Karen C Carroll

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Rapid diagnostics for methicillin-resistant Staphylococcus aureus: current status
    Karen C Carroll
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Mol Diagn Ther 12:15-24. 2008
  2. pmc Contamination of equipment in emergency settings: An exploratory study with a targeted automated intervention
    Chidi Obasi
    Division of Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Ann Surg Innov Res 3:8. 2009
  3. ncbi request reprint Comparison of a commercial reversed passive latex agglutination assay to an enzyme immunoassay for the detection of Shiga toxin-producing Escherichia coli
    K C Carroll
    Department of Pathology, ARUP Laboratories, Inc University of Utah, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    Eur J Clin Microbiol Infect Dis 22:689-92. 2003
  4. pmc Laboratory diagnosis of lower respiratory tract infections: controversy and conundrums
    Karen C Carroll
    University of Utah School of Medicine and Diagnostic Infectious Diseases Laboratories, ARUP Laboratories, Inc, Salt Lake City, Utah, USA
    J Clin Microbiol 40:3115-20. 2002
  5. doi request reprint Biology of Clostridium difficile: implications for epidemiology and diagnosis
    Karen C Carroll
    Division of Medical Microbiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA
    Annu Rev Microbiol 65:501-21. 2011
  6. doi request reprint Tests for the diagnosis of Clostridium difficile infection: the next generation
    Karen C Carroll
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Anaerobe 17:170-4. 2011
  7. pmc Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of staphylococci and enterococci
    Karen C Carroll
    Division of Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    J Clin Microbiol 44:2072-7. 2006
  8. pmc Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterobacteriaceae
    Karen C Carroll
    Division of Medical Microbiology, The Johns Hopkins Hospital, Meyer B1 193, 600 N Wolfe St, Baltimore, MD 21287, USA
    J Clin Microbiol 44:3506-9. 2006
  9. pmc Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype
    Donna M Wolk
    University of Arizona, BIO5 Institute, Department of Pathology, 1501 N Campbell Ave, Tucson, AZ 85724 5059, USA
    J Clin Microbiol 47:3129-37. 2009
  10. doi request reprint Antimicrobial prescribing practices in response to different Clostridium difficile diagnostic methodologies
    Emily R M Sydnor
    Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Infect Control Hosp Epidemiol 32:1133-6. 2011

Detail Information

Publications92

  1. ncbi request reprint Rapid diagnostics for methicillin-resistant Staphylococcus aureus: current status
    Karen C Carroll
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Mol Diagn Ther 12:15-24. 2008
    ..Future technological advances are likely to see real-time assays that combine multiple gene targets for assessment of microbial identification, virulence detection, and mechanisms of resistance beyond mecA...
  2. pmc Contamination of equipment in emergency settings: An exploratory study with a targeted automated intervention
    Chidi Obasi
    Division of Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Ann Surg Innov Res 3:8. 2009
    ..The SUDS device allows for effective and durable decontamination of hospital equipment of varying sizes in the clinical area without disrupting patient care...
  3. ncbi request reprint Comparison of a commercial reversed passive latex agglutination assay to an enzyme immunoassay for the detection of Shiga toxin-producing Escherichia coli
    K C Carroll
    Department of Pathology, ARUP Laboratories, Inc University of Utah, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    Eur J Clin Microbiol Infect Dis 22:689-92. 2003
    ..Serotypes identified included: O157:H7 (n=8), O26 (n=2), O111 (n=1) and O45:H2 (n=1). The VTEC screen is easy to perform and comparable to the Meridian EHEC test for detection of Shiga toxin in clinical samples...
  4. pmc Laboratory diagnosis of lower respiratory tract infections: controversy and conundrums
    Karen C Carroll
    University of Utah School of Medicine and Diagnostic Infectious Diseases Laboratories, ARUP Laboratories, Inc, Salt Lake City, Utah, USA
    J Clin Microbiol 40:3115-20. 2002
  5. doi request reprint Biology of Clostridium difficile: implications for epidemiology and diagnosis
    Karen C Carroll
    Division of Medical Microbiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA
    Annu Rev Microbiol 65:501-21. 2011
    ..Future research is likely to build upon the advancements in phylogenetics to create novel strategies for diagnosis, treatment, and prevention...
  6. doi request reprint Tests for the diagnosis of Clostridium difficile infection: the next generation
    Karen C Carroll
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Anaerobe 17:170-4. 2011
    ..More studies are needed to assess the impact of molecular tests on treatment and nosocomial spread of Clostridium difficile infections...
  7. pmc Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of staphylococci and enterococci
    Karen C Carroll
    Division of Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    J Clin Microbiol 44:2072-7. 2006
    ..3% EA. All 36 vancomycin-resistant enterococci were detected by the Phoenix system. The Phoenix system compares favorably to traditional methods for the ID and AST of staphylococci and enterococci...
  8. pmc Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterobacteriaceae
    Karen C Carroll
    Division of Medical Microbiology, The Johns Hopkins Hospital, Meyer B1 193, 600 N Wolfe St, Baltimore, MD 21287, USA
    J Clin Microbiol 44:3506-9. 2006
    ..All six were flagged by the Phoenix system expert rules. The Phoenix system compares favorably to traditional methods for ID and AST of Enterobacteriaceae...
  9. pmc Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype
    Donna M Wolk
    University of Arizona, BIO5 Institute, Department of Pathology, 1501 N Campbell Ave, Tucson, AZ 85724 5059, USA
    J Clin Microbiol 47:3129-37. 2009
    ..The high-throughput PCR/ESI-MS assay should improve clinical management of staphylococcal infections...
  10. doi request reprint Antimicrobial prescribing practices in response to different Clostridium difficile diagnostic methodologies
    Emily R M Sydnor
    Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Infect Control Hosp Epidemiol 32:1133-6. 2011
    ..In addition, a significant proportion of patients with confirmed CDI were not treated according to recommended guidelines...
  11. pmc Evaluation of a new commercial TaqMan PCR assay for direct detection of the clostridium difficile toxin B gene in clinical stool specimens
    Paul D Stamper
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Clin Microbiol 47:3846-50. 2009
    ..difficile, the Prodesse ProGastro Cd assay did detect more toxigenic C. difficile isolates than the CCCNA...
  12. doi request reprint Comparison of two rapid assays for Clostridium difficile Common antigen and a C difficile toxin A/B assay with the cell culture neutralization assay
    Megan E Reller
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    Am J Clin Pathol 133:107-9. 2010
    ..The use of rapid assays as outlined could enhance timely diagnosis of C difficile...
  13. doi request reprint Targeted surveillance to identify children colonized with vancomycin-resistant Enterococcus in the pediatric intensive care unit
    Aaron M Milstone
    Department of Pediatrics, Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Infect Control Hosp Epidemiol 31:95-8. 2010
    ..Targeted surveillance captured 94% of VRE-colonized children and may be an effective strategy to identify VRE carriers and facilitate pediatric infection prevention strategies...
  14. pmc Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile
    Megan E Reller
    Division of Medical Microbiology, Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    J Clin Microbiol 45:3601-5. 2007
    ..difficile (where the best reference standard is essential), and (iii) in epidemiologic studies (where the availability of an isolate allows for strain typing and antimicrobial susceptibility testing)...
  15. pmc Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population
    Jason E Farley
    Johns Hopkins University, School of Nursing, 600 N Wolfe Street, Carnegie 346, Baltimore, MD 21287, USA
    J Clin Microbiol 46:743-6. 2008
    ..5% and 91.0%, respectively, and after secondary analysis using enrichment broth, they were 89.0% and 91.7%, respectively. Twenty-three of 42 false-positive PCR lysates contained methicillin-susceptible S. aureus...
  16. pmc Prevalence, risk factors, and molecular epidemiology of methicillin-resistant Staphylococcus aureus among newly arrested men in Baltimore, Maryland
    Jason E Farley
    School of Nursing, Johns Hopkins University, Baltimore, MD, USA
    Am J Infect Control 36:644-50. 2008
    ..Outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) within prison populations seemingly attest to its spread within the corrections industry; however, the extent of MRSA colonization on arrest is unknown...
  17. pmc C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens
    Criziel D Quinn
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 48:603-5. 2010
    ..Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested...
  18. pmc Comparative analysis of two broad-range PCR assays for pathogen detection in positive-blood-culture bottles: PCR-high-resolution melting analysis versus PCR-mass spectrometry
    Kevin Jeng
    Johns Hopkins University, Baltimore, MD, USA
    J Clin Microbiol 50:3287-92. 2012
    ....
  19. pmc Performance of the Phoenix bacterial identification system compared with disc diffusion methods for identifying extended-spectrum beta-lactamase, AmpC and KPC producers
    Mark A Fisher
    University of Utah, School of Medicine, Salt Lake City, UT, USA
    J Med Microbiol 58:774-8. 2009
    ..Overall, these results indicate that laboratories should use the Phoenix ESBL results only as an initial screen followed by confirmation with an alternative method...
  20. pmc Genotypic and phenotypic characterization of methicillin-susceptible Staphylococcus aureus isolates misidentified as methicillin-resistant Staphylococcus aureus by the BD GeneOhm MRSA assay
    Paul D Stamper
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    J Clin Microbiol 49:1240-4. 2011
    ..In our patient population, diagnostic and surveillance testing and subsequent infection control practices may be impacted by the frequency of these excision events when using the BD-MRSA PCR for MRSA detection...
  21. doi request reprint Discordance between Staphylococcus aureus nasal colonization and skin infections in children
    Aaron E Chen
    Department of Pediatrics, Johns Hopkins Medical Institutions, Baltimore, MD, USA
    Pediatr Infect Dis J 28:244-6. 2009
    ..aureus, but significant discordance between nasal and wound isolates. Recurrent skin and soft tissue infections were common but unrelated to baseline methicillin-resistant S. aureus nasal colonization status...
  22. doi request reprint Bordetella hinzii septicemia in association with Epstein-Barr virus viremia and an Epstein-Barr virus-associated diffuse large B-cell lymphoma
    Alexandra C Hristov
    Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD 21287, USA
    Diagn Microbiol Infect Dis 61:484-6. 2008
    ..Isolates were resistant to many antimicrobials. Resistance and diagnostic challenges complicated management and contributed to mortality...
  23. pmc Comparison of BACTEC PLUS blood culture media to BacT/Alert FA blood culture media for detection of bacterial pathogens in samples containing therapeutic levels of antibiotics
    Diane Flayhart
    The Johns Hopkins Hospital, Microbiology Laboratory, Baltimore, MD 21287, USA
    J Clin Microbiol 45:816-21. 2007
    ....
  24. pmc Fatal infection caused by Cupriavidus gilardii in a child with aplastic anemia
    Matthew Karafin
    Department of Pathology, Division of Medical Microbiology, The Johns Hopkins University School of Medicine and The Johns Hopkins Hospital, Baltimore, Maryland 21287 7093, USA
    J Clin Microbiol 48:1005-7. 2010
    ..Cupriavidus gilardii is a Gram-negative bacterium that has rarely been associated with human infections. We report a fatal case of sepsis caused by C. gilardii in a previously healthy 12-year-old female...
  25. pmc Community-associated methicillin-resistant Staphylococcus aureus strains in pediatric intensive care unit
    Aaron M Milstone
    Department of Pediatric Infectious Diseases, The Johns Hopkins University School of Medicine, 200 N Wolfe St, Rubenstein 3141, Baltimore, MD 21287, USA
    Emerg Infect Dis 16:647-55. 2010
    ..Epidemic CA-MRSA strains are becoming endemic to PICUs, can be transmitted to hospitalized children, and can cause invasive hospital-acquired infections. Further appraisal of MRSA control is needed...
  26. pmc Evaluation of BBL CHROMagar VanRE for detection of vancomycin-resistant Enterococci in rectal swab specimens
    Paul D Stamper
    Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine and The Johns Hopkins Hospital, Baltimore, Maryland, USA
    J Clin Microbiol 48:4294-7. 2010
    ..1% and 94.8% and 84.2% and 99.7%, respectively. Among our patient population, more vancomycin-resistant enterococci (VRE) were recovered with CVRE than BEAV...
  27. doi request reprint Outbreak of multidrug-resistant Serratia marcescens infection in a neonatal intensive care unit
    Lisa L Maragakis
    Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Infect Control Hosp Epidemiol 29:418-23. 2008
    ..We report the investigation and control of an outbreak of multidrug-resistant (MDR) S. marcescens infection at an NICU...
  28. doi request reprint Prevalence, risk factors, and molecular epidemiology of methicillin-resistant Staphylococcus aureus nasal and axillary colonization among psychiatric patients on admission to an academic medical center
    Jason E Farley
    Johns Hopkins University, School of Nursing, Department of Community and Public Health, Baltimore, MD 21205, USA
    Am J Infect Control 41:199-203. 2013
    ..Infection control data from psychiatric units and clinics are limited...
  29. pmc Methicillin-resistant Staphylococcus aureus colonization and risk of subsequent infection in critically ill children: importance of preventing nosocomial methicillin-resistant Staphylococcus aureus transmission
    Aaron M Milstone
    Department of Pediatrics, Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Clin Infect Dis 53:853-9. 2011
    ..Methicillin-resistant Staphylococcus aureus (MRSA) colonization is a predictor of subsequent infection in hospitalized adults. The risk of subsequent MRSA infections in hospitalized children colonized with MRSA is unknown...
  30. doi request reprint Unrecognized burden of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus carriage in the pediatric intensive care unit
    Aaron M Milstone
    Department of Pediatrics, Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Infect Control Hosp Epidemiol 29:1174-6. 2008
    ..This weekly surveillance enabled more accurate estimates of the incidence rates of MRSA and VRE carriage and led to an increased number of isolation-days for patients...
  31. pmc Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples
    Paul D Stamper
    Department of Pathology, Division of Medical Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Clin Microbiol 47:373-8. 2009
    ..The BD GeneOhm Cdiff assay performed well compared to a standard cell culture neutralization assay and to toxigenic culture for the detection of toxigenic C. difficile directly from fecal specimens...
  32. pmc Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares
    Diane Flayhart
    Division of Medical Microbiology, Meyer B1 193, The Johns Hopkins Hospital, 600 N Wolfe St, Baltimore, MD 21287, USA
    J Clin Microbiol 43:5536-40. 2005
    ....
  33. ncbi request reprint Staphylococcus aureus colonization among healthcare workers at a tertiary care hospital
    Cecilia P Johnston
    Division of Infectious Diseases, Department of Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA
    Infect Control Hosp Epidemiol 28:1404-7. 2007
    ..aureus (MRSA) was 2%. The incidence of MRSA colonization was extremely low. This study suggests that the risk of MRSA transmission to healthcare workers is low in a hospital where MRSA is endemic...
  34. pmc Sphingomonas paucimobilis bloodstream infections associated with contaminated intravenous fentanyl
    Lisa L Maragakis
    Division of Infectious Diseases, Department of Hospital Epidemiology and Infection Control, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Emerg Infect Dis 15:12-8. 2009
    ..These pharmacies should adopt more stringent quality-control measures, including prerelease product testing, when compounding and distributing large quantities of sterile preparations...
  35. pmc Clinical validation of the molecular BD GeneOhm StaphSR assay for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in positive blood cultures
    Paul D Stamper
    Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Johns Hopkins Hospital, Baltimore, Maryland 21287, USA
    J Clin Microbiol 45:2191-6. 2007
    ..The BD GeneOhm StaphSR assay appears to be a valuable diagnostic tool for quickly differentiating bacteremia caused by MSSA and MRSA from that caused by other gram-positive cocci...
  36. doi request reprint Risk factors for positive admission surveillance cultures for methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci in a neurocritical care unit
    Paras Minhas
    Department of Neurology, Johns Hopkins University School of Medicine, and Department of Hospital Epidemiology and Infection Control, Johns Hopkins Hospital, Baltimore, MD, USA
    Crit Care Med 39:2322-9. 2011
    ....
  37. doi request reprint Ochroconis gallopava infection in a patient with chronic granulomatous disease: case report and review of the literature
    Zina Meriden
    Department of Pathology, Division of Infectious Disease, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Med Mycol 50:883-9. 2012
    ..We also review the literature of human infections with Ochroconis to date, and discuss its microbiology to apprise both clinicians and laboratory personnel of this infrequently encountered but potentially aggressive pathogen...
  38. doi request reprint An evaluation of environmental decontamination with hydrogen peroxide vapor for reducing the risk of patient acquisition of multidrug-resistant organisms
    Catherine L Passaretti
    Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Clin Infect Dis 56:27-35. 2013
    ..Traditional cleaning strategies do not remove all environmental MDROs. We evaluated the environmental and clinical impact of hydrogen peroxide vapor (HPV) room disinfection...
  39. pmc Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin
    John R Ticehurst
    Division of Medical Microbiology, The Johns Hopkins Hospital Meyer B1 193, 600 North Wolfe St, Baltimore, Maryland 21287 7093, USA
    J Clin Microbiol 44:1145-9. 2006
    ..Over 6 months, our laboratories' expenses were US dollar 143,000 less than if CCNA alone had been performed on all 5,887 specimens...
  40. pmc Comparison of the BD GeneOhm VanR assay to culture for identification of vancomycin-resistant enterococci in rectal and stool specimens
    Paul D Stamper
    Department of Pathology, The Johns Hopkins University, Baltimore, MD 21287, USA
    J Clin Microbiol 45:3360-5. 2007
    ..However, patient samples testing only vanB positive should be confirmed by another method for the presence of VRE...
  41. pmc Evaluation of Bio-Rad MRSASelect agar for detection of methicillin-resistant Staphylococcus aureus directly from blood cultures
    Stefan Riedel
    The Johns Hopkins University, School of Medicine, Department of Pathology Division of Microbiology, Baltimore, MD 21224, USA
    J Clin Microbiol 48:2285-8. 2010
    ..Results were compared to organism identification by using standard laboratory methods. Confirming coagulase on pink isolates, the sensitivity and specificity were both 99%...
  42. pmc Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis
    Helen Won
    Johns Hopkins University Department of Emergency Medicine, 5801 Smith Avenue, Suite 3220, Davis Building, Baltimore, MD 21209, USA
    J Clin Microbiol 48:3410-3. 2010
    ..With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings...
  43. pmc Comparison of BD Bactec Plus Aerobic/F medium to VersaTREK Redox 1 blood culture medium for detection of Candida spp. in seeded blood culture specimens containing therapeutic levels of antifungal agents
    Stefan Riedel
    The Johns Hopkins University, School of Medicine, Department of Pathology Division of Microbiology, Johns Hopkins Bayview Medical Center, 4940 Eastern Avenue, Baltimore, MD 21224, USA
    J Clin Microbiol 49:1524-9. 2011
    ..In the presence of therapeutic levels of commonly used antifungal agents, the Bactec FX system demonstrated a significantly greater recovery of various Candida spp., as well as a shorter LTD...
  44. doi request reprint Blood cultures: key elements for best practices and future directions
    Stefan Riedel
    Division of Microbiology, Department of Pathology, Johns Hopkins Bayview Medical Center, 4940 Eastern Avenue, Baltimore, MD 21224, USA
    J Infect Chemother 16:301-16. 2010
    ....
  45. pmc Clostridium glycolicum wound infections: case reports and review of the literature
    Wei Jiang
    Department of Pathology, Division of Medical Microbiology, Johns Hopkins Hospital, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Clin Microbiol 47:1599-601. 2009
    ..Our report extends the spectrum of the diseases caused by C. glycolicum...
  46. ncbi request reprint Cholera-like diarrhea and shock associated with community-acquired methicillin-resistant Staphylococcus aureus (USA400 clone) pneumonia
    Allison Agwu
    Division of Pediatric Infectious Disease, Johns Hopkins University School of Medicine, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Room W5041, Baltimore, MD 21205, USA
    Pediatr Infect Dis J 26:271-3. 2007
    ..Shock, multiorgan system failure and death rapidly ensued. Influenza A was detected from her nasopharynx and CA-MRSA (USA400), positive for pvl, mecA, sea, sec and seh genes was cultured from a deep tracheal aspirate...
  47. pmc Randomized controlled trial of cephalexin versus clindamycin for uncomplicated pediatric skin infections
    Aaron E Chen
    Johns Hopkins University, Department of Pediatrics, Division of Pediatric Emergency Medicine, CMSC 144, 600 N Wolfe St, Baltimore, MD 21287, USA
    Pediatrics 127:e573-80. 2011
    ..We hypothesized that clindamycin would be superior to cephalexin (an antibiotic without MRSA activity) for treatment of these infections...
  48. pmc Interpretive criteria for use of AccuProbe for identification of Mycobacterium avium complex directly from 7H9 broth cultures
    Joann L Cloud
    ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Clin Microbiol 43:3474-8. 2005
    ..These data support the use of 80,000 RLU as the cutoff for a positive result in testing of 7H9 broth cultures with the MAC AccuProbe...
  49. pmc Rapid molecular genotyping and clonal complex assignment of Staphylococcus aureus isolates by PCR coupled to electrospray ionization-mass spectrometry
    Thomas A Hall
    Ibis Biosciences, Inc, 1896 Rutherford Rd, Carlsbad, California 92008, USA
    J Clin Microbiol 47:1733-41. 2009
    ..The PCR/ESI-MS method enables genotyping of over 180 samples of S. aureus per day in an automated fashion...
  50. pmc Does the piperacillin minimum inhibitory concentration for Pseudomonas aeruginosa influence clinical outcomes of children with pseudomonal bacteremia?
    Pranita D Tamma
    Division of Pediatric Infectious Diseases, Department of Pediatrics, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
    Clin Infect Dis 55:799-806. 2012
    ..Data on whether PK-PD modeling correlates with clinical outcomes in children are needed before resorting to broader classes of antibiotics to treat P. aeruginosa...
  51. doi request reprint Caveat emptor: the role of suboptimal bronchoscope repair practices by a third-party vendor in a pseudo-outbreak of pseudomonas in bronchoalveolar lavage specimens
    Sara E Cosgrove
    Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Infect Control Hosp Epidemiol 33:224-9. 2012
    ..To describe a pseudo-outbreak associated with loose bronchoscope biopsy ports caused by inadequate bronchoscope repair practices by third-party vendors and to alert healthcare personnel to assess bronchoscope repair practices...
  52. pmc Comparison of the BBL CHROMagar Staph aureus agar medium to conventional media for detection of Staphylococcus aureus in respiratory samples
    Diane Flayhart
    Division of Medical Microbiology, Meyer B1 193, The Johns Hopkins Hospital, 600 N Wolfe St, Baltimore, MD 21287 7093, USA
    J Clin Microbiol 42:3566-9. 2004
    ..aureus recovered from CSA compared to SBA or MSA. Our data support the use of CSA in place of standard culture media for detection of S. aureus in heavily contaminated respiratory samples...
  53. ncbi request reprint Serious bacterial infections in febrile infants 1 to 90 days old with and without viral infections
    Carrie L Byington
    Department of Pediatrics, University of Utah, Salt Lake City, Utah, USA
    Pediatrics 113:1662-6. 2004
    ..The occurrence of SBI in Rochester LR or HR infants with confirmed viral infections is unknown. The objective of this study was to determine the occurrence of SBI in Rochester LR and HR infants with and without viral infections...
  54. doi request reprint Daptomycin therapy failure in an adolescent with methicillin-resistant Staphylococcus aureus bacteremia
    Lara M Jacobson
    Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Pediatr Infect Dis J 28:445-7. 2009
    ..We report an adolescent patient with severe burn injuries who had persistent methicillin-resistant Staphylococcus aureus bacteremia and rapidly developed nonsusceptibility to daptomycin. We review the relevant literature...
  55. ncbi request reprint Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler PCR assay with inhibition control
    Joann L Cloud
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA
    Diagn Microbiol Infect Dis 46:189-95. 2003
    ..The described multiplex assay is a rapid, sensitive, contamination-limiting, real-time PCR assay that controls for inhibition. The assay performs well using liquid or swab samples and from dried material on slides...
  56. pmc Comparison of an automated repetitive sequence-based PCR microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus
    T L Ross
    The Johns Hopkins Hospital, Meyer B1 193, 600 N Wolfe St, Baltimore, MD 21287 7093, USA
    J Clin Microbiol 43:5642-7. 2005
    ....
  57. ncbi request reprint Staphylococcus aureus with reduced susceptibility to vancomycin
    S E Cosgrove
    Division of Infectious Disease, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Clin Infect Dis 39:539-45. 2004
    ..Next, we describe the mechanisms of resistance and methods of laboratory detection of the organisms. Finally, we address infection control and management issues associated with isolation of VISA and VRSA...
  58. pmc Epidemiology and outcomes of Clostridium difficile infections in hematopoietic stem cell transplant recipients
    Carolyn D Alonso
    Department of Medicine, The Johns Hopkins Hospital, Baltimore, MD 21205, USA
    Clin Infect Dis 54:1053-63. 2012
    ..A potentially important interplay between CDI and GVHD involving the gastrointestinal tract was observed...
  59. pmc Frequency of sample submission for optimal utilization of the cell culture cytotoxicity assay for detection of Clostridium difficile toxin
    Anita P Borek
    Division of Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287 7093, USA
    J Clin Microbiol 43:2994-5. 2005
    ..In only two cases (0.8%; n = 247) was a third sample positive when the first two samples were negative. In this study, submission of multiple samples for CCCA did not increase detection of Clostridium difficile infection...
  60. ncbi request reprint Human herpesvirus 6 infection in febrile infants ninety days of age and younger
    Carrie L Byington
    Department of Pediatrics, University of Utah, Salt Lake City, USA
    Pediatr Infect Dis J 21:996-9. 2002
    ..The importance of human herpesvirus 6 (HHV-6) as a pathogen in febrile infants </=90 days of age is unknown...
  61. ncbi request reprint Mycobacterium arupense sp. nov., a non-chromogenic bacterium isolated from clinical specimens
    Joann L Cloud
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Int J Syst Evol Microbiol 56:1413-8. 2006
    ..nov. is proposed. The type strain is AR30097(T) (=ATCC BAA-1242(T) = DSM 44942(T))...
  62. ncbi request reprint Use of strain typing data to estimate bacterial transmission rates in healthcare settings
    Brian R Jackson
    Department of Medical Informatics, University of Utah, Salt Lake City, Utah, USA
    Infect Control Hosp Epidemiol 26:638-45. 2005
    ..To create an affordable and accurate method for continuously monitoring bacterial transmission rates in healthcare settings...
  63. pmc Comparison of swab and sponge methodologies for identification of Acinetobacter baumannii from the hospital environment
    Kerri A Thom
    Department of Epidemiology and Public Health, University of Maryland School of Medicine, Baltimore, Maryland, USA
    J Clin Microbiol 50:2140-1. 2012
    ..The sensitivity of the swab method was 87%, while the sensitivity of the sponge method was 75%. Given the comparable results, use of the cheaper and less laborious swab technique is acceptable and may be preferable...
  64. pmc Comparison of the Denka-Seiken INFLU A.B-Quick and BD Directigen Flu A+B kits with direct fluorescent-antibody staining and shell vial culture methods for rapid detection of influenza viruses
    James J Dunn
    ARUP Laboratories, Inc, Salt Lake City, Utah 84108, USA
    J Clin Microbiol 41:2180-3. 2003
    ..5%; specificity, 99.6%; PPV, 90.9%; NPV, 97.5%), and the Directigen Flu A+B assay detected 9 of 16 influenza B viruses (sensitivity, 56.3%; specificity, 99.6%; PPV, 90%; NPV, 97.1%)...
  65. pmc Utility of extended blood culture incubation for isolation of Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella organisms: a retrospective multicenter evaluation
    Cathy A Petti
    Department of Medicine, University of Utah School of Medicine, Salt Lake City, USA
    J Clin Microbiol 44:257-9. 2006
    ..None of 407 blood cultures with extended incubation grew HACEK or other bacteria. Bacteremia from HACEK bacteria is rare, and extended incubation of blood cultures to recover HACEK bacteria is unnecessary...
  66. pmc Role of the clinical mycobacteriology laboratory in diagnosis and management of tuberculosis in low-prevalence settings
    Nicole M Parrish
    Johns Hopkins Medical Institutions, Baltimore, Maryland, Baltimore, Maryland, USA
    J Clin Microbiol 49:772-6. 2011
    ..Finally, gamma interferon release assays are preferred to the tuberculin skin test for screening certain at-risk populations, and new CDC guidelines are available that assist clinicians in their use...
  67. pmc Evaluation of partial 16S ribosomal DNA sequencing for identification of nocardia species by using the MicroSeq 500 system with an expanded database
    Joann L Cloud
    ARUP Institute for Clinical and Experimental Pathology, Department of Pathology, University of Utah, Salt Lake City, Utah, USA
    J Clin Microbiol 42:578-84. 2004
    ..With an expanded database, the MicroSeq 500 system for partial 16S rDNA was able to correctly identify the human pathogens N. brasiliensis, N. cyriacigeorgica, N. farcinica, N. nova, N. otitidiscaviarum, and N. veterana...
  68. ncbi request reprint Epidemiology of community-acquired methicillin-resistant Staphylococcus aureus skin infections among healthcare workers in an outpatient clinic
    Cecilia P Johnston
    Division of Infectious Diseases, Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD 21287, USA
    Infect Control Hosp Epidemiol 27:1133-6. 2006
    ..Cultures of environmental samples from multiple surfaces in the clinic grew toxin-producing CA-MRSA strains, suggesting fomites may play a role in the transmission of these strains of MRSA...
  69. pmc Impact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture
    Joann L Cloud
    Associated Regional and University Pathologists ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Clin Microbiol 40:3838-40. 2002
    ..The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis. Dacron and rayon swabs are an excellent choice for both PCR and culture...
  70. pmc Comparison of the Denka Seiken slide agglutination method to the quellung test for serogrouping of Streptococcus pneumoniae isolates
    Cheryl K Shutt
    Institute for Clinical and Experimental Pathology, LLC, ARUP Laboratories, Salt Lake City, Utah 84108 1221, USA
    J Clin Microbiol 42:1274-6. 2004
    ..The slide agglutination method compares favorably with the Pneumotest method and is easier to perform and to interpret...
  71. pmc Invasive fungal sinusitis caused by Scytalidium dimidiatum in a lung transplant recipient
    James J Dunn
    ARUP Laboratories, Inc, Salt Lake City, Utah, USA
    J Clin Microbiol 41:5817-9. 2003
    ..Following 6 months of voriconazole treatment, the patient remained radiographically and clinically stable for a short time before dying of respiratory failure precipitated by graft rejection...
  72. pmc Sensitivity of respiratory virus culture when screening with R-mix fresh cells
    James J Dunn
    ARUP Laboratories, Inc, Salt Lake City, Utah 84108, USA
    J Clin Microbiol 42:79-82. 2004
    ..The sensitivities of DFA testing and R-Mix cells for identification of influenza viruses were 70.5% and 96.7%, respectively. The R-Mix method detected influenza virus in 18 samples that were negative by DFA testing...
  73. ncbi request reprint Haemophilus influenzae serotype f purulent pericarditis: a cause of death in a child with Down syndrome
    Janis M Taube
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Diagn Microbiol Infect Dis 56:87-9. 2006
    ..We report the case of a child with Down syndrome and recent atrial and ventricular septal defect repair who died from Haemophilus influenzae serotype f pericarditis...
  74. ncbi request reprint Use of heat labile UNG in an RT-PCR assay for enterovirus detection
    Edward W Taggart
    Associated Regional and University Pathologists ARUP, Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Virol Methods 105:57-65. 2002
    ..The combined enzymological approach has potential application to a wide variety of assays requiring sensitive RNA detection and stringent contamination control, including those utilizing real time detection methods...
  75. pmc Influenza, Winter Olympiad, 2002
    Adi V Gundlapalli
    University of Utah School of Medicine, Salt Lake City, Utah, USA
    Emerg Infect Dis 12:144-6. 2006
    ..Influenza A/B was diagnosed in 36 of 188 patients, including 13 athletes. Prompt management limited the spread of this outbreak...
  76. pmc Comparison of sorbitol MacConkey agar and a two-step method which utilizes enzyme-linked immunosorbent assay toxin testing and a chromogenic agar to detect and isolate enterohemorrhagic Escherichia coli
    T J Novicki
    Department of Pathology, University of Utah School of Medicine and ARUP Laboratories, Salt Lake City, Utah 84113, USA
    J Clin Microbiol 38:547-51. 2000
    ..All but one were isolated during the months of May to September. The two-step method was found to be considerably more expensive than SMAC for both positive and negative samples...
  77. ncbi request reprint An epidemiological investigation of a sustained high rate of pediatric parapneumonic empyema: risk factors and microbiological associations
    Carrie L Byington
    Department of Pediatrics, University of Utah, Salt Lake City, UT, 84132, USA
    Clin Infect Dis 34:434-40. 2002
    ..0001 for each factor). The increasing incidence of empyema was associated with infection due to S. pneumoniae serotype 1, outpatient treatment with certain antibiotics, ibuprofen use, and varicella...
  78. ncbi request reprint Spotting the spirochete: rapid diagnosis of leptospirosis in two returned travelers
    Stephanie S Gelman
    Division of Infectious Diseases, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    J Travel Med 9:165-7. 2002
  79. pmc Trichuris vulpis recovered from a patient with chronic diarrhea and five dogs
    James J Dunn
    ARUP Laboratories, Inc, Salt Lake City, Utah 84108, USA
    J Clin Microbiol 40:2703-4. 2002
    ..Morphologically, T. vulpis ova resemble those of the human whipworm (T. trichiura) but are nearly twice their size...
  80. pmc Enhancement of the AMPLICOR enterovirus PCR test with a coprecipitant
    E W Taggart
    Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah, USA
    J Clin Microbiol 36:3408-9. 1998
    ..The background signal was not affected by the addition of Pellet Paint. These data support the utility of a coprecipitant in minimizing false-negative results...
  81. ncbi request reprint Comparison of the TOX A/B test to a cell culture cytotoxicity assay for the detection of Clostridium difficile in stools
    W E Aldeen
    Associated Regional and University Pathologists, Inc Salt Lake City, UT 84108, USA
    Diagn Microbiol Infect Dis 36:211-3. 2000
    ..It is sensitive enough to use as a stand-alone test for the detection of C. difficile toxin in laboratories that do not have cell culture cytotoxicity testing capability...
  82. ncbi request reprint Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens
    M Hindiyeh
    ARUP Laboratories and Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, USA
    Am J Clin Pathol 116:218-24. 2001
    ..Nevertheless, because of the excellent sensitivity and specificity, the Hexaplex assay may be valuable in the diagnosis of respiratory viral infections in immunocompromised patients...
  83. ncbi request reprint Evaluation of the roche AMPLICOR enterovirus PCR assay in the diagnosis of enteroviral central nervous system infections
    K C Carroll
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT, USA
    J Clin Virol 19:149-56. 2000
    ..While culture has been useful in the detection of patients with viral meningitis it is time-consuming and lacks sensitivity. Detection of viral nucleic acid in patient specimens has been demonstrated to improve enteroviral detection...
  84. pmc Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation
    J L Cloud
    Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA
    J Clin Microbiol 38:1709-12. 2000
    ..The legionella-specific PCR assay that is described demonstrates high sensitivity and high specificity for routine detection of legionellae in respiratory samples...
  85. pmc Identification of Mycobacterium spp. by using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries
    J L Cloud
    Associated Regional and University Pathologists ARUP, Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA
    J Clin Microbiol 40:400-6. 2002
    ....
  86. pmc Evaluation of the Abbott LCx ligase chain reaction assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted disease clinic population
    K C Carroll
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City 84132, USA
    J Clin Microbiol 36:1630-3. 1998
    ..trachomatis and N. gonorrhoeae when performed on urine or genital swab samples. Swab samples had better sensitivity than urine samples for the detection of both pathogens...
  87. doi request reprint In vitro production of panton-valentine leukocidin among strains of methicillin-resistant Staphylococcus aureus causing diverse infections
    Stephanie M Hamilton
    Veterans Affairs Medical Center, Boise, Idaho 83702, USA
    Clin Infect Dis 45:1550-8. 2007
    ..Greater than 75% of these strains carry the genes for Panton-Valentine leukocidin (PVL), suggesting that this toxin may mediate these severe infections. However, to date, studies have not provided evidence of toxin production...
  88. pmc Nocardia veterana as a pathogen in North American patients
    Patricia S Conville
    Microbiology Service, Department of Laboratory Medicine, Warren G Magnuson Clinical Center, National Institutes of Health, U S Department of Health and Human Services, Bethesda, Maryland 20892, USA
    J Clin Microbiol 41:2560-8. 2003
    ..veterana from the related species Nocardia africana and N. nova because of the very high degree of 16S rRNA gene similarity among them...
  89. ncbi request reprint Evaluation of RIDOM, MicroSeq, and Genbank services in the molecular identification of Nocardia species
    Alexander Mellmann
    Institut fur Hygiene, Universitatsklinikum Munster, 48149 Munster, Germany
    Int J Med Microbiol 293:359-70. 2003
    ..The RIDOM service (http://www.ridom-rdna.de/) is in the process of making available a comprehensive and high-quality database for bacterial identification purposes and provides excellent results for the majority of Nocardia isolates...
  90. pmc Timing of specimen collection for blood cultures from febrile patients with bacteremia
    Stefan Riedel
    University of Iowa Hospitals and Clinics, Department of Pathology Division of Microbiology, 200 Hawkins Drive, C 606 GH, Iowa City, IA 52242 1009, USA
    J Clin Microbiol 46:1381-5. 2008
    ..A subset analysis based on patient age, gender, white blood cell count and specific cause of bacteremia generally also failed to reveal any associations...
  91. ncbi request reprint Caspofungin in combination with itraconazole for the treatment of invasive aspergillosis in humans
    Michael A Rubin
    Clin Infect Dis 34:1160-1. 2002
  92. doi request reprint Incidence and prevalence of multidrug-resistant acinetobacter using targeted active surveillance cultures
    Lisa L Maragakis
    JAMA 299:2513-4. 2008