M Donowitz

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc SLC9/NHE gene family, a plasma membrane and organellar family of Na⁺/H⁺ exchangers
    Mark Donowitz
    Departments of Medicine and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States
    Mol Aspects Med 34:236-51. 2013
  2. pmc Alterations in the proteome of the NHERF2 knockout mouse jejunal brush border membrane vesicles
    M Donowitz
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2195, USA
    Physiol Genomics 43:674-84. 2011
  3. pmc Alterations in the proteome of the NHERF1 knockout mouse jejunal brush border membrane vesicles
    M Donowitz
    Department of Medicine, Div of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Physiol Genomics 42:200-10. 2010
  4. pmc NHE3 regulatory complexes
    Mark Donowitz
    Johns Hopkins University School of Medicine, 720 Rutland Avenue Baltimore, MD 21205, USA
    J Exp Biol 212:1638-46. 2009
  5. ncbi request reprint Regulatory binding partners and complexes of NHE3
    Mark Donowitz
    Department of Medicine, GI Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Physiol Rev 87:825-72. 2007
  6. pmc NHERF family and NHE3 regulation
    Mark Donowitz
    John Hopkins University School of Medicine, Departments of Medicine and Physiology, Baltimore, MD 21205, USA
    J Physiol 567:3-11. 2005
  7. ncbi request reprint The attrition of young physician-scientists: problems and potential solutions
    Mark Donowitz
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Gastroenterology 132:477-80. 2007
  8. ncbi request reprint Cloning and expression of a rabbit cDNA encoding a serum-activated ethylisopropylamiloride-resistant epithelial Na+/H+ exchanger isoform (NHE-2)
    C M Tse
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 268:11917-24. 1993
  9. ncbi request reprint Basic fibroblast growth factor stimulates surface expression and activity of Na(+)/H(+) exchanger NHE3 via mechanism involving phosphatidylinositol 3-kinase
    A J Janecki
    Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, University of Texas Medical School, Houston, Texas 77030, USA
    J Biol Chem 275:8133-42. 2000
  10. ncbi request reprint cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor
    M Zizak
    Department of Medicine, Gl Division, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 274:24753-8. 1999

Research Grants

Collaborators

Detail Information

Publications51

  1. pmc SLC9/NHE gene family, a plasma membrane and organellar family of Na⁺/H⁺ exchangers
    Mark Donowitz
    Departments of Medicine and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States
    Mol Aspects Med 34:236-51. 2013
    ....
  2. pmc Alterations in the proteome of the NHERF2 knockout mouse jejunal brush border membrane vesicles
    M Donowitz
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2195, USA
    Physiol Genomics 43:674-84. 2011
    ....
  3. pmc Alterations in the proteome of the NHERF1 knockout mouse jejunal brush border membrane vesicles
    M Donowitz
    Department of Medicine, Div of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Physiol Genomics 42:200-10. 2010
    ....
  4. pmc NHE3 regulatory complexes
    Mark Donowitz
    Johns Hopkins University School of Medicine, 720 Rutland Avenue Baltimore, MD 21205, USA
    J Exp Biol 212:1638-46. 2009
    ..This is a review of the domain structure of NHE3 and of the scaffolding function and role in the regulation of NHE3 of the NHE3 C-terminal domain...
  5. ncbi request reprint Regulatory binding partners and complexes of NHE3
    Mark Donowitz
    Department of Medicine, GI Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Physiol Rev 87:825-72. 2007
    ..This review deals with short-term regulation of NHE3 and the identity and function of its recognized interacting partners and the multiprotein complexes in which NHE3 functions...
  6. pmc NHERF family and NHE3 regulation
    Mark Donowitz
    John Hopkins University School of Medicine, Departments of Medicine and Physiology, Baltimore, MD 21205, USA
    J Physiol 567:3-11. 2005
    ..In this case, NHERF2 directly binds cGKII in the brush border to form an NHE3 complex, with cGKII also associating with the BB via its myristoylation...
  7. ncbi request reprint The attrition of young physician-scientists: problems and potential solutions
    Mark Donowitz
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Gastroenterology 132:477-80. 2007
    ..We propose strategies that seek to address this problem and help sustain young physician-scientists at career transition points at which they are most vulnerable to give up...
  8. ncbi request reprint Cloning and expression of a rabbit cDNA encoding a serum-activated ethylisopropylamiloride-resistant epithelial Na+/H+ exchanger isoform (NHE-2)
    C M Tse
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 268:11917-24. 1993
    ..Thus, we conclude that NHE-2 is a candidate to be an apical membrane Na+/H+ exchanger in intestinal and renal epithelial cells...
  9. ncbi request reprint Basic fibroblast growth factor stimulates surface expression and activity of Na(+)/H(+) exchanger NHE3 via mechanism involving phosphatidylinositol 3-kinase
    A J Janecki
    Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, University of Texas Medical School, Houston, Texas 77030, USA
    J Biol Chem 275:8133-42. 2000
    ....
  10. ncbi request reprint cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor
    M Zizak
    Department of Medicine, Gl Division, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 274:24753-8. 1999
    ..This study shows that members of the regulatory factor gene family, NHE-RF and E3KARP, are necessary for cAMP inhibition of NHE3 by allowing NHE3 to be phosphorylated. This inhibition is not dependent on the phosphorylation of NHE-RF...
  11. ncbi request reprint Short-term regulation of NHE3 by EGF and protein kinase C but not protein kinase A involves vesicle trafficking in epithelial cells and fibroblasts
    M Donowitz
    Departments of Physiology and Medicine, Johns Hopkins University School of Medicine, 720 Ruland Avenue, Baltimore, MD 21205 2195, USA
    Ann N Y Acad Sci 915:30-42. 2000
    ....
  12. ncbi request reprint Half-lives of plasma membrane Na(+)/H(+) exchangers NHE1-3: plasma membrane NHE2 has a rapid rate of degradation
    M E Cavet
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Am J Physiol Cell Physiol 281:C2039-48. 2001
    ..These results suggest fundamental differences in the cellular processing and trafficking of NHE2 and NHE3. These differences may underlie the specialized roles that these exchangers play in epithelial cells...
  13. ncbi request reprint Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum versus
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 282:25141-51. 2007
    ....
  14. pmc Na+-H+ exchanger 3 (NHE3) is present in lipid rafts in the rabbit ileal brush border: a role for rafts in trafficking and rapid stimulation of NHE3
    X Li
    Department of Medicine, GI Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2195, USA
    J Physiol 537:537-52. 2001
    ....
  15. pmc cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein
    C H Yun
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 94:3010-5. 1997
    ..Transfection in PS120/NHE3 fibroblasts with either NHERF or E3KARP reconstituted cAMP-induced inhibition of NHE3, resulting in 25-30% inhibition in these cells...
  16. ncbi request reprint Physical and genetic mapping of a human apical epithelial Na+/H+ exchanger (NHE3) isoform to chromosome 5p15.3
    S R Brant
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    Genomics 15:668-72. 1993
    ..Linkage analysis between NHE3 and other markers known to map at 5p15 confirmed the localization of NHE3 to chromosome 5p15.3, making NHE3 the most telomeric gene identified on the short arm of chromosome 5...
  17. ncbi request reprint Two histidine residues in the juxta-membrane cytoplasmic domain of Na+/H+ exchanger isoform 3 (NHE3) determine the set point
    B Cha
    GI Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Membr Biol 191:49-58. 2003
    ..We suggest that His-479 and His-499 are part of the H+ sensor, which is involved in determining the sensitivity to the intracellular H+ concentration and Na+/H+ exchange rate...
  18. ncbi request reprint Constitutively active phosphatidylinositol 3-kinase and AKT are sufficient to stimulate the epithelial Na+/H+ exchanger 3
    W Lee-Kwon
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 276:31296-304. 2001
    ..These results demonstrate that activation of PI 3-kinase or Akt is sufficient to stimulate NHE3 transport activity in PS120/NHE3 cells...
  19. ncbi request reprint Na(+)/H(+) exchanger 3 is in large complexes in the center of the apical surface of proximal tubule-derived OK cells
    S Akhter
    Department of Medicine, Gastrointestinal Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Am J Physiol Cell Physiol 283:C927-40. 2002
    ..In the BB fraction, NHE3 is largely in the detergent-insoluble fraction in lipid rafts and/or in large heterogenous complexes ranging from approximately 400 to approximately 900 kDa...
  20. ncbi request reprint Mammalian Na+/H+ exchanger gene family: structure and function studies
    C H Yun
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Am J Physiol 269:G1-11. 1995
    ..The present review deals with the recent developments in the molecular identification of the Na+/H+ exchanger gene family, the functional characteristics, and the short-term regulation of Na+/H+ exchange at molecular and cellular levels...
  21. ncbi request reprint Human duodenal mucosal brush border Na(+)/H(+) exchangers NHE2 and NHE3 alter net bicarbonate movement
    M Repishti
    Department of Medicine, University of California at San Diego, San Diego, California 92103 8413, USA
    Am J Physiol Gastrointest Liver Physiol 281:G159-63. 2001
    ....
  22. ncbi request reprint Role of lipid rafts in Shiga toxin 1 interaction with the apical surface of Caco-2 cells
    O Kovbasnjuk
    Department of Medicine, Johns Hopkins University, Baltimore, MD, USA
    J Cell Sci 114:4025-31. 2001
    ..Although disruption of lipid rafts by cholesterol depletion did not affect the amount of bound Shiga toxin 1 B-subunit, lipid rafts are necessary for toxin uptake across the apical membrane of Caco-2 cells...
  23. ncbi request reprint Cloning, tissue distribution, and functional analysis of the human Na+/N+ exchanger isoform, NHE3
    S R Brant
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Am J Physiol 269:C198-206. 1995
    ..7 kb, whereas both 6.7- and 8.9-kb bands were expressed nearly equivalently in the other tissues. No NHE3 message was detected in the human heart, lung, liver, skeletal muscle, or pancreas...
  24. pmc Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger
    C M Tse
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    EMBO J 10:1957-67. 1991
    ..The near identity of the basolateral Na+/H+ exchanger and the human Na+/H+ exchanger plus the ubiquitous expression of this message suggests that the ileal basolateral Na+/H+ exchanger is the 'housekeeping' Na+/H+ exchanger...
  25. pmc NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum
    Rakhilya Murtazina
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Am J Physiol Cell Physiol 301:C126-36. 2011
    ....
  26. pmc NHERF1 and NHERF2 are necessary for multiple but usually separate aspects of basal and acute regulation of NHE3 activity
    Rafiquel Sarker
    Gastroenterology and Hepatology Division, Department of Medicine, Johns Hopkins Univ School of Medicine, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 300:C771-82. 2011
    ..cAMP-dependent inhibition of NHE3 activity requires either NHERF1 or NHERF2. Stimulation of NHE3 activity by EGF is NHERF1 dependent...
  27. pmc The NHE3 juxtamembrane cytoplasmic domain directly binds ezrin: dual role in NHE3 trafficking and mobility in the brush border
    Boyoung Cha
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 17:2661-73. 2006
    ....
  28. ncbi request reprint Human Na(+)/H(+) exchanger isoform 6 is found in recycling endosomes of cells, not in mitochondria
    Christopher L Brett
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Am J Physiol Cell Physiol 282:C1031-41. 2002
    ..These results suggest that, like its homologs in yeast and plants, NHE6 is an endosomal Na(+)/H(+) exchanger that may regulate intravesicular pH and volume and contribute to lysosomal biogenesis...
  29. ncbi request reprint Cystic fibrosis transmembrane conductance regulator activation is reduced in the small intestine of Na+/H+ exchanger 3 regulatory factor 1 (NHERF-1)- but Not NHERF-2-deficient mice
    Nellie Broere
    Department of Biochemistry, Erasmus University Medical Center, 3015 GE, Rotterdam, The Netherlands
    J Biol Chem 282:37575-84. 2007
    ....
  30. doi request reprint Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice
    Rochelle Cunningham
    Univ of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
    Am J Physiol Renal Physiol 294:F1001-7. 2008
    ....
  31. ncbi request reprint The lateral mobility of NHE3 on the apical membrane of renal epithelial OK cells is limited by the PDZ domain proteins NHERF1/2, but is dependent on an intact actin cytoskeleton as determined by FRAP
    Boyoung Cha
    Department of Physiology, Gastroenterology Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Sci 117:3353-65. 2004
    ..05 microM, 30 minutes) reduced the NHE3 Mf (21.9+/-4.5%) without altering the Deff. Therefore the actin cytoskeleton, independently of NHERF1/2 binding, is necessary for apical membrane mobility of NHE3...
  32. pmc The glycosphingolipid globotriaosylceramide in the metastatic transformation of colon cancer
    Olga Kovbasnjuk
    Division of Gastroenterology, Department of Medicine, and Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:19087-92. 2005
    ..These data demonstrate the appearance of a subpopulation of Gb(3) containing epithelial cells in the metastatic stage of human colon cancer and suggest their possible role in colon cancer invasiveness...
  33. doi request reprint The epithelial brush border Na+/H+ exchanger NHE3 associates with the actin cytoskeleton by binding to ezrin directly and via PDZ domain-containing Na+/H+ exchanger regulatory factor (NHERF) proteins
    Boyoung Cha
    Department of Medicine, GI Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Clin Exp Pharmacol Physiol 35:863-71. 2008
    ..However, it is dynamic; for example, changing in some cases of signalling. Furthermore, NHERF binding is necessary for lysophosphatidic acid stimulation of NHE3 and inhibition of NHE3 by Ca(2+), cAMP and cGMP...
  34. ncbi request reprint Ca(2+)-dependent inhibition of Na+/H+ exchanger 3 (NHE3) requires an NHE3-E3KARP-alpha-actinin-4 complex for oligomerization and endocytosis
    Jae Ho Kim
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 277:23714-24. 2002
    ..From these results, we propose that elevated Ca(2+) inhibits NHE3 activity through oligomerization and endocytosis of NHE3, which occurs via formation of an NHE3-E3KARP-alpha-actinin-4 complex...
  35. ncbi request reprint Na(+)/H(+ ) exchanger regulatory factor 2 directs parathyroid hormone 1 receptor signalling
    Matthew J Mahon
    Endocrine Unit, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 417:858-61. 2002
    ....
  36. ncbi request reprint Lysophosphatidic acid stimulates brush border Na+/H+ exchanger 3 (NHE3) activity by increasing its exocytosis by an NHE3 kinase A regulatory protein-dependent mechanism
    Whaseon Lee-Kwon
    Department of Physiology, Gastrointestinal Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 278:16494-501. 2003
    ..This is the first demonstration that rapid stimulation of NHE3 activity is dependent on an apical membrane PDZ domain protein...
  37. ncbi request reprint The protein kinase Akt induces epithelial mesenchymal transition and promotes enhanced motility and invasiveness of squamous cell carcinoma lines
    Sylvia Julien Grille
    Developmental Genetics of Melanocytes, UMR 146 CNRS Institut Curie, 91405 Orsay Cedex, France
    Cancer Res 63:2172-8. 2003
    ..These findings suggest that future therapies based on AKT inhibition may complement conventional treatments by controlling tumor cell invasion and metastasis...
  38. ncbi request reprint Ca2+-dependent inhibition of NHE3 requires PKC alpha which binds to E3KARP to decrease surface NHE3 containing plasma membrane complexes
    Whaseon Lee-Kwon
    Johns Hopkins Univ School of Medicine, 925 Ross Research Bldg, 720 Rutland Ave, Baltimore, MD 21205 2195, USA
    Am J Physiol Cell Physiol 285:C1527-36. 2003
    ..These results suggest that PKCalpha is not necessary for the Ca2+-dependent formation of the NHE3 plasma membrane complex, although it is necessary for decreasing the membrane amounts of NHE3, probably by stimulating NHE3 endocytosis...
  39. ncbi request reprint Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiation
    Xshang Li
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Gastroenterology 126:122-35. 2004
    ..However, how NHE3 is regulated by the PI 3-K pathway and the role of Akt2 are poorly defined...
  40. pmc Carbachol regulation of rabbit ileal brush border Na+-H+ exchanger 3 (NHE3) occurs through changes in NHE3 trafficking and complex formation and is Src dependent
    Xuhang Li
    Department of Medicine, GI Division, John Hopkins University School of Medicine, Baltimore, MD 21205 2195, USA
    J Physiol 556:791-804. 2004
    ....
  41. ncbi request reprint NHERF2 increases platelet-derived growth factor-induced proliferation through PI-3-kinase/Akt-, ERK-, and Src family kinase-dependent pathway
    Yong Jung Kang
    Department of Physiology, College of Medicine, Pusan National University, 1Ga, Ami dong, Suh gu, Pusan 602 739, South Korea
    Cell Signal 16:791-800. 2004
    ..These results suggest that NHERF2 stimulates PDGF-induced proliferation by increasing PI-3-kinase/Akt, MEKindependent ERK, and Src family kinase-mediated signaling pathways...
  42. ncbi request reprint Molecular physiology of intestinal Na+/H+ exchange
    Nicholas C Zachos
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    Annu Rev Physiol 67:411-43. 2005
    ....
  43. ncbi request reprint cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein
    Boyoung Cha
    Department of Physiology, GI Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:16642-50. 2005
    ..These results show that NHERF2, acting as a novel protein kinase G-anchoring protein, is required for cGMP inhibition of NHE3 and that cGKII must be bound both to the plasma membrane by its myristoyl anchor and to NHERF2 to inhibit NHE3...
  44. ncbi request reprint Lysophosphatidic acid induces exocytic trafficking of Na(+)/H(+) exchanger 3 by E3KARP-dependent activation of phospholipase C
    Jung Woong Choi
    Department of Physiology and Medicine, Gastrointestinal Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Biochim Biophys Acta 1683:59-68. 2004
    ....
  45. ncbi request reprint Evolutionary origins of eukaryotic sodium/proton exchangers
    Christopher L Brett
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Am J Physiol Cell Physiol 288:C223-39. 2005
    ..These novel observations have allowed us to use comparative biology to predict physiological roles for the nine human NHE paralogs and to propose appropriate model organisms in which to study the unique properties of each NHE subclass...
  46. ncbi request reprint Does the proteome encode organellar pH?
    Christopher L Brett
    Department of Physiology, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    FEBS Lett 580:717-9. 2006
    ..This raises an interesting evolutionary question: did the pI of proteins and the pH of organelles co-evolve to optimize function?..
  47. ncbi request reprint Na+/H+ exchanger NHE3 activity and trafficking are lipid Raft-dependent
    Rakhilya Murtazina
    Departments of Physiology and Medicine, Division of Gastroenterology, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    J Biol Chem 281:17845-55. 2006
    ....
  48. ncbi request reprint Proteome of murine jejunal brush border membrane vesicles
    Mark Donowitz
    J Proteome Res 6:4068-79. 2007
    ..This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine...
  49. doi request reprint Fractionation of subcellular membrane vesicles of epithelial and nonepithelial cells by OptiPrep density gradient ultracentrifugation
    Xuhang Li
    GI Division, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 440:97-110. 2008
    ....
  50. pmc Latrunculin B facilitates Shiga toxin 1 transcellular transcytosis across T84 intestinal epithelial cells
    Irina Maluykova
    Department of Medicine, Division of Gastroenterology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Biochim Biophys Acta 1782:370-7. 2008
    ..Since EHEC attachment to epithelial cells causes an actin rearrangement, this finding may be highly relevant to Stx-induced disease...
  51. ncbi request reprint ClC-5: role in endocytosis in the proximal tubule
    YingHong Wang
    Dept of Physiology, WBSB Rm 208, The Johns Hopkins Univ School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Am J Physiol Renal Physiol 289:F850-62. 2005
    ..Our data from the mutants suggest that defective targeting and trafficking of mutant ClC-5 to the endosomes are a major determinant in the lack of normal endocytosis in Dent's disease...

Research Grants37

  1. Diarrheal Disease -- A Physiologic Approach to Treatment
    Mark Donowitz; Fiscal Year: 2007
    ..We also will test effects of stimulation of NHE3 on its BB mobility by use of fluorescence recovery after photobleaching (FRAP). ..
  2. PDZ Domain Proteins and NHE3 Regulation
    Mark Donowitz; Fiscal Year: 2007
    ..abstract_text> ..
  3. Hopkins Digestive Diseases Basic Research Development Center
    Mark Donowitz; Fiscal Year: 2007
    ..Administrative Operations will provide the communication, financial management and administrative functions of the DDBRDC. ..
  4. BASIC SCIENCE RESEARCH IN DIGESTIVE DISEASE
    Mark Donowitz; Fiscal Year: 2007
    ..Fellows who are thought to be successful in being prepared for investigative careers are helped to obtain bridging support and brought on as junior faculty to allow adequate time for training. ..
  5. Diarrheal Disease: A Physiologic Approach to Treatment
    Mark Donowitz; Fiscal Year: 2009
    ..In addition, the coordinated association with NHE3 of these proteins which all bind to a small area of NHE3, will be examined to determine if together they explain how NHE3 changes its activity during digestion and in diarrhea. ..
  6. Diarrheal Disease: A Physiologic Approach to Treatment
    Mark Donowitz; Fiscal Year: 2010
    ..In addition, the coordinated association with NHE3 of these proteins which all bind to a small area of NHE3, will be examined to determine if together they explain how NHE3 changes its activity during digestion and in diarrhea. ..
  7. NHE3 Regulation and Signaling Complexes
    Chung Ming Tse; Fiscal Year: 2010
    ..We suspect that this will provide insight in how normal Na absorption occurs as well as how Na absorption becomes abnormal in diarrheal diseases. ..
  8. Diarrheal Disease: A Physiologic Approach to Treatment
    Mark Donowitz; Fiscal Year: 2009
    ..In addition, the coordinated association with NHE3 of these proteins which all bind to a small area of NHE3, will be examined to determine if together they explain how NHE3 changes its activity during digestion and in diarrhea. ..
  9. NHE3 Regulation and Signaling Complexes
    Mark Donowitz; Fiscal Year: 2009
    ..We suspect that this will provide insight in how normal Na absorption occurs as well as how Na absorption becomes abnormal in diarrheal diseases. ..
  10. DIARRHEAL DISEASE--A PHYSIOLOGIC APPROACH TO TREATMENT
    Mark Donowitz; Fiscal Year: 1993
    ..II. To direct the biochemical studies in I, the identity of the ileal brush border PL(s) activated by carbachol and/or A23187 and the mechanism of their activation will probed...
  11. DIARRHEAL DISEASE: A PHYSIOLOGIC APPROACH TO TREATMENT
    Mark Donowitz; Fiscal Year: 1990
    ....
  12. DIARRHEAL DISEASE--A PHYSIOLOGIC APPROACH TO TREATMENT
    Mark Donowitz; Fiscal Year: 1992
    ....
  13. DIARRHEAL DISEASE--A PHYSIOLOGIC APPROACH TO TREATMENT
    Mark Donowitz; Fiscal Year: 2002
    ....
  14. Diarrheal Disease: A Physiologic Approach to Treatment
    Mark Donowitz; Fiscal Year: 2010
    ..In addition, the coordinated association with NHE3 of these proteins which all bind to a small area of NHE3, will be examined to determine if together they explain how NHE3 changes its activity during digestion and in diarrhea. ..