Robert Cotter

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Development of protein biomarkers in cerebrospinal fluid for secondary progressive multiple sclerosis using selected reaction monitoring mass spectrometry (SRM-MS)
    Yan Jia
    Department of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA
    Clin Proteomics 9:9. 2012
  2. ncbi request reprint Time-of-flights and traps: from the Histone Code to Mars
    Robert J Cotter
    Middle Atlantic Mass Spectrometry Laboratory, Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Eur J Mass Spectrom (Chichester, Eng) 16:331-40. 2010
  3. ncbi request reprint Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron
    Robert J Cotter
    Middle Atlantic Mass Spectrometry Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States
    J Chromatogr B Analyt Technol Biomed Life Sci 855:2-13. 2007
  4. pmc Oxidative stress and the HIV-infected brain proteome
    Lerna Uzasci
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
    J Neuroimmune Pharmacol 8:1167-80. 2013
  5. ncbi request reprint Cleavage of cystatin C in the cerebrospinal fluid of patients with multiple sclerosis
    David N Irani
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA
    Ann Neurol 59:237-47. 2006
  6. ncbi request reprint Approach for determining protein ubiquitination sites by MALDI-TOF mass spectrometry
    Dongxia Wang
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Anal Chem 77:1458-66. 2005
  7. pmc Impairment of adult hippocampal neural progenitor proliferation by methamphetamine: role for nitrotyrosination
    Arun Venkatesan
    Department of Neurology, Johns Hopkins University School of Medicine, 600 N Wolfe St, Baltimore, MD 21287, USA
    Mol Brain 4:28. 2011
  8. pmc Multiple roles for acetylation in the interaction of p300 HAT with ATF-2
    Balasubramanyam Karanam
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Biochemistry 46:8207-16. 2007
  9. pmc A novel role of protein tyrosine kinase2 in mediating chloride secretion in human airway epithelial cells
    Lihua Liang
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    PLoS ONE 6:e21991. 2011
  10. ncbi request reprint A peptide from heat shock protein 60 is the dominant peptide bound to Qa-1 in the absence of the MHC class Ia leader sequence peptide Qdm
    Adrian Davies
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Immunol 170:5027-33. 2003

Research Grants

Collaborators

Detail Information

Publications29

  1. pmc Development of protein biomarkers in cerebrospinal fluid for secondary progressive multiple sclerosis using selected reaction monitoring mass spectrometry (SRM-MS)
    Yan Jia
    Department of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA
    Clin Proteomics 9:9. 2012
    ..abstract:..
  2. ncbi request reprint Time-of-flights and traps: from the Histone Code to Mars
    Robert J Cotter
    Middle Atlantic Mass Spectrometry Laboratory, Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Eur J Mass Spectrom (Chichester, Eng) 16:331-40. 2010
    ..Like the tandem mass spectrometer, it is also expected to have applicability to environmental and biological analyses and, ultimately, to clinical care...
  3. ncbi request reprint Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron
    Robert J Cotter
    Middle Atlantic Mass Spectrometry Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States
    J Chromatogr B Analyt Technol Biomed Life Sci 855:2-13. 2007
    ..For a number of closely spaced sites on the histone tail regions, MS/MS enables us to then determine both the order and distribution of acetylation...
  4. pmc Oxidative stress and the HIV-infected brain proteome
    Lerna Uzasci
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
    J Neuroimmune Pharmacol 8:1167-80. 2013
    ..This review also provides an overview of related research pertaining to oxidative stress-related HAND using cerebrospinal fluid and human brain tissue. ..
  5. ncbi request reprint Cleavage of cystatin C in the cerebrospinal fluid of patients with multiple sclerosis
    David N Irani
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA
    Ann Neurol 59:237-47. 2006
    ..The diagnosis of multiple sclerosis (MS) can be challenging because of the lack of a specific diagnostic test. Recent advances in proteomics, however, offer new opportunities for biomarker discovery and the study of disease pathogenesis...
  6. ncbi request reprint Approach for determining protein ubiquitination sites by MALDI-TOF mass spectrometry
    Dongxia Wang
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Anal Chem 77:1458-66. 2005
    ....
  7. pmc Impairment of adult hippocampal neural progenitor proliferation by methamphetamine: role for nitrotyrosination
    Arun Venkatesan
    Department of Neurology, Johns Hopkins University School of Medicine, 600 N Wolfe St, Baltimore, MD 21287, USA
    Mol Brain 4:28. 2011
    ....
  8. pmc Multiple roles for acetylation in the interaction of p300 HAT with ATF-2
    Balasubramanyam Karanam
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Biochemistry 46:8207-16. 2007
    ..Taken together, these studies suggest multiple roles for protein acetylation in the regulation of transcription by p300/CBP and ATF-2...
  9. pmc A novel role of protein tyrosine kinase2 in mediating chloride secretion in human airway epithelial cells
    Lihua Liang
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    PLoS ONE 6:e21991. 2011
    ..The identification of this novel role of PYK2 reveals a new signaling pathway in human airway epithelial cells...
  10. ncbi request reprint A peptide from heat shock protein 60 is the dominant peptide bound to Qa-1 in the absence of the MHC class Ia leader sequence peptide Qdm
    Adrian Davies
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Immunol 170:5027-33. 2003
    ....
  11. ncbi request reprint Mass correlated acceleration in a reflectron MALDI TOF mass spectrometer: an approach for enhanced resolution over a broad mass range
    Slava V Kovtoun
    Middle Atlantic Mass Spectrometry Laboratory, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Am Soc Mass Spectrom 13:135-43. 2002
    ..We demonstrate the validity of this novel technique with applications in peptide mixture analysis and protein digests of lysozyme and bovine serum albumin...
  12. ncbi request reprint The sirtuins hst3 and Hst4p preserve genome integrity by controlling histone h3 lysine 56 deacetylation
    Ivana Celic
    High Throughput Biology Center, Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Curr Biol 16:1280-9. 2006
    ..Acetylation of histone H3 lysine 56 (K56Ac) occurs transiently in newly synthesized H3 during passage through S phase and is removed in G2. However, the physiologic roles and effectors of K56Ac turnover are unknown...
  13. ncbi request reprint Identification of protein ubiquitylation by electrospray ionization tandem mass spectrometric analysis of sulfonated tryptic peptides
    Dongxia Wang
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Anal Chem 78:3681-7. 2006
    ..The method is demonstrated by the analysis of three ubiquitin-conjugated proteins using LC/MS/MS...
  14. ncbi request reprint Fibroblast activation protein peptide substrates identified from human collagen I derived gelatin cleavage sites
    Saurabh Aggarwal
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University, Baltimore, Maryland 21231, USA
    Biochemistry 47:1076-86. 2008
    ..On the basis of these results, the FAP consensus sequences are currently being evaluated as FAP-selective peptide carriers for incorporation into FAP-activated prodrugs...
  15. ncbi request reprint Direct identification of ubiquitination sites on ubiquitin-conjugated CHIP using MALDI mass spectrometry
    Dongxia Wang
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Proteome Res 4:1554-60. 2005
    ....
  16. ncbi request reprint Structural analysis of a highly acetylated protein using a curved-field reflectron mass spectrometer
    Dongxia Wang
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proteomics 5:2288-96. 2005
    ..Using MS and MS/MS, we were able to characterize both the unmodified and acetylated tryptic peptides covering more than 82% of the protein...
  17. pmc A reductionist cell-free major histocompatibility complex class II antigen processing system identifies immunodominant epitopes
    Isamu Z Hartman
    Graduate Program in Immunology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Med 16:1333-40. 2010
    ..Thus, we provide a new tool for the identification of physiologically relevant helper T cell epitopes from antigens...
  18. pmc Side chain specificity of ADP-ribosylation by a sirtuin
    Kamau Fahie
    Department of Biophysics and Biophysical Chemistry, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    FEBS J 276:7159-76. 2009
    ..These results suggest that TbSIR2RP1 can perform ADP-ribosylation using two distinct mechanisms, depending on whether or not the substrate is acetylated...
  19. doi request reprint Protein arrays on patterned porous gold substrates interrogated with mass spectrometry: detection of peptides in plasma
    Kenyon M Evans-Nguyen
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Anal Chem 80:1448-58. 2008
    ....
  20. ncbi request reprint Fragmentation of phosphopeptides by atmospheric pressure MALDI and ESI/Ion trap mass spectrometry
    Susanne C Moyer
    Department of Chemistry, Johns Hopkins University, Baltimore, Maryland, USA
    J Am Soc Mass Spectrom 13:274-83. 2002
    ....
  21. ncbi request reprint Atmospheric pressure MALDI
    Susanne C Moyer
    Department of Chemistry, John Hopkins University and John Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Anal Chem 74:468A-476A. 2002
  22. ncbi request reprint A miniaturized matrix-assisted laser desorption/ionization time of flight mass spectrometer with mass-correlated acceleration focusing
    Robert D English
    Middle Atlantic Mass Spectrometry Laboratory, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University Schoolof Medicine, Baltimore, Maryland 21205 USA
    J Mass Spectrom 38:296-304. 2003
    ..The technique was validated with applications in peptide mixtures and protein digestions. Diagnostic studies for the instrument include m/z range and limits of detection...
  23. ncbi request reprint Fragmentation of cationized phosphotyrosine containing peptides by atmospheric pressure MALDI/Ion trap mass spectrometry
    Susanne C Moyer
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Am Soc Mass Spectrom 14:581-92. 2003
    ..However, sodium cationized phosphotyrosine containing peptides that do not contain arginine or lysine residues within their sequences do undergo losses of HPO(3) (Delta 80 Da) and HPO(3) + H(2)O (Delta 98 Da) in their CID spectra...
  24. ncbi request reprint Disposable hydrophobic surface on MALDI targets for enhancing MS and MS/MS data of peptides
    Rebekah L Gundry
    Department of Pharmacology and Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Anal Chem 77:6609-17. 2005
    ..An example compound with a complete methodology is shown to increase sample homogeneity, peak intensity, and resolution when used for peptide mixtures with CHCA and DHB...
  25. ncbi request reprint Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis
    Stacey L Wooden
    Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
    J Immunol 175:1383-7. 2005
    ..This report is the first to identify a non-MHC leader inhibitory peptide bound to HLA-E and provides insight into the immunoregulatory role of HLA-E during cell stress...
  26. ncbi request reprint Study of peptide-sugar non-covalent complexes by infrared atmospheric pressure matrix-assisted laser desorption/ionization
    Christopher E Von Seggern
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    J Mass Spectrom 39:736-42. 2004
    ..This technique can be used to study other protein-sugar interactions and can be expanded to create high-throughput screening techniques...
  27. ncbi request reprint Regulation of the p300 HAT domain via a novel activation loop
    Paul R Thompson
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Struct Mol Biol 11:308-15. 2004
    ..We therefore propose that this autoregulatory loop could influence the impact of p300 on a wide variety of signaling and transcriptional events...
  28. ncbi request reprint Improved procedures for N-terminal sulfonation of peptides for matrix-assisted laser desorption/ionization post-source decay peptide sequencing
    Dongxia Wang
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Rapid Commun Mass Spectrom 18:96-102. 2004
    ..The results indicate that the improved sulfonation reaction can be implemented with high efficiency under aqueous conditions and that the sensitivity of mass detection can be increased considerably...
  29. pmc An acetylation site in the middle domain of Hsp90 regulates chaperone function
    Bradley T Scroggins
    Urologic Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Cell 25:151-9. 2007
    ..These data suggest that acetylation/deacetylation of K294 plays an important role in regulating the Hsp90 chaperone cycle...

Research Grants5

  1. TANDEM TIME-OF-FLIGHT FOR PROTEOMICS ANALYSIS
    Robert Cotter; Fiscal Year: 2002
    ..This technology is expected to bring high performance and tandem capabilities to a simple, compact instrument that can be utilized in proteomics research. ..
  2. TANDEM TIME-OF-FLIGHT FOR PROTEOMICS ANALYSIS
    Robert Cotter; Fiscal Year: 2003
    ..This technology is expected to bring high performance and tandem capabilities to a simple, compact instrument that can be utilized in proteomics research. ..
  3. TANDEM TIME-OF-FLIGHT FOR PROTEOMICS ANALYSIS
    Robert Cotter; Fiscal Year: 2004
    ..This technology is expected to bring high performance and tandem capabilities to a simple, compact instrument that can be utilized in proteomics research. ..
  4. TANDEM TIME-OF-FLIGHT FOR PROTEOMICS ANALYSIS
    Robert Cotter; Fiscal Year: 2005
    ..This technology is expected to bring high performance and tandem capabilities to a simple, compact instrument that can be utilized in proteomics research. ..
  5. Purchase of a Linear Ion Trap/Fourier Transform Mass Spectrometer
    Robert Cotter; Fiscal Year: 2007
    ..The instrument will be housed in and managed by the Middle Atlantic Mass Spectrometry Laboratory and operated by four individuals representing the Major Users and collaborators. ..