Pierre A Coulombe

Summary

Affiliation: Johns Hopkins Bloomberg School of Public Health
Country: USA

Publications

  1. pmc Defining keratin protein function in skin epithelia: epidermolysis bullosa simplex and its aftermath
    Pierre A Coulombe
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Invest Dermatol 132:763-75. 2012
  2. pmc Epidermolysis bullosa simplex: a paradigm for disorders of tissue fragility
    Pierre A Coulombe
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    J Clin Invest 119:1784-93. 2009
  3. pmc Exploiting the keratin 17 gene promoter to visualize live cells in epithelial appendages of mice
    Nicholas Bianchi
    Predoctoral Program in Human Genetics, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 212105, USA
    Mol Cell Biol 25:7249-59. 2005
  4. pmc Defining the properties of the nonhelical tail domain in type II keratin 5: insight from a bullous disease-causing mutation
    Li Hong Gu
    Departments of Biological Chemistry and Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 16:1427-38. 2005
  5. pmc Overcoming functional redundancy to elicit pachyonychia congenita-like nail lesions in transgenic mice
    Pauline Wong
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe St, Baltimore, MD 21205, USA
    Mol Cell Biol 25:197-205. 2005
  6. pmc Keratin 17 promotes epithelial proliferation and tumor growth by polarizing the immune response in skin
    Daryle DePianto
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA
    Nat Genet 42:910-4. 2010
  7. pmc Hairless triggers reactivation of hair growth by promoting Wnt signaling
    Gerard M J Beaudoin
    Kennedy Krieger Research Institute and Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:14653-8. 2005
  8. ncbi request reprint The co-repressor hairless has a role in epithelial cell differentiation in the skin
    Joanna M Zarach
    Kennedy Krieger Research Institute, Baltimore, MD 21205, USA
    Development 131:4189-200. 2004
  9. pmc Differential modulation of keratin expression by sulforaphane occurs via Nrf2-dependent and -independent pathways in skin epithelia
    Michelle Kerns
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Biol Cell 21:4068-75. 2010
  10. ncbi request reprint Keratin expression provides novel insight into the morphogenesis and function of the companion layer in hair follicles
    Li Hong Gu
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Invest Dermatol 127:1061-73. 2007

Collaborators

Detail Information

Publications47

  1. pmc Defining keratin protein function in skin epithelia: epidermolysis bullosa simplex and its aftermath
    Pierre A Coulombe
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Invest Dermatol 132:763-75. 2012
    ....
  2. pmc Epidermolysis bullosa simplex: a paradigm for disorders of tissue fragility
    Pierre A Coulombe
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    J Clin Invest 119:1784-93. 2009
    ..Here we review how laboratory investigations centered on keratin biology have deepened our understanding of the etiology and pathophysiology of EB simplex and revealed novel avenues for its therapy...
  3. pmc Exploiting the keratin 17 gene promoter to visualize live cells in epithelial appendages of mice
    Nicholas Bianchi
    Predoctoral Program in Human Genetics, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 212105, USA
    Mol Cell Biol 25:7249-59. 2005
    ..The compact mK17 gene promoter provides a novel tool for appendage-preferred gene expression and manipulation in transgenic mice...
  4. pmc Defining the properties of the nonhelical tail domain in type II keratin 5: insight from a bullous disease-causing mutation
    Li Hong Gu
    Departments of Biological Chemistry and Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 16:1427-38. 2005
    ..These data provide a mechanistic basis for the cell fragility seen in individuals bearing the K5-1649delG allele, and point to the role of the C-terminal 41 residues in determining K5's assembly properties...
  5. pmc Overcoming functional redundancy to elicit pachyonychia congenita-like nail lesions in transgenic mice
    Pauline Wong
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe St, Baltimore, MD 21205, USA
    Mol Cell Biol 25:197-205. 2005
    ..Our findings point to significant redundancy among the multiple keratins expressed in hair and nail, which can be related to the common ancestry, clustered organization, and sequence relatedness of specific keratin genes...
  6. pmc Keratin 17 promotes epithelial proliferation and tumor growth by polarizing the immune response in skin
    Daryle DePianto
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA
    Nat Genet 42:910-4. 2010
    ..Our findings establish an immunomodulatory role for Krt17 in Hh driven basaloid skin tumors that could impact additional tumor settings, psoriasis and wound repair...
  7. pmc Hairless triggers reactivation of hair growth by promoting Wnt signaling
    Gerard M J Beaudoin
    Kennedy Krieger Research Institute and Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:14653-8. 2005
    ..This work links HR and Wnt function, providing a model in which HR regulates the precise timing of Wnt signaling required for hair follicle regeneration...
  8. ncbi request reprint The co-repressor hairless has a role in epithelial cell differentiation in the skin
    Joanna M Zarach
    Kennedy Krieger Research Institute, Baltimore, MD 21205, USA
    Development 131:4189-200. 2004
    ....
  9. pmc Differential modulation of keratin expression by sulforaphane occurs via Nrf2-dependent and -independent pathways in skin epithelia
    Michelle Kerns
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Biol Cell 21:4068-75. 2010
    ....
  10. ncbi request reprint Keratin expression provides novel insight into the morphogenesis and function of the companion layer in hair follicles
    Li Hong Gu
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Invest Dermatol 127:1061-73. 2007
    ..These findings provide novel insight into the morphogenesis and properties of the Cl, and raise the distinct possibility that it is an integral part of the IRS compartment...
  11. pmc Loss of keratin 6 (K6) proteins reveals a function for intermediate filaments during wound repair
    Pauline Wong
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biol 163:327-37. 2003
    ....
  12. ncbi request reprint Skin: an ideal model system to study keratin genes and proteins
    Kelsie M Bernot
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Methods Cell Biol 78:453-87. 2004
  13. ncbi request reprint Keratin function in skin epithelia: a broadening palette with surprising shades
    Li Hong Gu
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Curr Opin Cell Biol 19:13-23. 2007
    ..Other studies extended the role of keratin proteins in regulating the response to pro-apoptotic signals, and revealed their ability to modulate protein synthesis and cell size in epithelial cells challenged to grow...
  14. pmc A wound-induced keratin inhibits Src activity during keratinocyte migration and tissue repair
    Jeremy D Rotty
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21202, USA
    J Cell Biol 197:381-9. 2012
    ..We conclude that K6 negatively regulates Src kinase activity and the migratory potential of skin keratinocytes during wound repair. Our findings may also be important in related contexts such as cancer...
  15. pmc Keratin 17 modulates hair follicle cycling in a TNFalpha-dependent fashion
    Xuemei Tong
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Genes Dev 20:1353-64. 2006
    ..These findings identify K17 and TNFalpha as two novel and interdependent regulators of hair cycling...
  16. pmc Reprogramming of keratin biosynthesis by sulforaphane restores skin integrity in epidermolysis bullosa simplex
    Michelle L Kerns
    Department of Biological Chemistry, 725 North Wolfe Street, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:14460-5. 2007
    ..Nrf2 induction is brought about by treatment with sulforaphane, a natural product. Sulforaphane thus represents an attractive option for the prevention of skin blistering associated with K14 mutations in EBS...
  17. ncbi request reprint Type II epithelial keratin 6hf (K6hf) is expressed in the companion layer, matrix, and medulla in anagen-stage hair follicles
    Zhiling Wang
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Invest Dermatol 121:1276-82. 2003
    ..They also have obvious implications for the epithelial alterations associated with defects in keratin 6 genes...
  18. pmc A small surface hydrophobic stripe in the coiled-coil domain of type I keratins mediates tetramer stability
    Kelsie M Bernot
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biol 168:965-74. 2005
    ....
  19. ncbi request reprint Great promises yet to be fulfilled: defining keratin intermediate filament function in vivo
    Pierre A Coulombe
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Eur J Cell Biol 83:735-46. 2004
    ..Still, detailed studies of the phenotype exhibited by K6 and K17 null mice yielded novel insight into the properties and function of keratin IFs in vivo...
  20. ncbi request reprint Intermediate filament scaffolds fulfill mechanical, organizational, and signaling functions in the cytoplasm
    Seyun Kim
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Genes Dev 21:1581-97. 2007
    ..As this discovery process continues to unfold, a rationale for the large size of this family and the context-dependent regulation of its members is finally emerging...
  21. pmc Overexpressed transient receptor potential vanilloid 3 ion channels in skin keratinocytes modulate pain sensitivity via prostaglandin E2
    Susan M Huang
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21025, USA
    J Neurosci 28:13727-37. 2008
    ..Our results reveal a previously undescribed mechanism for keratinocyte participation in thermal pain transduction through keratinocyte TRPV3 ion channels and the intercellular messenger PGE(2)...
  22. pmc The expanding significance of keratin intermediate filaments in normal and diseased epithelia
    Xiaoou Pan
    Dept of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA
    Curr Opin Cell Biol 25:47-56. 2013
    ....
  23. ncbi request reprint Role for keratins 6 and 17 during wound closure in embryonic mouse skin
    Stacy Mazzalupo
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Dev Dyn 226:356-65. 2003
    ....
  24. ncbi request reprint A keratin cytoskeletal protein regulates protein synthesis and epithelial cell growth
    Seyun Kim
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nature 441:362-5. 2006
    ..These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis...
  25. ncbi request reprint A novel mouse type I intermediate filament gene, keratin 17n (K17n), exhibits preferred expression in nail tissue
    Xuemei Tong
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Invest Dermatol 122:965-70. 2004
    ..Depending on the existence of a human ortholog or a functional equivalent, our findings may also provide a molecular explanation for several unusual aspects of hK17-based diseases...
  26. pmc Hedgehog signaling, keratin 6 induction, and sebaceous gland morphogenesis: implications for pachyonychia congenita and related conditions
    Li Hong Gu
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Am J Pathol 173:752-61. 2008
    ....
  27. ncbi request reprint Interaction between the keratin cytoskeleton and eEF1Bgamma affects protein synthesis in epithelial cells
    Seyun Kim
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, Maryland 21205, USA
    Nat Struct Mol Biol 14:982-3. 2007
    ..These findings extend the emerging relationship between keratin proteins and the translational machinery...
  28. pmc Networking galore: intermediate filaments and cell migration
    Byung Min Chung
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA
    Curr Opin Cell Biol 25:600-12. 2013
    ....
  29. pmc Mathematical modeling of the impact of actin and keratin filaments on keratinocyte cell spreading
    Jin Seob Kim
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 103:1828-38. 2012
    ..These in silico findings provide further support for the notion that differentiation-related changes in the density and intracellular organization of keratin IFs affect tissue architecture in epidermis and related stratified epithelia...
  30. pmc Type I keratin 17 protein is phosphorylated on serine 44 by p90 ribosomal protein S6 kinase 1 (RSK1) in a growth- and stress-dependent fashion
    Xiaoou Pan
    Department of Biochemistry and Molecular Biology, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 286:42403-13. 2011
    ..These findings confirm and expand the tight link that has emerged between K17 up-regulation and growth and stress responses in the skin epithelium...
  31. pmc Self-organization of keratin intermediate filaments into cross-linked networks
    Chang Hun Lee
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    J Cell Biol 186:409-21. 2009
    ..We propose that self-organization is a key determinant of the structural support function of keratin IFs in vivo...
  32. pmc Structural basis for heteromeric assembly and perinuclear organization of keratin filaments
    Chang Hun Lee
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA
    Nat Struct Mol Biol 19:707-15. 2012
    ..We show that K14-Cys367 impacts nuclear shape in cultured keratinocytes and that mouse epidermal keratinocytes lacking K14 show aberrations in nuclear structure, highlighting a new function for keratin filaments...
  33. pmc Keratin 16 regulates innate immunity in response to epidermal barrier breach
    Juliane C Lessard
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205
    Proc Natl Acad Sci U S A 110:19537-42. 2013
    ..Our results uncover a role for Krt16 in regulating epithelial inflammation that is relevant to genodermatoses, psoriasis, and cancer and suggest a avenue for the therapeutic management of PC and related disorders. ..
  34. doi request reprint Emerging role for the cytoskeleton as an organizer and regulator of translation
    Seyun Kim
    The Solomon H Snyder Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Nat Rev Mol Cell Biol 11:75-81. 2010
    ....
  35. ncbi request reprint Contribution of olfactory neural stem cells to tissue maintenance and regeneration
    Cheuk T Leung
    Center for Sensory Biology and the Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 N Wolfe Street, PCTB 801, Baltimore, Maryland 21205, USA
    Nat Neurosci 10:720-6. 2007
    ..Our data support a new model of adult neurogenesis in which distinct cell populations mediate normal neuronal turnover and neuronal replacement upon traumatic injury...
  36. pmc Identification of novel interaction between annexin A2 and keratin 17: evidence for reciprocal regulation
    Byung Min Chung
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 287:7573-81. 2012
    ..Down-regulation of K17 expression, in turn, results in decreased AnxA2 phosphorylation at Tyr-23. These findings uncover a novel interaction involving K17 and AnxA2 and identify AnxA2 as a potential regulator of keratin filaments...
  37. pmc Keratin 16-null mice develop palmoplantar keratoderma, a hallmark feature of pachyonychia congenita and related disorders
    Juliane C Lessard
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Invest Dermatol 132:1384-91. 2012
    ..Our findings call into question the view that PC-related PPK arises exclusively as a gain-of-function on account of dominantly acting mutated keratins, and highlight the key role of modifiers in the clinical heterogeneity of PC symptoms...
  38. ncbi request reprint Intermediate filaments and tissue repair
    Daryle DePianto
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Exp Cell Res 301:68-76. 2004
    ..Here we review the literature showing that modulation of cytoplasmic IFs occurs rapidly after injury to a variety of tissues and that such changes play an important role in the context of a timely repair response...
  39. ncbi request reprint Cytoplasmic intermediate filaments revealed as dynamic and multipurpose scaffolds
    Pierre A Coulombe
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Nat Cell Biol 6:699-706. 2004
    ....
  40. ncbi request reprint Keratin 16 expression defines a subset of epithelial cells during skin morphogenesis and the hair cycle
    Kelsie M Bernot
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Invest Dermatol 119:1137-49. 2002
    ....
  41. ncbi request reprint Mouse models of alopecia: identifying structural genes that are baldly needed
    Xuemei Tong
    Dept of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Trends Mol Med 9:79-84. 2003
    ..Here we discuss transgenic mouse models that exhibit alopecia as a primary phenotype, resulting from the inactivation of genes encoding structural proteins...
  42. ncbi request reprint Wound epithelialization: accelerating the pace of discovery
    Pierre A Coulombe
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Invest Dermatol 121:219-30. 2003
  43. pmc Modeling the self-organization property of keratin intermediate filaments
    Jin Seob Kim
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA
    Biophys J 99:2748-56. 2010
    ....
  44. pmc The incidental pore: CaV1.2 and stem cell activation in quiescent hair follicles
    Pierre A Coulombe
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Genes Dev 27:1315-7. 2013
    ..2, the pore-forming subunit in a well-characterized voltage-gated, L-type calcium channel, is expressed in hair follicle stem cells and contributes to anagen re-entry but does so in a calcium flux-independent fashion. ..
  45. pmc Keratin 17 null mice exhibit age- and strain-dependent alopecia
    Kevin M McGowan
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Genes Dev 16:1412-22. 2002
    ....
  46. ncbi request reprint The mechanical properties of simple epithelial keratins 8 and 18: discriminating between interfacial and bulk elasticities
    Soichiro Yamada
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    J Struct Biol 143:45-55. 2003
    ..The potential for modulation of mechanical properties through self-organization may be a general property of keratin polymers and contribute to their organization and function in vivo...
  47. pmc Keratins and protein synthesis: the plot thickens
    Juliane C Kellner
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 20723, USA
    J Cell Biol 187:157-9. 2009
    ..2009. J. Cell Biol. doi:10.1083/jcb.200906094) expand our understanding of how keratin proteins participate in the regulation of protein synthesis through their analysis of mice lacking the entire type II keratin gene cluster...