Michael Edidin

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi Membranes are not just rafts
    Saame Raza Shaikh
    Department of Biology, The Johns Hopkins University, 3400 North Charles Street, Mudd Hall, Baltimore, MD 21218, USA
    Chem Phys Lipids 144:1-3. 2006
  2. ncbi Lipid microdomains in cell surface membranes
    M Edidin
    Biology Department, Johns Hopkins University, MD 21218, USA
    Curr Opin Struct Biol 7:528-32. 1997
  3. ncbi Shrinking patches and slippery rafts: scales of domains in the plasma membrane
    M Edidin
    Biology Dept, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Trends Cell Biol 11:492-6. 2001
  4. ncbi Near-field scanning optical microscopy, a siren call to biology
    M Edidin
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    Traffic 2:797-803. 2001
  5. ncbi Membrane cholesterol, protein phosphorylation, and lipid rafts
    M Edidin
    Department of Biology, Johns Hopkins University, 34th and Charles Streets, Baltimore, MD 21218, USA
    Sci STKE 2001:pe1. 2001
  6. pmc Class I MHC molecules as probes of membrane patchiness: from biophysical measurements to modulation of immune responses
    Michael Edidin
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    Immunol Res 47:265-72. 2010
  7. pmc Switching sides: the actin/membrane lipid connection
    Michael Edidin
    Biology Department, The Johns Hopkins University, Baltimore, MD 21218, USA
    Biophys J 91:3963. 2006
  8. ncbi The state of lipid rafts: from model membranes to cells
    Michael Edidin
    Biology Department, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Annu Rev Biophys Biomol Struct 32:257-83. 2003
  9. ncbi Lipids on the frontier: a century of cell-membrane bilayers
    Michael Edidin
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Nat Rev Mol Cell Biol 4:414-8. 2003
  10. pmc Interaction of Bap31 and MHC class I molecules and their traffic out of the endoplasmic reticulum
    Fumiyoshi Abe
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    J Immunol 182:4776-83. 2009

Research Grants

Collaborators

  • Saame Raza Shaikh
  • Fumiyoshi Abe
  • Mathias Oelke
  • Malarvizhi Durai
  • David R Fooksman
  • Zhiping Li
  • Scheherazade Sadegh-Nasseri
  • Karlo Perica
  • Joan Glick Bieler
  • Sarah Boyle
  • Anne Richter
  • Jonathan Schneck
  • Jonathan P Schneck
  • Tsvetelina Pentcheva
  • Kedar Narayan
  • Frances E Weaver
  • Maya W Everett
  • Yael Lavi
  • John J Ladasky
  • Jeanne Kwik
  • Qing Tang
  • Michaela Niemöller
  • Yen Ling Chiu
  • Leah Sibener
  • Mario Assenmacher
  • Andrés De León Medero
  • Ang Tu
  • Paul W Wiseman
  • David L Kolin
  • Edward M Perkins
  • Gavin E Murphy
  • Sarat K Dalai
  • Sriram Subramaniam
  • Levi A Gheber
  • Hewang Li
  • Malini Seth
  • Steven J Steinberg
  • Leonid Margolis
  • Michael P Sheetz
  • Elias T Spiliotis

Detail Information

Publications28

  1. ncbi Membranes are not just rafts
    Saame Raza Shaikh
    Department of Biology, The Johns Hopkins University, 3400 North Charles Street, Mudd Hall, Baltimore, MD 21218, USA
    Chem Phys Lipids 144:1-3. 2006
    ....
  2. ncbi Lipid microdomains in cell surface membranes
    M Edidin
    Biology Department, Johns Hopkins University, MD 21218, USA
    Curr Opin Struct Biol 7:528-32. 1997
    ..Some progress has also been made in visualizing lipid microdomains within cell membranes. We still await, however, a new model of membrane structure that integrates all experimental results...
  3. ncbi Shrinking patches and slippery rafts: scales of domains in the plasma membrane
    M Edidin
    Biology Dept, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Trends Cell Biol 11:492-6. 2001
    ..Both types of domain are transient on a biological timescale but both could create local conditions that enhance molecular interactions, such as those that occur in receptor-mediated signaling...
  4. ncbi Near-field scanning optical microscopy, a siren call to biology
    M Edidin
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    Traffic 2:797-803. 2001
    ..The most significant of these is control of the distance between near-field aperture and the sample surface. Hence, while NSOM promises much, its application to biology is about where electron microscopy was 40 or 50 years ago...
  5. ncbi Membrane cholesterol, protein phosphorylation, and lipid rafts
    M Edidin
    Department of Biology, Johns Hopkins University, 34th and Charles Streets, Baltimore, MD 21218, USA
    Sci STKE 2001:pe1. 2001
    ....
  6. pmc Class I MHC molecules as probes of membrane patchiness: from biophysical measurements to modulation of immune responses
    Michael Edidin
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    Immunol Res 47:265-72. 2010
    ..This offers the possibility of enhancing or reducing T-cell recognition of targets by altering the clustering of their membrane proteins...
  7. pmc Switching sides: the actin/membrane lipid connection
    Michael Edidin
    Biology Department, The Johns Hopkins University, Baltimore, MD 21218, USA
    Biophys J 91:3963. 2006
  8. ncbi The state of lipid rafts: from model membranes to cells
    Michael Edidin
    Biology Department, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Annu Rev Biophys Biomol Struct 32:257-83. 2003
    ..Recent work converges on very small rafts <10 nm in diameter that may enlarge and stabilize when their constituents are cross-linked...
  9. ncbi Lipids on the frontier: a century of cell-membrane bilayers
    Michael Edidin
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Nat Rev Mol Cell Biol 4:414-8. 2003
    ..Our present picture of cell membranes as lipid bilayers is the legacy of a century's study that concentrated on the lipids and proteins of cell-surface membranes. Recent work is changing the picture and is turning the snapshot into a video...
  10. pmc Interaction of Bap31 and MHC class I molecules and their traffic out of the endoplasmic reticulum
    Fumiyoshi Abe
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    J Immunol 182:4776-83. 2009
    ..Thus, in HeLa cells, Bap31 is involved in the exit of peptide-loaded MHC class I from the ER, and its function is regulated by its interaction with its homologue, Bap29...
  11. pmc Polyunsaturated fatty acids and membrane organization: elucidating mechanisms to balance immunotherapy and susceptibility to infection
    Saame Raza Shaikh
    Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States
    Chem Phys Lipids 153:24-33. 2008
    ..In addition, we propose that mechanistic studies with defined experimental protocols will speed the translation of laboratory studies on PUFAs to the clinic...
  12. pmc Plasma membrane lipid diffusion and composition of sea urchin egg membranes vary with ocean temperature
    Frances E Weaver
    Department of Biology, Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    Chem Phys Lipids 151:62-5. 2008
    ..We found similar trends comparing DiI diffusion in membranes of eggs from 15 degrees C waters with those from 10 degrees C. Our findings establish a new approach for manipulating and studying membrane organization...
  13. pmc Cutting edge: phosphatidylinositol 4,5-bisphosphate concentration at the APC side of the immunological synapse is required for effector T cell function
    David Robert Fooksman
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    J Immunol 182:5179-82. 2009
    ..These findings suggest a new regulatory target for modulating immune function that may be exploited for immune escape by pathogens and tumors...
  14. pmc Clustering class I MHC modulates sensitivity of T cell recognition
    David R Fooksman
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    J Immunol 176:6673-80. 2006
    ..Our results indicate that the lateral organization of MHC class I on the cell surface can modulate the sensitivity of T cell recognition of agonist peptide...
  15. pmc Bap31 enhances the endoplasmic reticulum export and quality control of human class I MHC molecules
    John J Ladasky
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    J Immunol 177:6172-81. 2006
    ..Overexpression of the Bap31 homolog, Bap29, decreases surface class levels in HeLa, indicating that it does not substitute for Bap31...
  16. pmc In vivo test of the vertical phase separation hypothesis: the display of major histocompatibility complex (MHC) class I molecules on membranes of B cells from mice fed high-fat diets
    Saame Raza Shaikh
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Br J Nutr 101:804-9. 2009
    ..MHC class I surface levels on B cells were not affected by the MUFA-rich diet. This study suggests that the membrane vertical phase separation hypothesis may have limited application in a physiologically relevant setting...
  17. ncbi Tapasin increases efficiency of MHC I assembly in the endoplasmic reticulum but does not affect MHC I stability at the cell surface
    Maya W Everett
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    J Immunol 179:7646-52. 2007
    ..We propose that tapasin acts primarily to increase efficiency of assembly of MHC I within the ER...
  18. pmc Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin
    Jeanne Kwik
    Biology Department, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 100:13964-9. 2003
    ..Consistent with this observation, agents that sequester plasma membrane PI(4,5)P2 mimic the effects of cholesterol depletion on actin organization and on lateral mobility...
  19. pmc Magnetic field-induced T cell receptor clustering by nanoparticles enhances T cell activation and stimulates antitumor activity
    Karlo Perica
    Department of Biomedical Engineering, Institute of Cell Engineering, Department of Biology, Department of Pathology, and Departments of Oncology and Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, United States
    ACS Nano 8:2252-60. 2014
    ....
  20. pmc Modulation of MHC binding by lateral association of TCR and coreceptor
    Karlo Perica
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 103:1890-8. 2012
    ..Loss of binding induced by changes in lateral organization of TCR and CD8 may serve as a regulatory mechanism to avoid excessive inflammation and immunopathology in response to aggressive infection...
  21. ncbi Polyunsaturated fatty acids, membrane organization, T cells, and antigen presentation
    Saame Raza Shaikh
    Johns Hopkins University, Baltimore, MD 21218, USA
    Am J Clin Nutr 84:1277-89. 2006
    ..Our data-based models show that membrane modification of both effectors and targets is an important, often overlooked, mechanism of immunomodulation by PUFAs...
  22. ncbi Nanoscale artificial antigen presenting cells for T cell immunotherapy
    Karlo Perica
    Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, USA Departments of Pathology, Oncology, and Medicine Institute of Cell Engineering, Johns Hopkins School of Medicine, Baltimore, MD, USA
    Nanomedicine 10:119-29. 2014
    ..This is the first description of nanoscale aAPC that induce antigen-specific T cell proliferation in vitro and lead to effective T cell stimulation and inhibition of tumor growth in vivo...
  23. pmc Staphylococcal enterotoxin A induces small clusters of HLA-DR1 on B cells
    Kedar Narayan
    Graduate Program in Immunology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    PLoS ONE 4:e6188. 2009
    ..These results present direct visual evidence of SEA-mediated clustering of MHC class II molecules on treated antigen presenting cells, and provide a new structural approach to addressing problems of this nature...
  24. pmc Lowering the barriers to random walks on the cell surface
    Qing Tang
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218 2685, USA
    Biophys J 84:400-7. 2003
    ..Thus there are fewer barriers to lateral diffusion on cytoskeleton mutant MEL cells than on normal MEL cells, but this difference does not strongly affect lateral diffusion on the scales measured here...
  25. ncbi Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation by human leukocyte antigen class I molecules
    Saame Raza Shaikh
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    J Lipid Res 48:127-38. 2007
    ..In addition, PUFA-mediated changes in ER-Golgi trafficking point to a new area of PUFA modulation of immune responses...
  26. pmc Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen
    Sarah Boyle
    Biology Department, The Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 101:L57-9. 2011
    ..Our results offer a general approach to the global analysis of lateral organization and receptor clustering in single cells, and can thus be applied when the cell type of interest is rare...
  27. ncbi Cutting edge: Tapasin is retained in the endoplasmic reticulum by dynamic clustering and exclusion from endoplasmic reticulum exit sites
    Tsvetelina Pentcheva
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Immunol 168:1538-41. 2002
    ..Almost all tapasin molecules were clustered, and these clusters diffused freely in the ER. Tapasin oligomers appear to be retained by the failure of the export machinery to recognize them as cargo...
  28. pmc Dynamic patches of membrane proteins
    Yael Lavi
    Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel
    Biophys J 93:L35-7. 2007
    ..The characteristic lifetime of the patches, extracted from the measurements, is approximately 30 s, in excellent agreement with the predictions of the model...

Research Grants24

  1. MECHANISMS FOR FORMATION OF CELL MEMBRANE DOMAINS
    Michael Edidin; Fiscal Year: 2000
    ..The PI proposes to test his model using cells in which the barriers to lateral mobility are defective, sph/sph, alpha-spectrin-deficient, erythroleukemia cells, and cells in which vesicle traffic from and to the surface is inhibited. ..
  2. CHEMISTRY AND DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 2000
    ....
  3. MECHANISMS FOR FORMATION OF CELL MEMBRANE DOMAINS
    Michael Edidin; Fiscal Year: 2001
    ..The PI proposes to test his model using cells in which the barriers to lateral mobility are defective, sph/sph, alpha-spectrin-deficient, erythroleukemia cells, and cells in which vesicle traffic from and to the surface is inhibited. ..
  4. CHEMISTRY AND DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 2001
    ....
  5. MECHANISMS FOR FORMATION OF CELL MEMBRANE DOMAINS
    Michael Edidin; Fiscal Year: 2002
    ..The PI proposes to test his model using cells in which the barriers to lateral mobility are defective, sph/sph, alpha-spectrin-deficient, erythroleukemia cells, and cells in which vesicle traffic from and to the surface is inhibited. ..
  6. CHEMISTRY AND DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 2002
    ....
  7. CHEMISTRY AND DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 2003
    ..Chaperones and export carriers, tapasin and BAP31 will also be tagged with FP as will viral proteins, the US3 protein of HCMV, and the MCMV proteins m4(p34) and m152. ..
  8. CHEMISTRY AND DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 2006
    ..Chaperones and export carriers, tapasin and BAP31 will also be tagged with FP as will viral proteins, the US3 protein of HCMV, and the MCMV proteins m4(p34) and m152. ..
  9. MECHANISMS FOR FORMATION OF CELL MEMBRANE DOMAINS
    Michael Edidin; Fiscal Year: 1999
    ..The PI proposes to test his model using cells in which the barriers to lateral mobility are defective, sph/sph, alpha-spectrin-deficient, erythroleukemia cells, and cells in which vesicle traffic from and to the surface is inhibited. ..
  10. CHEMISTRY AND DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 1999
    ....
  11. CHEMISTRY & DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 1980
    ....
  12. CHEMISTRY AND DEVELOPMENT OF TRANSPLANTATION ANTIGENS
    Michael Edidin; Fiscal Year: 2007
    ..Chaperones and export carriers, tapasin and BAP31 will also be tagged with FP as will viral proteins, the US3 protein of HCMV, and the MCMV proteins m4(p34) and m152. ..