Linzhao Cheng

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer
    Betty Ying Zhou
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, MD 21205, USA
    Stem Cells 25:779-89. 2007
  2. doi request reprint More new lines of human parthenogenetic embryonic stem cells
    Linzhao Cheng
    Stem Cell Program, Johns Hopkins Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Res 18:215-7. 2008
  3. doi request reprint Lentiviral gene transduction of mouse and human stem cells
    Zhaohui Ye
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD
    Methods Mol Biol 430:243-53. 2008
  4. doi request reprint Stem cells shine in Shanghai
    Linzhao Cheng
    Stem Cell Program, Johns Hopkins Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:34-7. 2008
  5. pmc Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins
    Guibin Chen
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:345-55. 2008
  6. pmc Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures
    Bin Kuan Chou
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cell Res 21:518-29. 2011
  7. pmc Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expression
    Linzhao Cheng
    Stem Cell Program in Institute for Cell Engineering and Division of Hematology, Johns Hopkins University, Baltimore, MD 21205, USA
    Cell Stem Cell 10:337-44. 2012
  8. ncbi request reprint Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture
    Linzhao Cheng
    The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Stem Cells 21:131-42. 2003
  9. ncbi request reprint Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells
    Xiaobing Yu
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Department of Oncology, Baltimore, Maryland 21231, USA
    Mol Ther 7:827-38. 2003
  10. ncbi request reprint HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding
    Xiaobing Yu
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Department of Oncology, Bunting Blaustein Cancer Research Building, Room 2M44, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Stem Cells 24:876-88. 2006

Collaborators

Detail Information

Publications59

  1. ncbi request reprint Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer
    Betty Ying Zhou
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, MD 21205, USA
    Stem Cells 25:779-89. 2007
    ..The lentiviral vectors containing the tTS-suppressive, Dox-inducible system offer a universal, inducible, and reversible transgene expression system in essentially any mammalian cell types, including human embryonic stem cells...
  2. doi request reprint More new lines of human parthenogenetic embryonic stem cells
    Linzhao Cheng
    Stem Cell Program, Johns Hopkins Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Res 18:215-7. 2008
  3. doi request reprint Lentiviral gene transduction of mouse and human stem cells
    Zhaohui Ye
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD
    Methods Mol Biol 430:243-53. 2008
    ..These cells maintained stable transgene expression after engraftment in mice and in vivo differentiation. Human ESCs can also be transduced with a high efficiency, and transgene is expressed stably after hematopoietic differentiation...
  4. doi request reprint Stem cells shine in Shanghai
    Linzhao Cheng
    Stem Cell Program, Johns Hopkins Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:34-7. 2008
    ..Here we present a sampling of the diverse research presented by the local and international participants during the science-packed 4 day meeting...
  5. pmc Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins
    Guibin Chen
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:345-55. 2008
    ..These data reveal that GPI-AP-enhanced full activation of BMP signaling is required for human trophoblast formation...
  6. pmc Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures
    Bin Kuan Chou
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cell Res 21:518-29. 2011
    ..This facile method of generating integration-free human iPSCs from blood MNCs will accelerate their use in both research and future clinical applications...
  7. pmc Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expression
    Linzhao Cheng
    Stem Cell Program in Institute for Cell Engineering and Division of Hematology, Johns Hopkins University, Baltimore, MD 21205, USA
    Cell Stem Cell 10:337-44. 2012
    ..Our data thus suggest that episome-mediated reprogramming is not inherently mutagenic during integration-free iPSC induction...
  8. ncbi request reprint Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture
    Linzhao Cheng
    The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Stem Cells 21:131-42. 2003
    ..In addition, this system may help to identify cytokines and adhesion molecules that are required for the self-renewal of hES cells...
  9. ncbi request reprint Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells
    Xiaobing Yu
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Department of Oncology, Baltimore, Maryland 21231, USA
    Mol Ther 7:827-38. 2003
    ..Thus, such dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell and will enable many gene transfer applications...
  10. ncbi request reprint HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding
    Xiaobing Yu
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Department of Oncology, Bunting Blaustein Cancer Research Building, Room 2M44, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Stem Cells 24:876-88. 2006
    ..Our data characterize the complex, cell context-dependent actions of HES1 as a major downstream Notch signaling regulator of HSPC function...
  11. ncbi request reprint Transduction of donor hematopoietic stem-progenitor cells with Fas ligand enhanced short-term engraftment in a murine model of allogeneic bone marrow transplantation
    Katharine A Whartenby
    Sidney Kimmel Comprehensive Cancer Center at JHU, School of Medicine, Johns Hopkins University, Bunting Blaustein Cancer Research Building, Room 2M44, 1650 Orleans Street, Baltimore, MD 21231, USA
    Blood 100:3147-54. 2002
    ..These results suggest potential therapeutic approaches by manipulating lymphohematopoietic stem-progenitor cells to express FasL or other immune-modulating genes in the context of BMT...
  12. ncbi request reprint Immunotherapy of established tumors using bone marrow transplantation with antigen gene--modified hematopoietic stem cells
    Yan Cui
    Immunology and Hematopoiesis Division, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Nat Med 9:952-8. 2003
    ..This tripartite strategy provided potent antigen-specific immunotherapy for an aggressive established tumor...
  13. ncbi request reprint Lentivirus-mediated gene transfer and expression in established human tumor antigen-specific cytotoxic T cells and primary unstimulated T cells
    Xianzheng Zhou
    Division of Immunology and Hematppoiesis, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Hum Gene Ther 14:1089-105. 2003
    ..These results will have significant implications for the study of T-cell biology and for the improvement of clinical gene therapies of acquired immune deficiency syndrome (AIDS) and cancer...
  14. ncbi request reprint Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 North Broadway, Baltimore, Maryland 21205, USA
    Stem Cells 26:1998-2005. 2008
    ..Using this improved approach, we also generated hES-like cells from homozygous fibroblasts containing the sickle cell anemia mutation Hemoglobin Sickle. Disclosure of potential conflicts of interest is found at the end of this article...
  15. ncbi request reprint FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cells
    Li Li
    Department of Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA
    Br J Haematol 137:64-75. 2007
    ..CEP-701 may act as a potent therapeutic agent for AML stem cells harbouring FLT3/ITD mutations...
  16. pmc Concise review: stem cell-based approaches to red blood cell production for transfusion
    Siddharth Shah
    Division of Hematology, Department of Medicine, and Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Stem Cells Transl Med 3:346-55. 2014
    ..In this review, we focus on current research and technologies that permit RBC production ex vivo from hematopoietic stem cells, pluripotent stem cells, and immortalized erythroid precursors. ..
  17. pmc Notch signaling activation in human embryonic stem cells is required for embryonic, but not trophoblastic, lineage commitment
    Xiaobing Yu
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 2:461-71. 2008
    ..These new insights into the roles of Notch in hESC-fate determination may help to efficiently direct hESC differentiation into therapeutically relevant cell types...
  18. ncbi request reprint Scalable expansion of human induced pluripotent stem cells in the defined xeno-free E8 medium under adherent and suspension culture conditions
    Ying Wang
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA Stem Cell Program, Institute of Cell Engineering, The Johns Hopkins University School of Medicine, Edward D Miller Research Building, Room 747, 733 N Broadway, Baltimore, MD 21205, USA Institute for NanoBioTechnology, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    Stem Cell Res 11:1103-16. 2013
    ..This system is the first to enable an efficient scale-up bioprocess in completely xeno-free condition for the expansion and cryopreservation of hiPSCs with the quantity and quality compliant for clinical applications. ..
  19. pmc Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders
    Zhaohui Ye
    Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Blood 114:5473-80. 2009
    ..These iPS cells provide a renewable cell source and a prospective hematopoiesis model for investigating MPD pathogenesis...
  20. ncbi request reprint Culture of human embryonic stem cells on human and mouse feeder cells
    Gautam Dravid
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 331:91-104. 2006
    ....
  21. ncbi request reprint Serial imaging of human embryonic stem-cell engraftment and teratoma formation in live mouse models
    Martin G Pomper
    Department of Radiology and Radiological Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA
    Cell Res 19:370-9. 2009
    ..Noninvasive imaging methods such as these may enable us to monitor the presence and distribution of transplanted human stem cells repetitively within live recipients over a long term through the expression of a reporter gene...
  22. ncbi request reprint Functional antigen-presenting leucocytes derived from human embryonic stem cells in vitro
    Xiangcan Zhan
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Lancet 364:163-71. 2004
    ..We aimed to develop methods to efficiently differentiate hES cells to haemopoietic cells, including immune-modulating leucocytes, a prerequisite of the tolerance induction strategies applying to hES cell-mediated transplantation...
  23. pmc Oxidase-deficient neutrophils from X-linked chronic granulomatous disease iPS cells: functional correction by zinc finger nuclease-mediated safe harbor targeting
    Jizhong Zou
    Division of Hematology, Department of Medicine, and Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Blood 117:5561-72. 2011
    ..Our findings demonstrate how precise gene targeting may be applied to correction of X-CGD using zinc finger nuclease and patient iPSCs...
  24. pmc In vivo functional efficacy of tumor-specific T cells expanded using HLA-Ig based artificial antigen presenting cells (aAPC)
    Malarvizhi Durai
    Department of Pathology, Johns Hopkins School of Medicine, Ross 664G, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Cancer Immunol Immunother 58:209-20. 2009
    ..Thus, our data demonstrate potential therapeutic in vivo activity of HLA-Ig based aAPC expanded CTL to control tumor growth...
  25. ncbi request reprint Radiofrequency-enhanced vascular gene transduction and expression for intravascular MR imaging-guided therapy: feasibility study in pigs
    Xiangying Du
    Department of Radiology, Johns Hopkins University School of Medicine, Traylor Bldg, Room 330, 720 Rutland Ave, Baltimore, MD 21205, USA
    Radiology 236:939-44. 2005
    ..To evaluate the feasibility of radiofrequency (RF)-enhanced vascular gene transduction and expression by using a magnetic resonance (MR) imaging-heating guidewire as an intravascular heating vehicle during MR imaging-guided therapy...
  26. ncbi request reprint Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions
    Liuhong Cai
    Institute for Cell Engineering, Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Res 17:62-72. 2007
    ....
  27. pmc Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression
    Sarah N Dowey
    Stem Cell Program, Institute for Cell Engineering, Division of Hematology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Protoc 7:2013-21. 2012
    ..We describe here a detailed, validated protocol for effective generation of integration-free human iPSCs from blood MNCs by plasmid vectors...
  28. pmc Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells
    Jizhong Zou
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 5:97-110. 2009
    ..Our study provides the first demonstration of HR-mediated gene targeting in hiPSCs and shows the power of ZFNs for inducing specific genetic modifications in hiPSCs, as well as hESCs...
  29. pmc Butyrate greatly enhances derivation of human induced pluripotent stem cells by promoting epigenetic remodeling and the expression of pluripotency-associated genes
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Stem Cells 28:713-20. 2010
    ..Moreover, butyrate stimulation provides an efficient method for reprogramming various human adult somatic cells, including cells from patients that are more refractory to reprogramming...
  30. doi request reprint An improved method for generating and identifying human induced pluripotent stem cells
    Prashant Mali
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 636:191-205. 2010
    ..Finally, we elaborate a robust technique for monitoring and identification of potential iPS cells through live staining of reprogrammed cells. We also outline steps for characterization of the resulting iPS cell lines...
  31. pmc Site-specific gene correction of a point mutation in human iPS cells derived from an adult patient with sickle cell disease
    Jizhong Zou
    Institute for Cell Engineering, The JohnsHopkins University School of Medicine, Baltimore, MD, USA
    Blood 118:4599-608. 2011
    ..This study also provides a new strategy for gene therapy of monogenic diseases using patient-specific iPSCs, even if the underlying disease-causing mutation is not expressed in iPSCs...
  32. ncbi request reprint How reproducible is bioluminescent imaging of tumor cell growth? Single time point versus the dynamic measurement approach
    Shingo Baba
    Division of Nuclear Medicine, The Russell H Morgan Department of Radiology and Radiological Science, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Mol Imaging 6:315-22. 2007
    ..This simplified single time point measurement appears appropriate to estimate the total tumor burden in this model, but the substantial variance at each measurement must be considered in experimental designs...
  33. ncbi request reprint Antitumor activity of the histone deacetylase inhibitor MS-275 in prostate cancer models
    David Z Qian
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
    Prostate 67:1182-93. 2007
    ..In this study, we tested the biological effects of the HDAC inhibitor MS-275 in various pre-clinical prostate cancer models both in'vitro and in vivo...
  34. ncbi request reprint Detection of dual-gene expression in arteries using an optical imaging method
    Hunter H Chen
    Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205, USA
    J Biomed Opt 9:1223-9. 2004
    ..We demonstrate the use of an optical imaging method to concurrently detect two distinct fluorescent proteins, potentially permitting the expression of multiple transgenes and their localization in the vasculature to be monitored...
  35. pmc Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
    Sravanti Kusuma
    Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences Oncology Center and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 110:12601-6. 2013
    ....
  36. pmc Differential sensitivity to JAK inhibitory drugs by isogenic human erythroblasts and hematopoietic progenitors generated from patient-specific induced pluripotent stem cells
    Zhaohui Ye
    Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Stem Cells 32:269-78. 2014
    ..The iPSC-mediated disease modeling thus underlies the ineffectiveness of the current JAK inhibitors and provides a modeling system to develop better targeted therapies for the JAK2 mutated hematopoiesis...
  37. pmc Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells
    Joyce E Ohm
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Res 70:7662-73. 2010
    ..Our findings suggest that by studying the process of induced reprogramming, we may gain significant insight into the origins of epigenetic gene silencing associated with human tumorigenesis, and add to means of assessing iPS for safety...
  38. pmc The differential formation of the LINC-mediated perinuclear actin cap in pluripotent and somatic cells
    Shyam B Khatau
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 7:e36689. 2012
    ..While, the localization of lamin A/C at the nuclear envelope is required for perinuclear actin cap formation, it is not sufficient to control nuclear shape...
  39. doi request reprint Concise review: Human cell engineering: cellular reprogramming and genome editing
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Stem Cells 30:75-81. 2012
    ..The objective of this review is to summarize the key enabling developments on these two rapidly evolving research fronts in human cell engineering, highlight unresolved issues, and outline potential future research directions...
  40. pmc Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent stem cells
    Su Mi Choi
    Stem Cell Biology Laboratory, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Blood 118:1801-5. 2011
    ..The ability to reprogram the widely banked patient B-cell lines will offer an unprecedented opportunity to generate human disease models and provide novel drug therapies...
  41. ncbi request reprint Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells
    Gautam Dravid
    The Institute for Cell Engineering, Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Stem Cells 23:1489-501. 2005
    ..Thus, Wnt/beta-catenin activation does not suffice to maintain the undifferentiated and pluripotent state of hESCs. We propose a new model for the role of Wnt/beta-catenin signaling in undifferentiated hESCs...
  42. pmc The high-mobility group A1a/signal transducer and activator of transcription-3 axis: an achilles heel for hematopoietic malignancies?
    Joelle Hillion
    Departments of Medicine, Hematology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Cancer Res 68:10121-7. 2008
    ..Our results show that the HMGA1a-STAT3 axis is a potential Achilles heel that could be exploited therapeutically in hematopoietic and other malignancies overexpressing HMGA1a...
  43. pmc Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing
    Wei Yan
    Stem Cell Program, Institute for Cell Engineering, Baltimore, MD 21205, USA
    Sci Rep 3:2376. 2013
    ....
  44. ncbi request reprint And then there were none: no need for pluripotency factors to induce reprogramming
    Bin Kuan Chou
    Stem Cell Program in the Institute for Cell Engineering and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell Stem Cell 13:261-2. 2013
    ..Three recent papers have now broken this barrier through the use of opposing lineage specifying transgenes and chemical modulation, thus signifying a milestone in advancing our understanding of pluripotency induction. ..
  45. pmc Glycosylphosphatidylinositol-anchored protein deficiency confers resistance to apoptosis in PNH
    William J Savage
    Division of Hematology, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2196, USA
    Exp Hematol 37:42-51. 2009
    ..Investigate the contribution of PIG-A mutations to clonal expansion in paroxysmal nocturnal hemoglobinuria (PNH)...
  46. ncbi request reprint Molecular imaging and stem cell research
    Yoon Young Jang
    Sidney Kimmel Comprehensive Cancer Center, JohnsHopkins University School of Medicine, Baltimore, MD 21231, USA
    Mol Imaging 10:111-22. 2011
    ..In this review, we discuss the application of current imaging modalities for research of hematopoietic stem cells and pluripotent stem cells and the challenges ahead...
  47. pmc In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells
    Nathaniel S Hwang
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 105:20641-6. 2008
    ..In view of the limited available cell sources for tissue engineering applications, these embryonic-derived cells show significant potential in musculoskeletal tissue regeneration applications...
  48. pmc Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors
    Xiaosong Huang
    1 Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 2 Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Mol Ther 22:451-63. 2014
    ..Our result may ultimately lead to an alternative approach to generate unlimited numbers of RBCs for personalized transfusion medicine. ..
  49. doi request reprint Promise and challenges of human iPSC-based hematologic disease modeling and treatment
    Zhaohui Ye
    Division of Hematology in Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Research Building, Room 1032, Baltimore, MD 21205, USA
    Int J Hematol 95:601-9. 2012
    ..Here, we review the recent development in iPSC-based blood disease modeling, and discuss the unsolved issues and challenges in this new and promising field...
  50. ncbi request reprint Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells
    Yan Cui
    Division of Immunology and Hematopoiesis, Johns Hopkins Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA
    Blood 99:399-408. 2002
    ..This study demonstrated successful targeting of transgene expression to APCs/DCs after stable gene transduction of pluripotent HSCs...
  51. ncbi request reprint Myocyte enhancer factor 2 mediates calcium-dependent transcription of the interleukin-2 gene in T lymphocytes: a calcium signaling module that is distinct from but collaborates with the nuclear factor of activated T cells (NFAT)
    Fan Pan
    Department of Pharmacology and Molecular Science and Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:14477-80. 2004
    ..These results suggest that MEF2 is required for the transcriptional activation of IL-2 and likely other cytokine genes in response to calcium signaling and may serve as a novel target for development of immunosuppressants...
  52. ncbi request reprint Efficient and scalable expansion of human pluripotent stem cells under clinically compliant settings: a view in 2013
    Ying Wang
    Department of Chemical and Biomolecular Engineering and Institute for NanoBioTechnology, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA
    Ann Biomed Eng 42:1357-72. 2014
    ....
  53. pmc Potential of human induced pluripotent stem cells derived from blood and other postnatal cell types
    Zhaohui Ye
    Division of Hematology and Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Broadway Research Building, Room 747, 733 N Broadway, Baltimore, MD 21205, USA
    Regen Med 5:521-30. 2010
    ..Advantages of blood as a source for reprogramming and applications in regenerative medicine will be discussed...
  54. pmc HIF-dependent antitumorigenic effect of antioxidants in vivo
    Ping Gao
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cancer Cell 12:230-8. 2007
    ..These findings challenge the paradigm that antioxidants diminish tumorigenesis primarily through decreasing DNA damage and mutations and provide significant support for a key antitumorigenic effect of diminishing HIF levels...
  55. ncbi request reprint PC3, but not DU145, human prostate cancer cells retain the coregulators required for tumor suppressor ability of androgen receptor
    Ivan V Litvinov
    Chemical Therapeutics Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Prostate 66:1329-38. 2006
    ..While these cells do not express AR, it is unclear whether they retained the coactivators necessary for AR-dependent tumor suppression. To answer this question the response to AR protein expression by PC3 and DU145 cells was evaluated...
  56. ncbi request reprint The dazzle in germ cell differentiation
    Candace L Kerr
    Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Mol Cell Biol 2:26-9. 2010
    ..Although factors that Dazl influences during this process have been identified, the underlying mechanisms warrant future studies...
  57. ncbi request reprint TEL-AML1, expressed from t(12;21) in human acute lymphocytic leukemia, induces acute leukemia in mice
    Florence Bernardin
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Cancer Res 62:3904-8. 2002
    ..Overall, these findings indicate that TEL-AML1 contributes to leukemogenesis and may cooperate with loss of p16(INK4a)p14(ARF) to transform lymphoid progenitors...
  58. pmc Dissection of the c-Kit signaling pathway in mouse primordial germ cells by retroviral-mediated gene transfer
    Maria P De Miguel
    Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, Room 706, 233 South 10th Street, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 99:10458-63. 2002
    ..Such technology for manipulating gene expression in PGCs will allow many of the molecular mechanisms regulating germ cell growth, behavior, and differentiation to be comprehensively analyzed...
  59. ncbi request reprint Cotransplantation of human mesenchymal stem cells enhances human myelopoiesis and megakaryocytopoiesis in NOD/SCID mice
    Maria Angelopoulou
    Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520 8035, USA
    Exp Hematol 31:413-20. 2003
    ..Because cotransplantation of marrow-derived stromal cells has been shown to enhance engraftment of human hematopoietic stem cells, we hypothesized that cotransplantation of MSC could enhance platelet and myeloid cell development...

Research Grants8

  1. Directing human ES and EBD cells to blood/immune cells
    Linzhao Cheng; Fiscal Year: 2003
    ..It will also provide a foundation for developing novel ES cell-based therapies that require reconstituting or re-programming patient's blood/immune systems. ..
  2. Directing human ES and EBD cells to blood/immune cells.
    Linzhao Cheng; Fiscal Year: 2004
    ..It will also provide a foundation for developing novel ES cell-based therapies that require reconstituting or re-programming patient's blood/immune systems. ..
  3. Directing human ES and EBD cells to blood/immune cells.
    Linzhao Cheng; Fiscal Year: 2005
    ..It will also provide a foundation for developing novel ES cell-based therapies that require reconstituting or re-programming patient's blood/immune systems. ..
  4. Directing human ES and EBD cells to blood/immune cells.
    Linzhao Cheng; Fiscal Year: 2006
    ..It will also provide a foundation for developing novel ES cell-based therapies that require reconstituting or re-programming patient's blood/immune systems. ..
  5. Human embryonic stem and embroyonic germ cells to blood and immune cells
    Linzhao Cheng; Fiscal Year: 2007
    ..It will also provide a foundation for developing novel ES cell-based therapies that require reconstituting or re-programming patient's blood/immune systems. ..
  6. Directed hematopoietic differentiation of human pluripotent stem cells
    Linzhao Cheng; Fiscal Year: 2010
    ..This study will also help us to establish a universal method to study dozens of other somatic blood diseases as well as inherited blood diseases such as sickle cell anemia. ..