Research Topics
| Vern CarruthersSummaryAffiliation: Johns Hopkins Bloomberg School of Public Health Country: USA Publications
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Detail Information
Publications
Ethanol and acetaldehyde elevate intracellular [Ca2+] and stimulate microneme discharge in Toxoplasma gondiiV B Carruthers
Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
Biochem J 342:379-86. 1999..Thus, because it is genetically tractable, T. gondii might be a convenient model organism for studying the Ca(2+)-elevating effects of alcohol in higher eukaryotes...- Proteolysis and Toxoplasma invasionVern B Carruthers
W Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Room E5136, Baltimore, MD 21205, USA
Int J Parasitol 36:595-600. 2006..The identification of highly selective inhibitors for these proteases has the potential to not only further dissect their roles in infection but also to ameliorate disease... - Pulling together: an integrated model of Toxoplasma cell invasionVern Carruthers
W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205 1918, USA
Curr Opin Microbiol 10:83-9. 2007..These recent findings have led to new hypotheses regarding the parasite's broad host-specificity... - A new release on life: emerging concepts in proteolysis and parasite invasionVern B Carruthers
W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
Mol Microbiol 55:1617-30. 2005..The sequential proteolytic events associated with invasion by pathogenic protozoa may represent vulnerable pathways for the future development of synergistic anti-protozoal therapies... - Host cell invasion by the opportunistic pathogen Toxoplasma gondiiVern B Carruthers
W Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins University Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
Acta Trop 81:111-22. 2002..gondii... - The Toxoplasma adhesive protein MIC2 is proteolytically processed at multiple sites by two parasite-derived proteasesV B Carruthers
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 275:14346-53. 2000..These results establish that MIC2 is proteolytically modified at multiple sites by two distinct enzymes that probably operate on the parasite surface... - Armed and dangerous: Toxoplasma gondii uses an arsenal of secretory proteins to infect host cellsV B Carruthers
Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205, USA
Parasitol Int 48:1-10. 1999..However, as more T. gondii secretory proteins are identified and characterized, it is likely that additional functions will be ascribed to each class of proteins secreted- by this fascinating invasive parasite... - Secretion of micronemal proteins is associated with toxoplasma invasion of host cellsV B Carruthers
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
Cell Microbiol 1:225-35. 1999..Collectively, these observations strongly imply that micronemal proteins play a role in Toxoplasma invasion of host cells... - Toxoplasma MIC2 is a major determinant of invasion and virulenceMy-Hang Huynh
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
PLoS Pathog 2:e84. 2006..Our findings demonstrate that the MIC2 protein complex is a major virulence determinant for Toxoplasma infection and that MIC2-deficient parasites constitute an effective live-attenuated vaccine for experimental toxoplasmosis... - Preparing for an invasion: charting the pathway of adhesion proteins to Toxoplasma micronemesMy-Hang Huynh
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
Parasitol Res 98:389-95. 2006..gondii and at a deeper understanding of the precise roles of M2AP propeptide and MIC2 targeting motifs in MIC protein trafficking... - Proteomic analysis of cleavage events reveals a dynamic two-step mechanism for proteolysis of a key parasite adhesive complexXing W Zhou
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
Mol Cell Proteomics 3:565-76. 2004..Our data support a novel two-step proteolysis model that includes primary processing of the MIC2/M2AP complex followed by secondary cleavage to shed the complex from the parasite surface during the final steps of invasion... - Cysteine protease inhibitors block Toxoplasma gondii microneme secretion and cell invasionChin Fen Teo
Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health, Baltimore, MD 21205, USA
Antimicrob Agents Chemother 51:679-88. 2007..Our findings suggest that cysteine protease activity is required for microneme secretion and cell invasion by T. gondii... - A cleavable propeptide influences Toxoplasma infection by facilitating the trafficking and secretion of the TgMIC2-M2AP invasion complexJill M Harper
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
Mol Biol Cell 17:4551-63. 2006..Therefore, the propeptide and its cleavage site influence distinct aspects of TgMIC2-M2AP function, with both impacting the outcome of infection... - The opportunistic pathogen Toxoplasma gondii deploys a diverse legion of invasion and survival proteinsXing W Zhou
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
J Biol Chem 280:34233-44. 2005..gondii tachyzoites. These findings provided a uniquely broad view of Toxoplasma secretory proteins that participate in parasite survival and pathogenesis during infection... - Two separate, conserved acidic amino acid domains within the Toxoplasma gondii MIC2 cytoplasmic tail are required for parasite survivalG Lucas Starnes
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63130-1093, USA
J Biol Chem 281:30745-54. 2006..Both acidic domains are conserved throughout TRAP orthologs, implicating a central role for these motifs in apicomplexan motility... - Rapid invasion of host cells by Toxoplasma requires secretion of the MIC2-M2AP adhesive protein complexMy-Hang Huynh
W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
EMBO J 22:2082-90. 2003..M2AP knockout parasites were also unable to rapidly secrete MIC2, an event that normally accompanies parasite attachment to a target cell. These findings indicate a critical role for the MIC2-M2AP protein complex in parasite invasion... - Trans-genera reconstitution and complementation of an adhesion complex in Toxoplasma gondiiMy-Hang Huynh
Johns Hopkins Bloomberg School of Public Health, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Baltimore MD, USA
Cell Microbiol 6:771-82. 2004.... - A transient forward-targeting element for microneme-regulated secretion in Toxoplasma gondiiSusannah D Brydges
W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
Biol Cell 100:253-64. 2008..Microneme discharge during parasite apical attachment plays a pivotal role in cell invasion by delivering adhesive proteins for host receptor engagement... - Multimerization of the Toxoplasma gondii MIC2 integrin-like A-domain is required for binding to heparin and human cellsJill M Harper
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street E5132, Baltimore, MD 21205, USA
Mol Biochem Parasitol 134:201-12. 2004..Multimerization of MIC2 may increase the number of interactions with host cell receptors, thereby forming a multivalent adhesive junction during parasite entry... - Identification and partial characterization of a second Kazal inhibitor in Toxoplasma gondiiMeredith Teilhet Morris
The W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
Mol Biochem Parasitol 128:119-22. 2003 - Is Toxoplasma egress the first step in invasion?Eleanor F Hoff
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe St, Baltimore, MD 21205, USA
Trends Parasitol 18:251-5. 2002.... - Targeted deletion of MIC5 enhances trimming proteolysis of Toxoplasma invasion proteinsSusannah D Brydges
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, MD 21205, USA
Eukaryot Cell 5:2174-83. 2006..Together, our findings suggest that MIC5 either directly regulates MPP2 activity or it influences MPP2's ability to access substrate cleavage sites on the parasite surface... - Functional analysis of Toxoplasma gondii protease inhibitor 1Meredith Teilhet Morris
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
J Biol Chem 277:45259-66. 2002..TgPI-1 is the first anti-trypsin/chymotrypsin inhibitor to be identified in bradyzoites and sporozoites, stages of the parasite that would be exposed to proteolytic enzymes in the digestive tract of the host... - The novel coccidian micronemal protein MIC11 undergoes proteolytic maturation by sequential cleavage to remove an internal propeptideJill M Harper
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA
Int J Parasitol 34:1047-58. 2004..This is the first microneme protein characterised to date in the phylum Apicomplexa that possesses this unique structure and undergoes maturation by removal of an internal propeptide... - Mutation of an unusual mitochondrial targeting sequence of SODB2 produces multiple targeting fates in Toxoplasma gondiiSusannah D Brydges
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA
J Cell Sci 116:4675-85. 2003..In addition to having an unusual targeting sequence, TgSODB2 is only the second mitochondrially imported, iron-containing SOD to be described... - Neospora caninum expresses an unusual single-domain Kazal protease inhibitor that is discharged into the parasitophorous vacuoleMeredith Teilhet Morris
W Harry Feinstein Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
Int J Parasitol 34:693-701. 2004..caninum lysate, one with a molecular mass approximately 11 kDa and another at approximately 20 kDa. This, along with mass spectrometry analysis of recombinant NcPI-S, suggests that the inhibitor is expressed as a dimer in the parasite... - Analysis of the Sarcocystis neurona microneme protein SnMIC10: protein characteristics and expression during intracellular developmentJessica S Hoane
Department of Veterinary Science, University of Kentucky, 108 Gluck Equine Research Center, Lexington, KY 40546 0099, USA
Int J Parasitol 33:671-9. 2003..Identification and characterisation of additional S. neurona microneme antigens and comparisons to orthologues in other Apicomplexa could provide further insight into the functions that these proteins serve during invasion of host cells...