Research Topics
Genomes and GenesSpecies | Fred BunzSummaryAffiliation: Johns Hopkins University Country: USA Publications
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Publications
High-throughput sequencing and cancer geneticsFred Bunz
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, 1650 Orleans Street, Room 146, Baltimore, Maryland 21231, USA
Cell Cycle 1:320-1. 2002- The Chk2 tumor suppressor is not required for p53 responses in human cancer cellsPrasad V Jallepalli
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University, Baltimore, Maryland 21231, USA
J Biol Chem 278:20475-9. 2003..Our results indicate that Chk2 kinase is not required for p53 activation in human cells and explain why CHK2 and TP53 mutations can jointly occur in human tumors... 
A panel of isogenic human cancer cells suggests a therapeutic approach for cancers with inactivated p53Surojit Sur
The Howard Hughes Medical Institute and The Ludwig Center for Cancer Genetics and Therapeutics, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 106:3964-9. 2009..This hypothesis was validated by demonstrating that stressed cancer cells without WT TP53 alleles were highly sensitive to PLK1 inhibitors, both in vivo and in vitro...- Targeted mutations in the ATR pathway define agent-specific requirements for cancer cell growth and survivalDeborah Wilsker
Department of Radiation Oncology and Molecular Radiation Sciences and The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
Mol Cancer Ther 11:98-107. 2012..These results show that specifically inhibiting the Cdk2/ATR/Chk1 pathway via distinct regulators can differentially sensitize cancer cells to a wide range of therapeutic agents... - Essential function of Chk1 can be uncoupled from DNA damage checkpoint and replication controlDeborah Wilsker
Department of Radiation Oncology and Molecular Radiation Sciences and The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 105:20752-7. 2008..Our findings suggest an essential regulatory role for Chk1 phosphorylation during mitotic progression... - Loss of ataxia telangiectasia mutated- and Rad3-related function potentiates the effects of chemotherapeutic drugs on cancer cell survivalDeborah Wilsker
Department of Radiation Oncology and Molecular Radiation Sciences, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Mol Cancer Ther 6:1406-13. 2007..These results suggest that specific inhibition of ATR activity will be a valid strategy to increase the effectiveness of currently used modes of therapy... - Genetic knockouts and knockins in human somatic cellsCarlo Rago
Sidney Kimmel Comprehensive Cancer Center, Cellular and Molecular Medicine Program, Johns Hopkins University School of Medicine, 1550 Orleans Street CRB2 453, Baltimore, Maryland 21231, USA
Nat Protoc 2:2734-46. 2007..The protocol described herein allows the generation of a cell line with a single altered allele in 3 months. A second allele of the same gene can be targeted in an additional 3 months... - RNAi-mediated silencing of nuclear factor erythroid-2-related factor 2 gene expression in non-small cell lung cancer inhibits tumor growth and increases efficacy of chemotherapyAnju Singh
Department of Environmental Health Sciences, Division of Toxicology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
Cancer Res 68:7975-84. 2008..Thus, targeting Nrf2 activity in lung cancers, particularly those with Keap1 mutations, could be a promising strategy to inhibit tumor growth and circumvent chemoresistance... - X-linked inhibitor of apoptosis protein (XIAP) is a nonredundant modulator of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human cancer cellsJordan M Cummins
The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Cancer Res 64:3006-8. 2004..These results demonstrate that XIAP is a nonredundant modulator of TRAIL-mediated apoptosis and provide a rationale for XIAP as a therapeutic target... - Deficiency of Bloom syndrome helicase activity is radiomimeticDavid P Horowitz
Department of Radiation Oncology and Molecular Radiation Sciences, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Cancer Biol Ther 7:1783-6. 2008..These results suggest that pharmacological inhibitors of BLM would have a radiomimetic effect and that transient inhibition of BLM activity might be a viable strategy for anticancer therapy... - Checkpoint bypass and cell viabilityJon H Chung
Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Cell Cycle 9:2102-7. 2010.... - Chk1 phosphorylation during mitosis: a new role for a master regulatorDeborah Wilsker
Department of Radiation Oncology and Molecular Radiation Sciences, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Cell Cycle 8:1161-3. 2009..In contrast, the checkpoint kinase Chk1 and its upstream activator ATR are essential.(1-4) What do these proteins do in undamaged cells?.. - Targeted inactivation of p53 in human cells does not result in aneuploidyFred Bunz
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University, Baltimore, Maryland 21231, USA
Cancer Res 62:1129-33. 2002..Rates of sister chromatid exchange and homologous recombination were also unaffected by p53 status. These results show that inactivation of p53 does not, in and of itself, lead to the development of aneuploidy... - Improved methods for the generation of human gene knockout and knockin cell linesOzlem Topaloglu
Department of Radiation Oncology and Molecular Radiation Sciences, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
Nucleic Acids Res 33:e158. 2005..This versatile method was thus shown to facilitate the efficient integration of small, defined sequence alterations into the chromosomes of cultured human cells... - Gain of Nrf2 function in non-small-cell lung cancer cells confers radioresistanceAnju Singh
Department of Environmental Health Sciences, Johns Hopkins University, Baltimore, Maryland, USA
Antioxid Redox Signal 13:1627-37. 2010..Thus, targeting Nrf2 activity in radioresistant tumors could be a promising strategy to circumvent radioresistance... - Thymidylate synthase and 5-fluorouracil: a cautionary taleFred Bunz
Department of Radiation Oncology and Molecular Radiation Sciences, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Cancer Biol Ther 7:995-6. 2008 - Gene-specific selection against experimental fanconi anemia gene inactivation in human cancerEike Gallmeier
The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, Maryland, USA
Cancer Biol Ther 6:654-60. 2007..This would explain why certain FA gene defects, despite an apparent selection for FA pathway inactivation in cancer, are rarely observed in tumors among the general population... - ATM and ATR: components of an integrated circuitPaula J Hurley
Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
Cell Cycle 6:414-7. 2007..In this article, we focus on several new developments that give insight into the integrated processing of diverse signals that arise during the damage and replication of DNA... - Haploinsufficiency of hTERT leads to telomere dysfunction and radiosensitivity in human cancer cellsTravis Hauguel
Department of Radiation Oncology and Molecular Radiation Sciences and The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
Cancer Biol Ther 2:679-84. 2003..These results suggest that telomerase inhibitors might be useful in the sensitization of cancer cells to DNA damaging agents... - Overcoming the hypoxic barrier to radiation therapy with anaerobic bacteriaChetan Bettegowda
Howard Hughes Medical Institute and Sidney Kimmel Cancer Center, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 100:15083-8. 2003..C. novyi-NT spores added little toxicity to the radiotherapeutic regimens, and the combination resulted in long-term remissions in a significant fraction of animals... - Hematopoietic differentiation of human embryonic stem cells progresses through sequential hematoendothelial, primitive, and definitive stages resembling human yolk sac developmentElias T Zambidis
Division of Immunology and Hematopoiesis, Sidney Kimmel comprehensive Cancer Center at John Hokins, The John Hopkins School of Medicine, Baltimore, MD, USA
Blood 106:860-70. 2005.... - Digital HLA allelotypingOzlem Topaloglu
Department of Radiation Oncology and Molecular Radiation Sciences, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins School of Medicine, Baltimore, Maryland 21231, USA
Cancer Biol Ther 3:899-902. 2004..We present a novel method for HLA analysis, which we term Digital HLA Allelotyping, or dig-HLA. This method employs a strategy in which the expressed HLA alleles are randomly sorted and individually identified... - Human cell knockoutsFred Bunz
Radiobiology Program, Johns Hopkins Oncology Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
Curr Opin Oncol 14:73-8. 2002..The targeting of human genes promises to be a powerful tool in the characterization of the molecular pathways relevant to cancer... - p53 regulates mitochondrial respirationSatoaki Matoba
Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Science 312:1650-3. 2006..That SCO2 couples p53 to mitochondrial respiration provides a possible explanation for the Warburg effect and offers new clues as to how p53 might affect aging and metabolism... - Hypoxia-inducible factor-1alpha promotes nonhypoxia-mediated proliferation in colon cancer cells and xenograftsDuyen T Dang
Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical Center, 1150 West Medical Center Drive, Ann Arbor, MI 48109, USA
Cancer Res 66:1684-936. 2006....
Research Grants
- DNA Damage Signaling in Human Cancer CellsFred Bunz; Fiscal Year: 2007..The combination of the above studies should provide considerable insight into functions of DNA damage signal transduction pathways and their dysfunction in human cancers. ..