Stephen J Dumler

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Analysis of genetic identity of North American Anaplasma phagocytophilum strains by pulsed-field gel electrophoresis
    J S Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Clin Microbiol 41:3392-4. 2003
  2. pmc Demonstration of OspC type diversity in invasive human lyme disease isolates and identification of previously uncharacterized epitopes that define the specificity of the OspC murine antibody response
    Christopher G Earnhart
    Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, Richmond, VA 23298 0678, USA
    Infect Immun 73:7869-77. 2005
  3. pmc Lessons from Anaplasma phagocytophilum: chromatin remodeling by bacterial effectors
    Kristen E Rennoll-Bankert
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Infect Disord Drug Targets 12:380-7. 2012
  4. pmc The biological basis of severe outcomes in Anaplasma phagocytophilum infection
    J Stephen Dumler
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    FEMS Immunol Med Microbiol 64:13-20. 2012
  5. pmc Restricted changes in major surface protein-2 (msp2) transcription after prolonged in vitro passage of Anaplasma phagocytophilum
    Diana G Scorpio
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    BMC Microbiol 4:1. 2004
  6. pmc Lymph node hemophagocytosis in rickettsial diseases: a pathogenetic role for CD8 T lymphocytes in human monocytic ehrlichiosis (HME)?
    Kerry L Dierberg
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    BMC Infect Dis 6:121. 2006
  7. pmc A morphological and molecular study of Anaplasma phagocytophilum transmission events at the time of Ixodes ricinus tick bite
    Erik G Granquist
    Department of Production Animal Clinical Sciences, Section of Small Ruminant Research, Norwegian School of Veterinary Science, Sandnes, N 4325 Norway
    Acta Vet Scand 52:43. 2010
  8. pmc Anaplasma phagocytophilum delay of neutrophil apoptosis through the p38 mitogen-activated protein kinase signal pathway
    Kyoung Seong Choi
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Ross 624, Baltimore, MD 21205, USA
    Infect Immun 73:8209-18. 2005
  9. ncbi request reprint Anaplasma and Ehrlichia infection
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Ann N Y Acad Sci 1063:361-73. 2005
  10. ncbi request reprint Molecular methods for ehrlichiosis and Lyme disease
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Room 624, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Clin Lab Med 23:867-84, vi. 2003

Research Grants

  1. Anaplasma regulation of host granulocyte functions
    JOHN DUMLER; Fiscal Year: 2007
  2. Virulence and Immunity of Granulocytic Ehrlichiae
    JOHN DUMLER; Fiscal Year: 2007
  3. Anaplasma regulation of host granulocyte functions
    JOHN DUMLER; Fiscal Year: 2009
  4. Diagnosis of gambiense HAT
    JOHN DUMLER; Fiscal Year: 2009
  5. Virulence and Immunity of Granulocytic Ehrlichiae
    JOHN DUMLER; Fiscal Year: 2005
  6. HUMAN GRANULOCYTIC EHRLICHIA
    JOHN DUMLER; Fiscal Year: 1999
  7. EHRLICHIA-GRANULOCYTE INTERACTIONS AND INFECTION
    JOHN DUMLER; Fiscal Year: 2003
  8. Anaplasma regulation of host granulocyte function
    John Stephen Dumler; Fiscal Year: 2010

Collaborators

Detail Information

Publications54

  1. pmc Analysis of genetic identity of North American Anaplasma phagocytophilum strains by pulsed-field gel electrophoresis
    J S Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Clin Microbiol 41:3392-4. 2003
    ..phagocytophilum strains were compared by PFGE. The average genome size was 1.58 Mbp, and restriction patterns were identical. New World strains of A. phagocytophilum have a large genome and a high degree of genetic uniformity...
  2. pmc Demonstration of OspC type diversity in invasive human lyme disease isolates and identification of previously uncharacterized epitopes that define the specificity of the OspC murine antibody response
    Christopher G Earnhart
    Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, Richmond, VA 23298 0678, USA
    Infect Immun 73:7869-77. 2005
    ..These analyses provide important insight into the antigenic structure of OspC and also provide a basis for understanding the variable nature of the antibody response to this important virulence factor of the Lyme disease spirochetes...
  3. pmc Lessons from Anaplasma phagocytophilum: chromatin remodeling by bacterial effectors
    Kristen E Rennoll-Bankert
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Infect Disord Drug Targets 12:380-7. 2012
    ..This review will focus on how bacterial pathogens alter host epigenetics, by specifically examining A. phagocytophilum AnkA cis-regulation of CYBB transcription and epigenetic changes associated with infection...
  4. pmc The biological basis of severe outcomes in Anaplasma phagocytophilum infection
    J Stephen Dumler
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    FEMS Immunol Med Microbiol 64:13-20. 2012
    ..Whether severity relates to microbial factors or genetically determined diversity in human immune and inflammatory response needs more investigation...
  5. pmc Restricted changes in major surface protein-2 (msp2) transcription after prolonged in vitro passage of Anaplasma phagocytophilum
    Diana G Scorpio
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    BMC Microbiol 4:1. 2004
    ..Thus, Msp2 profiles and msp2 transcripts of low passage A. phagocytophilum Webster strain, initially comprised of a single abundant msp2 transcript, were re-examined after > or = 20 in vitro passages without immune selection...
  6. pmc Lymph node hemophagocytosis in rickettsial diseases: a pathogenetic role for CD8 T lymphocytes in human monocytic ehrlichiosis (HME)?
    Kerry L Dierberg
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    BMC Infect Dis 6:121. 2006
    ..The pathogenesis of RMSF relates to rickettsia-mediated vascular injury, but it is unclear in HME...
  7. pmc A morphological and molecular study of Anaplasma phagocytophilum transmission events at the time of Ixodes ricinus tick bite
    Erik G Granquist
    Department of Production Animal Clinical Sciences, Section of Small Ruminant Research, Norwegian School of Veterinary Science, Sandnes, N 4325 Norway
    Acta Vet Scand 52:43. 2010
    ..Anaplasma phagocytophilum is the causative agent of human granulocytic anaplasmosis (HGA) in humans and tick-borne fever (TBF) in ruminants. The bacterium invades and replicates in phagocytes, especially in polymorphonuclear granulocytes...
  8. pmc Anaplasma phagocytophilum delay of neutrophil apoptosis through the p38 mitogen-activated protein kinase signal pathway
    Kyoung Seong Choi
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Ross 624, Baltimore, MD 21205, USA
    Infect Immun 73:8209-18. 2005
    ..Moreover, active intracellular infection contributes more to the overall delay in apoptosis than do components of heat-killed A. phagocytophilum alone...
  9. ncbi request reprint Anaplasma and Ehrlichia infection
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Ann N Y Acad Sci 1063:361-73. 2005
    ..A firmer basis for the scientific understanding of Anaplasmataceae biology will allow logical and rational approaches toward infection control, prevention, and treatment...
  10. ncbi request reprint Molecular methods for ehrlichiosis and Lyme disease
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Room 624, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Clin Lab Med 23:867-84, vi. 2003
    ..This article reviews molecular techniques that have been developed and are effective in the clinical laboratory for the emerging tick-borne infections, ehrlichiosis and Lyme disease...
  11. pmc Human granulocytic anaplasmosis and Anaplasma phagocytophilum
    J Stephen Dumler
    Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Emerg Infect Dis 11:1828-34. 2005
    ..More study is needed to define the immunology and pathogenetic mechanisms and to understand why severe disease develops in some persons and why some animals become long-term permissive reservoir hosts...
  12. ncbi request reprint Molecular diagnosis of Lyme disease: review and meta-analysis
    J S Dumler
    Department of Pathology, Division of Medical Microbiology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Mol Diagn 6:1-11. 2001
    ..Molecular assays for Lyme disease are best used with other diagnostic methods and only in situations in which the clinical probability of Lyme disease is high...
  13. ncbi request reprint Human granulocytic anaplasmosis and macrophage activation
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Clin Infect Dis 45:199-204. 2007
    ..Macrophage activation and excessive cytokine production may belie tissue injury associated with Ananplasma phagocytophilum infection...
  14. ncbi request reprint Ehrlichioses in humans: epidemiology, clinical presentation, diagnosis, and treatment
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Clin Infect Dis 45:S45-51. 2007
    ....
  15. pmc Fitness and freezing: vector biology and human health
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Clin Invest 120:3087-90. 2010
    ..How an intracellular microbe with a small genome subverts host cell function for survival provides insight into the control of some cellular function programs and underscores how vector biology can have an impact on human health...
  16. ncbi request reprint Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combi
    J S Dumler
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Int J Syst Evol Microbiol 51:2145-65. 2001
    ..nov. and the genus Neorickettsia should be emended to include Neorickettsia (Ehrlichia) risticii comb. nov. and Neorickettsia (Ehrlichia) sennetsu comb. nov...
  17. ncbi request reprint Molecular diagnosis of human granulocytic anaplasmosis
    J Stephen Dumler
    Department of Pathology, The Johns Hopkins Medical Institutions, Ross Research Building, Room 624, 720 Rutland Avenue, Baltimore, MD 21205 USA
    Expert Rev Mol Diagn 4:559-69. 2004
    ....
  18. pmc Dexamethasone-induced cytokine changes associated with diminished disease severity in horses infected with Anaplasma phagocytophilum
    R S Davies
    Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA
    Clin Vaccine Immunol 18:1962-8. 2011
    ..A role for Th1 response and macrophage activation in hematologic derangements elicited by A. phagocytophilum is not supported by these data and remains unproven...
  19. pmc CXCR2 blockade influences Anaplasma phagocytophilum propagation but not histopathology in the mouse model of human granulocytic anaplasmosis
    Diana G Scorpio
    Department of Comparative Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Clin Diagn Lab Immunol 11:963-8. 2004
    ..The results support the hypothesis of bacterial propagation through chemokine induction and confirm that tissue injury is unrelated to A. phagocytophilum tissue load...
  20. pmc Detection of antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis antigens in sera of Korean patients by western immunoblotting and indirect immunofluorescence assays
    Jin Ho Park
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins Medical Institution, Baltimore, Maryland 21287, USA
    Clin Diagn Lab Immunol 10:1059-64. 2003
    ..phagocytophilum and E. chaffeensis protein antigens, respectively. This report strengthens other evidence regarding the presence of A. phagocytophilum and E. chaffeensis infections in humans in South Korea...
  21. ncbi request reprint Simultaneous infection with Borrelia burgdorferi and human granulocytic ehrlichiosis
    William J Moss
    Department of International Health, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205, USA
    Pediatr Infect Dis J 22:91-2. 2003
    ..Early recognition of concurrent Lyme disease and HGE is important because amoxicillin, an antibiotic of choice for young children with early Lyme disease, is ineffective for HGE...
  22. pmc Epigenetic silencing of host cell defense genes enhances intracellular survival of the rickettsial pathogen Anaplasma phagocytophilum
    Jose C Garcia-Garcia
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS Pathog 5:e1000488. 2009
    ..phagocytophilum infection. Bacterial epigenetic regulation of host cell gene transcription could be a general mechanism that enhances intracellular pathogen survival while altering cell function and promoting disease...
  23. pmc Chronic Q fever in the United States
    Petros C Karakousis
    Department of Medicine, Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, MD 21231 1002, USA
    J Clin Microbiol 44:2283-7. 2006
    ..We review all cases of chronic Q fever reported in the United States and discuss important issues pertaining to epidemiology, diagnosis, and management of this disease...
  24. pmc Innate immune response to Anaplasma phagocytophilum contributes to hepatic injury
    Diana G Scorpio
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Department of Molecular and Comparative Pathobiology, 720 Rutland Avenue, Ross 459, Baltimore, Maryland 21205, USA
    Clin Vaccine Immunol 13:806-9. 2006
    ..We show that despite evasion of these host responses, phox, NOS2, TNF, and MyD88 are activated and contribute to inflammation and hepatic injury more than A. phagocytophilum itself...
  25. pmc Diminished adhesion of Anaplasma phagocytophilum-infected neutrophils to endothelial cells is associated with reduced expression of leukocyte surface selectin
    Kyoung Seong Choi
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Infect Immun 71:4586-94. 2003
    ..These observations may help us to understand the modified biological responses, host inflammation, and immune response that occur with A. phagocytophilum infections...
  26. ncbi request reprint Early induction and late abrogation of respiratory burst in A. phagocytophilum-infected neutrophils
    Kyoung Seong Choi
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Ann N Y Acad Sci 990:488-93. 2003
    ..phagocytophilum and other infectious agents in neutrophils. The effect of this may be to generate a limited functional equivalent of chronic granulomatous disease that predisposes to infections by opportunistic pathogens...
  27. pmc Major surface protein 2 of Anaplasma phagocytophilum facilitates adherence to granulocytes
    Jinho Park
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Infect Immun 71:4018-25. 2003
    ..019) in PSGL-1 monoclonal antibody binding to HL-60 cells was elicited with rMsp2. We conclude that Msp2s of A. phagocytophilum are involved in bacterial adhesion to ligands on host myeloid cells before intracellular infection...
  28. pmc Anaplasma phagocytophilum-Borrelia burgdorferi coinfection enhances chemokine, cytokine, and matrix metalloprotease expression by human brain microvascular endothelial cells
    Dennis J Grab
    Department of Pediatrics, Johns Hopkins University School of Medicine, 200 North Wolfe Street, Room 3147, Baltimore, MD 21287, USA
    Clin Vaccine Immunol 14:1420-4. 2007
    ....
  29. pmc Sequential analysis of Anaplasma phagocytophilum msp2 transcription in murine and equine models of human granulocytic anaplasmosis
    Diana G Scorpio
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Clin Vaccine Immunol 15:418-24. 2008
    ..Alteration in transcription of msp2 has no bearing on clinical disease in horses, suggesting the existence of a separate proinflammatory component differentially expressed with changing in vitro passage...
  30. pmc Retrospective clinical and molecular analysis of conditioned laboratory dogs (Canis familiaris) with serologic reactions to Ehrlichia canis, Borrelia burgdorferi, and Rickettsia rickettsii
    Diana G Scorpio
    Department of Molecular and Comparative Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    J Am Assoc Lab Anim Sci 47:23-8. 2008
    ..Exposure to and potential for infection with these bacteria and other pathogens may contribute to blood and tissue alterations that could confound experiments and lead to misinterpretation of data in canine models...
  31. ncbi request reprint Innate immune tissue injury and murine HGA: tissue injury in the murine model of granulocytic anaplasmosis relates to host innate immune response and not pathogen load
    Diana G Scorpio
    Department of Comparative Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Ann N Y Acad Sci 1063:425-8. 2005
    ..Our hypothesis is that the innate immune response to A. phagocytophilum results in inflammatory histopathology, but does not control the pathogen...
  32. pmc Protease activated receptor signaling is required for African trypanosome traversal of human brain microvascular endothelial cells
    Dennis J Grab
    Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS Negl Trop Dis 3:e479. 2009
    ....
  33. ncbi request reprint Role of reactive nitrogen species in development of hepatic injury in a C57bl/6 mouse model of human granulocytic anaplasmosis
    Michelle D Browning
    Department of Comparative Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Comp Med 56:55-62. 2006
    ..Therefore, human and animal patients with HGA likely would benefit from therapy targeting reduced inflammation to supplement anti-infective modalities...
  34. pmc Borrelia burgdorferi, host-derived proteases, and the blood-brain barrier
    Dennis J Grab
    Department of Pediatrics, The Johns Hopkins University School of Medicine, Park Building, Room 256, 600 North Wolfe Street, Baltimore, MD 21287, USA
    Infect Immun 73:1014-22. 2005
    ..Thus, the fibrinolytic system linked by an activation cascade may lead to focal and transient degradation of tight junction proteins that allows B. burgdorferi to invade the CNS...
  35. ncbi request reprint Lyme borreliosis (Lyme disease): molecular and cellular pathobiology and prospects for prevention, diagnosis and treatment
    Paul G Auwaerter
    Division of General Internal Medicine, The Johns Hopkins University School of Medicine, 10753 Falls Road, Suite 325, Lutherville, MD 21093, USA
    Expert Rev Mol Med 6:1-22. 2004
    ..However, further advances in prevention and therapy depend on continued investigation of the ecological risks and improved understanding of the pathobiology of this obligate bacterial parasite...
  36. ncbi request reprint Human monocytic ehrlichiosis
    John H Stone
    Johns Hopkins Vasculitis Center, Division of Rheumatology, Johns Hopkins Bayview Medical Center, Baltimore, MD 21224, USA
    JAMA 292:2263-70. 2004
    ..The patient improved promptly following institution of doxycycline, and his cardiac function returned to normal over a period of 4 months...
  37. pmc Borrelia burgdorferi ospC heterogeneity among human and murine isolates from a defined region of northern Maryland and southern Pennsylvania: lack of correlation with invasive and noninvasive genotypes
    Muneera Y Alghaferi
    W Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    J Clin Microbiol 43:1879-84. 2005
    ..Dissemination in mice and humans by noninvasive ospC types contradicts the ospC invasiveness hypothesis. Alternative genetic markers for B. burgdorferi disseminated disease should be investigated...
  38. pmc Divergent interactions of Ehrlichia chaffeensis- and Anaplasma phagocytophilum-infected leukocytes with endothelial cell barriers
    Jinho Park
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Infect Immun 71:6728-33. 2003
    ..These results support the hypothesis that Anaplasmataceae intracellular infections lead to unique pathogen-specific host cell functional alterations that are likely important for pathogen survival, pathogenesis, and disease induction...
  39. ncbi request reprint Hepatic pathology in human monocytic ehrlichiosis. Ehrlichia chaffeensis infection
    Ann E Sehdev
    Dept of Pathology, Johns Hopkins Medical Institutions, Meyer B1 193, 600 N Wolfe St, Baltimore, MD 21287, USA
    Am J Clin Pathol 119:859-65. 2003
    ..In the absence of infected hepatocytes or biliary epithelial cells, these findings suggest that host inflammatory or immune responses contribute to the liver injury seen in HME...
  40. ncbi request reprint Anaplasma phagocytophilum ligation to toll-like receptor (TLR) 2, but not to TLR4, activates macrophages for nuclear factor-kappa B nuclear translocation
    Kyoung Seong Choi
    Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Infect Dis 189:1921-5. 2004
    ..NF- kappa B was activated only through TLR2, which suggests its role in innate immune induction with A. phagocytophilum infections. The role of innate immunity in human anaplasmosis immunopathology requires more study...
  41. ncbi request reprint Anaplasma phagocytophilum infection reduces expression of phagocytosis-related receptors on neutrophils
    Justin W A Garyu
    Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Ann N Y Acad Sci 1063:416-9. 2005
    ..To address this, we assayed the expression of these receptors after 24 h of A. phagocytophilum infection in human neutrophils...
  42. ncbi request reprint Anaplasma phagocytophilum AnkA binds to granulocyte DNA and nuclear proteins
    Jinho Park
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, 720 Rutland Avenue, Baltimore, Maryland, USA
    Cell Microbiol 6:743-51. 2004
    ..Whether AnkA binding leads to neutrophil functional alterations, and how such alterations might occur will depend upon definitive identification of binding partners and associated metabolic and biochemical pathways...
  43. doi request reprint Emerging and re-emerging tick-transmitted rickettsial and ehrlichial infections
    David H Walker
    Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 0609, USA
    Med Clin North Am 92:1345-61, x. 2008
    ..Globally, many of these bacteria have been named but the genetic differences among them are often small, and many of their clinical manifestations may not be distinguishable diagnostically...
  44. pmc Differential innate immune cell activation and proinflammatory response in Anaplasma phagocytophilum infection
    Kyoung Seong Choi
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Ross 624, Baltimore, MD 21205, USA
    Infect Immun 75:3124-30. 2007
    ..This result supports the concept that NK1.1(+) cells, including NK and NKT cells, are major components in the early pathogenesis of A. phagocytophilum infection...
  45. pmc Two-year evaluation of Borrelia burgdorferi culture and supplemental tests for definitive diagnosis of Lyme disease
    Peggy Coulter
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Clin Microbiol 43:5080-4. 2005
    ..The full battery of tests could be useful for patients with confusing clinical signs or for providing strong laboratory support for clinical studies of Lyme disease...
  46. pmc Defective phagocytosis in Anaplasma phagocytophilum-infected neutrophils
    Justin W A Garyu
    Department of Pathology, Division of Medical Microbiology, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Ross 624, Baltimore, MD 21205, USA
    Infect Immun 73:1187-90. 2005
    ..A. phagocytophilum infection significantly decreased phagocytosis compared to that of controls (P < 0.05). This further impairment of neutrophil function may promote opportunistic infections and exacerbate disease...
  47. pmc Genetic variability and stability of Anaplasma phagocytophila msp2 (p44)
    Karen Caspersen
    Department of Pathology Division of Medical Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Infect Immun 70:1230-4. 2002
    ..The predominant Msp2 pattern in vitro is determined by transcription from a single gene...
  48. ncbi request reprint Human monocytic ehrlichiosis presenting as acute appendicitis during pregnancy
    Ann E Smith Sehdev
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Clin Infect Dis 35:e99-102. 2002
    ..We report a unique case of HME diagnosed in a woman who presented with abdominal pain and acute appendicitis during early pregnancy and whose condition progressively deteriorated to adult respiratory distress syndrome...
  49. ncbi request reprint Rocky Mountain spotted fever--changing ecology and persisting virulence
    J Stephen Dumler
    Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, USA
    N Engl J Med 353:551-3. 2005
  50. ncbi request reprint Anaplasma phagocytophilum major surface protein-2 (Msp2) forms multimeric complexes in the bacterial membrane
    Jinho Park
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Ross Research Building, Room 624, 720 Rutland Avenue, Baltimore, MD 21205, USA
    FEMS Microbiol Lett 227:243-7. 2003
    ..phagocytophilum proteins. Recognition of Msp2 complexes facilitates understanding of A. phagocytophilum-neutrophil adhesion...
  51. pmc Silencing of host cell CYBB gene expression by the nuclear effector AnkA of the intracellular pathogen Anaplasma phagocytophilum
    Jose C Garcia-Garcia
    Johns Hopkins University School of Medicine, Department of Pathology, Baltimore, MD 21205, USA
    Infect Immun 77:2385-91. 2009
    ..These data suggest a mechanism by which intracellular pathogens directly regulate host cell gene expression mediated by nuclear effectors and changes in host chromatin structure...
  52. pmc Anaplasma phagocytophilum infection induces protracted neutrophil degranulation
    Kyoung Seong Choi
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Infect Immun 72:3680-3. 2004
    ..Degranulation-related inflammatory tissue injury may account for some clinical manifestations in human granulocytic anaplasmosis...
  53. ncbi request reprint Diagnosis of Wihipple disease by immunohistochemical analysis: a sensitive and specific method for the detection of Tropheryma whipplei (the Whipple bacillus) in paraffin-embedded tissue
    Blaire L Baisden
    Department of Pathology, The John Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Am J Clin Pathol 118:742-8. 2002
    ..Immunohistochemical analysis is a sensitive and specific method for the diagnosis of Whipple disease in paraffin-embedded tissue and may provide new opportunities to investigate the pathogenesis of the infection...
  54. pmc Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari: Ixodidae) from Madeira Island and Setubal District, mainland Portugal
    Ana Sofia Santos
    Instituto Nacional de Saude Dr Ricardo Jorge, Aguas de Moura, Portugal
    Emerg Infect Dis 10:1643-8. 2004
    ..phagocytophilum in ticks from Setubal District, mainland Portugal, and the first documentation of Anaplasma infection in I. ventalloi. Moreover, these findings confirm the persistence of A. phagocytophilum in Madeira Island's I. ricinus...

Research Grants22

  1. Anaplasma regulation of host granulocyte functions
    JOHN DUMLER; Fiscal Year: 2007
    ..With this will come a strategy for design of prevention, management, and treatment of HGA, and a new model for investigatingthe neutrophil biology. ..
  2. Virulence and Immunity of Granulocytic Ehrlichiae
    JOHN DUMLER; Fiscal Year: 2007
    ..This information will provide a framework for rational design of therapies aimed at modulating immune injury and could foster design of preventative measures aimed at immunization. ..
  3. Anaplasma regulation of host granulocyte functions
    JOHN DUMLER; Fiscal Year: 2009
    ..With this will come a strategy for design of prevention, management, and treatment of HGA, and a new model for investigating the neutrophil biology. ..
  4. Diagnosis of gambiense HAT
    JOHN DUMLER; Fiscal Year: 2009
    ..This is because good tests for diagnosis and staging of sleeping sickness in rural areas do not exist. We want to develop a new and easy test for early diagnosis of this deadly disease. ..
  5. Virulence and Immunity of Granulocytic Ehrlichiae
    JOHN DUMLER; Fiscal Year: 2005
    ..This information will provide the framework for rational design of new therapies aimed at modulating immune mechanisms and design of preventative measures aimed at immunization. ..
  6. HUMAN GRANULOCYTIC EHRLICHIA
    JOHN DUMLER; Fiscal Year: 1999
    ..All of these are imperative to assess the degree of human risk and in developing strategies for control and prevention of this emerging infection ..
  7. EHRLICHIA-GRANULOCYTE INTERACTIONS AND INFECTION
    JOHN DUMLER; Fiscal Year: 2003
    ..phagocytophila group ankyrin protein, EPANK1. ..
  8. Anaplasma regulation of host granulocyte function
    John Stephen Dumler; Fiscal Year: 2010
    ....