Janice P Evans

Summary

Affiliation: Johns Hopkins Bloomberg School of Public Health
Country: USA

Publications

  1. pmc ADAM2 interactions with mouse eggs and cell lines expressing α4/α9 (ITGA4/ITGA9) integrins: implications for integrin-based adhesion and fertilization
    Ulyana V Desiderio
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 5:e13744. 2010
  2. doi request reprint Sperm-egg interaction
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Annu Rev Physiol 74:477-502. 2012
  3. pmc Egg integrins: back in the game of mammalian fertilization
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe St, Baltimore, MD 21205, USA
    ACS Chem Biol 4:321-3. 2009
  4. ncbi request reprint The state of the union: the cell biology of fertilization
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Nat Cell Biol 4:s57-63. 2002
  5. pmc The spatial and mechanical challenges of female meiosis
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Mol Reprod Dev 78:769-77. 2011
  6. ncbi request reprint The molecular basis of sperm-oocyte membrane interactions during mammalian fertilization
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Hum Reprod Update 8:297-311. 2002
  7. ncbi request reprint Fertilin beta and other ADAMs as integrin ligands: insights into cell adhesion and fertilization
    J P Evans
    Department of Biochemistry and Molecular Biology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 615 N Wolfe St, Room 3606A, Baltimore, MD 21205, USA
    Bioessays 23:628-39. 2001
  8. ncbi request reprint Getting sperm and egg together: things conserved and things diverged
    J P Evans
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    Biol Reprod 63:355-360. 2000
  9. ncbi request reprint Effects of perturbation of cell polarity on molecular markers of sperm-egg binding sites on mouse eggs
    J P Evans
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    Biol Reprod 62:76-84. 2000
  10. ncbi request reprint Sperm disintegrins, egg integrins, and other cell adhesion molecules of mammalian gamete plasma membrane interactions
    J P Evans
    Division of Reproductive Biology, Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD, USA
    Front Biosci 4:D114-31. 1999

Research Grants

Collaborators

Detail Information

Publications34

  1. pmc ADAM2 interactions with mouse eggs and cell lines expressing α4/α9 (ITGA4/ITGA9) integrins: implications for integrin-based adhesion and fertilization
    Ulyana V Desiderio
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 5:e13744. 2010
    ..Having shown that ITGA9 on mouse eggs participates in mouse sperm-egg interactions, we sought to characterize ITGA4/ITGA9-ADAM2 interactions...
  2. doi request reprint Sperm-egg interaction
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Annu Rev Physiol 74:477-502. 2012
    ..Such roles include not only functioning as fusogens, or as adhesion molecules for the opposite gamete, but also functioning through interactions in cis with other proteins to regulate membrane order and functionality...
  3. pmc Egg integrins: back in the game of mammalian fertilization
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe St, Baltimore, MD 21205, USA
    ACS Chem Biol 4:321-3. 2009
    ..Recent data provide insights into the function of egg integrins in mammalian fertilization and address some of the controversies regarding the involvement of these molecules in sperm-egg interaction...
  4. ncbi request reprint The state of the union: the cell biology of fertilization
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Nat Cell Biol 4:s57-63. 2002
    ..Here, we discuss the cell and molecular biology of mammalian fertilization, highlight selected recent breakthroughs and attempt to identify key unanswered questions...
  5. pmc The spatial and mechanical challenges of female meiosis
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Mol Reprod Dev 78:769-77. 2011
    ..Here, consideration is given to how cellular mechanics could contribute to successful mammalian female meiosis, ultimately affecting egg quality and competence to form a healthy embryo...
  6. ncbi request reprint The molecular basis of sperm-oocyte membrane interactions during mammalian fertilization
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Hum Reprod Update 8:297-311. 2002
    ..The clinical relevance of correct gamete membrane interactions is also noted...
  7. ncbi request reprint Fertilin beta and other ADAMs as integrin ligands: insights into cell adhesion and fertilization
    J P Evans
    Department of Biochemistry and Molecular Biology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 615 N Wolfe St, Room 3606A, Baltimore, MD 21205, USA
    Bioessays 23:628-39. 2001
    ..Key issues in cell adhesion that pertain to gametes and fertilization will also be highlighted...
  8. ncbi request reprint Getting sperm and egg together: things conserved and things diverged
    J P Evans
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    Biol Reprod 63:355-360. 2000
    ..Data that were presented are reviewed here and discussed with respect to conserved and divergent features of gamete functions...
  9. ncbi request reprint Effects of perturbation of cell polarity on molecular markers of sperm-egg binding sites on mouse eggs
    J P Evans
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    Biol Reprod 62:76-84. 2000
    ..These results indicate that structural remodeling of the mouse egg plasma membrane is accompanied by molecular remodeling, resulting in the localization or activation of specific molecules in subdomains of the plasma membrane...
  10. ncbi request reprint Sperm disintegrins, egg integrins, and other cell adhesion molecules of mammalian gamete plasma membrane interactions
    J P Evans
    Division of Reproductive Biology, Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD, USA
    Front Biosci 4:D114-31. 1999
    ....
  11. ncbi request reprint Roles of the disintegrin domains of mouse fertilins alpha and beta in fertilization
    J P Evans
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
    Biol Reprod 59:145-52. 1998
    ....
  12. ncbi request reprint CaMKII can participate in but is not sufficient for the establishment of the membrane block to polyspermy in mouse eggs
    Allison J Gardner
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Cell Physiol 212:275-80. 2007
    ....
  13. pmc Immunoglobulin superfamily member IgSF8 (EWI-2) and CD9 in fertilisation: evidence of distinct functions for CD9 and a CD9-associated protein in mammalian sperm-egg interaction
    Amanda I Glazar
    Department of Biochemistry, Division of Reproductive Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolf Street, Baltimore, MD 21205, USA
    Reprod Fertil Dev 21:293-303. 2009
    ..The present study highlights the critical importance of design of IVF experiments for the detection of different effects of experimental manipulations on gamete interactions...
  14. ncbi request reprint Establishment of the mammalian membrane block to polyspermy: evidence for calcium-dependent and -independent regulation
    Allison J Gardner
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Room W3606, 615 N Wolfe St, Maryland, USA
    Reproduction 133:383-93. 2007
    ..The membrane block to polyspermy in mammalian eggs is likely to be the culmination of multiple post-fertilization events that together modify the egg membrane's receptivity to sperm...
  15. ncbi request reprint Analysis of the roles of RGD-binding integrins, alpha(4)/alpha(9) integrins, alpha(6) integrins, and CD9 in the interaction of the fertilin beta (ADAM2) disintegrin domain with the mouse egg membrane
    Xiaoling Zhu
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Biol Reprod 66:1193-202. 2002
    ....
  16. ncbi request reprint Calcium and sperm components in the establishment of the membrane block to polyspermy: studies of ICSI and activation with sperm factor
    Genevieve B Wortzman-Show
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University, Bloomberg School of Public Health, Room W3606, 615 North Wolfe Street, Baltimore, MD 21205, USA
    Mol Hum Reprod 13:557-65. 2007
    ....
  17. pmc Reduction of mouse egg surface integrin alpha9 subunit (ITGA9) reduces the egg's ability to support sperm-egg binding and fusion
    Ulyana Vjugina
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Biol Reprod 80:833-41. 2009
    ..This also shows that careful design of IVF parameters allows detection of deficiencies in gamete interactions...
  18. ncbi request reprint Characterization of the binding of recombinant mouse sperm fertilin beta subunit to mouse eggs: evidence for adhesive activity via an egg beta1 integrin-mediated interaction
    J P Evans
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
    Dev Biol 187:79-93. 1997
    ..These results suggest that a beta1-containing integrin participates in the binding of recombinant fertilin beta-EC to mouse eggs...
  19. ncbi request reprint Characterization of the binding of recombinant mouse sperm fertilin alpha subunit to mouse eggs: evidence for function as a cell adhesion molecule in sperm-egg binding
    J P Evans
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
    Dev Biol 187:94-106. 1997
    ..These data are the first evidence to suggest that fertilin alpha can function as a cell adhesion molecule during fertilization, mediating the binding of sperm and egg plasma membranes...
  20. ncbi request reprint New insights into the molecular basis of mammalian sperm-egg membrane interactions
    Ulyana Vjugina
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    Front Biosci 13:462-76. 2008
    ....
  21. pmc Cortical mechanics and meiosis II completion in mammalian oocytes are mediated by myosin-II and Ezrin-Radixin-Moesin (ERM) proteins
    Stephanie M Larson
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Biol Cell 21:3182-92. 2010
    ..Thus, myosin-II and ERM proteins modulate mechanical properties in oocytes, contributing to cell polarity and to completion of meiosis...
  22. ncbi request reprint Analysis of Mammalian sperm-egg membrane interactions during in vitro fertilization
    Genevieve B Wortzman
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University of Bloomberg School of Public Health, Baltimore, MD, USA
    Methods Mol Biol 341:89-101. 2006
    ..These interactions include cell-cell adhesion and then membrane fusion between the two gametes. This chapter details the experimental methods used to for gamete culture and in vitro fertilization using mouse sperm and eggs...
  23. ncbi request reprint Membrane and cortical abnormalities in post-ovulatory aged eggs: analysis of fertilizability and establishment of the membrane block to polyspermy
    Genevieve B Wortzman
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Mol Hum Reprod 11:1-9. 2005
    ..This analysis of egg membrane function deficiencies provides insights into post-ovulatory ageing and has implications for assisted reproductive technologies...
  24. pmc Lack of tyrosylprotein sulfotransferase-2 activity results in altered sperm-egg interactions and loss of ADAM3 and ADAM6 in epididymal sperm
    Matthew R Marcello
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Biol Chem 286:13060-70. 2011
    ..These data suggest that TPST-2-mediated tyrosine O-sulfation participates in regulating the sperm surface proteome or membrane order, ultimately affecting male fertility...
  25. doi request reprint Ultrastructural analysis of egg membrane abnormalities in post-ovulatory aged eggs
    Diane T Dalo
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Bloomberg School of Public Health, John Hopkins University, Baltimore, Maryland 21205, USA
    Int J Dev Biol 52:535-44. 2008
    ....
  26. ncbi request reprint Analysis of fertilin alpha (ADAM1)-mediated sperm-egg cell adhesion during fertilization and identification of an adhesion-mediating sequence in the disintegrin-like domain
    G E Wong
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe St, Baltimore, MD 21205, USA
    J Biol Chem 276:24937-45. 2001
    ..8, inhibited the binding of recombinant fertilin alpha to eggs in one type of binding assay, suggesting that, under certain conditions, fertilin alpha may interact with a KMC.8-sensitive binding site on the egg plasma membrane...
  27. ncbi request reprint Mouse sperm-egg plasma membrane interactions: analysis of roles of egg integrins and the mouse sperm homologue of PH-30 (fertilin) beta
    J P Evans
    Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    J Cell Sci 108:3267-78. 1995
    ....
  28. pmc Prophase I mouse oocytes are deficient in the ability to respond to fertilization by decreasing membrane receptivity to sperm and establishing a membrane block to polyspermy
    Cassie A Kryzak
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Biol Reprod 89:44. 2013
    ..These data indicate that cortical maturation is a component of cytoplasmic maturation during the oocyte-to-egg transition and that the egg cortex has to be appropriately primed and tuned to be responsive to a fertilizing sperm. ..
  29. ncbi request reprint Identification of key functional amino acids of the mouse fertilin beta (ADAM2) disintegrin loop for cell-cell adhesion during fertilization
    X Zhu
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    J Biol Chem 275:7677-83. 2000
    ....
  30. pmc Multivariate analysis of male reproductive function in Inpp5b-/- mice reveals heterogeneity in defects in fertility, sperm-egg membrane interaction and proteolytic cleavage of sperm ADAMs
    Matthew R Marcello
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe St, Baltimore, MD 21205, USA
    Mol Hum Reprod 16:492-505. 2010
    ..Nevertheless, this work provides detailed analysis of the Inpp5b(-/-) phenotype and also a blueprint for multivariate analysis to examine relationships between molecular characteristics and in vitro and in vivo physiological functions...
  31. ncbi request reprint Mammalian membrane block to polyspermy: new insights into how mammalian eggs prevent fertilisation by multiple sperm
    Allison J Gardner
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Reprod Fertil Dev 18:53-61. 2006
    ....
  32. ncbi request reprint Involvement of calcium signaling and the actin cytoskeleton in the membrane block to polyspermy in mouse eggs
    Beth A McAvey
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    Biol Reprod 67:1342-52. 2002
    ..Furthermore, egg activation-associated increased intracellular calcium levels are necessary but not sufficient to affect postfertilization membrane dynamics that contribute to a membrane block to polyspermy...
  33. pmc Targeted disruption of tyrosylprotein sulfotransferase-2, an enzyme that catalyzes post-translational protein tyrosine O-sulfation, causes male infertility
    Atefeh Borghei
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 281:9423-31. 2006
    ..These data strongly suggest that tyrosine sulfation of unidentified substrate(s) play a crucial role in these processes and document for the first time the critical importance of post-translational tyrosine sulfation in male fertility...
  34. ncbi request reprint BRCA2 deficiency in mice leads to meiotic impairment and infertility
    Shyam K Sharan
    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, 1050 Boyles Street, Frederick, MD 21702, USA
    Development 131:131-42. 2004
    ..Nonetheless, there is marked depletion of germ cells in adult females. These studies provide evidence for key roles of the BRCA2 protein in mammalian gametogenesis and meiotic success...

Research Grants4

  1. CELL ADHESION MOLECULES OF MAMMALIAN FERTILIZATION
    Janice Evans; Fiscal Year: 2007
    ..This research also will also address fundamental questions in cell adhesion biology by examining this interesting, biologically relevant cell-cell interaction event. ..
  2. THE MEMBRANE BLOCK TO POLYSPERMY IN MAMMALIAN EGGS
    Janice Evans; Fiscal Year: 2007
    ....