Research Topics
| Gerald Warren HartSummaryAffiliation: Johns Hopkins University Country: USA Publications
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Detail Information
Publications
O-GlcNAc cycling: how a single sugar post-translational modification is changing the way we think about signaling networksChad Slawson
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
J Cell Biochem 97:71-83. 2006..This review will focus on recent work involving O-GlcNAc in sensing the environment and regulating signaling cascades...
Chemical approaches to study O-GlcNAcylationPartha S Banerjee
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore 21205 2185, USA
Chem Soc Rev 42:4345-57. 2013..Availability of facile glycomic techniques are allowing for the exponential growth in the study of protein O-GlcNAcylation and are helping to elucidate key biological roles of this novel PTM...- How sugar tunes your clockGerald W Hart
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
Cell Metab 17:155-6. 2013..Two papers in this issue of Cell Metabolism reveal that O-GlcNAcylation of clock proteins, which is dependent on nutrients, adjusts our circadian clock (Kaasik et al., 2013; Li et al., 2013)... 
Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4Yoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
Clin Proteomics 8:15. 2011..abstract:..- dbOGAP - an integrated bioinformatics resource for protein O-GlcNAcylationJinlian Wang
Department of Oncology, Georgetown University Medical Center, NW, Washington, DC 20007, USA
BMC Bioinformatics 12:91. 2011..Furthermore, a bioinformatics resource for O-GlcNAcylation is lacking, and an O-GlcNAcylation site prediction tool is much needed... - Hyperglycemia and the O-GlcNAc transferase in rat aortic smooth muscle cells: elevated expression and altered patterns of O-GlcNAcylationY Akimoto
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham Station, Alabama 35294, USA
Arch Biochem Biophys 389:166-75. 2001..These results suggest that the abnormal O-GlcNAc modification of intracellular proteins may be involved in glucose toxicity to vascular tissues... - Glycobiology and cancer: meeting summary and future diectionsGerald W Hart
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2185, USA
Cancer Biol Ther 3:233-7. 2004 - Glycomics hits the big timeGerald W Hart
Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205 2185, USA
Cell 143:672-6. 2010..Recent advances in glycomics reveal the scope and scale of their functional roles and their impact on human disease... - Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteinsGerald W Hart
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
Nature 446:1017-22. 2007..Glycosylation with O-GlcNAc modulates signalling, and influences protein expression, degradation and trafficking. Emerging data indicate that O-GlcNAc glycosylation has a role in the aetiology of diabetes and neurodegeneration... - Nucleocytoplasmic glycosylation, O-GlcNAc: identification and site mappingNatasha Elizabeth Zachara
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Methods Mol Biol 284:175-94. 2004.... - Two-dimensional gel-based approaches for the assessment of N-Linked and O-GlcNAc glycosylation in human and simian immunodeficiency virusesDavid R M Graham
Department of Medicine, Division of Cardiology, The JHU Bayview Proteomics Center, The Johns Hopkins University, Baltimore, MD 21224, USA
Proteomics 8:4919-30. 2008..This approach will permit correlation of virus glycosylation status with pathological severity and may serve as a rapid screen of viruses from physiological samples for further study by more advanced MS methodology... - The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA damage/repair and other cellular pathwaysNatasha E Zachara
The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
Amino Acids 40:793-808. 2011..Supporting this hypothesis, we have shown that DNA-PK is O-GlcNAc modified in response to numerous forms of cellular stress... - Elevated nucleocytoplasmic glycosylation by O-GlcNAc results in insulin resistance associated with defects in Akt activation in 3T3-L1 adipocytesKeith Vosseller
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 99:5313-8. 2002..These results suggest that elevation of O-GlcNAc levels attenuate insulin signaling and contribute to the mechanism by which increased flux through the HSP leads to insulin resistance in adipocytes... - Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesisZihao Wang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Sci Signal 3:ra2. 2010.... - Perturbations in O-linked beta-N-acetylglucosamine protein modification cause severe defects in mitotic progression and cytokinesisChad Slawson
Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2185, USA
J Biol Chem 280:32944-56. 2005..These data suggest that dynamic O-GlcNAc processing is a pivotal regulatory component of the cell cycle, controlling cell cycle progression by regulating mitotic phosphorylation, cyclin expression, and cell division... - Detection and analysis of proteins modified by O-linked N-acetylglucosamineNatasha E Zachara
The Johns Hopkins University Medical School, Baltimore, Maryland, USA
Curr Protoc Mol Biol . 2002..This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group... - Dynamic nuclear and cytoplasmic glycosylation: enzymes of O-GlcNAc cyclingSai Prasad N Iyer
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205-2185, USA
Biochemistry 42:2493-9. 2003 - O-GlcNAc a sensor of cellular state: the role of nucleocytoplasmic glycosylation in modulating cellular function in response to nutrition and stressNatasha E Zachara
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205-2185, USA
Biochim Biophys Acta 1673:13-28. 2004..Recently, O-GlcNAc has been implicated in the etiology of type II diabetes, the regulation of stress response pathways, and in the regulation of the proteasome... - Enrichment and site mapping of O-linked N-acetylglucosamine by a combination of chemical/enzymatic tagging, photochemical cleavage, and electron transfer dissociation mass spectrometryZihao Wang
Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Mol Cell Proteomics 9:153-60. 2010..Using this strategy, eight O-GlcNAc sites were mapped from a tau-enriched sample from rat brain. Sites of GlcNAcylation were characterized on important neuronal proteins such as tau, synucleins, and methyl CpG-binding protein 2... - Diverse regulation of protein function by O-GlcNAc: a nuclear and cytoplasmic carbohydrate post-translational modificationKeith Vosseller
Johns Hopkins University School of Medicine, Department of Biological Chemistry, Baltimore, MD 21218, USA
Curr Opin Chem Biol 6:851-7. 2002.... - Proteomic approaches to analyze the dynamic relationships between nucleocytoplasmic protein glycosylation and phosphorylationStephen A Whelan
Johns Hopkins University School of Medicine, Department of Biological Chemistry, 725 N Wolfe St, Baltimore, MD, USA
Circ Res 93:1047-58. 2003..We discuss how recent technological innovations will expand our present understanding of O-GlcNAc and what the implications are for diabetes and cardiovascular complications... - Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modificationsLance Wells
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA
Mol Cell Proteomics 1:791-804. 2002..In addition, our studies emphasize the importance of distinguishing between O-phosphate versus O-GlcNAc when mapping sites of serine and threonine post-translational modification using beta-elimination/Michael addition methods... - Dynamic interplay between O-glycosylation and O-phosphorylation of nucleocytoplasmic proteins: alternative glycosylation/phosphorylation of THR-58, a known mutational hot spot of c-Myc in lymphomas, is regulated by mitogensKazuo Kamemura
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2185, USA
J Biol Chem 277:19229-35. 2002..These data suggest that hierarchical phosphorylation of Ser-62 and Thr-58 and alternative glycosylation/phosphorylation of Thr-58 together regulate the myriad functions of c-Myc in cells... - Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cellsNatasha E Zachara
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2185, USA
J Biol Chem 279:30133-42. 2004..O-GlcNAc regulates both the rates and extent of the stress-induced induction of heat shock proteins, providing a molecular basis for these findings... - Dynamic interplay between O-glycosylation and O-phosphorylation of nucleocytoplasmic proteins: a new paradigm for metabolic control of signal transduction and transcriptionKazuo Kamemura
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Prog Nucleic Acid Res Mol Biol 73:107-36. 2003..Various aspects of subcellular localization, association with binding partners, activity, and/or turnover of these proteins appear to be regulated by dynamic glycosylation/ phosphorylation in response to cellular signals or stages... - Dynamic interplay between O-linked N-acetylglucosaminylation and glycogen synthase kinase-3-dependent phosphorylationZihao Wang
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
Mol Cell Proteomics 6:1365-79. 2007..Taken together, these data indicated the complex interplay between phosphorylation and O-GlcNAcylation that occurs within signaling networks... - O-GlcNAc modification in diabetes and Alzheimer's diseaseWagner B Dias
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185
Mol Biosyst 3:766-72. 2007..Alzheimer's disease and type II diabetes represent two metabolic disorders where dysfunctional protein GlcNAcylation/phosphorylation may be important for disease pathology... - O-GlcNAc cycling enzymes associate with the translational machinery and modify core ribosomal proteinsQuira Zeidan
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
Mol Biol Cell 21:1922-36. 2010..Our results not only establish that O-GlcNAcylation extensively modifies RPs, but also suggest that O-GlcNAc play important roles in regulating translation and ribosome biogenesis... - Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone codeKaoru Sakabe
Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 107:19915-20. 2010..Finally, we show that histone O-GlcNAcylation changes during mitosis and with heat shock. Taken together, these data show that O-GlcNAc cycles dynamically on histones and can be considered part of the histone code... - O-GlcNAc turns twenty: functional implications for post-translational modification of nuclear and cytosolic proteins with a sugarLance Wells
Johns Hopkins School of Medicine, Department of Biological Chemistry, 725 N. Wolfe St, Baltimore, MD 21205, USA
FEBS Lett 546:154-8. 2003..Finally, we will discuss future directions and the working model of O-GlcNAc serving as a nutrient sensor... - Identification of O-GlcNAc sites on proteinsStephen A Whelan
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Methods Enzymol 415:113-33. 2006..These protocols include galactosyltransferase labeling, immunoblotting, using mass spectrometry based on beta-elimination followed by Michael addition with dithiothreitol, and chemoenzymatic labeling, enrichment, and detection... - Cross-talk between GlcNAcylation and phosphorylation: site-specific phosphorylation dynamics in response to globally elevated O-GlcNAcZihao Wang
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
Proc Natl Acad Sci U S A 105:13793-8. 2008.... - Dynamic interplay between O-GlcNAc and O-phosphate: the sweet side of protein regulationChad Slawson
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2185, USA
Curr Opin Struct Biol 13:631-6. 2003..For example, the increased levels of O-GlcNAc that occur in diabetes are associated with decreased insulin responsiveness in adipocytes... - Fine-tuning ER-beta structure with PTMsGerald W Hart
Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
Chem Biol 13:923-4. 2006..NMR, CD, and molecular dynamics analyses of model peptides show that these alternative modifications induce different peptide conformations, providing a molecular basis for their differential regulation of protein function... - The emerging significance of O-GlcNAc in cellular regulationNatasha E Zachara
The Department of Biological Chemistry, The Johns Hopkins University Medical School, Baltimore, Maryland 21205-2185, USA
Chem Rev 102:431-8. 2002 - Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcaseLance Wells
Department of Biological Chemistry and Medicine, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 277:1755-61. 2002..These studies support the identification of this protein as an O-GlcNAcase and identify important interactions and modifications that may regulate the enzyme and O-GlcNAc cycling... - O-linked GlcNAc modification of cardiac myofilament proteins: a novel regulator of myocardial contractile functionGenaro A Ramirez-Correa
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Circ Res 103:1354-8. 2008..This study provides the first identification of O-GlcNAcylation sites in cardiac myofilament proteins and demonstrates their potential role in regulating myocardial contractile function... - Regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modificationWagner B Dias
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 284:21327-37. 2009..This is the first example of an O-GlcNAc/phosphate cycle involving O-GlcNAc transferase/kinase cross-talk... - A PGC-1alpha-O-GlcNAc transferase complex regulates FoxO transcription factor activity in response to glucoseMichael P Housley
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 284:5148-57. 2009.... - Structural and functional diversity of glycoconjugates: a formidable challenge to the glycoanalystGerald W Hart
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Methods Mol Biol 213:3-24. 2003 - O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stressChutikarn Butkinaree
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Biochim Biophys Acta 1800:96-106. 2010..Their specificities are controlled by many transiently associated targeting subunits. As methods for detecting O-GlcNAc have improved our understanding of O-GlcNAc's functions has grown rapidly... - Regulation of insulin receptor substrate 1 (IRS-1)/AKT kinase-mediated insulin signaling by O-Linked beta-N-acetylglucosamine in 3T3-L1 adipocytesStephen A Whelan
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
J Biol Chem 285:5204-11. 2010..We conclude that one of the steps at which O-GlcNAc contributes to insulin resistance is by inhibiting phosphorylation at the Y(608)XXM PI3K p85 binding motif in IRS-1 and possibly at PDK1 as well... - Increased expression of beta-N-acetylglucosaminidase in erythrocytes from individuals with pre-diabetes and diabetesKyoungsook Park
Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA
Diabetes 59:1845-50. 2010..We investigated the expression of these two enzymes in erythrocytes of human subjects with diabetes or pre-diabetes... - O-GlcNAc: a regulatory post-translational modificationLance Wells
Department of Biological Chemistry, Johns Hopkins School of Medicine, 517 WBSB, 725 N. Wolfe St, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 302:435-41. 2003..In this review, we will focus on the enzymes that add/remove O-GlcNAc, the functional impact of O-GlcNAc modification, and the current working model for O-GlcNAc as a nutrient sensor... - Site-specific interplay between O-GlcNAcylation and phosphorylation in cellular regulationPing Hu
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205 2185, USA
FEBS Lett 584:2526-38. 2010..The extensive crosstalk between O-GlcNAcylation and phosphorylation represents a new paradigm for cellular signaling... - Site-specific GlcNAcylation of human erythrocyte proteins: potential biomarker(s) for diabetesZihao Wang
Department of Biological Chemistry, School of Medicine, The Johns Hopkins University, Baltimore, Maryland, USA
Diabetes 58:309-17. 2009..Here, we investigated the extent of GlcNAcylation on human erythrocyte proteins and compared site-specific GlcNAcylation on erythrocyte proteins from diabetic and normal individuals... - A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentinChad Slawson
Department of Biological Chemistry, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
Mol Biol Cell 19:4130-40. 2008..Together, these data demonstrate that the O-GlcNAc cycling enzymes associate with kinases and phosphatases at M phase to regulate the posttranslational status of vimentin... - O-GlcNAc transferase is in a functional complex with protein phosphatase 1 catalytic subunitsLance Wells
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 279:38466-70. 2004.... - O-GlcNAc modification: a nutritional sensor that modulates proteasome functionNatasha E Zachara
Department of Biological Chemistry, The Johns Hopkins University Medical School, Baltimore, MD 21205-2185, USA
Trends Cell Biol 14:218-21. 2004..Moreover, increased glycosylation of the 19S (or PA700) regulatory subcomplex has been correlated with decreased proteasomal activity, suggesting a new model of proteasomal regulation... - The transcription factor PDX-1 is post-translationally modified by O-linked N-acetylglucosamine and this modification is correlated with its DNA binding activity and insulin secretion in min6 beta-cellsYuan Gao
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205-2185, USA
Arch Biochem Biophys 415:155-63. 2003..These data suggest that O-GlcNAcylation may be involved in the regulation of PDX-1 DNA binding activity and in glucose-stimulated insulin secretion in beta-cells... - Characterization of beta-N-acetylglucosaminidase cleavage by caspase-3 during apoptosisChutikarn Butkinaree
Departments of Biological Chemistry and Neuroscience, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
J Biol Chem 283:23557-66. 2008..These studies support the identification of O-GlcNAcase as a caspase-3 substrate with a novel caspase-3 cleavage site and provide insight about O-GlcNAcase regulation during apoptosis... - O-GlcNAc regulates FoxO activation in response to glucoseMichael P Housley
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 283:16283-92. 2008..GlcNAcylation of FoxO provides a new mechanism for direct nutrient control of transcription to regulate metabolism and stress response through control of FoxO1 activity... - Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resistance and glucose toxicityRonald J Copeland
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
Am J Physiol Endocrinol Metab 295:E17-28. 2008.... - Regulation of the O-linked beta-N-acetylglucosamine transferase by insulin signalingStephen A Whelan
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
J Biol Chem 283:21411-7. 2008..We conclude that insulin stimulates the tyrosine phosphorylation and activity of OGT... - O-GlcNAc modification of nucleocytoplasmic proteins and diabetesYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, 181 8611, Japan
Med Mol Morphol 38:84-91. 2005..In this article, we review the current data regarding the relationship between O-GlcNAc modification and diabetes... - O-GlcNAcylation regulates phosphorylation of tau: a mechanism involved in Alzheimer's diseaseFei Liu
Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA
Proc Natl Acad Sci U S A 101:10804-9. 2004.... - Posttranslational, reversible O-glycosylation is stimulated by high glucose and mediates plasminogen activator inhibitor-1 gene expression and Sp1 transcriptional activity in glomerular mesangial cellsHoward J Goldberg
Department of Medicine, Mount Sinai Hospital and University Health Network, Toronto, Ontario, Canada M5G 1X5
Endocrinology 147:222-31. 2006..These findings demonstrate for the first time that among the pathways served by the HBP, O-GlcNAcylation, is obligatory for HG-induced PAI-1 gene expression and Sp1 transcriptional activation in mesangial cells... - Cell signaling, the essential role of O-GlcNAc!Natasha E Zachara
Department of Biological Chemistry, Johns Hopkins Singapore, 31 Biopolis Way, 02-01 The Nanos, 138669 Singapore
Biochim Biophys Acta 1761:599-617. 2006..Recent research has highlighted key roles for O-GlcNAc serving as a nutrient sensor in regulating insulin signaling, the cell cycle, and calcium handling, as well as the cellular stress response... - Localization of the O-GlcNAc transferase and O-GlcNAc-modified proteins in rat cerebellar cortexYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181-8611, Tokyo, Japan
Brain Res 966:194-205. 2003..Thus, by modulating the phosphorylation or protein associations of key regulatory and cytoskeletal proteins, O-GlcNAc is likely important to many functions of the cerebellum... - Elevation of the post-translational modification of proteins by O-linked N-acetylglucosamine leads to deterioration of the glucose-stimulated insulin secretion in the pancreas of diabetic Goto-Kakizaki ratsYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Glycobiology 17:127-40. 2007..These results indicate that elevation of the O-GlcNAcylation of proteins leads to deterioration of insulin secretion in the pancreas of diabetic GK rats, further providing evidence for the role of O-GlcNAc in the insulin secretion... - Cardioprotection by N-acetylglucosamine linkage to cellular proteinsSteven P Jones
Institute of Molecular Cardiology, University of Louisville School of Medicine, Louisville, KY 40202, USA
Circulation 117:1172-82. 2008..Considering this, we hypothesized that augmentation of O-GlcNAc levels represents an endogenously recruitable mechanism of cardioprotection... - Murine platelets are not regulated by O-linked beta-N-acetylglucosamineGarland L Crawford
Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, 741 S Limestone, BBSRB, Lexington, KY 40536, USA
Arch Biochem Biophys 474:220-4. 2008..These data suggest that while the modification occurs in platelets, their activity is not globally sensitive to O-GlcNAc levels... - N-propanoylmannosamine interferes with O-GlcNAc modification of the tyrosine 3-monooxygenase and stimulates dopamine secretionKaya Bork
Charite Universitatsmedizin Berlin, Berlin, Germany
J Neurosci Res 86:647-52. 2008..We therefore propose a model in which the application of ManNProp leads to increased phosphorylation and activation of tyrosine 3-monooxygenase, which in turn leads to an increased synthesis of dopamine...
Research Grants
- Cytosolic O-Glycosylation and Insulin SignalingGerald Hart; Fiscal Year: 2009..These studies will result in novel avenues for therapeutics. ..
- GLYCOSYLATION AND CANCERGerald Warren Hart; Fiscal Year: 2010..This study will reveal the interplay between this sugar and phosphate in the regulation of signaling cascades important to cancer, and will open new avenues for drug development. ..
- GLYCOSYLATION AND CANCERGerald Hart; Fiscal Year: 2007..abstract_text> ..
- GLYCOCONJUGATES IN DEVELOPMENT AND IMMUNITYGerald Hart; Fiscal Year: 2007..These studies are fundamentally important to the regulation of transcription and signal transduction processes important to virtually all aspects of development and disease. ..
- Cytosolic O-Glycosylation and Insulin SignalingGerald Hart; Fiscal Year: 2007..These studies will result in novel avenues for therapeutics. ..
- O-GlcNAc:Developing New Tool for Assessment of GlycemiaGerald Hart; Fiscal Year: 2007..The project also represents a new inter-disciplinary, inter-departmental basic science-clinical research collaboration between Biological Chemistry and The Department of Medicine. ..
- Cytosolic O-Glycosylation and Insulin SignalingGerald Hart; Fiscal Year: 2007..These studies will result in novel avenues for therapeutics. ..
- Cytosolic O-Glycosylation and Insulin SignalingGerald Hart; Fiscal Year: 2004....
- ROLE OF CELL-SURFACE GLYCOSYLATION IN TUMOR METASTASISGerald Hart; Fiscal Year: 1992..These studies are using the best available animal models of tumor metastasis to examine the mechanisms behind and the roles of altered glycosylation in generation of the metastatic phenotype...
- GLYCOSYLATION AND CANCERGerald Hart; Fiscal Year: 2003..Furthermore, the saccharide appears to modulate the transcriptional activities and cellular associations of key transcription factors involved in transformation to the oncogenic phenotype. ..
- Cytosolic O-Glycosylation and Insulin SignalingGerald Warren Hart; Fiscal Year: 2010..Data from these experiments will reveal totally new avenues for developing treatments for diabetes. ..