Seth Blackshaw

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation
    Seth Blackshaw
    Department of Neuroscience, Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    J Neurosci 30:14925-30. 2010
  2. pmc The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons
    Jimmy de Melo
    Department of Neuroscience, Johns Hopkins University School of Medicine, 733 N Broadway Avenue, Baltimore, MD 21287, USA
    Development 138:2325-36. 2011
  3. pmc Pias3-dependent SUMOylation controls mammalian cone photoreceptor differentiation
    Akishi Onishi
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Neurosci 13:1059-65. 2010
  4. pmc High-throughput RNA in situ hybridization in mouse retina
    Seth Blackshaw
    The Salomon H Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 935:215-26. 2013
  5. pmc We contain multitudes: the protean face of retinoblastoma
    Seth Blackshaw
    Departments of Neuroscience, Neurology, and Ophthalmology, Institute for Cell Engineering and Center for High Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cancer Cell 20:137-8. 2011
  6. pmc LHX2 is necessary for the maintenance of optic identity and for the progression of optic morphogenesis
    Achira Roy
    Tata Institute of Fundamental Research, Mumbai, India 400005
    J Neurosci 33:6877-84. 2013
  7. pmc The orphan nuclear hormone receptor ERRbeta controls rod photoreceptor survival
    Akishi Onishi
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Proc Natl Acad Sci U S A 107:11579-84. 2010
  8. pmc Identification of hypothalamic neuron-derived neurotrophic factor as a novel factor modulating appetite
    Mardi S Byerly
    Department of Physiology and Center for Metabolism and Obesity Research, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Am J Physiol Regul Integr Comp Physiol 304:R1085-95. 2013
  9. pmc Pias3-dependent SUMOylation directs rod photoreceptor development
    Akishi Onishi
    Department of Neuroscience, Johns Hopkins University School of Medicine, BRB 329, 733 North Broadway Avenue, Baltimore, MD 21287, USA
    Neuron 61:234-46. 2009
  10. doi request reprint Systematic characterization of protein-DNA interactions
    Zhi Xie
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Mol Life Sci 68:1657-68. 2011

Research Grants

  1. The role of PIAS3 in retinal development
    Seth Blackshaw; Fiscal Year: 2007
  2. The role of PIAS3 in retinal development
    Seth Blackshaw; Fiscal Year: 2010
  3. Transcriptional regulation of retinal cell differentiation and function
    Seth Blackshaw; Fiscal Year: 2011
  4. The role of PIAS3 in retinal development
    Seth Blackshaw; Fiscal Year: 2009

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation
    Seth Blackshaw
    Department of Neuroscience, Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    J Neurosci 30:14925-30. 2010
    ..Finally, we evaluate the molecular mechanisms involved in the experience-dependent development of both thalamo-cortical and hypothalamic neural circuitry...
  2. pmc The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons
    Jimmy de Melo
    Department of Neuroscience, Johns Hopkins University School of Medicine, 733 N Broadway Avenue, Baltimore, MD 21287, USA
    Development 138:2325-36. 2011
    ....
  3. pmc Pias3-dependent SUMOylation controls mammalian cone photoreceptor differentiation
    Akishi Onishi
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Neurosci 13:1059-65. 2010
    ....
  4. pmc High-throughput RNA in situ hybridization in mouse retina
    Seth Blackshaw
    The Salomon H Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 935:215-26. 2013
    ..Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections...
  5. pmc We contain multitudes: the protean face of retinoblastoma
    Seth Blackshaw
    Departments of Neuroscience, Neurology, and Ophthalmology, Institute for Cell Engineering and Center for High Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cancer Cell 20:137-8. 2011
    ..reveal that retinoblastomas are highly homogeneous at the molecular level and coexpress genes characteristic of retinal progenitors and various different mature retinal cell types, while ultrastructurally resembling amacrine cells...
  6. pmc LHX2 is necessary for the maintenance of optic identity and for the progression of optic morphogenesis
    Achira Roy
    Tata Institute of Fundamental Research, Mumbai, India 400005
    J Neurosci 33:6877-84. 2013
    ..These findings provide new insight into the anophthalmic phenotype of the Lhx2 mutant and reveal novel roles for this transcription factor in eye development...
  7. pmc The orphan nuclear hormone receptor ERRbeta controls rod photoreceptor survival
    Akishi Onishi
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Proc Natl Acad Sci U S A 107:11579-84. 2010
    ..Our findings demonstrate that ERRbeta is a critical regulator of rod photoreceptor function and survival, and suggest that ERRbeta agonists may be useful in the treatment of certain retinal dystrophies...
  8. pmc Identification of hypothalamic neuron-derived neurotrophic factor as a novel factor modulating appetite
    Mardi S Byerly
    Department of Physiology and Center for Metabolism and Obesity Research, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Am J Physiol Regul Integr Comp Physiol 304:R1085-95. 2013
    ..Our study validates the power of using an integrated experimental and bioinformatic approach to identify novel CNS-derived proteins with appetite-modulating function and reveals NENF as an important central modulator of food intake...
  9. pmc Pias3-dependent SUMOylation directs rod photoreceptor development
    Akishi Onishi
    Department of Neuroscience, Johns Hopkins University School of Medicine, BRB 329, 733 North Broadway Avenue, Baltimore, MD 21287, USA
    Neuron 61:234-46. 2009
    ..Our data thus identify Pias3-mediated SUMOylation of photoreceptor-specific transcription factors as a key mechanism of rod specification...
  10. doi request reprint Systematic characterization of protein-DNA interactions
    Zhi Xie
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cell Mol Life Sci 68:1657-68. 2011
    ..The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks...
  11. pmc Construction of human activity-based phosphorylation networks
    Robert H Newman
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Syst Biol 9:655. 2013
    ..Overall, these studies provide global insights into kinase-mediated signaling pathways and promise to advance our understanding of cellular signaling processes in humans...
  12. doi request reprint Characterization of protein-DNA interactions using protein microarrays
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cold Spring Harb Protoc 2011:pdb.prot5614. 2011
    ..This approach provides simultaneous identification of PDIs for thousands of proteins, and multiple carefully designed DNA probes can be tested in parallel, which enables a rapid mapping of PDIs on a proteome-wide scale...
  13. pmc Injury-independent induction of reactive gliosis in retina by loss of function of the LIM homeodomain transcription factor Lhx2
    Jimmy de Melo
    Departments of Neuroscience, Center for High Throughput Biology, and Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 109:4657-62. 2012
    ..We provide in vivo evidence that Lhx2 actively maintains mature Müller glia in a nonreactive state, with loss of function initiating a specific program of nonproliferative hypertrophic gliosis...
  14. pmc The long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity
    Nicole A Rapicavoli
    Department of Neuroscience, Neurology and Ophthalmology, Center for High Throughput Biology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Neural Dev 6:32. 2011
    ..Thousands of different long non-coding RNAs are expressed during embryonic development, but the function of these molecules remains largely unexplored...
  15. pmc Functional protein microarray technology
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Wiley Interdiscip Rev Syst Biol Med 3:255-68. 2011
    ..We strongly believe that functional protein microarrays will soon become an indispensible and invaluable tool in proteomics research and systems biology...
  16. ncbi request reprint Lhx1 controls terminal differentiation and circadian function of the suprachiasmatic nucleus
    Joseph L Bedont
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Cell Rep 7:609-22. 2014
    ..These animals provide a platform for studying the central oscillator's role in physiology and cognition. ..
  17. pmc Tanycytes of the hypothalamic median eminence form a diet-responsive neurogenic niche
    Daniel A Lee
    Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Neurosci 15:700-2. 2012
    ..Blocking this neurogenesis altered the weight and metabolic activity of adult mice. These findings reveal a previously unreported neurogenic niche in the mammalian hypothalamus with important implications for metabolism...
  18. pmc Profiling the human protein-DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling
    Shaohui Hu
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 139:610-22. 2009
    ..One such uDBP, ERK2, acts as a transcriptional repressor for interferon gamma-induced genes, suggesting important biological roles for such proteins...
  19. pmc Estrogen-related receptor β deficiency alters body composition and response to restraint stress
    Mardi S Byerly
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    BMC Physiol 13:10. 2013
    ..To further understand the biological role of Errβ, we characterized the stress response of mice deficient for one or both alleles of Errβ...
  20. pmc RNA toxicity from the ALS/FTD C9ORF72 expansion is mitigated by antisense intervention
    Christopher J Donnelly
    Department of Neurology, Johns Hopkins University, 855 N Wolfe Street, Rangos 2 270, Baltimore, MD 21205, USA Brain Science Institute, Johns Hopkins University, 855 N Wolfe Street, Rangos 2 270, Baltimore, MD 21205, USA
    Neuron 80:415-28. 2013
    ..These data indicate a toxic RNA gain-of-function mechanism as a cause of C9ORF72 ALS and provide candidate antisense therapeutics and candidate human pharmacodynamic markers for therapy. ..
  21. pmc Estrogen-related receptor β deletion modulates whole-body energy balance via estrogen-related receptor γ and attenuates neuropeptide Y gene expression
    Mardi S Byerly
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Eur J Neurosci 37:1033-47. 2013
    ..Overall, the relative expression ratio between ERRβ and ERRγ may be important in modulating ingestive behavior, specifically satiety, gene expression, as well as whole-body energy balance...
  22. pmc Rapid identification of monospecific monoclonal antibodies using a human proteome microarray
    Jun Seop Jeong
    Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Mol Cell Proteomics 11:O111.016253. 2012
    ..Our data indicate that the pipeline provides a robust platform for the generation of monoclonal antibodies of exceptional specificity...
  23. pmc Three-dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain
    Manisha Aggarwal
    Russell H Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Magn Reson Med 64:249-61. 2010
    ....
  24. doi request reprint Vertebrate retina and hypothalamus development
    Mardi S Byerly
    Department of Neuroscience, Neurology and Ophthalamology, Johns Hopkins School of Medicine, Baltimore, MD, USA
    Wiley Interdiscip Rev Syst Biol Med 1:380-9. 2009
    ....
  25. pmc The long noncoding RNA RNCR2 directs mouse retinal cell specification
    Nicole A Rapicavoli
    Department of Neuroscience, Neurology and Ophthalmology, Center for High Throughput Biology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, 733 N, Broadway Avenue, Baltimore, MD, USA
    BMC Dev Biol 10:49. 2010
    ..Despite their abundance, the function of these ncRNAs has remained largely unexplored. We have investigated the highly abundant lncRNA RNCR2 in regulation of mouse retinal cell differentiation...
  26. pmc WIDE AWAKE mediates the circadian timing of sleep onset
    Sha Liu
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA
    Neuron 82:151-66. 2014
    ..We propose that WAKE acts as a clock output molecule specifically for sleep, inhibiting LNvs at dusk to promote the transition from wake to sleep...
  27. pmc Stage-specific inhibition of TrkB activity leads to long-lasting and sexually dimorphic effects on body weight and hypothalamic gene expression
    Mardi S Byerly
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America Center for Metabolism and Obesity Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 8:e80781. 2013
    ....
  28. pmc Muscleblind-like 2: circadian expression in the mammalian pineal gland is controlled by an adrenergic-cAMP mechanism
    Jong So Kim
    Program on Developmental Endocrinology and Genetics, Section on Neuroendocrinology, the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    J Neurochem 110:756-64. 2009
    ..Mbnl2 protein exhibits a similar rhythm. In vitro studies indicate that the abundance of Mbnl2 transcripts and protein are controlled by an adrenergic/cAMP mechanism...
  29. ncbi request reprint D-aspartate regulates melanocortin formation and function: behavioral alterations in D-aspartate oxidase-deficient mice
    Alex S Huang
    Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 26:2814-9. 2006
    ..Therefore, Ddo is the endogenous enzyme that degrades D-aspartate, and Ddo-enriched organs, low in D-aspartate, may represent areas of high turnover where D-aspartate may be physiologically important...
  30. pmc Rax-CreERT2 Knock-In Mice: A Tool for Selective and Conditional Gene Deletion in Progenitor Cells and Radial Glia of the Retina and Hypothalamus
    Thomas Pak
    Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 9:e90381. 2014
    ..We anticipate that this line will prove useful for cell lineage analysis and investigation of gene function in the developing and mature retina, hypothalamus and pituitary. ..
  31. pmc Rax regulates hypothalamic tanycyte differentiation and barrier function in mice
    Ana L Miranda-Angulo
    Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21287 Institute of Medical Research, Universidad de Antioquia, School of Medicine, Medellin, Colombia 05001000
    J Comp Neurol 522:876-99. 2014
    ..In conclusion, we have provided evidence that Rax is required for the normal differentiation and patterning of hypothalamic tanycytes and ependymal cells, as well as for maintenance of the cerebrospinal fluid-hypothalamus barrier...
  32. pmc Botch promotes neurogenesis by antagonizing Notch
    Zhikai Chi
    Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 22:707-20. 2012
    ..Understanding the function of Botch expands our knowledge regarding both the regulation of Notch signaling and the complex signaling mediating neuronal development...
  33. pmc Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis
    Mary Katherine Tarrant
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Chem Biol 8:262-9. 2012
    ..This study suggests how a promiscuous protein kinase can be regulated at multiple levels to achieve particular biological outputs...
  34. ncbi request reprint New meaning in the message: noncoding RNAs and their role in retinal development
    Nicole A Rapicavoli
    Department of Neuroscience, Neurology and Ophthalmology, Center for High Throughput Biology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Dev Dyn 238:2103-14. 2009
    ....
  35. ncbi request reprint Serial analysis of gene expression (SAGE): experimental method and data analysis
    Seth Blackshaw
    Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Curr Protoc Hum Genet . 2007
    ..Software tools have also been specifically adapted for SAGE tags to allow cluster analysis of both public and user-generated data...
  36. pmc hPDI: a database of experimental human protein-DNA interactions
    Zhi Xie
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Bioinformatics 26:287-9. 2010
    ..Users can browse, search and download a subset or the entire data via a web interface. This database is freely accessible for any academic purposes...

Research Grants6

  1. The role of PIAS3 in retinal development
    Seth Blackshaw; Fiscal Year: 2007
    ..Moreover, mutations in rod-enriched transcription factors very often lead to photoreceptor degeneration and blindness, and we anticipate that this may also hold for Pias3. ..
  2. The role of PIAS3 in retinal development
    Seth Blackshaw; Fiscal Year: 2010
    ..Moreover, mutations in rod-enriched transcription factors very often lead to photoreceptor degeneration and blindness, and we anticipate that this may also hold for Pias3. ..
  3. Transcriptional regulation of retinal cell differentiation and function
    Seth Blackshaw; Fiscal Year: 2011
    ..We will also investigate the functional consequences of Lhx2 deletion in mature Muller glia, and determine whether constitutive glial reactivity regulates photoreceptor survival following retinal injury. ..
  4. The role of PIAS3 in retinal development
    Seth Blackshaw; Fiscal Year: 2009
    ..Moreover, mutations in rod-enriched transcription factors very often lead to photoreceptor degeneration and blindness, and we anticipate that this may also hold for Pias3. ..