Vern Carruthers

Summary

Affiliation: Johns Hopkins Bloomberg School of Public Health
Country: USA

Publications

  1. pmc Ethanol and acetaldehyde elevate intracellular [Ca2+] and stimulate microneme discharge in Toxoplasma gondii
    V B Carruthers
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    Biochem J 342:379-86. 1999
  2. ncbi request reprint Proteolysis and Toxoplasma invasion
    Vern B Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Room E5136, Baltimore, MD 21205, USA
    Int J Parasitol 36:595-600. 2006
  3. ncbi request reprint Pulling together: an integrated model of Toxoplasma cell invasion
    Vern Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205 1918, USA
    Curr Opin Microbiol 10:83-9. 2007
  4. ncbi request reprint A new release on life: emerging concepts in proteolysis and parasite invasion
    Vern B Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Mol Microbiol 55:1617-30. 2005
  5. ncbi request reprint Host cell invasion by the opportunistic pathogen Toxoplasma gondii
    Vern B Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins University Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Acta Trop 81:111-22. 2002
  6. ncbi request reprint The Toxoplasma adhesive protein MIC2 is proteolytically processed at multiple sites by two parasite-derived proteases
    V B Carruthers
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 275:14346-53. 2000
  7. ncbi request reprint Armed and dangerous: Toxoplasma gondii uses an arsenal of secretory proteins to infect host cells
    V B Carruthers
    Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205, USA
    Parasitol Int 48:1-10. 1999
  8. ncbi request reprint Secretion of micronemal proteins is associated with toxoplasma invasion of host cells
    V B Carruthers
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Microbiol 1:225-35. 1999
  9. pmc Toxoplasma MIC2 is a major determinant of invasion and virulence
    My Hang Huynh
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
    PLoS Pathog 2:e84. 2006
  10. ncbi request reprint Preparing for an invasion: charting the pathway of adhesion proteins to Toxoplasma micronemes
    My Hang Huynh
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Parasitol Res 98:389-95. 2006

Collaborators

  • L D Sibley
  • M J Blackman
  • Isabelle Coppens
  • John C Boothroyd
  • F M Tomley
  • Silvia N Moreno
  • B Striepen
  • Jill M Harper
  • My Hang Huynh
  • Susannah D Brydges
  • Xing W Zhou
  • Meredith Teilhet Morris
  • Eleanor F Hoff
  • Chin Fen Teo
  • Fabiola Parussini
  • Xing Wang Zhou
  • G Lucas Starnes
  • Bjorn F C Kafsack
  • Jessica S Hoane
  • Matthew Bogyo
  • Koku D Z Adjogble
  • Walter Daubener
  • Jeffrey Mital
  • Gary E Ward
  • Travis J Jewett
  • Phil Beckett
  • Robert N Cole
  • Rong F Shen
  • Lai Yu Kwok
  • Steven A Howell
  • Wen Chih Cheng
  • Viviana Pszenny
  • Dominique Soldati
  • Corinna Opitz
  • Daniel K Howe
  • David P Morrison
  • Rolf Entzeroth
  • Wandy L Beatty
  • Karen E Rabenau
  • Alexandra Coppin
  • Stanislas Tomavo

Detail Information

Publications27

  1. pmc Ethanol and acetaldehyde elevate intracellular [Ca2+] and stimulate microneme discharge in Toxoplasma gondii
    V B Carruthers
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    Biochem J 342:379-86. 1999
    ..Thus, because it is genetically tractable, T. gondii might be a convenient model organism for studying the Ca(2+)-elevating effects of alcohol in higher eukaryotes...
  2. ncbi request reprint Proteolysis and Toxoplasma invasion
    Vern B Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Room E5136, Baltimore, MD 21205, USA
    Int J Parasitol 36:595-600. 2006
    ..The identification of highly selective inhibitors for these proteases has the potential to not only further dissect their roles in infection but also to ameliorate disease...
  3. ncbi request reprint Pulling together: an integrated model of Toxoplasma cell invasion
    Vern Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205 1918, USA
    Curr Opin Microbiol 10:83-9. 2007
    ..These recent findings have led to new hypotheses regarding the parasite's broad host-specificity...
  4. ncbi request reprint A new release on life: emerging concepts in proteolysis and parasite invasion
    Vern B Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Mol Microbiol 55:1617-30. 2005
    ..The sequential proteolytic events associated with invasion by pathogenic protozoa may represent vulnerable pathways for the future development of synergistic anti-protozoal therapies...
  5. ncbi request reprint Host cell invasion by the opportunistic pathogen Toxoplasma gondii
    Vern B Carruthers
    W Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins University Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Acta Trop 81:111-22. 2002
    ..gondii...
  6. ncbi request reprint The Toxoplasma adhesive protein MIC2 is proteolytically processed at multiple sites by two parasite-derived proteases
    V B Carruthers
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 275:14346-53. 2000
    ..These results establish that MIC2 is proteolytically modified at multiple sites by two distinct enzymes that probably operate on the parasite surface...
  7. ncbi request reprint Armed and dangerous: Toxoplasma gondii uses an arsenal of secretory proteins to infect host cells
    V B Carruthers
    Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205, USA
    Parasitol Int 48:1-10. 1999
    ..However, as more T. gondii secretory proteins are identified and characterized, it is likely that additional functions will be ascribed to each class of proteins secreted- by this fascinating invasive parasite...
  8. ncbi request reprint Secretion of micronemal proteins is associated with toxoplasma invasion of host cells
    V B Carruthers
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Microbiol 1:225-35. 1999
    ..Collectively, these observations strongly imply that micronemal proteins play a role in Toxoplasma invasion of host cells...
  9. pmc Toxoplasma MIC2 is a major determinant of invasion and virulence
    My Hang Huynh
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
    PLoS Pathog 2:e84. 2006
    ..Our findings demonstrate that the MIC2 protein complex is a major virulence determinant for Toxoplasma infection and that MIC2-deficient parasites constitute an effective live-attenuated vaccine for experimental toxoplasmosis...
  10. ncbi request reprint Preparing for an invasion: charting the pathway of adhesion proteins to Toxoplasma micronemes
    My Hang Huynh
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Parasitol Res 98:389-95. 2006
    ..gondii and at a deeper understanding of the precise roles of M2AP propeptide and MIC2 targeting motifs in MIC protein trafficking...
  11. ncbi request reprint Proteomic analysis of cleavage events reveals a dynamic two-step mechanism for proteolysis of a key parasite adhesive complex
    Xing W Zhou
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Mol Cell Proteomics 3:565-76. 2004
    ..Our data support a novel two-step proteolysis model that includes primary processing of the MIC2/M2AP complex followed by secondary cleavage to shed the complex from the parasite surface during the final steps of invasion...
  12. pmc Cysteine protease inhibitors block Toxoplasma gondii microneme secretion and cell invasion
    Chin Fen Teo
    Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health, Baltimore, MD 21205, USA
    Antimicrob Agents Chemother 51:679-88. 2007
    ..Our findings suggest that cysteine protease activity is required for microneme secretion and cell invasion by T. gondii...
  13. pmc A cleavable propeptide influences Toxoplasma infection by facilitating the trafficking and secretion of the TgMIC2-M2AP invasion complex
    Jill M Harper
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Mol Biol Cell 17:4551-63. 2006
    ..Therefore, the propeptide and its cleavage site influence distinct aspects of TgMIC2-M2AP function, with both impacting the outcome of infection...
  14. pmc The opportunistic pathogen Toxoplasma gondii deploys a diverse legion of invasion and survival proteins
    Xing W Zhou
    The W Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Biol Chem 280:34233-44. 2005
    ..gondii tachyzoites. These findings provided a uniquely broad view of Toxoplasma secretory proteins that participate in parasite survival and pathogenesis during infection...
  15. ncbi request reprint Two separate, conserved acidic amino acid domains within the Toxoplasma gondii MIC2 cytoplasmic tail are required for parasite survival
    G Lucas Starnes
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63130 1093, USA
    J Biol Chem 281:30745-54. 2006
    ..Both acidic domains are conserved throughout TRAP orthologs, implicating a central role for these motifs in apicomplexan motility...
  16. pmc Rapid invasion of host cells by Toxoplasma requires secretion of the MIC2-M2AP adhesive protein complex
    My Hang Huynh
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    EMBO J 22:2082-90. 2003
    ..M2AP knockout parasites were also unable to rapidly secrete MIC2, an event that normally accompanies parasite attachment to a target cell. These findings indicate a critical role for the MIC2-M2AP protein complex in parasite invasion...
  17. ncbi request reprint Trans-genera reconstitution and complementation of an adhesion complex in Toxoplasma gondii
    My Hang Huynh
    Johns Hopkins Bloomberg School of Public Health, W Harry Feinstone Department of Molecular Microbiology and Immunology, Baltimore MD, USA
    Cell Microbiol 6:771-82. 2004
    ....
  18. pmc A transient forward-targeting element for microneme-regulated secretion in Toxoplasma gondii
    Susannah D Brydges
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Biol Cell 100:253-64. 2008
    ..Microneme discharge during parasite apical attachment plays a pivotal role in cell invasion by delivering adhesive proteins for host receptor engagement...
  19. ncbi request reprint Multimerization of the Toxoplasma gondii MIC2 integrin-like A-domain is required for binding to heparin and human cells
    Jill M Harper
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street E5132, Baltimore, MD 21205, USA
    Mol Biochem Parasitol 134:201-12. 2004
    ..Multimerization of MIC2 may increase the number of interactions with host cell receptors, thereby forming a multivalent adhesive junction during parasite entry...
  20. ncbi request reprint Identification and partial characterization of a second Kazal inhibitor in Toxoplasma gondii
    Meredith Teilhet Morris
    The W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Mol Biochem Parasitol 128:119-22. 2003
  21. ncbi request reprint Is Toxoplasma egress the first step in invasion?
    Eleanor F Hoff
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe St, Baltimore, MD 21205, USA
    Trends Parasitol 18:251-5. 2002
    ....
  22. pmc Targeted deletion of MIC5 enhances trimming proteolysis of Toxoplasma invasion proteins
    Susannah D Brydges
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, MD 21205, USA
    Eukaryot Cell 5:2174-83. 2006
    ..Together, our findings suggest that MIC5 either directly regulates MPP2 activity or it influences MPP2's ability to access substrate cleavage sites on the parasite surface...
  23. ncbi request reprint Functional analysis of Toxoplasma gondii protease inhibitor 1
    Meredith Teilhet Morris
    The W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Biol Chem 277:45259-66. 2002
    ..TgPI-1 is the first anti-trypsin/chymotrypsin inhibitor to be identified in bradyzoites and sporozoites, stages of the parasite that would be exposed to proteolytic enzymes in the digestive tract of the host...
  24. ncbi request reprint The novel coccidian micronemal protein MIC11 undergoes proteolytic maturation by sequential cleavage to remove an internal propeptide
    Jill M Harper
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA
    Int J Parasitol 34:1047-58. 2004
    ..This is the first microneme protein characterised to date in the phylum Apicomplexa that possesses this unique structure and undergoes maturation by removal of an internal propeptide...
  25. ncbi request reprint Mutation of an unusual mitochondrial targeting sequence of SODB2 produces multiple targeting fates in Toxoplasma gondii
    Susannah D Brydges
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA
    J Cell Sci 116:4675-85. 2003
    ..In addition to having an unusual targeting sequence, TgSODB2 is only the second mitochondrially imported, iron-containing SOD to be described...
  26. ncbi request reprint Neospora caninum expresses an unusual single-domain Kazal protease inhibitor that is discharged into the parasitophorous vacuole
    Meredith Teilhet Morris
    W Harry Feinstein Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Int J Parasitol 34:693-701. 2004
    ..caninum lysate, one with a molecular mass approximately 11 kDa and another at approximately 20 kDa. This, along with mass spectrometry analysis of recombinant NcPI-S, suggests that the inhibitor is expressed as a dimer in the parasite...
  27. ncbi request reprint Analysis of the Sarcocystis neurona microneme protein SnMIC10: protein characteristics and expression during intracellular development
    Jessica S Hoane
    Department of Veterinary Science, University of Kentucky, 108 Gluck Equine Research Center, Lexington, KY 40546 0099, USA
    Int J Parasitol 33:671-9. 2003
    ..Identification and characterisation of additional S. neurona microneme antigens and comparisons to orthologues in other Apicomplexa could provide further insight into the functions that these proteins serve during invasion of host cells...