Joe G N Garcia

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Orthologous gene-expression profiling in multi-species models: search for candidate genes
    Dmitry N Grigoryev
    Center for Translational Respiratory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, East Monument Street, Baltimore, MD 21287, USA
    Genome Biol 5:R34. 2004
  2. pmc Science review: searching for gene candidates in acute lung injury
    Dmitry N Grigoryev
    Center for Translational Respiratory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Crit Care 8:440-7. 2004
  3. pmc In vitro identification and in silico utilization of interspecies sequence similarities using GeneChip technology
    Dmitry N Grigoryev
    Division of Pulmonary and Critical Care Medicine, Center for Translational Respiratory Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    BMC Genomics 6:62. 2005
  4. pmc Searching for candidate genes in acute lung injury: SNPs, Chips and PBEF
    Joe G N Garcia
    Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, 4B 77, Baltimore, MD 21224 6801, USA
    Trans Am Clin Climatol Assoc 116:205-19; discussion 220. 2005
  5. ncbi Pertussis toxin directly activates endothelial cell p42/p44 MAP kinases via a novel signaling pathway
    J G Garcia
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Cell Physiol 280:C1233-41. 2001
  6. ncbi Simvastatin attenuates vascular leak and inflammation in murine inflammatory lung injury
    Jeffrey R Jacobson
    Center for Translational Respiratory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Physiol Lung Cell Mol Physiol 288:L1026-32. 2005
  7. ncbi Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability
    Joe G N Garcia
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    FASEB J 16:1064-76. 2002
  8. pmc Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement
    J G Garcia
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Clin Invest 108:689-701. 2001
  9. ncbi Pulmonary endothelial cell barrier enhancement by sphingosine 1-phosphate: roles for cortactin and myosin light chain kinase
    Steven M Dudek
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:24692-700. 2004
  10. ncbi Pre-B-cell-colony-enhancing factor is critically involved in thrombin-induced lung endothelial cell barrier dysregulation
    Shui Q Ye
    Department of Medicine, Section of Pulmonary Critical Care, University of Chicago Pritzker School of Medicine, 5841 S Maryland Avenue, MC 6076, Chicago, IL 60637, USA
    Microvasc Res 70:142-51. 2005

Collaborators

Detail Information

Publications99

  1. pmc Orthologous gene-expression profiling in multi-species models: search for candidate genes
    Dmitry N Grigoryev
    Center for Translational Respiratory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, East Monument Street, Baltimore, MD 21287, USA
    Genome Biol 5:R34. 2004
    ..The results suggest that this approach may be a useful tool in the evaluation of biological processes of interest and selection of process-related candidate genes...
  2. pmc Science review: searching for gene candidates in acute lung injury
    Dmitry N Grigoryev
    Center for Translational Respiratory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Crit Care 8:440-7. 2004
    ..The candidate gene approach coupled with extensive gene profiling and novel bioinformatics approaches is a valuable way to identify genes that are involved in ALI...
  3. pmc In vitro identification and in silico utilization of interspecies sequence similarities using GeneChip technology
    Dmitry N Grigoryev
    Division of Pulmonary and Critical Care Medicine, Center for Translational Respiratory Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    BMC Genomics 6:62. 2005
    ..The Affymetrix platform probe redundancy offers flexibility in selecting individual probes with high sequence similarities between related species for gene expression analysis...
  4. pmc Searching for candidate genes in acute lung injury: SNPs, Chips and PBEF
    Joe G N Garcia
    Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, 4B 77, Baltimore, MD 21224 6801, USA
    Trans Am Clin Climatol Assoc 116:205-19; discussion 220. 2005
    ..These studies demonstrate the successful application of genomic technologies in the identification of novel candidate genes in complex lung disease...
  5. ncbi Pertussis toxin directly activates endothelial cell p42/p44 MAP kinases via a novel signaling pathway
    J G Garcia
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Cell Physiol 280:C1233-41. 2001
    ....
  6. ncbi Simvastatin attenuates vascular leak and inflammation in murine inflammatory lung injury
    Jeffrey R Jacobson
    Center for Translational Respiratory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Physiol Lung Cell Mol Physiol 288:L1026-32. 2005
    ..g., IL-6, Toll-like receptor 4). Together, these findings confirm significant protection by simvastatin on LPS-induced lung vascular leak and inflammation and implicate a potential role for statins in the management of ALI...
  7. ncbi Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability
    Joe G N Garcia
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    FASEB J 16:1064-76. 2002
    ....
  8. pmc Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement
    J G Garcia
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Clin Invest 108:689-701. 2001
    ..Platelet-released Sph-1-P, linked to Rac- and Rho-dependent cytoskeletal rearrangement, may act late in angiogenesis to stabilize newly formed vessels, which often display abnormally increased vascular permeability...
  9. ncbi Pulmonary endothelial cell barrier enhancement by sphingosine 1-phosphate: roles for cortactin and myosin light chain kinase
    Steven M Dudek
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:24692-700. 2004
    ..These data support novel roles for the cytoskeletal proteins cortactin and EC MLCK in mediating lung vascular barrier augmentation evoked by S1P...
  10. ncbi Pre-B-cell-colony-enhancing factor is critically involved in thrombin-induced lung endothelial cell barrier dysregulation
    Shui Q Ye
    Department of Medicine, Section of Pulmonary Critical Care, University of Chicago Pritzker School of Medicine, 5841 S Maryland Avenue, MC 6076, Chicago, IL 60637, USA
    Microvasc Res 70:142-51. 2005
    ..Ongoing studies will continue to address the molecular mechanisms by which PBEF contributes to ALI susceptibility...
  11. pmc Differential involvement of ezrin/radixin/moesin proteins in sphingosine 1-phosphate-induced human pulmonary endothelial cell barrier enhancement
    Djanybek M Adyshev
    Institute for Personalized Respiratory Medicine, Department of Medicine, Section of Pulmonary, Critical Care, Sleep, and Allergy, University of Illinois at Chicago, Chicago, IL 60612, USA
    Cell Signal 23:2086-96. 2011
    ..These results suggest that ERM activation is an important regulatory event in EC barrier responses to S1P...
  12. ncbi Differential regulation of human lung epithelial and endothelial barrier function by thrombin
    Kamon Kawkitinarong
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Respir Cell Mol Biol 31:517-27. 2004
    ....
  13. ncbi Signaling pathways involved in adenosine triphosphate-induced endothelial cell barrier enhancement
    Irina A Kolosova
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Circ Res 97:115-24. 2005
    ..We conclude that the mechanisms of ATP-induced barrier enhancement are independent of intracellular Ca2+, but involve activation of myosin phosphatase via a novel G-protein-coupled mechanism and PKA...
  14. ncbi Activated protein C mediates novel lung endothelial barrier enhancement: role of sphingosine 1-phosphate receptor transactivation
    James H Finigan
    Center for Translational Respiratory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland 21224, USA
    J Biol Chem 280:17286-93. 2005
    ..These data suggest that novel EPCR ligation and S1P1 transactivation results in EC cytoskeletal rearrangement and barrier protection, components potentially critical to the improved survival of APC-treated patients with severe sepsis...
  15. ncbi Involvement of microtubules and Rho pathway in TGF-beta1-induced lung vascular barrier dysfunction
    Anna A Birukova
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    J Cell Physiol 204:934-47. 2005
    ..These results suggest a novel role for MT dynamics in the TGF-beta1-mediated Rho regulation, EC barrier dysfunction, and actin remodeling...
  16. pmc Dynamin 2 and c-Abl are novel regulators of hyperoxia-mediated NADPH oxidase activation and reactive oxygen species production in caveolin-enriched microdomains of the endothelium
    Patrick A Singleton
    Department of Medicine, Pritzker School of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 284:34964-75. 2009
    ..These results suggest that hyperoxia induces c-Abl-mediated dynamin 2 phosphorylation required for recruitment of p47(phox) to CEMs and subsequent ROS production in lung endothelium...
  17. ncbi Microtubule disassembly induces cytoskeletal remodeling and lung vascular barrier dysfunction: role of Rho-dependent mechanisms
    Anna A Birukova
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Cell Physiol 201:55-70. 2004
    ....
  18. ncbi Regulation of sphingosine 1-phosphate-induced endothelial cytoskeletal rearrangement and barrier enhancement by S1P1 receptor, PI3 kinase, Tiam1/Rac1, and alpha-actinin
    Patrick A Singleton
    Division of Pulmonary and Critical Care Medicine, Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    FASEB J 19:1646-56. 2005
    ....
  19. pmc Functional variants of the sphingosine-1-phosphate receptor 1 gene associate with asthma susceptibility
    Xiaoguang Sun
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill, USA
    J Allergy Clin Immunol 126:241-9, 249.e1-3. 2010
    ..Increased permeability of the microvasculature is a feature of asthma, and the sphingosine-1-phosphate receptor (S1PR1) is an essential participant regulating lung vascular integrity and responses to lung inflammation...
  20. pmc Phospholipase D-mediated activation of IQGAP1 through Rac1 regulates hyperoxia-induced p47phox translocation and reactive oxygen species generation in lung endothelial cells
    Peter V Usatyuk
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 284:15339-52. 2009
    ..These results demonstrate a role of PLD in hyperoxia-mediated IQGAP1 activation through Rac1 in tyrosine phosphorylation of Src and cortactin, as well as in p47(phox) translocation and ROS formation in human lung endothelial cells...
  21. pmc Particulate matter disrupts human lung endothelial barrier integrity via ROS- and p38 MAPK-dependent pathways
    Ting Wang
    Department of Medicine, University of Chicago Pritzker School of Medicine, 5841 S Maryland Avenue, W604, Chicago, IL 60637, USA
    Am J Respir Cell Mol Biol 42:442-9. 2010
    ..These findings support a novel mechanism for PM-induced lung dysfunction and adverse cardiopulmonary outcomes...
  22. ncbi Cytoskeletal activation and altered gene expression in endothelial barrier regulation by simvastatin
    Jeffrey R Jacobson
    Division of Pulmonary and Critical Care, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    Am J Respir Cell Mol Biol 30:662-70. 2004
    ..These novel observations have broad clinical implications in numerous vascular pathobiologies characterized by alterations in vascular integrity including inflammation, angiogenesis, and acute lung injury...
  23. ncbi Epoxycyclopentenone-containing oxidized phospholipids restore endothelial barrier function via Cdc42 and Rac
    Konstantin G Birukov
    Division of Pulmonary and Critical Care Medicine, Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Circ Res 95:892-901. 2004
    ..Thus, oxidative modification of phospholipids during inflammation leads to the formation of novel regulators that may be critically involved in restoration of vascular barrier function...
  24. pmc Quantitative distribution and colocalization of non-muscle myosin light chain kinase isoforms and cortactin in human lung endothelium
    Mary Brown
    Department of Medicine, Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA
    Microvasc Res 80:75-88. 2010
    ....
  25. pmc Akt-mediated transactivation of the S1P1 receptor in caveolin-enriched microdomains regulates endothelial barrier enhancement by oxidized phospholipids
    Patrick A Singleton
    Department of Medicine, Division of Biomedical Sciences, Section of Pulmonary and Critical Medicine, University of Chicago, Ill 60637, USA
    Circ Res 104:978-86. 2009
    ..Taken together, these results suggest Akt-dependent transactivation of S1P(1) within CEMs is important for OxPAPC-mediated cortical actin rearrangement and EC barrier protection...
  26. ncbi CD44 regulates hepatocyte growth factor-mediated vascular integrity. Role of c-Met, Tiam1/Rac1, dynamin 2, and cortactin
    Patrick A Singleton
    Department of Medicine, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 282:30643-57. 2007
    ..Taken together, these results suggest that CD44 is an important regulator of HGF/c-Met-mediated in vitro and in vivo barrier enhancement, a process with essential involvement of Tiam1, Rac1, dynamin 2, and cortactin...
  27. pmc Ezrin/radixin/moesin proteins differentially regulate endothelial hyperpermeability after thrombin
    Djanybek M Adyshev
    Institute for Personalized Respiratory Medicine, Department of Medicine, Section of Pulmonary, Critical Care, Sleep, and Allergy, University of Illinois at Chicago, COMRB 3154, MC 719, 909 S Wolcott Ave, Chicago, IL 60612, USA
    Am J Physiol Lung Cell Mol Physiol 305:L240-55. 2013
    ..These data suggest that ERM proteins play important differential roles in the thrombin-induced modulation of EC permeability, with moesin promoting barrier dysfunction and radixin opposing it. ..
  28. ncbi Transactivation of sphingosine 1-phosphate receptors is essential for vascular barrier regulation. Novel role for hyaluronan and CD44 receptor family
    Patrick A Singleton
    Department of Medicine, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 281:34381-93. 2006
    ..In summary, HA regulates EC barrier function through novel differential CD44 isoform interaction with S1P receptors, S1P receptor transactivation, and RhoA/Rac1 signaling to the EC cytoskeleton...
  29. ncbi Mechanical stress activates xanthine oxidoreductase through MAP kinase-dependent pathways
    Raja Elie E Abdulnour
    Division of Pulmonary and Critical Care Medicine, Bloomberg School of Public Health, Baltimore, Maryland, USA
    Am J Physiol Lung Cell Mol Physiol 291:L345-53. 2006
    ..We conclude that XOR enzymatic activity is significantly increased by mechanical stress via activation of p38 MAP kinase and ERK and plays a critical role in the pathogenesis of pulmonary edema associated with VILI...
  30. pmc FTY720 inhibits ceramide synthases and up-regulates dihydrosphingosine 1-phosphate formation in human lung endothelial cells
    Evgeny V Berdyshev
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 284:5467-77. 2009
    ..These findings open a novel direction for therapeutic applications of FTY720 that focuses on inhibition of ceramide biosynthesis, ceramide-dependent signaling, and the up-regulation of DHS1P generation in cells...
  31. ncbi Role of CPI-17 in the regulation of endothelial cytoskeleton
    Irina A Kolosova
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 287:L970-80. 2004
    ..Together these data suggest the potential importance of PKC/CPI-17-mediated pathway in histamine-triggered cytoskeletal rearrangements leading to lung microvascular barrier compromise...
  32. ncbi Role of CaM kinase II and ERK activation in thrombin-induced endothelial cell barrier dysfunction
    Talaibek Borbiev
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 285:L43-54. 2003
    ..These results strongly suggest the involvement of CaM kinase II and ERK activities in thrombin-mediated caldesmon phosphorylation and both contractile and barrier regulation...
  33. ncbi Regulation of hyperoxia-induced NADPH oxidase activation in human lung endothelial cells by the actin cytoskeleton and cortactin
    Peter V Usatyuk
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 282:23284-95. 2007
    ..These data demonstrate a novel function for cortactin and actin in hyperoxia-induced activation of NADPH oxidase and ROS generation in human lung endothelial cells...
  34. ncbi Role of sphingosine-1 phosphate in the enhancement of endothelial barrier integrity by platelet-released products
    Kane L Schaphorst
    Division of Pulmonary and Critical Care Medicine and Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224 6801, USA
    Am J Physiol Lung Cell Mol Physiol 285:L258-67. 2003
    ..These results indicate that a major physiologically relevant vascular barrier-protective mediator produced by human platelets is S1P...
  35. pmc Non-muscle myosin light chain kinase isoform is a viable molecular target in acute inflammatory lung injury
    Tamara Mirzapoiazova
    Institute for Personalized and Respiratory Medicine, Department of Medicine, University of Illinois at Chicago, 1705 Polk 305, Chicago, IL 60612, USA
    Am J Respir Cell Mol Biol 44:40-52. 2011
    ..These studies validate nmMLCK as an attractive target for ameliorating the adverse effects of dysregulated lung inflammation...
  36. ncbi Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha-induced NFkappaB activity
    Raj Wadgaonkar
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Cell Biochem 94:351-64. 2005
    ..Together, these novel observations indicate that TNFalpha-induced cytoskeletal rearrangement driven by MLCK activity is necessary for TNFalpha-dependent NFkappaB activation and amplification of pro-survival signals...
  37. ncbi p38 MAP kinase-dependent regulation of endothelial cell permeability
    Talaibek Borbiev
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21224, USA
    Am J Physiol Lung Cell Mol Physiol 287:L911-8. 2004
    ..These results suggest the involvement of p38 MAP kinase activities and caldesmon phosphorylation in the MLCK-independent regulation of thrombin-induced endothelial cell permeability...
  38. ncbi Protein kinase Cdelta mediates lysophosphatidic acid-induced NF-kappaB activation and interleukin-8 secretion in human bronchial epithelial cells
    Rhett Cummings
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Biol Chem 279:41085-94. 2004
    ..These results demonstrate for the first time that LPA is a potent stimulator of IL-8 production in HBEpCs, which involves PKCdelta/NF-kappaB signaling pathways...
  39. ncbi Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury
    Brett A Simon
    Department of Anesthesiology and Critical Medicine, Tower 711, Johns Hopkins Hospital, Baltimore, MD 21287 8711, USA
    Am J Physiol Lung Cell Mol Physiol 291:L851-61. 2006
    ....
  40. pmc Particulate matter air pollution disrupts endothelial cell barrier via calpain-mediated tight junction protein degradation
    Ting Wang
    Institute for Personalized Respiratory Medicine, Section of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, Chicago, IL, USA
    Part Fibre Toxicol 9:35. 2012
    ..The mechanism of PM-mediated pathophysiology remains unknown. However, PM is proinflammatory to the endothelium and increases vascular permeability in vitro and in vivo via ROS generation...
  41. pmc Hydrogen sulfide attenuates particulate matter-induced human lung endothelial barrier disruption via combined reactive oxygen species scavenging and Akt activation
    Ting Wang
    Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA
    Am J Respir Cell Mol Biol 47:491-6. 2012
    ..These data suggest a potentially protective role for H(2)S in PM-induced inflammatory lung injury and vascular hyperpermeability...
  42. pmc Protein kinase C-epsilon regulates sphingosine 1-phosphate-mediated migration of human lung endothelial cells through activation of phospholipase D2, protein kinase C-zeta, and Rac1
    Irina Gorshkova
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 283:11794-806. 2008
    ..Activation of this pathway is necessary to stimulate the migration of lung endothelial cells, a key component of the angiogenic process...
  43. ncbi Involvement of site-specific FAK phosphorylation in sphingosine-1 phosphate- and thrombin-induced focal adhesion remodeling: role of Src and GIT
    Yasushi Shikata
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    FASEB J 17:2240-9. 2003
    ..These observations highlight the differential mechanisms of focal adhesion protein complex remodeling and FAK activation by S1P and thrombin and link differential FA remodeling to EC barrier regulation...
  44. pmc A common cortactin gene variation confers differential susceptibility to severe asthma
    Shwu Fan Ma
    Department of Medicine, University of Chicago, Chicago, Illinois, USA
    Genet Epidemiol 32:757-66. 2008
    ....
  45. pmc Genomic assessment of a multikinase inhibitor, sorafenib, in a rodent model of pulmonary hypertension
    Liliana Moreno-Vinasco
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Physiol Genomics 33:278-91. 2008
    ..In summary, sorafenib represents a novel potential treatment for severe PH with the MAPK cascade a potential canonical target...
  46. ncbi Variation in the myosin light chain kinase gene is associated with development of acute lung injury after major trauma
    Jason D Christie
    Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Crit Care Med 36:2794-800. 2008
    ..We tested the association of MYLK gene variation in the development of acute lung injury in major trauma patients...
  47. ncbi Differential effects of shear stress and cyclic stretch on focal adhesion remodeling, site-specific FAK phosphorylation, and small GTPases in human lung endothelial cells
    Yasushi Shikata
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School of Medicine, 5200 Eastern Avenue, Mason F Lord Building, Center Tower, Room 677, Baltimore, MD 21224, USA
    Exp Cell Res 304:40-9. 2005
    ..Thus, our results suggest common signaling and cytoskeletal mechanisms shared by mechanical and chemical factors involved in EC barrier regulation...
  48. ncbi MAP kinases in lung endothelial permeability induced by microtubule disassembly
    Anna A Birukova
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, MFL Center Tower, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 289:L75-84. 2005
    ..These data suggest a direct link between p38 MAPK activation, remodeling of MT network, and EC barrier regulation...
  49. ncbi Role of Rho GTPases in thrombin-induced lung vascular endothelial cells barrier dysfunction
    Anna A Birukova
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    Microvasc Res 67:64-77. 2004
    ....
  50. pmc Differential effects of sphingosine 1-phosphate receptors on airway and vascular barrier function in the murine lung
    Saad Sammani
    Department of Medicine, Pritzker School of Medicine, University of Chicago, Chicago, Illinois, USA
    Am J Respir Cell Mol Biol 43:394-402. 2010
    ....
  51. pmc Intracellular generation of sphingosine 1-phosphate in human lung endothelial cells: role of lipid phosphate phosphatase-1 and sphingosine kinase 1
    Yutong Zhao
    Department of Medicine, Section of Pulmonary and Critical Care Medicine, University of Chicago, Chicago, IL 60637, USA
    J Biol Chem 282:14165-77. 2007
    ..This involves the conversion of extracellular S1P to Sph by LPP-1, which facilitates Sph uptake, followed by the intracellular conversion of Sph to S1P by SphK1...
  52. pmc Oxidized phospholipids reduce vascular leak and inflammation in rat model of acute lung injury
    Stephanie Nonas
    Division of Pulmonary and Critical Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Respir Crit Care Med 173:1130-8. 2006
    ....
  53. pmc Endothelial permeability is controlled by spatially defined cytoskeletal mechanics: atomic force microscopy force mapping of pulmonary endothelial monolayer
    Anna A Birukova
    Division of Biomedical Sciences, Department of Medicine, Section of Pulmonary and Critical Care Medicine University of Chicago, Chicago, Illinois 60637, USA
    Nanomedicine 5:30-41. 2009
    ..Attenuation of thrombin-induced permeability correlates well with stiffness changes from the cell center to periphery. These results directly link for the first time the patterns of cell stiffness with specific EC permeability responses...
  54. ncbi Magnitude-dependent regulation of pulmonary endothelial cell barrier function by cyclic stretch
    Konstantin G Birukov
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins Univ School of Medicine, 5501 Hopkins Bayview Circle, JHAAC, 5A 42, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 285:L785-97. 2003
    ..Thus our study demonstrates a critical role for the magnitude of CS in regulation of agonist-mediated pulmonary endothelial cell permeability and strongly suggests phenotypic regulation of HPAEC barrier properties by CS...
  55. pmc Essential role of pre-B-cell colony enhancing factor in ventilator-induced lung injury
    Sang Bum Hong
    Department of Medicine, University of Chicago Pritzker School of Medicine, 5841 S Maryland Avenue, W604, Chicago, IL 60637, USA
    Am J Respir Crit Care Med 178:605-17. 2008
    ..We previously demonstrated pre-B-cell colony enhancing factor (PBEF) as a biomarker in sepsis and sepsis-induced acute lung injury (ALI) with genetic variants conferring ALI susceptibility...
  56. ncbi Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure
    Krisztina Tar
    Department of Medicine, Division of Biological Sciences, The University of Chicago, Chicago, Illinois, USA
    J Cell Biochem 98:931-53. 2006
    ..PP2A-mediated dephosphorylation of HSP27 and tau correlated with PP2A-induced preservation of EC cytoskeleton and barrier maintenance. Collectively, our observations clearly demonstrate the crucial role of PP2A in EC barrier protection...
  57. ncbi Pre-B-cell colony-enhancing factor as a potential novel biomarker in acute lung injury
    Shui Q Ye
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, 5200 Eastern Avenue, MFL Building Center Tower 664, Baltimore, MD 21224, USA
    Am J Respir Crit Care Med 171:361-70. 2005
    ..Together, these results strongly indicate that PBEF is a potential novel biomarker in ALI and demonstrate the successful application of robust genomic technologies in the identification of candidate genes in complex lung disease...
  58. ncbi Hepatocyte growth factor enhances endothelial cell barrier function and cortical cytoskeletal rearrangement: potential role of glycogen synthase kinase-3beta
    Feng Liu
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    FASEB J 16:950-62. 2002
    ....
  59. pmc Differential regulation of sphingosine kinases 1 and 2 in lung injury
    Raj Wadgaonkar
    Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
    Am J Physiol Lung Cell Mol Physiol 296:L603-13. 2009
    ..Collectively, our results for the first time demonstrate distinct functional roles of the two SphK isoforms in the regulation of LPS-induced lung injury...
  60. ncbi Molecular cloning and characterization of canine pre-B-cell colony-enhancing factor
    James R McGlothlin
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Mason F Lord Memorial Building, Center Tower, Rm 665, 5200 Eastern Avenue, Baltimore, Maryland 21224, USA
    Biochem Genet 43:127-41. 2005
    ..Isolation of the canine PBEF cDNA and expression of its recombinant protein may provide molecular tools to study the molecular mechanism of ALI in the canine model and to elucidate the potential role of PBEF as an ALI biomarker...
  61. ncbi Novel role of microtubules in thrombin-induced endothelial barrier dysfunction
    Anna A Birukova
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, 5200 Eastern Ave, MFL Center Tower 660, Baltimore, MD 21224, USA
    FASEB J 18:1879-90. 2004
    ..Collectively, our data demonstrate for the first time a critical involvement of MT disassembly in thrombin-induced EC barrier dysfunction and indicate G-protein-dependent mechanisms of thrombin-induced MT alteration...
  62. ncbi Signal transduction pathways activated in human pulmonary endothelial cells by OxPAPC, a bioactive component of oxidized lipoproteins
    Konstantin G Birukov
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    Microvasc Res 67:18-28. 2004
    ....
  63. ncbi The role of caldesmon in the regulation of endothelial cytoskeleton and migration
    Tamara Mirzapoiazova
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Cell Physiol 203:520-8. 2005
    ..These results suggest that CaD is critically involved in the regulation of the actin cytoskeleton and migration in EC, and that p38 MAPK-mediated CaD phosphorylation may be involved in endothelial cytoskeletal remodeling...
  64. pmc Differential regulation of pulmonary endothelial monolayer integrity by varying degrees of cyclic stretch
    Anna A Birukova
    Department of Medicine, Section of Pulmonary and Critical Care Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Am J Pathol 168:1749-61. 2006
    ..These results suggest a critical role for the CS amplitude and the balance between Rac and Rho in mechanochemical regulation of lung EC barrier...
  65. pmc Integrin beta4 attenuates SHP-2 and MAPK signaling and reduces human lung endothelial inflammatory responses
    Weiguo Chen
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Cell Biochem 110:718-24. 2010
    ..Upregulation of integrin beta4 may represent an important element of the anti-inflammatory and vascular-protective properties of statins and provides a novel strategy to limit inflammatory vascular syndromes...
  66. ncbi Endothelial cell barrier enhancement by ATP is mediated by the small GTPase Rac and cortactin
    Jeffrey R Jacobson
    Department of Medicine, Pritzker School of Medicine, University of Chicago, IL 60637, USA
    Am J Physiol Lung Cell Mol Physiol 291:L289-95. 2006
    ..Our results suggest for the first time that ATP-mediated barrier protection is associated with cytoskeletal activation and is dependent on both Rac activation and cortactin...
  67. pmc Macrophage migration inhibitory factor in acute lung injury: expression, biomarker, and associations
    Li Gao
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA
    Transl Res 150:18-29. 2007
    ..These data, generated via functional genomic and genetic approaches, suggest that MIF is a relevant molecular target in ALI...
  68. pmc A transgenic mouse with vascular endothelial over-expression of the non-muscle myosin light chain kinase-2 isoform is susceptible to inflammatory lung injury: role of sexual dimorphism and age
    Jaideep Moitra
    Department of Medicine, University of Chicago, Chicago, IL 60637 1470, USA
    Transl Res 151:141-53. 2008
    ..These data reveal that nmMLCK2 overexpression in the endothelium exacerbates lung injury in vivo in a sexually dimorphic and age-dependent manner...
  69. ncbi Protective effects of sphingosine 1-phosphate in murine endotoxin-induced inflammatory lung injury
    Xinqi Peng
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Respir Crit Care Med 169:1245-51. 2004
    ....
  70. ncbi Sphingosine 1-phosphate reduces vascular leak in murine and canine models of acute lung injury
    Bryan J McVerry
    Division of Pulmonary and Critical Care Medicine, Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Am J Respir Crit Care Med 170:987-93. 2004
    ..Together, these results indicate that Sph 1-P represents a novel therapeutic intervention for the prevention of pulmonary edema related to inflammatory injury and increased vascular permeability...
  71. ncbi In vitro and in vivo modulation of vascular barrier integrity by sphingosine 1-phosphate: mechanistic insights
    Bryan J McVerry
    Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, Division of Pulmonary and Critical Care Medicine, 1830 E Monument Street Room 527, Baltimore, MD 21287, USA
    Cell Signal 17:131-9. 2005
    ..These data support an exciting potential therapeutic role for S1P in vascular barrier enhancement necessary for the treatment of critically ill patients...
  72. ncbi S1P induces FA remodeling in human pulmonary endothelial cells: role of Rac, GIT1, FAK, and paxillin
    Yasushi Shikata
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Appl Physiol 94:1193-203. 2003
    ..These results suggest a role of FA rearrangement in S1P-mediated barrier enhancement via Rac- and GIT-mediated processes...
  73. pmc Activated protein C protects against ventilator-induced pulmonary capillary leak
    James H Finigan
    Division of Pulmonary and Critical Care Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 296:L1002-11. 2009
    ..blood vessel development) and specific gene (e.g., MAPK-associated kinase 2 and integrin-beta(6)) levels. These findings indicate that APC is barrier-protective in VILI and that EPCR is a critical participant in APC-mediated protection...
  74. pmc Use of consomic rats for genomic insights into ventilator-associated lung injury
    Stephanie A Nonas
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Physiol Lung Cell Mol Physiol 293:L292-302. 2007
    ..We speculate that the consomic-microarray-SAM approach is a time- and resource-efficient tool for the genetic dissection of complex diseases including VALI...
  75. ncbi Protein kinase A attenuates endothelial cell barrier dysfunction induced by microtubule disassembly
    Anna A Birukova
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA
    Am J Physiol Lung Cell Mol Physiol 287:L86-93. 2004
    ....
  76. ncbi Attenuation of vascular permeability by methylnaltrexone: role of mOP-R and S1P3 transactivation
    Patrick A Singleton
    Department of Medicine, University of Chicago Pritzker School of Medicine, 5841 S Maryland Avenue, W604, Chicago, IL 60637, USA
    Am J Respir Cell Mol Biol 37:222-31. 2007
    ..These results indicate that MNTX provides barrier protection against edemagenic agonists via inhibition of S1P(3) receptor activation and represents a potentially useful therapeutic agent for syndromes of increased vascular permeability...
  77. pmc Inducible nitric oxide synthase contributes to ventilator-induced lung injury
    Xinqi Peng
    Division of Pulmonary and Critical Care Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Respir Crit Care Med 172:470-9. 2005
    ..Inducible nitric oxide synthase (iNOS) has been implicated in the development of acute lung injury. Recent studies indicate a role for mechanical stress in iNOS and endothelial NOS (eNOS) regulation...
  78. ncbi Phorbol esters increase MLC phosphorylation and actin remodeling in bovine lung endothelium without increased contraction
    Natalia V Bogatcheva
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, MD 21218, USA
    Am J Physiol Lung Cell Mol Physiol 285:L415-26. 2003
    ..Together, these events result in stress fiber destabilization and profound actin rearrangement in bovine endothelium, which may result in the physiological alterations observed in these models...
  79. ncbi The role of the microtubules in tumor necrosis factor-alpha-induced endothelial cell permeability
    Irina Petrache
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Respir Cell Mol Biol 28:574-81. 2003
    ..These results strongly suggest the involvement of microtubule rearrangement in TNF-alpha-induced endothelial cell permeability via p38 mitogen-activated protein kinase activation...
  80. ncbi Central involvement of Rho family GTPases in TNF-alpha-mediated bovine pulmonary endothelial cell apoptosis
    Irina Petrache
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, 1830 Building, Room 527, Baltimore, MD 21224, USA
    Biochem Biophys Res Commun 306:244-9. 2003
    ....
  81. pmc Macrophage migration inhibitory factor governs endothelial cell sensitivity to LPS-induced apoptosis
    Rachel L Damico
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, MD 21224, USA
    Am J Respir Cell Mol Biol 39:77-85. 2008
    ..These results show that MIF can protect vascular endothelial cells from inflammation-associated cell damage...
  82. ncbi Regulation of c-Jun N-terminal kinase and p38 kinase pathways in endothelial cells
    Raj Wadgaonkar
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Respir Cell Mol Biol 31:423-31. 2004
    ..Thus, these inhibitors may play an important role in limiting the inflammatory effects of TNF-alpha and IL-1beta...
  83. pmc Receptor tyrosine kinase inhibitors in rodent pulmonary hypertension
    Liliana Moreno-Vinasco
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, Pritzker School of Medicine, University of Chicago, Chicago, IL, 60637, USA
    Adv Exp Med Biol 661:419-34. 2010
    ..Our studies suggest the PKI sorafenib as a potentially novel treatment for severe PH with the MAPK cascade a potential canonical target profoundly effecting vascular cytoskeletal -rearrangements and remodeling1...
  84. ncbi Phosphatase 2A is involved in endothelial cell microtubule remodeling and barrier regulation
    Krisztina Tar
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Cell Biochem 92:534-46. 2004
    ..These results suggest the involvement of PP2A-mediated tau dephosphorylation in alteration of EC MT structure and highlight the potential importance of PP2A in the regulation of EC the MT cytoskeleton and barrier function...
  85. ncbi Re-evaluation of Evans Blue dye as a marker of albumin clearance in murine models of acute lung injury
    Jaideep Moitra
    Department of Medicine, University of Chicago, Chicago, IL 60637 1470, USA
    Transl Res 150:253-65. 2007
    ..This factor may be of significant utility in animal models of inflammatory injury...
  86. pmc Enhanced interaction between focal adhesion and adherens junction proteins: involvement in sphingosine 1-phosphate-induced endothelial barrier enhancement
    Xiaoguang Sun
    Department of Medicine, Division of Biological Sciences, The University of Chicago, 5841 Maryland Ave, Chicago, IL 60637 1470, USA
    Microvasc Res 77:304-13. 2009
    ..These results demonstrate a mechanism of S1P-induced endothelial barrier enhancement via beta-catenin-linked adherens junction and focal adhesion interaction...
  87. ncbi Functional genomic insights into acute lung injury: role of ventilators and mechanical stress
    Stephanie A Nonas
    Center for Translational Respitory Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA
    Proc Am Thorac Soc 2:188-94. 2005
    ..These studies indicate that the candidate gene approach is a robust strategy to provide novel insights into the genetic basis of ALI, and the identification of potentially novel therapeutic targets...
  88. pmc Regulation of the micromechanical properties of pulmonary endothelium by S1P and thrombin: role of cortactin
    Fernando Teran Arce
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Biophys J 95:886-94. 2008
    ....
  89. pmc Targeted disruption of NeuroD, a proneural basic helix-loop-helix factor, impairs distal lung formation and neuroendocrine morphology in the neonatal lung
    Enid R Neptune
    Division of Pulmonary and Critical Care Medicine, Institute of Genetic Medicine, Howard Hughes Medical Institute, Department of Pathology, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 283:21160-9. 2008
    ..These results support an unanticipated role for NeuroD in the regulation of pulmonary neuroendocrine and alveolar morphogenesis and suggest an intimate connection between the neuroendocrine compartment and distal lung development...
  90. ncbi Bioinformatic identification of novel early stress response genes in rodent models of lung injury
    Shwu Fan Ma
    Cancer for Translational Respiratory Medicine, Gene Expression Profiling Core, Division of Pulmonary and Critical Care Medicine, John Hopkins University School of Medicine, Baltimore, Maryland, USA
    Am J Physiol Lung Cell Mol Physiol 289:L468-77. 2005
    ..We speculate that the ortholog-SAM approach is a useful, time- and resource-efficient tool for identification of candidate genes in a variety of complex disease models such as VILI...
  91. pmc Quantitative proteomics analysis of human endothelial cell membrane rafts: evidence of MARCKS and MRP regulation in the sphingosine 1-phosphate-induced barrier enhancement
    Yurong Guo
    Department of Medicine, Johns Hopkins University, Baltimore, Maryland 21234, USA
    Mol Cell Proteomics 6:689-96. 2007
    ....
  92. ncbi Focal adhesions: paradigm for a signaling nexus
    Lewis H Romer
    Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287 4904, USA
    Circ Res 98:606-16. 2006
    ....
  93. ncbi Pulmonary vascular permeability and ischemic injury in gelsolin-deficient mice
    Patrice M Becker
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    Am J Respir Cell Mol Biol 28:478-84. 2003
    ..Lung wet/dry weight and gross histology following ischemia were comparable in wild-type and gsn-/- animals. These data suggest that gelsolin significantly contributes to maintenance of vascular barrier function in the lung...
  94. ncbi Shear stress-mediated cytoskeletal remodeling and cortactin translocation in pulmonary endothelial cells
    Konstantin G Birukov
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    Am J Respir Cell Mol Biol 26:453-64. 2002
    ..Our results suggest a potential role for cortactin in the SS-induced EC cortical cytoskeletal remodeling and demonstrate a novel mechanism of Rac GTPase-dependent regulation of the pulmonary endothelial cytoskeleton by SS...
  95. pmc Exon-based mapping of microarray probes: recovering differential gene expression signal in underpowered hypoxia experiment
    Dmitry N Grigoryev
    Johns Hopkins University, School of Medicine, Baltimore, MD, USA
    Mol Cell Probes 21:134-9. 2007
    ..This method will facilitate re-evaluation of existing underpowered Affymetrix gene expression profiles...
  96. ncbi Attenuation of chronic hypoxic pulmonary hypertension by simvastatin
    Reda E Girgis
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University, 1830 E Monument, 5th Floor, Baltimore, MD 21205, USA
    Am J Physiol Heart Circ Physiol 285:H938-45. 2003
    ..Enhancement of lung eNOS expression does not appear to be involved in mediating this effect...
  97. ncbi Quantitative analysis of sphingoid base-1-phosphates as bisacetylated derivatives by liquid chromatography-tandem mass spectrometry
    Evgeny V Berdyshev
    Division of Pulmonary Critical Care Medicine, Johns Hopkins University, MFL Building, Center Tower, Room 683, 5200 Eastern Avenue, Baltimore, MD 21224, USA
    Anal Biochem 339:129-36. 2005
    ..6+/-0.2 pmol/ml, respectively. The addition of sphingosine (1 microM) to human pulmonary artery endothelial cells in culture resulted in a more than 20-fold increase in the cellular level of S1P, whereas the level of DHS1P was unchanged...
  98. ncbi Differential gene expression in chronic hypoxic pulmonary hypertension: effect of simvastatin treatment
    Reda E Girgis
    Johns Hopkins University, Baltimore, MD 21205, USA
    Chest 128:579S. 2005
  99. ncbi Polymorphisms in the novel gene acyloxyacyl hydroxylase (AOAH) are associated with asthma and associated phenotypes
    Kathleen C Barnes
    Division of Allergy and Clinical Immunology, Department of Medicine, The Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Allergy Clin Immunol 118:70-7. 2006
    ....