Seth Blackshaw

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. Newman E, Wu D, Taketo M, Zhang J, Blackshaw S. Canonical Wnt signaling regulates patterning, differentiation and nucleogenesis in mouse hypothalamus and prethalamus. Dev Biol. 2018;442:236-248 pubmed publisher
    ..These findings demonstrate a central and multifaceted role for canonical Wnt signaling in regulating growth, patterning, differentiation and nucleogenesis in multiple diencephalic regions. ..
  2. Bedont J, Legates T, Slat E, Byerly M, Wang H, Hu J, et al. Lhx1 controls terminal differentiation and circadian function of the suprachiasmatic nucleus. Cell Rep. 2014;7:609-22 pubmed publisher
    ..These animals provide a platform for studying the central oscillator's role in physiology and cognition. ..
  3. Yoo S, Blackshaw S. Regulation and function of neurogenesis in the adult mammalian hypothalamus. Prog Neurobiol. 2018;170:53-66 pubmed publisher
  4. Wang J, Zibetti C, Shang P, Sripathi S, Zhang P, Cano M, et al. ATAC-Seq analysis reveals a widespread decrease of chromatin accessibility in age-related macular degeneration. Nat Commun. 2018;9:1364 pubmed publisher
    ..Finally, overexpression of HDAC11 is partially responsible for the observed reduction in chromatin accessibility, suggesting that HDAC11 may be a potential new therapeutic target for AMD. ..
  5. Newman E, Kim D, Wan J, Wang J, Qian J, Blackshaw S. Foxd1 is required for terminal differentiation of anterior hypothalamic neuronal subtypes. Dev Biol. 2018;439:102-111 pubmed publisher
    ..These findings demonstrate that Foxd1 acts in hypothalamic progenitors to allow sustained expression of a subset of genes selectively expressed in mature neurons of the anterior hypothalamus. ..
  6. Ramos López P, Irizarry J, Pino I, Blackshaw S. Antibody Specificity Profiling Using Protein Microarrays. Methods Mol Biol. 2018;1785:223-229 pubmed publisher
    ..This protocol describes in detail the process of array pretreatment, antibody binding, washing, scanning and quantitative analysis of antibody specificity. ..
  7. Thein T, de Melo J, Zibetti C, Clark B, Juarez F, Blackshaw S. Control of lens development by Lhx2-regulated neuroretinal FGFs. Development. 2016;143:3994-4002 pubmed
    ..These data demonstrate that neuroretinal expression of Lhx2 and neuroretina-derived FGF factors are crucial for lens fiber development in vivo. ..
  8. Bedont J, Legates T, Buhr E, Bathini A, Ling J, Bell B, et al. An LHX1-Regulated Transcriptional Network Controls Sleep/Wake Coupling and Thermal Resistance of the Central Circadian Clockworks. Curr Biol. 2017;27:128-136 pubmed publisher
    ..These studies identify Lhx1 as the first gene required for temperature resistance of the SCN clockworks and demonstrate that acute light control of sleep is routed through the SCN and its immediate output regions. ..
  9. de Melo J, Clark B, Venkataraman A, Shiau F, Zibetti C, Blackshaw S. Ldb1- and Rnf12-dependent regulation of Lhx2 controls the relative balance between neurogenesis and gliogenesis in the retina. Development. 2018;145: pubmed publisher
    ..These findings show that control of Lhx2 function by Ldb1 and Rnf12 underpins the coordinated differentiation of neurons and Müller glia in postnatal retina. ..

More Information

Publications15

  1. Liu S, Zibetti C, Wan J, Wang G, Blackshaw S, Qian J. Assessing the model transferability for prediction of transcription factor binding sites based on chromatin accessibility. BMC Bioinformatics. 2017;18:355 pubmed publisher
    ..The prediction of TF binding is transferable across TFs and/or cell lines suggesting there are a set of universal "rules". A computational tool was developed to predict TF binding sites based on the universal "rules". ..
  2. Salvatierra J, Lee D, Zibetti C, Duran Moreno M, Yoo S, Newman E, et al. The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation. J Neurosci. 2014;34:16809-20 pubmed publisher
    ..These findings provide key insights into the transcriptional regulatory network specifying this still poorly characterized cell type. ..
  3. de Melo J, Zibetti C, Clark B, Hwang W, Miranda Angulo A, Qian J, et al. Lhx2 Is an Essential Factor for Retinal Gliogenesis and Notch Signaling. J Neurosci. 2016;36:2391-405 pubmed publisher
    ..These results indicate that Lhx2 not only directly regulates expression of Notch signaling pathway components, but also acts together with the gliogenic Notch pathway to drive MG specification and differentiation. ..
  4. Cox E, Hwang W, Uzoma I, Hu J, Guzzo C, Jeong J, et al. Global Analysis of SUMO-Binding Proteins Identifies SUMOylation as a Key Regulator of the INO80 Chromatin Remodeling Complex. Mol Cell Proteomics. 2017;16:812-823 pubmed publisher
  5. Clark B, Blackshaw S. Understanding the Role of lncRNAs in Nervous System Development. Adv Exp Med Biol. 2017;1008:253-282 pubmed publisher
    ..Altogether, this chapter highlights the emerging role of lncRNAs in the developing brain and sheds light on novel, RNA-mediated mechanisms by which nervous system development is controlled. ..
  6. de Melo J, Blackshaw S. In Vivo Electroporation of Developing Mouse Retina. Methods Mol Biol. 2018;1715:101-111 pubmed publisher
    ..This method can be utilized to perform gain of function or loss of function studies in the mouse. Experimental design is limited only by construct availability. ..