Stephen Baylin

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. ncbi request reprint Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer
    S B Baylin
    The Johns Hopkins Comprehensive Cancer Center and Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Hum Mol Genet 10:687-92. 2001
  2. pmc A stem cell-like chromatin pattern may predispose tumor suppressor genes to DNA hypermethylation and heritable silencing
    Joyce E Ohm
    Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center
    Nat Genet 39:237-42. 2007
  3. ncbi request reprint Methylation-induced silencing of ASC/TMS1, a pro-apoptotic gene, is a late-stage event in colorectal cancer
    Marco A Riojas
    Department of Surgery, Johns Hopkins University, Baltimore, Maryland, USA
    Cancer Biol Ther 6:1710-6. 2007
  4. pmc Double strand breaks can initiate gene silencing and SIRT1-dependent onset of DNA methylation in an exogenous promoter CpG island
    Heather M O'Hagan
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, United States of America
    PLoS Genet 4:e1000155. 2008
  5. pmc DNMT1 modulates gene expression without its catalytic activity partially through its interactions with histone-modifying enzymes
    Eriko G Clements
    Department of Oncology and The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Nucleic Acids Res 40:4334-46. 2012
  6. pmc Loss of a single Hic1 allele accelerates polyp formation in Apc(Δ716) mice
    H P Mohammad
    The Sidney Kimmel Comprehensive Cancer Institute at Johns Hopkins, Johns Hopkins University, Baltimore, MD, USA
    Oncogene 30:2659-69. 2011
  7. pmc Alterations of immune response of Non-Small Cell Lung Cancer with Azacytidine
    John Wrangle
    The Johns Hopkins University, School of Medicine, Oncology Center Hematology Medical Oncology, Baltimore, Maryland
    Oncotarget 4:2067-79. 2013
  8. pmc Cigarette smoke induces epithelial to mesenchymal transition and increases the metastatic ability of breast cancer cells
    Francescopaolo Di Cello
    Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
    Mol Cancer 12:90. 2013
  9. pmc The cancer epigenome: its origins, contributions to tumorigenesis, and translational implications
    Stephen B Baylin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
    Proc Am Thorac Soc 9:64-5. 2012
  10. ncbi request reprint DNA methylation and gene silencing in cancer
    Stephen B Baylin
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Nat Clin Pract Oncol 2:S4-11. 2005

Research Grants

Collaborators

Detail Information

Publications72

  1. ncbi request reprint Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer
    S B Baylin
    The Johns Hopkins Comprehensive Cancer Center and Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Hum Mol Genet 10:687-92. 2001
    ..In the translational arena, the promoter hypermethylation changes hold great promise as DNA tumor markers and their potentially reversible state creates a target for cancer therapeutic strategies involving gene reactivation...
  2. pmc A stem cell-like chromatin pattern may predispose tumor suppressor genes to DNA hypermethylation and heritable silencing
    Joyce E Ohm
    Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center
    Nat Genet 39:237-42. 2007
    ....
  3. ncbi request reprint Methylation-induced silencing of ASC/TMS1, a pro-apoptotic gene, is a late-stage event in colorectal cancer
    Marco A Riojas
    Department of Surgery, Johns Hopkins University, Baltimore, Maryland, USA
    Cancer Biol Ther 6:1710-6. 2007
    ..Methylation of ASC/TMS1 may have implications for cancer prognosis...
  4. pmc Double strand breaks can initiate gene silencing and SIRT1-dependent onset of DNA methylation in an exogenous promoter CpG island
    Heather M O'Hagan
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, United States of America
    PLoS Genet 4:e1000155. 2008
    ..Furthermore, with contribution of the stress-related protein SIRT1, the break can lead to the onset of aberrant CpG island DNA methylation, which is frequently associated with tight gene silencing in cancer...
  5. pmc DNMT1 modulates gene expression without its catalytic activity partially through its interactions with histone-modifying enzymes
    Eriko G Clements
    Department of Oncology and The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Nucleic Acids Res 40:4334-46. 2012
    ....
  6. pmc Loss of a single Hic1 allele accelerates polyp formation in Apc(Δ716) mice
    H P Mohammad
    The Sidney Kimmel Comprehensive Cancer Institute at Johns Hopkins, Johns Hopkins University, Baltimore, MD, USA
    Oncogene 30:2659-69. 2011
    ....
  7. pmc Alterations of immune response of Non-Small Cell Lung Cancer with Azacytidine
    John Wrangle
    The Johns Hopkins University, School of Medicine, Oncology Center Hematology Medical Oncology, Baltimore, Maryland
    Oncotarget 4:2067-79. 2013
    ..Our studies define a biomarker strategy for response in a recently initiated trial to examine the potential of epigenetic therapy to sensitize patients with NSCLC to PD-1 immune checkpoint blockade. ..
  8. pmc Cigarette smoke induces epithelial to mesenchymal transition and increases the metastatic ability of breast cancer cells
    Francescopaolo Di Cello
    Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
    Mol Cancer 12:90. 2013
    ..In this work we have set out to investigate the progressive transforming effects of tobacco smoke on non-tumorigenic mammary epithelial cells and breast cancer cells using in vitro and in vivo models of chronic cigarette smoke exposure...
  9. pmc The cancer epigenome: its origins, contributions to tumorigenesis, and translational implications
    Stephen B Baylin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
    Proc Am Thorac Soc 9:64-5. 2012
    ..We can use all of this basic information for translational purposes in terms of deriving biomarkers for cancer risk states and detection and therapeutic strategies...
  10. ncbi request reprint DNA methylation and gene silencing in cancer
    Stephen B Baylin
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Nat Clin Pract Oncol 2:S4-11. 2005
    ..This review examines alteration in DNA methylation in cancer, effects on gene expression, and implications for the use of hypomethylating agents in the treatment of cancer...
  11. pmc A decade of exploring the cancer epigenome - biological and translational implications
    Stephen B Baylin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Nat Rev Cancer 11:726-34. 2011
    ..The enzymatic processes that control the epigenome present new opportunities for deriving therapeutic strategies designed to reverse transcriptional abnormalities that are inherent to the cancer epigenome...
  12. ncbi request reprint Mechanisms underlying epigenetically mediated gene silencing in cancer
    Stephen B Baylin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Bunting Blaustein Cancer Research Building, 1650 Orleans Street, Suite 541, Baltimore, MD 21231, USA
    Semin Cancer Biol 12:331-7. 2002
    ..This chapter reviews the progress in these arenas...
  13. ncbi request reprint Altered methylation patterns in cancer cell genomes: cause or consequence?
    Stephen Baylin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Cancer Cell 1:299-305. 2002
    ..The goal is to highlight important controversies in the field which can be productive targets of ongoing and future research...
  14. ncbi request reprint Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction?
    Stephen B Baylin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Bunting Blaustein Cancer Research Building, 1650 Orleans Street, Suite 530, Baltimore, Maryland 21231, USA
    Nat Rev Cancer 6:107-16. 2006
    ..Strategies to reverse epigenetic gene silencing might therefore be useful in cancer prevention and therapy...
  15. pmc Extraction and processing of circulating DNA from large sample volumes using methylation on beads for the detection of rare epigenetic events
    Brian Keeley
    Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, United States
    Clin Chim Acta 425:169-75. 2013
    ..The combination of improved recovery and sensitivity make possible the detection of rare epigenetic events and the development of high sensitivity epigenetic diagnostic assays...
  16. ncbi request reprint Combined DNA methyltransferase and histone deacetylase inhibition in the treatment of myeloid neoplasms
    Steven D Gore
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21287, USA
    Cancer Res 66:6361-9. 2006
    ..The promising percentage of major hematologic responses justifies the testing of such combinations in prospective randomized trials...
  17. pmc A murine xenograft model of spontaneous metastases of human lung adenocarcinoma
    James E Harris
    Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
    J Surg Res 171:e75-9. 2011
    ..Tail vein injection creates a pattern of metastases, but is artificial. We hypothesized that the liver is a convenient alternative xenograft site and that metastases would gradually proceed spontaneously...
  18. pmc Defining a chromatin pattern that characterizes DNA-hypermethylated genes in colon cancer cells
    Kelly M McGarvey
    Divisions of Cancer Biology, The Johns Hopkins Kimmel Cancer Center, Baltimore, Maryland 21231, USA
    Cancer Res 68:5753-9. 2008
    ..Our data have great relevance for the increasing interest in reexpression of DNA-hypermethylated genes for the treatment of cancer...
  19. pmc The epigenomics of cancer
    Peter A Jones
    Department of Urology, Biochemistry, and Molecular Biology, USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA
    Cell 128:683-92. 2007
    ....
  20. ncbi request reprint hTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site
    Rebekah L Zinn
    Sidney Kimmel Comprehensive Cancer Center and Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Cancer Res 67:194-201. 2007
    ....
  21. ncbi request reprint The cancer epigenome--components and functional correlates
    Angela H Ting
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Genes Dev 20:3215-31. 2006
    ..This possibility, coupled with the reversible nature of epigenetics, has enormous significance for the prevention and control of cancer...
  22. pmc Dysfunctional KEAP1-NRF2 interaction in non-small-cell lung cancer
    Anju Singh
    Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS Med 3:e420. 2006
    ..Increased expression of cellular antioxidants and xenobiotic detoxification enzymes has been implicated in resistance of tumor cells against chemotherapeutic drugs...
  23. ncbi request reprint Delayed diagnosis and elevated mortality in an urban population with HIV and lung cancer: implications for patient care
    Malcolm V Brock
    Johns Hopkins Hospital, and Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21287, USA
    J Acquir Immune Defic Syndr 43:47-55. 2006
    ..Lung cancer is more common in HIV-infected patients than in the general population. We examined how effectively lung cancer was being diagnosed in our HIV-infected patients...
  24. ncbi request reprint Hypermethylation of the GATA gene family in esophageal cancer
    Mingzhou Guo
    Cancer Biology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231 1000, USA
    Int J Cancer 119:2078-83. 2006
    ..Our studies demonstrate frequent silencing of GATA-4 and GATA-5, but not GATA-6, in human esophageal neoplasia associated with gene promoter hypermethylation...
  25. ncbi request reprint CIMPle origin for promoter hypermethylation in colorectal cancer?
    Kornel Schuebel
    Nat Genet 38:738-40. 2006
  26. pmc Stem cell chromatin patterns: an instructive mechanism for DNA hypermethylation?
    Joyce E Ohm
    Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University Medical Institutions, Baltimore, Maryland, USA
    Cell Cycle 6:1040-3. 2007
    ....
  27. pmc Inhibition of lysine-specific demethylase 1 by polyamine analogues results in reexpression of aberrantly silenced genes
    Yi Huang
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Bunting Blaustein Cancer Research Building, 1650 Orleans Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 104:8023-8. 2007
    ..We thus define important new agents for reversing aberrant repression of gene transcription...
  28. pmc Cancer as a manifestation of aberrant chromatin structure
    Malcolm V Brock
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21205 2196, USA
    Cancer J 13:3-8. 2007
    ..A more complete understanding of these key, regulatory events is vital in approaching a more rational drug therapy to various malignancies...
  29. pmc Convergence of mutation and epigenetic alterations identifies common genes in cancer that predict for poor prognosis
    Timothy A Chan
    Cancer Biology Program, The Johns Hopkins Kimmel Cancer Center, Baltimore, Maryland, United States of America
    PLoS Med 5:e114. 2008
    ..We hypothesize that key tumor suppressor genes in cancer may be subject to mutation or hypermethylation...
  30. pmc Epigenetic inactivation of the canonical Wnt antagonist SRY-box containing gene 17 in colorectal cancer
    Wei Zhang
    Division of Cancer Biology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
    Cancer Res 68:2764-72. 2008
    ....
  31. pmc A requirement for DICER to maintain full promoter CpG island hypermethylation in human cancer cells
    Angela H Ting
    Genomic Medicine Institute, Lerner Research Institute, Cleveland, Ohio, USA
    Cancer Res 68:2570-5. 2008
    ..This reactivation is associated with a dramatic loss of localized promoter DNA hypermethylation. Thus, intact DICER is required to maintain full promoter DNA hypermethylation of select epigenetically silenced loci in human cancer cells...
  32. pmc Cooperation between the Hic1 and Ptch1 tumor suppressors in medulloblastoma
    Kimberly J Briggs
    The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland 21231, USA
    Genes Dev 22:770-85. 2008
    ....
  33. ncbi request reprint Heterozygous disruption of Hic1 predisposes mice to a gender-dependent spectrum of malignant tumors
    Wen Yong Chen
    Division of Tumor Biology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Medical Institutions, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Nat Genet 33:197-202. 2003
    ..We conclude that HIC1 is a candidate tumor-suppressor gene for which loss of function in both mouse and human cancers is associated only with epigenetic modifications...
  34. doi request reprint DNA methylation markers and early recurrence in stage I lung cancer
    Malcolm V Brock
    Johns Hopkins Hospital, Baltimore, USA
    N Engl J Med 358:1118-28. 2008
    ..Despite optimal and early surgical treatment of non-small-cell lung cancer (NSCLC), many patients die of recurrent NSCLC. We investigated the association between gene methylation and recurrence of the tumor...
  35. pmc Epigenetic alteration of Wnt pathway antagonists in progressive glandular neoplasia of the lung
    Julien D F Licchesi
    Cancer Biology Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Carcinogenesis 29:895-904. 2008
    ....
  36. pmc Comparing the DNA hypermethylome with gene mutations in human colorectal cancer
    Kornel E Schuebel
    Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
    PLoS Genet 3:1709-23. 2007
    ....
  37. ncbi request reprint DNA methylation and complete transcriptional silencing of cancer genes persist after depletion of EZH2
    Kelly M McGarvey
    The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
    Cancer Res 67:5097-102. 2007
    ..These results suggest that EZH2 can modulate transcription of basally expressed genes but not silent genes that are densely DNA methylated...
  38. doi request reprint Promoter methylation of genes in and around the candidate lung cancer susceptibility locus 6q23-25
    Mathewos Tessema
    Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA
    Cancer Res 68:1707-14. 2008
    ..Together, our results indicate that frequent inactivation of multiple candidate tumor suppressor genes within chromosome 6q likely contributes to development of sporadic lung cancer...
  39. ncbi request reprint Hypermethylation of ASC/TMS1 is a sputum marker for late-stage lung cancer
    Emi Ota Machida
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Cancer Res 66:6210-8. 2006
    ..Thus, hypermethylation of ASC/TMS1 is a marker for late-stage lung cancer and, in sputum, could predict prognosis in patients resected for early-stage disease...
  40. ncbi request reprint Hypermethylation of a small CpGuanine-rich region correlates with loss of activator protein-2alpha expression during progression of breast cancer
    Donna B Douglas
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Cancer Res 64:1611-20. 2004
    ....
  41. ncbi request reprint Gene silencing in cancer in association with promoter hypermethylation
    James G Herman
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins Medical Institutions, Baltimore, USA
    N Engl J Med 349:2042-54. 2003
  42. ncbi request reprint Inhibition of DNA methylation and histone deacetylation prevents murine lung cancer
    Steven A Belinsky
    Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive SE, Albuquerque, New Mexico 87108, USA
    Cancer Res 63:7089-93. 2003
    ..Thus, reduction in DNMT and histone deacetylase activities that likely block epigenetically mediated gene silencing might provide a novel clinical strategy to help prevent the leading cause of cancer death in the United States...
  43. pmc GATA-4 and GATA-5 transcription factor genes and potential downstream antitumor target genes are epigenetically silenced in colorectal and gastric cancer
    Yoshimitsu Akiyama
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Mol Cell Biol 23:8429-39. 2003
    ..Selection for silencing of both upstream transcription factors and their target genes in GI cancers could indicate that epigenetic silencing of the involved genes provides a summated contribution to tumor progression...
  44. ncbi request reprint Prognostic importance of promoter hypermethylation of multiple genes in esophageal adenocarcinoma
    Malcolm V Brock
    Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
    Clin Cancer Res 9:2912-9. 2003
    ..The association of clinicopathological and biomolecular risk factors to survival and recurrence was performed using the Log-rank test and Cox proportional hazards model for multivariate analysis...
  45. ncbi request reprint Aberrant promoter methylation of the transcription factor genes PAX5 alpha and beta in human cancers
    William A Palmisano
    Lovelace Respiratory Research Institute, Lung Cancer Program, 2425 Ridgecrest Avenue S E, Albuquerque, New Mexico 87108, USA
    Cancer Res 63:4620-5. 2003
    ..Recent studies have demonstrated the importance of PAX5 gene alterations in human cancer. Our results are the first to identify aberrant promoter methylation as a common mechanism for dysregulation of these genes in solid tumors...
  46. ncbi request reprint Hedgehog signaling: progenitor phenotype in small-cell lung cancer
    D Neil Watkins
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, 1650 Orleans Street, Room 572, Baltimore, MD 21231, USA
    Cell Cycle 2:196-8. 2003
    ..How Hh signaling works to promote the malignant phenotype promises to be as important biologically as the promise of Hh pathway inhibitors are clinically...
  47. ncbi request reprint Hedgehog signalling within airway epithelial progenitors and in small-cell lung cancer
    D Neil Watkins
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Nature 422:313-7. 2003
    ..This requirement for Hh pathway activation identifies a common lethal malignancy that may respond to pharmacological blockade of the Hh signalling pathway...
  48. ncbi request reprint Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene
    Kurtis E Bachman
    The Howard Hughes Medical Institute, The Sidney Kimmel Comprehensive Cancer Center, and Program in Cellular and Molecular Medicine, The Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cancer Cell 3:89-95. 2003
    ..These results have important implications for understanding the biochemical events underlying the silencing of tumor suppressor genes and the resultant growth suppression...
  49. ncbi request reprint Dependence of histone modifications and gene expression on DNA hypermethylation in cancer
    Jill A Fahrner
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University, Baltimore, Maryland 21231 1000, USA
    Cancer Res 62:7213-8. 2002
    ..Our findings suggest a new paradigm-DNA methylation may directly, or indirectly by inhibiting transcription, maintain key repressive elements of the histone code at a hypermethylated gene promoter in cancer...
  50. ncbi request reprint Hypermethylation-associated Inactivation of the Cellular Retinol-Binding-Protein 1 Gene in Human Cancer
    Manel Esteller
    The Johns Hopkins Oncology Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Cancer Res 62:5902-5. 2002
    ..Epigenetic disruption of CRBP1 is a common event in human cancer that may have important implications for cancer prevention and treatment using retinoids...
  51. ncbi request reprint A genomic screen for genes upregulated by demethylation and histone deacetylase inhibition in human colorectal cancer
    Hiromu Suzuki
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Nat Genet 31:141-9. 2002
    ....
  52. ncbi request reprint DNMT1 and DNMT3b cooperate to silence genes in human cancer cells
    Ina Rhee
    The Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Nature 416:552-6. 2002
    ..Here we demonstrate that two enzymes cooperatively maintain DNA methylation and gene silencing in human cancer cells, and provide compelling evidence that such methylation is essential for optimal neoplastic proliferation...
  53. pmc Enzymatic regional methylation assay: a novel method to quantify regional CpG methylation density
    Oliver Galm
    Oncology Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Genome Res 12:153-7. 2002
    ..We demonstrate a use of this technique in determining the methylation density of the promoter region of the tumor suppressor gene p15INK4B and changes that occur after treatment with demethylating agents...
  54. ncbi request reprint Hematopoietic stem cells convert into liver cells within days without fusion
    Yoon Young Jang
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Nat Cell Biol 6:532-9. 2004
    ..Notably, liver function was restored 2-7 d after transplantation. We conclude that HSCs contribute to the regeneration of injured liver by converting into functional hepatocytes without fusion...
  55. ncbi request reprint CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1
    Angela H Ting
    Program in Cellular and Molecular Medicine, Cancer Biology Division, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University, Baltimore, Maryland 21231, USA
    Nat Genet 36:582-4. 2004
    ..Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1...
  56. ncbi request reprint Reversal of gene silencing as a therapeutic target for cancer--roles for DNA methylation and its interdigitation with chromatin
    Stephen B Baylin
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Room 544, The Cancer Research Center, 1650 E Orleans, Baltimore, MD 21231, USA
    Novartis Found Symp 259:226-33; discussion 234-7, 285-8. 2004
    ....
  57. pmc Inhibition of SIRT1 reactivates silenced cancer genes without loss of promoter DNA hypermethylation
    Kevin Pruitt
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, United States of America
    PLoS Genet 2:e40. 2006
    ....
  58. ncbi request reprint Silenced tumor suppressor genes reactivated by DNA demethylation do not return to a fully euchromatic chromatin state
    Kelly M McGarvey
    Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cancer Res 66:3541-9. 2006
    ..This finding has important implications for the translational goal of reactivating aberrantly silenced cancer genes as a therapeutic maneuver...
  59. ncbi request reprint Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohort
    Steven A Belinsky
    Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87108, USA
    Cancer Res 66:3338-44. 2006
    ....
  60. ncbi request reprint Angiostatic activity of DNA methyltransferase inhibitors
    Debby M E I Hellebrekers
    Angiogenesis Laboratory, Research Institute for Growth and Development, Department of Pathology, Maastricht University and University Hospital, P O Box 5800, 6202 AZ Maastricht, The Netherlands
    Mol Cancer Ther 5:467-75. 2006
    ..In conclusion, our data show that DNMT inhibitors have angiostatic activity in addition to their inhibitory effects on tumor cells. This dual action of these compounds makes them promising anticancer therapeutics...
  61. ncbi request reprint The fundamental role of epigenetics in hematopoietic malignancies
    Oliver Galm
    Medizinische Klinik IV, Universitaetsklinikum Aachen, 52074 Aachen, Germany
    Blood Rev 20:1-13. 2006
    ..The ongoing exploration of basic events involved in altered gene transcription patterns and continued clinical investigative studies are helping to develop novel strategies for the diagnosis, prevention, and treatment of human cancer...
  62. ncbi request reprint Differential requirement for DNA methyltransferase 1 in maintaining human cancer cell gene promoter hypermethylation
    Angela H Ting
    Program in Cellular and Molecular Medicine, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cancer Res 66:729-35. 2006
    ..These observations suggest that human cancer cells may differ in their reliance on DNMT1 for maintaining DNA methylation...
  63. ncbi request reprint Tumor suppressor HIC1 directly regulates SIRT1 to modulate p53-dependent DNA-damage responses
    Wen Yong Chen
    Cancer Biology Program, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, Maryland 21231, USA
    Cell 123:437-48. 2005
    ....
  64. ncbi request reprint Can we improve the cytologic examination of malignant pleural effusions using molecular analysis?
    Malcolm V Brock
    Division of Thoracic Surgery, Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Ann Thorac Surg 80:1241-7. 2005
    ..Deoxyribonucleic acid (DNA) methylation is a robust strategy for detecting cancer early in tissue. We hypothesized that DNA methylation would be more sensitive in diagnosing patients with malignant pleural effusions than cytology...
  65. pmc Short double-stranded RNA induces transcriptional gene silencing in human cancer cells in the absence of DNA methylation
    Angela H Ting
    Graduate Program in Cellular and Molecular Medicine, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland 21231, USA
    Nat Genet 37:906-10. 2005
    ..Moreover, we could accomplish such silencing in a cancer cell line genetically modified to lack virtually any capacity to methylate DNA...
  66. ncbi request reprint Surgical resection of limited disease small cell lung cancer in the new era of platinum chemotherapy: Its time has come
    Malcolm V Brock
    Division of Thoracic Surgery, Department of Surgery, The Johns Hopkinds Medical Institutions, Baltimore, MD, USA
    J Thorac Cardiovasc Surg 129:64-72. 2005
    ..Although resection is not the standard of care in treating small cell lung cancer, new platinum drugs and modern staging have allowed the role of surgery to be reevaluated...
  67. ncbi request reprint Inactivation of tumor suppressor genes: choice between genetic and epigenetic routes
    Wen Yong Chen
    Cancer Biology Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland, USA
    Cell Cycle 4:10-2. 2005
    ..The value of such a model and the implications of the findings for choice of epigenetically versus genetically determined loss of gene function in cancer are discussed...
  68. ncbi request reprint Hypermethylation of the GATA genes in lung cancer
    Mingzhou Guo
    Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, MD 21287, USA
    Clin Cancer Res 10:7917-24. 2004
    ..In lung cancer, DNA hypermethylation is known to be a common event...
  69. ncbi request reprint Epigenetic and genetic loss of Hic1 function accentuates the role of p53 in tumorigenesis
    Wenyong Chen
    Cancer Biology Program, Johns Hopkins Medical Institutions, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cancer Cell 6:387-98. 2004
    ..Our results indicate the importance of genes altered only through epigenetic mechanisms in cancer progression in conjunction with genetically modified tumor suppressor genes...
  70. ncbi request reprint Promoter hypermethylation of resected bronchial margins: a field defect of changes?
    Mingzhou Guo
    Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
    Clin Cancer Res 10:5131-6. 2004
    ..We hypothesized that DNA promoter hypermethylation changes at bronchial margins could be detected in patients with no histological evidence of malignancy and that they would reflect epigenetic events in the primary tumor...
  71. ncbi request reprint Hypermethylation of the DNA repair gene O(6)-methylguanine DNA methyltransferase and survival of patients with diffuse large B-cell lymphoma
    Manel Esteller
    Division of Cancer Biology, The Johns Hopkins Oncology Center, Baltimore, MD 21231, USA
    J Natl Cancer Inst 94:26-32. 2002
    ..MGMT promoter hypermethylation is a favorable prognostic marker in patients with brain tumors treated with alkylating agents...

Research Grants49

  1. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 1993
    ..Finally, a model for increased cellular DNA methylating capacity has been constructed to study the resultant molecular changes and the phenotypic consequences for eukaryotic cells...
  2. The Environment and Epigenetic Gene Silencing in Cancer
    Stephen Baylin; Fiscal Year: 2007
    ..These, in turn, may be driven, and/or modulated, by key environmental factors. Thus, it is inferred that a key to cancer prevention may be to induce reversal of, or block, epigenetic gene silencing. ..
  3. Developmental pathways, cancer, and DNA hypermethylation
    Stephen Baylin; Fiscal Year: 2006
    ..Animal models will then be utilized to extrapolate our results to in vivo tumor progression settings. ..
  4. Cancer Genome Characterization Center at Johns Hopkins
    Stephen Baylin; Fiscal Year: 2007
    ..This project has direct relevance to public health. Precise knowledge of the type and frequency of the major cancer causing alterations will allow the best molecular targets to be selected for new therapy development. ..
  5. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2006
    ..abstract_text> ..
  6. Developmental pathways, cancer, and DNA hypermethylation
    Stephen Baylin; Fiscal Year: 2005
    ..Animal models will then be utilized to extrapolate our results to in vivo tumor progression settings. ..
  7. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2005
    ..abstract_text> ..
  8. The Environment and Epigenetic Gene Silencing in Cancer
    Stephen Baylin; Fiscal Year: 2006
    ..These, in turn, may be driven, and/or modulated, by key environmental factors. Thus, it is inferred that a key to cancer prevention may be to induce reversal of, or block, epigenetic gene silencing. ..
  9. Cancer Genome Characterization Center at Johns Hopkins
    Stephen Baylin; Fiscal Year: 2006
    ..This project has direct relevance to public health. Precise knowledge of the type and frequency of the major cancer causing alterations will allow the best molecular targets to be selected for new therapy development. ..
  10. SPORE IN LUNG CANCER
    Stephen Baylin; Fiscal Year: 2006
    ....
  11. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen B Baylin; Fiscal Year: 2010
    ..We investigate whether this change can foster cancer initiation and the molecular mechanisms though which it arises. ..
  12. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2004
    ..abstract_text> ..
  13. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 1999
    ..We will attempt to use this maneuver to model selected human tumor progression steps. We will also evaluate the phenotypic consequences of general, and tissue specific, overexpresslon of an exogenous DNAMTase gene In transgenic mice. ..
  14. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 1990
    ..Finally, a model for increased cellular DNA methylating capacity has been constructed to study the resultant molecular changes and the phenotypic consequences for eukaryotic cells...
  15. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 1991
    ..Finally, a model for increased cellular DNA methylating capacity has been constructed to study the resultant molecular changes and the phenotypic consequences for eukaryotic cells...
  16. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2002
    ..We will attempt to use this maneuver to model selected human tumor progression steps. We will also evaluate the phenotypic consequences of general, and tissue specific, overexpresslon of an exogenous DNAMTase gene In transgenic mice. ..
  17. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2001
    ..We will attempt to use this maneuver to model selected human tumor progression steps. We will also evaluate the phenotypic consequences of general, and tissue specific, overexpresslon of an exogenous DNAMTase gene In transgenic mice. ..
  18. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2003
    ..We will attempt to use this maneuver to model selected human tumor progression steps. We will also evaluate the phenotypic consequences of general, and tissue specific, overexpresslon of an exogenous DNAMTase gene In transgenic mice. ..
  19. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2009
    ..We investigate whether this change can foster cancer initiation and the molecular mechanisms though which it arises. ..
  20. The Environment and Epigenetic Gene Silencing in Cancer
    Stephen Baylin; Fiscal Year: 2009
    ..These, in turn, may be driven, and/or modulated, by key environmental factors. Thus, it is inferred that a key to cancer prevention may be to induce reversal of, or block, epigenetic gene silencing. ..
  21. DNA Methyltrasferase Gene Expression in Colon Cancer
    Stephen Baylin; Fiscal Year: 2009
    ..We investigate whether this change can foster cancer initiation and the molecular mechanisms though which it arises. ..
  22. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2000
    ..We will attempt to use this maneuver to model selected human tumor progression steps. We will also evaluate the phenotypic consequences of general, and tissue specific, overexpresslon of an exogenous DNAMTase gene In transgenic mice. ..
  23. DNA METHYLTRANSFERASE GENE EXPRESSION IN COLON CANCER
    Stephen Baylin; Fiscal Year: 2006
    ..Weaknesses include the descriptive nature of the work, although the addition/improvement of aim three begins to address this. ..
  24. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 1992
    ..Finally, a model for increased cellular DNA methylating capacity has been constructed to study the resultant molecular changes and the phenotypic consequences for eukaryotic cells...
  25. METHYLATION OF THE CALCITONIN GENE IN HUMAN TUMORS
    Stephen Baylin; Fiscal Year: 2007
    ..abstract_text> ..
  26. Developmental pathways, cancer, and DNA hypermethylation
    Stephen Baylin; Fiscal Year: 2007
    ..Animal models will then be utilized to extrapolate our results to in vivo tumor progression settings. ..
  27. SPORE IN LUNG CANCER
    Stephen Baylin; Fiscal Year: 2007
    ....
  28. Developmental pathways, cancer, and DNA hypermethylation
    Stephen Baylin; Fiscal Year: 2009
    ..Animal models will then be utilized to extrapolate our results to in vivo tumor progression settings. ..
  29. DNA METHYLTRANSFERASE GENE EXPRESSION IN COLON CANCER
    Stephen Baylin; Fiscal Year: 2007
    ..Weaknesses include the descriptive nature of the work, although the addition/improvement of aim three begins to address this. ..
  30. DNA Methyltrasferase Gene Expression in Colon Cancer
    Stephen B Baylin; Fiscal Year: 2010
    ..We investigate whether this change can foster cancer initiation and the molecular mechanisms though which it arises. ..