..Furthermore, the saccharide appears to modulate the transcriptional activities and cellular associations of key transcription factors involved in transformation to the oncogenic phenotype. ..
  12. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2000
    ..Furthermore, the saccharide appears to modulate the transcriptional activities and cellular associations of key transcription factors involved in transformation to the oncogenic phenotype. ..
  13. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 1999
    ..Furthermore, the saccharide appears to modulate the transcriptional activities and cellular associations of key transcription factors involved in transformation to the oncogenic phenotype. ..
  14. ROLE OF CELL-SURFACE GLYCOSYLATION IN TUMOR METASTASIS
    Gerald Hart; Fiscal Year: 1991
    ..These studies are using the best available animal models of tumor metastasis to examine the mechanisms behind and the roles of altered glycosylation in generation of the metastatic phenotype...
  15. ROLE OF CELL-SURFACE GLYCOSYLATION IN TUMOR METASTASIS
    Gerald Hart; Fiscal Year: 1990
    ..These studies are using the best available animal models of tumor metastasis to examine the mechanisms behind and the roles of altered glycosylation in generation of the metastatic phenotype...
  16. ROLE OF CELL-SURFACE GLYCOSYLATION IN TUMOR METASTASIS
    Gerald Hart; Fiscal Year: 1992
    ..These studies are using the best available animal models of tumor metastasis to examine the mechanisms behind and the roles of altered glycosylation in generation of the metastatic phenotype...
  17. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2004
    ....
  18. O-GlcNAc:Developing New Tool for Assessment of Glycemia
    Gerald Hart; Fiscal Year: 2007
    ..The project also represents a new inter-disciplinary, inter-departmental basic science-clinical research collaboration between Biological Chemistry and The Department of Medicine. ..
  19. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Hart; Fiscal Year: 2006
    ..These studies will result in novel avenues for therapeutics. ..
  20. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2002
    ..Furthermore, the saccharide appears to modulate the transcriptional activities and cellular associations of key transcription factors involved in transformation to the oncogenic phenotype. ..
  21. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Warren Hart; Fiscal Year: 2010
    ..Data from these experiments will reveal totally new avenues for developing treatments for diabetes. ..
  22. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2007
    ..abstract_text> ..
  23. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2006
    ..abstract_text> ..
  24. GLYCOSYLATION AND CANCER
    Gerald Hart; Fiscal Year: 2005
    ..abstract_text> ..